CN105815324B - Bactericidal composition containing oxazole and 2-cyano-3-amino-3-phenylancryic acetate - Google Patents
Bactericidal composition containing oxazole and 2-cyano-3-amino-3-phenylancryic acetate Download PDFInfo
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Abstract
The invention belongs to pesticide fields, and in particular to a kind of bactericidal composition containing oxazole and 2-cyano-3-amino-3-phenylancryic acetate and its application.The composition is made of active constituent, filler and auxiliary agent, wherein active constituent is made of oxazole and 2-cyano-3-amino-3-phenylancryic acetate, the composition has good control effect to the plurality of plant diseases caused by sac fungus, basidiomycetes and Fungi Imperfecti, can effectively control the generation and harm of the plant diseases such as graw mold of tomato, gray mold of cucumber, leaf muld of tomato, alternaria leaf spot of apple, wheat scab, cotton wilt, banana Panama disease, bakanae disease of rice, watermelon blight.The composition wide sterilization spectrum has apparent synergistic effect, can reduce dosage, reduces drug cost, and germ is delayed to generate and develop the resistance of single medicine.
Description
Technical field
The invention belongs to pesticide fields, and in particular to a kind of bactericidal composition containing oxazole and 2-cyano-3-amino-3-phenylancryic acetate and its
Using.
Background technology
Oxazole (English common name：Pyrisoxazole it is) that the pyridyloxazole quinoline class that Shenyang Chemical Engineering Inst develops is new
Type disinfectant use in agriculture.Oxazole chemical name is：- oxazole of N- methyl -3- (4- chlorine) phenyl -5- methyl -5- pyridine -3- methyl
Quinoline, molecular formula C16H17ClN2O；Its CAS accession number [847749-37-5], structural formula is as follows：
The oxazole mechanism of action is to inhibit the synthesis of plant pathogenic fungi sterol.By in sterol biosynthesis approach
Various enzymes are had an effect, and the biosynthesis of ergosterol is interfered or block, and are destroyed the structure of disease fungus cell membrane and are reached suppression
Bacterium and sterilization purpose.Oxazole structure novel, the mode of action is unique, wide sterilization spectrum, and bactericidal activity is high, has to plant disease
Preventive and therapeutic action is widely used in preventing corps diseases, to a variety of caused by sac fungus, basidiomycetes and Fungi Imperfecti
Plant disease has good control effect, can effectively control graw mold of tomato, gray mold of cucumber, leaf muld of tomato, apple spot
The generation and harm of the plant diseases such as point defoliation, wheat scab.Meanwhile oxazole to soil-borne disease also have it is good
Preventive effect.
2-cyano-3-amino-3-phenylancryic acetate is the new type bactericide that Jiangsu Pesticide Research Institute Co., Ltd is independently formulated.Chemical name：
2- cyano -3- aminoacrylic acid ethyl esters, molecular formula C12H12N2O2.Active compound appearance is white solid powder；Fusing point 123-124
℃；Vapour pressure (25 DEG C)：4.5*10-5Pa；Solubility (20 DEG C)：Be insoluble in water, petroleum ether, toluene, be soluble in chloroform, acetone,
Dimethyl sulfoxide (DMSO), N,N-dimethylformamide.Stability：Stablize in acid, alkaline medium, light is stablized.Its chemistry knot
Structure formula is：
2-cyano-3-amino-3-phenylancryic acetate has Fusariumsp the compound of greater activity, especially to Fusarium graminearum (Fusarium
Graminearum) mycelia growth has strong inhibitory activity.The compound is efficient, micro- poison, wide spectrum, low-residual, to environment friend
It is good, there is protection and therapeutic effect to the plant disease caused by sickle-like bacteria, wheat is red caused by can be applied to prevention sickle-like bacteria
Mildew, cotton wilt, banana Panama disease, bakanae disease of rice, watermelon blight etc..
Invention content
Present invention aims at the bactericidal composition containing oxazole and 2-cyano-3-amino-3-phenylancryic acetate is provided, it is by two kinds of different knots
Structure, different role mechanism sterilization component compounded, with expand prevention spectrum and improve drug effect, the anti-medicine of pathogen can also be delayed
The generation and development of property, extend the service life of medicament.
The present invention is a kind of bactericidal composition made of oxazole and 2-cyano-3-amino-3-phenylancryic acetate compounding, is screened by formula indoor
Measure experiment, mixture the test of pesticide effectiveness, both clear rational formula, i.e. the weight part ratio of oxazole and 2-cyano-3-amino-3-phenylancryic acetate is
1:90-90:1, preferably 1:60-60:1, optimal is 1:20-20:1.Oxazole and the weight of 2-cyano-3-amino-3-phenylancryic acetate active constituent account for
The 1%-80% of composition total weight, preferably 5%-60%.
