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CA3223669A1 - Sequencage simultane de brins complementaires sens et antisens sur des polynucleotides concatenes pour la detection de methylation - Google Patents

Sequencage simultane de brins complementaires sens et antisens sur des polynucleotides concatenes pour la detection de methylation Download PDF

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Publication number
CA3223669A1
CA3223669A1 CA3223669A CA3223669A CA3223669A1 CA 3223669 A1 CA3223669 A1 CA 3223669A1 CA 3223669 A CA3223669 A CA 3223669A CA 3223669 A CA3223669 A CA 3223669A CA 3223669 A1 CA3223669 A1 CA 3223669A1
Authority
CA
Canada
Prior art keywords
sequence
sequencing
complement
polynucleotide
signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3223669A
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English (en)
Inventor
Niall Gormley
Jonathan Boutell
Aathavan KARUNAKARAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of CA3223669A1 publication Critical patent/CA3223669A1/fr
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés de détection de cytosines modifiées dans des séquences d'acides nucléiques.
CA3223669A 2022-03-15 2023-03-15 Sequencage simultane de brins complementaires sens et antisens sur des polynucleotides concatenes pour la detection de methylation Pending CA3223669A1 (fr)

Applications Claiming Priority (23)

Application Number Priority Date Filing Date Title
US202263269383P 2022-03-15 2022-03-15
US63/269,383 2022-03-15
US202263328444P 2022-04-07 2022-04-07
US63/328,444 2022-04-07
US202363439443P 2023-01-17 2023-01-17
US202363439438P 2023-01-17 2023-01-17
US202363439491P 2023-01-17 2023-01-17
US202363439522P 2023-01-17 2023-01-17
US202363439466P 2023-01-17 2023-01-17
US202363439417P 2023-01-17 2023-01-17
US202363439519P 2023-01-17 2023-01-17
US202363439415P 2023-01-17 2023-01-17
US202363439501P 2023-01-17 2023-01-17
US63/439,466 2023-01-17
US63/439,417 2023-01-17
US63/439,501 2023-01-17
US63/439,438 2023-01-17
US63/439,491 2023-01-17
US63/439,522 2023-01-17
US63/439,415 2023-01-17
US63/439,443 2023-01-17
US63/439,519 2023-01-17
PCT/EP2023/056668 WO2023175040A2 (fr) 2022-03-15 2023-03-15 Séquençage simultané de brins complémentaires sens et antisens sur des polynucléotides concaténés pour la détection de méthylation

Publications (1)

Publication Number Publication Date
CA3223669A1 true CA3223669A1 (fr) 2023-09-21

Family

ID=85800563

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3223669A Pending CA3223669A1 (fr) 2022-03-15 2023-03-15 Sequencage simultane de brins complementaires sens et antisens sur des polynucleotides concatenes pour la detection de methylation
CA3223653A Pending CA3223653A1 (fr) 2022-03-15 2023-03-15 Sequencage simultane de brins de complement avant et inverse sur des polynucleotides separes pour la detection de methylation

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3223653A Pending CA3223653A1 (fr) 2022-03-15 2023-03-15 Sequencage simultane de brins de complement avant et inverse sur des polynucleotides separes pour la detection de methylation

Country Status (4)

Country Link
EP (2) EP4341426A2 (fr)
AU (2) AU2023233839A1 (fr)
CA (2) CA3223669A1 (fr)
WO (2) WO2023175037A2 (fr)

Family Cites Families (34)

