CN106124405A - Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel measures system - Google Patents
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel measures system Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/19—Dichroism
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
- G01N21/554—Attenuated total reflection and using surface plasmons detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
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Abstract
The present invention relates to a kind of circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel and measure system.Including light source, the adjustable polarizer, One Dimension Periodic metallic channel structure, sample flow pond and spectrogrph；Described light source can produce continuous different wavelengths of light；The described adjustable polarizer for being modulated into the line polarized light of the apposing symmetric angle of polarization by incident non-polarized light；In described One Dimension Periodic metallic channel structure makes incident illumination be radiated at its groove and surface produces the chirality light field of local enhancing；Described sample flow pond is used for loading liquid tested chirality sample, and when described One Dimension Periodic metallic channel structure is placed in sample flow pond so that liquid tested chirality sample at the uniform velocity flow through One Dimension Periodic metallic channel body structure surface, it is simple to ensure signal stabilization during measurement；Described spectrogrph is for detecting the spectral signal after One Dimension Periodic metallic channel structure reflects.The present invention line polarized light incidence One Dimension Periodic metallic channel structure by twice apposing symmetric polarization angle, it is poor by the tested chirality sample reflected signal that absorbing light difference causes under twice incidence to detect, it is achieved detect the circular dichroism spectra of sample.
Description
Technical field
The present invention relates to Molecular Spectroscopy field, a kind of circle based on line polarized light incidence One Dimension Periodic metallic channel
Dichroscope spectral measurement system.
Background technology
Chirality is the key character in the structure of matter, i.e. have can not be overlapping Three-dimensional glass as enantiomer, they
Molecular formula is identical, but differently configured in space of its Atom or atomic radical, mirror image each other.A lot of biomolecule such as eggs
White matter, DNA, aminoacid etc. make them cannot overlap with the mirror image of oneself due to their space structure structural property, and we claim
They are chiral molecule.Direct and molecule the function of the space chirality feature of these molecules is closely bound up, the most chiral molecule
Chiral analysis most important.And the most effective technology of molecular chiral analysis at present is circular dichroism (circular
Dichroism, CD).Chiral molecule all has optical activity.As monochromatic left-handed (left-handed circularly
Polarized light, LCP) and dextrorotation (right-handed circularly polarized light, RCP)
When circularly polarized light is by a certain chirality sample, this sample is to the absorption (absorption, A) of left and right rounding polarized light no
With, this is called circular dichroism.Its difference DELTA A=Δ A (LCP)-Δ A (RCP) is referred to as circular dichroism spectra at different wavelengths.
Can be seen that the contrary left and right circularly polarized light of generation chirality realizing circular dichroism detection most critical.Current business
Circularly polarised wave in circular dichroism instrument is all to be produced by light ball modulator.Light ball modulator is mainly suitable saturating by one piece
Luminescent material (such as fused quartz or calcium fluoride etc.) is attached on piezoelectric transducer composition.Utilizing photoelastic effect, it can make linear polarization
Light becomes left circularly polarized light and the right-circularly polarized light of the higher-order of oscillation.So monochromatic by monochromator when synchrotron radiation line polarized light
After change, when entering the modulation head that light ball modulator is passed through in human sample room, reform into the left-handed and dextrorotation of the higher-order of oscillation (such as 50kHz)
Circularly polarized light, converges at sample center.If chirality sample, then the absorption to left and right rounding polarized light is different, causes left and right circle
The light transmissive light energy that polarizes is different, is just adopted by lock-in amplifier finally by after the amplification to faint transmission differential signal again
Collection is to stable circular dichroism signal.To different wave length, the CD value of sample is also different, step motor control monochromator carry out wavelength
Scanning, has just obtained the CD spectrum of this sample.