The present composition can be prepared into a kind of dosage form of suitable agricultural use by known method, and relatively good dosage form has
It is aqueous suspension agent, dispersible oil-suspending agent, aqueous emulsion, microemulsion, wettable powder, soluble powder, suspension seed-coating agent, water-dispersed
Granula, granule, seed treatment microcapsule suspending agent and missible oil etc..
The auxiliary agent used in the composition of the present invention includes emulsifier, dispersant, wetting agent, stabilizer, antifreezing agent, thickening
Agent etc. and other active ingredients that are beneficial to stablize the substance played with drug effect in the formulation, these auxiliary agents are commonly used in pesticidal preparations
Or allow the various composition used, it is not particularly limited, specific ingredient and dosage can be according to the recipe requirements determined by the test.This
A kind of purposes of controlling plant diseases of disclosure of the invention.The composition can be used on the crops such as Cereal, fruit tree and vegetables preventing
Plurality of plant diseases caused by sac fungus, basidiomycetes and Fungi Imperfecti etc., such as graw mold of tomato, gray mold of cucumber, tomato leaf mold
Disease, alternaria leaf spot of apple, wheat scab, watermelon blight etc..
Include that the composition of the present invention is applied to cereal the invention also discloses a kind of method of controlling plant diseases
The crops such as class, fruit tree and vegetables, for preventing the diseases such as gray mold, leaf mold, spot defoliation, head blight, droop.The party
In method, the component of the active constituent including the composition can be both made to preparation application, it also can will be including the composition
It is applied after the component bucket of active constituent is mixed.
The present composition has following remarkable result and advantage：
1. synergistic effect is apparent：Experiments have shown that the bactericidal composition of oxazole and 2-cyano-3-amino-3-phenylancryic acetate has apparent increase to disease
Effect acts on.
2. expanding prevention spectrum：The bactericidal composition of oxazole and 2-cyano-3-amino-3-phenylancryic acetate can prevent a variety of diseases in agricultural production
Evil.
3. reducing use cost：Preventive effect, prevention spectrum expansion are improved because compounding has, reduces access times, reducing makes
Use cost.
4. delaying drug resistance：The ingredient compounding of two kinds of different role mechanism, pathogen are not likely to produce drug resistance, delay cause of disease
Generation and development of the object to single dose resistance.
Specific implementation mode：
The present invention is described by the following specific embodiments, but is not limited to these embodiments.The effect of the present invention is real
It tests using indoor biometrics in such a way that field trial is combined.Unless otherwise instructed, ratio mentioned below is all quality percentage
Than.
Following example of formulations is the further explanation to the present invention, but the present invention is not limited solely in present embodiment
Preparation compositions.Percentage composition is mass percent in all formulations proportioning.Active compound and pesticide employed in embodiment help
Agent is that market purchase obtains, and the model of auxiliary agent is also known in the art to be known.
Embodiment 1：16% oxazole 2-cyano-3-amino-3-phenylancryic acetate microemulsion
Weigh 8g oxazole active compound, 8g 2-cyano-3-amino-3-phenylancryic acetate active compounds, 8g N-Methyl pyrrolidones, 17g isopropanols, 4g agriculture breasts
600#, 6g agriculture breast 1601#, add water to complement to 100g.Active compound, solvent, emulsifier are added together, make to be dissolved into homogeneous oil phase；
Water-soluble component and water are mixed to prepare water phase；Under high velocity agitation, oil phase is mixed with water, it is preferable that physical and chemical performance is made
Microemulsion.
Embodiment 2：20% oxazole 2-cyano-3-amino-3-phenylancryic acetate suspending agent
Weigh 15g oxazole active compound, 5g 2-cyano-3-amino-3-phenylancryic acetates active compound, 8g FS3000 (Phosphate Anionic Surfactants),
1gTXC (sulfonate anionic surfactant), 0.3g xanthans, 1g aluminium-magnesium silicates, 2g ethylene glycol, 0.2g BIT (1,2- benzene
And isothiazoline -3- ketone), the organically-modified silica alkane antifoaming agent of 0.3g, deionized water adds to 100g.Above-mentioned raw materials are added to height
10min is sheared in emulsification pretreatment machine, then after being sanded with sand mill, obtain suspending agent.
Embodiment 3：60% oxazole 2-cyano-3-amino-3-phenylancryic acetate water dispersible granules
Weigh 50g oxazole active compound, 10g 2-cyano-3-amino-3-phenylancryic acetates active compound, 4g Ufoxane 3A (lignosulfonates), 4g
EFW (alkylnaphthalene sulfonate and anion humectant mixture), 4g white carbons, 10g ammonium sulfate, 10g cornstarch, kaolin add
To 100g.It is uniformly mixed, after air-flow crushing, is granulated in comminutor, then dry, sieve up to granular product.
