[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

DE69637683D1 - Verfahren zum Einführen von Nukleinsäuren in Zellen für therapeutische und diagnostische Verwendungen - Google Patents

Verfahren zum Einführen von Nukleinsäuren in Zellen für therapeutische und diagnostische Verwendungen

Info

Publication number
DE69637683D1
DE69637683D1 DE69637683T DE69637683T DE69637683D1 DE 69637683 D1 DE69637683 D1 DE 69637683D1 DE 69637683 T DE69637683 T DE 69637683T DE 69637683 T DE69637683 T DE 69637683T DE 69637683 D1 DE69637683 D1 DE 69637683D1
Authority
DE
Germany
Prior art keywords
therapeutic
cells
nucleic acids
introducing nucleic
diagnostic uses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69637683T
Other languages
English (en)
Inventor
Elazar Rabbani
Jannis G Stavrianopoulos
James J Donegan
Dakai Liu
Norman E Kelker
Dean L Engelhardt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Therapeutics Inc
Original Assignee
Enzo Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzo Therapeutics Inc filed Critical Enzo Therapeutics Inc
Application granted granted Critical
Publication of DE69637683D1 publication Critical patent/DE69637683D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C12N15/1132—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses against retroviridae, e.g. HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/307—Circular oligonucleotides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • AIDS & HIV (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE69637683T 1995-12-15 1996-12-12 Verfahren zum Einführen von Nukleinsäuren in Zellen für therapeutische und diagnostische Verwendungen Expired - Lifetime DE69637683D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US57444395A 1995-12-15 1995-12-15

Publications (1)

Publication Number Publication Date
DE69637683D1 true DE69637683D1 (de) 2008-10-30

Family

ID=24296155

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69637683T Expired - Lifetime DE69637683D1 (de) 1995-12-15 1996-12-12 Verfahren zum Einführen von Nukleinsäuren in Zellen für therapeutische und diagnostische Verwendungen

Country Status (5)

