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BR112021019657A2 - Método para produzir célula knock-in - Google Patents

Método para produzir célula knock-in

Info

Publication number
BR112021019657A2
BR112021019657A2 BR112021019657A BR112021019657A BR112021019657A2 BR 112021019657 A2 BR112021019657 A2 BR 112021019657A2 BR 112021019657 A BR112021019657 A BR 112021019657A BR 112021019657 A BR112021019657 A BR 112021019657A BR 112021019657 A2 BR112021019657 A2 BR 112021019657A2
Authority
BR
Brazil
Prior art keywords
molecule
genomic
cleaves
homology arm
cell
Prior art date
Application number
BR112021019657A
Other languages
English (en)
Inventor
Kazuto Yoshimi
Makoto Sato
Tomoji Mashimo
Yoshihiro Uno
Yoshiki Miyasaka
Yuichiro Oka
Yuko Kotani
Original Assignee
Univ Osaka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Osaka filed Critical Univ Osaka
Publication of BR112021019657A2 publication Critical patent/BR112021019657A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

a presente invenção refere-se ao uso de um sistema de nuclease específico de sítio que inclui uma combinação de uma molécula que simultaneamente tem como alvo e cliva uma sequência do braço de homologia do dna doador e a sequência genômica correspondente à sequência do braço de homologia e uma molécula que tem como alvo e cliva a região genômica na vizinhança do sítio de clivagem por aquela molécula que causa o reparo entre o dna genômico e o dna doador por meio de junção de extremidade não homóloga e recombinação homóloga, tornando possível a produção de células e organismos com sequências doadoras longas knocked-in com alta eficiência e precisão.
BR112021019657A 2019-04-05 2020-04-03 Método para produzir célula knock-in BR112021019657A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2019072782 2019-04-05
PCT/JP2020/015291 WO2020204159A1 (ja) 2019-04-05 2020-04-03 ノックイン細胞の作製方法

Publications (1)

Publication Number Publication Date
BR112021019657A2 true BR112021019657A2 (pt) 2021-11-30

Family

ID=72668293

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021019657A BR112021019657A2 (pt) 2019-04-05 2020-04-03 Método para produzir célula knock-in

Country Status (9)

Country Link
US (1) US20220186263A1 (pt)
EP (1) EP3950942A4 (pt)
JP (1) JP7426120B2 (pt)
KR (1) KR20210148286A (pt)
CN (1) CN113646429A (pt)
AU (1) AU2020254078A1 (pt)
BR (1) BR112021019657A2 (pt)
CA (1) CA3136061A1 (pt)
WO (1) WO2020204159A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112746074B (zh) * 2020-12-16 2022-12-02 南方医科大学皮肤病医院（广东省皮肤病医院、广东省皮肤性病防治中心、中国麻风防治研究中心） Keratin 5-IRES-eGFP敲入的hESCs细胞系的构建及诱导分化方法
WO2024102954A1 (en) 2022-11-10 2024-05-16 Massachusetts Institute Of Technology Activation induced clipping system (aics)

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100575485C (zh) 2002-01-23 2009-12-30 犹他大学研究基金会 使用锌指核酸酶的定向染色体诱变
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
US8586363B2 (en) 2009-12-10 2013-11-19 Regents Of The University Of Minnesota TAL effector-mediated DNA modification
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
US20140242664A1 (en) 2012-12-12 2014-08-28 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions for sequence manipulation
CN116425883A (zh) 2013-04-22 2023-07-14 国立大学法人九州大学 利用ppr基序的dna结合性蛋白质及其应用
JP5931022B2 (ja) 2013-08-09 2016-06-08 国立大学法人広島大学 Ｄｎａ結合ドメインを含むポリペプチド
US10787684B2 (en) * 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
JP6772067B2 (ja) * 2014-11-20 2020-10-21 国立大学法人京都大学 哺乳動物の標的ゲノム領域にｄｎａをノックインする方法及び細胞
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US11905521B2 (en) 2015-11-17 2024-02-20 The Chinese University Of Hong Kong Methods and systems for targeted gene manipulation
US20200029538A1 (en) * 2016-11-28 2020-01-30 Osaka University Genome editing method
CN110914423B (zh) 2017-05-31 2024-02-06 国立大学法人东京大学 经修饰的Cas9蛋白及其用途
CN117778466A (zh) 2017-06-08 2024-03-29 国立大学法人大阪大学 Dna经编辑的真核细胞制造方法和用于该方法的试剂盒
US12012596B2 (en) 2017-08-21 2024-06-18 Tokushima University Target sequence specific alteration technology using nucleotide target recognition

Also Published As

Publication number Publication date
WO2020204159A1 (ja) 2020-10-08
US20220186263A1 (en) 2022-06-16
EP3950942A1 (en) 2022-02-09
CA3136061A1 (en) 2020-10-08
EP3950942A4 (en) 2023-01-18
KR20210148286A (ko) 2021-12-07
CN113646429A (zh) 2021-11-12
JPWO2020204159A1 (pt) 2020-10-08
AU2020254078A1 (en) 2021-12-02
JP7426120B2 (ja) 2024-02-01

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