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CN106107206B - Preparation method of fermented chicken feed - Google Patents

Preparation method of fermented chicken feed Download PDF

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CN106107206B
CN106107206B CN201610651240.7A CN201610651240A CN106107206B CN 106107206 B CN106107206 B CN 106107206B CN 201610651240 A CN201610651240 A CN 201610651240A CN 106107206 B CN106107206 B CN 106107206B
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岳春
韩德绸
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Nanyang Institute of Technology
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Abstract

The invention discloses a preparation method of a fermented chicken feed, which comprises the following steps: 1) uniformly mixing strains, wherein the strains comprise aspergillus niger, geotrichum candidum and trichoderma; 2) uniformly mixing the mixed strains with main materials, adding nutrient salt and stirring to obtain a mixed feed, wherein the main materials comprise soybean meal and bran, and the nutrient salt comprises urea, ammonium sulfate, calcium superphosphate, bentonite, salt and water; 3) putting the mixed feed into a fermentation chamber for fermentation, and adding the fermented mixed feed into coarse grains in proportion to prepare fermented feed; 4) and breaking the wall of the rhodotorula benthica to obtain a rhodotorula benthica culture solution, adding the rhodotorula benthica culture solution into the fermented feed, uniformly mixing, and drying to obtain the final chicken feed. The prepared chicken feed contains coarse food grain protein, mycoprotein, protein in rhodotorula benthica, carotenoid and the like, and the promotion and the cooperation among nutrient elements jointly provide a powerful guarantee for the growth of the firewood chicken and enrich the nutrient varieties obtained by the firewood chicken.

Description

一种发酵型鸡饲料的制备方法A kind of preparation method of fermented chicken feed

技术领域technical field

本发明涉及动物饲料技术领域,具体涉及一种发酵型鸡饲料的制备方法。The invention relates to the technical field of animal feed, in particular to a preparation method of a fermented chicken feed.

背景技术Background technique

现代化大规模畜牧业饲养方式,提高了生产速度的同时,也带来了许多问题,畜产品品质降低和畜禽容易发生各种疾病,以及饲料中大量的添加抗生素类药物和添加剂对人类健康造成的危害,这些问题影响着我国畜牧业的健康发展。优质饲料是生产优质畜产品的保证,目前我国迫切需要能提高畜产品的质量和品质,对动物健康生长有益,对消费者的健康有益无害的饲料。The modern large-scale animal husbandry method has improved the production speed, but also brought many problems. The quality of livestock products is reduced, livestock and poultry are prone to various diseases, and a large number of antibiotics and additives in the feed are added to human health. These problems affect the healthy development of my country's animal husbandry. High-quality feed is the guarantee for the production of high-quality livestock products. At present, my country urgently needs feeds that can improve the quality and quality of livestock products, are beneficial to the healthy growth of animals, and are beneficial and harmless to the health of consumers.

特别对于家禽产业,发展更是迅速,各种禽饲料也随之迅速发展,现阶段市场的各种饲料品种繁多,作用各异。但是对于禽饲料来说,两点最为重要,第一点是保证足够的蛋白质含量,为家禽的生长提供充足的条件,降低养殖周期提高收益;第二点是提高家禽的抵抗力,防止虫病危害,提高家禽的存活率。这两点对于较昂贵的家禽来说更是重要,较短的养殖周期使得资金可以快速回笼,利于扩大生产及抵抗突发事件,较高的存活率更使得养殖昂贵家禽时损失较少,间接提高收益。Especially for the poultry industry, the development is even more rapid, and various poultry feeds have also developed rapidly. At this stage, there are many kinds of various feeds in the market, with different functions. But for poultry feed, two points are the most important. The first point is to ensure sufficient protein content to provide sufficient conditions for the growth of poultry, reduce the breeding cycle and increase the income; the second point is to improve the resistance of poultry and prevent insect diseases. harm and improve the survival rate of poultry. These two points are more important for the more expensive poultry. The shorter breeding cycle allows the funds to be quickly returned to the cage, which is conducive to expanding production and resisting emergencies. Increase earnings.

柴鸡具有耐粗饲、适应性、觅食性、遗传性能稳定、就巢性强和抗病力强等特性,目前已在家禽养殖业大量存在,但是柴鸡较一般家禽来说是价值含量较高的种类,如何保证数量较多的蛋白质供给以及如何保证柴鸡的健康状况成为目前养殖需要面对的两个问题。Chai chicken has the characteristics of resistance to rough feeding, adaptability, foraging, stable genetic performance, strong nesting ability and strong disease resistance. At present, it has existed in a large number of poultry breeding industry, but Chai chicken has higher value content than general poultry. How to ensure a large amount of protein supply and how to ensure the health of Chai chicken have become two problems that farming needs to face at present.

