CN104960006B - A kind of rubber tapping method of gas defence gel cutter - Google Patents
A kind of rubber tapping method of gas defence gel cutter Download PDFInfo
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- CN104960006B CN104960006B CN201510300790.XA CN201510300790A CN104960006B CN 104960006 B CN104960006 B CN 104960006B CN 201510300790 A CN201510300790 A CN 201510300790A CN 104960006 B CN104960006 B CN 104960006B
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- grid
- chassis
- gel
- cover
- cover lid
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Abstract
The present invention relates to a kind of rubber tapping method of gas defence gel cutter, wherein gas defence gel cutter includes chassis and cold cut cutter, described chassis is square, chassis is provided with the first grid, described cold cut cutter covers for cover, the top of described cover lid is provided with the second grid, the first described grid is identical with the second grid, cover is covered provided with cutting needle or cutting sheet, described chassis matches with cover lid, shape and the position of the gel of required cutting are can determine that by the grid on chassis, coordinate cover to cover and rubber tapping is completed in imager, people and ultraviolet when avoiding rubber tapping, ED staining agents, so as to avoid ultraviolet, harm of the ED coloring agents to human body.
Description
Technical field
The present invention relates to a kind of gelatin cutting device, is a kind of automatic gel cutter of gas defence and imager specifically
And its rubber tapping method.
Background technology
Biotechnology technology the most frequently used at present just belongs to round pcr, and research gene molecule will from here on, PCR
The checking of product most basic is exactly that sample is run into agarose gel electrophoresis, is carried out by the size of the gene molecule amount of amplification
Electrophoretic separation, form the band of different molecular weight size gene.Gene band after separation is needed after being imaged by imager
It can see, and gel will be imaged, and must have EB coloring agents and ultraviolet irradiation.EB coloring agents are a kind of super-sensitive glimmering
Light coloring agent, excited with standard 302nm ultraviolet light transmission instrument and radiate orange red chrominance signal, but EB has certain carcinogenicity,
It can volatilize, health is easily poisoned in laboratory.Although nontoxic EB substituting agents, expensive effect have been invented by present foreign countries
There is no that EB is good, therefore EB or main flow.Now with special gel imager, ultraviolet lamp built in the inside, because EB needs purple
Outside line could be imaged, so needing out uviol lamp in imaging, imager present at present is all directly observed by computer to be avoided
Irradiation of the ultraviolet to people.
Although present this technology people is the two murders by poisoning of seldom contact, also have one in the research of some gene molecules
Individual necessary step, be exactly " rubber tapping recovery ", so-called rubber tapping recovery be exactly the gel run through it is relative in requisition for molecular weight
Glue is cut down.Ago-Gel due to running through electrophoresis is still transparent blob of viscose, in ultraviolet photograph only after being dyed through EB
Just it is observed that the gene of the molecular weight required for oneself is in which particular location, so current rubber tapping is all under conditions of penetrating
The manual rubber tapping that artificial exposure is carried out under ultraviolet light, EB are also easy to diffusion and are detrimental to health in atmosphere.
Therefore, it is necessary to which a kind of be avoided that ultraviolet and EB harmful to human, the device of safety and precise rubber tapping.On such dress
Put, yet there are no report.
The content of the invention
The purpose of the present invention is to be directed to deficiency of the prior art, there is provided a kind of gas defence gel cutter.
The another purpose of the present invention is to provide a kind of imager provided with gel cutter.
The another purpose of the present invention is to provide a kind of rubber tapping method.
To achieve the above object, the present invention adopts the technical scheme that：
A kind of gas defence gel cutter, including chassis and cold cut cutter, described chassis are provided with the first grid.
Described cold cut cutter covers for cover, and the top of described cover lid is provided with the second grid, the first described grid and the
Two grids are identical, and described cover covers and through hole is provided with the node of the second grid, are slid in through hole and are connected with cutting needle, described
Cutting needle entirety rectangular array shape, described cover lid match with chassis.
Described cold cut cutter covers for cover, and the top of described cover lid is provided with the second grid, the first described grid and the
Two grids are identical, and the inside top of described cover lid is provided with articulamentum, and articulamentum is provided with cutting sheet, and described cutting sheet is band
Shape, it is made up of flexible metallic material, described cover lid matches with chassis.
