ATE455186T1 - Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren - Google Patents
Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäurenInfo
- Publication number
- ATE455186T1 ATE455186T1 AT01950624T AT01950624T ATE455186T1 AT E455186 T1 ATE455186 T1 AT E455186T1 AT 01950624 T AT01950624 T AT 01950624T AT 01950624 T AT01950624 T AT 01950624T AT E455186 T1 ATE455186 T1 AT E455186T1
- Authority
- AT
- Austria
- Prior art keywords
- amplification
- methods
- transcription
- module
- rna transcripts
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21390800P | 2000-06-26 | 2000-06-26 | |
US27774801P | 2001-03-21 | 2001-03-21 | |
PCT/US2001/020660 WO2002000938A2 (en) | 2000-06-26 | 2001-06-26 | Methods and compositions for transcription-based nucleic acid amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE455186T1 true ATE455186T1 (de) | 2010-01-15 |
Family
ID=26908503
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT01950624T ATE455186T1 (de) | 2000-06-26 | 2001-06-26 | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
Country Status (10)
Country | Link |
---|---|
US (3) | US6686156B2 (de) |
EP (1) | EP1356094B1 (de) |
JP (1) | JP2004513617A (de) |
CN (1) | CN1498274A (de) |
AT (1) | ATE455186T1 (de) |
AU (1) | AU2001271595A1 (de) |
CA (1) | CA2412721A1 (de) |
DE (1) | DE60141087D1 (de) |
MX (1) | MXPA02012735A (de) |
WO (1) | WO2002000938A2 (de) |
Families Citing this family (65)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
ATE455186T1 (de) | 2000-06-26 | 2010-01-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
JP2005503755A (ja) | 2000-12-13 | 2005-02-10 | ニューゲン テクノロジーズ, インコーポレイテッド | 核酸配列の複数コピーを作製するための方法および組成物、ならびにそれらを検出する方法 |
CN100351393C (zh) | 2001-02-15 | 2007-11-28 | 宝生物工程株式会社 | 核苷酸多态性的检测方法 |
CN1289690C (zh) * | 2001-03-09 | 2006-12-13 | 纽亘技术公司 | Rna序列扩增的方法和组合物 |
US7094536B2 (en) * | 2001-03-09 | 2006-08-22 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
US8137911B2 (en) * | 2001-05-22 | 2012-03-20 | Cellscript, Inc. | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
AU2003222269A1 (en) * | 2002-03-11 | 2003-09-29 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination |
US20030219729A1 (en) * | 2002-03-15 | 2003-11-27 | Tosoh Corporation | Unary avian myeloblastosis virus revers transcriptase and its use |
AU2003279697A1 (en) * | 2002-05-17 | 2004-02-16 | Nugen Technologies, Inc. | Methods for fragmentation, labeling and immobilizaton of nucleic acids |
JP3923917B2 (ja) * | 2003-03-26 | 2007-06-06 | 株式会社東芝 | ターゲットの製造方法、標的配列検出方法、ターゲットおよび標的配列検出用アッセイキット |
JP2006523465A (ja) | 2003-04-14 | 2006-10-19 | ニューゲン テクノロジーズ, インコーポレイテッド | ランダムにプライミングされる複合プライマーを用いる大規模増幅 |
CA2552007A1 (en) * | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US7713697B2 (en) * | 2004-08-27 | 2010-05-11 | Gen-Probe Incorporated | Methods and kits for amplifying DNA |
AU2005280162B2 (en) * | 2004-08-27 | 2012-04-26 | Gen-Probe Incorporated | Single-primer nucleic acid amplification methods |
KR100695134B1 (ko) * | 2004-11-25 | 2007-03-14 | 삼성전자주식회사 | 층류를 이용한 마이크로어레이 및 이의 제조 방법 |
EP1836302B1 (de) * | 2004-12-23 | 2010-04-14 | Amersham Biosciences Corp. | Rna-amplifikation auf ligationsbasis |
EP1841879A4 (de) | 2005-01-25 | 2009-05-27 | Population Genetics Technologi | Isothermische dna-amplifikation |
US20070020639A1 (en) * | 2005-07-20 | 2007-01-25 | Affymetrix, Inc. | Isothermal locus specific amplification |
EP1929046B1 (de) | 2005-09-07 | 2012-07-11 | Nugen Technologies, Inc. | Verbessertes nukleinsäureamplifikationsverfahren |
WO2007087262A2 (en) * | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Selective genome amplification |