Can be seen that traditional circular dichroism instrument has certain deficiency to limit it and extensively applies.First, it is to tested
Sample there are certain requirements, and usually needs to put into solution example in the sample cell of Centimeter Level length and measures, so sample is had one
The requirement of individual minimum flow, therefore it cannot realize the measurement to few chirality sample；Second, due to the light ball modulator one used
Secondary a specific wavelength can only be played modulating action, therefore incident illumination has to pass through monochromator and carries out length scanning, surveys every time
Measure the circular dichroism value under a specific wavelength, so cause the circular dichroism testing time under whole wide spectrum to increase.3rd, system
Optics used by complexity is a lot, and therefore commercialization instrument price is expensive, and follow-up maintenance cost is the highest.Exactly because it is traditional
There is disadvantages mentioned above in circular dichroism instrument, the detection method always scientific circles of the simplest sensitiveer circular dichroism grind
The focus studied carefully.
Along with the development of this micro-nano technology technology, the processing of the micro-nano structure of sub-wavelength becomes possibility.Recent years, science
Family finds that special metal micro-nanostructure can strengthen circular dichroism and absorb, and improves its sensitivity.Its mechanism is when incidence
The micro-nano structure of light stimulus metal, can produce a kind of referred to as " local surface plasma resonance " (localized surface
Plasmonic resonance, LSPR) phenomenon, the electromagnetic wave in metal surface can be made highly constrained on surface, thus real
Show the electromagnetic field at near-field region greatly to strengthen.This Electromagnetic enhancement effect can increase the mutual of incident illumination and sensing sample
Effect, thus realize the enhancing to circular dichroism and detect.It not the most that the electric field strengthened result in circular dichroism
Enhancing, but determined this hands by the enhancing strengthening " photolytic activity " (the optical chirality) of light field that electric field causes
Property molecule the enhancing of circular dichroism." photolytic activity " has chirality also can be on the occasion of there being negative value, and chiral molecule is to having not
Absorb difference with photoactive light, be to lead chiral molecule the basic reason of circular dichroism occurs.Left and right circularly polarized light possesses phase
Anti-photolytic activity, therefore can be used to carry out circular dichroism spectra detection.Research shows, special micro-nano structure is at plasma resonance
Wavelength produces in can be implemented in territory, near field and strengthens chirality electromagnetic field, namely possesses photolytic activity, therefore may be used for strengthening circle two
Color spectrum.Such as, EHendry in 2010 et al. (Hendry, Euan, et al. " Ultrasensitive detection
and characterization of biomolecules using superchiral fields." Nature
Nanotechnology 5.11 (2010): 783-787.) find that the metallic channel of two-dimentional fylfot of plane is at left and right entelechy
Produce the chirality light field strengthened on the surface of structure under the incidence changed, therefore can be as a kind of scheme strengthening circular dichroism.
2015, Tullius et al. (Tullius, Ryan, et al. " " Superchiral " spectroscopy:
detection of protein higher order hierarchical structure with chiral
plasmonic nanostructures." Journal of the American Chemical Society 137.26
Therefore and left and right (2015): 8380-8383.) made the starfish appearance metal structure of 2D, possessed chirality due to structure itself,
Circularly polarised wave interacts difference, utilizes this principle to realize the detection of high order spatial foldable structure of chiral protein.
