CN105340888B - A kind of bactericidal composition containing nitre benzene bacterium ester and cyflufenamid - Google Patents
A kind of bactericidal composition containing nitre benzene bacterium ester and cyflufenamid Download PDFInfo
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- CN105340888B CN105340888B CN201510938797.4A CN201510938797A CN105340888B CN 105340888 B CN105340888 B CN 105340888B CN 201510938797 A CN201510938797 A CN 201510938797A CN 105340888 B CN105340888 B CN 105340888B
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- cyflufenamid
- bacterium ester
- powdery mildew
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- benzene bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/52—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing groups, e.g. carboxylic acid amidines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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Abstract
The invention discloses bactericidal composition and its application of a kind of bacterium ester of benzene containing nitre and cyflufenamid, effective active composition is made up of the first active component nitre benzene bacterium ester and the second active component cyflufenamid, and the weight ratio of the first active component and the second active component is 1：50~50：1, preferably 1：1~40：1, more preferably 5：1~36：1.Suspending agent that this composition can be configured to agriculturally to allow, microemulsion, aqueous emulsion, aqua, dispersible oil-suspending agent, wettable powder, dosage form of water dispersible granule.Rationally, bactericidal effect is good for instant component, and its activity and bactericidal effect are not the simple superposition of each component activity, compared with existing unitary agent, in addition to significant bactericidal effect, and have significant synergistic effect, good to crop safety.The present invention has significant prevention effect to crop powdery mildew.
Description
Technical field
The present invention relates to a kind of bactericidal composition, by the first active component nitre benzene bacterium ester and the second active component cyflufenamid
And auxiliary agent composition, belong to technical field of compounded pesticide.
Background technology
Nitre benzene bacterium ester, English name meptyldinocap, chemical name：2,4- dinitros -6- (1- methylheptyls) benzene crotons
Acid esters.The methoxy acrylic bactericide that nitre benzene bacterium ester is developed by The Dow Agrosciences, LLC., is from sterilization（Mite）Agent is opposed
Mite general (dinocap, CAS:39300-45-3) the isomers with highest bioactivity separated in 6 kinds of isomers
One of.The new product more friendly to environment and non-target organism, mechanism of action is consistent with dinocap.It is a kind of cause of disease oxidation
The uncoupler of phosphorylation, with prevention, treatment and shoveling function.It is non-internal-absorting acaricide, while having certain contact
Property bactericidal action.The red spider and powdery mildew for being mainly used in preventing and treating on wheat, fruit tree, vegetables, flowers and the crop such as melon it is anti-
Control, have good prevention effect to mulberry tree powdery mildew and eggplant red spider, while the also effect with mite killing ovum.Nitre benzene bacterium ester
Be lipophilic compound, may act on multiple sites, plant leaf blade surface can rapidly and blade surface wax coat knot
Close, resistance to rain leaching, with the lasting period it is long the features such as.
Cyflufenamid, English name cyflufenamid, chemical name：(Z)-N- [α-(methoxyimino of ring third) -2,3- bis-
Fluoro- 6- (trifluoromethyl) benzyl] -2- phenyl acetamides are to be developed for preventing and treating various crop powdery mildews by Japanese Cao Da companies
Acid amide fungicides.The cyflufenamid mechanism of action be can effectively suppress the haustorium on Powdery Mildew mycelia formation and growth,
The growth of secondary hyphae and conidial formation.It is not sterol, phosphatide, chitin and protein synthesis inhibitor, not shadow
Ring the function of mitochondrial respiratory and cell membrane.With excellent protection, therapeutic action, holding effect activity and resistance of rainwater washing against are active.
The mechanism of action in terms of biochemistry is not clear, under study for action.Cyflufenamid and benzimidazole, triazole type and methoxy propyl
Numerous bactericide no interactions resistances such as olefin(e) acid esters.Influence of the cyflufenamid to the spore-germination and hyphal elongation of pathogen also with
The form of other existing medicaments is different.
Powdery mildew is widely distributed in China, and the harm to dicotyledon is especially prevalent.After powdery mildew occurs, host is aggrieved
Position growth is suppressed, gradually moves back green flavescence, and it is dried-up to last full leaf withered spot occur, makes plant early withered when serious.The disease later stage,
In the mould layer of white powder shape be made up of mycelia and conidium, often there is the granular cleistothecium of many scattered black, and get over this
Winter, secondary spring carries out First aggression with ascospore.Powdery mildew is once occur, and spread speed is very fast, one season of growth of crops
Can be repeatedly subjected to infect.Powdery mildew makes the crop products such as China's wheat, cucumber, grape be subjected to extreme loss, therefore, prevent
The generation for controlling powdery mildew is significant to agricultural production.
With the passage and the continuous increase of usage amount of use time, pathogen produces anti-to nitre benzene bacterium ester and cyflufenamid
Property be a kind of inevitable.There is and expand preventing and treating spectrum to slow down resistance, bactericide is mixed to be very important.Applicant through retrieval,
There is presently no the document report that nitre benzene bacterium ester and cyflufenamid mixture prevent and treat powdery mildew, it is further advanced by experiment and finds, will
The different nitre benzene bacterium ester of the mechanism of action and cyflufenamid are compounded, and tool has an unexpected effect, and not only synergistic effect is notable, moreover it is possible to
Significantly improve the prevention effect to powdery mildew, reduce dosage, cost accounting is reduced, to crop safety.
The content of the invention
It is an object of the invention to provide a kind of component rationally, significantly, bactericidal effect is good, and drug cost is low for synergistic effect,
To the agricultural bactericidal composition of crop safety.
Bactericidal composition preparation formulation another object of the present invention is to provide the bacterium ester of benzene containing nitre and cyflufenamid.
It is still another object of the present invention to provide application of the above-mentioned composition on preventing and treating crop powdery mildew.
In order to overcome the defect of existing unitary agent, the technical scheme is that so solve：
A）First active component nitre benzene bacterium ester；
B）Second active component cyflufenamid；
The weight ratio of the first active component and the second active component is 1：50~50：1, preferably 1：1~40：1, further
Preferably 5：1~36：1.