Country Link
US (8) US20010006815A1 (de)
EP (2) EP0779365B1 (de)
JP (4) JPH09313190A (de)
CA (4) CA2279673A1 (de)
DE (1) DE69637683D1 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2279673A1 (en) * 1995-12-15 1997-06-16 Enzo Therapeutics, Inc. Property effecting and/or property exhibiting constructs for the expression of non-native nucleic acids for therapeutic and diagnostic uses
IL131979A0 (en) 1997-03-21 2001-03-19 Enzo Therapeutics Inc Vectors and viral vectors and packaging cell lines for propagating the same
US8703480B1 (en) 1997-03-21 2014-04-22 Enzo Therapeutics, Inc. Biological function effecting viral vectors and chimeric cells useful as packaging cell lines and target cells
AU9692498A (en) * 1997-10-10 1999-05-03 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Complex of biotinylated viral vector and ligand for targeted gene delivery
ES2374534T3 (es) 1998-03-20 2012-02-17 Commonwealth Scientific And Industrial Research Organisation Control de la expresión de genes.
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
JP3513068B2 (ja) * 1999-05-12 2004-03-31 浜松ホトニクス株式会社 生細胞の細胞質内標的核酸を検出する方法
EP1246532A2 (de) * 1999-06-07 2002-10-09 Mirus Corporation Zuführen von einzelstrang dna zur expression
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
US6995258B1 (en) * 2000-05-25 2006-02-07 City Of Hope Nucleolar targeting of therapeutics against HIV
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
AUPR621501A0 (en) 2001-07-06 2001-08-02 Commonwealth Scientific And Industrial Research Organisation Delivery of ds rna
US6989261B2 (en) 2001-12-20 2006-01-24 Eli Lilly And Company Butyrylcholinesterase variant polypeptides with increased catalytic efficiency and methods of use
US7049121B2 (en) 2001-12-20 2006-05-23 Applied Molecular Evolution Butyrylcholinesterase variant polypeptides with increased catalytic efficiency and methods of use
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US20060084617A1 (en) * 2002-05-06 2006-04-20 Satishchandran C Methods for delivery of nucleic acids
AU2003245648A1 (en) * 2002-06-20 2004-01-06 University Of Maryland Biotechnology Institute Scaffolded maleimide clusters for multivalent peptide assembly
US20100144038A1 (en) * 2003-03-04 2010-06-10 Masato Miyake Composition And Method For Increasing Efficiency Of Introduction Of Target Substance Into Cell
US20040265870A1 (en) * 2003-04-09 2004-12-30 Invitrogen Corporation Methods of synthesizing and labeling nucleic acid molecules
CA2568603A1 (en) * 2003-09-12 2005-03-24 Commonwealth Scientific And Industrial Research Organisation Modified gene-silencing nucleic acid molecules and uses thereof
US7951580B2 (en) * 2004-04-21 2011-05-31 The Regents Of The University Of California Automated, programmable, high throughput, multiplexed assay system for cellular and biological assays
US7695954B2 (en) * 2004-10-04 2010-04-13 The Regents Of The University Of California Micropatterned plate with micro-pallets for addressable biochemical analysis
CA2648778A1 (en) * 2006-04-10 2007-10-18 The Regents Of The University Of California Method for culturing cells on removable pallets for subsequent cell expansion and analysis
US8748085B2 (en) * 2007-07-25 2014-06-10 The Regents Of The University Of California Use of photosensitized Epon epoxy resin 1002F for MEMS and bioMEMS applications
US9487745B2 (en) 2007-07-27 2016-11-08 The Regents Of The University Of California Micro-patterned plate composed of an array of releasable elements surrounded with solid or gel walls
US20090162845A1 (en) * 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US9505709B2 (en) 2014-05-05 2016-11-29 Thetis Pharmaceuticals Llc Compositions and methods relating to ionic salts of peptides
JP7408098B2 (ja) 2017-08-18 2024-01-05 モデルナティエックス インコーポレイテッド Ｒｎａポリメラーゼバリアント
CA3097512A1 (en) * 2018-05-18 2019-11-21 F. Hoffmann-La Roche Ag Targeted intracellular delivery of large nucleic acids
CN113748129A (zh) * 2018-12-21 2021-12-03 杭州景杰生物科技股份有限公司 蛋白质赖氨酸乳酸酰化检测试剂和方法
MA55037A (fr) 2019-02-20 2021-12-29 Modernatx Inc Variants d'arn polymérase pour le coiffage co-transcriptionnel

Family Cites Families (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4847240A (en) * 1978-01-16 1989-07-11 The Trustees Of Boston University Method of effecting cellular uptake of molecules
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5241060A (en) 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US5260433A (en) 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
CA1209501A (en) 1982-09-16 1986-08-12 Nikos Panayotatos Expression vector
US4687732A (en) 1983-06-10 1987-08-18 Yale University Visualization polymers and their application to diagnostic medicine
US4707440A (en) 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US4843122A (en) 1984-01-30 1989-06-27 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US5288609A (en) 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4795706A (en) * 1985-01-31 1989-01-03 Eli Lilly And Company Novel expression control sequences
EP0273085A1 (de) 1986-12-29 1988-07-06 IntraCel Corporation Verfahren zur Einführung fremder Nukleinsäuren in eukaryotische Zellen
US5128256A (en) * 1987-01-12 1992-07-07 Stratagene DNA cloning vectors with in vivo excisable plasmids
US5811088A (en) * 1987-02-20 1998-09-22 Emory University Antiinfective compounds and methods of use
US5166320A (en) 1987-04-22 1992-11-24 University Of Connecticut Carrier system and method for the introduction of genes into mammalian cells
DE68929343T2 (de) * 1988-02-16 2002-09-12 Greatbatch Gen-Aid, Ltd. Modifizierte Zellen mit Resistenz gegen Retroviralinfektionen
US5429948A (en) * 1988-03-02 1995-07-04 Gentest Corporation Human cell line stably expressing 5cDNAS encoding procarcinogen-activating enzymes and related mutagenicity assays
US5633152A (en) 1988-06-24 1997-05-27 Carnegie Institution Of Washington Method of controlling viral growth
US5108921A (en) 1989-04-03 1992-04-28 Purdue Research Foundation Method for enhanced transmembrane transport of exogenous molecules
US5391723A (en) * 1989-05-31 1995-02-21 Neorx Corporation Oligonucleotide conjugates
US5144019A (en) * 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA
IT1230297B (it) * 1989-07-05 1991-10-18 Carmelo Lo Duca Siringa utilizzabile una sola volta.
BR9007566A (pt) 1990-05-28 1992-06-30 Univ Osaka Res Found Metodo de preparacao de plasmida,plasmida e produtos de expressao de um plasmida
US5196348A (en) * 1990-06-11 1993-03-23 Air Products And Chemicals, Inc. Perfluoro-crown ethers in fluorine magnetic resonance spectroscopy of biopsied tissue
AU652566B2 (en) * 1990-06-18 1994-09-01 United States of America, as represented by the Secretary, U.S. Department of Commerce, The Eukaryotic expression vector system
GB9016973D0 (en) 1990-08-02 1990-09-19 Medical Res Council Viral growth inhibition
US5595978A (en) 1990-08-16 1997-01-21 Isis Pharmaceuticals, Inc. Composition and method for treatment of CMV retinites
CA2072758A1 (en) 1990-09-14 1992-03-15 Kenneth Francis Buechler Antibodies to complexes of ligand receptors and ligands and their utility in ligand-receptor assays
US5171217A (en) * 1991-02-28 1992-12-15 Indiana University Foundation Method for delivery of smooth muscle cell inhibitors
JP3220180B2 (ja) * 1991-05-23 2001-10-22 三菱化学株式会社 薬剤含有タンパク質結合リポソーム
KR100235575B1 (ko) * 1991-06-27 1999-12-15 프랭크 쿵; 멜린다 그리피스, 데자딘스 캐서린 엠 Dna-결합 분자의 검출을 위한 스크리닝 분석
EP0597010A4 (en) 1991-07-31 1996-12-27 Rhone Poulenc Rorer Int Transgenic protein production.
WO1993005060A1 (de) 1991-08-28 1993-03-18 Boehringer Mannheim Gmbh Funktionalisierte trägermaterialien für gleichzeitige synthese und direktmarkierung von oligonukleotiden als primer für matrizenabhängige enzymatische nukleinsäuresynthesen
US5521291A (en) * 1991-09-30 1996-05-28 Boehringer Ingelheim International, Gmbh Conjugates for introducing nucleic acid into higher eucaryotic cells
NZ244306A (en) * 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation
US5413999A (en) * 1991-11-08 1995-05-09 Merck & Co., Inc. HIV protease inhibitors useful for the treatment of AIDS
JPH067160A (ja) * 1991-12-18 1994-01-18 New England Biolabs Inc 古細菌からの組換え熱安定性ｄｎａポリメラーゼ
US5827935A (en) 1992-05-27 1998-10-27 City Of Hope Chimeric tRNAlys -ribozyme molecules
US5587308A (en) * 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
US6503755B1 (en) 1992-10-13 2003-01-07 Armand Keating Particle transfection: rapid and efficient transfer of polynucleotide molecules into cells
US5583020A (en) * 1992-11-24 1996-12-10 Ribozyme Pharmaceuticals, Inc. Permeability enhancers for negatively charged polynucleotides