US20090023597A1 (en) * | 2006-04-12 | 2009-01-22 | Siemens Healthcare Diagnostics Inc. | Single Nucleotide Polymorphism Detection from Unamplified Genomic DNA |
US20090275486A1 (en) * | 2006-05-16 | 2009-11-05 | Nurith Kurn | Nucleic acid separation and purification method based on reversible charge interactions |
US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
CA2656315A1 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
US7501254B2 (en) * | 2006-07-20 | 2009-03-10 | Ghc Technologies, Inc. | Methods and compositions for amplification and capture of nucleic acid sequences |
EP2121956B1 (de) | 2006-12-21 | 2016-08-17 | Gen-Probe Incorporated | Verfahren und zusammensetzungen zur amplizierung von nukleinsäuren |
US8183359B2 (en) * | 2007-03-01 | 2012-05-22 | Gen-Probe Incorporated | Kits for amplifying DNA |
US7794985B2 (en) * | 2007-04-04 | 2010-09-14 | Ghc Technologies, Inc. | Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins |
US9388457B2 (en) | 2007-09-14 | 2016-07-12 | Affymetrix, Inc. | Locus specific amplification using array probes |
US8716190B2 (en) | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
EP2215265B1 (de) | 2007-11-14 | 2013-05-22 | Grifols Therapeutics Inc. | Verfahren zur anpassung der ergebnisse eines polymerasekettenreaktionsinstruments |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
WO2010126913A1 (en) | 2009-04-27 | 2010-11-04 | Gen-Probe Incorporated | Methods and kits for use in the selective amplification of target sequences |
AU2010266234B2 (en) | 2009-07-01 | 2013-07-25 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
EP2475777A4 (de) * | 2009-09-11 | 2013-03-06 | Nugen Technologies Inc | Zusammensetzungen und verfahren für vollständige transkriptomanalyse |
GB2487341A (en) * | 2009-11-02 | 2012-07-18 | Nugen Technologies Inc | Compositions and methods for targeted nucleic acid sequence selection and amplification |
CN102725424B (zh) * | 2010-01-25 | 2014-07-09 | Rd生物科技公司 | 靶核酸的自折叠扩增 |
EP2769007B1 (de) | 2011-10-19 | 2016-12-07 | Nugen Technologies, Inc. | Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren |
EP2798089B1 (de) | 2011-12-30 | 2018-05-23 | Bio-rad Laboratories, Inc. | Verfahren und zusammensetzungen zur durchführung von nucleinsäureamplifikationsreaktionen |
WO2013112923A1 (en) | 2012-01-26 | 2013-08-01 | Nugen Technologies, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
EP2847353B1 (de) | 2012-05-10 | 2022-01-19 | The General Hospital Corporation | Verfahren zur bestimmung einer nukleotidsequenz |
SG11201408478QA (en) | 2012-06-18 | 2015-02-27 | Nugen Technologies Inc | Compositions and methods for negative selection of non-desired nucleic acid sequences |
US9777319B2 (en) | 2012-06-29 | 2017-10-03 | General Electric Company | Method for isothermal DNA amplification starting from an RNA template |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
EP3640348A1 (de) * | 2012-08-30 | 2020-04-22 | Gen-Probe Incorporated | Mehrphasige nukleinsäureamplifikation |
MY167603A (en) | 2012-10-04 | 2018-09-20 | The Board Of Trustees Of Leland Stanford Junior Univ | Method and reagents for detection, quantitation, and serotyping of dengue viruses |
US9410173B2 (en) | 2012-10-24 | 2016-08-09 | Clontech Laboratories, Inc. | Template switch-based methods for producing a product nucleic acid |
US20140274738A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
EP3760733A1 (de) | 2013-10-17 | 2021-01-06 | Takara Bio USA, Inc. | Verfahren zum hinzufügen von adaptern zu nukleinsäuren und zusammensetzungen zur durchführung des verfahrens |
EP3068883B1 (de) | 2013-11-13 | 2020-04-29 | Nugen Technologies, Inc. | Zusammensetzungen und verfahren zur identifizierung einer duplikatsequenzierungslesung |
