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CN107185041A - A kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide - Google Patents

A kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide Download PDF

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Publication number
CN107185041A
CN107185041A CN201710404509.6A CN201710404509A CN107185041A CN 107185041 A CN107185041 A CN 107185041A CN 201710404509 A CN201710404509 A CN 201710404509A CN 107185041 A CN107185041 A CN 107185041A
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China
Prior art keywords
solution
collagen peptide
preparation
regenerated
repair materials
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CN201710404509.6A
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Inventor
王吉鹏
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Hefei Create Technology Co Ltd
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Hefei Create Technology Co Ltd
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Priority to CN201710404509.6A priority Critical patent/CN107185041A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/58—Materials at least partially resorbable by the body
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
    • D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
    • D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
    • D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
    • D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/23—Carbohydrates
    • A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Artificial Filaments (AREA)
  • Materials For Medical Uses (AREA)

Abstract

A kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide, belongs to tissue renovation material preparing technical field, including：Repair materials are made in the preparation of cod collagen peptide, the preparation of bamboo nanofiber solution, the preparation of regenerated cellulose spinning solution, electrostatic spinning.Tissue renovation material produced by the present invention has good anti-microbial property, mechanical performance, tissue repair performance and drug attachment.

Description

A kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide
Technical field
The invention belongs to tissue renovation material preparing technical field, more particularly to a kind of composite regenerated fibre of cod collagen peptide Tie up the preparation method of repair materials.
Background technology
Cellulose as natural biopolymer, with biodegradability, the physics of biocompatibility and uniqueness, Chemistry and mechanical performance, it has also become the study hotspot in new bio medical material field.And prior art is fine using regeneration mostly Dimension element is blended with other materials, and freeze-drying prepares composite membrane, using be combined made from the method film uniformity, stability compared with Difference, and tissue repairing ability and drug attachment need further raising.
The content of the invention
In order to solve problems of the prior art, the invention provides it is a kind of with good tissue repairing ability and The preparation method of the composite regenerated fiber repair materials of cod collagen peptide of drug attachment.
The present invention uses following technical scheme：
A kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide, comprises the following steps：
Step one：Cod collagen is taken, the collagen aqueous solution of mass concentration 1% is configured to, collagen egg is adjusted White water solution pH value is 8.0, adds the alkali protease of mass percent 1%, enzyme digestion reaction is carried out in 50 DEG C of water-bath After 60min, boiling water goes out enzyme 10min, is cooled to room temperature with 8000r/min centrifugal treating 15min, takes supernatant rotary evaporation to concentrate To the half of original volume, cod collagen peptide extract is obtained；
Step 2：20g bamboo fibres are weighed, are put into the NaOH solution that 400g, mass fraction are 17.5%, and be placed into Temperature is in 100 DEG C of thermostat water bath, 60min is boiled in boiling, then bamboo fibre is cleaned to neutrality repeatedly with deionized water, is placed in 6h is dried in 70 DEG C of baking ovens, crushed after being dried crosses 80 mesh sieves into coarse powder, obtains bamboo powder；
Step 3：Take 16gLiCl to put into equipped with 40 DEG C, the container of 184g dimethyl acetamides, stirred in constant temperature magnetic force Mixing makes LiCl fully dissolve in the presence of device, is configured to the solvent that mass fraction is 8%, takes made from solvent 100g and step 2 Bamboo powder 10g, mixes 3h in 60 DEG C of thermostat water baths, is placed in Ultrasonic cell smash, power output 800W's Under the conditions of ultrasonically treated 15min, obtain bamboo nanofiber solution；
Step 4：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1: 50, silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, use deionization Water is cleaned, and degumming fibroin is obtained after air-drying；
Step 5：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, it is 5wt.%'s that concentration, which is made, CaCl2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin molten Liquid, drying and forming-film, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk egg by film with deionized water at room temperature Tunica albuginea is secondary under room temperature state to be dissolved in formic acid, and bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning Liquid；