Described cold cut cutter is automatic gas cutting machine, and described automatic gas cutting machine is provided with base, the first travel mechanism, second
Travel mechanism, controller and cutter head, described base are provided with guide rail, and the first described travel mechanism is slided by guide rail and base
Dynamic connection, the second described travel mechanism and the first travel mechanism are slidably connected, and the output end of described controller is moved with first
Motivation structure, the connection of the second travel mechanism, the input of controller are connected with computer, and described cutter head is fixed on the second moving machine
On structure, described chassis is located on base.
To realize above-mentioned second purpose, the present invention adopts the technical scheme that：
A kind of imager, described imager is interior to be provided with gas defence gel cutter.
Described gas defence gel cutter is that chassis and cover cover, and the inner upper of described imager is provided with slideway, slideway
Slide device is provided with, cylinder is connected with below described slide device, cylinder lower section and the cover lid of gas defence gel cutter connect
Connect, the wall box of imager is provided with port, and described passage port is provided with sealed door.
Described gas defence gel cutter is chassis and automatic gas cutting machine.
To realize above-mentioned 3rd purpose, the present invention adopts the technical scheme that：
A kind of rubber tapping method, described rubber tapping method are that gel is placed on chassis, are then placed in imager and are imaged,
One picture for including gene beam shapes and the first grid is shot by computer, picture is compareed, cover is covered at corresponding node
Cutting needle head into cover lid or cutting sheet bent to the shape recorded on picture and is fixed on corresponding position on articulamentum,
Finally cover cover buckle can be completed to tap rubber on chassis.
A kind of rubber tapping method, described rubber tapping method are that gel is placed on chassis, are then imaged in imager, are led to
Cross computer and shoot a picture for including gene beam shapes and the first grid, according to the position of required cutting gel and shape
Shape, cover is covered the cutting needle at corresponding node and head into cover lid or bend to cutting sheet the shape recorded on picture and fixation
Cover lid, is then fixed on the cylinder below slide device by the corresponding position on articulamentum, is slided to cover lid by slideway
Directly over chassis, pushed in cylinder driving lower cover lid, cover lid is covered on chassis, completes cutting.
A kind of rubber tapping method, described rubber tapping method are that automatic gas cutting machine is provided with imager, and gel is in imager
After imaging, information transmission to controller, the controller for the shape that needs are cut by computer passes through the first travel mechanism,
Two travel mechanisms control cutter head is cut.
The invention has the advantages that：
The present invention can determine that shape and the position of the gel of required cutting by the grid on chassis, coordinates cover to cover and is being imaged
Rubber tapping is completed in instrument, people contacts with ultraviolet, ED when avoiding rubber tapping, so as to avoid the harm of ultraviolet, ED to human body.
Brief description of the drawings
Accompanying drawing 1 is the structural representation on chassis.
Accompanying drawing 2 is the structural representation for cutting needle housing lid.
Accompanying drawing 3 is the structural representation of cutting sheet cover lid.
Accompanying drawing 4 is the structural representation of imager.
Accompanying drawing 5 is the structural representation of automatic gas cutting machine.
Embodiment
Embodiment provided by the invention is elaborated below in conjunction with the accompanying drawings.
The reference and part being related in accompanying drawing are as follows：
1. the grid of chassis 11. first
2. the grid of cover lid 21. second
22. the cutting needle of through hole 23.
24. the imager of cutting sheet 3.
31. the slideway of sealed door 4.
41. the cylinder of slide device 42.
51. the guide rail of base 52.
53. travel mechanism of the first travel mechanism 54. second
55. the blade of controller 56..
Embodiment 1
Fig. 1 is refer to, Fig. 1 is the structural representation on chassis.To be square, described chassis 1 is provided with the on described chassis 1
One grid 11.
Gel after dyeing is placed on chassis 1 and is together put into imager, is carried out after opening ultraviolet lamp by computer
Observation, while the photo containing the first grid 11 in gene beam shapes and chassis 1 is shot, picture is compareed, artificially using cold
Instrument is cut on the chassis 1 for having grid 11.