US9719136B2 (en) | 2013-12-17 | 2017-08-01 | Takara Bio Usa, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
WO2015112974A1 (en) | 2014-01-27 | 2015-07-30 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
WO2016022833A1 (en) | 2014-08-06 | 2016-02-11 | Nugen Technologies, Inc. | Digital measurements from targeted sequencing |
EP3933039A1 (de) | 2016-09-15 | 2022-01-05 | ArcherDX, LLC | Verfahren für die zubereitung von nukleinsäureproben |
EP3512965B1 (de) | 2016-09-15 | 2024-03-13 | ArcherDX, LLC | Verfahren zur herstellung von nukleinsäuren zur analyse von zellfreier dna |
ES2927463T3 (es) * | 2016-12-09 | 2022-11-07 | Broad Inst Inc | Diagnósticos basados en el sistema efector CRISPR |
CN106529206B (zh) * | 2016-12-20 | 2019-02-22 | 大连海事大学 | 一种蛋白质二维结构图功能元件的自动布线方法 |
EP3596218B1 (de) * | 2017-03-15 | 2023-08-23 | The Broad Institute, Inc. | Auf crispr-effektorsystem basierende diagnostik für virusnachweis |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
CN113528624A (zh) * | 2020-04-17 | 2021-10-22 | 青岛大学 | 扩增和检测核酸的方法及试剂盒 |
CN113275053A (zh) | 2020-02-03 | 2021-08-20 | 帝肯基因组学公司 | 试剂存储系统 |
Family Cites Families (107)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57501692A (de) | 1980-09-24 | 1982-09-16 | ||
US4582788A (en) | 1982-01-22 | 1986-04-15 | Cetus Corporation | HLA typing method and cDNA probes used therein |
ATE53862T1 (de) | 1982-01-22 | 1990-06-15 | Cetus Corp | Verfahren zur charakterisierung von hla und die darin benutzten cdns-testmittel. |
US4786600A (en) | 1984-05-25 | 1988-11-22 | The Trustees Of Columbia University In The City Of New York | Autocatalytic replication of recombinant RNA |
US4683194A (en) | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
CA1284931C (en) | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
DE3634356A1 (de) | 1986-10-08 | 1988-04-21 | Epis Sa | Arzneimittel enthaltend alpha-halogenierte dicarbonsaeuren |
IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
JP2774121B2 (ja) | 1987-07-31 | 1998-07-09 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | 標的ポリヌクレオチド配列の選択的増幅 |
US6090591A (en) | 1987-07-31 | 2000-07-18 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
US6004745A (en) | 1987-09-21 | 1999-12-21 | Gen-Probe Incorporated | Hybridization protection assay |
US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
AU622426B2 (en) | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
JP2650159B2 (ja) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
AU623642B2 (en) | 1988-03-24 | 1992-05-21 | University Of Iowa Research Foundation, The | Catalytic hybridization systems for the detection of nucleic acid sequences based on their activity as cofactors in catalytic reactions in which a complementary labeled nucleic acid probe is cleaved |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
US5508178A (en) | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US6040138A (en) * | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
US5766849A (en) | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
EP0731174B1 (de) | 1989-07-11 | 2004-11-17 | Gen-Probe Incorporated | Verfahren zur Amplifikation von Nukleinsäuresequenzen |
US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US6013431A (en) | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
CA2037349C (en) | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
HU218095B (hu) | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
US5427911A (en) | 1990-05-01 | 1995-06-27 | Yale University | Coupled amplification and sequencing of DNA |
US5194370A (en) | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
