Search Results (117,673)

Abstract: Background: multilocular thymic cysts are uncommon acquired cysts in the anterior mediastinum caused by incomplete thymic involution. They may be associated with autoimmune diseases, such as rheumatoid arthritis and systemic sclerosis. Methods: a 61-year-old man with a history of rheumatoid arthritis for 8 years was referred to our unit because of a multiloculated mass in the anterior mediastinum with a high F¹⁸ fluorodeoxyglucose uptake at PET-CT scan. Histology showed a multilocular thymic cyst with lymphoid tissue, organized in germinal centers and internodal areas. Results: rheumatoid arthritis-related symptoms progressively disappeared after the excision of the mass. Conclusion: to our knowledge, this is the first report in the English language of rheumatoid arthritis symptoms improvement after multilocular thymic cyst surgical removal. Full article

Knowing the energy balance in agricultural systems is essential for a holistic understanding of sustainability, productivity and economic return. The aim of this study was to estimate the cumulative energy demand (CED), greenhouse gas (GHG) emissions and carbon footprint in industrial potato and tomato production systems in the Southeast region of Brazil, identifying mitigation strategies in different scenarios. The Life Cycle Analysis methodology was used, and two functional units were defined: one hectare of cultivation and one kilogram of vegetable produced. The CEDs for tomato and potato production were 59,553.56 MJ ha^–1 (or 0.54 MJ kg^–1) and 57,992.02 MJ ha^–1 (or 1.45 MJ kg^–1), respectively. The GHG emissions were 5425.13 kg CO₂ eq ha^–1 for potato production and 5270.9 kg CO₂ eq ha^–1 for tomato production, resulting in carbon footprints of 0.135 and 0.042 kg CO₂ eq kg^–1, respectively. Fertilizers, diesel and pesticides were the main contributors to CED and GHG emissions. Thus, in order to achieve greater sustainability in the production of these vegetables and mitigate the impacts on the environment generated by the high demand for energy and GHG emissions, it is necessary to replace synthetic fertilizers with organic sources, chemical pesticides with biological pesticides, diesel with biodiesel or the use of electric vehicles and tractors, resulting in reductions of up to 39 and 52% in the GHG emissions for potatoes and tomatoes, respectively. Full article

Background/Objective: Chlamydia abortus, the cause of ovine enzootic abortion, is a zoonotic bacterial pathogen and one of the most infectious causes of foetal death in sheep worldwide. Although the disease can be controlled using commercial inactivated and live whole-organism vaccines, there are issues with both, particularly concerning efficacy and safety. Recently, we have described the development of a new COMC (chlamydial outer membrane complex) vaccine based on a detergent-extracted outer membrane protein preparation of the pathogen, which can be delivered in a single inoculation and is both efficacious and safe. Methods: In this study, we have evaluated the COMC vaccine further in a dose–response titration of the chlamydial antigen content of the vaccine (from 20 to 2.5 µg in seven experimental groups) using an established pregnant sheep challenge model. Results: No obvious dose–response relationship was observed across the groups, with a single abortion event occurring in four of the groups and three in the lowest dose group (2.5 µg). No abortions occurred in the 15 and 10 µg groups. The abortion rates (0–14%) were significantly below that of the challenge control group (33%). A similar reduction in bacterial shedding of infectious organisms following parturition was observed in the vaccinated groups compared to the challenge control group, which is important in terms of reducing potential transmission to naive animals. Conclusions: The results show that a dose of 10 µg antigen in the vaccine will be optimal in terms of maximising efficacy, reducing shedding at parturition, and ensuring it is cost-effective to produce for commercial manufacture. Full article

Pathogen infection in animals and plants is recognized in a relatively similar manner by the interaction of pattern recognition receptors on the host cell surface with pathogen-associated molecular patterns on the pathogen surface. Previous work demonstrates that animal pathogenic bacteria can be recognized by plant receptors and alter transcriptome. In this work, we have hypothesized that exposure to human parasites, Cryptosporidium parvum and Giardia lamblia, would also trigger pathogen response in plants, leading to changes in transcriptome. Detached Arabidopsis leaves were exposed for one hour to heat-inactivated Cryptosporidia or Giardia. The transcriptome profile showed large changes in gene expression with significant overlap between two parasites, including upregulated GO terms “cellular response to chitin”, “response to wounding”, “response to oomycetes”, “defense response to fungus”, “incompatible interaction”, and “activation of innate immune response”, and downregulated GO terms “positive regulation of development”, “cell surface”, “regulation of organ growth”, “wax biosynthetic process”, “leaf and shoot morphogenesis”. Uniquely downregulated GO terms in response to Cryptosporidia were GO terms related to chromatin remodelling, something that was not reported before. To conclude, it appears that while Cryptosporidia or Giardia are not pathogens of Arabidopsis, this plant possesses various mechanisms of recognition of pathogenic components of parasites. Full article

