CN107397975A - Medical use anti-infection Wound dressing and preparation method thereof - Google Patents

Abstract

The present invention provides medical use anti-infection Wound dressing and preparation method thereof, wherein, Medical wounded surface dressing is as prepared by following raw material：Bacteria cellulose, PAHy, polyvinyl alcohol, Sodium Polyacrylate, tragacanth, allantoin, galacturonic acid, tumer how quinhydrones, Kaempferol glucuronide, plant water extract, polyoxyethylene poly-oxygen propylene aether block copolymer, polyethyleneglycol diglycidylether, glycerine, distilled water.The antibacterial anti-infection of Medical wounded surface dressing prepared by the present invention is good, has broad spectrum antibacterial；And the healing cycle of this dressing was controlled within one week, promote to be cured significant effect, while the smooth exquisiteness of skin surface is regenerated after healing, produced without scar, outstanding to the healing ability of the surface of a wound, healing effect is good, is applicable to a variety of wound treatments such as wound, burn, ulcer.

Description

Medical use anti-infection Wound dressing and preparation method thereof

Technical field

The invention belongs to medical material tech field, and in particular to medical use anti-infection Wound dressing and preparation method thereof.

Background technology

Skin is the maximum organ of human body, and it is wrapped in human body whole surface, and with digestion, breathing, uropoiesis, reproductive system The mucous epithelium of collar extension is connected, and so as to form the optimal coat of human body, protection internal organ are invaded with the extraneous various factors of prevention Attack.Because of situations such as baiting, extruding, rubbing, falling and touch, perform the operation and occur hurting, scratch, hemostasis, injure by a crashing object, squeeze and hinder, abrade, split Hinder, stab, the wound such as mosquito is bitten, burning is scalded, these surface of a wound must timely processing, otherwise can occur due to the intrusion of pathogenetic bacteria Pyogenic infection, red, swollen, the hot, pain of local appearance and dysfunction are will also result in, because bacterium and toxin enter blood when serious Circulation can cause the illnesss such as septicemia, lockjaw and toxaemia, serious threat patient vitals health.

The main form for taking medical dressing external application for the treatment of at present clinically to wound.Traditional dressing is for example natural or closes Into bandage, absorbent cotton and gauze, its major function are to allow wound fluid to evaporate as early as possible, keep the drying of wound, prevent simultaneously Invasion of the harmful bacteria to wound.It is that wound exists however as the theoretical proposition of the progress of medical technology, especially wet union Can faster it be healed in the environment of warm and moist, new pattern compress resists various microorganisms with preventing that wound is over-drying Prevent two degree of infection of wound, wound may be resisted and damaged by mechanical factor, pollution and chemical stimulation；Greatly reduce electrolyte and Loss of power, comprehensive protection is carried out to wound, therefore, Medical dressing gradually instead of traditional dressing.It is new at present Medical dressing can be divided mainly into four kinds of fibrous type, aquogel type, film-type and sponge-type by its form, and wherein aerogel dressing has There are more preferable water imbibition, breathable moisture permeability and biocompatibility, and its raw material sources is extensive, price is relatively cheap therefore this kind of Dressing research and application are more extensive.Although the research at present on Wound dressing is more, most aerogel dressing is anti- Infection biocidal property, preventing from scar, rush are cured effect and absorbency etc. and some defects and deficiency more or less be present, in certain journey Its application is limited on degree.

The content of the invention

Therefore, the present invention provides medical use anti-infection Wound dressing and preparation method thereof, solves above-mentioned technology in the prior art Problem.

