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ATE553144T1 - Partikel und ihre verwendung in einem verfahren zur nukleinsäureisolierung oder einem verfahren zur phosphorproteinisolierung - Google Patents

Partikel und ihre verwendung in einem verfahren zur nukleinsäureisolierung oder einem verfahren zur phosphorproteinisolierung

Info

Publication number
ATE553144T1
ATE553144T1 AT07865890T AT07865890T ATE553144T1 AT E553144 T1 ATE553144 T1 AT E553144T1 AT 07865890 T AT07865890 T AT 07865890T AT 07865890 T AT07865890 T AT 07865890T AT E553144 T1 ATE553144 T1 AT E553144T1
Authority
AT
Austria
Prior art keywords
isolation process
particles
nucleic acid
phosphoric
protein
Prior art date
Application number
AT07865890T
Other languages
English (en)
Inventor
Geir Fonnum
Torkel Stene
Nini Kjus
Stine Bergholtz
Robert Pope
Xiquan Liang
Finn Kirpekar
Kasper Engholm-Keller
Martin Larsen
Ole Jensen
Original Assignee
Invitrogen Dynal As
Univ Southern Denmark
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0625626A external-priority patent/GB0625626D0/en
Priority claimed from GB0625652A external-priority patent/GB0625652D0/en
Priority claimed from GB0625650A external-priority patent/GB0625650D0/en
Application filed by Invitrogen Dynal As, Univ Southern Denmark filed Critical Invitrogen Dynal As
Application granted granted Critical
Publication of ATE553144T1 publication Critical patent/ATE553144T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00Processes of treating or compounding macromolecular substances
    • C08J3/12Powdering or granulating
    • C08J3/128Polymer particles coated by inorganic and non-macromolecular organic compounds
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J9/00Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
    • C08J9/36After-treatment
    • C08J9/365Coating
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326Magnetic particles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01FMAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
    • H01F1/00Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
    • H01F1/01Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials
    • H01F1/03Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity
    • H01F1/032Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials
    • H01F1/04Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys
    • H01F1/06Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
    • H01F1/061Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder with a protective layer
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01FMAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
    • H01F1/00Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
    • H01F1/01Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials
    • H01F1/03Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity
    • H01F1/032Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials
    • H01F1/04Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys
    • H01F1/06Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
    • H01F1/063Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder with a non magnetic core
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2201/00Foams characterised by the foaming process
    • C08J2201/02Foams characterised by the foaming process characterised by mechanical pre- or post-treatments
    • C08J2201/038Use of an inorganic compound to impregnate, bind or coat a foam, e.g. waterglass
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2325/00Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring; Derivatives of such polymers
    • C08J2325/02Homopolymers or copolymers of hydrocarbons
    • C08J2325/04Homopolymers or copolymers of styrene
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08JWORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2333/00Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
    • C08J2333/04Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters
    • C08J2333/06Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters of esters containing only carbon, hydrogen, and oxygen, the oxygen atom being present only as part of the carboxyl radical
    • C08J2333/10Homopolymers or copolymers of methacrylic acid esters
    • C08J2333/12Homopolymers or copolymers of methyl methacrylate

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Polymers & Plastics (AREA)
  • Power Engineering (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Inorganic Compounds Of Heavy Metals (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
AT07865890T 2006-12-21 2007-12-19 Partikel und ihre verwendung in einem verfahren zur nukleinsäureisolierung oder einem verfahren zur phosphorproteinisolierung ATE553144T1 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US87144306P 2006-12-21 2006-12-21
US87142906P 2006-12-21 2006-12-21
US87144006P 2006-12-21 2006-12-21
GB0625626A GB0625626D0 (en) 2006-12-21 2006-12-21 Particles
GB0625652A GB0625652D0 (en) 2006-12-21 2006-12-21 Method for isolating nucleic acid
GB0625650A GB0625650D0 (en) 2006-12-21 2006-12-21 Method for isolating nucleic acid
PCT/US2007/088235 WO2008079905A1 (en) 2006-12-21 2007-12-19 Particles and their use in a method for isolating nucleic acid or a method for isolating phosphoproteins

Publications (1)

Publication Number Publication Date
ATE553144T1 true ATE553144T1 (de) 2012-04-15

Family

ID=42559089

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07865890T ATE553144T1 (de) 2006-12-21 2007-12-19 Partikel und ihre verwendung in einem verfahren zur nukleinsäureisolierung oder einem verfahren zur phosphorproteinisolierung

Country Status (6)

Country Link
US (1) US20100207051A1 (de)
EP (1) EP2066709B1 (de)
JP (1) JP2011503244A (de)
CN (1) CN101627065B (de)
AT (1) ATE553144T1 (de)
WO (1) WO2008079905A1 (de)

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US8445412B2 (en) 2009-02-25 2013-05-21 Illumina, Inc. Separation of pyrophosphate release and pyrophosphate detection
GB0907372D0 (en) 2009-04-29 2009-06-10 Invitrogen Dynal As Particles
JP5346261B2 (ja) * 2009-09-11 2013-11-20 積水化学工業株式会社 金属複合有機樹脂粒子、金属複合有機樹脂粒子の製造方法
JP5453027B2 (ja) * 2009-09-18 2014-03-26 積水化学工業株式会社 金属複合有機樹脂粒子、金属複合有機樹脂粒子の製造方法
US10079139B2 (en) * 2011-03-17 2018-09-18 Kent J. Voorhees Metal oxide laser ionization-mass spectrometry
CN102229925B (zh) * 2011-05-13 2013-04-17 薛昱 一种增强的磁珠法核酸提取方法
KR102019297B1 (ko) 2012-02-09 2019-09-06 라이프 테크놀로지스 코포레이션 친수성 중합체성 입자 및 그의 제조 방법
CN108103147B (zh) 2012-02-09 2021-06-01 生命技术公司 缀合的聚合物颗粒及其制备方法
CN104203964B (zh) 2012-02-09 2017-07-18 生命技术公司 疏水的二丙烯酰胺化合物
EP2664914A1 (de) * 2012-05-16 2013-11-20 Koninklijke Philips N.V. Magnetisch unterstützte Verarbeitung eines Mediums
JP6525341B2 (ja) * 2013-09-09 2019-06-05 ラボ − オン − ア − ビード エービー 磁気分離のための新規な方法及びシステム
CN103439174A (zh) * 2013-09-18 2013-12-11 武汉大学 以二氧化钛为固相对样品中核苷的萃取方法
US9868826B2 (en) 2015-07-02 2018-01-16 Life Technologies Corporation Polymer substrates formed from carboxy functional acrylamide
CN107922974B (zh) 2015-07-02 2021-11-09 生命技术公司 羧基官能亲水微珠的偶合
CN108350490B (zh) 2015-07-06 2022-06-21 生命技术公司 用于测序的底物和方法
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MA46053A (fr) 2016-08-26 2019-07-03 Juno Therapeutics Inc Procédés de dénombrement de particules présentes dans une composition cellulaire
SG11202000606TA (en) 2017-07-29 2020-02-27 Juno Therapeutics Inc Reagents for expanding cells expressing recombinant receptors
KR20200136409A (ko) 2018-02-28 2020-12-07 주노 쎄러퓨티크스 인코퍼레이티드 세포 조성물에 존재하는 입자를 검출하는 방법
US11591591B2 (en) * 2019-08-21 2023-02-28 New England Biolabs, Inc. Isolation of high molecular weight DNA using beads
TWI733302B (zh) * 2020-01-09 2021-07-11 創想生物科技有限公司 核酸分離方法及其系統
CN112321870B (zh) * 2020-11-06 2022-11-18 浙江工商大学 一种微针贴片及其制备方法和应用
CN112807744A (zh) * 2021-01-18 2021-05-18 南京工业大学 一种富集农兽药残留的磁性分散固相萃取材料及其制备方法
CN117321200A (zh) 2021-03-22 2023-12-29 朱诺治疗学股份有限公司 评估病毒载体颗粒效力的方法
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Also Published As

Publication number Publication date
CN101627065A (zh) 2010-01-13
US20100207051A1 (en) 2010-08-19
EP2066709A1 (de) 2009-06-10
EP2066709B1 (de) 2012-04-11
EP2066709A4 (de) 2011-01-12
CN101627065B (zh) 2012-05-23
JP2011503244A (ja) 2011-01-27
WO2008079905A1 (en) 2008-07-03

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