CN106982642A - A kind of asexual reproduction method of Qinling Mountains purple bergenia herb - Google Patents
A kind of asexual reproduction method of Qinling Mountains purple bergenia herb Download PDFInfo
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- CN106982642A CN106982642A CN201710382175.7A CN201710382175A CN106982642A CN 106982642 A CN106982642 A CN 106982642A CN 201710382175 A CN201710382175 A CN 201710382175A CN 106982642 A CN106982642 A CN 106982642A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
A kind of asexual reproduction method of Qinling Mountains purple bergenia herb, the sprout that height exceedes 5cm take root cultivations, and sprout retains 0.1~1cm rhizome base portion and repairing and cuts flat with whole, and rhizome base portion is with after 0.1~2.0 mg L 1IBA immersions, 15~50s, then plants in mixed-matrix.Highly it is less than 5cm sprout, remaines on primitive root stem, carries out rhizome tloig-cutlage, the length of rhizome fringe bar is repaiied and is cut to 1~6 cm, 3~July or cuttage in 9~November in mixed-matrix.Mixed-matrix is made up of perlite, turfy soil and humus, first pours permeable before planting, and moisturizing, keeps illuminance in the 1x of 2000 1x~3000, daily 10~14 h light application times, temperature of shed is 20~28 DEG C, imposes rich water quality management, cultivation 2~3 months.The method operation possibility of the present invention is strong, and management is simple, and cost is low, makes it possible large area artificial cultivation Qinling Mountains purple bergenia herb.
Description
Technical field：
The invention belongs to field of biology, it is related to Qinling Mountains purple bergenia herb, specifically a kind of Qinling Mountains purple bergenia herb is asexual numerous
Grow method.
Background technology：
Qinling Mountains purple bergenia herb (Bergenia scopulosa T.P.Wang) is Saxifragaceae Bergenia perennial herb,
The platymiscium whole world has 10 kinds, and China produces 7 kinds, and its root-like stock of Qinling Mountains purple bergenia herb is referred to as interwined dragon in Shaanxi Mei County and Qinling Mountain Areas
Seven, one of 3 endemic species of China are not only, and be the kind of this category most original.Being distributed in domestic wild species more Shaanxi, Sichuan and
The southeast of Gansu, enters people's winter, fallen leaves of withering the original producton location more, but it is relatively early to bloom, and flower pink colour, ornamental value is high.Herb contains
Have Bergenin, rhizome content of dispersion is higher, by《Chinese book on Chinese herbal medicine》With《Shaanxi Chinese herbal medicine》Recorded, be that Shaanxi is among the people conventional
Medicine is puckery, bitter, flat；With good efficacy to suffering from a deficiency of the kidney, the solid intestines of inducing astrigency, dehumidify Li Shui, effect of promoting blood circulation；It is treating acute and chronic enterogastritis, floating
Swollen, the favus of the scalp, mange etc., are the specific drugs for treating respiratory disease.In recent years, scholars were adapted to its Flowering Characteristics, pollination
Property and propagating system are analyzed and researched, it is believed that it has Self- hybriddegeneration phenomenon, therefore Natural seed setting rate is extremely low, seed germination rate
It is not high, it is slow-growing.
Seminar established Qinling Mountains purple bergenia herb fast breeding technique system in 2012, and took the lead in realizing standard metaplasia at home
Production, protects wild resource, the high quality seedling produced has carried out demonstration plant in Xinjiang, Henan, Fujian, zhejiang and other places, receives
Good social benefit and ecological benefits is arrived.Fee of material, labour cost continue surging in recent years so that tissue culture produces taking for seedling
With also remaining high, in order to save production cost, the purpose of high quality seedling can be provided again, seminar is carried out with regard to Qinling Mountains purple bergenia herb
Line culture, and the plant division sprout number of annual tissue-cultured seedling has been counted, taken root using plant division sprout cultivating and with bud rhizome cuttage
Deng vegetative manner, make it possible large area artificial cultivation and industrialization development.
The content of the invention：
It is an object of the invention to provide a kind of asexual reproduction method of Qinling Mountains purple bergenia herb, described this Qinling Mountains purple bergenia herb
Asexual reproduction method to solve the technical problem of wild Qinling Mountains purple bergenia herb low reproduction rate in the prior art, again reduce tissue culture production
The too high present situation of middle expense, makes it possible large area artificial cultivation and industrialization development.
The invention provides a kind of asexual reproduction method of Qinling Mountains purple bergenia herb, including the step of selection sprout, if
Described sprout highly more than 5cm, then retain 0.1~1cm rhizome base portion and repairing cut flat with it is whole, rhizome base portion with 0.1~
2.0mg·L-1IBA is soaked after 15~50s, then is planted and cultivated in mixed-matrix；If the height of sprout is less than 5cm, protect
Stay on primitive root stem, carry out rhizome tloig-cutlage, the length of rhizome fringe bar is repaiied and is cut to 1~6cm, 3~July or 9~11
Moon cuttage is in mixed-matrix；Described mixed-matrix is made up of perlite, turfy soil and humus, first pours permeable before planting, so
Shed closing moisturizing is taken with plastic sheeting afterwards, illuminance is kept in the 1x of 2000 1x~3000, daily 10~14h light application times,
Temperature of shed is 20~28 DEG C, imposes rich water quality management, is cultivated 2~3 months..
Further, sprout rhizome base portion can retain the interval any lengths of 0.1~1cm；IBA can for 0.1~
2.0mg·L-1Interval any concentration；IBA soak times can be any time interval 15~50s.
Further, the length of rhizome fringe bar, which can be repaiied, is cut to the interval any lengths of 1~6cm；Can in 3~July or
Person carries out cuttage in interval any month in 9~November.
Further, mixed-matrix is with water before use, first irrigated, after plantation after pour 1500x carbendazim, kind after delay
Parch and irrigating after before seedling moisturizing, slow seedling, and extend illumination to cultivate healthy and strong seedling, it is between twice of rich water plus one time to pour fertile principle
Clear water, its EC is controlled between 250~500um/cm, applies within 5~7 days a bactericide.
Further, described fertilizer is made up of the precious power Fenghe ammonium calcium and magnesium of fertilizer, the matter of described precious power Fenghe ammonium calcium and magnesium
Measure as 7:3.
Further, in described shed illuminance may remain in the 1x of 2000 1x~3000 it is interval between it is any strong
Degree；Light application time may remain in 10~14h it is interval between any time, temperature may remain in 20~128 DEG C of intervals it
Between any temperature.
Further, when seedling root forms soil ball, when highly for 10cm, it is implanted into the small alms bowl that bore is 8cm and carries out
Cultivate again.
The present invention is compared with prior art, and its technological progress is positive and obvious.Take root in cultivation, rooting rate highest can
Up to 100%.In cottage breeding, survival rate reaches as high as 90%.The present invention is taken root or rhizome tloig-cutlage using sprout cultivating
Qinling Mountains purple bergenia herb is cultivated Deng vegetative manner, seedling cost is reduced, turning into large area artificial cultivation and industrialization development can
Can, protect wild resource.
Brief description of the drawings：
Fig. 1 is the photo of the annual tissue-cultured seedling sprout sum of Qinling Mountains purple bergenia herb 10.
Fig. 2 is that Qinling Mountains purple bergenia herb sprout is taken root the photo of situation.
Fig. 3 is the photo of Qinling Mountains purple bergenia herb rhizome.
Fig. 4 is the photo of Qinling Mountains purple bergenia herb rhizome cuttage.
Fig. 5 is the photo of Qinling Mountains purple bergenia herb rhizome cutting survival seedling.
Embodiment：
During following embodiment statistics, every kind of processing investigates 20, and every kind of processing is repeated 3 times.Statistics sprout is taken root
Rate, rhizome cuttage survival rate.Sprout rooting rate=strain number/inoculation number of taking root, rhizome cuttage survival rate=survive number/cuttage number.
Statistical analysis is carried out to data using Excel2003 and the data handling systems of DPS 14.5.Using completely random list
Factorial experiments statistic law and LSD methods carry out variance analysis and Multiple range test (α=0.05)
Embodiment 1
Sprout is taken root cultivation
1.1 gradient design
Sprout take root cultivation when, using NAA and IBA processing, be set to 0.1,0.2,0.5,1.0,1.5,2.0 count 6 kinds it is dense
Spend gradient；Soak time is set to six gradients of 10s, 15s, 20s, 30s, 40s, 50s；Sprout base portion is repaiied and is cut to 0.1,0.2,
0.5th, 0.8, five kinds of length of 1.0cm.
1.2 condition of culture
Sprout take root cultivation when, in greenhouse carry out, pour permeable after operation, then taken with plastic sheeting shed closing guarantor
Wet, capping straw screen or mat or sunshade net shelter from heat or light cooling in good time, and holding illuminance is in the 1x of 2000 1x~3000, daily 10~14h illumination
Time, temperature of shed is 20~28 DEG C.
1.3 sprout cultivating
The influence that the different growth hormone of table 1 and its concentration are taken root to sprout
* data are mean+/-standard error (n=3) in table, and the letters different with after column of figure represent 0.05 level error
Different conspicuousness (P ﹤ 0.05), similarly hereinafter.
Sprout highly more than 5cm take root cultivations, and reservation 1cm base portion and repairing cuts flat with whole, is grown with various concentrations
After element processing 20s, then plant in the mixed-matrix prepared, impose rich water quality management, rooting rate is counted after one month, difference swashs
The influence significant difference (table 1) that element and its concentration are taken root to sprout.NAA or IBA processing is either used, with hormone concentration
Rise, rooting rate is also with raising, when concentration is 1.0mgL-1When, rooting rate reaches highest, respectively 65%, 75%, it
Auxin concentration is raised afterwards, there is inhibition to taking root on the contrary, and rooting rate declines.Screen IBA1.0mgL-1As optimization process
Concentration, is compared, significant difference with other processing.
Using 1.0mgL-1IBA soaks sprout base portion, and soak time is set to 10s, 15s, 20s, 30s, 40s, 50s six
Individual gradient.As a result show, soak time influences notable to rooting rate, in short-term speed leaching, rooting rate is not high, as soak time is prolonged
Long, rooting rate is quickly improved, when soak time is 30s, and rooting rate reaches highest 95% (table 2), is compared with other processing, difference shows
Write.Comprehensive hormone kind, concentration and soak time, filter out 1.0mgL-1IBA immersions 30s is optimum treatment mode.
The influence that the different soak times of table 2 are taken root to sprout
Using 1.0mgL-1IBA soaks sprout base portion 30s, sprout base portion is repaiied and is cut to 0.1,0.2,0.5,0.8,1.0cm five
Plant length.As a result show, sprout base portion rhizome is longer, and rooting rate is higher, when length is 0.5cm, rooting rate reaches highest
100%, compared with two kinds of processing less than 0.5cm, significant difference (table 3).Examined according to result of the test and saving root medicinal material
Consider, the optimal reservation length of sprout base portion is 0.5cm.
The influence that the different sprout base lengths of table 3 are taken root to sprout
The rhizome cuttage of embodiment 2
Highly it is less than 5cm sprout, individually cuts down to be not easy to take root and survive, preferably remain on primitive root stem, carries out root
Stem cuttage, can just be survived.
2.1 experimental design
During rhizome cuttage, repair and be cut to six kinds of length of 1cm, 2cm, 3cm, 4cm, 5cm, 6cm；Cutting time is set to 1~12
The different months such as moon.
2.2 condition of culture
During rhizome cuttage, carried out in greenhouse, pour permeable after operation, shed closing moisturizing is then taken with plastic sheeting, is fitted
When capping straw screen or mat or sunshade net shelter from heat or light cooling, holding illuminance is in 2000~3000 1x or so, 10~14h light application times, in canopy
Temperature is 20~28 DEG C or so.
2.3 rhizome cuttages
Rhizome, which is repaiied, is cut to six kinds of length of 1cm, 2cm, 3cm, 4cm, 5cm, 6cm, it is found that when rhizome length is less than 4cm, for
Cuttage influence is significantly (table 4).When rhizome length be 1cm when, cuttage survival rate is only 15%, and when rhizome length reach 4cm and
During the above, cuttage survival rate can reach 100%, and how much this is closely related with rhizome accumulation nutrition.In order to reduce planting cost
Root yield is improved, 4cm is taken during rhizome cuttage.
Influence of the different rhizome lengths of table 4 to cuttage survival rate
When taking 4cm rhizome cuttages, season is different, and survival rate is also variant, and different month cuttages find (table 5), 3~July,
9~November, cuttage survival rate is compared, significant difference more than 90% with other months；And August part rainwater it is more when,
There is part putrefactive phenomenon in rhizome, and it is 85% to influence survival rate lower slightly；And during 2 months of December to next year, survival rate is even
55% is reduced to, this is low with temperature, rhizome hair new root has direct relation slowly.
Influence of the different month cuttages of table 5 to survival rate
The matrix of embodiment 3 and management
Qinling Mountains purple bergenia herb sprout culture of rootage and rhizome tloig-cutlage all use the import turf of good air permeability, and turf is long
2mm or so is spent, with perlite:Turfy soil:Humus=2:1:1 ratio is modulated into mixed-matrix, and 2d is irrigated before plantation, after kind
Pour 1500x carbendazim.Small water is mended after kind before slow seedling, where where benefit is done, was evaporated and be preferred with the same day；Parched after slow seedling
Irrigate, and healthy and strong seedling is cultivated in proper extension illumination.It is between twice of rich water plus a clear water to pour fertile principle, and its EC controls exist
Between 250~500um/cm, fertilizer presses Bao Lifeng：Ammonium calcium and magnesium=7:3 ratio mixing.5~7d applies a bactericide, anti-in time
Control root maggot.When maintenance was to 2 months, whether sprout or rhizome cuttage seeding, root has formed soil ball, is highly 10cm, this
When can be sold as finished product seedling.The rooted seedling of more than 2 months needs to be implanted into a diameter of 8cm of bore small alms bowl, in favor of
Late growing stage.
Conclusion:
Purple bergenia herb sprout culture of rootage or rhizome cuttage cost are low, manage relatively simple, material is thus formed pass through group
Next life antenatal period female parent seedling is trained, the later stage, by sprout culture of rootage or the production chain of rhizome cutting propagation, is extensive plant
Exploitation is planted there is provided guarantee and premise.
Claims (10)
1. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb, it is characterised in that：The step of including a selection sprout, if described
Sprout highly more than 5cm, then retain 0.1~1cm rhizome base portion and repairing and cut flat with whole, rhizome base portion is with 0.1~2.0 mg L- 1IBA is soaked after 15~50s, then is planted and cultivated in mixed-matrix；If the height of sprout is less than 5cm, primitive root is remained in
On stem, carry out rhizome tloig-cutlage, the length of rhizome fringe bar repaiied and is cut to 1~6 cm, in 3~July or cuttage in 9~November in
In mixed-matrix；Described mixed-matrix is made up of perlite, turfy soil and humus, is first poured permeable before planting, is then used plastics
Film takes shed closing moisturizing, keeps illuminance in the 1x of 2000 1x~3000, daily 10~14 h light application times, temperature of shed
For 20~28 DEG C, rich water quality management is imposed, is cultivated 2~3 months.
2. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 1, it is characterised in that：Sprout rhizome base portion
Optimal reservation length be 0.5cm.
3. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 1, it is characterised in that：Sprout rhizome base portion
It is 1.0 mg L with the IBA optium concentrations soaked-1。
4. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 1, it is characterised in that：Sprout rhizome base portion
The Best Times soaked with IBA are 30s.
5. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 1, it is characterised in that：Rhizome fringe bar is most
Jia Xiuqie length is 4 cm.
6. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 1, it is characterised in that：Described mixed base
In matter, the mass ratio of perlite, turfy soil and humus is 2:1:1.
7. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 1, it is characterised in that：Mixed-matrix is used
A few days ago, first irrigated with water, pour to parch after moisturizing before slow seedling, slow seedling after 1500x carbendazim, kind again after plantation and irrigate,
And extend illumination to cultivate healthy and strong seedling, it is between twice of rich water plus a clear water to pour fertile principle, and its EC is controlled 250~500
Between um/cm, a bactericide is applied within 5~7 days.
8. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 7, it is characterised in that：Described fertilizer by
Precious power Fenghe ammonium calcium and magnesium composition, the quality of described precious power Fenghe ammonium calcium and magnesium is 7:3.
9. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 1, it is characterised in that：In described shed
Optimal illuminance is 2500 1x, and daily optimum illumination time is 12 h, and optimum temperature is 25 DEG C.
10. a kind of asexual reproduction method of Qinling Mountains purple bergenia herb according to claim 1, it is characterised in that：When seedling root
Soil ball is formed, when highly for 10cm, is implanted into the small alms bowl that bore is 8cm and is cultivated again.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710382175.7A CN106982642B (en) | 2017-05-22 | 2017-05-22 | Asexual propagation method of Qinling mountain bergenia |
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| CN201710382175.7A CN106982642B (en) | 2017-05-22 | 2017-05-22 | Asexual propagation method of Qinling mountain bergenia |
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| CN102550411A (en) * | 2011-12-31 | 2012-07-11 | 四川省农业科学院园艺研究所 | Method for producing pre-basic seeds of potatoes |
| CN104255245A (en) * | 2014-09-15 | 2015-01-07 | 姚建成 | Fresh ginger stem root overwintered seedling breeding method |
| CN104641905A (en) * | 2015-02-13 | 2015-05-27 | 贵州百灵企业集团制药股份有限公司 | Standardized planting method of saxifraga stolonifera meerb |
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