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AR032916A1 - Dermis artificial y metodo de obtencion. - Google Patents

Dermis artificial y metodo de obtencion.

Info

Publication number
AR032916A1
AR032916A1 ARP020100722A ARP020100722A AR032916A1 AR 032916 A1 AR032916 A1 AR 032916A1 AR P020100722 A ARP020100722 A AR P020100722A AR P020100722 A ARP020100722 A AR P020100722A AR 032916 A1 AR032916 A1 AR 032916A1
Authority
AR
Argentina
Prior art keywords
artificial
skin
artificial dermis
plasma
platelet
Prior art date
Application number
ARP020100722A
Other languages
English (en)
Inventor
Noval Jose Luis Jorcano
Laguzzi Fernando Larcher
Rio Nechaevsky Marcela Del
Infiesta Alvaro Meana
Llanes Sara Gomez
Original Assignee
Ct Investig Energeticas Ciemat
Ct Comunitario De Transfusion
Fundacion Marcelino Botin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Investig Energeticas Ciemat, Ct Comunitario De Transfusion, Fundacion Marcelino Botin filed Critical Ct Investig Energeticas Ciemat
Publication of AR032916A1 publication Critical patent/AR032916A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/10—Hair or skin implants
    • A61F2/105—Skin implants, e.g. artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/33—Fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Medicinal Chemistry (AREA)
  • Dermatology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Dermis artificial obtenida a partir de plasma con plaquetas y fibroblastos humanos. El plasma con plaquetas se obtiene por fraccionamiento de la sangre total del paciente mediante centrifugacion ligera, y los fibroblastos humanos a partir de una biopsia de piel. La gelificacion se consigue mediante la adicion de calcio. Esta dermis artificial permite un crecimiento rápido de los queratinocitos sembrados en su superficie para constituir una piel artificial que es fácilmente trasplantable. Se consiguen grandes superficies de dermis artificial a partir de una pequena biopsia de piel y de cantidades mínimas de plasma con plaquetas, que al estar enriquecido en citoquinas y factores de crecimiento plaquetario potencia la proliferacion de las células sembradas, tanto en su interior como en su superficie. La piel artificial así obtenida puede servir para el tratamiento de grandes quemados, ulceras cutáneas cronicas... o utilizarse, mediante el empleo de células genéticamente modificadas, como base para terapia génica.
ARP020100722A 2001-03-01 2002-02-28 Dermis artificial y metodo de obtencion. AR032916A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200100494A ES2173812B1 (es) 2001-03-01 2001-03-01 Dermis artificial y metodo de obtencion.

Publications (1)

Publication Number Publication Date
AR032916A1 true AR032916A1 (es) 2003-12-03

Family

ID=8496947

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP020100722A AR032916A1 (es) 2001-03-01 2002-02-28 Dermis artificial y metodo de obtencion.

Country Status (14)