Cultural organizations, such as museums, increasingly seek innovative ways to enhance their financial sustainability and attract diverse, global audiences. Implementing cryptocurrency payments and DeFi systems offers these institutions an opportunity to modernize their operations, streamline transactions, and boost digital marketing efforts, aligning with the growing demand for decentralized financial solutions. Using statistical analyses such as correlations and simple linear regression (SLR) models, combined with AnyLogic modeling, this study examines how integrating DeFi systems, including cryptocurrency payments, can improve the sustainable management of these institutions. The findings suggest that by adopting DeFi technologies, museums can enhance their digital marketing efficiency, increase engagement, and attract a broader audience. The analysis reveals that museums accepting cryptocurrency benefit from broader digital marketing factors, with referral and branded traffic significantly driving organic search, whereby paid social traffic correlates positively with paid strategies, and the authority score is largely influenced by organic traffic. In contrast, non-crypto museums rely more heavily on referral traffic and organic costs, with narrower marketing influences affecting their performance. Full article

As the fundamental unit of soil structure, soil aggregates play a crucial role in enhancing soil carbon and nitrogen storage, thereby supporting soil fertility and overall health, particularly in fragile karst regions. This study aims to quantify the effects of various ecological construction measures on soil aggregate stability, including focusing on geometric mean diameter (GMD), mean weight diameter (MWD), and K values, as well as aggregate−related organic carbon (SOC) and total nitrogen (TN), soil mechanical composition, and aggregate content. The ecological construction measures examined include plantation forests (Y7th–rgl), restored forests (Y6th–zr), fruit forests (Y6th–jgl), and contour reverse slope terraces (Y1th–crt). Compared to sloping farmland, contour reverse slope terraces, with their distinctive priority induction function, significantly increased the content of medium−fine particle aggregates, greater than 87%. Among the ecological construction measures, plantation forests exhibited the highest aggregate stability, with an average increase ranging from 8% to 157%. Notably, microaggregates, regardless of size, possessed the highest carbon and nitrogen contents, contributing significantly to soil carbon and nitrogen pools. Furthermore, both plantation and contour reverse slope terrace treatments demonstrated an equal contribution of carbon and nitrogen across all aggregate sizes. The partial least squares path modeling (PLS−PM) analysis indicates that land use type and the content of carbon and nitrogen pools are the primary factors influencing soil aggregate stability. These findings suggest that plantations are particularly effective in enhancing soil and water conservation in fragile karst areas, while the contour reverse slope terrace method shows potential for stabilizing soil structure over extended time scales due to its unique “preferential entrainment” function. Full article

The industrial emissions of volatile organic compounds (VOCs) are a major contributor to air pollution in urban areas. Previous studies on VOC emissions in industrial zones have primarily relied on in situ monitoring techniques, which pose significant challenges in capturing high emissions peaks and near-source measurements on regional scales. In this study, we employed mobile proton transfer reaction–time-of-flight–mass spectrometry (PTR–ToF–MS) to identify and characterize industrial VOC hotspots in a petrochemical park in eastern China, from June to September 2021. The average total VOC concentrations in the industrial zone were 131.5 ± 227.7 ppbv, approximately 48% higher than those in the background area (88.9 ± 63.3 ppbv), reflecting the substantial emissions from industrial hotspots. Oxygenated VOCs were the most abundant components in the industrial zone (83.2 ppbv). The overall OH reactivity, aerosol formation potential, and lifetime cancer risk of the industrial zone were also substantially higher than those in the background zone. These findings emphasize the need for targeted VOC emissions controls in industrial hotspots to mitigate air quality and health risks. Full article