Embodiment 2
Fig. 2 is refer to, Fig. 2 is the structural representation for cutting needle housing lid.Described cover lid 2 is provided with the second grid 21, the
Through hole 22 is provided with the node of two grids 21, is slid in through hole 22 and is connected with cutting needle 23, overall described cutting needle 23 is in square
Shape array-like.
Gel is placed on chassis 1, is then placed in imager and is imaged, shooting one by computer includes gene strip-form
The picture of shape and the first grid 11, picture is compareed, the cutting needle 23 on cover lid 2 at corresponding node is headed into cover lid 2, finally will
Cover lid 2, which is buckled on chassis 1, can complete to tap rubber.
Embodiment 3
It refer to Fig. 3, the structural representation of Fig. 3 cutting sheet cover lids.Described cover lid 2 is provided with the second grid 21, cover lid 2
Inside top be provided with articulamentum (not shown), articulamentum is provided with cutting sheet 24, and described cutting sheet 24 is banding, by flexibility
Metal material is made.
Gel is placed on chassis 1, is then placed in imager and is imaged, shooting one by computer includes gene strip-form
The picture of shape and the first grid 11, picture is compareed, cutting sheet 24 is bent to the shape recorded on picture, and be fixed on articulamentum
Upper corresponding position, finally cover lid 2, which is buckled on chassis 1, can complete to tap rubber.
Embodiment 4
Fig. 4 is refer to, Fig. 4 is the structural representation of imager.The wall box of described imager 3 is provided with port
(not shown), passage port are provided with sealed door 31, and the inner upper of described imager 3 is provided with slideway 4, and slideway is provided with sliding and filled
41 are put, described slide device 41 is provided with cylinder 42, and one end of described cylinder 42 is connected with cover lid 2, and described cover lid 2 is
Cut needle housing lid or cutting sheet cover lid.
After gel is imaged in imager 3, shooting one by computer includes gene beam shapes and the first grid 11
Picture, according to it is required cutting gel position and shape, the cutting needle 23 on cover lid 2 at corresponding node is headed into cover lid 2
It is interior or cutting sheet 24 is bent into the shape recorded on picture and is fixed on corresponding position on articulamentum, then cover lid 2 is consolidated
It is scheduled on the cylinder 42 of the lower section of slide device 41, afterwards together slides into cover lid 2 and cylinder 42 in imager, and by closed
Door 31 closes port, prevents coloring agent to be diffused into outside imager 3, and when cover lid 2 is slided to directly over chassis 1, cylinder 42 drives cover lid 2
Push, cover lid 2 is covered on chassis 1, completes cutting.
Embodiment 5
Fig. 5 is refer to, Fig. 5 is the structural representation of automatic gas cutting machine.Described automatic gas cutting machine is provided with base 51, first
Travel mechanism 53, the second travel mechanism 54, controller 55 and cutter head 56, described base 51 are provided with guide rail 52, and described
One travel mechanism 53 is slidably connected by guide rail 52 and base 51, and the second described travel mechanism 54 and the first travel mechanism 53 are slided
Dynamic connection, the output end of described controller 55 are connected with the first travel mechanism 53, the second travel mechanism 54, controller 55 it is defeated
Enter end to be connected with computer, described cutter head 56 is fixed in the second travel mechanism 54.
Gel after dyeing is placed on chassis 1 and is then placed in imager, is carried out after opening ultraviolet lamp by computer
Observation, while the photo containing the first grid 11 in gene beam shapes and chassis 1 is shot, surface trimming is installed in imager
Machine, information transmission to controller, the controller 55 for the shape that needs are cut by computer can control the first moving machine respectively
Structure 53, the second travel mechanism 54 are mobile around, so as to control cutter head 56 to be cut.
Embodiment 6
Gel after dyeing is placed on chassis 1 and is together put into imager, is carried out after opening ultraviolet lamp by computer
Observation, while the photo containing the first grid 11 in gene beam shapes and chassis 1 is shot, picture is compareed, it is determined that needing to cut
Cut the shape of gel；Then gel and chassis 1 are transferred on the base 51 of automatic gas cutting machine；The letter for the shape cut will be needed
Breath is input in computer, controls automatic gas cutting machine to carry out cutting gel by computer.