Step 6：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 7：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed to obtain regenerated fiber Plain spinning solution；
Step 8：By cod collagen peptide extract, bamboo nanofiber solution, regenerated cellulose spinning solution in mass ratio 3: 4:19 are well mixed, and it are disperseed using ultrasonic oscillation, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir at room temperature 8h is mixed, viscous solution is obtained and is placed in the medical needle tubing being connected with high-pressure installation, repair materials, high pressure are made by electrostatic spinning Device voltage is 20kV, spinning flow 2ml/h, and obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, 40 It is dried in vacuo at DEG C.
It is preferred that, the capacity of the medical needle tubing described in step 8 is 20ml.
It is preferred that, the syringe needle of the medical needle tubing described in step 8 is fixed at electrostatic spinning receiver board 20cm.
It is preferred that, the syringe needle internal diameter of the medical needle tubing described in step 8 is 0.41mm.
The beneficial effects of the present invention are：
1) cod collagen peptide is a kind of material to skin with Pleiotropic Protections, can repair skin injury, is mended The collagen filled in skin.
2) fibroin albumen and bamboo nanofiber have good antibiotic property and biocompatibility.
3) cellulose has good biocompatibility, degradability and excellent mechanical performance.
4) cellulose can improve the mechanical performance of material, and fibroin albumen, cod collagen peptide can improve the group of material Knit repair ability.
5) bamboo nanofiber, and the material prepared using electrostatic spinning technique are added, the hole of material is further increased Rate, is conducive to the attachment of medicine.
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
Step one：Cod collagen is taken, the collagen aqueous solution of mass concentration 1% is configured to, collagen egg is adjusted White water solution pH value is 8.0, adds the alkali protease of mass percent 1%, enzyme digestion reaction is carried out in 50 DEG C of water-bath After 60min, boiling water goes out enzyme 10min, is cooled to room temperature with 8000r/min centrifugal treating 15min, takes supernatant rotary evaporation to concentrate To the half of original volume, cod collagen peptide extract is obtained；
Step 2：20g bamboo fibres are weighed, are put into the NaOH solution that 400g, mass fraction are 17.5%, and be placed into Temperature is in 100 DEG C of thermostat water bath, 60min is boiled in boiling, then bamboo fibre is cleaned to neutrality repeatedly with deionized water, is placed in 6h is dried in 70 DEG C of baking ovens, crushed after being dried crosses 80 mesh sieves into coarse powder, obtains bamboo powder；
Step 3：Take 16gLiCl to put into equipped with 40 DEG C, the container of 184g dimethyl acetamides, stirred in constant temperature magnetic force Mixing makes LiCl fully dissolve in the presence of device, is configured to the solvent that mass fraction is 8%, takes made from solvent 100g and step 2 Bamboo powder 10g, mixes 3h in 60 DEG C of thermostat water baths, is placed in Ultrasonic cell smash, power output 800W's Under the conditions of ultrasonically treated 15min, obtain bamboo nanofiber solution；
Step 4：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1: 50, silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, use deionization Water is cleaned, and degumming fibroin is obtained after air-drying；
Step 5：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, it is 5wt.%'s that concentration, which is made, CaCl2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin molten Liquid, drying and forming-film, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk egg by film with deionized water at room temperature Tunica albuginea is secondary under room temperature state to be dissolved in formic acid, and bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning Liquid；
Step 6：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 7：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed to obtain regenerated fiber Plain spinning solution；
Step 8：By cod collagen peptide extract, bamboo nanofiber solution, regenerated cellulose spinning solution in mass ratio 3: 4:19 are well mixed, and it are disperseed using ultrasonic oscillation, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir at room temperature 8h is mixed, viscous solution is obtained and is placed in the medical needle tubing being connected with high-pressure installation, repair materials, high pressure are made by electrostatic spinning Device voltage is 20kV, spinning flow 2ml/h, and obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, 40 It is dried in vacuo at DEG C.
The capacity of medical needle tubing described in step 8 is 20ml, and the syringe needle of described medical needle tubing is fixed on apart from electrostatic At spinning receiver board 20cm, syringe needle internal diameter is 0.41mm.