Late-maturing hybrid citrus is a significant fruit that combines the best traits of both parents and is highly prized for its unique flavor. Not only can organic acids alter the flavor of citrus pulp, but they are also essential for cellular metabolism, energy conversion, and maintaining the acidbase balance in plant tissues. Although organic acids play a key role in the quality formation of citrus fruits, there is still insufficient research on the metabolic processes of organic acids in late-maturing hybrid citrus varieties. In this study, three late-maturing citrus varieties with different acidity levels, namely ‘Huangjinjia’ (HJ), ‘Kiyomi’ (QJ), and ‘Harumi’ (CJ), were selected to systematically investigate the metabolic regulation mechanism of organic acids in late-maturing citrus through transcriptome sequencing technology, combined with physiological and biochemical analyses. This study revealed gene expression differences related to organic acid synthesis and degradation. Through gene expression profiling, several genes closely associated with organic acid metabolism were identified, and a preliminary gene network related to the regulation of organic acid metabolism was constructed. The results showed that there were significant differences in the organic acid metabolic pathways between different varieties and growth stages of the fruit. Specifically, HJ had a higher TA content than QJ and CJ, primarily due to the significantly higher citric acid and malic acid contents in HJ compared to the other two varieties. Further analysis revealed that four gene modules showed a high correlation with the levels of major organic acids in the fruits. The genes involved in these modules are closely related to organic acid synthesis, degradation, and transport. Additionally, we also identified several key genes (AS1, BZP44, COL4, TCP4, IDD10, YAB2, and GAIPB) that might be involved in the regulation of organic acid metabolism. The functions of these genes could have a significant impact on the expression levels changes of enzymes related to organic acid metabolism. This study provides a foundation for exploring the intrinsic mechanisms regulating the organic acid content in late-maturing hybrid citrus fruits and contributes to the functional research of organic acids in late-maturing hybrid citrus and the molecular design of high-quality varieties. Full article

Carotenoids, the bright yellow, orange, and red pigments of many fruits and vegetables, are essential components of the human diet as bioactive compounds not synthesized in animals. As a staple crop potato has the potential to deliver substantial amounts of these nutraceuticals despite their lower concentration in tubers compared to edible organs of other plant species. Even small gains in tuber carotenoid levels could have a significant impact on the nutritional value of potatoes. This review will focus on the current status and future perspectives of carotenoid biofortification in potato with conventional breeding and biotechnological approaches. The high biodiversity of tuber carotenoid levels and composition is presented, with an emphasis on the under-exploited native germplasm that represents a wide reservoir of useful genetic variants to breed carotenoid-rich varieties. The following section describes the structural genes involved in carotenoid metabolism and storage known to have a major impact on carotenoid accumulation in potato, together with the strategies that harnessed their expression changes to increase tuber carotenoid content. Finally, the little information available on the regulation of carotenoid metabolism and the desirable future advances in potato carotenoid biofortification are discussed. Full article

The linkage between land tenure security and land quality improvement investment is crucial given that the land tenure security system is a widely applied policy tool for the protection of cultivation land in developing countries. Drawing on the triple land tenure security framework, this paper examines the impact of the de jure and the de facto land tenure security on farming households’ decisions about using organic fertilizer on their plots in China, based on a national survey dataset covering 2308 plots matched with 962 farming households across 8 provinces in China (Shangdong, Shangxi, Jilin, Zhejiang, Henan, Gansu, Hunan, and Sichuan) from January to July 2013. The empirical results show that the de facto land tenure security affected the use of organic fertilizer by the farming households positively. In comparison, the impact of the de jure land tenure security on the use of organic fertilizer by farming households was modest. It is suggested that the government should implement the policies effectively to promote de jure land tenure security and encourage farming households to make sustainable land investment. Full article

Cancer is the second leading cause of death globally and the estimated number of new cancer cases and deaths will be ∼30.2 million and 16.3 million, respectively, by 2040. These numbers cause massive, physical, emotional, and financial burdens to society and the healthcare system that lead to further research for a better and more effective therapeutic strategy to manage cancer. Metal–organic frameworks (MOFs) are promising alternative approaches for efficient drug delivery and cancer theranostics owing to their unique properties and the direct transportation of drugs into cells followed by controlled release, but they suffer from certain limitations like rigidity, poor dispersibility, fragility, aggregation probability, and limited surface accessibility. Therefore, MOFs were conjugated with polymeric hydrogels, leading to the formation of MOF-based hydrogels with abundant absorption sites, flexibility, and excellent mechanical properties. This review briefly describes the different strategies used for the synthesis and characterization of MOF-based hydrogels. Further, we place special emphasis on the recent advances in MOF-based hydrogels used to manage different cancers. Finally, we conclude the challenges and future perspectives of MOF-based hydrogels. We believe that this review will help researchers to develop more MOF-based hydrogels with augmented anticancer effects, enabling the effective management of cancer even without adverse effects. Full article