Embodiment 1-3 and embodiment 6 can accurately determine the position of gene band when cutting gel by the first grid 11,
No longer need to cut under ultraviolet light afterwards, avoid irradiation of the ultraviolet to human body；Now the effect of coloring agent has reached,
Cut again after can first coloring agent will be removed, avoid the harm of noxious material.
Embodiment 4-5 rubber tapping process is completed in imager 3, is avoided ultraviolet and is radiated on human body, is imaged simultaneously
Instrument 3 is always maintained at closed, prevents EB coloring agents from diffusing to harmful to human in air.
It should be noted that the first described grid 11 and the second grid 22 use identical specification, mesh-density is bigger,
The precision of cutting is higher, and in the case where the node of grid is enough, the colloid of needs can be directly cut out by cutting needle,
And in the case where grid node is less, cutting needle 23 can be first passed through and primarily determine that cutting profile, then artificially cut by instrument
Cut, now cutting needle 23 act as positioning；The wall box of described imager is provided with port, is easy to cover lid 2 and cylinder
42 are slided into imager, and described port is sealed by sealed door 31 under normal conditions, and its role is to prevent dust etc.
Enter in imager, while avoid the coloring agent in rubber tapping from being diffused into outside imager, sealing structure can also use flexible
Self-enclosing.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, on the premise of the inventive method is not departed from, can also make some improvement and supplement, and these are improved and supplement also should be regarded as
Protection scope of the present invention.
Claims (4)
1. a kind of rubber tapping method of gas defence gel cutter, gas defence gel cutter includes chassis and cold cut cutter, described bottom
Disk is provided with the first grid, and described cold cut cutter covers for cover, and the top of described cover lid is provided with the second grid, and described first
Grid is identical with the second grid, and described cover covers and through hole is provided with the node of the second grid, is slid in through hole and is connected with cutting
Pin, described cutting needle entirety rectangular array shape, described cover lid match with chassis；Characterized in that, gel is placed on
On chassis, it is then placed in imager and is imaged, a picture for including gene beam shapes and the first grid is shot by computer,
Picture is compareed, cover is covered the cutting needle at corresponding node and headed into cover lid, finally can complete to cut on chassis by cover cover buckle
Glue.
2. a kind of rubber tapping method of gas defence gel cutter, gas defence gel cutter includes chassis and cold cut cutter, described bottom
Disk is provided with the first grid, and described cold cut cutter covers for cover, and the top of described cover lid is provided with the second grid, and described first
Grid is identical with the second grid, and the inside top of described cover lid is provided with articulamentum, and articulamentum is provided with cutting sheet, and described cuts
Pitch cutting is banding, is made up of flexible metallic material, and described cover lid matches with chassis, it is characterised in that gel is placed on into bottom
On disk, it is then placed in imager and is imaged, a picture for including gene beam shapes and the first grid is shot by computer, it is right
Photograph and picture, cutting sheet is bent to the shape recorded on picture and is fixed on corresponding position on articulamentum, finally by cover cover buckle
It can complete to tap rubber on chassis.
3. a kind of rubber tapping method of gas defence gel cutter, imager is interior to be provided with gas defence gel cutter, gas defence gel cutter
Including chassis and cold cut cutter, described chassis is provided with the first grid, and described cold cut cutter covers for cover, described cover lid
Top is provided with the second grid, and the first described grid is identical with the second grid, and described cover covers to be set at the node of the second grid
There is through hole, slid in through hole and be connected with cutting needle, described cutting needle entirety rectangular array shape, described cover lid and chassis phase
Matching；The inner upper of described imager is provided with slideway, and slideway is provided with slide device, connected below described slide device
There is cylinder, be connected below cylinder with the cover lid of gas defence gel cutter, the wall box of imager is provided with port, and described is logical
Sealed door is provided with mouthful, it is characterised in that gel is placed on chassis, is then imaged in imager, one is shot by computer
Include the picture of gene beam shapes and the first grid, according to the required position for cutting gel and shape, cover is covered pair
Answer the cutting needle at node to head into cover lid, then cover lid is fixed on the cylinder below slide device, will be covered by slideway
Lid is slided to directly over chassis, is pushed in cylinder driving lower cover lid, cover lid is covered on chassis, completes cutting.
4. a kind of rubber tapping method of gas defence gel cutter, imager is interior to be provided with gas defence gel cutter, gas defence gel cutter
Including chassis and cold cut cutter, described chassis is provided with the first grid, and described cold cut cutter covers for cover, described cover lid
Top is provided with the second grid, and the first described grid is identical with the second grid, and the inside top of described cover lid is provided with connection
Layer, articulamentum are provided with cutting sheet, and described cutting sheet is banding, is made up of flexible metallic material, described cover lid and chassis
Match, the inner upper of described imager is provided with slideway, and slideway is provided with slide device, connected below described slide device
Cylinder is connected to, is connected below cylinder with the cover lid of gas defence gel cutter, the wall box of imager is provided with port, described
Passage port is provided with sealed door, it is characterised in that gel is placed on chassis, is then imaged in imager, is shot by computer
One picture for including gene beam shapes and the first grid, according to the position of required cutting gel and shape, by cutting sheet
Bend to the shape recorded on picture and be fixed on corresponding position on articulamentum, then cover lid is fixed on below slide device
Cylinder on, cover lid is slided to directly over chassis by slideway, pushed in cylinder driving lower cover lid, cover lid is covered on chassis, complete
Into cutting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510300790.XA CN104960006B (en) | 2015-06-04 | 2015-06-04 | A kind of rubber tapping method of gas defence gel cutter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510300790.XA CN104960006B (en) | 2015-06-04 | 2015-06-04 | A kind of rubber tapping method of gas defence gel cutter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104960006A CN104960006A (en) | 2015-10-07 |
| CN104960006B true CN104960006B (en) | 2018-01-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510300790.XA Expired - Fee Related CN104960006B (en) | 2015-06-04 | 2015-06-04 | A kind of rubber tapping method of gas defence gel cutter |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110161171B (en) * | 2018-02-14 | 2022-02-18 | 广东医科大学 | Method for high-throughput screening of bioactive components in complex components by using planar chromatography component microarray and application of method |
| CN110161170B (en) * | 2018-02-14 | 2022-02-22 | 广东医科大学 | Sample processing device and biological activity screening system containing same |
| CN108527462A (en) * | 2018-04-28 | 2018-09-14 | 福建农林大学 | A kind of high-cleanness, high based on L formula running gel rubber knifes cuts gluing method |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6691697B2 (en) * | 2002-05-03 | 2004-02-17 | Hon Hai Precision Ind. Co., Ltd. | Method for cutting thin film filter work pieces |
| CN2562950Y (en) * | 2002-09-03 | 2003-07-30 | 张宜静 | hand operate multipurpose punching device |
| CN201864730U (en) * | 2010-11-02 | 2011-06-15 | 浙江理工大学 | Portable ultraviolet gel cutter |
| CN103196909B (en) * | 2012-01-10 | 2015-08-12 | 卢志强 | A kind of gel image analyser |
| CN203109992U (en) * | 2013-03-11 | 2013-08-07 | 江苏匡亚生物医药科技有限公司 | Automatic deoxyribonucleic acid (DNA) gel cutting device |
| CN203405440U (en) * | 2013-08-05 | 2014-01-22 | 广州杰特伟生物科技有限公司 | Gel imaging and cutting instrument |
| CN103522324A (en) * | 2013-10-21 | 2014-01-22 | 西北农林科技大学 | Gel special cutter |
| CN204037006U (en) * | 2014-09-04 | 2014-12-24 | 昆山超雄精密模具有限公司 | Silicone rubber products pastes back bakelized paper fast and removes the mould of burr |
| CN204325332U (en) * | 2014-11-11 | 2015-05-13 | 张军峰 | A kind of gel device for cutting automatically |
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| CN104960006A (en) | 2015-10-07 |
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Granted publication date: 20180116 |