Claims (4)

1. a kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide, it is characterised in that comprise the following steps：
Step one：Cod collagen is taken, the collagen aqueous solution of mass concentration 1% is configured to, collagen water is adjusted Solution ph is 8.0, adds the alkali protease of mass percent 1%, and enzyme digestion reaction 60min is carried out in 50 DEG C of water-bath Afterwards, boiling water goes out enzyme 10min, is cooled to room temperature with 8000r/min centrifugal treating 15min, takes supernatant rotary evaporation to be concentrated into original The half of volume, obtains cod collagen peptide extract；
Step 2：20g bamboo fibres are weighed, are put into the NaOH solution that 400g, mass fraction are 17.5%, and be placed into temperature For in 100 DEG C of thermostat water baths, 60min is boiled in boiling, then bamboo fibre is cleaned to neutrality repeatedly with deionized water, is placed in 70 DEG C 6h is dried in baking oven, crushed after being dried crosses 80 mesh sieves into coarse powder, obtains bamboo powder；
Step 3：16gLiCl is taken to put into equipped with 40 DEG C, the container of 184g dimethyl acetamides, in constant temperature blender with magnetic force In the presence of LiCl is fully dissolved, be configured to mass fraction be 8% solvent, take bamboo powder made from solvent 100g and step 2 10g, mixes 3h in 60 DEG C of thermostat water baths, is placed in Ultrasonic cell smash, in power output 800W condition Under ultrasonically treated 15min, obtain bamboo nanofiber solution；
Step 4：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1:50, remove Silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, be washed with deionized water Only, degumming fibroin is obtained after air-drying；
Step 5：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, the CaCl that concentration is 5wt.% is made2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin solution, room The lower drying and forming-film of temperature, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk fibroin film by film with deionized water Secondary under room temperature state to be dissolved in formic acid, bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning solution；
Step 6：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 7：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed that regenerated cellulose is spun Silk liquid；
Step 8：By cod collagen peptide extract, bamboo nanofiber solution, regenerated cellulose spinning solution in mass ratio 3:4:19 It is well mixed, it is disperseed using ultrasonic oscillation, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir at room temperature 8h, obtains viscous solution and is placed in the medical needle tubing being connected with high-pressure installation, and repair materials are made by electrostatic spinning, and height press-fits Voltage is put for 20kV, spinning flow 2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, at 40 DEG C Lower vacuum drying.
2. a kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide according to claim 1, it is special Levy and be：The capacity of medical needle tubing described in step 8 is 20ml.
3. a kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide according to claim 1, it is special Levy and be：The syringe needle of medical needle tubing described in step 8 is fixed at electrostatic spinning receiver board 20cm.
4. a kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide according to claim 1, it is special Levy and be：The syringe needle internal diameter of medical needle tubing described in step 8 is 0.41mm.
CN201710404509.6A 2017-06-01 2017-06-01 A kind of preparation method of the composite regenerated fiber repair materials of cod collagen peptide Pending CN107185041A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108619486A (en) * 2018-08-13 2018-10-09 常同喜 It is a kind of to treat dermopathic ointment and preparation method thereof
CN108914368A (en) * 2018-07-24 2018-11-30 高昕文 A kind of preparation method of facial mask fabric nonwoven cloth

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103173892A (en) * 2013-04-03 2013-06-26 北京石油化工学院 Preparation method of nano bamboo fiber composite material
CN103341209A (en) * 2013-07-08 2013-10-09 苏州大学 Silk fibroin nanofiber membrane and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103173892A (en) * 2013-04-03 2013-06-26 北京石油化工学院 Preparation method of nano bamboo fiber composite material
CN103341209A (en) * 2013-07-08 2013-10-09 苏州大学 Silk fibroin nanofiber membrane and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108914368A (en) * 2018-07-24 2018-11-30 高昕文 A kind of preparation method of facial mask fabric nonwoven cloth
CN108619486A (en) * 2018-08-13 2018-10-09 常同喜 It is a kind of to treat dermopathic ointment and preparation method thereof
CN108619486B (en) * 2018-08-13 2021-12-24 常同喜 Ointment for treating skin diseases and preparation method thereof

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