ATE553144T1 - Partikel und ihre verwendung in einem verfahren zur nukleinsäureisolierung oder einem verfahren zur phosphorproteinisolierung - Google Patents
Partikel und ihre verwendung in einem verfahren zur nukleinsäureisolierung oder einem verfahren zur phosphorproteinisolierungInfo
- Publication number
- ATE553144T1 ATE553144T1 AT07865890T AT07865890T ATE553144T1 AT E553144 T1 ATE553144 T1 AT E553144T1 AT 07865890 T AT07865890 T AT 07865890T AT 07865890 T AT07865890 T AT 07865890T AT E553144 T1 ATE553144 T1 AT E553144T1
- Authority
- AT
- Austria
- Prior art keywords
- isolation process
- particles
- nucleic acid
- phosphoric
- protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/12—Powdering or granulating
- C08J3/128—Polymer particles coated by inorganic and non-macromolecular organic compounds
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/36—After-treatment
- C08J9/365—Coating
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/01—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials
- H01F1/03—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity
- H01F1/032—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials
- H01F1/04—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys
- H01F1/06—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
- H01F1/061—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder with a protective layer
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/01—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials
- H01F1/03—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity
- H01F1/032—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials
- H01F1/04—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys
- H01F1/06—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
- H01F1/063—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder with a non magnetic core
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2201/00—Foams characterised by the foaming process
- C08J2201/02—Foams characterised by the foaming process characterised by mechanical pre- or post-treatments
- C08J2201/038—Use of an inorganic compound to impregnate, bind or coat a foam, e.g. waterglass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2325/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an aromatic carbocyclic ring; Derivatives of such polymers
- C08J2325/02—Homopolymers or copolymers of hydrocarbons
- C08J2325/04—Homopolymers or copolymers of styrene
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2333/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
- C08J2333/04—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters
- C08J2333/06—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters of esters containing only carbon, hydrogen, and oxygen, the oxygen atom being present only as part of the carboxyl radical
- C08J2333/10—Homopolymers or copolymers of methacrylic acid esters
- C08J2333/12—Homopolymers or copolymers of methyl methacrylate
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Computational Biology (AREA)
- Polymers & Plastics (AREA)
- Power Engineering (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Materials Engineering (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Inorganic Compounds Of Heavy Metals (AREA)
- Processes Of Treating Macromolecular Substances (AREA)
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US87144306P | 2006-12-21 | 2006-12-21 | |
US87142906P | 2006-12-21 | 2006-12-21 | |
US87144006P | 2006-12-21 | 2006-12-21 | |
GB0625626A GB0625626D0 (en) | 2006-12-21 | 2006-12-21 | Particles |
GB0625652A GB0625652D0 (en) | 2006-12-21 | 2006-12-21 | Method for isolating nucleic acid |
GB0625650A GB0625650D0 (en) | 2006-12-21 | 2006-12-21 | Method for isolating nucleic acid |
PCT/US2007/088235 WO2008079905A1 (en) | 2006-12-21 | 2007-12-19 | Particles and their use in a method for isolating nucleic acid or a method for isolating phosphoproteins |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE553144T1 true ATE553144T1 (de) | 2012-04-15 |
Family
ID=42559089
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07865890T ATE553144T1 (de) | 2006-12-21 | 2007-12-19 | Partikel und ihre verwendung in einem verfahren zur nukleinsäureisolierung oder einem verfahren zur phosphorproteinisolierung |
Country Status (6)
Country | Link |
---|---|
US (1) | US20100207051A1 (de) |
EP (1) | EP2066709B1 (de) |
JP (1) | JP2011503244A (de) |
CN (1) | CN101627065B (de) |
AT (1) | ATE553144T1 (de) |
WO (1) | WO2008079905A1 (de) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8105493B2 (en) * | 2007-06-29 | 2012-01-31 | Jnc Corporation | Aggregation and dispersion methods of magnetic particles, separation and detection methods using the same and detection kit |
US8445412B2 (en) | 2009-02-25 | 2013-05-21 | Illumina, Inc. | Separation of pyrophosphate release and pyrophosphate detection |
GB0907372D0 (en) | 2009-04-29 | 2009-06-10 | Invitrogen Dynal As | Particles |
JP5346261B2 (ja) * | 2009-09-11 | 2013-11-20 | 積水化学工業株式会社 | 金属複合有機樹脂粒子、金属複合有機樹脂粒子の製造方法 |
JP5453027B2 (ja) * | 2009-09-18 | 2014-03-26 | 積水化学工業株式会社 | 金属複合有機樹脂粒子、金属複合有機樹脂粒子の製造方法 |
US10079139B2 (en) * | 2011-03-17 | 2018-09-18 | Kent J. Voorhees | Metal oxide laser ionization-mass spectrometry |
CN102229925B (zh) * | 2011-05-13 | 2013-04-17 | 薛昱 | 一种增强的磁珠法核酸提取方法 |
KR102019297B1 (ko) | 2012-02-09 | 2019-09-06 | 라이프 테크놀로지스 코포레이션 | 친수성 중합체성 입자 및 그의 제조 방법 |
CN108103147B (zh) | 2012-02-09 | 2021-06-01 | 生命技术公司 | 缀合的聚合物颗粒及其制备方法 |
CN104203964B (zh) | 2012-02-09 | 2017-07-18 | 生命技术公司 | 疏水的二丙烯酰胺化合物 |
EP2664914A1 (de) * | 2012-05-16 | 2013-11-20 | Koninklijke Philips N.V. | Magnetisch unterstützte Verarbeitung eines Mediums |
JP6525341B2 (ja) * | 2013-09-09 | 2019-06-05 | ラボ − オン − ア − ビード エービー | 磁気分離のための新規な方法及びシステム |
CN103439174A (zh) * | 2013-09-18 | 2013-12-11 | 武汉大学 | 以二氧化钛为固相对样品中核苷的萃取方法 |
US9868826B2 (en) | 2015-07-02 | 2018-01-16 | Life Technologies Corporation | Polymer substrates formed from carboxy functional acrylamide |
CN107922974B (zh) | 2015-07-02 | 2021-11-09 | 生命技术公司 | 羧基官能亲水微珠的偶合 |
CN108350490B (zh) | 2015-07-06 | 2022-06-21 | 生命技术公司 | 用于测序的底物和方法 |
CN115197940A (zh) | 2015-07-14 | 2022-10-18 | 雅培分子公司 | 使用铜-钛氧化物纯化核酸 |
US20180135040A1 (en) | 2016-02-16 | 2018-05-17 | Life Magnetics, Inc. | Methods for separating nucleic acids with graphene coated magnetic beads |
MA46053A (fr) | 2016-08-26 | 2019-07-03 | Juno Therapeutics Inc | Procédés de dénombrement de particules présentes dans une composition cellulaire |
SG11202000606TA (en) | 2017-07-29 | 2020-02-27 | Juno Therapeutics Inc | Reagents for expanding cells expressing recombinant receptors |
KR20200136409A (ko) | 2018-02-28 | 2020-12-07 | 주노 쎄러퓨티크스 인코퍼레이티드 | 세포 조성물에 존재하는 입자를 검출하는 방법 |
US11591591B2 (en) * | 2019-08-21 | 2023-02-28 | New England Biolabs, Inc. | Isolation of high molecular weight DNA using beads |
TWI733302B (zh) * | 2020-01-09 | 2021-07-11 | 創想生物科技有限公司 | 核酸分離方法及其系統 |
CN112321870B (zh) * | 2020-11-06 | 2022-11-18 | 浙江工商大学 | 一种微针贴片及其制备方法和应用 |
CN112807744A (zh) * | 2021-01-18 | 2021-05-18 | 南京工业大学 | 一种富集农兽药残留的磁性分散固相萃取材料及其制备方法 |
CN117321200A (zh) | 2021-03-22 | 2023-12-29 | 朱诺治疗学股份有限公司 | 评估病毒载体颗粒效力的方法 |
AU2022241654A1 (en) | 2021-03-22 | 2023-09-28 | Juno Therapeutics, Inc. | Methods of determining potency of a therapeutic cell composition |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NO155316C (no) * | 1982-04-23 | 1987-03-11 | Sintef | Fremgangsmaate for fremstilling av magnetiske polymerpartikler. |
US4707523A (en) * | 1985-10-11 | 1987-11-17 | California Institute Of Technology | Magnetic particles |
DE3639949A1 (de) * | 1986-11-22 | 1988-06-09 | Diagen Inst Molekularbio | Verfahren zur trennung von langkettigen nukleinsaeuren |
US5091206A (en) * | 1987-10-26 | 1992-02-25 | Baxter Diagnostics Inc. | Process for producing magnetically responsive polymer particles and application thereof |
US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
NL8900725A (nl) * | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
AU1978692A (en) * | 1991-04-12 | 1992-11-17 | Minnesota Mining And Manufacturing Company | Purification of nucleic acids using metal oxide supports |
DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
US5705628A (en) * | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
JP3627342B2 (ja) * | 1996-01-31 | 2005-03-09 | Jsr株式会社 | 磁性ポリマー粒子およびその製造方法 |
US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
AU731812B2 (en) * | 1997-06-10 | 2001-04-05 | Transgenomic, Inc. | System and method for performing polynucleotide separations using liquid chromatography |
US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
GB0116358D0 (en) * | 2001-07-04 | 2001-08-29 | Genovision As | Preparation of polymer particles |
US6797782B2 (en) * | 2002-03-25 | 2004-09-28 | Jsr Corporation | Process for producing particles for diagnostic reagent |
GB0228914D0 (en) * | 2002-12-11 | 2003-01-15 | Dynal Biotech Asa | Particles |
US20060141253A1 (en) * | 2003-01-31 | 2006-06-29 | Yuzuru Sugano | Fine particles having controlled density |
JP4199082B2 (ja) * | 2003-09-22 | 2008-12-17 | 日鉄鉱業株式会社 | 酸化チタン膜被覆粉体およびその製造方法 |
-
2007
- 2007-12-19 CN CN200780051278.XA patent/CN101627065B/zh not_active Expired - Fee Related
- 2007-12-19 US US12/519,746 patent/US20100207051A1/en not_active Abandoned
- 2007-12-19 JP JP2009543199A patent/JP2011503244A/ja active Pending
- 2007-12-19 AT AT07865890T patent/ATE553144T1/de active
- 2007-12-19 WO PCT/US2007/088235 patent/WO2008079905A1/en active Application Filing
- 2007-12-19 EP EP07865890A patent/EP2066709B1/de not_active Not-in-force
Also Published As
Publication number | Publication date |
---|---|
CN101627065A (zh) | 2010-01-13 |
US20100207051A1 (en) | 2010-08-19 |
EP2066709A1 (de) | 2009-06-10 |
EP2066709B1 (de) | 2012-04-11 |
EP2066709A4 (de) | 2011-01-12 |
CN101627065B (zh) | 2012-05-23 |
JP2011503244A (ja) | 2011-01-27 |
WO2008079905A1 (en) | 2008-07-03 |
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