申请号为201510299407.3的发明公开了一种发酵型鸡饲料,其特征在于,包括将玉米粉和豆粕粉加入配方中,玉米的重量百分比为45%~50%,豆粕粉10%~15%,所述饲料加入蒸馏水呈溶液状,所述饲料的溶液中加入嗜酸乳酸菌扩培液与枯草芽胞杆菌扩培液,再采用半固体培养基对产朊假丝酵母进行扩大培养成扩培基,将上述饲料溶液与嗜酸乳酸菌扩培液、枯草芽胞杆菌扩培液与产朊假丝酵母扩培基混合密封发酵80小时,发酵后加入草粉。该发明所含的草粉成分可以代替抗生素,维护鸡只的健康,但是蛋白质的供给仍未解决。The invention with application number 201510299407.3 discloses a fermented chicken feed, which is characterized in that it includes adding corn meal and soybean meal powder into the formula, and the weight percentage of corn is 45% to 50% and soybean meal powder is 10% to 15%. The feed is added with distilled water to form a solution, and the solution of the feed is added with Lactobacillus acidophilus expansion solution and Bacillus subtilis expansion solution, and then the semi-solid medium is used to expand and cultivate Candida utilis into an expansion medium, and the The above-mentioned feed solution, Lactobacillus acidophilus expansion solution, Bacillus subtilis expansion solution and Candida utilis expansion medium are mixed and fermented for 80 hours, sealed and fermented for 80 hours, and grass powder is added after fermentation. The grass powder component contained in the invention can replace antibiotics and maintain the health of chickens, but the supply of protein remains unsolved.

申请号为201310651866.4的发明利用发酵玉米秸秆生产高效肉鸡饲料的方法,只需采用玉米秸秆,通过粉碎、氨水处理、蒸汽、发酵等处理,提高玉米秸秆的利用率,降低成本,提升肉鸡饲养率及改善屠宰品质。该方法利用发酵玉米秸秆代替豆粕,降低了成本,但是对于饲料中蛋白质的提升有限,且在一定程度上蛋白质的供给不如纯豆粕多,效果并不理想。The invention with the application number of 201310651866.4 uses fermented corn straw to produce high-efficiency broiler feed. It only needs to use corn straw, and through processing such as pulverization, ammonia treatment, steam, fermentation, etc. Improve slaughter quality. The method uses fermented corn stalks to replace soybean meal, which reduces the cost, but the improvement of protein in the feed is limited, and the protein supply is not as much as pure soybean meal to a certain extent, and the effect is not ideal.

发明内容SUMMARY OF THE INVENTION

有鉴于此,本发明的目的是针对现有技术的不足,提供一种发酵型鸡饲料的制备方法,制得的鸡饲料含有粗粮蛋白、菌体蛋白以及海洋红酵母内部的蛋白质、类胡萝卜素等,将粗粮、白地霉、海洋红酵母结合在一起,营养元素之间的促进与协同,共同为柴鸡的生长提供有力的保障,丰富了柴鸡获得的营养种类。In view of this, the purpose of the present invention is to aim at the deficiencies of the prior art, and to provide a method for preparing a fermented chicken feed. etc., the combination of coarse grains, Geotrichum candidum, and marine red yeast together, the promotion and synergy between nutrient elements provide a strong guarantee for the growth of Chai chicken and enrich the types of nutrients obtained by Chai chicken.

为解决上述技术问题,本发明所采取的技术方案是:In order to solve the above-mentioned technical problems, the technical scheme adopted by the present invention is:

一种发酵型鸡饲料的制备方法,包括如下步骤:A preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉;1) after the bacterial classification is evenly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, salt and water;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料;3) covering the mixed feed with a plastic film, putting it into a fermentation chamber for fermentation, and adding the fermented mixed feed to the coarse grains in proportion to obtain a fermented feed;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

进一步的,所述步骤1)中菌种的各组分重量份数为:黑曲霉1~8份,白地霉4~24份,木霉0.5~2份。Further, the parts by weight of the bacterial species in the step 1) are: 1-8 parts of Aspergillus niger, 4-24 parts of Geotrichum candidum, and 0.5-2 parts of Trichoderma.

进一步的,所述步骤2)中,主料的各组分重量份数为:豆粕50份,麸皮50份;营养盐的各组分重量份数为:尿素1~3份,硫酸铵2~6份,过磷酸钙1~3份,膨润土5~15份,盐0.2~1份,水150~220份。Further, in the step 2), the parts by weight of each component of the main material are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 1 to 3 parts of urea, 2 parts of ammonium sulfate ~6 parts, superphosphate 1-3 parts, bentonite 5-15 parts, salt 0.2-1 part, water 150-220 parts.

进一步的,所述步骤2)中,混合饲料的各组分重量份数为:主料100~300份,菌种10~35份,营养盐110~335份。Further, in the step 2), the parts by weight of each component of the mixed feed are: 100-300 parts of main ingredients, 10-35 parts of bacterial species, and 110-335 parts of nutrient salts.