* Cited by examiner, † Cited by third party
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EP0972081B1 (fr) 1997-04-01 2007-06-13 Solexa Ltd. Methode d'amplification d'acide nucleique
ATE269908T1 (de) 1997-04-01 2004-07-15 Manteia S A Methode zur sequenzierung von nukleinsäuren
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
AU2001251582A1 (en) 2000-04-14 2001-10-30 Lynx Therapeutics, Inc. Method and compositions for ordering restriction fragments
NZ523831A (en) 2000-07-13 2005-10-28 Invitrogen Corp Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
CA2478722A1 (fr) 2002-03-05 2003-09-12 Solexa Limited Procedes de detection de variations de sequence a l'echelle du genome associees a un phenotype
CA2727850C (fr) * 2003-01-29 2013-04-30 454 Corporation Procede d'amplification et de sequencage d'acides nucleiques
WO2005039389A2 (fr) * 2003-10-22 2005-05-06 454 Corporation Caryotypage base sur des séquences
EP1682680B2 (fr) 2003-10-31 2018-03-21 AB Advanced Genetic Analysis Corporation Procedes de production d'une etiquette appariee a partir d'une sequence d'acides nucleiques et methodes d'utilisation associees
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
EP2230316A1 (fr) 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Sequencage nucléotidique comprenant des cycles sucessifs de fromation duplex
CA2604095A1 (fr) 2005-04-12 2006-10-19 454 Life Sciences Corporation Procedes de determination de variantes de sequence utilisant un sequencage des amplicons
WO2007145612A1 (fr) 2005-06-06 2007-12-21 454 Life Sciences Corporation Séquençage d'extrémités appariées
US8428882B2 (en) 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
GB0514910D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
SG169356A1 (en) 2006-02-08 2011-03-30 Illumina Cambridge Ltd Method for sequencing a polynucleotide template
EP2021503A1 (fr) 2006-03-17 2009-02-11 Solexa Ltd. Procédés isothermiques pour créer des réseaux moléculaires clonales simples
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008093098A2 (fr) 2007-02-02 2008-08-07 Illumina Cambridge Limited Procedes pour indexer des echantillons et sequencer de multiples matrices nucleotidiques
WO2012170936A2 (fr) 2011-06-09 2012-12-13 Illumina, Inc. Cellules à flux modelé utiles pour l'analyse d'acide nucléique
DK2737085T3 (en) * 2011-07-29 2017-01-23 Cambridge Epigenetix Ltd METHODS FOR DETECTING NUCLEOTID MODIFICATION
PT3290528T (pt) 2011-09-23 2019-10-14 Illumina Inc Métodos e composições para sequenciamento de ácido nucleico
EP3124605A1 (fr) 2012-03-15 2017-02-01 New England Biolabs, Inc. Procedes et compositions pour la discrimination entre la cytosine et leurs modifications et pour l'analyse des methylomes
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
CN106103743A (zh) * 2014-01-07 2016-11-09 Imppc私人基金会 用于产生双链dna文库的方法和用于鉴定甲基化胞嘧啶的测序方法
GB201419731D0 (en) * 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
WO2017075436A1 (fr) 2015-10-30 2017-05-04 New England Biolabs, Inc. Compositions et procédés de détermination de cytosines modifiées par séquençage
WO2019136413A1 (fr) * 2018-01-08 2019-07-11 Ludwig Institute For Cancer Research Ltd Identification par résolution de base sans bisulfite de modifications de cytosine
WO2019136376A1 (fr) 2018-01-08 2019-07-11 Illumina, Inc. Séquençage à haut débit à détection à semi-conducteur
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JP2023547366A (ja) 2020-10-21 2023-11-10 イルミナ インコーポレイテッド 複数のインサートを含む配列決定鋳型、並びに配列決定スループットを改善するための組成物及び方法

Also Published As

Publication number Publication date
CA3223653A1 (fr) 2023-09-21
AU2023234670A1 (en) 2024-01-18
AU2023233839A1 (en) 2024-01-18
WO2023175037A2 (fr) 2023-09-21
EP4341426A2 (fr) 2024-03-27
WO2023175040A3 (fr) 2023-11-02
WO2023175040A2 (fr) 2023-09-21
EP4341425A2 (fr) 2024-03-27
WO2023175037A3 (fr) 2023-11-23

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