Therefore, the present invention provides medical use anti-infection Wound dressing, as prepared by following raw material, it is calculated as by weight：Bacterium Cellulose 5-15 parts, PAHy 0.5-3 parts, polyvinyl alcohol 0.1-1.4 parts, Sodium Polyacrylate 0.3-1 parts, tragacanth 2-6 Part, allantoin 0.6-2.5 parts, galacturonic acid 0.4-1.8 parts, tumer how quinhydrones 0.03-0.15 parts, Kaempferol grape alditol It is sour glycosides 0.1-0.28 parts, plant water extract 1.2-3.2 parts, polyoxyethylene poly-oxygen propylene aether block copolymer 2.4-7.3 parts, poly- Ethylene glycol diglycidylether 0.5-4 parts, glycerine 15-28 parts, distilled water 26-40 parts；

The plant water extract is Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine section wood and the water extract of peppermint.

The preparation process of the bacteria cellulose is：Strain is added in seed culture medium, 10- is cultivated at 20-30 DEG C 24h, inoculate into fermentation medium, 10-24h is cultivated at 20-30 DEG C, decantation, produces bacteria cellulose；Wherein, it is described Strain is to acetify bacillus, Pseudomonas cepacia or grape saccharobacillus；The seed culture medium includes glucose, sweet potato starch, pancreas Peptone, disodium hydrogen phosphate, citric acid, magnesium sulfate, cobalt chloride；The fermentation medium includes glucose, dusty yeast, tryptose Peptone, disodium hydrogen phosphate, lactic acid, acetic acid, dried silkworm chrysalis meal, ferrous sulfate, magnesium chloride, aspartic acid.

According to an embodiment of the invention, wherein, the preparation process of the bacteria cellulose is：Strain is added into kind In sub- culture medium, 20h is cultivated at 28 DEG C, is inoculated into fermentation medium, 24h is cultivated at 25 DEG C, decantation, produces bacterium Cellulose；Wherein, the strain is to acetify bacillus；It is glucose 2.5%, sweet potato that the seed culture medium, which includes percentage by weight, Starch 1.3%, tryptone 0.7%, disodium hydrogen phosphate 0.32%, citric acid 0.18%, magnesium sulfate 0.11%, cobalt chloride 0.06%；Institute It is glucose 2.8%, dusty yeast 1.2%, dried silkworm chrysalis meal 0.7%, tryptone 0.5%, phosphorus to state fermentation medium and include percentage by weight Sour disodium hydrogen 0.27%, lactic acid 0.15%, acetic acid 0.06%, ferrous sulfate 0.03%, magnesium chloride 0.05%, aspartic acid 0.02%.

According to an embodiment of the invention, wherein, the described plant water extract amount of attaching most importance to part is compared for 1:2.2:1.5: 2.8:0.6 Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine section wood and the water extract of peppermint.

According to an embodiment of the invention, wherein, the preparation process of the plant water extract is：By Chinese ash Then skin, shaddock ped, gynostemma pentaphylla, nine section wood and peppermint are dried and crushed, be placed in 10-30 times first through 500W microwave treatment 5-15min In distilled water, the microwave treatment 20-60min under 500W power, room temperature, decantation are cooled to, then are placed in weight in 10-30 times of distilled water Above-mentioned microwave treatment step is carried out again 3-5 times, filtering, merging filtrate, and moisture content is evaporated to paste, produce.

According to an embodiment of the invention, described medical use anti-infection Wound dressing, as prepared by following raw material, press Parts by weight are calculated as：Bacteria cellulose 5.7-13.2 parts, PAHy 0.8-2.4 parts, polyvinyl alcohol 0.4-1.2 parts, polypropylene Sour sodium 0.4-0.8 parts, tragacanth 2.5-5.6 parts, allantoin 0.8-2.2 parts, galacturonic acid 0.7-1.4 parts, tumer how hydrogen Quinone 0.05-0.13 parts, Kaempferol glucuronide 0.14-0.22 parts, plant water extract 1.3-3 parts, polyoxyethylene polyoxy Propylene ether block copolymers 2.8-6.2 parts, polyethyleneglycol diglycidylether 0.6-3.5 parts, glycerine 18-27 parts, distilled water 30- 38 parts.

