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MX2023008629A - Nuevas nucleasas modificadas y quimericas. - Google Patents

Nuevas nucleasas modificadas y quimericas.

Info

Publication number
MX2023008629A
MX2023008629A MX2023008629A MX2023008629A MX2023008629A MX 2023008629 A MX2023008629 A MX 2023008629A MX 2023008629 A MX2023008629 A MX 2023008629A MX 2023008629 A MX2023008629 A MX 2023008629A MX 2023008629 A MX2023008629 A MX 2023008629A
Authority
MX
Mexico
Prior art keywords
nucleases
engineered
chimeric
chimeric nucleases
novel engineered
Prior art date
Application number
MX2023008629A
Other languages
English (en)
Inventor
Christopher Brown
Cristina Butterfield
Greg Cost
Rebecca Lamothe
Jyun-Liang Lin
Brian C Thomas
Morayma M Temoche-Diaz
Alan Brooks
Original Assignee
Metagenomi Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metagenomi Inc filed Critical Metagenomi Inc
Publication of MX2023008629A publication Critical patent/MX2023008629A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03015—(S)-2-Hydroxy-acid oxidase (1.1.3.15)
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En la presente descripción se describen nucleasas modificadas genéticamente y sistemas de nucleasas, que incluyen nucleasas quiméricas y sistemas de nucleasas quiméricas. Las nucleasas quiméricas y modificadas genéticamente descritas en la presente descripción incluyen nucleasa guiada por ácido nucleico. Adicionalmente, en la presente descripción se describen métodos para generar nucleasas modificadas genéticamente y métodos para usarlas.
MX2023008629A 2021-01-22 2022-01-21 Nuevas nucleasas modificadas y quimericas. MX2023008629A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163140620P 2021-01-22 2021-01-22
US202163237484P 2021-08-26 2021-08-26
PCT/US2022/013396 WO2022159758A1 (en) 2021-01-22 2022-01-21 Novel engineered and chimeric nucleases

Publications (1)

Publication Number Publication Date
MX2023008629A true MX2023008629A (es) 2023-10-25

Family

ID=82549895

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023008629A MX2023008629A (es) 2021-01-22 2022-01-21 Nuevas nucleasas modificadas y quimericas.

Country Status (10)

Country Link
US (1) US20230416710A1 (es)
EP (1) EP4281554A1 (es)
JP (1) JP2024504981A (es)
KR (1) KR20230134543A (es)
AU (1) AU2022210762A1 (es)
BR (1) BR112023014719A2 (es)
CA (1) CA3205865A1 (es)
GB (1) GB2610711B (es)
MX (1) MX2023008629A (es)
WO (1) WO2022159758A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022011039A (es) 2020-03-06 2022-12-13 Metagenomi Inc Sistemas crispr clase ii tipo v.
MX2024002539A (es) * 2021-08-27 2024-03-19 Metagenomi Inc Enzimas con dominios ruvc.
WO2024026499A2 (en) * 2022-07-29 2024-02-01 Metagenomi, Inc. Class ii, type v crispr systems
WO2024156085A1 (en) * 2023-01-27 2024-08-02 Syngenta Crop Protection Ag Mb2cas12a variants with enhanced efficiency

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2757325T3 (es) * 2012-12-06 2020-04-28 Sigma Aldrich Co Llc Modificación y regulación del genoma en base a CRISPR
US9963689B2 (en) * 2013-12-31 2018-05-08 The Regents Of The University Of California Cas9 crystals and methods of use thereof
WO2019051278A1 (en) * 2017-09-07 2019-03-14 The Board Of Trustees Of The Leland Stanford Junior University NUCLEASE SYSTEMS FOR GENETIC ENGINEERING
CA3117228A1 (en) * 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
CN116515797A (zh) * 2019-02-14 2023-08-01 宏基因组学公司 具有ruvc结构域的酶
US10982200B2 (en) * 2019-02-14 2021-04-20 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
KR20230021657A (ko) * 2020-05-08 2023-02-14 메타지노미, 인크. Ruvc 도메인을 포함하는 효소
KR20230062873A (ko) * 2020-09-11 2023-05-09 메타지노미, 인크. 염기 편집 효소
AU2022237663A1 (en) * 2021-03-19 2023-10-12 Metagenomi, Inc. Multiplex editing with cas enzymes

Also Published As

Publication number Publication date
CA3205865A1 (en) 2022-07-28
GB2610711A (en) 2023-03-15
AU2022210762A1 (en) 2023-08-24
KR20230134543A (ko) 2023-09-21
WO2022159758A1 (en) 2022-07-28
GB2610711B (en) 2023-08-02
GB202215621D0 (en) 2022-12-07
EP4281554A1 (en) 2023-11-29
JP2024504981A (ja) 2024-02-02
AU2022210762A9 (en) 2024-09-19
BR112023014719A2 (pt) 2023-12-05
US20230416710A1 (en) 2023-12-28

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