Embodiment 4：22% oxazole 2-cyano-3-amino-3-phenylancryic acetate aqueous emulsion
Weigh 20g oxazole active compound, 2g 2-cyano-3-amino-3-phenylancryic acetates active compound, 3.5g SK-5050 (ingredients：Polyoxyethylene aryl ether),
4.8g SK-551 (ingredients：Styrene phenol polyoxyethylene anionic dispersing agents), 806 synergist (Silwet of 2gSilwet
Ingredient is organic silicon), 0.18g xanthans, 5g ethylene glycol, 0.3g benzoic acid, 0.5g antifoaming agent (ingredient be organically-modified silica
Alkane), deionized water adds to 100g.Above-mentioned raw materials are added to after shearing 10min in high-shear emulsion machine, obtain aqueous emulsion.
Embodiment 5：16% oxazole 2-cyano-3-amino-3-phenylancryic acetate soluble powder
Weigh 15g oxazole active compound, 1g 2-cyano-3-amino-3-phenylancryic acetates active compound, 6g alkyl benzene calcium sulfonates, 12g ammonium sulfate, 2g alkylnaphthalenes
Sulfonate and anion humectant mixture, 0.3g sodium benzoates and cornstarch polishing to 100g.It is uniformly mixed, through air-flow powder
It after broken, is granulated in comminutor, then dries, sieves up to granular product.
Embodiment 6：21% oxazole 2-cyano-3-amino-3-phenylancryic acetate microemulsion
Weigh 20g oxazole active compound, 1g 2-cyano-3-amino-3-phenylancryic acetate active compounds, 7g N-Methyl pyrrolidones, 18g isopropanols, 4g agriculture breasts
600#, 6g agriculture breast 1601#, add water to complement to 100g.Active compound, solvent, emulsifier are added together, make to be dissolved into homogeneous oil phase；
Water-soluble component and water are mixed to prepare water phase；Under high velocity agitation, oil phase is mixed with water, it is preferable that physical and chemical performance is made
Microemulsion.
Embodiment 7：31% oxazole 2-cyano-3-amino-3-phenylancryic acetate suspending agent
Weigh 30g oxazole active compound, 1g 2-cyano-3-amino-3-phenylancryic acetates active compound, 8g FS3000 (Phosphate Anionic Surfactants),
2gTXC (sulfonate anionic surfactant), 0.2g xanthans, 1g aluminium-magnesium silicates, 1g ethylene glycol, 0.2g BIT (1,2- benzene
And isothiazoline -3- ketone), the organically-modified silica alkane antifoaming agent of 0.3g, deionized water adds to 100g.Above-mentioned raw materials are added to height
10min is sheared in emulsification pretreatment machine, then after being sanded with sand mill, obtain suspending agent.
Embodiment 8：41% oxazole 2-cyano-3-amino-3-phenylancryic acetate wettable powder
40g oxazole active compound, 1g 2-cyano-3-amino-3-phenylancryic acetate active compounds are weighed, 2g neopelexes, 8g white carbons, 8g are wooden
Plain sodium sulfonate, precipitated calcium carbonate complement to 100g.Above-mentioned raw materials are mixed, are crushed through Roughpulverizer, into airslide disintegrating mill powder
Broken mixing, obtains wettable powder.
Embodiment 9：61% oxazole 2-cyano-3-amino-3-phenylancryic acetate water dispersible granules
Weigh the sodium salt of 60g oxazole active compound, 1g 2-cyano-3-amino-3-phenylancryic acetates active compound, 4g alkyl naphthalene sulfonic acid condensation polymers, 4g ammonium sulfate,
4g alkylnaphthalene sulfonates and anion humectant mixture, 1g potassium sorbates and 10g cornstarch.It is uniformly mixed, through air-flow powder
It after broken, is granulated in comminutor, then dries, sieves up to granular product.
Embodiment 10：9.1% oxazole 2-cyano-3-amino-3-phenylancryic acetate microemulsion
Weigh 9g oxazole active compound, 0.1g 2-cyano-3-amino-3-phenylancryic acetate active compounds, 5g N-Methyl pyrrolidones, 10g isopropanols, 4g agricultures
Newborn 600#, 6g agriculture breast 1601#, adds water to complement to 100g.Active compound, solvent, emulsifier are added together, make to be dissolved into uniform oil
Phase；Water-soluble component and water are mixed to prepare water phase；Under high velocity agitation, oil phase is mixed with water, be made physical and chemical performance compared with
Good microemulsion.