US5574142A (en) * 1992-12-15 1996-11-12 Microprobe Corporation Peptide linkers for improved oligonucleotide delivery
JPH08505872A (ja) * 1993-01-22 1996-06-25 ユニバーシティ・リサーチ・コーポレイション 治療剤の局所化
DE69434067T2 (de) * 1993-03-08 2006-03-02 Merck & Co., Inc. Menschliche neuronale nikotin-acetylcholin-rezeptor-verbindungen und methoden ihres einsatzes
WO1994020079A1 (en) * 1993-03-10 1994-09-15 Smithkline Beecham Corporation Human brain phosphodiesterase
US5359030A (en) * 1993-05-10 1994-10-25 Protein Delivery, Inc. Conjugation-stabilized polypeptide compositions, therapeutic delivery and diagnostic formulations comprising same, and method of making and using the same
US5591601A (en) 1993-05-14 1997-01-07 Ohio University Edison Animal Biotechnology Institute DNA polymerase gene expression system utilizing an RNA polymerase co-delivered with the gene expression vector system
WO1994027643A1 (en) * 1993-06-01 1994-12-08 Targeted Genetics Corporation Envelope fusion vectors for use in gene delivery
DE4318387A1 (de) * 1993-06-03 1994-12-08 Bayer Ag Rekombinante Foamy Virus Vektoren für medizinische und diagnostische Anwendungen sowie Verfahren zur Herstellung von rekombinanten Foamy Virus Vektoren
CA2162925A1 (en) * 1993-06-18 1995-01-05 Witold Filipowicz Chimeric plant genes possessing independent regulatory sequences
US5739309A (en) * 1993-07-19 1998-04-14 Gen-Probe Incorporated Enhancement of oligonucleotide inhibition of protein production, cell proliferation and / or multiplication of infectious disease pathogens
US5532130A (en) * 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
US5547862A (en) * 1993-07-29 1996-08-20 Ambion Inc. Vectors containing multiple promoters in the same orientation
GB9317380D0 (en) * 1993-08-20 1993-10-06 Therexsys Ltd Transfection process
AU7679394A (en) * 1993-09-03 1995-03-22 Duke University A method of nucleic acid sequencing
GB9319772D0 (en) 1993-09-24 1993-11-10 Therexsys Ltd Therapeutic agent
JPH0799976A (ja) * 1993-09-30 1995-04-18 Takeda Chem Ind Ltd 修飾オリゴヌクレオチド
US5614559A (en) 1993-11-23 1997-03-25 Procept Inc. Compound for inhibiting HIV infectivity
US5712384A (en) * 1994-01-05 1998-01-27 Gene Shears Pty Ltd. Ribozymes targeting retroviral packaging sequence expression constructs and recombinant retroviruses containing such constructs
US5576201A (en) * 1994-01-14 1996-11-19 Alexion Pharmaceuticals, Inc. Retroviral vector particles for transducing non-proliferating cells
US5561043A (en) * 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US5686278A (en) 1994-03-25 1997-11-11 Indiana University Foundation Methods for enhanced retrovirus-mediated gene transfer
EP0753069A1 (de) * 1994-04-15 1997-01-15 Targeted Genetics Corporation Fusionsprotein für Gentransfer
ATE200105T1 (de) * 1994-05-13 2001-04-15 Chiron Corp Verfahren und zusammensetzungen als vehikel zur zielgerichtete einbringen von genen
WO1995032225A1 (en) * 1994-05-23 1995-11-30 The Salk Institute For Biological Studies Method for site-specific integration of nucleic acids and related products
US5733781A (en) * 1994-07-19 1998-03-31 Gen-Probe Incorporated Oligonucleotides and methods for inhibiting propagation of human immunodeficiency virus
US6538107B1 (en) 1994-09-30 2003-03-25 Takeda Chemical Industries, Ltd. G protein coupled receptor protein production, and use thereof
US5734039A (en) * 1994-09-15 1998-03-31 Thomas Jefferson University Antisense oligonucleotides targeting cooperating oncogenes
CA2279673A1 (en) 1995-12-15 1997-06-16 Enzo Therapeutics, Inc. Property effecting and/or property exhibiting constructs for the expression of non-native nucleic acids for therapeutic and diagnostic uses
US5814500A (en) * 1996-10-31 1998-09-29 The Johns Hopkins University School Of Medicine Delivery construct for antisense nucleic acids and methods of use
US6077833A (en) * 1996-12-31 2000-06-20 Isis Pharmaceuticals, Inc. Oligonucleotide compositions and methods for the modulation of the expression of B7 protein
US5989814A (en) * 1997-04-01 1999-11-23 Reagents Of The University Of California Screening methods in eucaryotic cells
SK17432000A3 (sk) * 1998-05-26 2001-07-10 Icn Pharmaceuticals, Inc. Zlúčenina, ktorá zahŕňa nukleozid s 2',4'-mostíkom a oligonukleotid z nej odvodený
US6242589B1 (en) * 1998-07-14 2001-06-05 Isis Pharmaceuticals, Inc. Phosphorothioate oligonucleotides having modified internucleoside linkages
ATE490322T1 (de) * 2001-07-10 2010-12-15 Johnson & Johnson Res Pty Ltd Verfahren zur genetischen modifikation hämatopoetischer vorläuferzellen und verwendungen der modifizierten zellen