进一步的,所述步骤3)中,混合饲料的发酵过程分为两段,第一段发酵室温度为30~38℃,发酵时间为6~10h,第二段发酵室温度为22~30℃,发酵时间为18~42h。Further, in the step 3), the fermentation process of the mixed feed is divided into two stages, the temperature of the first stage of the fermentation chamber is 30-38°C, the fermentation time is 6-10h, and the temperature of the second-stage fermentation chamber is 22-30°C , the fermentation time is 18 ~ 42h.

进一步的,所述步骤3)中,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕和麸皮中的一种或多种。Further, in the step 3), the parts by weight of each component of the fermented feed are: 1 part of the fermented mixed feed and 4 parts of coarse grains, and the coarse grains include one or more of soybean meal and bran.

进一步的,所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:Further, in the described step 4), the preparation method of the marine red yeast culture liquid comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在26~34℃下活化40~56h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 26-34°C for 40-56h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在24~32℃、200~400r/min下离心培养20~28h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 24-32°C and 200-400r/min for 20-28h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在26~34℃、200~400r/min下离心培养60~84h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 26-34°C and 200-400r/min for 60-84h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在30~65℃、200~400r/min转速下旋转振荡3~7h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量不大于0.2g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and oscillate at 30 to 65 ° C and 200 to 400 r/min for 3 to 7 hours to obtain a marine Rhododendron culture solution. Each 100 mL of fermentation The weight of β-mannanase added to the fermentation medium after cultivation is not more than 0.2 g.

进一步的,所述斜面培养基每升中包含葡萄糖15~35g,蛋白陈5~11g,酵母粉3~7g,琼脂10~30g。Further, each liter of the slant medium contains 15-35 g of glucose, 5-11 g of protein, 3-7 g of yeast powder, and 10-30 g of agar.

进一步的,所述液体种子培养基每升中包含葡萄糖15~35g,蛋白陈5~11g,酵母粉3~7g,磷酸二氢钾4~8g,氯化钠5~15g,硫酸镁2~4g,PH值为4~7,所述发酵培养基与所述液体种子培养基成分相同。Further, each liter of the liquid seed culture medium contains 15-35 g of glucose, 5-11 g of protein, 3-7 g of yeast powder, 4-8 g of potassium dihydrogen phosphate, 5-15 g of sodium chloride, and 2-4 g of magnesium sulfate. , the pH value is 4-7, and the fermentation medium and the liquid seed medium have the same composition.

进一步的,所述每克发酵饲料加入1mL海洋红酵母培养液。Further, 1 mL of marine red yeast culture solution is added to each gram of the fermented feed.

本发明提供了一种发酵型鸡饲料的制备方法,通过在主料中加入白地霉,可以将主料中的蛋白大量转化为菌丝蛋白,产生利于柴鸡吸收的菌体蛋白,提高饲料中蛋白质的利用率,黑曲霉和木霉优先培养,可以生成大量的有机酸等营养物质,刺激白地霉生产更多的菌体蛋白,对发酵饲料的蛋白质转化具有很强的促进作用。营养盐为白地霉、黑曲霉和木霉的培养提供更好的环境,促进其菌种的培养,且符合的多种维生素和矿物质也丰富了鸡饲料的营养成分,利于柴鸡的健康生长。The invention provides a preparation method of a fermented chicken feed. By adding Geotrichum candidum to the main material, a large amount of protein in the main material can be converted into mycelial protein, so as to produce bacterial protein that is beneficial to the absorption of Chai chicken, and improve the protein in the feed. The utilization rate of Aspergillus niger and Trichoderma are preferentially cultivated, which can generate a large amount of nutrients such as organic acids, stimulate the production of more bacterial protein by Geotrichum candidum, and have a strong promotion effect on the protein transformation of fermented feed. Nutrient salts provide a better environment for the cultivation of Geotrichum candidum, Aspergillus niger and Trichoderma, and promote the cultivation of their strains, and the multivitamins and minerals that meet the requirements also enrich the nutritional components of chicken feed, which is conducive to the healthy growth of Chai chickens.

发酵之后的混合饲料与粗粮混合,这样可以保证混合饲料的粗蛋白向菌体蛋白的转化率,也就是将白地霉、黑曲霉和木霉的作用彻底发挥出来,然后再加入未发酵的粗粮,在控制成本的前提下,既能保证饲料内的菌体蛋白含量,也能保证植物蛋白的含量,使得柴鸡获得的营养更加均衡。粗粮的选择有很多,推荐使用的是豆粕、麸皮,再具体的使用中根据饲料蛋白质的吸收进行判断,也可以对粗粮进行轮换使用,丰富饲料的营养。The fermented mixed feed is mixed with coarse grains, so as to ensure the conversion rate of crude protein of mixed feed to bacterial protein, that is, the functions of Geotrichum candidum, Aspergillus niger and Trichoderma can be fully exerted, and then unfermented coarse grains are added. Under the premise of controlling costs, it can not only ensure the content of bacterial protein in the feed, but also ensure the content of vegetable protein, so that the nutrition obtained by Chai chicken is more balanced. There are many choices of coarse grains. The recommended ones are soybean meal and bran. The specific use should be judged according to the absorption of feed protein. Coarse grains can also be used in rotation to enrich the nutrition of the feed.