Country Link
US (3) US7244552B2 (es)
EP (2) EP1375647B1 (es)
JP (3) JP4235452B2 (es)
AR (1) AR032916A1 (es)
AT (1) ATE448295T1 (es)
AU (1) AU2002235958B2 (es)
CA (1) CA2439387C (es)
DE (1) DE60234323D1 (es)
DK (1) DK1375647T3 (es)
EG (1) EG23321A (es)
ES (2) ES2173812B1 (es)
PT (1) PT1375647E (es)
TW (1) TWI258506B (es)
WO (1) WO2002072800A1 (es)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7144729B2 (en) 2000-09-01 2006-12-05 Dfb Pharmaceuticals, Inc. Methods and compositions for tissue regeneration
ES2173812B1 (es) * 2001-03-01 2003-12-16 Ct Investig Energeticas Ciemat Dermis artificial y metodo de obtencion.
JP2004075661A (ja) * 2002-06-18 2004-03-11 Shiseido Co Ltd 表皮基底膜ケアを特徴とする皮膚外用剤、皮膚基底膜構造形成促進剤および人工皮膚の製造方法
GB0224986D0 (en) 2002-10-28 2002-12-04 Smith & Nephew Apparatus
US11298453B2 (en) 2003-10-28 2022-04-12 Smith & Nephew Plc Apparatus and method for wound cleansing with actives
CA2556098C (en) * 2004-02-13 2015-11-24 Intercytex Limited Wound healing composition
JP2007530016A (ja) * 2004-02-27 2007-11-01 ザ ジェネラル ホスピタル コーポレーション 発毛のための方法および組成物
US7753894B2 (en) 2004-04-27 2010-07-13 Smith & Nephew Plc Wound cleansing apparatus with stress
GB0505202D0 (en) 2005-03-14 2005-04-20 Intercytex Ltd Skin equivalent culture
ES2263382B1 (es) 2005-05-16 2007-11-16 Fundacion Para La Investigacion Biomedica Del Hospital Gregorio Marañon Matriz artificial de gel de fibrina endotelizada superproductora de factores proangiogenicos.
AU2006251004C1 (en) 2005-05-26 2013-05-23 Intercytex Limited Tissue repair using allogenic dermal fibroblasts
EP1991051A1 (en) * 2006-03-03 2008-11-19 Ciemat A mouse model comprising an engrafted human skin having hypersensitivity to uv-light
US8105380B2 (en) * 2006-10-23 2012-01-31 Stemedica Cell Technologies, Inc. Cellular scaffold
US8709081B2 (en) 2006-10-23 2014-04-29 Stemedica Cell Technologies, Inc. Cellular scaffold
US20100303770A1 (en) * 2006-12-28 2010-12-02 John Maslowski Methods for culturing dermal cells for treatment of skin injuries such as burns
JP5253749B2 (ja) * 2007-03-19 2013-07-31 肇 井上 表皮シートの作製方法および初代培養時における培養容器への上皮幹細胞の接着性付与方法
AU2008311957A1 (en) * 2007-10-15 2009-04-23 Wake Forest University Methods and compositions for printing biologically compatible nanotube composites of autologous tissue
CA2716752A1 (en) * 2008-02-29 2009-09-03 Showa University Method for producing artificial skin
CN102341130B (zh) 2009-01-27 2017-11-10 雷德德莱斯有限公司 创伤敷料、用于制造创伤敷料的方法和设备以及其储存和用途
EP2471902B9 (en) * 2009-08-25 2018-12-19 Servicio Andaluz De Salud Production of artificial tissues by means of tissue engineering using agarose-fibrin biomaterials
JP5737860B2 (ja) * 2010-04-30 2015-06-17 テルモ株式会社 ゲル状細胞組成物およびその製造方法
CL2011001870A1 (es) * 2011-08-03 2012-01-27 U Tecnica Federico Santa Maria 28 71% Proceso de preparacion de un gel de fibrina autologo o desde sangre compatible para proliferacion y vehiculizacion celular; gel de fibrina o de sangre compatible; uso del gel de fibrina o de sangre compatible para uso quirurgico.
TWI448310B (zh) * 2012-05-23 2014-08-11 Medical dressings and their coating methods for frozen freeze - dried platelets
TWI448312B (zh) * 2012-05-23 2014-08-11 An implant of freeze - dried platelets and its coating method
GB201303485D0 (en) 2013-02-27 2013-04-10 Alcyomics Ltd Skin model
MX342233B (es) * 2014-02-04 2016-08-24 Alvaro Galue Eduardo Proceso de obtencion de un compuesto de aspersion celular de fibroblastos y queratinocitos humanos en solucion y su uso como agente regenerativo de lesiones cutáneas.
ES2551143B1 (es) * 2014-05-13 2016-07-01 Fundación Tekniker Dermis artificial, piel artificial, métodos para su preparación y sus usos
CN105505855A (zh) * 2016-02-04 2016-04-20 关志广 一种具备增殖能力的自体表皮细胞apg凝胶的制作方法
CA3224181A1 (en) * 2016-05-31 2017-12-07 Octapharma Ag Plasma-based films and methods for making and using the same