Embodiment 11：5% oxazole 2-cyano-3-amino-3-phenylancryic acetate aqua
Weigh 2.5g oxazole active compound, 2.5g 2-cyano-3-amino-3-phenylancryic acetate active compounds, 6g phenethyl phenol polyethenoxy ethers condensation product, 0.3g
Sodium benzoate, water complement to 100g.Above-mentioned raw materials are added to blender to be uniformly mixed, you can obtain aqua.
Embodiment 12：10% oxazole 2-cyano-3-amino-3-phenylancryic acetate microemulsion
Weigh 2.5g oxazole active compound, 7.5g 2-cyano-3-amino-3-phenylancryic acetate active compounds, 6g N-Methyl pyrrolidones, 15g isopropanols, 4g
Agriculture breast 600#, 6g agriculture breast 1601#, adds water to complement to 100g.Active compound, solvent, emulsifier are added together, make to be dissolved into uniform oil
Phase；Water-soluble component and water are mixed to prepare water phase；Under high velocity agitation, oil phase is mixed with water, be made physical and chemical performance compared with
Good microemulsion.
Embodiment 13：30% oxazole 2-cyano-3-amino-3-phenylancryic acetate wettable powder
Weigh 5g oxazole active compound, 25g 2-cyano-3-amino-3-phenylancryic acetate active compounds, 1g neopelexes, 10g white carbons, 10g wood
Quality sodium sulfonate, precipitated calcium carbonate complement to 100g.Above-mentioned raw materials are mixed, are crushed through Roughpulverizer, into airslide disintegrating mill
It is pulverized and mixed, obtains wettable powder.
Embodiment 14：55% oxazole 2-cyano-3-amino-3-phenylancryic acetate water dispersible granules
Weigh 5g oxazole active compound, 50g 2-cyano-3-amino-3-phenylancryic acetates active compound, 3g Ufoxane 3A (lignosulfonates), 3g EFW
(alkylnaphthalene sulfonate and anion humectant mixture), 3g white carbons, 15g ammonium sulfate, 10g cornstarch, kaolin add to
100g.It is uniformly mixed, after air-flow crushing, is granulated in comminutor, then dry, sieve up to granular product.
Embodiment 15：80% oxazole 2-cyano-3-amino-3-phenylancryic acetate water dispersible granules
Weigh the sodium salt of 5g oxazole active compound, 75g 2-cyano-3-amino-3-phenylancryic acetates active compound, 2g alkyl naphthalene sulfonic acid condensation polymers, 5g ammonium sulfate,
2g alkylnaphthalene sulfonates and anion humectant mixture, 1g potassium sorbates and 10g cornstarch.It is uniformly mixed, through air-flow powder
It after broken, is granulated in comminutor, then dries, sieves up to granular product.
Embodiment 16：21% oxazole 2-cyano-3-amino-3-phenylancryic acetate seed treatment microcapsule suspending agent
Weigh 1% oxazole active compound, 20% 2-cyano-3-amino-3-phenylancryic acetate active compound, Ultrazine NA 1%, Sodium Polyacrylate 2%,
Polyureas performed polymer 10%, styrene maleic anhydride copolymer 1%, dimethylbenzene 15%, hydrochloric acid 3%, sodium hydroxide 0.01%,
Xanthans 0.01%, dispersible pigment color paste 3%, polyvinyl alcohol 2%, water complements to 100%.Active ingredient is dissolved in xylene solvent
In, obtain uniform oil-phase solution；Aqueous solution containing emulsified dispersant is added thereto, with being vigorously stirred, makes with solid
Body active compound, dimethylbenzene are oil phase, using aqueous solution containing emulsified dispersant as the O/W type stable emulsions of water phase.It will prepare in advance
Urea-formaldehyde prepolymer be added lotion in, using low content hydrochloric acid regulating system pH value, polymerize under acid catalysed conditions
Reaction, makes oil phase substance be wrapped, and forms microcapsule suspending agent.Into this microcapsule suspending agent be added thickener, antifreeze, at
Film, alert toner, stir evenly, both obtain seed treatment microcapsule suspending agent.
Embodiment 17：62% oxazole 2-cyano-3-amino-3-phenylancryic acetate seed treatment dispersible pulvis
Weigh 2g oxazole active compound, 60g 2-cyano-3-amino-3-phenylancryic acetates active compound, NNO 5%, pigment toner 3%, white carbon 5% is wooden
Plain sodium sulfonate 3%, lauryl sodium sulfate 2%, kaolin complements to 100%.According to recipe requirements, active ingredient is disperseed
Agent, alert toner, carrier premixing are uniform, then crush, and are then uniformly mixed, and Con trolling index detects at any time, discharges after qualified,
Packing is to get seed treatment dispersible pulvis.