Mousavi in 2016 et al. (Mousavi, S., et al. " Large Enhancement of Circular
Dichroism Using an Embossed Chiral Metamaterial." arXiv preprint arXiv:
1604.05244 (2016) .) to propose hyporelief second wavelength metallic structure in breach semicircle permissible under the incidence of circularly polarized light
Produce the chirality electromagnetic field strengthened, it is achieved that strengthen circular dichroism spectra sensing.Can be seen that through well-designed sub-wavelength micro-nano
Metal structure under the excitation of incident illumination due to surface plasma resonance effect, the photolytic activity of local is strengthened, and therefore has
Hope and realize strengthening circular dichroism detection.But, these schemes above-mentioned typically require the chirality micro-nano of a pair specular each other
Structure, the difference because left and right circularly polarized light is interacted by the micro-nano structure possessing chirality, need a pair each other " enantiomer "
Micro-nano structure is such as with left-hand screw metallic channel and the right-hand screw metallic channel structure of size.When two have opposite-handed micro-nano gold
When genus structure has the identical chirality sample being detected, owing to the selectivity of chirality sample causes the circular dichroism light of two structures
The skew of spectrum is different, therefore can be used to the sensing of the chirality sample additional to surface.Although this concept feasible, but still
Having two deficiencies the most serious: first, this scheme is higher to sample processing request, because originally being drawn by chiral molecule
The difference signal itself of absorption risen is the faintest, and this scheme needs to measure the sample mirror image of two specular each other respectively,
So must assure that the pattern Striking symmetry of two samples and size are exactly the same, otherwise it is likely to appearance by sample processing by mistake
The measurement error that difference causes has flooded conceivable signal originally；Second, the scheme of this enhancing circular dichroism still needs
Use left and right circularly polarized light, still must measure by traditional circular dichroism system, therefore do not drop on cost
Low, the detection strengthening circular dichroism is simply achieved by special micro-nano structure.
It is previously noted that the basic condition producing circular dichroism is the chirality electromagnetic field producing and having difference " photolytic activity ".
Traditional circular dichroism instrument is through the manipulator of costliness and can realize the incident beam modulated different photoactive for possessing
Left and right circularly polarized light.Along with finding to it is found that line polarized light can also be by some micro-nano plasmas to the research of micro-nano structure
Resonant structure realizes realizing electromagnetic wave in the modulation of local area electric field and possesses photolytic activity, and this is to realize circle two by linear polarization light
Chromatograph detects the possibility provided.
Sch ferling in 2012 et al. (Sch ferling, Martin, Xinghui Yin, and Harald
Giessen. "Formation of chiral fields in a symmetric environment." Optics
Express 20.24 (2012): 26326-26336.) even if research finds the square micro-nano metal that line polarized light is incident on
Sheet can also produce the photolytic activity of enhancing near four angles, but that they chirality symbols of possessing differ namely have and just have
Negative value, not having clean photolytic activity the most on the whole increases.Subsequently, their (Scha ferling, Martin, et al. in 2014
"Helical plasmonic nanostructures as prototypical chiral near-field sources."
Acs Photonics 1.6 (2014): 530-537.) again proposition line polarized light irradiate three-dimensional helical structure and can realize
Perfect chirality electromagnetic field in spiraltron, this structure can potential be applied to strengthen circular dichroism sensing.But, but
Being that three-dimensional micro-nano structure processing technique is complicated proposes stern challenge to current manufacturing process, is difficulty with.Recently, Tian,
Xiaorui et al. (Tian, Xiaorui, Yurui Fang, and Mengtao Sun. " Formation of
enhanced uniform chiral fields in symmetric dimer nanostructures." Scientific
Reports 5 (2015) .) show a pair metal dimer centre under the irradiation of line polarized light by numerical simulation study
Internal field in gap can greatly strengthen, and becomes with gap under certain angle of inclination in incident polarization direction, electricity in gap
Magnetic field possesses photolytic activity, and produces a photoactive chirality and can be adjusted by incident light polarization direction.Although
What regional area had obtained strengthening there is photoactive chirality electromagnetic field, but due to the dimeric size limitation of metal,
Such as corner, other regions away from gap still there will be contrary photoactive electromagnetic field.So, if increased with the application of this structure
Strong circular dichroism, then these opposite-handed electromagnetic fields coexisted can reduce the overall performance of structure.
