Quantification of Local Morphodynamics and Local GTPase Activity by Edge Evolution Tracking
Figure 8
Comparison of morphodynamic analysis by EET with polar coordinate-based analysis.
(A) Polar coordinate-based analysis was performed by setting the origin of coordinates at the mean mass center of the binary images. (B) Time-shifted cross-correlation analysis by polar coordinates and EET for the cell depicted in Figure 5. Both of the correlation profiles show positive correlations with negative time-shifts and low correlations with positive time-shifts. However, EET yields a higher correlation than the polar coordinate-based method for the negative time-shifts. (C) The same cells in Figure 7 were also analyzed by polar coordinate-based analysis. All panels show similar shapes to that in Figure 7; however, peaks in Cdc42 and Rac were lower with polar coordinate-based analysis than with EET.