海洋红酵母是自然海域中存在的一种酵母菌。其细胞中富含蛋白质、肝糖、不饱和脂肪酸、动物幼体生长激素及以虾青素为主的类胡萝卜素等多种营养物质。该酵母对于动物幼体的成长具有很好的作用,增强抵抗力、加快生长速度等,但是尚未大量应用在鸡饲料内,本发明中通过添加海洋红酵母,再次增加了饲料内的蛋白质含量,且使得饲料内含有类胡萝卜素,对于柴鸡的成长极为有利,也增加了柴鸡的感官色泽与营养价值,使得柴鸡鸡蛋营养价值更高、味道更好,而柴鸡本身羽毛鲜亮,肉质鲜美,提升产品的竞争力。本发明在添加海洋红酵母之前对其进行破壁处理,增加细胞壁的通透性,利于细胞内的虾青素等营养物质渗透至细胞外被柴鸡吸收,大大增加营养物质被吸收的概率,提高海洋红酵母的利用率。Marine red yeast is a yeast that exists in natural sea areas. Its cells are rich in protein, glycogen, unsaturated fatty acids, larval growth hormone and carotenoids mainly astaxanthin and other nutrients. The yeast has a very good effect on the growth of animal larvae, enhances resistance, accelerates growth rate, etc., but has not been widely used in chicken feed. In the present invention, by adding marine red yeast, the protein content in the feed is increased again, and It makes the feed contain carotenoids, which is extremely beneficial to the growth of Chai chicken, and also increases the sensory color and nutritional value of Chai chicken, making Chai chicken eggs higher in nutritional value and taste better. Competitiveness. The present invention performs wall-breaking treatment on the marine red yeast before adding it to increase the permeability of the cell wall, which facilitates the penetration of nutrients such as astaxanthin in the cell to the outside of the cell and is absorbed by Chai chicken, greatly increases the probability of the nutrients being absorbed, and improves the Utilization of marine red yeast.

海洋红酵母的破壁方法采用了酶法破壁,首先对海洋红酵母进行活化、培养、发酵,选取更优质的酵母进行培养发酵,得到的海洋红酵母质量更高。再通过加入β-甘露聚糖酶可以改变酵母细胞壁通透性,实现对海洋红酵母的破壁,该破壁方法改善了化学破壁法对虾青素的破坏,也改善了其它酶解方法造成的饲料口味变差的缺陷,大大提高了海洋红酵母的破壁率,并且β-甘露聚糖酶还可以促进能量、蛋白、纤维素的消化和吸收,增强柴鸡对饲料营养的吸收效果。The method of breaking the wall of marine red yeast adopts the enzymatic method to break the wall. First, the marine red yeast is activated, cultured, and fermented, and then higher-quality yeast is selected for cultivation and fermentation, and the obtained marine red yeast is of higher quality. Then by adding β-mannanase, the permeability of yeast cell wall can be changed, and the wall of marine red yeast can be broken. The defect of poor feed taste greatly improves the wall-breaking rate of marine red yeast, and β-mannanase can also promote the digestion and absorption of energy, protein and cellulose, and enhance the absorption effect of Chai chicken on feed nutrients.

最终的鸡饲料为发酵饲料与海洋红酵母培养液的混合,其含有粗粮蛋白、菌体蛋白以及海洋红酵母内部的蛋白质、类胡萝卜素等,将粗粮、白地霉、海洋红酵母结合在一起,营养元素之间的促进与协同,共同为柴鸡的生长提供有力的保障,丰富了柴鸡获得的营养种类,也提高了其对鸡饲料内各成分尤其是蛋白质的吸收率,大大加快了柴鸡的生长速度,改善了主副产品的口感、色泽以及营养价值。The final chicken feed is a mixture of fermented feed and marine red yeast culture liquid, which contains coarse grain protein, bacterial protein, protein and carotenoids inside the marine red yeast, etc., combined with coarse grains, Geotrichum candidum and marine red yeast, The promotion and synergy between nutrient elements together provide a strong guarantee for the growth of Chai chicken, enrich the types of nutrients obtained by Chai chicken, and also improve its absorption rate of various components in chicken feed, especially protein, which greatly accelerates the growth of Chai chicken. Speed, improve the taste, color and nutritional value of the main and by-products.