A kind of bactericidal composition containing nitre benzene bacterium ester and cyflufenamid of the present invention is according to those skilled in the art of the present technique institute
The preparation formulation that known method can be prepared is suspending agent, microemulsion, aqueous emulsion, aqua, dispersibles oil-suspending agent, wettable
Pulvis, water dispersible granules.
To suspending agent, usable auxiliary agent has：Dispersant such as polycarboxylate, lignosulfonates, alkyl naphthalene formaldehyde condensation
Thing sulfonate, alkylphenol-polyethenoxy base ether phosphate, alkylphenol-polyethenoxy base ether sulfonate, phenethyl phenol polyoxyethylene groups
Ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, pull open powder, dodecyl
One or more in polyoxyethylene ether phosphate etc.；Wetting agent such as alkylphenol-polyethenoxy base ether formaldehyde condensation products sulfate,
Phenethyl phenol polyoxyethylene groups ether phosphate, alkyl sulfate, alkylsulfonate, alkylnaphthalene sulfonate, alkylphenol-formaldehyde resin
One or more in APEO, in triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer etc.；Thickener is such as
In xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate, carboxymethylcellulose calcium, carboxyethyl cellulose, methylcellulose etc. one
Plant or various；One or more in preservative such as formaldehyde, benzoic acid, Sodium Benzoate, potassium sorbate, OIT；Disappear
Infusion such as silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 aliphatic alcohols, hexanol, butanol, octanol
One or more in；Antifreezing agent such as ethylene glycol, propane diols, glycerine, polyethylene glycol, sorbierite, urea, inorganic salts etc.
In one or more；Water is deionized water.
For microemulsion, usable auxiliary agent has：Emulsifying agent such as agriculture breast 500#（Calcium dodecyl benzene sulfonate）, agriculture breast
700#, agriculture breast 2201, this disk-60#, polysorbate60-#, TX-10, agriculture breast 1601（Phenethyl phenol polyoxyethylene poly-oxygen propylene aether）、
One or more in agriculture breast 600#, agriculture breast 400# etc.；One kind in assistant for emulsifying agent such as methyl alcohol, isopropanol, n-butanol, ethanol
Or it is various；Solvent such as cyclohexanone, 1-METHYLPYRROLIDONE, dimethylbenzene, toluene, solvent naphtha（The trade mark：S-150、S-180、S-200）
One or more in；One or more in stabilizer such as triphenyl phosphite, epoxychloropropane；Water is deionization
Water.
To aqueous emulsion, usable auxiliary agent has：Emulsifying agent such as Nonyl pheno（EO=10）Ether phosphate, triphen second
Base phenol polyoxyethylene phosphonate, Nongru-700 #（Alkyl phenol formaldehyde resin polyoxyethylene ether）, agriculture breast 2201#, this disk -60#
（Span60）, Tween-60 #（Polyoxyethylene sorbitan stearate）、TX-10（Octyl phenol polyoxy second
Alkene（10）Ether）, agriculture breast 1601（Triphenoethyl benzene phenol polyoxyethylene polyoxypropylene block polymer）, agriculture breast 600#, agriculture breast 400#
One or more in；Solvent such as dimethylbenzene, toluene, cyclohexanone, solvent naphtha（The trade mark：S-150、S-180、S-200）In
One or more；One or more in stabilizer such as triphenyl phosphite, epoxychloropropane, epoxidized soybean oil；It is antifreeze
One or more in agent such as ethylene glycol, propane diols, glycerine, urea, inorganic salts such as sodium chloride；Thickener such as xanthans,
One or more in polyvinyl alcohol, bentonite, aluminium-magnesium silicate etc.；In preservative such as formaldehyde, benzoic acid, Sodium Benzoate one
Plant or various, water is deionized water.
To aqua, usable auxiliary agent has：Emulsifying agent such as calcium dodecyl benzene sulfonate, alkylphenol-formaldehyde resin polyoxyethylene
Ether, agriculture breast 2201#, sorbitan monostearate, sorbitan monostearate APEO, octyl phenol polyoxyethylene
（10）Ether, AEO, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, agriculture breast 600#, agriculture breast 400#, dodecane
One or more in base dimethyl benzyl ammonium chloride etc.；Water is deionized water.
To dispersibling oil-suspending agent, usable auxiliary agent has：Dispersant such as polycarboxylate, lignosulfonates, alkylnaphthalene
Sulfonate（Dispersing agent NNO）、TERSPERSE 2020（Huntsman Corporation of U.S. HUNTSMAN produces, alkyl naphthalene sulfonic acid salt）Deng
In one or more；Emulsifying agent such as BY（Castor oil polyoxyethylene ether）Serial emulsifying agent（BY-110、BY-125、BY-140）、
Nongru-700 #（Alkyl phenol formaldehyde resin polyoxyethylene ether）, agriculture breast 2201, this disk -60#（Common name：Sorbitol anhydride monostearate
Ester）, Tween-60 #（Sorbitan monostearate APEO）, agriculture breast 1601#（Phenethyl phenol polyoxyethylene polyoxy
Propylene ether）、TERSPERSE 4894（Huntsman Corporation of the U.S. produces）One or more in；Wetting agent such as alkyl phenol polyoxy
Vinyl ethers formaldehyde condensation products sulfate, APES phosphate, phenethyl phenol polyoxyethylene groups ether phosphate, alkane
Base sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE 2500（Huntsman Corporation of the U.S. produces）One kind or many in
Kind；One or more in thickener such as white carbon, polyvinyl alcohol, bentonite, aluminium-magnesium silicate；Antifreezing agent such as ethylene glycol, third
One or more in glycol, glycerine, urea, inorganic salts such as sodium chloride；Decentralized medium such as soybean oil, rapeseed oil, corn
One or more in oil, methyl oleate, diesel oil, machine oil, mineral oil etc..
To wettable powder, usable auxiliary agent has：Dispersant such as polycarboxylate, lignosulfonates, polyoxyethylene gather
Oxypropylene group ether block copolymers, alkyl naphthalene formaldehyde condensate sulfonates, alkylphenol-polyethenoxy base ether phosphate, poly alkyl alcohol
One or more in oxygen ethene ether phosphate, alkylphenol-polyethenoxy base ether sulfonate etc.；Wetting agent such as alkyl sulfate, alkane
One or more in base sulfonate, alkylnaphthalene sulfonate etc.；Filler such as ammonium sulfate, urea, sucrose, glucose, diatomite, height
One or more in ridge soil, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay etc..