Therefore, the present invention provides the preparation method of medical use anti-infection Wound dressing, including following preparation process：

1）Bacteria cellulose is placed in and adds 70-85 DEG C of 10-20 times of parts by weight, in 0.5-1.2wt.% NaOH solution, with 50-200r/min rotating speeds stir 50-150min, filter and rinse to neutrality, freeze-drying, produce pretreatment bacteria cellulose；

2）Polyvinyl alcohol, tragacanth, allantoin, galacturonic acid are added in distilled water, are heated to 60-80 DEG C of stirring 30- 80min, obtain solution M；

3）By Sodium Polyacrylate, tumer, how quinhydrones, PAHy and Kaempferol glucuronide are added in glycerine, heating To 40-55 DEG C of stirring 20-50min, solution N is obtained；

4）Solution M is added in reactor, adds step 1 while stirring）Obtained pretreatment bacteria cellulose, stir 15- 30min, plant water extract and polyoxyethylene poly-oxygen propylene aether block copolymer are then added, stir 30-50min；

5）Then by step 3）Obtained solution N is added in reactor, and after being uniformly mixed, it is sweet to add polyethylene glycol diglycidyl Oily ether, it is well mixed, pours into mould, be stirred 2-4h at 50-65 DEG C, be cooled to room temperature, produce.

Beneficial effects of the present invention are：

The antibacterial anti-infection of Medical wounded surface dressing prepared by the present invention is good, to pseudomonas aeruginosa, staphylococcus aureus and The inhibiting rate of Candida albicans respectively reach 96.5%, 97.3% and 97.8% and its more than, there is broad spectrum antibacterial；And this is applied The healing cycle of material was controlled within one week, promoted to be cured significant effect, while regenerate the smooth exquisiteness of skin surface, no scar after healing Produce, outstanding to the healing ability of the surface of a wound, healing effect is good, is applicable to a variety of wounds such as wound, burn, ulcer and controls Treat.

Embodiment

With reference to embodiment, the present invention is described in further detail.Following examples are used to illustrate the present invention, but not For limiting the scope of the present invention.

Embodiment 1

Medical use anti-infection Wound dressing, as prepared by following raw material, it is calculated as by weight：5 parts of bacteria cellulose, PAHy 0.5 part, 0.1 part of polyvinyl alcohol, 0.3 part of Sodium Polyacrylate, 2 parts of tragacanth, 0.6 part of allantoin, 0.4 part of galacturonic acid, vinegar Sour first how 0.03 part of quinhydrones, 0.1 part of Kaempferol glucuronide, 1.2 parts of plant water extract, polyoxyethylene poly-oxygen propylene aether 2.4 parts of block copolymer, 0.5 part of polyethyleneglycol diglycidylether, 15 parts of glycerine, 26 parts of distilled water.

The preparation process of the bacteria cellulose is：Strain is added in seed culture medium, 10h is cultivated at 20 DEG C, then It is seeded in fermentation medium, 10h is cultivated at 20 DEG C, decantation, produces bacteria cellulose；Wherein, the strain is glucose Bacillus；It is glucose 2.5%, sweet potato starch 1.3%, tryptone 0.7%, phosphoric acid that the seed culture medium, which includes percentage by weight, Disodium hydrogen 0.32%, citric acid 0.18%, magnesium sulfate 0.11%, cobalt chloride 0.06%；The fermentation medium includes percentage by weight For glucose 2.8%, dusty yeast 1.2%, dried silkworm chrysalis meal 0.7%, tryptone 0.5%, disodium hydrogen phosphate 0.27%, lactic acid 0.15%, second Acid 0.06%, ferrous sulfate 0.03%, magnesium chloride 0.05%, aspartic acid 0.02%.

Described vegetation water extract weight part is compared for 1:2.6:1.8:2.1:0.4 Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine Section wood and the water extract of peppermint；The preparation process of the plant water extract is：By Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine sections Then wood and peppermint are dried and crushed, be placed in 10 times of distilled water, the microwave under 500W power first through 500W microwave treatment 5min 20min is handled, is cooled to room temperature, decantation, then is placed in 10 times of distilled water and repeats above-mentioned microwave treatment step 3 times, filtering, Merging filtrate, and moisture content is evaporated to paste, produce.

The preparation method of above-mentioned medical use anti-infection Wound dressing, including following preparation process：

1）Bacteria cellulose is placed in and adds 70 DEG C of 10 times of parts by weight, in 0.5wt.% NaOH solution, with 50r/min rotating speeds 50min is stirred, filters and rinses to neutrality, freeze-drying, produce pretreatment bacteria cellulose；

2）Polyvinyl alcohol, tragacanth, allantoin, galacturonic acid are added in distilled water, 60 DEG C of stirring 30min is heated to, obtains Solution M；

3）By Sodium Polyacrylate, tumer, how quinhydrones, PAHy and Kaempferol glucuronide are added in glycerine, heating To 40 DEG C of stirring 20min, solution N is obtained；

4）Solution M is added in reactor, adds step 1 while stirring）Obtained pretreatment bacteria cellulose, 15min is stirred, Then plant water extract and polyoxyethylene poly-oxygen propylene aether block copolymer are added, stirs 30min；

5）Then by step 3）Obtained solution N is added in reactor, and after being uniformly mixed, it is sweet to add polyethylene glycol diglycidyl Oily ether, it is well mixed, pours into mould, be stirred 2h at 50 DEG C, be cooled to room temperature, produce.

Embodiment 2

Medical use anti-infection Wound dressing, as prepared by following raw material, it is calculated as by weight：15 parts of bacteria cellulose, poly- asparagus fern acyl 3 parts of hydrazine, 1.4 parts of polyvinyl alcohol, 1 part of Sodium Polyacrylate, 6 parts of tragacanth, 2.5 parts of allantoin, 1.8 parts of galacturonic acid, acetic acid First how 0.15 part of quinhydrones, 0.28 part of Kaempferol glucuronide, 3.2 parts of plant water extract, polyoxyethylene poly-oxygen propylene aether 7.3 parts of block copolymer, 4 parts of polyethyleneglycol diglycidylether, 28 parts of glycerine, 40 parts of distilled water.

The preparation process of the bacteria cellulose is：Strain is added in seed culture medium, 24h is cultivated at 30 DEG C, then It is seeded in fermentation medium, 24h is cultivated at 30 DEG C, decantation, produces bacteria cellulose；Wherein, the strain is that onion is false Monad；It is glucose 2.5%, sweet potato starch 1.3%, tryptone 0.7%, phosphorus that the seed culture medium, which includes percentage by weight, Sour disodium hydrogen 0.32%, citric acid 0.18%, magnesium sulfate 0.11%, cobalt chloride 0.06%；The fermentation medium includes weight percent Than for glucose 2.8%, dusty yeast 1.2%, dried silkworm chrysalis meal 0.7%, tryptone 0.5%, disodium hydrogen phosphate 0.27%, lactic acid 0.15%, Acetic acid 0.06%, ferrous sulfate 0.03%, magnesium chloride 0.05%, aspartic acid 0.02%.

Described vegetation water extract weight part is compared for 1:1.5:1:2.4:1.6 Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine sections Wood and the water extract of peppermint；The preparation process of the plant water extract is：By Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine section wood With peppermint first through 500W microwave treatment 15min, then dry and crush, be placed in 30 times of distilled water, the microwave under 500W power 60min is handled, is cooled to room temperature, decantation, then is placed in 30 times of distilled water and repeats above-mentioned microwave treatment step 5 times, filtering, Merging filtrate, and moisture content is evaporated to paste, produce.