US5595891A (en) | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
US5846710A (en) | 1990-11-02 | 1998-12-08 | St. Louis University | Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
AU8997991A (en) | 1991-01-31 | 1992-08-06 | Becton Dickinson & Company | Exonuclease mediated strand displacement amplification |
US5888819A (en) | 1991-03-05 | 1999-03-30 | Molecular Tool, Inc. | Method for determining nucleotide identity through primer extension |
US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
US5169766A (en) | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
DE69332665T2 (de) | 1992-03-11 | 2003-11-27 | Dana-Farber Cancer Institute, Inc. | Methode um mrna zu klonieren |
KR100249110B1 (ko) | 1992-05-06 | 2000-04-01 | 다니엘 엘. 캐시앙 | 핵산 서열 증폭 방법, 이에 이용되는 조성물 및 키트 |
US5710028A (en) | 1992-07-02 | 1998-01-20 | Eyal; Nurit | Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor |
EP0652973B1 (de) | 1992-07-31 | 1997-04-23 | BEHRINGWERKE Aktiengesellschaft | Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden |
ZA936016B (en) | 1992-08-24 | 1994-03-10 | Akzo Nv | Method for nucleic acid amplification |
US5591575A (en) | 1993-04-07 | 1997-01-07 | Amersham International Plc | Subtraction hybridization employing aziridinylbenoquinone cross-linking agents |
US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
JPH0723799A (ja) | 1993-07-13 | 1995-01-27 | Hitachi Ltd | ポリヌクレオチドの検出方法 |
US6027923A (en) | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
JPH09506762A (ja) | 1993-08-18 | 1997-07-08 | アイディー バイオメディカル コーポレイション | 近接配列−酵素分子を利用して標的核酸配列を検出するための組成物および方法 |
AU8102694A (en) | 1993-11-17 | 1995-06-06 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
US6060288A (en) * | 1994-08-03 | 2000-05-09 | Mosaic Technologies | Method for performing amplification of nucleic acid on supports |
FR2724934B1 (fr) * | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
US5556752A (en) | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
US5665545A (en) | 1994-11-28 | 1997-09-09 | Akzo Nobel N.V. | Terminal repeat amplification method |
US5556771A (en) * | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
US5763178A (en) | 1995-06-07 | 1998-06-09 | Trevigen, Inc. | Oscillating signal amplifier for nucleic acid detection |
US5882867A (en) | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
FR2737223B1 (fr) | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
US5871697A (en) | 1995-10-24 | 1999-02-16 | Curagen Corporation | Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
US6143495A (en) | 1995-11-21 | 2000-11-07 | Yale University | Unimolecular segment amplification and sequencing |
EP0866868B1 (de) | 1995-12-15 | 2001-07-11 | Amersham Life Science Inc | Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität |
US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
GB9604267D0 (en) | 1996-02-29 | 1996-05-01 | Royal Infirmary Of Edinburgh N | Mutation assay |
EP1736554B1 (de) | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Bestimmung von Unterschieden in der Nukleinsäuresequenz mittels einer Kombination von Ligasebestimmung und Polymerasekettenreaktion |
US6218105B1 (en) * | 1996-07-19 | 2001-04-17 | Kathleen S. Hall | High throughput papilloma virus in vitro infectivity assay |
EP0942917B1 (de) | 1996-08-14 | 2015-02-25 | Life Technologies Corporation | Verwendung von stabile zubereitungen in nukleinsäureamplifikation und sequenzierung verfahren |
DE19653439A1 (de) | 1996-12-20 | 1998-07-02 | Svante Dr Paeaebo | Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung |
ATE347615T1 (de) * | 1997-04-16 | 2006-12-15 | Applera Corp | Nukleinsäuresammlung |
US6140086A (en) * | 1997-08-15 | 2000-10-31 | Fox; Donna K. | Methods and compositions for cloning nucleic acid molecules |
JP4317953B2 (ja) * | 1998-01-22 | 2009-08-19 | 独立行政法人理化学研究所 | Dnaの塩基配列決定方法 |
US20010000077A1 (en) | 1998-02-03 | 2001-03-29 | Engelhardt Dean L. | Novel process, construct and conjugate for producing multiple nucleic acid copies |
US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
US6358712B1 (en) | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
WO2000070095A2 (en) * | 1999-05-17 | 2000-11-23 | Dade Behring Inc. | Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide |
US6132997A (en) | 1999-05-28 | 2000-10-17 | Agilent Technologies | Method for linear mRNA amplification |
US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
ATE262595T1 (de) * | 1999-09-13 | 2004-04-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen |
US6300073B1 (en) * | 1999-10-01 | 2001-10-09 | Clontech Laboratories, Inc. | One step RT-PCR methods, enzyme mixes and kits for use in practicing the same |
AU2001251069A1 (en) * | 2000-03-28 | 2001-10-08 | The Government of the United States of America, as represented by the Secretary, Department of Health and Human Services, The NationalInstitutes Of Health | Gene profiling arrays |
ATE455186T1 (de) | 2000-06-26 | 2010-01-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
AU1309602A (en) | 2000-10-06 | 2002-04-15 | Nugen Technologies Inc | Methods and probes for detection and/or quantification of nucleic acid sequences |
US6673549B1 (en) * | 2000-10-12 | 2004-01-06 | Incyte Corporation | Genes expressed in C3A liver cell cultures treated with steroids |
AR031640A1 (es) * | 2000-12-08 | 2003-09-24 | Applied Research Systems | Amplificacion isotermica de acidos nucleicos en un soporte solido |
CN1289690C (zh) | 2001-03-09 | 2006-12-13 | 纽亘技术公司 | Rna序列扩增的方法和组合物 |
US7094536B2 (en) | 2001-03-09 | 2006-08-22 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
US7056671B2 (en) * | 2001-08-20 | 2006-06-06 | Takara Bio Inc. | Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide |
US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
AU2002352858A1 (en) * | 2001-11-20 | 2003-06-10 | Exact Sciences Corporation | Automated sample preparation methods and devices |
AU2003220249A1 (en) * | 2002-03-15 | 2003-09-29 | Arcturus Bioscience, Inc. | Improved nucleic acid amplification |
CN101128601B (zh) * | 2003-01-29 | 2011-06-08 | 454生命科学公司 | 核酸扩增和测序的方法 |
US20040241732A1 (en) * | 2003-04-11 | 2004-12-02 | Chu-An Chang | Method of generating long nucleic acid molecules of defined sequence |
CA2552007A1 (en) * | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US20070054301A1 (en) * | 2005-09-06 | 2007-03-08 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
US20090275486A1 (en) * | 2006-05-16 | 2009-11-05 | Nurith Kurn | Nucleic acid separation and purification method based on reversible charge interactions |
CA2656315A1 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
-
2001
- 2001-06-26 AT AT01950624T patent/ATE455186T1/de not_active IP Right Cessation
- 2001-06-26 WO PCT/US2001/020660 patent/WO2002000938A2/en active Application Filing
- 2001-06-26 DE DE60141087T patent/DE60141087D1/de not_active Expired - Lifetime
- 2001-06-26 MX MXPA02012735A patent/MXPA02012735A/es not_active Application Discontinuation
- 2001-06-26 CN CNA018132979A patent/CN1498274A/zh active Pending
- 2001-06-26 US US09/893,191 patent/US6686156B2/en not_active Expired - Fee Related
- 2001-06-26 EP EP01950624A patent/EP1356094B1/de not_active Expired - Lifetime
- 2001-06-26 CA CA002412721A patent/CA2412721A1/en not_active Abandoned
- 2001-06-26 JP JP2002506251A patent/JP2004513617A/ja not_active Withdrawn
- 2001-06-26 AU AU2001271595A patent/AU2001271595A1/en not_active Abandoned
-
2003
- 2003-10-14 US US10/686,466 patent/US7294461B2/en not_active Expired - Fee Related
-
2007
- 2007-10-31 US US11/933,332 patent/US20080182300A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CA2412721A1 (en) | 2002-01-03 |
WO2002000938A9 (en) | 2003-07-03 |
WO2002000938A2 (en) | 2002-01-03 |
AU2001271595A1 (en) | 2002-01-08 |
MXPA02012735A (es) | 2004-04-20 |
US20080182300A1 (en) | 2008-07-31 |
US20020058270A1 (en) | 2002-05-16 |
US6686156B2 (en) | 2004-02-03 |
EP1356094A2 (de) | 2003-10-29 |
JP2004513617A (ja) | 2004-05-13 |
US7294461B2 (en) | 2007-11-13 |
US20040203019A1 (en) | 2004-10-14 |
CN1498274A (zh) | 2004-05-19 |
WO2002000938A3 (en) | 2003-08-28 |
EP1356094B1 (de) | 2010-01-13 |
DE60141087D1 (de) | 2010-03-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE455186T1 (de) | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren | |
DE60009323D1 (de) | Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen | |
EP4407041A3 (de) | Verwendung von random-rpiming zur gewinnung von v(d)j-informationen in voller länge für immunrepertoiresequenzierung | |
DE69928263D1 (de) | Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen | |
DE60335637D1 (de) | Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren | |
ATE340857T1 (de) | Verfahren zur herstellung von l-aminosäuren durch erhöhung des zellulären nadph | |
DE602006014009D1 (de) | Nukleinsäure konstrukten, pharmazeutische kompositionen und methoden für die behandlung von krebs | |
EA201891629A1 (ru) | Способы и композиции для повышения эффективности нацеленной модификации генов с применением опосредуемой олигонуклеотидами репарации генов | |
CA2371672A1 (en) | A method for the determination of a nucleic acid using a control | |
BR9808368A (pt) | Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse. | |
ATE475720T1 (de) | Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben | |
TW200724677A (en) | Method for Mesenchymal stem cell identification by use of molecular marker and use thereof | |
WO2006102309A3 (en) | Methods, compositions, and kits for detection of micro rna | |
CL2009001601A1 (es) | Secuencia reguladora aisladas capaces de iniciar y conducir la transcripcion de un polinucleotido heterólogo en plantas, construccion génica que comprende dichos acidos nucleicos y metodo para producir una planta con un fenotipo modificado. (divisional solicitud n° 1425-05) | |
SE0301951D0 (sv) | New method | |
ATE409235T1 (de) | Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen | |
ATE490319T1 (de) | Auswahl und anreicherung von proteinen durch in- vitro-untergliederung | |
DE602005017252D1 (de) | Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates | |
AR014528A1 (es) | Adn que comprende una secuencia de nucleotidos que se puede traducir a una proteina con actividad de invertasa, proteina derivada del adn que tieneactividad de invertasa pero que carece de actividad beta-fructofuranosidasa; metodo para producir adn y dicho metodo donde el adn es producido por una | |
SG11201811799VA (en) | Primer set, kit and method for detecting two or more target nucleic acids | |
RU2008149510A (ru) | Набор праймеров для использования при детекции дрожжей рода dekkera и дрожжей рода brettanomyces | |
EP4403648A3 (de) | Verfahren und zusammensetzungen zur manipulation von nukleinsäuren | |
DE50313095D1 (de) | Sputterverfahren zur herstellung von eigenspannungsoptimierten beschichtungen | |
WO2023086938A3 (en) | Type v nucleases | |
AR047590A1 (es) | Alteracion de la estructura de la raiz durante el desarrollo de la planta |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |