AU4906100A - Assays for caspase activity using green fluorescent proteins - Google Patents

Assays for caspase activity using green fluorescent proteins

Info

Publication number: AU4906100A
Authority: AU; Australia
Prior art keywords: assays; green fluorescent; fluorescent proteins; caspase activity; caspase
Prior art date: 1999-05-27
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

AU49061/00A

Inventor

Robin Cassady

Donald Nicholson

Paul Tawa

Steven Xanthoudakis

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Merck Frosst Canada and Co

Original Assignee

Merck Frosst Canada and Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-05-27

Filing date

2000-05-25

Publication date

2000-12-18

2000-05-25 Application filed by Merck Frosst Canada and Co filed Critical Merck Frosst Canada and Co

2000-12-18 Publication of AU4906100A publication Critical patent/AU4906100A/en

Status Abandoned legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
- G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96466—Cysteine endopeptidases (3.4.22)

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
Physics & Mathematics (AREA)
Tropical Medicine & Parasitology (AREA)
Toxicology (AREA)
Analytical Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Biotechnology (AREA)
Immunology (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Peptides Or Proteins (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

AU49061/00A 1999-05-27 2000-05-25 Assays for caspase activity using green fluorescent proteins Abandoned AU4906100A (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US13628699P	1999-05-27	1999-05-27
US60136286		1999-05-27
PCT/CA2000/000620 WO2000073437A1 (en)	1999-05-27	2000-05-25	Assays for caspase activity using green fluorescent proteins

Publications (1)

Publication Number	Publication Date
AU4906100A true AU4906100A (en)	2000-12-18

Family

ID=22472183

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AU49061/00A Abandoned AU4906100A (en)	1999-05-27	2000-05-25	Assays for caspase activity using green fluorescent proteins

Country Status (5)

Country	Link
EP (1)	EP1185644A1 (en)
JP (1)	JP2003501024A (en)
AU (1)	AU4906100A (en)
CA (1)	CA2374369A1 (en)
WO (1)	WO2000073437A1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2001049874A1 (en) *	2000-01-06	2001-07-12	Caliper Technologies Corp.	Methods and systems for monitoring intracellular binding reactions
AU2001262906A1 (en) *	2000-02-11	2001-08-20	Cellomics, Inc.	Peptide biosensors for anthrax protease
GB0103763D0 (en) *	2001-02-15	2001-04-04	Glaxo Group Ltd	Pak-Gep Construct
GB0111459D0 (en) *	2001-05-10	2001-07-04	Isis Innovation	Universal fluorescent sensors
US6979530B2 (en)	2001-05-21	2005-12-27	Applera Corporation	Peptide conjugates and fluorescence detection methods for intracellular caspase assay
US7354735B2 (en)	2001-05-24	2008-04-08	Hong Kong University Of Science And Technology	Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
AU2003226955A1 (en) *	2002-04-19	2003-11-03	Bioimage A/S	Translocation dependent complementation for drug screening
GB0208989D0 (en)	2002-04-19	2002-05-29	Amersham Biosciences Uk Ltd	Methods for measuring enzyme activity
US7306105B2 (en)	2002-11-12	2007-12-11	Mitsubishi Rayon Co., Ltd.	Composite porous membrane and method for producing the same
DE60331580D1 (en)	2002-11-18	2010-04-15	Panomics Inc	CAGED SENSORS, REGULATORS AND COMPOUNDS AND USES THEREOF
JP4214206B2 (en) *	2002-12-10	2009-01-28	独立行政法人理化学研究所	Fluorescent indicator using FRET
DE602004009292T2 (en) *	2003-02-21	2009-03-19	Biotecnol S.A.	USE OF CASPASES FOR THE PRODUCTION OF TIRES OF RECOMBINANT FUSION PROTEINS
DE10318048A1 (en) *	2003-04-17	2004-11-04	Axaron Bioscience Ag	Non-fluorescent reporter proteins that can be activated by proteolysis for fluorescence and their use for the detection of protease-dependent events
WO2004111249A2 (en) *	2003-06-13	2004-12-23	Polymun Scientific Immunbiologische Forschung Gmbh	Protein constructs containing caspase recognition sites
FR2857098B1 (en) *	2003-07-04	2006-05-05	Inst Nat Sante Rech Med	METHOD FOR THE EVIDENCE OF A MOLECULAR EVENT IN A CELL USING FLUORESCENT MARKER PROTEINS
FR2858177A1 (en) *	2003-07-28	2005-02-04	Genethon	USE OF FREQUENT PHENOMENA, DETECTED BY MPLSM, FOR IN VIVO MONITORING OF BIOLOGICAL EVENTS
EP1689877A1 (en) *	2003-11-24	2006-08-16	Novo Nordisk A/S	Fusion proteins and methods of cleavage of such proteins
WO2007135367A2 (en) *	2006-05-19	2007-11-29	University Of Sheffield	Screen for inflammatory response molulators
WO2008104271A2 (en)	2007-02-28	2008-09-04	Sanofi-Aventis	Imaging probes
JP2010535167A (en) *	2007-08-03	2010-11-18	サノフィ−アベンティス	Caspase imaging probe
JP5627569B2 (en) *	2008-04-30	2014-11-19	シーメンスメディカルソリューションズユーエスエーインコーポレイテッドＳｉｅｍｅｎｓＭｅｄｉｃａｌＳｏｌｕｔｉｏｎｓＵＳＡ，Ｉｎｃ．	PET contrast agent based on a novel substrate
EP2646059A4 (en) *	2010-12-01	2016-07-27	Methodist Hospital System	Protease degradable polypeptides and uses thereof
US9439976B2 (en)	2013-02-13	2016-09-13	The Methodist Hospital System	Compositions and methods for using cathepsin E cleavable substrates
GB201318729D0 (en) *	2013-10-23	2013-12-04	Mologic Ltd	Indicator molecules for use in detecting enzyme cleavage activity
US10941435B2 (en)	2016-06-23	2021-03-09	Life Technologies Corporation	Methods and compositions for detecting or measuring caspases or apoptosis

