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BR112021019368A2 - Método para produzir fcrn biotinilado no terminal c - Google Patents

Método para produzir fcrn biotinilado no terminal c

Info

Publication number
BR112021019368A2
BR112021019368A2 BR112021019368A BR112021019368A BR112021019368A2 BR 112021019368 A2 BR112021019368 A2 BR 112021019368A2 BR 112021019368 A BR112021019368 A BR 112021019368A BR 112021019368 A BR112021019368 A BR 112021019368A BR 112021019368 A2 BR112021019368 A2 BR 112021019368A2
Authority
BR
Brazil
Prior art keywords
fcrn
expression cassette
producing
cassette encoding
encoding
Prior art date
Application number
BR112021019368A
Other languages
English (en)
Inventor
Maria Wehrstein Jasmin
Stefan Seeber
Tilman Schlothauer
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR112021019368A2 publication Critical patent/BR112021019368A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04015—Biotin-[acetyl-CoA-carboxylase] ligase (6.3.4.15)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

método para produzir fcrn biotinilado no terminal c. neste documento é relatado um método para produzir fcrn biotinilado no terminal c compreendendo as etapas de cultivo de uma célula de mamífero compreendendo um ácido desoxirribonucleico que codifica fcrn e e.coli biotina-[acetil-coa-carboxilase] ligase (bira) em um meio contendo biotina, e recuperação de fcrn biotinilado no terminal c a partir da célula ou meio de cultivo, em que o ácido desoxirribonucleico que codifica fcrn e e.coli bira está integrado de forma estável no genoma da célula de mamífero e compreende na direção 5' para 3' um primeiro cassete de expressão que codifica a proteína semelhante ao complexo principal de histocompatibilidade de classe i (a-fcrn) compreendendo um marcador hisavi no terminal c, um segundo cassete de expressão que codifica ß2-microglobulina (ß2m), um terceiro cassete de expressão que codifica a proteína semelhante ao complexo principal de histocompatibilidade de classe i (a-fcrn) compreendendo um marcador hisavi no terminal c, um quarto cassete de expressão que codifica ß2-microglobulina (ß2m) e um quinto cassete de expressão que codifica e.coli biotina-[acetil-coa-carboxilase] ligase.
BR112021019368A 2019-03-29 2020-03-26 Método para produzir fcrn biotinilado no terminal c BR112021019368A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19166030 2019-03-29
PCT/EP2020/058452 WO2020200983A1 (en) 2019-03-29 2020-03-26 Method for the generation of an fcrn expressing cell by targeted integration of multiple expression cassettes in a defined organization

Publications (1)

Publication Number Publication Date
BR112021019368A2 true BR112021019368A2 (pt) 2021-12-07

Family

ID=66239740

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021019368A BR112021019368A2 (pt) 2019-03-29 2020-03-26 Método para produzir fcrn biotinilado no terminal c

Country Status (12)

Country Link
US (1) US20220119482A1 (pt)
EP (1) EP3947437A1 (pt)
JP (1) JP7250950B2 (pt)
KR (1) KR20210134932A (pt)
CN (1) CN113661173B (pt)
AU (1) AU2020253023B2 (pt)
BR (1) BR112021019368A2 (pt)
CA (1) CA3130546A1 (pt)
IL (1) IL286718A (pt)
MX (1) MX2021011837A (pt)
SG (1) SG11202109391PA (pt)
WO (1) WO2020200983A1 (pt)

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2096222C (en) 1990-11-13 1998-12-29 Stephen D. Lupton Bifunctional selectable fusion genes
EP0804590A1 (en) 1993-05-21 1997-11-05 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
AU2005263331B8 (en) 2004-07-20 2011-06-16 Symphogen A/S Anti-Rhesus D recombinant polyclonal antibody and methods of manufacture
WO2007124452A2 (en) 2006-04-20 2007-11-01 The Regents Of The University Of California Methods for expressing multiple sirna and shrna from a single vector
US8293533B2 (en) * 2008-12-19 2012-10-23 E.I. Du Pont De Nemours And Company Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
WO2013006142A1 (en) 2011-07-05 2013-01-10 Nanyang Technological University A novel process and reagent for rapid genetic alterations in eukaryotic cells
US20150018241A1 (en) 2012-02-15 2015-01-15 Hoffmann-La Roche Inc. Fc-receptor based affinity chromatography
US20160046694A1 (en) 2013-03-30 2016-02-18 Usha Biotech Limited Methods and constructs for expressing biologically active proteins in mammalian cells
HK1218486A2 (zh) 2015-12-09 2017-02-17 Ka Lun Alan Ng 提高魚生長的手段和方法
AR108295A1 (es) * 2016-04-20 2018-08-08 Regeneron Pharma Composiciones y métodos para preparar anticuerpos basados en el uso de un locus mejorador de expresión
CA3052357A1 (en) 2017-03-10 2018-09-13 F. Hoffmann-La Roche Ag Method for producing multispecific antibodies
CN110573626B (zh) * 2017-04-28 2024-05-03 豪夫迈·罗氏有限公司 抗体选择方法
KR20240132119A (ko) 2017-12-22 2024-09-02 제넨테크, 인크. 핵산의 표적화된 통합

Also Published As

Publication number Publication date
SG11202109391PA (en) 2021-09-29
JP7250950B2 (ja) 2023-04-03
JP2022526556A (ja) 2022-05-25
US20220119482A1 (en) 2022-04-21
CN113661173A (zh) 2021-11-16
KR20210134932A (ko) 2021-11-11
IL286718A (en) 2021-10-31
CA3130546A1 (en) 2020-10-08
CN113661173B (zh) 2024-10-01
MX2021011837A (es) 2021-10-22
AU2020253023A1 (en) 2021-09-23
EP3947437A1 (en) 2022-02-09
AU2020253023B2 (en) 2022-07-14
WO2020200983A1 (en) 2020-10-08

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