Summary can be seen that the internal field that (1) is produced by micro-nano metal structure support surface plasma resonance effect increases
Local light increased activity that is strong thus that cause can realize strengthening circular dichroism detection in principle.But at present by by micro-nano
Metal structure realizes strengthening the overwhelming majority in the scheme that circular dichroism detects and must also utilize left and right circularly polarized light respectively and sample
Condition interaction detectable signal, namely will be by traditional circular dichroism instrument, so the scheme cost of this circular dichroism spectra depends on
So must not be reduced.(2) the chirality electric field of enhancing can be produced in local by linear polarization excitation micro-nano metal structure, and
The photolytic activity of chirality electric field can be adjusted by line of incidence polarization direction.Although owing to the surrounding of micro-nano structure also can coexist
Contrary chirality electric field, can reduce the performance of entirety to a certain extent, but, utilize linear polarization to realize strengthening circular dichroism light
Spectrum detects useful huge applications and is worth, and is worth us to go to explore further, because this scheme is without relying on expensive traditional round
Dichroscope spectrometer can be greatly lowered the cost of current Chiral Pharmaceutical Analysis, and by micro-nano structure surface plasma resonance
Enhancement effect, can improve sensing sensitivity and realize the detection of the analysis to denier sample.
Summary of the invention
It is an object of the invention to provide a kind of circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel to survey
Amount system, surface plasma body resonant vibration that this system is supported by exciting micro-nano metal structure produce the local electromagnetic field strengthened and
Possess the photolytic activity of enhancing, and realize inside and outside metallic channel by adjusting the relative angle of line of incidence plane of polarization and metallic channel
The change of the photolytic activity chirality of electric field, produces the similar elliptical polarization electromagnetic field left oval, right strengthened at micro-nano structure surface, from
And realize the circular dichroism detection of chiral molecule.
For achieving the above object, the technical scheme is that one is based on line polarized light incidence One Dimension Periodic metallic channel
Circular dichroism measure system, including light source, the adjustable polarizer, One Dimension Periodic metallic channel structure, sample flow pond and spectrum
Instrument；Described light source can produce continuous different wavelengths of light；The described adjustable polarizer is for being modulated into phase by incident non-polarized light
The line polarized light of the antisymmetry angle of polarization；Described One Dimension Periodic metallic channel structure makes incident illumination be radiated at, and its groove is interior and surface produces
The chirality light field that local strengthens；Described sample flow pond is used for loading liquid tested chirality sample, and at described One Dimension Periodic gold
Belong to groove structure when being placed in sample flow pond so that liquid tested chirality sample at the uniform velocity flow through One Dimension Periodic metallic channel structural table
Face, it is simple to ensure signal stabilization during measurement；Described spectrogrph is for detecting the spectrum after One Dimension Periodic metallic channel structure reflects
Signal；
The light of light source outgoing, after the line polarized light that the adjustable polarizer is modulated into the apposing symmetric angle of polarization, is incident to described one respectively
In dimension cycle metallic channel structure, and in the chirality light field of the different chirality of One Dimension Periodic metallic channel structure generation, due to one-dimensional
Tested chirality sample in cycle metallic channel structure has different absorptions to the light of different chiral so that the apposing symmetric angle of polarization
The reflection produced after line polarized light incidence is different, by producing after the line polarized light incidence of the spectrometer collection apposing symmetric angle of polarization
Reflectance spectrum difference signal can characterize the circular dichroism signal of sample.
In an embodiment of the present invention, described light source is the wide range continuous light source that can produce continuous different wavelengths of light.
In an embodiment of the present invention, the line polarized light of the described apposing symmetric angle of polarization is i.e. relative to One Dimension Periodic metallic channel
Knot constitute+45 ° with-45 ° ,-30 ° with the line polarized light of+30 ° or-60 ° with+60 °.
In an embodiment of the present invention, described One Dimension Periodic metallic channel structure is support surface plasma resonance effect
Metallic channel.
In an embodiment of the present invention, the material that described One Dimension Periodic metallic channel structure uses is support surface plasma
The noble metal of resonance effect, including gold, silver, aluminum.
In an embodiment of the present invention, the size of described One Dimension Periodic metallic channel structure is sub-wavelength magnitude, i.e. less than light
Source outgoing wavelength.
