Abstract
Chinese hamster ovary (CHO) cells can produce proteins with complex structures and post-translational modifications which are similar to human-derived cells, and they have been the ideal host cells for the production of recombinant therapy proteins (RTPs). Nearly 70% of approved RTPs are produced by CHO cells. In recent years, a series of measures have been developed to increase the expression of RTPs to achieve the lower production cost during the process of large-scale industrial production of recombinant protein in CHO cells. Among of them, the addition of small molecule additives in the culture medium can improve the expression and production efficiency of recombinant proteins, and has become an effective and simple method. In this paper, the characteristics of CHO cells, the effect and mechanism of small molecule additives are reviewed.
Key points
• Small molecular additives on the expression of RTPs in CHO cells are reviewed
• Small molecular additives improve the yield of RTPs
• Small molecular additives provide methods for the optimization of serum-free medium
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This work was funded by the Scientific and Technological Brainstorm Project of Henan Province (No. 212102310318) and Science and Technology Innovation Leading Talent Project of Central Plains, China (234200510003).
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Q.Z. wrote and edited the manuscript. C.M. investigated and edited the manuscript. T.W. designed the content of the review and funded this manuscript.
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Zhang, Q., Mi, C. & Wang, T. Effects and mechanism of small molecule additives on recombinant protein in CHO cells. Appl Microbiol Biotechnol 107, 2771–2781 (2023). https://doi.org/10.1007/s00253-023-12486-4
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DOI: https://doi.org/10.1007/s00253-023-12486-4