Embodiment 18：41% oxazole 2-cyano-3-amino-3-phenylancryic acetate suspension seed-coating agent
Weigh 1g oxazole active compound, 40g 2-cyano-3-amino-3-phenylancryic acetate active compounds, dispersant A (virtue Wei Shiweike companies) 1g, Morwet
EFW 1g, Morwet D4251g, 600#1g, xanthans 0.2g, aluminium-magnesium silicate 2g, pigment toner 2g, polyvinyl alcohol 2g, water are mended
Enough to 100g.By active ingredient, wetting dispersing agent, emulsifier, thickener, the mixing such as toner, film forming agent, decentralized medium are warned, into
Row high shear is pre-dispersed, is then added in sand milling kettle, is ground to grain size and is less than 5 μm, you can suspension seed-coating agent is made.
Embodiment 19：61% oxazole 2-cyano-3-amino-3-phenylancryic acetate water dispersible granules
Weigh the sodium salt of 1g oxazole active compound, 60g 2-cyano-3-amino-3-phenylancryic acetates active compound, 4g alkyl naphthalene sulfonic acid condensation polymers, 4g ammonium sulfate,
4g alkylnaphthalene sulfonates and anion humectant mixture, 1g potassium sorbates and 10g cornstarch.It is uniformly mixed, through air-flow powder
It after broken, is granulated in comminutor, then dries, sieves up to granular product.
Embodiment 20：9.1% oxazole 2-cyano-3-amino-3-phenylancryic acetate microemulsion
Weigh 0.1g oxazole active compound, 9g 2-cyano-3-amino-3-phenylancryic acetate active compounds, 5g N-Methyl pyrrolidones, 10g isopropanols, 4g agricultures
Newborn 600#, 6g agriculture breast 1601#, adds water to complement to 100g.Active compound, solvent, emulsifier are added together, make to be dissolved into uniform oil
Phase；Water-soluble component and water are mixed to prepare water phase；Under high velocity agitation, oil phase is mixed with water, be made physical and chemical performance compared with
Good microemulsion.
1. The Toxicity Determination result：
Inventor carries out oxazole and 2-cyano-3-amino-3-phenylancryic acetate mixture to combine poison to watermelon blight germ (Fusarium oxysporum)
Power is tested, and specific test situation and result are as follows：
Using mycelial growth rate method.Under sterile conditions, with the card punch of a diameter of 5mm in the sharp spore sickle for cultivating 5d
Knife bacterium colony edge is beatened to take bacteria cake, and is placed in a series of PDA culture medium containing mass concentration gradient medicaments, is used in combination and sterile water is added
PDA culture medium as a contrast, each handle 3 repetitions, then by it is each processing be positioned in 25 DEG C of insulating boxs, culture 7d it is laggard
Row result is investigated.
It measures the colony diameter often handled when investigation respectively using crossing method, then calculates bacteriostasis rate.
Bacteriostasis rate (%)=[(control colony diameter-original bacteria cake diameter)-(processing colony diameter-original bacteria cake diameter)]/
[(control colony diameter-original bacteria cake diameter)] × 100
According to bacteriostasis rate, virulence regression equation and EC are found out50Value is calculated each by Sun-Johnson toxicity index calculating methods
Match the co-toxicity coefficient of mixture.
1. oxazole of table is with 2-cyano-3-amino-3-phenylancryic acetate composition to the indoor co-toxicity of watermelon blight germ (Fusarium oxysporum)
Measurement result
Table 1 the result shows that, different ratio shows synergistic effect, wherein with oxazole:2-cyano-3-amino-3-phenylancryic acetate is 20:1-1:
It is better when 20.Illustrate that oxazole has reasonability and feasibility with 2-cyano-3-amino-3-phenylancryic acetate mixture prevention watermelon blight.
2. field test results：
2.1 different embodiments prevent watermelon blight test result：
2.1.1 test material and method：
Experiment is located at the Shaoxing, Zhejiang Province city towns Gao Bu, and topography is flat, and irrigation and drainage are convenient.It is watermelon for studying object, kind is
8424,
Plot area 20m2.Experiment sets 15 processing altogether, is compareed containing clear water, often handles 3 repetitions, cell random alignment.
2.1.2 investigation method, time and number：
Start the 1st medication in watermelon blight initial phase, formulation rate is 250ml/ plant, interval the 2nd medication of 7d, the
7d investigates whether all plant of each cell have the classical symptom of droop, record withered respectively before 2 medications, after the 2nd medication
Disease morbidity strain number and investigation total strain number, finally calculate preventive effect.
