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CN107137783A - A kind of preparation method of the composite regenerated fiber repair materials of zeins - Google Patents

A kind of preparation method of the composite regenerated fiber repair materials of zeins Download PDF

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Publication number
CN107137783A
CN107137783A CN201710404904.4A CN201710404904A CN107137783A CN 107137783 A CN107137783 A CN 107137783A CN 201710404904 A CN201710404904 A CN 201710404904A CN 107137783 A CN107137783 A CN 107137783A
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CN
China
Prior art keywords
solution
zeins
regenerated
repair materials
preparation
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Pending
Application number
CN201710404904.4A
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Chinese (zh)
Inventor
王吉鹏
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Hefei Create Technology Co Ltd
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Hefei Create Technology Co Ltd
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Publication date
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Priority to CN201710404904.4A priority Critical patent/CN107137783A/en
Publication of CN107137783A publication Critical patent/CN107137783A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/58—Materials at least partially resorbable by the body
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0015—Electro-spinning characterised by the initial state of the material
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
    • D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
    • A61L2300/602—Type of release, e.g. controlled, sustained, slow

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mechanical Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Artificial Filaments (AREA)
  • Materials For Medical Uses (AREA)

Abstract

A kind of preparation method of the composite regenerated fiber repair materials of zeins, belongs to tissue renovation material preparing technical field, including：Repair materials are made in the preparation of zein solution, the preparation of regenerated cellulose spinning solution, electrostatic spinning.Repair materials produced by the present invention have good anti-microbial property, mechanical performance, tissue repair performance and drug attachment.

Description

A kind of preparation method of the composite regenerated fiber repair materials of zeins
Technical field
The invention belongs to tissue renovation material preparing technical field, more particularly to a kind of composite regenerated fibre of zeins Tie up the preparation method of repair materials.
Background technology
Cellulose as natural biopolymer, with biodegradability, the physics of biocompatibility and uniqueness, Chemistry and mechanical performance, it has also become the study hotspot in new bio medical material field.And prior art is fine using regeneration mostly Dimension element is blended with other materials, and freeze-drying prepares composite membrane, using be combined made from the method film uniformity, stability compared with Difference, and tissue repairing ability and drug attachment need further raising.
The content of the invention
In order to solve problems of the prior art, the invention provides it is a kind of with good tissue repairing ability and The preparation method of the composite regenerated fiber repair materials of zeins of drug attachment.
The present invention uses following technical scheme：
A kind of preparation method of the composite regenerated fiber repair materials of zeins, comprises the following steps：
Step one：Zeins is dissolved in glacial acetic acid, mass fraction is prepared molten for 24% zeins 3h is stirred under liquid, normal temperature magnetic force, 24 hours froth breakings are stood；
Step 2：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1: 50, silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, use deionization Water is cleaned, and degumming fibroin is obtained after air-drying；
Step 3：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, it is 5wt.%'s that concentration, which is made, CaCl2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin molten Liquid, drying and forming-film, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk egg by film with deionized water at room temperature Tunica albuginea is secondary under room temperature state to be dissolved in formic acid, and bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning Liquid；
Step 4：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 5：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed to obtain regenerated fiber Plain spinning solution；
Step 6：By zein solution and regenerated cellulose spinning solution in mass ratio 4:19 are well mixed, and use Ultrasonic oscillation disperses to it, magnetic agitation 1h under the conditions of 50 DEG C, then continues to stir 8h at room temperature, obtains viscous solution It is placed in the medical needle tubing being connected with high-pressure installation, repair materials is made by electrostatic spinning, high-pressure installation voltage is 20kV, is spun Silk flow 2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, is dried in vacuo at 40 DEG C.
It is preferred that, the capacity of the medical needle tubing described in step 6 is 20ml.
It is preferred that, the syringe needle of the medical needle tubing described in step 6 is fixed at electrostatic spinning receiver board 20cm.
It is preferred that, the syringe needle internal diameter of the medical needle tubing described in step 6 is 0.41mm.
The beneficial effects of the present invention are：
1) zeins is as a kind of wide material sources, the natural polymer raw material of relative low price, with fine Biocompatibility and degradability, and with hydrophobicity, be adapted to contain medicine, prevent soluble agents to be quickly lost in, Be conducive to medicament slow release.
2) fibroin albumen has good antibiotic property, biocompatibility and tissue repairing ability.
3) cellulose has good biocompatibility, degradability and excellent mechanical performance.
4) cellulose can improve the mechanical performance of material, and fibroin albumen can improve materials microstructure repair ability.
5) material prepared using electrostatic spinning technique, is further increased the porosity of material, is conducive to the attached of medicine .
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of preparation method of the composite regenerated fiber repair materials of zeins, comprises the following steps：
Step one：Zeins is dissolved in glacial acetic acid, mass fraction is prepared molten for 24% zeins 3h is stirred under liquid, normal temperature magnetic force, 24 hours froth breakings are stood；
Step 2：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1: 50, silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, use deionization Water is cleaned, and degumming fibroin is obtained after air-drying；
Step 3：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, it is 5wt.%'s that concentration, which is made, CaCl2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin molten Liquid, drying and forming-film, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk egg by film with deionized water at room temperature Tunica albuginea is secondary under room temperature state to be dissolved in formic acid, and bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning Liquid；
Step 4：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 5：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed to obtain regenerated fiber Plain spinning solution；
Step 6：By zein solution and regenerated cellulose spinning solution in mass ratio 4:19 are well mixed, and use Ultrasonic oscillation disperses to it, magnetic agitation 1h under the conditions of 50 DEG C, then continues to stir 8h at room temperature, obtains viscous solution It is placed in the medical needle tubing being connected with high-pressure installation, repair materials is made by electrostatic spinning, high-pressure installation voltage is 20kV, is spun Silk flow 2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, is dried in vacuo at 40 DEG C.
The capacity of medical needle tubing described in step 6 is 20ml, and the syringe needle of described medical needle tubing is fixed on apart from electrostatic At spinning receiver board 20cm, syringe needle internal diameter is 0.41mm.

