MX2021011837A - Método para la generación de una célula que expresa el receptor neonatal de región de fragmento cristalizable (fcrn) mediante la integración dirigida de múltiples casetes de expresión en una organización definida. - Google Patents
Método para la generación de una célula que expresa el receptor neonatal de región de fragmento cristalizable (fcrn) mediante la integración dirigida de múltiples casetes de expresión en una organización definida.Info
- Publication number
- MX2021011837A MX2021011837A MX2021011837A MX2021011837A MX2021011837A MX 2021011837 A MX2021011837 A MX 2021011837A MX 2021011837 A MX2021011837 A MX 2021011837A MX 2021011837 A MX2021011837 A MX 2021011837A MX 2021011837 A MX2021011837 A MX 2021011837A
- Authority
- MX
- Mexico
- Prior art keywords
- fcrn
- expression cassette
- cassette encoding
- generation
- expression cassettes
- Prior art date
Links
- 101150050927 Fcgrt gene Proteins 0.000 title 1
- 230000010354 integration Effects 0.000 title 1
- 230000008520 organization Effects 0.000 title 1
- 108050003866 Bifunctional ligase/repressor BirA Proteins 0.000 abstract 4
- 102100033743 Biotin-[acetyl-CoA-carboxylase] ligase Human genes 0.000 abstract 4
- 102100026120 IgG receptor FcRn large subunit p51 Human genes 0.000 abstract 4
- 101710177940 IgG receptor FcRn large subunit p51 Proteins 0.000 abstract 4
- 241000588724 Escherichia coli Species 0.000 abstract 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 210000004962 mammalian cell Anatomy 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 229960002685 biotin Drugs 0.000 abstract 1
- 235000020958 biotin Nutrition 0.000 abstract 1
- 239000011616 biotin Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/70539—MHC-molecules, e.g. HLA-molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
- C12Y603/04015—Biotin-[acetyl-CoA-carboxylase] ligase (6.3.4.15)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
En el presente documento se informa de un método para producir el FcRn biotinilado en el extremo C-terminal que comprende los pasos de cultivar una célula de mamífero que comprende un ácido desoxirribonucleico que codifica el FcRn y la ligasa biotina-[acetil-CoA-carboxilasa] (BirA) de E. coli en un medio que contiene biotina, y recuperar el FcRn biotinilado en el extremo C-terminal de la célula o del medio de cultivo, en donde el ácido desoxirribonucleico que codifica el FcRn y la BirA de E. coli está integrado de manera estable en el genoma de la célula de mamífero y comprende en la dirección 5' a 3' un primer casete de expresión que codifica la proteína similar al complejo principal de histocompatibilidad clase I (a-FcRn) que comprende una etiqueta HisAvi en el extremo C-terminal, un segundo casete de expresión que codifica la ß2-microglobulina (ß2m), un tercer casete de expresión que codifica la proteína similar al complejo principal de histocompatibilidad clase I (a-FcRn) que comprende una etiqueta HisAvi en el extremo C-terminal, un cuarto casete de expresión que codifica la ß2-microglobulina (ß2m), y un quinto casete de expresión que codifica la ligasa biotina-[acetil-CoA-car boxilasa] de E.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19166030 | 2019-03-29 | ||
| PCT/EP2020/058452 WO2020200983A1 (en) | 2019-03-29 | 2020-03-26 | Method for the generation of an fcrn expressing cell by targeted integration of multiple expression cassettes in a defined organization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2021011837A true MX2021011837A (es) | 2021-10-22 |
Family