本发明的有益效果如下:The beneficial effects of the present invention are as follows:

1、黑曲霉和木霉的培养为白地霉提供有利的生长环境,使得白地霉对植物蛋白的转换更加彻底,生产更多的菌体蛋白,较单一使用白地霉效果更好;1. The cultivation of Aspergillus niger and Trichoderma provides a favorable growth environment for Geotrichum candidum, which makes the transformation of plant protein by Geotrichum candidum more thorough, and produces more bacterial protein, which is better than the single use of Geotrichum candidum;

2、营养盐为白地霉、黑曲霉和木霉的培养提供更好的环境,促进其菌种的培养,且符合的多种维生素和矿物质也丰富了鸡饲料的营养成分,利于柴鸡的健康生长;2. Nutrient salts provide a better environment for the cultivation of Geotrichum candidum, Aspergillus niger and Trichoderma, promote the cultivation of their strains, and the multivitamins and minerals that meet the requirements also enrich the nutritional components of chicken feed, which is beneficial to the health of Chai chickens grow;

3、发酵之后的混合饲料与粗粮混合,在控制成本的前提下,既能保证饲料内的菌体蛋白含量,也能保证植物蛋白的含量,使得柴鸡获得的营养更加均衡;3. The mixed feed after fermentation is mixed with coarse grains. Under the premise of controlling costs, it can not only ensure the content of bacterial protein in the feed, but also ensure the content of vegetable protein, so that the nutrition obtained by Chai chicken is more balanced;

4、通过添加海洋红酵母,再次增加了饲料内的蛋白质含量,且使得饲料内含有类胡萝卜素,对于柴鸡的成长极为有利,也增加了柴鸡的感官色泽与营养价值,使得柴鸡鸡蛋营养价值更高、味道更好,而柴鸡本身羽毛鲜亮,肉质鲜美,提升产品的竞争力;4. By adding marine red yeast, the protein content in the feed is increased again, and the feed contains carotenoids, which is extremely beneficial to the growth of Chai chicken, and also increases the sensory color and nutritional value of Chai chicken, making the nutritional value of Chai chicken eggs. High, tastes better, and Chai chicken itself has bright feathers and delicious meat, which enhances the competitiveness of the product;

5、采用β-甘露聚糖酶进行酶解对海洋红酵母进行破壁,能很好地避免化学破壁法对虾青素等多种营养物质被破坏,也改善了其它酶解方法造成的饲料口味变差的缺陷,并且β-甘露聚糖酶还可以促进能量、蛋白、纤维素的消化和吸收,增强柴鸡对饲料营养的吸收效果。5. The use of β-mannanase for enzymatic hydrolysis to break the wall of marine red yeast can well avoid the destruction of various nutrients such as astaxanthin by chemical wall breaking method, and also improve the feed caused by other enzymatic hydrolysis methods. Defects of poor taste, and β-mannanase can also promote the digestion and absorption of energy, protein and cellulose, and enhance the absorption effect of Chai chicken on feed nutrients.

具体实施方式Detailed ways

为了更好地理解本发明,下面结合实施例进一步清楚阐述本发明的内容,但本发明的保护内容不仅仅局限于下面的实施例,具体实施方式如下:In order to better understand the present invention, the content of the present invention is further clearly described below in conjunction with the examples, but the protection content of the present invention is not limited to the following examples, and the specific embodiments are as follows:

实施例一Example 1

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕,后续实施例均采用豆粕保证试验的准确性;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. Including soybean meal, the subsequent embodiments all use soybean meal to ensure the accuracy of the test;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation, and then rotate and vibrate for 5 hours at 50 ° C and 300r/min to obtain a marine Rhododendron culture solution. Each 100mL of fermentation medium after fermentation The weight of β-mannanase added inside is 0.05g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例二Embodiment 2

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括白地霉;1) after the bacterial classification is evenly mixed, the bacterial classification includes Geotrichum candidum;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例三Embodiment 3

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料150份,菌种22份,营养盐160份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 150 parts of main ingredients, 22 parts of bacterial species, and 160 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例四Embodiment 4

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料260份,菌种22份,营养盐280份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 260 parts of main ingredients, 22 parts of bacterial species, and 280 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例五Embodiment 5

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室在25℃下发酵38h,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) Cover the mixed feed with plastic film, put it into the fermentation room for fermentation at 25°C for 38 hours, add the fermented mixed feed to the coarse grains in proportion to obtain fermented feed, and the parts by weight of each component of the fermented feed are: 1 part of mixed feed and 4 parts of coarse grains, the coarse grains include soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例六Embodiment 6

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为31℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 31°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例七Embodiment 7

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为29℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 29°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例八Embodiment 8

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料2份,粗粮3份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例九Embodiment 9

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕,将发酵饲料烘干制得最终鸡饲料。3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. Including soybean meal, the fermented feed is dried to produce the final chicken feed.

实施例十Embodiment ten

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)将海洋红酵母培养液加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) adding the marine red yeast culture liquid into the fermented feed, mixing evenly and drying to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例十一Embodiment 11

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在40℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 40°C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为5,所述发酵培养基与所述液体种子培养基成分相同。The liquid seed medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and a pH value of 5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例十二Embodiment 12

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为4.5,所述发酵培养基与所述液体种子培养基成分相同。Each liter of the liquid seed culture medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and the pH value is 4.5. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例十三Embodiment thirteen

本实施例提供了一种发酵型鸡饲料的制备方法,包括如下步骤:The present embodiment provides a preparation method of fermented chicken feed, comprising the following steps:

1)将菌种混合均匀后,所述菌种包括黑曲霉、白地霉和木霉,所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;1) after the bacterial classification is uniformly mixed, the bacterial classification includes Aspergillus niger, Geotrichum candidum and Trichoderma, and the parts by weight of each component of the bacterial classification are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma ;

2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料包括豆粕和麸皮,所述营养盐包括尿素、硫酸铵、过磷酸钙、膨润土、盐和水,所述主料的各组分重量份数为:豆粕50份,麸皮50份;所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份,所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient includes soybean meal and bran, and the nutrient salt includes urea, ammonium sulfate, superphosphate, bentonite, Salt and water, the parts by weight of each component of the main ingredient are: 50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, superphosphoric acid 2 parts of calcium, 10 parts of bentonite, 0.6 part of salt, 180 parts of water, and the parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts;

3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料,所述混合饲料的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕;3) The mixed feed is covered with a plastic film, put into a fermentation chamber for fermentation, and the fermented mixed feed is added to the coarse grains in proportion to obtain a fermented feed. The fermentation process of the mixed feed is divided into two sections, and the temperature of the first section of the fermentation room is divided into two sections. The fermentation time is 34°C, the fermentation time is 8h, the temperature of the second fermentation chamber is 25°C, and the fermentation time is 30h. including soybean meal;

4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料。4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, mixing it evenly, and drying it to obtain the final chicken feed.

所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:The preparation method of the marine red yeast culture liquid in the described step 4) comprises the following steps:

a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在30℃下活化48h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 30°C for 48h;

b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在28℃、300r/min下离心培养24h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 28°C and 300r/min for 24h;

c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在30℃、300r/min下离心培养72h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 30°C and 300r/min for 72h;

d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在50℃、300r/min转速下旋转振荡5h旋转振荡5h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量位0.05g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and shake for 5 hours at 50 ° C and 300r/min rotation speed to obtain a marine Rhododendron culture solution. The weight of β-mannanase added to the fermentation medium was 0.05 g.

所述斜面培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,琼脂20g。Each liter of the slant medium contains 25 g of glucose, 8 g of protein, 5 g of yeast powder, and 20 g of agar.

所述液体种子培养基每升中包含葡萄糖25g,蛋白陈8g,酵母粉5g,磷酸二氢钾5.6g,氯化钠10g,硫酸镁3g,PH值为6,所述发酵培养基与所述液体种子培养基成分相同。Each liter of the liquid seed culture medium contains 25g of glucose, 8g of protein, 5g of yeast powder, 5.6g of potassium dihydrogen phosphate, 10g of sodium chloride, 3g of magnesium sulfate, and the pH value is 6. The liquid seed medium composition is the same.

每克发酵饲料加入1mL海洋红酵母培养液。Add 1 mL of marine red yeast culture broth per gram of fermented feed.

实施例一至实施例八保证海洋红酵母的破壁条件避免,对前三个步骤进行改变,实施例九至实施例十三是对前三个步骤保持不变,对海洋红酵母是否添加、是否破壁以及破壁方法进行试验。将1300只柴鸡平均分为13组使用上述十三种饲料,对饲料以及柴鸡粪便进行检测,得到表格如下:Examples 1 to 8 ensure that the wall-breaking conditions of marine red yeast are avoided, and the first three steps are changed. Examples nine to thirteen are to keep the first three steps unchanged, whether to add marine red yeast, whether to add Wall-breaking and wall-breaking methods were tested. Divide 1300 Chai chickens into 13 groups on average and use the above thirteen kinds of feeds to test the feed and Chai chicken feces. The table is as follows:

表二:Table II:

Figure BDA0001074740880000291
Figure BDA0001074740880000291

Figure BDA0001074740880000301
Figure BDA0001074740880000301

粗蛋白含量是对最终鸡饲料的粗蛋白含量进行检测;类胡萝卜素含量是对鸡饲料的类胡萝卜素进行检测;蛋白质吸收率检测是对柴鸡粪便的粗蛋白进行检测,通过与鸡饲料的粗蛋白进行计算,公式为(鸡饲料粗蛋白含量-粪便蛋白质含量)/鸡饲料粗蛋白含量;病亡率为养殖一个月内的柴鸡病亡率;海洋红酵母破壁率是对海洋红酵母培养液内的海洋红酵母破壁情况进行检测,破壁率越高越好。The crude protein content is to detect the crude protein content of the final chicken feed; the carotenoid content is to detect the carotenoid of the chicken feed; the protein absorption rate is to detect the crude protein of the feces of the Chai chicken, and the crude protein content of the chicken feed is detected by comparing with the crude protein of the chicken feed. The protein is calculated by the formula (crude protein content of chicken feed - protein content of feces)/crude protein content of chicken feed; the mortality rate is the mortality rate of Chai chicken within one month of breeding; The wall-breaking situation of marine red yeast in the liquid is detected, and the higher the wall-breaking rate, the better.