For water dispersible granules, usable auxiliary agent has：Dispersant such as polycarboxylate（TERSPERSE 2700、T36、GY-
D06 etc.）, lignosulfonates, one or more in alkylnaphthalene sulfonate etc.；Wetting agent such as alkyl sulfate, alkyl sulfonic acid
One or more in salt, naphthalene sulfonate etc.；Disintegrant such as ammonium sulfate, sodium sulphate, polyvinylpyrrolidone, starch and its derivative
One or more in thing, bentonite etc.；Binding agent such as starch, glucose, polyvinyl alcohol, polyethylene glycol, carboxymethylcellulose calcium
One or more in sodium, sucrose etc.；Filler such as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay
One or more in.
Rationally, treatment adds protective effect to instant component, and bactericidal effect is good, and drug cost is low, and its activity is imitated with sterilized
Fruit is not the simple superposition of each component activity, but has significant synergistic effect, good to crop safety, meets pesticidal preparations
Security requirement.The present invention has excellent prevention effect to crop powdery mildew.
Specific embodiment
Illustrate whether nitre benzene bacterium ester and cyflufenamid mixture have synergy to powdery mildew below by Toxicity Determination
Effect.
1st, the synergy to wheat powdery mildew mixture is determined.
In order to prevent and treat the wheat powdery mildew in agricultural production, we have carried out mutual compounding with nitre benzene bacterium ester and cyflufenamid
Synergy research, specific method is as follows.
Subjects are wheat powdery mildew（Erysiphe graminis）.The test medicine that active compound is configured to need,
Test method is referred to《The part of farm-chemical indoor determination test rule bactericide the 4th：Preventing and treating wheat powdery mildew experiment is potted plant
Method》With《The part of farm-chemical indoor determination test rule bactericide the 6th：The synergy of mixture is determined》.
Single dose and each Mixed Pharmacy are set into 5 various concentrations gradients first（On the basis of pilot study, bacteriostasis rate exists
Set by geometric progression in the range of 5%~90%）.If blank, it is repeated 4 times.Using pot-culture method, liquid is uniformly sprayed in
On wheat blade face, dry naturally.The Powdery Mildew Fresh spores produced in 24h on wheat leaf blade of falling ill uniformly are shaken off to be inoculated in
On 3 leaf phase potted plant wheat seedlings for the treatment of.3 basins are often processed, per 10 plants of basin.24h after chemicals treatment, is sprayed with spore suspension and connect
Kind, then natural air drying moves to thermostatic chamber, is 20~25 DEG C in temperature, and relative humidity is 70%~90% greenhouse.Treat blank pair
When reaching more than 80% according to sick leaf rate, incidence is managed in classification investigation everywhere, the whole blades of often treatment investigation.According to blank
Incidence classification investigation, using following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of full wafer leaf area；
3 grades：Lesion area accounts for the 6%~15% of full wafer leaf area；
5 grades：Lesion area accounts for the 16%~25% of full wafer leaf area；
7 grades：Lesion area accounts for the 26%~50% of full wafer leaf area；
9 grades：Lesion area accounts for more than the 50% of full wafer leaf area.
According to survey data, the disease index and prevention effect of each treatment are calculated.By preventive effect niqueMin and series concentration
Logarithm value between linear regression analysis, obtain the EC of each medicament50Value.
The co-toxicity coefficient of mixture is calculated with the abundant methods of Sun Yun（CTC）, activity of the reagent agent to germ is evaluated with this.Compounding
The co-toxicity coefficient of preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜ CTC ＜ 120 are showed
It is summation action.
The nitre benzene bacterium ester of table 1 and cyflufenamid combine the Toxicity Determination to wheat powdery mildew
| Chemicals treatment | ED50（mg/L） | ATI | TTI | CTC |
| Cyflufenamid（A） | 0.94 | 100.00 | —— | —— |
| Nitre benzene bacterium ester（B） | 0.89 | 105.62 | —— | —— |
| A:B=1:50 | 0.68 | 138.24 | 105.51 | 131.02 |
| A:B=1:40 | 0.59 | 159.32 | 105.48 | 151.04 |
| A:B=1:30 | 0.63 | 149.21 | 105.44 | 141.51 |
| A:B=1:20 | 0.57 | 164.91 | 105.35 | 156.54 |
| A:B=1:10 | 0.58 | 162.07 | 105.11 | 154.19 |
| A:B=1:1 | 0.55 | 170.91 | 102.81 | 166.24 |
| A:B=5:1 | 0.51 | 184.31 | 100.94 | 182.60 |
| A:B=10:1 | 0.48 | 195.83 | 100.51 | 194.84 |
| A:B=20:1 | 0.53 | 177.36 | 100.27 | 176.89 |
| A:B=30:1 | 0.51 | 184.31 | 100.18 | 183.98 |
| A:B=36:1 | 0.54 | 174.07 | 100.15 | 173.81 |
| A:B=40:1 | 0.57 | 164.91 | 100.14 | 164.69 |
| A:B=50:1 | 0.62 | 151.61 | 100.11 | 151.45 |
Toxicity Determination result shows：Nitre benzene bacterium ester and cyflufenamid with weight ratio be 1：50~50：1 is mixed to wheat
Powdery mildew has preferable virulence, and weight ratio is 1：1~40：Significantly, especially weight ratio is 5 for 1 synergistic effect：1~36：1 synergy
Effect is the most notable.
2nd, the synergy to powdery mildew of cucumber mixture is determined.
In order to prevent and treat the powdery mildew of cucumber in agricultural production, we have carried out mutual compounding with nitre benzene bacterium ester and cyflufenamid
Synergy research, specific method is as follows.
Subjects are cucumber powdery mildew's pathogen（Sphaerotheca fuliginea）.The experiment that active compound is configured to need
Medicament, test method reference《The part of farm-chemical indoor determination test rule bactericide the 6th：The synergy of mixture is determined》With
《The part of farm-chemical indoor determination test rule bactericide the 11st：Preventing and treating cucurbits powdery mildew experiment pot-culture method》.
