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AR111465A1 - METHOD FOR PURIFICATION OF ANALOG ANTIBODIES BY THE USE OF CATION EXCHANGE CHROMATOGRAPHY - Google Patents

METHOD FOR PURIFICATION OF ANALOG ANTIBODIES BY THE USE OF CATION EXCHANGE CHROMATOGRAPHY

Info

Publication number
AR111465A1
AR111465A1 ARP180100937A ARP180100937A AR111465A1 AR 111465 A1 AR111465 A1 AR 111465A1 AR P180100937 A ARP180100937 A AR P180100937A AR P180100937 A ARP180100937 A AR P180100937A AR 111465 A1 AR111465 A1 AR 111465A1
Authority
AR
Argentina
Prior art keywords
cation exchange
exchange chromatography
purification
antibodies
analog antibodies
Prior art date
Application number
ARP180100937A
Other languages
Spanish (es)
Inventor
Gyong Ha
- Lim Sung In Sik
Dong Eok Lee
Gyung Lee
- Lee Chung Min Hwa
Jung Min Yoo
Kyunghwa Kim
Hong Jai Lee
Sung-Muk Kang
Original Assignee
Cj Healthcare Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Healthcare Corp filed Critical Cj Healthcare Corp
Publication of AR111465A1 publication Critical patent/AR111465A1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/522—CH1 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un método para la separación de impurezas de anticuerpos análogos, por el uso de una cromatografía de intercambio de cationes, y de manera más especifica, a un método para la separación de los fragmentos individuales e isoformas de anticuerpos análogos, que se producen durante el proceso de producción de anticuerpos análogos, y la purificación de solamente fragmentos de anticuerpos de destino en alta pureza y rendimiento por el uso de un tampón de elución para la cromatografía de intercambio de cationes.A method for the separation of impurities from analogous antibodies, by the use of a cation exchange chromatography, and more specifically, to a method for the separation of individual fragments and isoforms of analogous antibodies, which occur during the process of production of analogous antibodies, and the purification of only target antibody fragments in high purity and yield by the use of an elution buffer for cation exchange chromatography.

ARP180100937A 2017-04-14 2018-04-13 METHOD FOR PURIFICATION OF ANALOG ANTIBODIES BY THE USE OF CATION EXCHANGE CHROMATOGRAPHY AR111465A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR20170048635 2017-04-14

Publications (1)

Publication Number Publication Date
AR111465A1 true AR111465A1 (en) 2019-07-17

Family

ID=63792713

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180100937A AR111465A1 (en) 2017-04-14 2018-04-13 METHOD FOR PURIFICATION OF ANALOG ANTIBODIES BY THE USE OF CATION EXCHANGE CHROMATOGRAPHY

Country Status (4)

Country Link
KR (1) KR102140693B1 (en)
AR (1) AR111465A1 (en)
TW (1) TWI679209B (en)
WO (1) WO2018190677A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102209790B1 (en) * 2018-12-20 2021-02-01 에이치케이이노엔 주식회사 Method for purification of vaccine virus using affinity chromatography
CN110066314B (en) * 2019-04-01 2023-10-27 上海药明生物技术有限公司 Affinity purification process for efficiently improving separation resolution of multimer
CN114539417A (en) * 2020-11-26 2022-05-27 盛禾(中国)生物制药有限公司 Chromatographic purification process for effectively removing bispecific antibody homodimers

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150008503A (en) * 2007-10-30 2015-01-22 제넨테크, 인크. Antibody purification by cation exchange chromatography
CN102711828B (en) * 2009-10-20 2015-06-17 Abbvie公司 Isolation and purification of anti-IL-13 antibodies using protein a affinity chromatography
CA2820095C (en) * 2010-12-21 2019-02-26 F. Hoffmann-La Roche Ag Isoform enriched antibody preparation and method for obtaining it
KR101460266B1 (en) * 2012-06-05 2014-11-11 씨제이헬스케어 주식회사 A novel method for purifying long-acting human growth hormone
KR101569783B1 (en) * 2013-06-05 2015-11-19 한화케미칼 주식회사 A Method of Antibody Purification
KR101700580B1 (en) * 2013-10-30 2017-02-13 (주)셀트리온 Method for separating antibody isoform by using cation exchange chromatography

Also Published As

Publication number Publication date
TW201841933A (en) 2018-12-01
KR102140693B1 (en) 2020-08-05
WO2018190677A3 (en) 2019-01-17
TWI679209B (en) 2019-12-11
WO2018190677A2 (en) 2018-10-18
KR20180116159A (en) 2018-10-24

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