In an embodiment of the present invention, described One Dimension Periodic metallic channel structure is sized to according to light source outgoing wave progress
Row sum-equal matrix.
In an embodiment of the present invention, described One Dimension Periodic metallic channel structure is for being completely independent structure or being machined in substrate
On.
In an embodiment of the present invention, described sample flow pond is that closing is capable of circulation, and it uses peristaltic pump liquid to circulate
System make the surface of One Dimension Periodic metallic channel structure at the uniform velocity flow through described tested chirality sample.
Compared to prior art, the method have the advantages that
1. present invention firstly provides and utilize line polarized light to realize the circular dichroism detection of chiral sample by micro-nano structure；Send out
Bright scheme need not first will enter with the expensive circular polarization polarizer (such as light ball modulator) as traditional round dichroscope spectra system
Penetrating light modulation is that circularly polarized light interacts with sample again, and obtains the light of different chirality only by change incident polarization angle
Field and sample effect, it is possible to obtain the circular dichroism signal of sample, measuring method is novel, measures process operation simple；
The device comprised in scheme the most disclosed by the invention is general, and the processing of metallic channel senser element is simple, and therefore complete machine cost is very
Low, there is the biggest using value.
Accompanying drawing explanation
Fig. 1 is that the principle of present invention circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel measurement system is shown
It is intended to.
Fig. 2 is the One Dimension Periodic metallic channel structural representation of embodiment one.
Fig. 3 is that in embodiment one, One Dimension Periodic metallic channel structure is becoming+45 ° with-45 ° of line polarized lights 14 ° tiltedly relative to groove
Reflectance spectrum under Ru She.
Fig. 4 is the average light activity of electromagnetic field in embodiment one metallic channel, and whole packing space (comprise metallic channel and
Packing space high for 1500nm above) electromagnetic field average light activity.
Fig. 5 is to fill after different chirality sample+45 ° and the difference of the incident reflectance spectrums of-45 ° of linear polarizations in embodiment one, also
The i.e. circular dichroism signal of sample.
Fig. 6 is the One Dimension Periodic metallic channel structural representation of embodiment two.
Fig. 7 is that in embodiment two, One Dimension Periodic metallic channel structure is becoming+45 ° and-45 ° of lower line polarized lights 14 ° relative to groove
Reflectance spectrum under oblique incidence；
Fig. 8 is the average light activity of electromagnetic field in embodiment two metallic channel, and whole packing space (comprises metallic channel and above
The packing space that 1500nm is high) electromagnetic field average light activity.
Fig. 9 is to fill after different chirality sample+45 ° and the difference of the incident reflectance spectrums of-45 ° of linear polarizations in embodiment two, also
The i.e. circular dichroism signal of sample.
Detailed description of the invention
Below in conjunction with the accompanying drawings, technical scheme is specifically described.
A kind of based on line polarized light incidence One Dimension Periodic metallic channel the circular dichroism of the present invention measures system, including light
Source, the adjustable polarizer, One Dimension Periodic metallic channel structure, sample flow pond and spectrogrph；Described light source can produce continuous difference
Wavelength light；The described adjustable polarizer for being modulated into the line polarized light of the apposing symmetric angle of polarization by incident non-polarized light；Described
In One Dimension Periodic metallic channel structure makes incident illumination be radiated at its groove and surface produces the chirality light field of local enhancing；Described sample
Flow cell is used for loading liquid tested chirality sample, and when described One Dimension Periodic metallic channel structure is placed in sample flow pond,
Make liquid tested chirality sample at the uniform velocity flow through One Dimension Periodic metallic channel body structure surface, it is simple to during measurement ensure signal stabilization；
Described spectrogrph is for detecting the spectral signal after One Dimension Periodic metallic channel structure reflects；
The light of light source outgoing, after the line polarized light that the adjustable polarizer is modulated into the apposing symmetric angle of polarization, is incident to described one respectively
In dimension cycle metallic channel structure, and in the chirality light field of the different chirality of One Dimension Periodic metallic channel structure generation, due to one-dimensional
Tested chirality sample in cycle metallic channel structure has different absorptions to the light of different chiral so that the apposing symmetric angle of polarization
The reflection produced after line polarized light incidence is different, by producing after the line polarized light incidence of the spectrometer collection apposing symmetric angle of polarization
Reflectance spectrum difference signal can characterize the circular dichroism signal of sample.