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6803188B1 (en) *	1996-01-31	2004-10-12	The Regents Of The University Of California	Tandem fluorescent protein constructs
PL341661A1 (en) *	1997-10-10	2001-04-23	Cytovia	Novel fluorescent reporter particles and their application in combination with testing capsizes

2000
- 2000-05-25 AU AU49061/00A patent/AU4906100A/en not_active Abandoned
- 2000-05-25 WO PCT/CA2000/000620 patent/WO2000073437A1/en not_active Application Discontinuation
- 2000-05-25 JP JP2001500750A patent/JP2003501024A/en not_active Withdrawn
- 2000-05-25 CA CA002374369A patent/CA2374369A1/en not_active Abandoned
- 2000-05-25 EP EP00930934A patent/EP1185644A1/en not_active Withdrawn

Also Published As

Publication number	Publication date
WO2000073437A1 (en)	2000-12-07
CA2374369A1 (en)	2000-12-07
EP1185644A1 (en)	2002-03-13
JP2003501024A (en)	2003-01-14

Publication	Publication Date	Title
AU4906100A (en)	2000-12-18	Assays for caspase activity using green fluorescent proteins
AU5429199A (en)	2000-02-28	Modified green fluorescent protein
AU5275100A (en)	2000-12-12	Fluorescent protein indicators
AU4488200A (en)	2001-09-17	Modified protein matrices
AU1290001A (en)	2001-05-30	Antibodies
AU2634600A (en)	2000-08-18	Pipetter
AU6516299A (en)	2000-05-01	Protein conjugates containing multimers of green fluorescent protein
AU1227701A (en)	2001-05-08	Fluorescent capture assay for kinase activity
AU2001271589A1 (en)	2002-01-14	Mammalian secreted proteins
AU4935000A (en)	2000-12-05	Antibodies
AU6045900A (en)	2000-12-12	Interaction-activated proteins
AU2880400A (en)	2000-08-29	Tnf-related proteins
AU2445601A (en)	2001-07-03	Mammalian protein phosphatases
AU3162500A (en)	2000-09-21	Homogeneous fluorescence assay
AU3178200A (en)	2000-10-04	Genetic analysis
AU2002354502A1 (en)	2003-07-09	Fluorescent proteins
AU2452301A (en)	2001-07-03	Protein methylarginine-specific antibodies
AU1881200A (en)	2000-08-29	Novel proteins
AU4214800A (en)	2000-10-23	Vesicle associated proteins
AU1362101A (en)	2001-05-14	Novel traf family proteins
AU2061201A (en)	2001-06-25	Mammalian dishevelled-associated proteins
AU4245500A (en)	2000-11-02	Protein transport assays
AU1540701A (en)	2001-06-12	Novel protein
AU2390800A (en)	2000-07-12	Secreted proteins
AU7304600A (en)	2001-04-24	Novel protein

Legal Events

Date	Code	Title	Description
2002-04-11	MK6	Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase