In vitro evaluation of genomic damage induced by glyphosate on human lymphocytes

• Alvarez-Moya C, Silva MR, Ramírez CV, Gallardo DG, Sanchez RL, Aguirre AC, Velasco AF (2014) Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms. Genet Mol Biol 37:105–110

  Article  Google Scholar 

• Aris A, Leblanc S (2011) Maternal and fetal exposure to pesticides associated to genetically modified foods in eastern townships of Quebec, Canada. Rep Toxicol 31:528–533

  Article  CAS  Google Scholar 

• Bai SH, Ogbourne SM (2016) Glyphosate: environmental contamination, toxicity and potential risks to human health via food contamination. Environ Sci Pollut Res 23:18988–19001

  Article  CAS  Google Scholar 

• Bonassi S, Znaor A, Norppa H, Hagmar L (2004) Chromosomal aberrations and risk of cancer in humans: an epidemiologic perspective. Cytogenet Genome Res 104:376–382

  Article  CAS  Google Scholar 

• Bonassi S, El-Zein R, Bolognesi C (2011) Micronuclei frequency in peripheral blood lymphocytes and cancer risk: evidence from human studies. Mutagenesis 26:93–100

  Article  CAS  Google Scholar 

• Braz-Mota S, Sadauskas-Henrique H, Duarte RM, Val AL, Almeida-Val VM (2015) Roundup® exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macropomum. Chemosphere 135:53–60

  Article  CAS  Google Scholar 

• Duke SO (2017) The history and current status of glyphosate. Pest Manag Sci 74:1027–1034. https://doi.org/10.1002/ps.4652

  Article  CAS  Google Scholar 

• European Commission (2017) Health and Food Safety Directorate-General. Summary report of the Appeal Committee – Phytopharmaceuticals – Plant protection Products – Legislation. https://ec.europa.eu/food/sites/food/files/plant/docs/sc_phyto_20171127_pppl_summary.pdf. Accessed 02-29-2018

• European Food Safety Authority (EFSA) (2014) The 2011 European Union report on pesticide residues in food. EFSA J 12:3694 Available from: http://onlinelibrary.wiley.com/doi/10.2903/j.efsa.2014.3694/epdf. Accessed on 03-09-2018

  Google Scholar 

• European Food Safety Authority (EFSA) (2015) Conclusion on the peer review of the pesticide risk assessment of the active substance glyphosate (EFSA-Q-2014-00546, EFSA-Q-2015-00279, approved on 30 October 2015 by European Food Safety Authority). EFSA J 13(11):4302 Available from: http://onlinelibrary.wiley.com/doi/10.2903/j.efsa.2015.4302/epdf. Accessed on 03-09-2018

  Google Scholar 

• FAO/WHO Joint Meeting on Pesticide Residues (JMPR) (2014) Glyphosate. In: Pesticide residues in food 2013. Joint FAO/WHO meeting on pesticide residues and the WHO core assessment group on pesticide residues, Geneva, 17–26 September 2013. Rome: Food and Agriculture Organization of the United Nations/Geneva, World Health Organization (WHO). (FAO Plant Production and Protection Paper No. 219); pp 225–228. Available from: http://www.fao.org/3/a-i3518e.pdf. Accessed on 03-08-2018

• FAO/WHO Joint Meeting on Pesticide Residues (JMPR) (2016) Summary report for diazinon, glyphosate, malathion. Geneva, Switzerland: food and agriculture organization of the United Nations/Geneva, World Health Organization (WHO). pp 2. Available from: http://www.who.int/foodsafety/jmprsummary2016.pdf?ua=1. Accesse on 03-08-2018

• Ferrer E, Santoni E, Vittori S, Font G, Mañes J, Sagratini G (2011) Simultaneous determination of bisphenol a, octylphenol, and nonylphenol by pressurised liquid extraction and liquid chromatography–tandem mass spectrometry in powdered milk and infant formulas. Food Chem 126:360–367

  Article  CAS  Google Scholar 

• George J, Prasad S, Mahmood Z, Shukla Y (2010) Studies on glyphosate-induced carcinogenicity in mouse skin: a proteomic approach. J Proteome 73:951–964

  Article  CAS  Google Scholar 

• Hoppe HW, Rüther M, Pieper S, Kolossa-Gehring M (2017) Glyphosate in German adults—time trend (2001 to 2015) of human exposure to a widely used herbicide. Int J Hyg Environ Health 220:8–16

  Article  Google Scholar 

• International Agency for Research on Cancer (IARC) Working Group (2015) Glyphosate. In: Some Organophosphate Insecticides and Herbicides: Diazinon, Glyphosate, Malathion, Parathion, and Tetrachlorvinphos. IARC Monogr 112:321–399 Available from: http://monographs.iarc.fr/ENG/Monographs/vol112/mono112.pdf. Accessed on 03-08-2018

  Google Scholar 

• Kašuba V, Milić M, Rozgaj R, Kopjar N, Mladinić M, Žunec S, Vrdoljak AL, Pavičić I, Marjanović Čermak AM, Pizent A, Lovaković BT, Želježić D (2017) Effects of low doses of glyphosate on DNA damage, cell proliferation and oxidative stress in the HepG2 cell line. Environ Sci Pollut Res 24:19267–19281

  Article  Google Scholar 

• Kier LD, Kirkland DJ (2013) Review of genotoxicity studies of glyphosate and glyphosate-based formulations. Crit Rev Toxicol 43:283–315

  Article  CAS  Google Scholar 

• King JJ, Wagner RS (2010) Toxic effects of the herbicide roundup® regular on Pacific northwestern amphibians. Northwest Nat 91:318–324

  Article  Google Scholar 

• Kocaman AY, Rencüzoğullari E, Topaktaş M (2014) In vitro investigation of the genotoxic and cytotoxic effects of thiacloprid in cultured human peripheral blood lymphocytes. Environ Toxicol 29:631–641

  Article  CAS  Google Scholar 

• Koller V, Fürhacker M, Nersesyan A, Mišík M, Eisenbauer M, Knasmueller S (2012) Cytotoxic and DNA-damaging properties of glyphosate and roundup in human-derived buccal epithelial cells. Arch Toxicol 86:805–813

  Article  CAS  Google Scholar 

• Kwiatkowska M, Jarosiewicz P, Michałowicz J, Koter-Michalak M, Huras B, Bukowska B (2016) The impact of glyphosate, its metabolites and impurities on viability, ATP level and morphological changes in human peripheral blood mononuclear cells. PLoS One 11:e0156946

  Article  Google Scholar 

• Kwiatkowska M, Reszka E, Woźniak K, Jabłońska E, Michałowicz J, Bukowska B (2017) DNA damage and methylation induced by glyphosate in human peripheral blood mononuclear cells (in vitro study). Food Chem Toxicol 105:93–98

  Article  CAS  Google Scholar 

• Lioi MB, Scarfi MR, Santoro A, Barbieri R, Zeni O, Di Berardino D, Ursini MV (1998) Genotoxicity and oxidative stress induced by pesticide exposure in bovine lymphocytes cultures in vitro. Mutat Res 403:13–20

  Article  CAS  Google Scholar 

• Majewski MS, Coupe RH, Foreman WT, Capel PD (2014) Pesticides in Mississippi air and rain: a comparison between 1995 and 2007. Environ Toxicol Chem 33:1283–1293

  Article  CAS  Google Scholar 

• Mañas F, Peralta L, Raviolo J, Ovando HG, Weyers A, Ugnia L, Cid MG, Larripa I, Gorla N (2009) Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests. Environ Toxicol Pharmacol 28:37–41

  Article  Google Scholar 

• Marc J, Mulner-Lorillon O, Bellé R (2004) Glyphosate-based pesticides affect cell cycle regulation. Biol Cell 96:245–249

  Article  CAS  Google Scholar 

• Marques A, Guilherme S, Gaivão I, Santos MA, Pacheco M (2014) Progression of DNA damage induced by a glyphosate-based herbicide in fish (Anguilla anguilla) upon exposure and post-exposure periods--insights into the mechanisms of genotoxicity and DNA repair. Comp Biochem Physiol C Toxicol Pharmacol 166:126–133

  Article  CAS  Google Scholar 

• Mladinic M, Berend S, Vrdoljak AL, Kopjar N, Radic B, Zeljezic D (2009) Evaluation of genome damage and its relation to oxidative stress induced by glyphosate in human lymphocytes in vitro. Environ Mol Mutagen 50:800–807

  Article  CAS  Google Scholar 

• Piesova E (2005) The effect of glyphosate on the frequency of micronuclei in bovine lymphocytes in vitro. Acta Veter 55:101–109

  Article  Google Scholar 

• Romano MA, Romano RM, Santos LD, Wisniewski P, Campos DA, de Souza PB, Viau P, Bernardi MM, Nunes MT, de Oliveira CA (2012) Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression. Arch Toxicol 86:663–673

  Article  CAS  Google Scholar 

• Santovito A, Cannarsa E, Schleicherova D, Cervella P (2018) Clastogenic effects of bisphenol a on human cultured lymphocytes. Hum Exp Toxicol 37:69–77

  Article  CAS  Google Scholar 

• Šiviková K, Dianovský J (2006) Cytogenetic effect of technical glyphosate on cultivated bovine peripheral lymphocytes. Int J Hyg Environ Health 209:15–20

  Article  Google Scholar 

• Thongprakaisang S, Thiantanawat A, Rangkadilok N, Suriyom T, Satayavivad J (2013) Glyphosate induces human breast cancer cells growth via estrogen receptors. Food Chem Toxicol 59:129–136

  Article  CAS  Google Scholar 

• United States Environmental Protection Agency (US EPA) (2012) Glyphosate. Section 3 registration concerning the application of glyphosate to carrots, sweet potato, teff, oilseeds (crop group (CG) 20) and to update the CG definitions for bulb vegetable (CG 3–07), fruiting vegetable (CG 8–10), citrus fruit (CG 10–10), porne fruit (CG 11–10), berry (CG 13–07), human health risk assessment. Washington (DC): U.S. Environmental Protection Agency (US EPA), Office of Chemical Safety and Pollution Prevention (No. Decision No.: 459870); pp 28

• Yüzbaşioğlu D, Celik M, Yilmaz S, Unal F, Aksoy H (2006) Clastogenicity of the fungicide afugan in cultured human lymphocytes. Mutat Res 604:53–59

  Article  Google Scholar 

• Zouaoui K, Dulaurent S, Gaulier JM, Moesch C, Lachatre G (2013) Determination of glyphosate and AMPA in blood and urine from humans: about cases of acute intoxication. Forensic Sci Int 226:20–25

  Article  Google Scholar 

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