CN108849749B - Method for trapping and killing parasitic wasps of alfalfa cutting leaf wasps - Google Patents
Method for trapping and killing parasitic wasps of alfalfa cutting leaf wasps Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/04—Attracting insects by using illumination or colours
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/20—Poisoning, narcotising, or burning insects
- A01M1/2005—Poisoning insects using bait stations
- A01M1/2016—Poisoning insects using bait stations for flying insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Insects & Arthropods (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a parasitic wasp trapping and killing method of alfalfa leaf cutting wasps, which comprises the following steps: 1) placing an alfalfa leaf cutting bee hatching tray in an artificial climate chamber, placing a water tray below the bee hatching tray, installing a black light lamp between the bee hatching tray and the water tray, wherein the distance between the black light lamp and the water tray is about 10cm, and periodically replacing water in the water tray; 2) the screened 1.5 percent prallethrin trapping and killing agent device is arranged in a climatic chamber, and the dosage is 3.25ml/d/10m2(ii) a 3) And (3) placing the alfalfa leaf cutting bee cocoons into a phytotron 2d, starting a black light lamp and a trapping and killing agent volatilization device, observing at regular time every day, counting the number of parasitic bees in a water tray and an incubation bee tray respectively, and turning off the black light lamp and moving out the trapping and killing agent device when observing the parasitic bees which are not feathered within 2d continuously. The invention can trap and kill parasitic wasps by about 95 percent, is safe to the cocoons of the alfalfa leaf cutting wasps and does not influence the normal emergence of the alfalfa leaf cutting wasps.
Description
Technical Field
The invention belongs to the technical field of agricultural insect control, relates to artificial breeding of alfalfa leaf cutting bees, and particularly relates to a method for trapping and killing parasitic wasps of alfalfa leaf cutting bees. The invention adopts the method of combining the black light lamp with the chemical trapping agent to trap and kill parasitic wasps before or at the same time of emergence of the alfalfa cutting leaf wasps.
Background
Alfalfa (Medicago sativa) Also called alfalfa or ordinary alfalfa, is called the king of pasture due to its abundant proteins, amino acids, vitamins and various nutritional ingredients. One of the outstanding problems encountered in the promotion of alfalfa cultivation is the lack of sufficient numbers of seeds. One of the important experiences in high yield is the use of alfalfa leaf cutting bees to pollinate alfalfa. In countries with developed animal husbandry, the use of alfalfa leaf cutting bees for pollination of alfalfa has become one of the indispensable measures in the production process of alfalfa seeds. The alfalfa pollinated by the alfalfa leaf cutting bees not only increases the seed yield by times, but also improves the quality.
Alfalfa leaf cutting bee: (Megachile rotundataF.) the family Apidae, native Western Asia and Europe, are now distributed in many countries and regions in continents of the world. The alfalfa leaf cutting bees are one of the peak weights of efficient pollination of leguminous plants such as alfalfa and the like, and great economic benefits are brought to alfalfa seed producers. At present, the artificial propagation of the alfalfa leaf cutting bees is to nest and collect pollen and nectar to propagate offspring in a special porous polyethylene foam board. Under the initial natural condition, the enemies of alfalfa leaf cutting bees are rare, but because ten thousand of cocoons can be built in a nest plate with a small volume, the unified management of the nest plate, the centralized storage of the cocoons separated in autumn, the centralized heating of the cocoons in spring and the like create favorable conditions for the increase of natural enemy populations. It is reported that the recorded natural enemies of alfalfa secant bees have reached several tens in north america. China has found various natural enemies of alfalfa secatella, the main natural enemy of which is single-tooth leg long-tail wasp ((C))Monodontomerus minorRatz.) and Chouioia wasp (bee: (bee) ((bee))Tetrastichussp.). In a long termIn inspection, the breeding places of the alfalfa cutting wasps are relatively fixed in recent years, and as the breeding amount of the alfalfa cutting wasps increases, some secondary natural enemies gradually rise to primary natural enemies, so that certain harm is caused to the alfalfa cutting wasps.
At present, the work of artificial breeding of the alfalfa cutting wasps in China is still imperfect and stable. Alfalfa leaf cutting bees often cause diseases and insect pests due to air conduction in rainy days or the emergence of cocoons is blocked, particularly parasitic bees. Because the parasitic wasps are multi-embryo reproduction, 2 generations of reproduction can be completed in the emergence period of the alfalfa cut-leaf wasps, the parasitic rate on the alfalfa cut-leaf wasps can reach over 75 percent, serious harm is caused to the propagation of the alfalfa cut-leaf wasps population, and the propagation quantity of the alfalfa cut-leaf wasps is reduced. As for the trapping and killing technology of the parasitic wasps of the alfalfa cutting leaf wasps, dichlorvos and incandescent lamps are generally used at home and abroad for trapping and killing, and because the dichlorvos are decomposed by heating, toxic gases of phosphorus oxide and chloride are released, the toxic gases are improper to use, people are easy to be poisoned, and meanwhile, the parasitic wasps are extremely toxic; after the use, ventilation is needed, the temperature and the humidity of an artificial climate chamber are easily reduced, and the development of the alfalfa leaf cutting bees with longer emergence period is not favorable.
Disclosure of Invention
The invention provides a method for trapping and killing parasitic wasps of alfalfa cutting wasps, aiming at the technical defect of trapping and killing parasitic wasps of alfalfa cutting wasps. The method adopts the method of combining the black light lamp with the chemical trapping agent, the parasitic wasp trapping and killing and the alfalfa leaf cutting wasp emergence are synchronous, the method is safe for operators and the alfalfa leaf cutting wasps, and after the method is used, an artificial climate chamber does not need to be ventilated, so that the normal development of the alfalfa leaf cutting wasps is not influenced.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a method for trapping and killing parasitic wasps of alfalfa cutting wasps is disclosed, which traps and kills parasitic wasps while the alfalfa cutting wasps eclosion. The method for trapping and killing the insects comprises the following steps.
Step 1, installing a bee hatching tray for emergence of alfalfa leaf cutting bees in an artificial climate chamber, placing alfalfa leaf cutting bee cocoons in the bee hatching tray, placing a water tray below the bee hatching tray, installing a black light lamp between the bee hatching tray and the water tray, wherein the distance between the black light lamp and the water tray is about 10cm, the water tray is filled with water, and the water in the water tray is periodically replaced.
And 2, installing a chemical attractant volatilization device in the artificial climate chamber, wherein the chemical attractant volatilization device is used for containing a chemical attractant, and the dosage of the chemical attractant is capable of killing parasitic bees or reducing the activity of the parasitic bees, but is suitable for not influencing the normal emergence of the alfalfa leaf cutting bees.
And 3, starting from the next day when the alfalfa leaf cutting bee cocoons are placed, starting a black light lamp, simultaneously placing a chemical attractant into a chemical attractant volatilization device, observing at regular time every day, respectively counting the number of parasitic bees in the water tray and the incubation bee tray, and removing the parasitic bees after the observation is finished.
As one of the preferable embodiments of the technical scheme of the invention, the implementation of the trapping and killing method is carried out in a climatic chamber. In order to ensure the emergence of the alfalfa cutting leaf bees without influencing the normal development of the alfalfa cutting leaf bees, the environmental conditions in the artificial climate chamber are set to be constant temperature of 30 ℃ and relative humidity of 60%. In the invention, one of the important measures for trapping and killing the parasitic wasps of the alfalfa cutting wasps is to install a chemical attractant volatilization device in a climatic chamber, wherein the device is used for containing the chemical attractant. In order to kill parasitic wasps or reduce the activity of parasitic wasps without affecting the normal emergence of alfalfa leaf cutting wasps, the invention strictly controls the installation amount of chemical attractant volatilizing devices in the artificial climate chamber, and the installation amount is calculated according to the floor area of the artificial climate chamber and is 10 m/per unit2A chemical attractant volatilization device is installed.
In order to eliminate the defect of trapping and killing the parasitic wasps of the alfalfa leaf cutting bees by using the dichlorvos and the incandescent lamp, the method adopts the prallethrin with lower toxicity as the effective component of the chemical trapping agent for the first time. In specific implementation, the chemical attractant is volatile electric mosquito repellent liquid, and the effective component of the electric mosquito repellent liquid is 1.5% prallethrin. The prallethrin belongs to pyrethrin insecticides, has strong contact killing effect, and is mainly used for processing mosquito-repellent incense, electric heating mosquito-repellent incense, liquid mosquito-repellent incense and spraying agent, and preventing and controlling household pests such as houseflies, mosquitoes, lice, cockroaches and the like. The invention strictly uses the prallethrin in the artificial climate roomIf the dosage is too small, parasitic wasps cannot be killed effectively or the activity of the parasitic wasps is reduced; if the dosage is too large, the normal emergence of the alfalfa leaf cutting bees is influenced, so that the alfalfa leaf cutting bees die. Preferably, the amount of the volatile electric mosquito-repellent incense solution is 0.8-3.25 ml/d/10m2。
Besides the chemical trapping agent, the invention also selects a mode of combining a 15W black light lamp with the chemical trapping agent to trap and kill parasitic wasps in a more effective mode. The invention replaces the incandescent lamp with the black light lamp, and the black light lamp has stronger insect-attracting effect on insects and is incomparable with the incandescent lamp. The insect attracting principle of the black light lamp is that the compound eye of the insect is very sensitive to the ultraviolet radiation with the wavelength of 365nm, especially flying insects. Most phototactic insects prefer 330-400 nm ultraviolet light, and particularly lepidoptera and coleoptera insects are more sensitive to this wavelength band. In a specific implementation, the black-light lamp may be used for 24 hours per day.
The water tray is used for catching the parasitic wasps induced by the black light lamp, and the water tray is filled with water, so that the parasitic wasps can be effectively prevented from escaping. In the process of using the technology of the invention, parasitic wasps falling into the water tray need to be cleaned regularly, and the water is ensured to be changed once a day.
In a preferred embodiment of the technical scheme of the invention, considering the using dosage of the prallethrin, the chemical attractant is preferably used for 8-24 hours per day.
In addition, the invention can flexibly change the implementation of the trapping and killing method according to the number of parasitic wasps. For example, if no parasitic wasp of the alfalfa leaf cutting wasp appears in 48h in the water tray, indicating that the parasitic wasp is almost absent, the trapping treatment by using the chemical trapping agent can be stopped.
In a preferred embodiment of the technical scheme of the invention, the trapping treatment using the chemical trapping agent is stopped from the second day when the hornet cocoons of the alfalfa leaf cutting bees are put in to the end of the complete feather of the parasitic wasps of the alfalfa leaf cutting bees.
The emergence period of the parasitic wasp is generally 11d, and in the specific implementation, after the parasitic wasp is subjected to trapping and killing treatment for 8-10 d, the complete emergence of the parasitic wasp of the alfalfa leaf cutting wasp is finished. However, the emergence period of the alfalfa leaf cutting bees is long, the emergence can be completed only in about 25 days generally, and after the parasitic wasps of the alfalfa leaf cutting bees are completely emerged, the emergence condition of the alfalfa leaf cutting bees is continuously observed until the emergence of the alfalfa leaf cutting bees is completely completed.
Compared with the prior art, the method for trapping and killing the parasitic wasps of the alfalfa cutting wasps has at least the following beneficial effects or advantages:
the invention adopts the method of combining the black light lamp with the prallethrin for the first time, and realizes the trapping and killing of parasitic wasps while the alfalfa leaf cutting wasps eclosion. The method is carried out in an artificial climate chamber, a 15W black light lamp and a water tray are sequentially arranged below an incubation plate for placing alfalfa leaf cutting bee cocoons, a certain number of chemical trapping and killing agent volatilization devices are arranged in the artificial climate chamber, electric mosquito repellent liquid containing 1.5% of prallethrin is added into the chemical trapping and killing agent volatilization devices, and the using amount of the electric mosquito repellent liquid is 0.8-3.25 ml/d/10m2(ii) a Starting from the second day of emergence of the alfalfa leaf cutting bees, the black light lamp is started, meanwhile, the electric mosquito repellent liquid is used, the black light lamp can be used for 24 hours every day until no parasitic bees appear in the water tray, and the trapping and killing of the parasitic bees can be completed. By adopting the method, the parasitic rate of the parasitic wasps is controlled within 5 percent, the trapping and killing effect is quite obvious, simultaneously the method is safe to the alfalfa leaf cutting wasps, and the normal emergence of the alfalfa leaf cutting wasps is not influenced. The method for trapping and killing the parasitic wasps of the alfalfa leaf cutting bees has the advantages of simple technology, easy popularization and low cost, and effectively controls the harm of the parasitic wasps to the alfalfa leaf cutting bees.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
The embodiment provides a method for trapping and killing parasitic wasps of alfalfa cutting wasps. The trapping and killing method adopts a mode that the black light lamp is matched with the prallethrin for the first time, the black light lamp is used for trapping parasitic wasps, and the prallethrin is used for killing the parasitic wasps or reducing the activity of the parasitic wasps. The trapping and killing method is carried out in an artificial climate room, and the trapping and killing of parasitic wasps and the emergence of alfalfa leaf cutting wasps are synchronous.
(1) The environmental conditions in the artificial climate chamber are set to be constant temperature of 30 ℃ and relative humidity of 60%, and suitable environmental conditions are provided for emergence of the Lucerne Sesamum sp cocoons. The alfalfa leaf cutting bee cocoons are placed in a bee hatching tray, and the bee hatching tray is installed in an artificial climate chamber. A15W black light lamp and a water tray are sequentially arranged below the bee hatching tray, and the distance between the black light lamp and the water tray is about 10 cm. In a specific implementation, the black light lamp can be used for 24 hours every day, and the size of the water tray is the same as that of the bee hatching tray. The water tray is filled with water, and the water in the water tray is replaced periodically. Preferably, the water in the water tray is guaranteed to be changed once a day.
(2) A certain number of chemical attractant volatilization devices are also installed in the artificial climate chamber. The chemical attractant volatilization device is used for containing a chemical attractant, and the dosage of the chemical attractant is capable of killing parasitic wasps or reducing the mobility of the parasitic wasps, but is suitable for not influencing the normal emergence of the alfalfa leaf cutting wasps. In the process of using the technology of the embodiment, the area of the artificial climate chamber is calculated according to the floor area of the artificial climate chamber, and the floor area is calculated every 10m2A chemical attractant volatilization device is installed. The chemical trapping and killing agent is volatile electric mosquito repellent liquid, the effective component of the electric mosquito repellent liquid is 1.5% of prallethrin, and the dosage of the chemical trapping and killing agent is 0.8-3.25 ml/d/10m2。
(3) Starting from the next day when the alfalfa leaf cutting bee cocoons are placed, turning on a black light lamp, and simultaneously placing the electric mosquito repellent liquid into a chemical attractant volatilization device, wherein the daily use time of the electric mosquito repellent liquid is 8-24 hours. And observing at regular time every day, counting the number of parasitic wasps in the water tray and the hatching tray respectively, and removing the parasitic wasps after the observation is finished.
In other cases, the trapping treatment with the chemical attractant can be stopped when no parasitic wasp of the alfalfa leaf cutting wasp appears in the water pan within 48 h.
Stopping the trapping treatment by using the chemical trapping agent from the next day when the alfalfa leaf cutting bee cocoons are put in to the end of the complete feather of the parasitic bees of the alfalfa leaf cutting bees. After the parasitic wasp of the alfalfa leaf cutting wasp is completely emerged, considering that the emergence period of the alfalfa leaf cutting wasp is long, the emergence condition of the alfalfa leaf cutting wasp needs to be continuously observed until the emergence of the alfalfa leaf cutting wasp is completely finished.
Example 2
The method for trapping and killing the parasitic wasps of the alfalfa cutting wasps, which is described in the embodiment 1, is adopted to perform an emergence experiment of the parasitic wasps of the alfalfa cutting wasps, so that the effectiveness of the method for trapping and killing the parasitic wasps of the alfalfa cutting wasps, which is described in the embodiment 1, is verified.
(1) Test materials and facilities
1500 alfalfa leaf cutting bee cocoons, 5 hatching bee plates, 5 15W black light lamps (manufactured by Philips), 5 white water plates, electric mosquito repellent liquid (1.5% of prallethrin as an active ingredient), and DEET (culicidin) as a contrast chemical attractant.
Artificial climate chamber with 10m inside2And installing a chemical attractant volatilization device, and adding the electric mosquito repellent liquid or DEET into the chemical attractant volatilization device according to the experimental design. The environmental conditions in the climatic chamber were set at a constant temperature of 30 ℃ and a relative humidity of 60%.
(2) Design of experiments
The experimental setup was A, B, C three treatment groups, a chemical trap control DEET group and a blank control CK group. Each group is designed with 3 repetitions, each of which is 100 pieces of lucerne cut leaf bee cocoons.
The alfalfa leaf cutting bee cocoons are bred in the artificial climate chambers, and a black light lamp and a water tray are used for 24 hours in each artificial climate chamber. The water in the water tray was changed once a day.
Treatment group A: the daily use time of the electric mosquito repellent liquid is 8h, and the dosage is 0.8ml/d/10m2The electric mosquito repellent liquid is put in at a ratio of 9:00 and taken out at a ratio of 17:00 every day.
B, treatment group: the daily use time of the electric mosquito repellent liquid is 16h, and the dosage is 1.85ml/d/10m2The mosquito-repellent incense liquid is put in 17:00 every day and taken out 9:00 the next day.
Treatment group C: the daily use time of the electric mosquito repellent liquid is 24h, and the dosage is 3.25ml/d/10m2。
DEET group: the usage time of the culicifumide is 24 hours per day, and the dosage is 1.5ml/d/10m2。
And (3) CK group: only a black light lamp and a water tray were used.
All the above treatments are carried out until the emergence of parasitic wasps of the alfalfa leaf cutting wasps is no longer present.
(3) Observing and recording
According to the observation result of emergence of parasitic wasps of the alfalfa leaf cutting bees at the earlier stage, the number of the parasitic wasps is observed at 9:00 and 17:00 every day after the alfalfa leaf cutting bees are placed into the alfalfa leaf cutting bee cocoons for 2 days. And recording the number of escaping parasitic wasps when the parasitic wasps escape. Stopping each trapping and killing treatment till the parasitic wasps of the alfalfa leaf cutting wasps completely emerge (generally 8-10 days). Considering that the emergence period of parasitic wasps is generally 11d, while the emergence period of alfalfa leaf cutting bees generally needs about 25d to be completed, the emergence number of the alfalfa leaf cutting bees needs to be recorded continuously at 9:00 and 17:00 every day, and after the emergence of the alfalfa leaf cutting bees is finished, the observation and recording work is finished.
(4) Results and analysis
The results of the above treatments are shown in Table 1.
TABLE 1 statistics of trapping and killing results of parasitic wasps of alfalfa cutting leaf wasps
In table 1, the normal number refers to the number of hornet cocoons that are not parasitized by the parasitized bees and can normally emerge. The death number refers to the number of dead lucerne cut leaf bee cocoons caused by factors other than the death caused by parasitic wasps, such as mildew, chalkbrood disease, dry death or extrusion death.
As can be seen from table 1, A, B, C the scissoring hornet cocoons of the three treatment groups were significantly reduced in number by parasitic wasps. Although the DEET group has the culicin as a chemical attractant, the effect of preventing the parasites of the lucerne cut leaf wasp cocoons from being parasitized by the parasitism is not ideal. The CK group only uses a black light lamp and a water disc, has no effect of a chemical attractant, and the alfalfa leaf cutting bee cocoons have the largest parasitic number of parasitic bees, and have 22 particles on average. A. B, C the daily use time of the electric mosquito repellent liquid is increased, and the dosage of the electric mosquito repellent liquid is increased to effectively trap and kill parasitic wasps, when the daily use time of the electric mosquito repellent liquid is 24h, the dosage is 3.25ml/d/10m2Only 4 pieces of alfalfa leaf cutting bee cocoons are coveredAnd (4) parasitizing. The statistic results showed that A, B, C the three treatment groups were 2.00%, 2.36% and 1.33%, and the parasitic rates of the two controls were 6.33% and 7.33%, respectively. Therefore, the control effect of A, B, C three treatment groups is obviously higher than that of two controls, which shows that the technology of the invention has obvious effect on controlling the parasitic wasp of the alfalfa leaf cutting wasp.
The present invention has been further described with reference to the examples, but the present invention is not limited to the above-described embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (7)
1. The trapping and killing method of the parasitic wasps of the alfalfa cutting leaf wasps is characterized in that the trapping and killing method traps and kills the parasitic wasps before or simultaneously with the emergence of the alfalfa cutting leaf wasps, and the implementation of the trapping and killing method comprises the following steps:
step 1, placing an alfalfa leaf cutting bee hatching tray in an artificial climate chamber, placing alfalfa leaf cutting bee cocoons in the hatching tray, placing a water tray below the hatching tray, installing a black light lamp between the hatching tray and the water tray, wherein the distance between the black light lamp and the water tray is about 10cm, the water tray is filled with water, and the water in the water tray is replaced periodically;
step 2, installing the chemical attractant volatilization device in the artificial climate chamber, and calculating according to the floor area of the artificial climate chamber, wherein the chemical attractant volatilization device is arranged every 10m2Installing a chemical attractant volatilization device; the chemical trapping and killing agent volatilization device is used for containing a chemical trapping and killing agent, the chemical trapping and killing agent is 1.5% of prallethrin electric mosquito repellent liquid, and the dosage of the chemical trapping and killing agent is 0.8-3.25 ml/d/10m2Not only can kill parasitic wasps or reduce the activity of the parasitic wasps, but also does not influence the normal eclosion of the alfalfa leaf cutting wasps;
and 3, starting from the next day when the alfalfa leaf cutting bee cocoons are placed, starting a black light lamp, simultaneously placing a chemical attractant into a chemical attractant volatilization device, observing every day at regular time, and respectively counting the number of parasitic bees in the water tray and the incubation bee tray.
2. The method of claim 1, wherein the environmental conditions in the climatic chamber are set at a constant temperature of 30 ℃ and a relative humidity of 60%.
3. The method for trapping and killing autographa californica parasitic wasps according to claim 1, wherein the black light lamp is a 15W black light lamp, and the black light lamp is used for 24 hours each day; the water tray is filled with water.
4. The method for trapping and killing parasitic wasps of alfalfa cutting wasps according to claim 1, wherein the chemical trapping and killing agent is used for 8-24 hours per day.
5. The method for trapping and killing parasitic wasps of alfalfa leaf cutting bees according to claim 1, wherein the trapping and killing treatment with the chemical trapping and killing agent can be stopped when no parasitic wasp of alfalfa leaf cutting bees appears in 48 hours in the water tray.
6. The method for trapping and killing parasitic wasps of alfalfa leaf cutting bees according to claim 1, wherein the trapping treatment with the chemical trapping agent is stopped from the second day when the parasitic wasps of alfalfa leaf cutting bees are placed into the cocoon of alfalfa leaf cutting bees to the end of the complete feather of the parasitic wasps of alfalfa leaf cutting bees.
7. The method for trapping and killing parasitic wasps of alfalfa leaf cutting bees according to claim 6, wherein after the parasitic wasps of alfalfa leaf cutting bees are completely emerged, the emergence condition of alfalfa leaf cutting bees is continuously observed until the emergence of alfalfa leaf cutting bees is completely finished.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012154020A2 (en) * | 2011-05-06 | 2012-11-15 | Costilla Zarate Sonia Leticia | Insect control system |
| CN202873616U (en) * | 2012-07-14 | 2013-04-17 | 浙江森得保生物制品有限公司 | Insecticidal lamp special for wood-boring insects |
| CN204259676U (en) * | 2014-11-18 | 2015-04-15 | 杨宏婺 | One lures pest-catching device |
| CN205390076U (en) * | 2016-02-21 | 2016-07-27 | 榆林学院 | Jujube is eaten bud weevil adult and is trapped and killed device |
| CN206213071U (en) * | 2016-11-30 | 2017-06-06 | 沈阳农业大学 | A kind of trap of spotted wing drosophila and its natural enemy |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012154020A2 (en) * | 2011-05-06 | 2012-11-15 | Costilla Zarate Sonia Leticia | Insect control system |
| CN202873616U (en) * | 2012-07-14 | 2013-04-17 | 浙江森得保生物制品有限公司 | Insecticidal lamp special for wood-boring insects |
| CN204259676U (en) * | 2014-11-18 | 2015-04-15 | 杨宏婺 | One lures pest-catching device |
| CN205390076U (en) * | 2016-02-21 | 2016-07-27 | 榆林学院 | Jujube is eaten bud weevil adult and is trapped and killed device |
| CN206213071U (en) * | 2016-11-30 | 2017-06-06 | 沈阳农业大学 | A kind of trap of spotted wing drosophila and its natural enemy |
Non-Patent Citations (1)
| Title |
|---|
| 甘肃省引进苜蓿切叶蜂孵化试验;吴衍庆 等;《草业科学》;20010430;第18卷(第2期);第18-20页 * |
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