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4485096A (en) * 1982-02-26 1984-11-27 Massachusetts Institute Of Technology Tissue-equivalent and method for preparation thereof
JPS6379588A (ja) * 1986-09-25 1988-04-09 Yasuo Moriya 細胞培養用基材およびその製造方法
DK475386D0 (da) * 1986-10-03 1986-10-03 Weis Fogh Ulla Sivertsen Fremgangsmaade og apparat til fremstilling af biologiske stoffer
FR2639958B1 (fr) 1988-12-06 1992-08-21 Lille Transfusion Sanguine Support biologique pour cultures cellulaires constitue de proteines plasmatiques coagulees par la thrombine, son utilisation pour la culture des keratinocytes, leur recuperation et leur transport a des fins d'utilisation therapeutique
ATE234003T1 (de) * 1992-05-29 2003-03-15 Univ North Carolina Im immobilisierten zustand getrocknete pharmazeutische verträgliche menschliche blutplättchen
JP3091113B2 (ja) * 1994-11-22 2000-09-25 科学技術振興事業団 生理活性物質分泌性のハイブリッド型ゲル
US5733545A (en) * 1995-03-03 1998-03-31 Quantic Biomedical Partners Platelet glue wound sealant
WO1997001533A1 (fr) 1995-06-27 1997-01-16 The Green Cross Corporation Derives d'acide thiocarbamique
WO2000062828A1 (en) * 1996-04-30 2000-10-26 Medtronic, Inc. Autologous fibrin sealant and method for making the same
ES2117573B1 (es) * 1996-07-19 1999-02-16 Comunitario De Transfusion Del Base dermica especialmente diseñada para el cultivo de queratinocitos.
JP3333108B2 (ja) * 1997-04-08 2002-10-07 学校法人東海大学 上皮性組織移植片およびその製造方法
ES2132027B1 (es) * 1997-07-04 2000-04-01 Comunitario De Transfusion Del Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante.
JPH11239609A (ja) * 1998-02-26 1999-09-07 Sekisui Chem Co Ltd 血小板含有生体組織用接着剤
JPH11246420A (ja) * 1998-02-26 1999-09-14 Sekisui Chem Co Ltd 創傷治癒促進剤
ES2173812B1 (es) * 2001-03-01 2003-12-16 Ct Investig Energeticas Ciemat Dermis artificial y metodo de obtencion.
ES2184623B1 (es) * 2001-06-29 2004-09-16 Centro De Investigaciones Energeticas, Medioambientales Y Tecnologicas (C.I.E.M.A.T.) Piel artificial autologa secretora de leptina y metodo de obtencion.
AU2002343738A1 (en) * 2001-11-15 2003-05-26 University Of Medicine And Dentistry Of New Jersey A three-dimensional matrix for producing living tissue equivalents
US20040126881A1 (en) * 2002-09-06 2004-07-01 Vincent Ronfard Fibrin cell supports and methods of use thereof

Also Published As

Publication number Publication date
JP4235452B2 (ja) 2009-03-11
JP5047047B2 (ja) 2012-10-10
JP2004522545A (ja) 2004-07-29
DK1375647T3 (da) 2010-03-15
JP5529918B2 (ja) 2014-06-25
US7244552B2 (en) 2007-07-17
US20070275461A1 (en) 2007-11-29
DE60234323D1 (de) 2009-12-24
US20120183505A1 (en) 2012-07-19
EP1375647A1 (en) 2004-01-02
WO2002072800A1 (es) 2002-09-19
EP1375647B1 (en) 2009-11-11
PT1375647E (pt) 2010-02-18
ATE448295T1 (de) 2009-11-15
ES2173812A1 (es) 2002-10-16
CA2439387A1 (en) 2002-09-19
TWI258506B (en) 2006-07-21
EP2165678A1 (en) 2010-03-24
ES2336430T3 (es) 2010-04-13
JP2012187412A (ja) 2012-10-04
AU2002235958B2 (en) 2007-05-10
US20040171145A1 (en) 2004-09-02
JP2008253788A (ja) 2008-10-23
EG23321A (en) 2004-12-29
ES2173812B1 (es) 2003-12-16
US9168125B2 (en) 2015-10-27
CA2439387C (en) 2012-04-24

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