Single dose and each Mixed Pharmacy are set into 5 various concentrations gradients first（On the basis of pilot study, bacteriostasis rate exists
Set by geometric progression in the range of 5%~90%）.If blank, it is repeated 4 times.Using pot-culture method, liquid is uniformly sprayed in
Blade face, after standby after liquid natural air drying.Spore suspension spray inoculation is used, then natural air drying moves to thermostatic chamber, in temperature
Under conditions of 20~24 DEG C, 7d~10d is cultivated.When blank disease leaf rate reaches more than 80%, classification investigation is got a haircut everywhere
State of an illness condition, often processes at least 30 leaves of investigation.Grade scale is：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 5%~15% of whole leaf area；
5 grades：Lesion area accounts for the 15%~25% of whole leaf area；
7 grades：Lesion area accounts for the 25%~50% of whole leaf area；
9 grades：Lesion area accounts for the 50%~75% of whole leaf area；
11 grades：Lesion area accounts for more than the 75% of whole leaf area；
According to survey data, the disease index and prevention effect of each treatment are calculated.By preventive effect niqueMin and series concentration
Logarithm value between linear regression analysis, obtain the EC50 values of each medicament.
The co-toxicity coefficient of mixture is calculated with the abundant methods of Sun Yun（CTC）, activity of the reagent agent to germ is evaluated with this.Compounding
The co-toxicity coefficient of preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜ CTC ＜ 120 are showed
It is summation action.
The nitre benzene bacterium ester of table 2 and cyflufenamid combine the Toxicity Determination to powdery mildew of cucumber
| Chemicals treatment | ED50（mg/L） | ATI | TTI | CTC |
| Cyflufenamid（A） | 1.05 | 100.00 | —— | —— |
| Nitre benzene bacterium ester（B） | 1.24 | 84.68 | —— | —— |
| A:B=1:50 | 0.83 | 126.51 | 84.98 | 148.87 |
| A:B=1:40 | 0.79 | 132.91 | 85.05 | 156.27 |
| A:B=1:30 | 0.84 | 125.00 | 85.17 | 146.76 |
| A:B=1:20 | 0.79 | 132.91 | 85.41 | 155.62 |
| A:B=1:10 | 0.77 | 136.36 | 86.07 | 158.43 |
| A:B=1:1 | 0.68 | 154.41 | 92.34 | 167.22 |
| A:B=5:1 | 0.59 | 177.97 | 97.45 | 182.63 |
| A:B=10:1 | 0.59 | 177.97 | 98.61 | 180.48 |
| A:B=20:1 | 0.56 | 187.50 | 99.27 | 188.88 |
| A:B=30:1 | 0.54 | 194.44 | 99.51 | 195.41 |
| A:B=36:1 | 0.56 | 187.50 | 99.59 | 188.28 |
| A:B=40:1 | 0.62 | 169.35 | 99.63 | 169.99 |
| A:B=50:1 | 0.73 | 143.84 | 99.70 | 144.27 |
Toxicity Determination result shows：Nitre benzene bacterium ester and cyflufenamid with weight ratio be 1：50~50：1 is mixed to cucumber
Powdery mildew has preferable virulence, and weight ratio is 1：1~40：Significantly, especially weight ratio is 5 for 1 synergistic effect：1~36：1 synergy
Effect is the most notable.
3rd, the synergy to powdery mildew of strawberry mixture is determined.
In order to prevent and treat the powdery mildew of cucumber in agricultural production, we have carried out mutual compounding with nitre benzene bacterium ester and cyflufenamid
Synergy research, specific method is as follows.
Subjects are strawberry powdery mildew（Sphaerotheca macularis）.The experiment that active compound is configured to need
Medicament, test method reference《The part of farm-chemical indoor determination test rule bactericide the 6th：The synergy of mixture is determined》With
《The part bactericidal agent for preventing and treating powdery mildew of strawberry of pesticide field efficacy medicine test criterion (two) the 119th》.
Single dose and each Mixed Pharmacy are set into 5 various concentrations gradients first（On the basis of pilot study, bacteriostasis rate exists
Set by geometric progression in the range of 5%~90%）.If blank, it is repeated 4 times, using pot-culture method, often processes 5 basins, per basin 5
Strain.Liquid is uniformly sprayed in blade face, after standby after liquid natural air drying.Use spore suspension spray inoculation, natural air drying, so
After move to thermostatic chamber, temperature be 20~24 DEG C under conditions of, cultivate 7d~10d.Treat that blank disease leaf rate reaches more than 80%
When, incidence is managed in classification investigation everywhere, and 3 plants, every plant of whole blades of investigation are chosen per basin.Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%~15% of whole leaf area；
5 grades：Lesion area accounts for the 16%~25% of whole leaf area；
7 grades：Lesion area accounts for the 26%~50% of whole leaf area；
9 grades：Lesion area accounts for more than the 51% of whole leaf area.
According to survey data, the disease index and prevention effect of each treatment are calculated.By preventive effect niqueMin and series concentration
Logarithm value between linear regression analysis, obtain the EC of each medicament50Value.
The co-toxicity coefficient of mixture is calculated with the abundant methods of Sun Yun（CTC）, activity of the reagent agent to germ is evaluated with this.Compounding
The co-toxicity coefficient of preparation（CTC）>=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜ CTC ＜ 120 are showed
It is summation action.