由上表可看出实施例一的效果最好,其粗蛋白含量最高,类胡萝卜素因为海洋红酵母培养方法一致,所以除了实施例九其它实施例都一样,但是考虑破壁率实施例十到实施例十三在类胡萝卜素吸收上表现一般。在蛋白质吸收率上实施例一吸收率最高,病亡率为0,说明其内部的类胡萝卜素吸收较好,菌体蛋白、植物蛋白和海洋红酵母渗透出的蛋白质吸收最好。It can be seen from the above table that the effect of Example 1 is the best, its crude protein content is the highest, and the carotenoid is the same because of the same culture method of marine red yeast, so except for Example 9, other examples are the same, but consider the wall-breaking rate Example 10 In Example 13, the carotenoid absorption was average. In terms of protein absorption rate, the absorption rate of Example 1 is the highest, and the mortality rate is 0, indicating that the internal carotenoid absorption is better, and the protein infiltration of bacterial protein, vegetable protein and marine red yeast is the best.

Claims (6)

1.一种发酵型柴鸡饲料的制备方法,其特征在于:包括如下步骤:1. a preparation method of fermented Chai chicken feed, is characterized in that: comprise the steps: 1)将菌种混合均匀后,所述菌种由黑曲霉、白地霉和木霉组成;1) after the bacterial classification is mixed, the bacterial classification is made up of Aspergillus niger, Geotrichum candidum and Trichoderma; 2)将混合后的菌种与主料混合均匀,加入营养盐并搅拌,得到混合饲料,所述主料为豆粕和麸皮,所述营养盐为尿素、硫酸铵、过磷酸钙、膨润土、盐和水;2) mix the mixed bacterial classification with the main ingredient, add nutrient salt and stir to obtain a mixed feed, the main ingredient is soybean meal and bran, and the nutrient salt is urea, ammonium sulfate, superphosphate, bentonite, salt and water; 3)将混合饲料覆盖塑料膜,放入发酵室进行发酵,将发酵后的混合饲料按比例加入粗粮中制得发酵饲料;3) covering the mixed feed with a plastic film, putting it into a fermentation chamber for fermentation, and adding the fermented mixed feed to the coarse grains in proportion to obtain a fermented feed; 4)对海洋红酵母进行破壁制得海洋红酵母培养液,将其加入发酵饲料中,混合均匀后烘干制得最终鸡饲料;4) breaking the wall of the marine red yeast to obtain the marine red yeast culture liquid, adding it to the fermented feed, and drying after mixing to obtain the final chicken feed; 所述步骤1 )中菌种的各组分重量份数为:黑曲霉1~8份,白地霉4~24份,木霉0 .5~2份;The parts by weight of the bacterial species in the step 1) are: 1-8 parts of Aspergillus niger, 4-24 parts of Geotrichum candidum, and 0.5-2 parts of Trichoderma; 豆粕50份,麸皮50份;营养盐的各组分重量份数为:尿素1~3份,硫酸铵2~6份,过磷酸钙1~3份,膨润土5~15份,盐0 .2~1份,水150~220份;50 parts of soybean meal, 50 parts of bran; the parts by weight of each component of the nutrient salt are: 1-3 parts of urea, 2-6 parts of ammonium sulfate, 1-3 parts of superphosphate, 5-15 parts of bentonite, and 0.5 parts of salt. 2 to 1 parts, 150 to 220 parts of water; 所述步骤2 )中,混合饲料的各组分重量份数为:主料100~300份,菌种10~35份,营养盐110~335份;In the step 2), the parts by weight of each component of the mixed feed are: 100-300 parts of main ingredients, 10-35 parts of bacterial species, and 110-335 parts of nutrient salts; 所述步骤3 )中,混合饲料的发酵过程分为两段,第一段发酵室温度为30~38℃,发酵时间为6~10h,第二段发酵室温度为22~30℃,发酵时间为18~42h。In the step 3), the fermentation process of the mixed feed is divided into two stages, the temperature of the first stage of the fermentation chamber is 30-38°C, the fermentation time is 6-10h, the temperature of the second-stage fermentation chamber is 22-30°C, and the fermentation time is 22-30°C. For 18 ~ 42h. 2.