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NO914886L - Chymosinutvinning og -rensing - Google Patents

Chymosinutvinning og -rensing

Info

Publication number
NO914886L
NO914886L NO91914886A NO914886A NO914886L NO 914886 L NO914886 L NO 914886L NO 91914886 A NO91914886 A NO 91914886A NO 914886 A NO914886 A NO 914886A NO 914886 L NO914886 L NO 914886L
Authority
NO
Norway
Prior art keywords
filtrate
chymosin
phenyl
recovery
sepharose resin
Prior art date
Application number
NO91914886A
Other languages
English (en)
Other versions
NO914886D0 (no
Inventor
Henry G Heinsohn
Matthew B Murphy
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of NO914886D0 publication Critical patent/NO914886D0/no
Publication of NO914886L publication Critical patent/NO914886L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Denne oppfinnelse frembringer en fremgangsmåte ved , utvinnelse og rensing av chymosin fra fermentasjon anvendt for mikrobiologisk produksjon av chymosin. Fermentasjonsblandingen filtreres for å gi et filtrat som inneholder chymosinet. Filtratet prepareres fcrst ved enten å innstille det til en lav pH-verdi, såsom ca. ; 2, og/eller innstilles saltkonsentrasjonen i filtratet ; til minst ca. 2M. Den vandige filtratblanding bringes i ; kontakt med en fenyl-sefarose-harpiks som selektivt binder chymosinet fra filtratet. Filtratet som er igjen etter kontakten med fenyl-sefarose-harpiksen kastes, og : fenyl-sefarose-harpikskolonnen elueres med vann eller i fortynnet saltopplcsning, slik at man oppnår i alt j vesentlig rent chymosin i et eneste elueringstrinn.
NO91914886A 1989-06-13 1991-12-12 Chymosinutvinning og -rensing NO914886L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36594489A 1989-06-13 1989-06-13
PCT/US1990/003377 WO1990015865A1 (en) 1989-06-13 1990-06-13 Chymosin recovery and purification

Publications (2)

Publication Number Publication Date
NO914886D0 NO914886D0 (no) 1991-12-12
NO914886L true NO914886L (no) 1991-12-12

Family

ID=23441043

Family Applications (1)

Application Number Title Priority Date Filing Date
NO91914886A NO914886L (no) 1989-06-13 1991-12-12 Chymosinutvinning og -rensing

Country Status (10)

Country Link
EP (1) EP0477277B1 (no)
JP (1) JP2974763B2 (no)
AU (1) AU629656B2 (no)
CA (1) CA2058453C (no)
DE (1) DE69018823T2 (no)
DK (1) DK0477277T3 (no)
FI (1) FI100110B (no)
NO (1) NO914886L (no)
NZ (1) NZ234058A (no)
WO (1) WO1990015865A1 (no)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
DK1257562T3 (da) * 2000-02-08 2007-04-02 Upfront Chromatography As Hidtil ukendte harpiksmaterialer og anvendelsen deraf til isolering af proteiner eller peptider
WO2007118838A1 (en) 2006-04-13 2007-10-25 Dsm Ip Assets B.V. Liquid composition comprising an aspartic protease
WO2012127005A1 (en) 2011-03-22 2012-09-27 Dsm Ip Assets B.V. Chymosin formulation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI841288A (fi) * 1983-03-31 1984-10-01 Codon Genetic Eng Lab Rekombinant-dna-kod foer uttryckning av polypeptid.
US4743551A (en) * 1984-11-05 1988-05-10 Miles Inc. Purification of microbial rennet from Mucor miehei
US4745063A (en) * 1986-10-01 1988-05-17 Sanofi Bio Ingredients, Inc. Method for separating rennet components
CA2058948C (en) * 1989-06-13 1995-05-02 Henry G. Heinsohn Processes for the recovery of naturally produced chymosin

Also Published As

Publication number Publication date
WO1990015865A1 (en) 1990-12-27
NO914886D0 (no) 1991-12-12
EP0477277A4 (en) 1992-05-13
JPH05500301A (ja) 1993-01-28
CA2058453A1 (en) 1990-12-14
AU629656B2 (en) 1992-10-08
JP2974763B2 (ja) 1999-11-10
DK0477277T3 (da) 1995-08-21
FI100110B (fi) 1997-09-30
DE69018823T2 (de) 1995-12-07
NZ234058A (en) 1992-07-28
AU5852290A (en) 1991-01-08
EP0477277A1 (en) 1992-04-01
EP0477277B1 (en) 1995-04-19
DE69018823D1 (de) 1995-05-24
CA2058453C (en) 1999-06-01
FI915812A0 (fi) 1991-12-10

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