The nitre benzene bacterium ester of table 3 and cyflufenamid combine the Toxicity Determination to powdery mildew of strawberry
| Chemicals treatment | ED50（mg/L） | ATI | TTI | CTC |
| Cyflufenamid（A） | 0.78 | 100.00 | —— | —— |
| Nitre benzene bacterium ester（B） | 0.92 | 84.78 | —— | —— |
| A:B=1:50 | 0.66 | 118.18 | 85.08 | 138.91 |
| A:B=1:40 | 0.65 | 120.00 | 85.15 | 140.92 |
| A:B=1:30 | 0.58 | 134.48 | 85.27 | 157.71 |
| A:B=1:20 | 0.62 | 125.81 | 85.51 | 147.13 |
| A:B=1:10 | 0.59 | 132.20 | 86.17 | 153.43 |
| A:B=1:1 | 0.51 | 152.94 | 92.39 | 165.54 |
| A:B=5:1 | 0.41 | 190.24 | 97.46 | 195.19 |
| A:B=10:1 | 0.45 | 173.33 | 98.62 | 175.76 |
| A:B=20:1 | 0.42 | 185.71 | 99.28 | 187.07 |
| A:B=30:1 | 0.41 | 190.24 | 99.51 | 191.18 |
| A:B=36:1 | 0.43 | 181.40 | 99.59 | 182.14 |
| A:B=40:1 | 0.47 | 165.96 | 99.63 | 166.58 |
| A:B=50:1 | 0.53 | 147.17 | 99.70 | 147.61 |
Toxicity Determination result shows：Nitre benzene bacterium ester and cyflufenamid with weight ratio be 1：50~50：1 is mixed to strawberry
Powdery mildew has preferable virulence, and weight ratio is 1：1~40：Significantly, especially weight ratio is 5 for 1 synergistic effect：1~36：1 synergy
Effect is the most notable.
In order to the present invention is better described, present invention is described further with reference to embodiment, in formula
Percentage is weight percentage.
Example of formulations 1
Weigh 20% nitre benzene bacterium ester, 2% cyflufenamid, 6% sodium lignin sulfonate, 2% sodium naphthalene sulfonate, 5% sodium alkyl sulfate,
0.4% aluminium-magnesium silicate, 0.1% bentonite, 5% propane diols, 0.1% Sodium Benzoate, 1% hexanol, deionized water add to 100% weight
Part.Above-mentioned raw materials are blended, high speed shear dispersion 30min, are sanded to particle diameter D with sand mill9022% nitre is obtained after less than 10 μm
Benzene bacterium ester cyflufenamid suspending agent.
Example of formulations 2
Weigh 10% nitre benzene bacterium ester, 10% cyflufenamid, 3% polycarboxylate, 2% phenethyl phenol polyoxyethylene groups ether phosphate,
5% sodium alkyl sulfate, 0.5% carboxymethylcellulose calcium, 3% glycerine, 0.2% formaldehyde, 1% butanol, deionized water add to 100% weight
Part.Above-mentioned raw materials are blended, high speed shear dispersion 30min, are sanded to particle diameter D with sand mill9020% nitre benzene is obtained after less than 10 μm
Bacterium ester cyflufenamid suspending agent.
Example of formulations 3
Weigh 1% nitre benzene bacterium ester, 20% cyflufenamid, 3% alkyl naphthalene formaldehyde condensation product sodium sulfonate, 2% phenethyl phenol polyoxy second
Alkenyl ether phosphate, 5% sodium alkyl sulfate, 0.5% carboxyethyl cellulose, 3% polyethylene glycol, 0.2% potassium sorbate, 2% octanol, go
Ionized water adds to 100% weight portion.Above-mentioned raw materials are blended, high speed shear dispersion 30min, are sanded to particle diameter D with sand mill90It is small
In prepared 21% nitre benzene bacterium ester cyflufenamid suspending agent after 10 μm.
Example of formulations 4
Weigh 15% nitre benzene bacterium ester, 1% cyflufenamid, 4%TX-10,6% Nongru-700 #, 6% agriculture breast 500#, 4% agriculture breast 1601#,
15% cyclohexanone, 5% toluene, 5% n-butanol, through dissolving complete and being well mixed, deionized water adds to 100% weight portion, after stirring
16% nitre benzene bacterium ester cyflufenamid microemulsion is obtained.
Example of formulations 5
Weigh 1% nitre benzene bacterium ester, 10% cyflufenamid, 5% agriculture breast 400#, 4% agriculture breast 500#, 5% agriculture breast 1601#, 25% hexamethylene
Ketone, 5% dimethylbenzene, 5% ethanol, through dissolving complete and being well mixed, deionized water adds to 100% weight portion, and 11% is obtained after stirring
Nitre benzene bacterium ester cyflufenamid microemulsion.
Example of formulations 6
Weigh 1% nitre benzene bacterium ester, 15% cyflufenamid, 5% agriculture breast 400#, 3% agriculture breast 2201,6% agriculture breast 1601#, 25% solvent
Oily S-150,5% dimethylbenzene, 5% isobutanol, through dissolving complete and being well mixed, deionized water adds to 100% weight portion, after stirring
16% nitre benzene bacterium ester cyflufenamid microemulsion is obtained.
Example of formulations 7
Weigh 36% nitre benzene bacterium ester, 1% cyflufenamid, 3% agriculture breast 2201#, 2% Nonyl pheno（EO=10）Ether phosphorus
Acid esters, 2% Tween-60 #, 2% this disk -60#, 5% propane diols, 10% solvent naphtha S-200,5% cyclohexanone, 0.2% xanthans, 0.5%
Benzoic acid, deionized water adds to 100% weight portion.Above-mentioned raw materials are blended, and high speed shearing emulsification is obtained 37% nitre benzene bacterium ester ring
Fluorine bacterium aqueous amine emulsion.
Example of formulations 8
Weigh 1% nitre benzene bacterium ester, 25% cyflufenamid, 5% agriculture breast 1601,4% Nonyl pheno（EO=10）Ether phosphoric acid
Ester, 5% triphenoethyl benzene phenol polyethenoxy ether phosphate, 2% this disk -60#, 1% epoxychloropropane, 2% ethylene glycol, 5% dimethylbenzene,
0.2% xanthans, 0.5% Sodium Benzoate, deionized water add to 100% weight portion.Above-mentioned raw materials are blended, high speed shearing emulsification system
Obtain 26% nitre benzene bacterium ester cyflufenamid aqueous emulsion.
Example of formulations 9
1% nitre benzene bacterium ester, 35% cyflufenamid, 8% agriculture breast TX-10#, 2% agriculture breast 400#, 4% triphenoethyl benzene phenol is weighed to gather
Oxygen ethene ether phosphate, 2% this disk -60#, 1% epoxychloropropane, 2% ethylene glycol, 5% dimethylbenzene, 0.2% polyvinyl alcohol, 0.5% benzene
Formic acid, deionized water adds to 100% weight portion.Above-mentioned raw materials are blended, and high speed shearing emulsification is obtained 36% nitre benzene bacterium ester ring fluorine
Bacterium aqueous amine emulsion.
