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ATE260987T1 - Verfahren zum screening enzymatischer aktivität - Google Patents

Verfahren zum screening enzymatischer aktivität

Info

Publication number
ATE260987T1
ATE260987T1 AT96942920T AT96942920T ATE260987T1 AT E260987 T1 ATE260987 T1 AT E260987T1 AT 96942920 T AT96942920 T AT 96942920T AT 96942920 T AT96942920 T AT 96942920T AT E260987 T1 ATE260987 T1 AT E260987T1
Authority
AT
Austria
Prior art keywords
enzymatic activity
dna
screening
activity
screening enzymatic
Prior art date
Application number
AT96942920T
Other languages
English (en)
Inventor
Jay M Short
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/651,568 external-priority patent/US5939250A/en
Application filed by Diversa Corp filed Critical Diversa Corp
Application granted granted Critical
Publication of ATE260987T1 publication Critical patent/ATE260987T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811Selection methods for production or design of target specific oligonucleotides or binding molecules
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03001Alkaline phosphatase (3.1.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021Beta-glucosidase (3.2.1.21)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Ecology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT96942920T 1995-12-07 1996-12-06 Verfahren zum screening enzymatischer aktivität ATE260987T1 (de)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US831695P 1995-12-07 1995-12-07
US831795P 1995-12-07 1995-12-07
US831195P 1995-12-07 1995-12-07
US08/651,568 US5939250A (en) 1995-12-07 1996-05-22 Production of enzymes having desired activities by mutagenesis
US08/692,002 US6054267A (en) 1995-12-07 1996-08-02 Method for screening for enzyme activity
PCT/US1996/019457 WO1997020918A1 (en) 1995-12-07 1996-12-06 Method of screening for enzyme activity

Publications (1)

Publication Number Publication Date
ATE260987T1 true ATE260987T1 (de) 2004-03-15

Family

ID=27533271

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96942920T ATE260987T1 (de) 1995-12-07 1996-12-06 Verfahren zum screening enzymatischer aktivität

Country Status (8)

Country Link
EP (2) EP0866853B1 (de)
JP (2) JP2000501606A (de)
AT (1) ATE260987T1 (de)
AU (1) AU720334B2 (de)
CA (1) CA2239686A1 (de)
DE (1) DE69631787T2 (de)
IL (1) IL124794A (de)
WO (1) WO1997020918A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
EP1950288A1 (de) * 1996-01-11 2008-07-30 Verenium Corporation Thermotoga-Endoglucanase
ATE271126T1 (de) * 1996-01-11 2004-07-15 Diversa Corp Glykosidaseenzyme
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US5789228A (en) * 1996-05-22 1998-08-04 Diversa Corporation Endoglucanases
US7465571B1 (en) 1996-05-22 2008-12-16 Verenium Corporation Endoglucanases
IL130245A (en) 1996-12-06 2007-06-17 Verenium Corp Glycosidase enzymes
US7279316B2 (en) 1996-12-06 2007-10-09 Verenium Corporation Enzymes having glycosidase activity and methods of use thereof
WO1998059075A1 (en) 1997-06-25 1998-12-30 Life Technologies, Inc. Improved method for isolating and recovering target dna or rna molecules having a desired nucleotide sequence
DE19731990A1 (de) * 1997-07-25 1999-01-28 Studiengesellschaft Kohle Mbh Verfahren zur Herstellung und Identifizierung von neuen Hydrolasen mit verbesserten Eigenschaften
US6261842B1 (en) 1997-10-23 2001-07-17 Wisconsin Alumni Research Foundation Microorganism genomics, compositions and methods related thereto
CA2329122A1 (en) * 1999-03-26 2000-10-05 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
JP2003521934A (ja) 2000-02-09 2003-07-22 ジェンベク、インコーポレイティッド ウイルスベクターライブラリーの製造および使用方法
AU2001278872A1 (en) 2000-07-06 2002-01-21 Genvec, Inc. Method of identifying a binding partner of a gene product
JP4114075B2 (ja) * 2004-02-16 2008-07-09 ヒューマン・メタボローム・テクノロジーズ株式会社 遺伝子産物の機能同定方法及び結合物質同定方法
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
JP4568571B2 (ja) * 2004-10-06 2010-10-27 ユニバーサル・バイオ・リサーチ株式会社 熱安定性又は熱活性dnaポリメラーゼ及びそれをコードするdna
MX355507B (es) 2009-03-09 2018-04-19 Bioatla Llc Proteinas mirac.
ES2966088T3 (es) * 2010-07-16 2024-04-18 Bioatla Inc Nuevos métodos de evolución de proteínas
CN109508300B (zh) * 2018-11-22 2022-07-05 广州爱云信息技术有限公司 一种磁盘碎片整理方法、设备及计算机可读存储介质

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU598516B2 (en) * 1985-12-17 1990-06-28 Lubrizol Genetics Inc. Isolation of genes for biosynthesis of polyketide antibiotics
ATE157702T1 (de) * 1990-02-07 1997-09-15 Abbott Lab Alkalische phosphatase mit verbesserter spezifischer aktivität zur verwendung in indikatorreagenzien
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
EP0548228B1 (de) * 1990-09-13 1998-08-12 Novo Nordisk A/S Lipase-varianten
JPH067160A (ja) * 1991-12-18 1994-01-18 New England Biolabs Inc 古細菌からの組換え熱安定性dnaポリメラーゼ
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms

Also Published As

Publication number Publication date
IL124794A (en) 2008-06-05
WO1997020918A1 (en) 1997-06-12
EP0866853A1 (de) 1998-09-30
IL124794A0 (en) 1999-01-26
AU720334B2 (en) 2000-05-25
DE69631787T2 (de) 2005-03-10
CA2239686A1 (en) 1997-06-12
EP0866853A4 (de) 1999-09-22
EP1130090A2 (de) 2001-09-05
EP1130090A3 (de) 2003-11-26
JP2000501606A (ja) 2000-02-15
JP2001078786A (ja) 2001-03-27
EP0866853B1 (de) 2004-03-03
AU1148997A (en) 1997-06-27
DE69631787D1 (de) 2004-04-08

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