Also Published As

Publication number Publication date
US20010006816A1 (en) 2001-07-05
EP0779365A3 (de) 1999-11-24
US20030104620A1 (en) 2003-06-05
US20010007767A1 (en) 2001-07-12
CA2279675A1 (en) 1997-06-16
US8742090B2 (en) 2014-06-03
EP0779365A2 (de) 1997-06-18
EP2042606A1 (de) 2009-04-01
US20010006815A1 (en) 2001-07-05
CA2190304A1 (en) 1997-06-16
US8846893B2 (en) 2014-09-30
JP2007135605A (ja) 2007-06-07
JP2009232862A (ja) 2009-10-15
US20030087434A1 (en) 2003-05-08
US20080248564A1 (en) 2008-10-09
JP2009100782A (ja) 2009-05-14
JPH09313190A (ja) 1997-12-09
CA2279669A1 (en) 1997-06-16
US8735145B2 (en) 2014-05-27
US20010006814A1 (en) 2001-07-05
CA2279673A1 (en) 1997-06-16
US20080108139A1 (en) 2008-05-08
EP0779365B1 (de) 2008-09-17

Similar Documents

Publication Publication Date Title
DE69637683D1 (de) Verfahren zum Einführen von Nukleinsäuren in Zellen für therapeutische und diagnostische Verwendungen
DE69637393D1 (de) Vektoren und methoden zum gentransfer in zellen
DE69931085D1 (de) System und Verfahren zum Separieren von biologischen Zellen
DE69636398D1 (de) Blutbehandlungssystem und Verfahren zur Gewinnung von mononuklearen Zellen
DE69536051D1 (de) Vorrichtung und Verfahren zum In-vivo-Züchten von verschiedenen Gewebezellen
DE69533474D1 (de) Gerät und methode für die effiziente inkorporation von molekülen in zellen
KR960701078A (ko) Dna 서열화 방법과 그 장치
DE69434705D1 (de) Multiprozessorsystem und Verfahren zur Zusammensetzung von ATM Zellen
DE69631120D1 (de) Vorrichtung zum bestrahlen von lebenden zellen
DE69429773D1 (de) Zellenvermittlung und verfahren zur weglenkung von zellen durch diese vermittlung
DE69740113D1 (de) Vorrichtung und verfahren zur inkubation von zellen
DE58908694D1 (de) Oligonucleotidbank und verfahren zur dna-sequenzierung.
DE69628999D1 (de) ATP-Eliminator und Verfahren zur Bestimmung von biologischen Zellen
DE69519604D1 (de) Verfahren und Vorrichtung zum Herstellen von Tabletten
DE3582423D1 (de) Verfahren zum vernichten von zellen und zelltoetende mittel.
ATE110415T1 (de) Verbessertes verfahren und apparat, um biologische substanzen in lebenden zellen einzuführen.
DE69827019D1 (de) Gerät und verfahren zur verarbeitung von biologischen zellen
DE59813796D1 (de) Verfahren und vorrichtung zum manövrieren von kraftfahrzeugen
DE69534698D1 (de) Verfahren und Vorrichtung zum Vermittlen von Zellen
DE69515381D1 (de) Verfahren und Vorrichtung zum Herstellen von Tabletten
DE69735237D1 (de) Gerät und verfahren zum waschen von zellen
DE59809751D1 (de) Verfahren und Vorrichtung zum Herstellen von Packungen
DE69735547D1 (de) Verfahren zur induzierung von immunsupprimierten zellen und vorrichtung zu deren kultivierung
DE69605590D1 (de) Verfahren zum Nachweis von Protein und Kit dafür
DE19681321T1 (de) Radiometer und Verfahren zum Anzeigen von Wasser

Legal Events

Date Code Title Description
8364 No opposition during term of opposition