ID=66239740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2021011837A MX2021011837A (es) | 2019-03-29 | 2020-03-26 | Método para la generación de una célula que expresa el receptor neonatal de región de fragmento cristalizable (fcrn) mediante la integración dirigida de múltiples casetes de expresión en una organización definida. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20220119482A1 (es) |
| EP (1) | EP3947437A1 (es) |
| JP (1) | JP7250950B2 (es) |
| KR (1) | KR20210134932A (es) |
| CN (1) | CN113661173B (es) |
| AU (1) | AU2020253023B2 (es) |
| BR (1) | BR112021019368A2 (es) |
| CA (1) | CA3130546A1 (es) |
| IL (1) | IL286718A (es) |
| MX (1) | MX2021011837A (es) |
| SG (1) | SG11202109391PA (es) |
| WO (1) | WO2020200983A1 (es) |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU650085B2 (en) | 1990-11-13 | 1994-06-09 | Immunex Corporation | Bifunctional selectable fusion genes |
| AU6953394A (en) | 1993-05-21 | 1994-12-20 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| AU2005263331B8 (en) | 2004-07-20 | 2011-06-16 | Symphogen A/S | Anti-Rhesus D recombinant polyclonal antibody and methods of manufacture |
| WO2007124452A2 (en) * | 2006-04-20 | 2007-11-01 | The Regents Of The University Of California | Methods for expressing multiple sirna and shrna from a single vector |
| US8293533B2 (en) * | 2008-12-19 | 2012-10-23 | E.I. Du Pont De Nemours And Company | Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange |
| WO2013006142A1 (en) | 2011-07-05 | 2013-01-10 | Nanyang Technological University | A novel process and reagent for rapid genetic alterations in eukaryotic cells |
| CA2860600C (en) | 2012-02-15 | 2022-07-26 | F. Hoffmann-La Roche Ag | Fc-receptor based affinity chromatography |
| AU2014246731A1 (en) * | 2013-03-30 | 2015-11-19 | Usha Biotech Limited | Methods and constructs for expressing biologically active proteins in mammalian cells |
| HK1218486A2 (zh) * | 2015-12-09 | 2017-02-17 | Ka Lun Alan Ng | 提高魚生長的手段和方法 |
| SG11201807881VA (en) * | 2016-04-20 | 2018-10-30 | Regeneron Pharma | Compositions and methods for making antibodies based on use of an expression-enhancing locus |
| MX2019010575A (es) | 2017-03-10 | 2019-10-15 | Hoffmann La Roche | Metodo para producir anticuerpos multiespecificos. |
| EP3615678B1 (en) * | 2017-04-28 | 2024-07-31 | F. Hoffmann-La Roche AG | Antibody selection method |
| CN111886244A (zh) | 2017-12-22 | 2020-11-03 | 豪夫迈·罗氏有限公司 | 核酸的靶向整合 |
-
2020
- 2020-03-26 WO PCT/EP2020/058452 patent/WO2020200983A1/en unknown
- 2020-03-26 CA CA3130546A patent/CA3130546A1/en active Pending
- 2020-03-26 KR KR1020217030920A patent/KR20210134932A/ko not_active Application Discontinuation
- 2020-03-26 EP EP20713895.9A patent/EP3947437A1/en active Pending
- 2020-03-26 CN CN202080024510.6A patent/CN113661173B/zh active Active
- 2020-03-26 SG SG11202109391PA patent/SG11202109391PA/en unknown
- 2020-03-26 BR BR112021019368A patent/BR112021019368A2/pt unknown
- 2020-03-26 MX MX2021011837A patent/MX2021011837A/es unknown
- 2020-03-26 JP JP2021557767A patent/JP7250950B2/ja active Active
- 2020-03-26 AU AU2020253023A patent/AU2020253023B2/en not_active Ceased
-
2021
- 2021-09-26 IL IL286718A patent/IL286718A/en unknown
- 2021-09-30 US US17/490,979 patent/US20220119482A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022526556A (ja) | 2022-05-25 |
| EP3947437A1 (en) | 2022-02-09 |
| AU2020253023A1 (en) | 2021-09-23 |
| BR112021019368A2 (pt) | 2021-12-07 |
| SG11202109391PA (en) | 2021-09-29 |
| CA3130546A1 (en) | 2020-10-08 |
| AU2020253023B2 (en) | 2022-07-14 |
| WO2020200983A1 (en) | 2020-10-08 |
| JP7250950B2 (ja) | 2023-04-03 |
| CN113661173B (zh) | 2024-10-01 |
| KR20210134932A (ko) | 2021-11-11 |
| IL286718A (en) | 2021-10-31 |
| CN113661173A (zh) | 2021-11-16 |
| US20220119482A1 (en) | 2022-04-21 |
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