2.1.3 drug effect computational methods:
Diseased plant rate (%)=diseased plant number/total strain number × 100
Control effect (%)=(1- treatment regions diseased plant number/blank district diseased plant number) × 100
2.1.4 to the other influences of crop
Whether observation crop has poisoning generation.
2.1.5 test result and analysis
The different embodiments of table 2. prevent watermelon blight test result
2-cyano-3-amino-3-phenylancryic acetate single dose is better than oxazole single dose to the preventive effect of watermelon blight as shown in Table 2, and passes through compounding
Preventive effect compared to two single dose 25% oxazole missible oil (oneself of the 12 listed embodiments above-mentioned later to watermelon blight
System) and 25% 2-cyano-3-amino-3-phenylancryic acetate suspending agent (commercially available) all increase, wherein proportioning be 1：20-20:The preventive effect of 1 embodiment has aobvious
It writes and is promoted, illustrate oxazole：2-cyano-3-amino-3-phenylancryic acetate=1:20—20:It is ideal to the preventive effect of watermelon blight in 1 range, and table
Reveal apparent synergistic effect.Field is estimated, and within the range of experimental dose, plant growth is normal, and each treatment agent does not occur
To the poisoning phenomenon of watermelon.
2.2 different embodiments prevent gray mold of cucumber test result：
2.2.1 test material and method：
Experiment is located at city of Zhangzhou City of Fujian Province Tian Bao town, and topography is flat, and irrigation and drainage are convenient.It is cucumber for examination vegetables, kind is
"Jinyou No.41", plot area 20m2.Experiment sets 15 processing altogether, is compareed containing clear water, often handles 3 repetitions, cell random alignment.
2.2.2 investigation method, time and number：
First meeting gray mold of cucumber spot starts medication, and sprayed a medicine every 7 days, sprays 2 times altogether.State of an illness radix is investigated before dispenser,
5 days after second of dispenser, investigate the state of an illness within 10 days.Take 5 points of investigation at random per cell, investigate 2 plants, every plant of investigation at every
Whole fruits are investigated and are classified and record in sampling point that a situation arises for gray mold in whole cucumber fruits.
Disease fruit classification uses following standard (as unit of fruit)：
0 grade：Disease-free spot；
1 grade：Residual flower morbidity；
3 grades：It falls ill in areola portion；
5 grades：Scab length accounts for 10% or less fruit；
7 grades：Scab length accounts for 11-the 25% of fruit；
9 grades：Scab length accounts for 26% of fruit or more；
2.2.3 drug effect computational methods:
In formula:Disease refers to before CK0-blank district dispenser；Disease refers to after CK1-blank district dispenser；
Disease refers to before PT0-chemicals treatment district dispenser；Disease refers to after PT1-chemicals treatment district dispenser.
2.2.4 to the other influences of crop
Whether observation crop has poisoning generation, should also record other beneficial effects to crop.
2.2.5 test result and analysis
The different embodiments of table 3. prevent gray mold of cucumber test result
Oxazole is higher than 2-cyano-3-amino-3-phenylancryic acetate to the preventive effect of gray mold of cucumber as shown in Table 3, according to above-mentioned listed implementation
Example test result finds out the two by 25% oxazole missible oil (self-control) of compared to two single doses of preventive effect after compounding and 25% cyanogen
Alkene bacterium ester suspending agent (commercially available) all increases, wherein proportioning is 1:20-20:1 embodiment preventive effect is obviously improved, and illustrates pyridine
Jun oxazoles are matched with 2-cyano-3-amino-3-phenylancryic acetate 20:1—1:There is synergistic effect to gray mold of cucumber when in 20 ranges.Field is estimated, examination
It tests in dosage range, plant growth is normal.
2.3 different embodiments prevent leaf muld of tomato test result：
2.3.1 test material and method：
Experiment is located at the Fujian Province towns Putian City Guo Huan, and topography is flat, and irrigation and drainage are convenient.It is tomato for examination vegetables, kind is Zhejiang
Powder 202, plot area 20m2.Experiment sets 15 processing altogether, is compareed containing clear water, often handles 3 repetitions, cell random alignment.
2.3.2 investigation method, time and number：
Leaf muld of tomato spot early period of origination first time medication, second of dispenser after 7 days, altogether dispenser 2 times.Disease is investigated before dispenser
Feelings radix, 7 days after first time dispenser, 7 days investigation state of an illness after second of dispenser.Take 5 points of investigation, every investigation 2 at random per cell
Strain, every plant point of upper, middle and lower part is taken a representational compound leaf, is accounted for every vanelets lesion area on compound leaf whole
The classification of a vanelets area percentage is investigated and is classified and records in sampling point that a situation arises for leaf mold on whole tomato leafs.
0 grade：Disease-free spot；
1 grade：Lesion area accounts for 5% or less blade area；
3 grades：Lesion area accounts for the 6%-10% of blade area；
5 grades：Lesion area accounts for the 11%-25% of blade area；
7 grades：Lesion area accounts for the 26%-50% of blade area；
9 grades：The percentage that lesion area accounts for blade area is more than 50%.
2.3.3 drug effect computational methods:
In formula:CK0Disease refers to before blank district dispenser；CK1Disease refers to after blank district dispenser；
PT0Disease refers to before chemicals treatment district dispenser；PT1Disease refers to after chemicals treatment district dispenser.
2.3.4 to the other influences of crop
Whether observation crop has poisoning generation, should also record other beneficial effects to crop.
2.3.5 test result and analysis
The different embodiments of table 4. prevent leaf muld of tomato test result
As can be seen from Table 4, the effect of oxazole prevention leaf muld of tomato is better than 2-cyano-3-amino-3-phenylancryic acetate.Wherein embodiment 3,
4, the 25% oxazole missible oil (self-control) of compared to two single doses of preventive effect and 25% cyanogen of 6,11,12,14 and 16 7 embodiments
Alkene bacterium ester suspending agent (commercially available) is obviously improved.Illustrate oxazole：2-cyano-3-amino-3-phenylancryic acetate=20:1—1:To tomato when in 20 ranges
Leaf mold synergistic effect is apparent.Field is estimated, and each processing does not occur to tomato poisoning phenomenon.
2.4 different embodiments prevent wheat scab test result：
2.4.1 test material and method：
Experimental plot is located at Shaoxing, Zhejiang Province city East Lake town Longshan village, and experimental field water and fertilizer condition is preferable, cultivation management condition one
It causes, Pass Test requirement.It is wheat for studying object, kind is Yangmai No.158, plot area 60m2.Experiment sets 15 processing altogether, contains
Clear water compares, and often handles 3 repetitions, cell random alignment.
2.4.2 investigation method, time and number：
In wheat flowering initial stage (about 20%) medication, interval sprays a medicine, continuous dispenser 2 times in 6 days.Every 667 square metres
It is watered 30L, carries out cauline leaf spraying, clear water is sprayed in blank control, and other water and fertilizer managements are consistent with surrounding crop field with insect pest preventing and controlling.
Incidence is managed in (after secondary medicine 25 days) investigation investigation everywhere after wheat scab stable disease.Treatment region is diagonal
5 points of samplings of line, every 50 fringes of investigation, record incidence；According to site of pathological change and hazard rating situation, disease index, meter are calculated
Calculate preventive effect.
2.4.3 drug effect computational methods:
Severity Scaling standard is as follows：
0 grade：Disease-free spot；
1 grade：Sick fringe area accounts for 1/4 or less full fringe area；
2 grades:Sick fringe area accounts for the 1/4~1/2 of full fringe area；
3 grades：Sick fringe area accounts for the 1/2~3/4 of full fringe area；
4 grades：Sick fringe area accounts for 3/4 or more of full fringe area；
Diseased plant rate (%)=diseased plant number/investigation strain number × 100
2.4.4 to the other influences of crop
Observing field diseases, a situation arises and wheat growth situation, observes the influence to Growth of Wheat, plant height, leaf color
Deng whether investigation crop has poisoning, and records poisoning type and extent of injury.
2.4.5 test result and analysis
The different embodiments of table 5. prevent wheat scab test result
2-cyano-3-amino-3-phenylancryic acetate is higher than oxazole to the preventive effect of wheat scab as shown in Table 5, and to small after the two compounding
The preventive effect of wheat head blight is higher than two 25% oxazole missible oil (self-control) of single dose and 25% 2-cyano-3-amino-3-phenylancryic acetate suspending agent (commercially available)
Preventive effect.Wherein, the preventive effect of six embodiments of embodiment 3,4,6,11,12,14 improves the most apparent, illustrates oxazole：
2-cyano-3-amino-3-phenylancryic acetate=20:1—1:It is ideal to the preventive effect of wheat scab when in 20 ranges.It is estimated according to field, in experiment agent
It measures in range, plant growth is normal, and each treatment agent does not occur the poisoning phenomenon to wheat, illustrates that it is safety to wheat
's.Therefore, oxazole is feasible and rational in the prevention of wheat scab with 2-cyano-3-amino-3-phenylancryic acetate composite usage and has apparent
Synergistic effect.
