[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

ATE143694T1 - Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen - Google Patents

Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen

Info

Publication number
ATE143694T1
ATE143694T1 AT88402646T AT88402646T ATE143694T1 AT E143694 T1 ATE143694 T1 AT E143694T1 AT 88402646 T AT88402646 T AT 88402646T AT 88402646 T AT88402646 T AT 88402646T AT E143694 T1 ATE143694 T1 AT E143694T1
Authority
AT
Austria
Prior art keywords
producing
biologically active
storage protein
active peptide
protein genes
Prior art date
Application number
AT88402646T
Other languages
English (en)
Inventor
Joel Stefaan Vandekerckhove
Enno Krebbers
Johan Botterman
Jan Leemans
Original Assignee
Plant Genetic Systems Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Genetic Systems Nv filed Critical Plant Genetic Systems Nv
Application granted granted Critical
Publication of ATE143694T1 publication Critical patent/ATE143694T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT88402646T 1987-10-20 1988-10-20 Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen ATE143694T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87402348 1987-10-20

Publications (1)

Publication Number Publication Date
ATE143694T1 true ATE143694T1 (de) 1996-10-15

Family

ID=8198266

Family Applications (2)

Application Number Title Priority Date Filing Date
AT88402646T ATE143694T1 (de) 1987-10-20 1988-10-20 Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen
AT96100523T ATE316144T1 (de) 1987-10-20 1988-10-20 5'-flankierende region eines genes von arabidopsis, das für einen 2s albumin-prekursor kodiert

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT96100523T ATE316144T1 (de) 1987-10-20 1988-10-20 5'-flankierende region eines genes von arabidopsis, das für einen 2s albumin-prekursor kodiert

Country Status (4)

Country Link
US (1) US5623067A (de)
JP (1) JP3232068B2 (de)
AT (2) ATE143694T1 (de)
DE (2) DE3856589D1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6946587B1 (en) * 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US6025545A (en) * 1990-01-22 2000-02-15 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
HU220773B1 (hu) * 1990-01-22 2002-05-28 Dekalb Genetics Corporation Eljárás termő transzgenikus kukoricanövények előállítására
US6329574B1 (en) * 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
US6777589B1 (en) * 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6395966B1 (en) * 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
US6118047A (en) * 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US6281411B1 (en) 1993-08-25 2001-08-28 Dekalb Genetics Corporation Transgenic monocots plants with increased glycine-betaine content
US6326527B1 (en) * 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
US6100450A (en) * 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
WO2000012733A1 (en) * 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
US6784342B1 (en) 1999-08-04 2004-08-31 The University Of British Columbia Regulation of embryonic transcription in plants
US6403862B1 (en) 1999-09-24 2002-06-11 Pioneer Hi-Bred International, Inc. Seed-preferred promoter from maize
CA2409876C (en) * 2000-05-24 2012-01-03 The University Of British Columbia Gene regulatory region that promotes early seed-specific transcription
IL154596A0 (en) 2000-08-25 2003-09-17 Basf Plant Science Gmbh Plant polynucleotides encoding novel prenyl proteases
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
US7250296B2 (en) * 2003-03-05 2007-07-31 University Of Florida Research Foundation, Inc. Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof
JP4543236B2 (ja) 2003-03-28 2010-09-15 独立行政法人農業生物資源研究所 組換えタンパク質が高生産された植物貯蔵器官の生産方法及び新規組換えタンパク質
EP1655364A3 (de) * 2004-11-05 2006-08-02 BASF Plant Science GmbH Expressionskassetten zur bevorzugten Expression in Pflanzensamen
CA2523471A1 (en) * 2004-11-05 2006-05-05 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
EP1885862B1 (de) 2005-05-23 2015-07-29 Arcadia Biosciences Inc. Florsafran mit erhöhter gamma-linolensäure
WO2006125749A2 (en) * 2005-05-25 2006-11-30 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
WO2006136579A2 (en) * 2005-06-23 2006-12-28 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
RU2431249C2 (ru) * 2005-12-15 2011-10-20 Таргитид Гроус, Инк. Способ увеличения размера семян и числа семян в растении в результате трансгенной сверхэкспрессии гена, связанного с ростом и/или развитием, в период раннего зародышевого развития
CN101796088B (zh) 2007-09-04 2013-04-24 巴斯夫欧洲公司 生产皮革的方法、适用于此方法的共聚物及其用途
CA2867139A1 (en) 2011-04-11 2012-10-18 Targeted Growth, Inc. Identification and the use of krp mutants in plants

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE240911C (de) *
GB2068971B (en) * 1980-01-30 1983-06-08 Searle & Co Recombinant dna techniques
CA1192510A (en) * 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
WO1983001176A1 (en) * 1981-10-01 1983-04-14 Int Plant Research Inst Process for the genetic modification of cereals with transformation vectors
US4617149A (en) * 1983-09-21 1986-10-14 Eli Lilly And Company Growth hormone release factor analogs
BR8404834A (pt) * 1983-09-26 1985-08-13 Agrigenetics Res Ass Metodo para modificar geneticamente uma celula vegetal
ATE69062T1 (de) * 1985-06-12 1991-11-15 Lubrizol Genetics Inc Modifiziertes zein.
US4886878A (en) * 1985-06-12 1989-12-12 Lubrizol Genetics, Inc. Modified zein genes containing lysine
US4956282A (en) * 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
WO1987007299A1 (en) * 1986-05-29 1987-12-03 Calgene, Inc. Transformation and foreign gene expression in brassica species
NZ221259A (en) * 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
US5003045A (en) * 1986-08-29 1991-03-26 Lubrizol Genetics, Inc. Modified 7S legume seed storage proteins

Also Published As

Publication number Publication date
DE3856589D1 (de) 2006-04-06
US5623067A (en) 1997-04-22
DE3855591D1 (de) 1996-11-07
DE3855591T2 (de) 1997-04-03
JP3232068B2 (ja) 2001-11-26
ATE316144T1 (de) 2006-02-15
JP2000106890A (ja) 2000-04-18

Similar Documents

Publication Publication Date Title
ATE143694T1 (de) Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen
ATE258189T1 (de) Verfahren zur herstellung von erythropoietin frei von tierischen proteinen
DE69720026T2 (de) Verfahren zur herstellung von d-aminosäuren durch direkte gärung
ATE46536T1 (de) Verfahren zur herstellung von reifen proteinen in wirtzellen von wirbeltieren.
ATE282093T1 (de) Verfahren zur herstellung von proteinprodukten in aspergillus-oryzae und in aspergillus zu verwendender promotor
ATE271612T1 (de) Expressionskassette zur expression von beliebigen genen in pflanzensamen
DE59608743D1 (de) Verfahren zur herstellung von rekombinanten proteinen in e. coli mittels hochzelldichte-fermentation
DE69231137D1 (de) Verfahren zur Herstellung von Peroxidasen in einem Aspergillusstamm
DE3851289D1 (de) Verfahren zur Züchtung rekombinanter Zellen.
AU2912301A (en) Pyrophosphatase stress-related proteins and methods of use in plants
DE69210645D1 (de) Verfahren zur Herstellung von cysteinfreien Peptiden
DE3587422D1 (de) Fuer ein hirudinaehnliches protein kodierende dns-sequenz und verfahren zur herstellung eines solchen proteins.
ATE364703T1 (de) Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
ATE152124T1 (de) Wachstumsfaktor aus parenchymalen lebenszellen, dafür kodierendes gen, verfahren zur herstellung dieses faktors und transformanten
DE69827810D1 (de) Methode zum durchforsten von banken mittels chimären bindungspetiden
DE69738079D1 (de) Systeme zur massenherstellung von proteinen oder peptiden durch mikroorganismen der gattung humicola
ATE124454T1 (de) Gentechnische herstellung von faktor xiiia.
KR900700500A (ko) 재조합 유전자 기술에 의한 에라스타제 억제 폴리펩티드 제조방법 및 에라스타제 억제 폴리펩티드
DE69430975D1 (de) Verfahren zur Herstellung von Proteinen
ATE253639T1 (de) Verfahren zur herstellung von peptiden
KR890701606A (ko) 피치아 파스토리스로부터의 분비를 통한 동물 리소자임의 생산 및 이를 위한 조성물
ATE142263T1 (de) Verfahren zur herstellung von fremdproteinen in streptomyceten
DE69224269D1 (de) Biosynthetisches Verfahren zur Herstellung von Protein
DE68927437D1 (de) Verfahren zur herstellung von luciferase durch rekombinante expression eines luciferase-kodierenden gens
ATE116366T1 (de) Verfahren zur herstellung eines menschlichen ''neutrophil chemotactic factor''-polypeptids.