The preparation method of above-mentioned medical use anti-infection Wound dressing, including following preparation process：

1）Bacteria cellulose is placed in and adds 85 DEG C of 20 times of parts by weight, in 1.2wt.% NaOH solution, with 200r/min rotating speeds 150min is stirred, filters and rinses to neutrality, freeze-drying, produce pretreatment bacteria cellulose；

2）Polyvinyl alcohol, tragacanth, allantoin, galacturonic acid are added in distilled water, 80 DEG C of stirring 80min is heated to, obtains Solution M；

3）By Sodium Polyacrylate, tumer, how quinhydrones, PAHy and Kaempferol glucuronide are added in glycerine, heating To 55 DEG C of stirring 50min, solution N is obtained；

4）Solution M is added in reactor, adds step 1 while stirring）Obtained pretreatment bacteria cellulose, 30min is stirred, Then plant water extract and polyoxyethylene poly-oxygen propylene aether block copolymer are added, stirs 50min；

5）Then by step 3）Obtained solution N is added in reactor, and after being uniformly mixed, it is sweet to add polyethylene glycol diglycidyl Oily ether, it is well mixed, pours into mould, be stirred 4h at 65 DEG C, be cooled to room temperature, produce.

Embodiment 3

Medical use anti-infection Wound dressing, as prepared by following raw material, it is calculated as by weight：5.7 parts of bacteria cellulose, poly- asparagus fern acyl 0.8 part of hydrazine, 0.4 part of polyvinyl alcohol, 0.4 part of Sodium Polyacrylate, 2.5 parts of tragacanth, 0.8 part of allantoin, galacturonic acid 0.7 Part, tumer how 0.05 part of quinhydrones, 0.14 part of Kaempferol glucuronide, 1.3 parts of plant water extract, polyoxyethylene polyoxy 2.8 parts of propylene ether block copolymers, 0.6 part of polyethyleneglycol diglycidylether, 18 parts of glycerine, 30 parts of distilled water.

The preparation process of the bacteria cellulose is：Strain is added in seed culture medium, 18h is cultivated at 25 DEG C, then It is seeded in fermentation medium, 24h is cultivated at 25 DEG C, decantation, produces bacteria cellulose；Wherein, the strain is to acetify bar Bacterium；It is glucose 2.5%, sweet potato starch 1.3%, tryptone 0.7%, phosphoric acid hydrogen that the seed culture medium, which includes percentage by weight, Disodium 0.32%, citric acid 0.18%, magnesium sulfate 0.11%, cobalt chloride 0.06%；The fermentation medium is comprising percentage by weight Glucose 2.8%, dusty yeast 1.2%, dried silkworm chrysalis meal 0.7%, tryptone 0.5%, disodium hydrogen phosphate 0.27%, lactic acid 0.15%, acetic acid 0.06%th, ferrous sulfate 0.03%, magnesium chloride 0.05%, aspartic acid 0.02%.

Described vegetation water extract weight part is compared for 1:2.2:1.5:2.8:0.6 Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine Section wood and the water extract of peppermint；The preparation process of the plant water extract is：By Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine sections Then wood and peppermint are dried and crushed, be placed in 20 times of distilled water first through 500W microwave treatment 10min, micro- under 500W power Ripple handles 40min, is cooled to room temperature, decantation, then is placed in 25 times of distilled water and repeats above-mentioned microwave treatment step 5 times, mistake Filter, merging filtrate, and moisture content is evaporated to paste, produce.

The preparation method of above-mentioned medical use anti-infection Wound dressing, including following preparation process：

1）Bacteria cellulose is placed in and adds 78 DEG C of 18 times of parts by weight, in 0.8wt.% NaOH solution, with 150r/min rotating speeds 70min is stirred, filters and rinses to neutrality, freeze-drying, produce pretreatment bacteria cellulose；

2）Polyvinyl alcohol, tragacanth, allantoin, galacturonic acid are added in distilled water, 70 DEG C of stirring 65min is heated to, obtains Solution M；

3）By Sodium Polyacrylate, tumer, how quinhydrones, PAHy and Kaempferol glucuronide are added in glycerine, heating To 48 DEG C of stirring 40min, solution N is obtained；

4）Solution M is added in reactor, adds step 1 while stirring）Obtained pretreatment bacteria cellulose, 25min is stirred, Then plant water extract and polyoxyethylene poly-oxygen propylene aether block copolymer are added, stirs 40min；

5）Then by step 3）Obtained solution N is added in reactor, and after being uniformly mixed, it is sweet to add polyethylene glycol diglycidyl Oily ether, it is well mixed, pours into mould, be stirred 4h at 58 DEG C, be cooled to room temperature, produce.

Embodiment 4

Medical use anti-infection Wound dressing, as prepared by following raw material, it is calculated as by weight：13.2 parts of bacteria cellulose, poly- asparagus fern 2.4 parts of hydrazides, 1.2 parts of polyvinyl alcohol, 0.8 part of Sodium Polyacrylate, 5.6 parts of tragacanth, 2.2 parts of allantoin, galacturonic acid 1.4 Part, tumer how 0.13 part of quinhydrones, 0.22 part of Kaempferol glucuronide, 3 parts of plant water extract, polyoxyethylene polyoxy third 6.2 parts of alkene ether block copolymers, 3.5 parts of polyethyleneglycol diglycidylether, 27 parts of glycerine, 38 parts of distilled water.

The preparation process of the bacteria cellulose is：Strain is added in seed culture medium, 18h is cultivated at 28 DEG C, then It is seeded in fermentation medium, 16h is cultivated at 25 DEG C, decantation, produces bacteria cellulose；Wherein, the strain is that onion is false Monad；It is glucose 2.5%, sweet potato starch 1.3%, tryptone 0.7%, phosphorus that the seed culture medium, which includes percentage by weight, Sour disodium hydrogen 0.32%, citric acid 0.18%, magnesium sulfate 0.11%, cobalt chloride 0.06%；The fermentation medium includes weight percent Than for glucose 2.8%, dusty yeast 1.2%, dried silkworm chrysalis meal 0.7%, tryptone 0.5%, disodium hydrogen phosphate 0.27%, lactic acid 0.15%, Acetic acid 0.06%, ferrous sulfate 0.03%, magnesium chloride 0.05%, aspartic acid 0.02%.

Described vegetation water extract weight part is compared for 1:2.2:1.5:2.8:0.6 Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine Section wood and the water extract of peppermint；The preparation process of the plant water extract is：By Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine sections Then wood and peppermint are dried and crushed, be placed in 25 times of distilled water first through 500W microwave treatment 10min, micro- under 500W power Ripple handles 40min, is cooled to room temperature, decantation, then is placed in 20 times of distilled water and repeats above-mentioned microwave treatment step 4 times, mistake Filter, merging filtrate, and moisture content is evaporated to paste, produce.

The preparation method of above-mentioned medical use anti-infection Wound dressing, including following preparation process：

1）Bacteria cellulose is placed in and adds 75 DEG C of 18 times of parts by weight, in 0.8wt.% NaOH solution, with 180r/min rotating speeds 75min is stirred, filters and rinses to neutrality, freeze-drying, produce pretreatment bacteria cellulose；

2）Polyvinyl alcohol, tragacanth, allantoin, galacturonic acid are added in distilled water, 75 DEG C of stirring 55min is heated to, obtains Solution M；

3）By Sodium Polyacrylate, tumer, how quinhydrones, PAHy and Kaempferol glucuronide are added in glycerine, heating To 40-55 DEG C of stirring 45min, solution N is obtained；

4）Solution M is added in reactor, adds step 1 while stirring）Obtained pretreatment bacteria cellulose, 25min is stirred, Then plant water extract and polyoxyethylene poly-oxygen propylene aether block copolymer are added, stirs 50min；

5）Then by step 3）Obtained solution N is added in reactor, and after being uniformly mixed, it is sweet to add polyethylene glycol diglycidyl Oily ether, it is well mixed, pours into mould, be stirred 4h at 58 DEG C, be cooled to room temperature, produce.

Comparative example 1

Difference in this comparative example with embodiment 1 is：Polyoxyethylene poly-oxygen propylene aether block copolymer, allantoin are not added How quinhydrones, other raw materials and its content and preparation method are the same as embodiment 1 with tumer.

Comparative example 2

Difference in this comparative example with embodiment 1 is：PAHy, Kaempferol glucuronide and its phase are not added Step is closed, other raw materials and its content and preparation method are the same as embodiment 1.

Comparative example 3

Difference in this comparative example with embodiment 1 is：Nine sections wood and Chinese ash bark are not added in the plant water extract, Simultaneously the bacteria cellulose using grape saccharobacillus by routine culture medium and cultural method prepared by and must, other raw materials And its content and preparation method are the same as embodiment 1.

Carried out below for the Medical wounded surface casting product prepared by various embodiments above and comparative example according to professional standard Correlated performance detection, its specific testing result are：

	Bacteriostasis rate/%（Pseudomonas aeruginosa）	Bacteriostasis rate/%（Staphylococcus aureus）	Bacteriostasis rate/%（Candida albicans）	Healing cycle/day	Healing effect
						Embodiment 1	96.5	97.3	97.8	5-7	Without scar
Embodiment 2	98.2	98.7	99.0	5-6	Without scar
						Embodiment 3	99.1	99.4	99.2	4-5	Without scar
Embodiment 4	99.5	99.7	99.7	3-4	Without scar
						Comparative example 1	87.5	88.3	86.4	8-9	Without scar
Comparative example 2	78.2	84.7	82.5	8-10	Slight scar
						Comparative example 3	71.6	74.5	67.6	9-12	Moderate scar

The antibacterial anti-infection of the Medical wounded surface dressing prepared from above test result, the present invention is good, to P. aeruginosa The inhibiting rate of bacterium, staphylococcus aureus and Candida albicans respectively reach 96.5%, 97.3% and 97.8% and its more than, have Broad spectrum antibacterial；And the healing cycle of this dressing was controlled within one week, promote to be cured significant effect, while skin is regenerated after healing The smooth exquisiteness in surface, no scar produce, and outstanding to the healing ability of the surface of a wound, healing effect is good, be applicable to wound, burn, A variety of wound treatments such as ulcer.

Claims (6)

1. medical use anti-infection Wound dressing, it is characterised in that as prepared by following raw material, be calculated as by weight：Bacteria cellulose 5-15 parts, PAHy 0.5-3 parts, polyvinyl alcohol 0.1-1.4 parts, Sodium Polyacrylate 0.3-1 parts, tragacanth 2-6 parts, allantois Plain 0.6-2.5 parts, galacturonic acid 0.4-1.8 parts, tumer how quinhydrones 0.03-0.15 parts, Kaempferol glucuronide 0.1-0.28 parts, plant water extract 1.2-3.2 parts, polyoxyethylene poly-oxygen propylene aether block copolymer 2.4-7.3 parts, poly- second two Alcohol diglycidyl ether 0.5-4 parts, glycerine 15-28 parts, distilled water 26-40 parts；

The plant water extract is Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine section wood and the water extract of peppermint；

The preparation process of the bacteria cellulose is：Strain is added in seed culture medium, 10-24h is cultivated at 20-30 DEG C, Inoculate into fermentation medium, 10-24h is cultivated at 20-30 DEG C, decantation, produces bacteria cellulose；Wherein, the strain To acetify bacillus, Pseudomonas cepacia or grape saccharobacillus；The seed culture medium includes glucose, sweet potato starch, tryptose Peptone, disodium hydrogen phosphate, citric acid, magnesium sulfate, cobalt chloride；The fermentation medium include glucose, dusty yeast, tryptone, Disodium hydrogen phosphate, lactic acid, acetic acid, dried silkworm chrysalis meal, ferrous sulfate, magnesium chloride, aspartic acid.