Example of formulations 10
20% nitre benzene bacterium ester, 1% cyflufenamid, 3%TX-10 are weighed, deionized water adds to 100% weight portion.Above-mentioned raw materials are passed through
Mixing, stirring and dissolving is obtained 21% nitre benzene bacterium ester cyflufenamid aqua afterwards completely.
Example of formulations 11
1% nitre benzene bacterium ester, 45% cyflufenamid, 0.5% agriculture breast 500#, 3% Nongru-700 # are weighed, deionized water adds to 100% weight
Amount part.Above-mentioned raw materials are blended, and stirring and dissolving is obtained 46% nitre benzene bacterium ester cyflufenamid aqua afterwards completely.
Example of formulations 12
30% nitre benzene bacterium ester, 3% cyflufenamid, 3% calcium dodecyl benzene sulfonate, 2% agriculture breast 400# are weighed, deionized water is added to
100% weight portion.Above-mentioned raw materials are blended, and stirring and dissolving is obtained 33% nitre benzene bacterium ester cyflufenamid aqua afterwards completely.
Example of formulations 13
Weigh 15% nitre benzene bacterium ester, 3% cyflufenamid, 2%TERSPERSE 2020（Huntsman Corporation of the U.S. produces）、4%
BY-140,3% Tween-60 #, 4% agriculture breast 2201#, 3% Nongru-700 #, 1% APES phosphate, 2% aluminium-magnesium silicate,
5% polyvinyl alcohol, soybean oil adds to 100% weight portion.Above-mentioned raw materials are blended, high speed shear dispersion 30min, are sanded with sand mill
After make 18% nitre benzene bacterium ester cyflufenamid and dispersible oil-suspending agent.
Example of formulations 14
Weigh 1% nitre benzene bacterium ester, 25% cyflufenamid, 2% sodium lignin sulfonate, 4% this disk -60#, 8% BY-110,1%
TERSPERSE 2500（Huntsman Corporation of the U.S. produces）, 1% bentonite, 3% glycerine, castor oil adds to 100% weight portion.It is above-mentioned
Raw material is blended, high speed shear dispersion 30min, 26% nitre benzene bacterium ester cyflufenamid is obtained after being sanded with sand mill and dispersibles oil
Suspending agent.
Example of formulations 15
Weigh 1% nitre benzene bacterium ester, 15% cyflufenamid, 2% dispersing agent NNO, 3% this disk -60#, 6% BY-125,1%T naphthalene sulfonic acids
Sodium, 1% bentonite, 3% propane diols, small wheat oil add to 100% weight portion.Above-mentioned raw materials are blended, high speed shear dispersion 30min, use
Sand mill is obtained 16% nitre benzene bacterium ester cyflufenamid and dispersibles oil-suspending agent after being sanded.
Example of formulations 16
Weigh 25% nitre benzene bacterium ester, 1% cyflufenamid, 4% polyoxyethylene polyoxypropylene base ether block copolymers, 3% wooden
Plain sodium sulfonate, 4% Negel, fine particle calcium carbonate are filled up to 100% weight portion.Above-mentioned raw materials are blended, ultra micro air-flow crushing, mixed
Close processing step and be prepared into 26% nitre benzene bacterium ester cyflufenamid wettable powder.
Example of formulations 17
Weigh 40% nitre benzene bacterium ester, 1% cyflufenamid, 5% alkylphenol-polyethenoxy base ether phosphate, 4% alkyl naphthalene sulfonic acid
Salt formaldehyde condensation products, 2% sodium alkyl sulfonate, white carbon are filled up to 100% weight portion.Above-mentioned raw materials are blended, ultra micro air-flow powder
Broken, hybrid technique step is prepared into 41% nitre benzene bacterium ester cyflufenamid wettable powder.
Example of formulations 18
Weigh 1% nitre benzene bacterium ester, 50% cyflufenamid, 5% calcium lignosulfonate, 5% alkylnaphthalene sulfonate formaldehyde condensation products,
2% lauryl sodium sulfate, diatomite is filled up to 100% weight portion.Above-mentioned raw materials are blended, ultra micro air-flow crushing, mixing work
Skill step is prepared into 51% nitre benzene bacterium ester cyflufenamid wettable powder.
Example of formulations 19
Weigh 30% nitre benzene bacterium ester, 1% cyflufenamid, 5% polycarboxylate TERSPERSE 2700,4% sodium lignin sulfonate,
1% Nekal BX（Nekal）、1%K-12（Lauryl sodium sulfate）, 1% polyethylene glycol, 2% starch, talcum powder adds
To 100% weight portion.The method of the frequent regulation water intaking dispersible granule of above-mentioned raw materials is mixing, ultra micro air-flow crushing, mixing, granulation
Step produces 31% nitre benzene bacterium ester cyflufenamid water dispersible granules.
Example of formulations 20
Weigh 1% nitre benzene bacterium ester, 40% cyflufenamid, 4% polycarboxylate T36,2% Negel, 2% Nekal BX（Two
Sodium butylnaphthalenesulfonate）, 2% lauryl sodium sulfate, attapulgite adds to 100% weight portion.The frequent regulation water intaking point of above-mentioned raw materials
The method of shot agent is that mixing, ultra micro air-flow crushing, mixing, granulation step are produced 41% nitre benzene bacterium ester cyflufenamid moisture and dissipated
Granula.
Example of formulations 21
Weigh 1% nitre benzene bacterium ester, 30% cyflufenamid, 2% polycarboxylate GY-D06,5% dispersing agent NNO（Alkylnaphthalene sulfonate
Formaldehyde condensation products）, 2% Nekal BX（Nekal）, 3% neopelex, potter's clay adds to 100% weight portion.
The method of above-mentioned raw materials frequent regulation water intaking dispersible granule is that mixing, ultra micro air-flow crushing, mixing, granulation step produce 31% nitre
Benzene bacterium ester cyflufenamid water dispersible granules.
Biological Examples 1：Preventing and treating wheat powdery mildew field test.
