[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

Lago et al., 2023 - Google Patents

Medulloblastoma and high-grade glioma organoids for drug screening, lineage tracing, co-culture and in vivo assay

Lago et al., 2023

View HTML
Document ID
16216506427713884052
Author
Lago C
Gianesello M
Santomaso L
Leva G
Ballabio C
Anderle M
Antonica F
Tiberi L
Publication year
Publication venue
Nature Protocols

External Links

Snippet

Medulloblastoma and high-grade glioma represent the most aggressive and frequent lethal solid tumors affecting individuals during pediatric age. During the past years, several models have been established for studying these types of cancers. Human organoids have recently …
Continue reading at communities.springernature.com (HTML) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics

Similar Documents

Publication Publication Date Title
Rouhi et al. Hypoxia-induced metastasis model in embryonic zebrafish
Miura et al. Engineering brain assembloids to interrogate human neural circuits
Giandomenico et al. Generation and long-term culture of advanced cerebral organoids for studying later stages of neural development
Lago et al. Medulloblastoma and high-grade glioma organoids for drug screening, lineage tracing, co-culture and in vivo assay
Watanabe et al. Transplantation of intestinal organoids into a mouse model of colitis
Beaudoin III et al. Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex
Crouch et al. FACS isolation of endothelial cells and pericytes from mouse brain microregions
Marciniak et al. Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology
Ferrón et al. A combined ex/in vivo assay to detect effects of exogenously added factors in neural stem cells
Smith et al. An in-vitro preparation of isolated enteric neurons and glia from the myenteric plexus of the adult mouse
Kang et al. Embryonic and induced pluripotent stem cell staining and sorting with the live-cell fluorescence imaging probe CDy1
Van Dycke et al. Infection of zebrafish larvae with human norovirus and evaluation of the in vivo efficacy of small-molecule inhibitors
Lin et al. Monitoring cancer cell invasion and T-cell cytotoxicity in 3D culture
Sarrafha et al. High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells
Sassen et al. Embryonic zebrafish primary cell culture for transfection and live cellular and subcellular imaging
Helsel et al. Transplantation as a quantitative assay to study mammalian male germline stem cells
Ortega et al. Live imaging of adult neural stem cells in rodents
Groenewoud et al. Ortho-and ectopic zebrafish xeno-engraftment of ocular melanoma to recapitulate primary tumor and experimental metastasis development
Gabriel et al. Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia
Thrikawala et al. Infection of zebrafish larvae with aspergillus spores for analysis of host-pathogen interactions
Wolfes et al. Culturing in vivo-like murine astrocytes using the fast, simple, and inexpensive AWESAM protocol
Klenke et al. Culturing embryonic nasal explants for developmental and physiological study
Nichols et al. Ex utero electroporation and whole hemisphere explants: a simple experimental method for studies of early cortical development
Sherafat et al. Organotypic slice cultures to study oligodendrocyte proliferation, fate, and myelination
Núñez et al. Evaluation of biomarkers in glioma by immunohistochemistry on paraffin-embedded 3D glioma neurosphere cultures