Described light source is the wide range continuous light source that can produce continuous different wavelengths of light.
The line polarized light of the described apposing symmetric angle of polarization i.e. relative to One Dimension Periodic metallic channel knot constitute+45 ° with-45 ° ,-
30 ° with the line polarized light of+30 ° or-60 ° with+60 °.
Described One Dimension Periodic metallic channel structure is the metallic channel of support surface plasma resonance effect.Described One Dimension Periodic
The noble metal that material is support surface plasma resonance effect that metallic channel structure uses, including gold, silver, aluminum.Described one-dimensional
The size of cycle metallic channel structure is sub-wavelength magnitude, i.e. less than light source outgoing wavelength.Described One Dimension Periodic metallic channel structure
It is sized to be adjusted according to light source outgoing wave length.Described One Dimension Periodic metallic channel structure is for being completely independent structure or being machined in
On substrate.
Described sample flow pond is that closing is capable of circulation, and it uses peristaltic pump fluid circulation system to make One Dimension Periodic metal
The surface of groove structure at the uniform velocity flow through described tested chirality sample.
It it is below the specific implementation process of the present invention.
Embodiment one
Fig. 1 is the systematic schematic diagram that reflective linear polarization incidence One Dimension Periodic metallic channel circular dichroism is measured.1 is incident illumination
Source, generally continuous print wide spectrum optical；2 is the adjustable linear polarization polarizer, for being adjusted to become with groove by incident non-polarized light+
45 ° and the line polarized light in-45 ° of directions；3 is One Dimension Periodic micro-nano metallic channel structure, is used for and incident illumination interaction generation etc.
Gas ions resonance also produces enhancing chirality electromagnetic field at body structure surface；4 is sample flow pond, for detected solution sample is immersed
Fully act on by the electromagnetic field of sample and micro-nano structure surface at metallic channel sample surfaces；5 is spectrogrph, is used for measuring reflection light
Spectrum.
The wide range continuous light that light source is sent out obtains directional light by whole bundle, and the linear polarization being then passed through an adjustable direction is polarized
Device obtains the line polarized light of specific direction, and line polarized light incides cycle metallic channel, and the light of reflection is adopted by opposite side spectrogrph
Collection.
Regulation polariser make the polarization direction of incident illumination and metallic channel in+45 °, due to plasma resonance effect and draw
The Phase delay effect risen can produce chirality electromagnetic field inside and outside metallic channel and that is to say possess photoactive electric field.When incident illumination is inclined
Shake be adjusted to additionally one side symmetry angle-45 ° time, then produce contrary photolytic activity chirality electromagnetic field inside and outside groove.Record twice not
With reflectance spectrum R (+45 °) under polarization angle and R (-45 °).
One Dimension Periodic metallic channel combines flowing water pond and realizes chirality solution example and flow through immersion groove at metallic channel surface-stable
Surface.During because tested chirality solution is immersed in metallic channel structure by stream pond, can produce near groove with incident illumination
Chirality electromagnetic field interacts, owing to right+45 ° of chirality sample absorbs not with the opposite-handed light field of generation under-45 ° of polarisation excitation
With, therefore+45 ° have difference with the reflective light intensity of-45 ° of polarized incident situations, namely Δ R=R (+45 °)-R (-45 °) is not
Zero, this difference signal Δ R is to be caused by the chirality of the sample filled, and that is to say the circular dichroism letter of the sample recorded
Number.
