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MX2020003497A - Metodo para generar anticuerpos multiespecificos a partir de anticuerpos monoespecificos. - Google Patents

Metodo para generar anticuerpos multiespecificos a partir de anticuerpos monoespecificos.

Info

Publication number
MX2020003497A
MX2020003497A MX2020003497A MX2020003497A MX2020003497A MX 2020003497 A MX2020003497 A MX 2020003497A MX 2020003497 A MX2020003497 A MX 2020003497A MX 2020003497 A MX2020003497 A MX 2020003497A MX 2020003497 A MX2020003497 A MX 2020003497A
Authority
MX
Mexico
Prior art keywords
antibodies
monospecific
generating multispecific
generating
multispecific antibodies
Prior art date
Application number
MX2020003497A
Other languages
English (en)
Inventor
Guy Georges
Ulrich Brinkmann
Eike Hoffmann
Stefan Dengl
Klaus Mayer
Felix Bormann
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2020003497A publication Critical patent/MX2020003497A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/522—CH1 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/524—CH2 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/53—Hinge
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/66—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a swap of domains, e.g. CH3-CH2, VH-CL or VL-CH1

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

En la presente se reporta un método para la generación de anticuerpos multiespecíficos por una reacción de intercambio de mitades de anticuerpos entre dos 2/3-IgG desestabilizadas por la mitad mediante mutaciones perturbadoras asimétricas que fomentan la generación de anticuerpos biespecíficos de longitud completa correctamente ensamblados. El método se puede realizar en la ausencia de agentes reductores y no requiere enlaces de disulfuro de región bisagra en las 2/3-IgG iniciales.
MX2020003497A 2017-10-20 2018-10-19 Metodo para generar anticuerpos multiespecificos a partir de anticuerpos monoespecificos. MX2020003497A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17197616 2017-10-20
PCT/EP2018/078675 WO2019077092A1 (en) 2017-10-20 2018-10-19 METHOD FOR GENERATING MULTISPECIFIC ANTIBODIES FROM MONOSPECIFIC ANTIBODIES

Publications (1)

Publication Number Publication Date
MX2020003497A true MX2020003497A (es) 2020-07-22

Family

ID=60268188

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020003497A MX2020003497A (es) 2017-10-20 2018-10-19 Metodo para generar anticuerpos multiespecificos a partir de anticuerpos monoespecificos.

Country Status (12)

Country Link
US (1) US20200392253A1 (es)
EP (1) EP3697441B1 (es)
JP (1) JP7450535B2 (es)
KR (1) KR20200067197A (es)
CN (1) CN111246885B (es)
AU (1) AU2018353420A1 (es)
BR (1) BR112020007154A2 (es)
CA (1) CA3078157A1 (es)
IL (1) IL273849A (es)
MX (1) MX2020003497A (es)
TW (1) TW201932142A (es)
WO (1) WO2019077092A1 (es)

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Also Published As

Publication number Publication date
AU2018353420A1 (en) 2020-04-16
JP7450535B2 (ja) 2024-03-15
TW201932142A (zh) 2019-08-16
JP2021500348A (ja) 2021-01-07
EP3697441B1 (en) 2023-06-07
US20200392253A1 (en) 2020-12-17
EP3697441A1 (en) 2020-08-26
BR112020007154A2 (pt) 2020-09-29
CA3078157A1 (en) 2019-04-25
KR20200067197A (ko) 2020-06-11
WO2019077092A1 (en) 2019-04-25
CN111246885A (zh) 2020-06-05
CN111246885B (zh) 2024-06-11
IL273849A (en) 2020-05-31

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