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Lilis et al., 2024 - Google Patents

Optimized Calcium-Phosphate-Based Co-transfection of Tau and tdTomato into Human iPSC-Derived Neurons for the Study of Intracellular Distribution of Wild-type …

Lilis et al., 2024

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Document ID
10749813779828163687
Author
Lilis P
Al Kabbani M
Zempel H
Publication year
Publication venue
Tau Protein: Methods and Protocols

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Snippet

The study of Tau protein in disease-relevant neuronal cells in culture requires efficient delivery systems for transfection of exogenous Tau and also modulators and interactors of Tau. Transfection of cultivated cells using calcium phosphate precipitation is a simple and …
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2502/30—Coculture with; Conditioned medium produced by tumour cells

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