It it is the structural representation of One Dimension Periodic metallic channel in embodiment one shown in accompanying drawing 2.The groove cycle is 450nm, groove width
225nm, groove depth 400nm, trench bottom thickness 400nm, the material of groove is gold, and matrix is silicon dioxide；
See shown in accompanying drawing 3, for accompanying drawing 2 structure fill achirality sample air (assuming that refractive index is 1) time+45 ° with-45 °
Reflectance spectrum under linear polarization incidence.Owing to achirality sample is as broad as long to different chirality light, thus incident under symmetric polarized
Reflectance spectrum is just the same.
See shown in accompanying drawing 4, be structure shown in accompanying drawing 2 in the case of oblique incidence (oblique incidence angle is 14 °) ,+45 ° of polarizations
In the case of-45 ° of polarized incident, in metallic channel electromagnetic field average normalized after photolytic activity and whole packing space (false
If above packing space height is 1500nm) after average normalized after photolytic activity.Can be seen that at plasma resonance wavelength
Photolytic activity in place's groove reaches maximum, and this is owing to the enhancement effect of surface plasma resonance causes.It is also clear that see
It is symmetrical for going out the photolytic activity produced under symmetry angle polarized incident, namely it is permissible to show to change polarization angle by reverse symmetry
Realize the light chirality of electromagnetic field in switching slot.Notice that the photolytic activity value that accompanying drawing 4 is shown is the result after normalization, that is to say and remove
With the result after the photolytic activity value that left circularly polarised wave equicohesive with incident illumination comprises.
Accompanying drawing 5 assumes that in the structure grooves shown in accompanying drawing 2 and on groove, numerical value, after inserting different chirality sample, is passed through in space
Reflectance spectrum difference signal Δ R under+45 ° and-45 ° polarizations that simulation experiment obtains.According to being analyzed above, this difference signal namely
It it is the circular dichroism of tested chirality sample.In figure, result assumes that the chirality sample being filled is enantiomer, their dielectric ginseng
Number ε=1+0.01i, the Pasteur parameter that their chirality is relevant is κ=+ 0.001i and κ=-0.001i respectively.The symbol generation of κ value
The different chiralitys of table chirality sample.Result is obtained it will be seen that opposite-handed in enantiomer by the present invention program simulation in figure
The circular dichroism spectra of sample is symmetrical about zero line, the overall space average light activity shown in the two circular dichroism and accompanying drawing 4
Trend is consistent, and this and actual theory are consistent, namely demonstrate the feasibility of the present invention program.
Embodiment two
Owing to body structure surface plasma resonance wavelength depends primarily on the size of structure, and generally under plasma resonance wavelength
The photolytic activity produced is maximum, namely it is best to obtain circular dichroism reinforced effects under this wavelength.Therefore, it can by adjusting one-dimensional
Cycle metallic channel physical dimension changes resonant wavelength position so that it and greatest circle two colour response of tested specific molecular own
Wavelength overlaps, and improves detectivity the most to the full extent.The present embodiment is shown in the present invention by changing metallic channel sensing
Device device architecture can change the wavelength of body structure surface plasma resonance, namely photoactive maximum position occurs.
Accompanying drawing 6 is the structure of the One Dimension Periodic metallic channel in embodiment two.The groove cycle is 200nm, groove width 100nm, groove depth
200nm, trench bottom thickness 200nm, the material of groove is gold, and matrix is silicon dioxide；
+ 45 ° and-45 ° when accompanying drawing 7 is metallic channel structure filling achirality sample air in embodiment two (assuming that refractive index is 1)
Reflectance spectrum under linear polarization incidence.Can be seen that compared to the reflectance spectrum result (accompanying drawing 3) under the structure of accompanying drawing 2, embodiment
Reflectance spectrum after physical dimension changes in two also there occurs change.
Accompanying drawing 8 be embodiment two correspondence structure average light activity, incident condition is the same with accompanying drawing 4.Can be seen that
After structural change, corresponding photolytic activity is distributed also with change.