In conclusion the present invention contains the bactericidal composition of oxazole and 2-cyano-3-amino-3-phenylancryic acetate, to watermelon blight, cucumber ash
Mildew, leaf muld of tomato and wheat scab have preferable control effect, and it is safe to Target crops.Oxazole：Cyanogen
Alkene bacterium ester=90:1—1:There is synergistic effect to above-mentioned listed plant disease in 90 ranges, wherein most preferred compound proportion
It is 20:1—1:20.Complex preparation not only increases preventive effect, and expands fungicidal spectrum, widens use scope, reduces cost, right
Multiple diseases play the role of that a medicine is simultaneous to be controlled, and mitigate manpower and materials, improve productivity effect.So the invention of this complex preparation with
It promotes and disease control and crop yield raising is had a very important significance.
Claims (8)
1. a kind of bactericidal composition, it is characterised in that：The composition contains active constituent oxazole and 2-cyano-3-amino-3-phenylancryic acetate, pyridine bacterium
The weight part ratio of oxazole and 2-cyano-3-amino-3-phenylancryic acetate is 1:60-60:1, wherein weight part ratio is 60:Except 1.
2. bactericidal composition according to claim 1, which is characterized in that the weight part ratio of oxazole and 2-cyano-3-amino-3-phenylancryic acetate
It is 1:20-20:1.
3. bactericidal composition according to claim 1 or 2, which is characterized in that oxazole and the weight of 2-cyano-3-amino-3-phenylancryic acetate are total
With the 1%-80% for accounting for composition gross weight.
4. bactericidal composition according to claim 3, which is characterized in that oxazole and the weight summation of 2-cyano-3-amino-3-phenylancryic acetate account for
The 5%-60% of composition gross weight.
5. according to the bactericidal composition described in claim 1, which is characterized in that the composition is aqueous suspension agent, dispersible
Oil-suspending agent, aqueous emulsion, microemulsion, wettable powder, water dispersible granules, seed treatment dispersant, suspension seed-coating agent, at seed
Manage microcapsule suspending agent.
6. the purposes of the bactericidal composition described in a kind of any one of claim 1-5, which is characterized in that the composition is used
In prevention graw mold of tomato, gray mold of cucumber, leaf muld of tomato, alternaria leaf spot of apple, wheat scab, cotton wilt,
Banana Panama disease, bakanae disease of rice, watermelon blight.
7. the composition as described in any one of claim 1-5 apply Cereal, fruit tree and vegetable crop planting area with
Purposes in terms of control of plant disease caused by prevention sac fungus, basidiomycetes and Fungi Imperfecti.
8. purposes according to claim 7, which is characterized in that the component of the active constituent including the composition to be made
Preparation is applied, or is applied after the component bucket of the active constituent including the composition is mixed.
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| CN112823630B (en) * | 2019-11-21 | 2021-11-23 | 沈阳中化农药化工研发有限公司 | Seed treatment agent for sterilization |
Citations (4)
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|---|---|---|---|---|
| CN1280767A (en) * | 1999-07-14 | 2001-01-24 | 沈阳化工研究院 | Heterocycle substituted isoxazoline compounds used as disinfectant |
| CN1317483A (en) * | 2001-05-08 | 2001-10-17 | 江苏省农药研究所 | 2-cyano-3-substituted phenylacrylate compounds, compositions and their preparing prcess and application as disinfectant of agricultural crops |
| US20090048309A1 (en) * | 2005-02-06 | 2009-02-19 | Shenyang Research Institute Of Chemical Industry | N-(2-substituted phenyl)-n-methoxycarbamates and their preparation and use thereof |
| WO2011147953A1 (en) * | 2010-05-28 | 2011-12-01 | Basf Se | Pesticidal mixtures |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1280767A (en) * | 1999-07-14 | 2001-01-24 | 沈阳化工研究院 | Heterocycle substituted isoxazoline compounds used as disinfectant |
| CN1317483A (en) * | 2001-05-08 | 2001-10-17 | 江苏省农药研究所 | 2-cyano-3-substituted phenylacrylate compounds, compositions and their preparing prcess and application as disinfectant of agricultural crops |
| US20090048309A1 (en) * | 2005-02-06 | 2009-02-19 | Shenyang Research Institute Of Chemical Industry | N-(2-substituted phenyl)-n-methoxycarbamates and their preparation and use thereof |
| WO2011147953A1 (en) * | 2010-05-28 | 2011-12-01 | Basf Se | Pesticidal mixtures |
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Effective date of registration: 20230831 Address after: No. 88, Mount Taishan Street, Huludao Economic Development Zone, Liaoning Province 125003 Patentee after: Liaoning Youchuang Plant Protection Co.,Ltd. Address before: 17-19/F, New Shanghai City Plaza, No. 33 Henan South Road, Huangpu District, Shanghai, 200002 Patentee before: SINOCHEM AGRO Co.,Ltd. |