2. medical use anti-infection Wound dressing according to claim 1, it is characterised in that the preparation step of the bacteria cellulose Suddenly it is：Strain is added in seed culture medium, 20h is cultivated at 28 DEG C, inoculates into fermentation medium, cultivated at 25 DEG C 24h, decantation, produce bacteria cellulose；Wherein, the strain is to acetify bacillus；The seed culture medium includes percentage by weight For glucose 2.5%, sweet potato starch 1.3%, tryptone 0.7%, disodium hydrogen phosphate 0.32%, citric acid 0.18%, magnesium sulfate 0.11%th, cobalt chloride 0.06%；It is glucose 2.8%, dusty yeast 1.2%, dried silkworm chrysalis meal that the fermentation medium, which includes percentage by weight, 0.7%th, tryptone 0.5%, disodium hydrogen phosphate 0.27%, lactic acid 0.15%, acetic acid 0.06%, ferrous sulfate 0.03%, magnesium chloride 0.05%th, aspartic acid 0.02%.

3. medical use anti-infection Wound dressing according to claim 1, it is characterised in that the plant water extract amount of attaching most importance to Part is than being 1:2.2:1.5:2.8:0.6 Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine section wood and the water extract of peppermint.

4. medical use anti-infection Wound dressing according to claim 1, it is characterised in that the preparation of the plant water extract Step is：By Chinese ash bark, shaddock ped, gynostemma pentaphylla, nine section wood and peppermint first through 500W microwave treatment 5-15min, then dry simultaneously Crush, be placed in 10-30 times of distilled water, the microwave treatment 20-60min under 500W power, be cooled to room temperature, decantation, then be placed in Repeat above-mentioned microwave treatment step in 10-30 times of distilled water 3-5 times, filtering, merging filtrate, and moisture content is evaporated to paste, Produce.

5. according to any described medical use anti-infection Wound dressings of claim 1-4, it is characterised in that made by following raw material It is standby, it is calculated as by weight：It is bacteria cellulose 5.7-13.2 parts, PAHy 0.8-2.4 parts, polyvinyl alcohol 0.4-1.2 parts, poly- PAA 0.4-0.8 parts, tragacanth 2.5-5.6 parts, allantoin 0.8-2.2 parts, galacturonic acid 0.7-1.4 parts, tumer How quinhydrones 0.05-0.13 parts, Kaempferol glucuronide 0.14-0.22 parts, plant water extract 1.3-3 parts, polyoxyethylene Polyoxypropylene ether block copolymers 2.8-6.2 parts, polyethyleneglycol diglycidylether 0.6-3.5 parts, glycerine 18-27 parts, distillation Water 30-38 parts.

6. the preparation method of the medical use anti-infection Wound dressing as described in claim 1-5 is any, it is characterised in that including as follows Preparation process：

1）Bacteria cellulose is placed in and adds 70-85 DEG C of 10-20 times of parts by weight, in 0.5-1.2wt.% NaOH solution, with 50-200r/min rotating speeds stir 50-150min, filter and rinse to neutrality, freeze-drying, produce pretreatment bacteria cellulose；

2）Polyvinyl alcohol, tragacanth, allantoin, galacturonic acid are added in distilled water, are heated to 60-80 DEG C of stirring 30- 80min, obtain solution M；

3）By Sodium Polyacrylate, tumer, how quinhydrones, PAHy and Kaempferol glucuronide are added in glycerine, heating To 40-55 DEG C of stirring 20-50min, solution N is obtained；

4）Solution M is added in reactor, adds step 1 while stirring）Obtained pretreatment bacteria cellulose, stir 15- 30min, plant water extract and polyoxyethylene poly-oxygen propylene aether block copolymer are then added, stir 30-50min；

5）Then by step 3）Obtained solution N is added in reactor, and after being uniformly mixed, it is sweet to add polyethylene glycol diglycidyl Oily ether, it is well mixed, pours into mould, be stirred 2-4h at 50-65 DEG C, be cooled to room temperature, produce.