Example of formulations 1 has been carried out in Shaanxi Province Mei County within 2015（22% nitre benzene bacterium ester cyflufenamid suspending agent）, system
Agent embodiment 4（16% nitre benzene bacterium ester cyflufenamid microemulsion）, example of formulations 7（37% nitre benzene bacterium ester cyflufenamid water
Emulsion）Preventing and treating wheat powdery mildew field test, demonstrates preventing and treating effect of 3 preparations of different dosage forms of the above to wheat powdery mildew
Really, effective dose and the security to wheat.Test method reference《GB/T 17980.22-2000 pesticide field efficacy medicine tests
Criterion (one) bactericidal agent for preventing and treating Cereal powdery mildew》.Trial crops are wheat, controlling object be powdery mildew (Erysiphe graminis).Carried out in the wheat base of continuous plantation more than 3 years.Test medicine and dosage are to refer to table 4.Separately set blank pair
According to, 4 repetitions often being processed, often the ㎡ of cell 30, totally 24 cells, random district's groups are arranged.Using conventional spray-on process, mu dispenser liquid
50kg, uniformly sprays complete stool.In wheat seedling powdery mildew early stage, the 2nd medication after 7 days is total to dispenser 2 times for 1st dispenser.
Investigation and statistical method：7 days investigation blade incidences after 7 days and the 2nd medicine after 1st medicine.It is diagonal per cell
5 points of samplings of line, every takes 20 plants, upper 3 leaves of every plant of investigation（Having boot leaf then includes boot leaf）.
Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%~15% of whole leaf area；
5 grades：Lesion area accounts for the 16%~25% of whole leaf area；
7 grades：Lesion area accounts for the 26%~50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area.
Calculating preventive effect is referred to according to disease.In situation is influenceed on wheat growth, leaf color etc. after visual observations dispenser after each medicine, examine
Look into security of the medicament to wheat plant.
Disease index=[∑ (the sick numbers of sheets at different levels × relative value of series)/(investigation total leaf number × 9)] × 100
Prevention effect (%)=[(control disease index-treatment disease index)/control disease index] × 100
Table 4 prevents and treats wheat powdery mildew field test results
Table of field test results is bright：22% nitre benzene bacterium ester cyflufenamid suspending agent, 16% nitre benzene bacterium ester cyflufenamid
Microemulsion, 37% nitre benzene bacterium ester cyflufenamid aqueous emulsion have good prevention effect to wheat powdery mildew, respectively by effectively into
Divide 15g/ mus of spraying, be 93.06%, 93.72%, 94.13% to the preventive effects of 7 days after the 1st medicine of wheat powdery mildew, 7 after the 2nd medicine
It preventive effect is 93.16%, 94.20%, 92.49%, hence it is evident that better than 8g/ mus of 10% cyflufenamid suspending agent, 36% nitre benzene bacterium emulsifiable concentrate
10g/ mus of preventive effect.7 days significance difference analysis after to medicine twice, difference reaches the pole level of signifiance（It is shown in Table 4）.
To the safety observations of wheat, 1 day to 7 days visual observations reagent agent is tied to the security of wheat after each medicine
Fruit shows that this several medicament is produced to wheat stalk, blade etc. without poisoning, performance safety.
Except wheat powdery mildew, above-mentioned 3 example of formulations are made when active ingredient dosage is 15g/ mus to Cereal
Thing barley powdery mildew also has synergistic effect, and preventive effect is up to more than 90%.
Biological Examples 2：Prevent and treat powdery mildew of cucumber field test.
Example of formulations 10 has been carried out in Shouguang City of Shandong Province within 2015（21% nitre benzene bacterium ester cyflufenamid aqua）, system
Agent embodiment 13（18% nitre benzene bacterium ester cyflufenamid dispersibles oil-suspending agent）Powdery mildew of cucumber field test is prevented and treated, is demonstrated
Prevention effect of the medicament to powdery mildew of cucumber and the security to cucumber.Trial crops are cucumber, and controlling object is that cucumber is white
Powder disease（Sphaerotheca fuliginea）.Experiment is located at Shouguang City rice field Zhen Dong rice fields village, and experimental plot physical features is flat, soil
It is loam, middle fertility, pH value 6.9, rich water quality management is medium during experiment.Test medicine and dosage refer to table 5.Separately set blank pair
According to, 4 repetitions often being processed, often the ㎡ of cell 30, totally 20 cells, random district's groups are arranged.Using conventional spray-on process, mu dispenser liquid
45kg, is uniformly sprayed at leave dual sides.
Investigation and statistical method：State of an illness radix is investigated before dispenser, 10~14d is reinvestigated once after last time dispenser, altogether
Investigation 2 times.Random per cell to take at 4 points, every whole blades of 2 plants of investigation, every leaf accounts for the percentage of leaf area according to scab
Classification record.Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%~10% of whole leaf area；
5 grades：Lesion area accounts for the 11%~20% of whole leaf area；
7 grades：Lesion area accounts for the 21%~40% of whole leaf area；
9 grades：Lesion area accounts for more than the 40% of whole leaf area.
Disease index=[∑ (the sick numbers of sheets at different levels × relative value of series)/(investigation total leaf number × 9)] × 100
Prevention effect (%)=[1- (disease index after disease index × treatment dispenser before control dispenser)/（Disease after control dispenser
Disease index before feelings index × treatment dispenser] × 100
Table 5 prevents and treats powdery mildew of cucumber field test results
Table of field test results is bright, and 21% nitre benzene bacterium ester cyflufenamid aqua, 18% nitre benzene bacterium ester cyflufenamid can
Dispersion oil-suspending agent has good prevention effect to powdery mildew of cucumber, is sprayed 2 times by active ingredient 15g/ mus respectively, white to cucumber
The preventive effect of 10 days is respectively 92.35%, 93.16% after powder the 2nd medicine of disease, hence it is evident that better than 8g/ mus of 10% cyflufenamid suspending agent, 36%
The preventive effect of 10g/ mus of nitre benzene bacterium emulsifiable concentrate.10 days significance difference analysis after to medicine, difference reaches the pole level of signifiance（It is shown in Table
5）.
Security survey to cucumber, observes for some days after the 1st day and medicine after spray, and each test process are to cucumber without poisoning
Phenomenon occurs.
Except powdery mildew of cucumber, above-mentioned 2 example of formulations active ingredient dosage be 15g/ mus when, to capsicum white powder
Disease, melon powdery mildew, watermelon powdery mildew also have synergistic effect, and preventive effect is up to more than 90%.
Biological Examples 3：Preventing and treating powdery mildew of strawberry field test.
Example of formulations 16 has been carried out in Chongqing City Banan District Mu Dong towns within 2015（26% nitre benzene bacterium ester cyflufenamid can
WP）, example of formulations 19（31% nitre benzene bacterium ester cyflufenamid water dispersible granules）The examination of preventing and treating powdery mildew of strawberry field
Test, demonstrate prevention effect of the medicament to powdery mildew of strawberry and the security to strawberry.Trial crops are strawberry, controlling object
It is powdery mildew of strawberry（Sphaerotheca macularis）.Experiment is located at Banan District Mu Dong towns, and experimental plot physical features is flat, soil
It is yellow soil, fertility is first-class, pH value 6.7, rich water quality management is medium during experiment.Test medicine and dosage refer to table 6.Separately set blank
Compare, often 4 repetitions for the treatment of, every ㎡ of cell 30, totally 20 cells, random district's groups arrangement.Using conventional spray-on process, mu dispenser liquid
45kg, is uniformly sprayed at leave dual sides.
Investigation and statistical method：State of an illness radix is investigated before dispenser, 7~10d is reinvestigated once after last time dispenser, adjusted altogether
Look into 2 times.Per the 5 points of samplings of cell diagonal, every is investigated 3 plants, every plant of whole blades of investigation.Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%~15% of whole leaf area；
5 grades：Lesion area accounts for the 16%~25% of whole leaf area；
7 grades：Lesion area accounts for the 26%~50% of whole leaf area；
9 grades：Lesion area accounts for more than the 51% of whole leaf area；
Disease index=[∑ (the sick numbers of sheets at different levels × relative value of series)/(investigation total leaf number × 9)] × 100
Prevention effect (%)=[1- (disease index after disease index × treatment dispenser before control dispenser)/（Disease after control dispenser
Disease index before feelings index × treatment dispenser] × 100
Table 6 prevents and treats powdery mildew of strawberry field test results
Table of field test results is bright, 26% nitre benzene bacterium ester cyflufenamid wettable powder, 31% nitre benzene bacterium ester ring fluorine
Bacterium aqueous amine dispersible granule has good prevention effect to powdery mildew of strawberry, is sprayed 2 times by active ingredient 15g/ mus respectively, to strawberry
The preventive effect of 10 days is respectively 94.77%, 93.21% after the 2nd medicine of powdery mildew, hence it is evident that better than 8g/ mus of 10% cyflufenamid suspending agent,
The preventive effect of 36% 10g/ mus of nitre benzene bacterium emulsifiable concentrate.10 days significance difference analysis after to medicine, difference reaches the pole level of signifiance（See
Table 6）.
Security survey to strawberry, observes for some days after the 1st day and medicine after spray, and each test process are to strawberry without poisoning
Phenomenon occurs.
Except powdery mildew of strawberry, above-mentioned 2 example of formulations active ingredient dosage be 15g/ mus when, to apple white powder
Disease, uncinula necator also have synergistic effect, and preventive effect is up to more than 90%.
Above example illustrates that the first active component nitre benzene bacterium ester and the second active component cyflufenamid are by weight 1：50
~50：1 compounding, can be made into suspending agent, microemulsion, aqueous emulsion, aqua, dispersibles oil-suspending agent, wettable powder, water-dispersible grain
Agent formulation, there is significant prevention effect to crop powdery mildew, and substantially, dosage is few, prevention effect is high for synergistic effect, and to crop
Security is good.
In sum, composition of the invention is that, using two kinds of active components compound scheme, its activity and bactericidal effect are not
It is the simple superposition of each component activity, compared with existing unitary agent, in addition to significant bactericidal effect, more has significantly
Synergistic effect, to making, security is good, meets the security requirement of pesticidal preparations.
Claims (6)
1. a kind of bactericidal composition containing nitre benzene bacterium ester and cyflufenamid, it is characterised in that：Active ingredient by the first activity into
Nitre benzene bacterium ester and the second active component cyflufenamid is divided to constitute, the weight ratio of the first active component and the second active component is 1：50
~50：1.
2. the bactericidal composition containing nitre benzene bacterium ester and cyflufenamid according to claim 1, it is characterised in that the first activity
The weight ratio of composition and the second active component is 1：1~40：1.
3. the bactericidal composition containing nitre benzene bacterium ester and cyflufenamid according to claim 1 or claim 2, it is characterised in that first
The weight ratio of active component and the second active component is 5：1~36：1.
4. the bactericidal composition containing nitre benzene bacterium ester and cyflufenamid according to claim 1, it is characterised in that the combination
Agent type is suspending agent, microemulsion, aqueous emulsion, aqua, dispersibles oil-suspending agent, wettable powder, water dispersible granules.
5. the bactericidal composition according to claim 1 containing nitre benzene bacterium ester and cyflufenamid is on preventing and treating crop powdery mildew
Purposes.
6. purposes according to claim 5, crop powdery mildew is cereal crop wheat powdery mildew, barley powdery mildew, apple
Fruit tree powdery mildew, vine powdery mildew, powdery mildew of cucumber, melon powdery mildew, watermelon powdery mildew, powdery mildew of strawberry.
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Citations (1)
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|---|---|---|---|---|
| CN101060780A (en) * | 2004-10-14 | 2007-10-24 | 美国陶氏益农公司 | Isomeric mixtures of dinitro-octylphenyl esters and synergistic fungicidal mixtures therefrom |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101060780A (en) * | 2004-10-14 | 2007-10-24 | 美国陶氏益农公司 | Isomeric mixtures of dinitro-octylphenyl esters and synergistic fungicidal mixtures therefrom |
Non-Patent Citations (2)
| Title |
|---|
| Developmental Toxicity of Dinocap in the Mouse Is Not Due to Two Isomers of the Major Active Ingredients;John M. Rogers等;《Teratogenesis, Carcinogenesis, and Mutagenesis》;19871231;第7卷;第341-346页 * |
| 杀菌剂环氟菌胺的开发;程志明;《世界农药》;20071231;第29卷(第6期);第1-4页 * |
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