Claims (4)

1. a kind of preparation method of the composite regenerated fiber repair materials of zeins, it is characterised in that comprise the following steps：
Step one：Zeins is dissolved in glacial acetic acid, the zein solution that mass fraction is 24% is prepared, often 3h is stirred under warm magnetic force, 24 hours froth breakings are stood；
Step 2：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1:50, remove Silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, be washed with deionized water Only, degumming fibroin is obtained after air-drying；
Step 3：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, the CaCl that concentration is 5wt.% is made2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin solution, room The lower drying and forming-film of temperature, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk fibroin film by film with deionized water Secondary under room temperature state to be dissolved in formic acid, bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning solution；
Step 4：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 5：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed that regenerated cellulose is spun Silk liquid；
Step 6：By zein solution and regenerated cellulose spinning solution in mass ratio 4:19 are well mixed, and use ultrasound Ripple concussion disperses to it, magnetic agitation 1h under the conditions of 50 DEG C, then continues to stir 8h at room temperature, obtains viscous solution and is placed in In the medical needle tubing being connected with high-pressure installation, repair materials are made by electrostatic spinning, high-pressure installation voltage is 20kV, spinning stream 2ml/h is measured, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, is dried in vacuo at 40 DEG C.
2. a kind of preparation method of the composite regenerated fiber repair materials of zeins according to claim 1, it is special Levy and be：The capacity of medical needle tubing described in step 6 is 20ml.
3. a kind of preparation method of the composite regenerated fiber repair materials of zeins according to claim 1, it is special Levy and be：The syringe needle of medical needle tubing described in step 6 is fixed at electrostatic spinning receiver board 20cm.
4. a kind of preparation method of the composite regenerated fiber repair materials of zeins according to claim 1, it is special Levy and be：The syringe needle internal diameter of medical needle tubing described in step 6 is 0.41mm.
CN201710404904.4A 2017-06-01 2017-06-01 A kind of preparation method of the composite regenerated fiber repair materials of zeins Pending CN107137783A (en)

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Application Number Priority Date Filing Date Title
CN201710404904.4A CN107137783A (en) 2017-06-01 2017-06-01 A kind of preparation method of the composite regenerated fiber repair materials of zeins

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117582560A (en) * 2023-10-09 2024-02-23 常州市智态生创科技有限公司 Repairing film for preventing intrauterine adhesion and preparation method and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103341209A (en) * 2013-07-08 2013-10-09 苏州大学 Silk fibroin nanofiber membrane and preparation method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103341209A (en) * 2013-07-08 2013-10-09 苏州大学 Silk fibroin nanofiber membrane and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117582560A (en) * 2023-10-09 2024-02-23 常州市智态生创科技有限公司 Repairing film for preventing intrauterine adhesion and preparation method and application thereof

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Application publication date: 20170908