如权利要求1所述的发酵型柴鸡饲料的制备方法,其特征在于:步骤1)所述菌种的各组分重量份数为:黑曲霉4份,白地霉16份,木霉1份;2. The preparation method of fermented Chaiji feed as claimed in claim 1, characterized in that: in step 1) the parts by weight of each component of the bacterial species are: 4 parts of Aspergillus niger, 16 parts of Geotrichum candidum, 1 part of Trichoderma share; 步骤2)所述主料的各组分重量份数为:豆粕50份,麸皮50份;Step 2) The parts by weight of the main ingredients are: 50 parts of soybean meal and 50 parts of bran; 所述营养盐的各组分重量份数为:尿素2份,硫酸铵4份,过磷酸钙2份,膨润土10份,盐0.6份,水180份;The parts by weight of each component of the nutrient salt are: 2 parts of urea, 4 parts of ammonium sulfate, 2 parts of superphosphate, 10 parts of bentonite, 0.6 parts of salt, and 180 parts of water; 所述混合饲料的各组分重量份数为:主料200份,菌种22份,营养盐222份;The parts by weight of each component of the mixed feed are: 200 parts of main ingredients, 22 parts of bacterial species, and 222 parts of nutrient salts; 步骤3)所述的发酵过程分为两段,第一段发酵室温度为34℃,发酵时间为8h,第二段发酵室温度为25℃,发酵时间为30h,发酵饲料的各组分重量份数为:发酵后的混合饲料1份,粗粮4份,所述粗粮包括豆粕。The fermentation process described in step 3) is divided into two stages, the temperature of the first stage of the fermentation chamber is 34°C and the fermentation time is 8h, the temperature of the second stage of the fermentation chamber is 25°C and the fermentation time is 30h, and the weight of each component of the fermented feed is The number of servings is: 1 part of the fermented mixed feed and 4 parts of coarse grains, and the coarse grains include soybean meal. 3.如权利要求2所述的发酵型柴鸡饲料的制备方法,其特征在于:所述步骤4)中海洋红酵母培养液的制备方法包括如下步骤:3. the preparation method of fermented Chai chicken feed as claimed in claim 2 is characterized in that: the preparation method of marine red yeast culture liquid in described step 4) comprises the steps: a.菌株活化:将保藏于冰箱中的海洋红酵母菌种接种在斜面培养基上在26~34℃下活化40~56h;a. Strain activation: Inoculate the marine Rhododendron strain stored in the refrigerator on the slant medium and activate it at 26-34°C for 40-56h; b.种子培养:将斜面培养基内充分活化的菌种接种到液体种子培养基,在24~32℃、200~400r/min下离心培养20~28h;b. Seed culture: inoculate the fully activated strains in the slant medium into the liquid seed medium, and centrifuge at 24-32°C and 200-400r/min for 20-28h; c.发酵培养:将培养后的菌种以10%接种量接入到发酵培养基中,在26~34℃、200~400r/min下离心培养60~84h;c. Fermentation culture: insert the cultured strains into the fermentation medium at 10% inoculum, and centrifuge at 26-34°C and 200-400r/min for 60-84h; d.将β-甘露聚糖酶加入发酵培养后的发酵培养基中,然后在30~65℃、200~400r/min转速下旋转振荡3~7h制得海洋红酵母培养液,每100mL的发酵培养后的发酵培养基内加入β-甘露聚糖酶的重量不大于0.2g。d. Add β-mannanase to the fermentation medium after fermentation and culture, and then rotate and oscillate at 30 to 65 ° C and 200 to 400 r/min for 3 to 7 hours to obtain a marine Rhododendron culture solution. Each 100 mL of fermentation The weight of β-mannanase added to the fermentation medium after cultivation is not more than 0.2 g. 4.如权利要求3所述的发酵型鸡饲料的制备方法,其特征在于:所述斜面培养基每升中包含葡萄糖15~35g,蛋白陈5~11g,酵母粉3~7g,琼脂10~30g。4. The preparation method of fermented chicken feed according to claim 3, wherein the slant medium contains 15-35 g of glucose, 5-11 g of protein, 3-7 g of yeast powder, and 10-10 g of agar in each liter of the slant medium. 30g. 5.如权利要求4所述的发酵型鸡饲料的制备方法,其特征在于:所述液体种子培养基每升中包含葡萄糖15~35g,蛋白陈5~11g,酵母粉3~7g,磷酸二氢钾4~8g,氯化钠5~15g,硫酸镁2~4g,PH值为4~7,所述发酵培养基与所述液体种子培养基成分相同。5. The preparation method of fermented chicken feed as claimed in claim 4, wherein the liquid seed medium contains 15-35 g of glucose, 5-11 g of protein, 3-7 g of yeast powder, 2-phosphoric acid per liter of the liquid seed medium. Potassium hydrogen 4~8g, sodium chloride 5~15g, magnesium sulfate 2~4g, pH value is 4~7, described fermentation medium and described liquid seed medium have the same composition. 6.如权利要求5所述的发酵型鸡饲料的制备方法,其特征在于:所述每克发酵饲料加入1mL海洋红酵母培养液。6. The preparation method of fermented chicken feed as claimed in claim 5, characterized in that: 1 mL of marine red yeast culture solution is added to each gram of fermented feed.
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