Accompanying drawing 9 is the sensing effect at different chirality samples of the structure in embodiment two, other design conditions and accompanying drawing 5 one
Sample.It can be seen that after One Dimension Periodic groove metallic channel size changes, for the maximum value position of circular dichroism there occurs and change
Become.The Δ R of the different chiral molecules obtained in the present invention program is symmetrical, and this is consistent with theory analysis, the most again demonstrate,proves
Bright native system may be used for the measurement of circular dichroism.Embodiment shows the micro-nano structure size that can be sensed by design
Optimize the position of maximum circular dichroism further, thus realize the detection of the most high-sensitive chirality sample.
Being above presently preferred embodiments of the present invention, all changes made according to technical solution of the present invention, produced function is made
With during without departing from the scope of technical solution of the present invention, belong to protection scope of the present invention.
Claims (9)
1. a circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel measures system, it is characterised in that: include
Light source, the adjustable polarizer, One Dimension Periodic metallic channel structure, sample flow pond and spectrogrph；Described light source can produce the most not
Co-wavelength light；The described adjustable polarizer for being modulated into the line polarized light of the apposing symmetric angle of polarization by incident non-polarized light；Institute
State in One Dimension Periodic metallic channel structure makes incident illumination be radiated at its groove and surface and produce the chirality light field that local strengthens；Described sample
Product flow cell is used for loading liquid tested chirality sample, and is placed in sample flow pond in described One Dimension Periodic metallic channel structure
Time so that liquid tested chirality sample at the uniform velocity flow through One Dimension Periodic metallic channel body structure surface, it is simple to during measurement ensure signal steady
Fixed；Described spectrogrph is for detecting the spectral signal after One Dimension Periodic metallic channel structure reflects；
The light of light source outgoing, after the line polarized light that the adjustable polarizer is modulated into the apposing symmetric angle of polarization, is incident to described one respectively
In dimension cycle metallic channel structure, and in the chirality light field of the different chirality of One Dimension Periodic metallic channel structure generation, due to one-dimensional
Tested chirality sample in cycle metallic channel structure has different absorptions to the light of different chiral so that the apposing symmetric angle of polarization
The reflection produced after line polarized light incidence is different, by producing after the line polarized light incidence of the spectrometer collection apposing symmetric angle of polarization
Reflectance spectrum difference signal can characterize the circular dichroism signal of sample.
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel the most according to claim 1 measures system,
It is characterized in that: described light source is the wide range continuous light source that can produce continuous different wavelengths of light.
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel the most according to claim 1 measures system,
It is characterized in that: the line polarized light of the described apposing symmetric angle of polarization i.e. relative to One Dimension Periodic metallic channel knot constitute+45 ° with-
45 ° ,-30 ° with the line polarized light of+30 ° or-60 ° with+60 °.
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel the most according to claim 1 measures system,
It is characterized in that: described One Dimension Periodic metallic channel structure is the metallic channel of support surface plasma resonance effect.
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel the most according to claim 1 measures system,
Your it is characterized in that: the gold that material is support surface plasma resonance effect that described One Dimension Periodic metallic channel structure uses
Belong to, including gold, silver, aluminum.
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel the most according to claim 1 measures system,
It is characterized in that: the size of described One Dimension Periodic metallic channel structure is sub-wavelength magnitude, i.e. less than light source outgoing wavelength.
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel the most according to claim 1 measures system,
It is characterized in that: described One Dimension Periodic metallic channel structure is sized to be adjusted according to light source outgoing wave length.
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel the most according to claim 1 measures system,
It is characterized in that: described One Dimension Periodic metallic channel structure is for being completely independent structure or being machined on substrate.
Circular dichroism based on line polarized light incidence One Dimension Periodic metallic channel the most according to claim 1 measures system,
It is characterized in that: described sample flow pond is that closing is capable of circulation, and it uses peristaltic pump fluid circulation system to make One Dimension Periodic
The surface of metallic channel structure at the uniform velocity flow through described tested chirality sample.
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