This study presents a comprehensive phyto- and histochemical analysis of three Colchicum species: Colchicum autumnale L., the Balkan endemic Colchicum bivonae Guss., and the Bulgarian endemic Colchicum diampolis Delip. et Česchm. Using gas chromatography-mass spectrometry (GC-MS), 66 metabolites were identified, encompassing free amino, organic, phenolic, and fatty acids, sugars, and alkaloids, which were distributed among various plant parts. Organ-specific metabolic patterns revealed that corms and seeds are particularly rich in alkaloids, supporting their roles in chemical defense and survival during dormancy. Conversely, flowers, leaves, and capsules were enriched with energy-related and phenolic compounds, playing critical roles in reproduction and stress tolerance. Histochemical investigations localized alkaloids predominantly in the endosperm of seeds, parenchyma of corms, and vascular bundles of flowers. Notably, the endemic C. bivonae and C. diampolis displayed unique chemical profiles. Moderate acetylcholinesterase inhibitory activity (AChE) was observed across various plant organs. Statistical analyses demonstrated significant interspecies and organ-specific chemical differentiation, with certain metabolites serving as key markers. These findings enhance our understanding of the chemical composition, organ specialization, and potential as a source of new biomolecules in these Colchicum species. They underscore the ecological and pharmacological importance of endemic taxa and provide a framework for future research into their sustainable utilization and potential bioactivities. Full article

This study explores the impact of geographical origin, harvest time, and cooking on the volatile organic compound (VOC) profiles of wild and reared seabream from the Adriatic and Tyrrhenian Seas. A Proton Transfer Reaction–Time of Flight–Mass Spectrometry (PTR-ToF-MS) allowed for VOC profiling with high sensitivity and high throughput. A total of 227 mass peaks were identified. Principal component analysis (PCA) showed a clear separation between cooked and raw samples, with cooking causing a significant increase in 64% of VOCs, especially hydrogen sulphide, methanethiol, and butanal. A two-way ANOVA revealed significant effects of origin, time, and their interaction on VOC concentration, with 102 mass peaks varying significantly based on all three factors. Seasonal effects were also notable, particularly in reared fish from the Adriatic Sea, where compounds like monoterpenes and aromatics were higher during non-breeding months, likely due to environmental factors unique to that area. Differences between wild and reared fish were influenced by lipid content and seasonal changes, impacting the VOC profile of seabream. These findings provide valuable insights into how cooking, geographical origin, and seasonality interact to define the flavour profile of seabream, with potential applications in improving quality control and product differentiation in seafood production. Full article

Nanoparticles (NPs) are excellent antibacterial agents due to their ability to interact with microorganisms at the cellular level. However, their antimicrobial capacity can be limited by their tendency to agglomerate. Functionalizing NPs with suitable ligands improves their stability and dispersion in different media and enhances their antibacterial activity. The present work studied the functionalization of SiO₂ NPs using the sonochemical method and the Influence of organic ligands on antimicrobial activity (AA). The organic ligands studied were citric acid (CA) and monoethanolamine (MEA). X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) results confirmed the amorphous structure of SiO₂ NPs and their functionalization. Thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS) showed that functionalization with MEA (SiO₂-MEA NPs) is more favored compared to AC (SiO₂-CA NPs), and the organic ligand content was 34.42% and 28.0%, respectively. Fourier-transform infrared spectroscopy (FTIR) and RAMAN spectroscopy results confirmed the functionalization of NPs through the presence of carboxyl and amino groups. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and zeta potential results showed that functionalization of SiO₂ NPs helped to improve their dispersion and prevent their agglomeration. Furthermore, the results of antibacterial activity against Staphylococcus aureus and Escherichia coli showed that the functionalization provided a significant improvement in the antibacterial activity (AA) of the SiO₂ NPs, where the SiO₂-CA NPs showed the highest activity, with a 99.99% inhibition percentage at concentrations of 200 ppm against both E. coli and S. aureus strains. The AA is maintained at high concentrations of 1200 ppm, which is essential in applications requiring high percentages of biocidal NPs, such as marine coatings. Full article

This study tracks changes in commercial buildings in Yeonnam-dong that have undergone commercial transformation, examining these shifts within the context of urban organization and their relationships with economic and socio-cultural flows. It also analyzes the architectural elements that reflect the placeness characteristics of an area. On-site surveys were conducted to assess the continuity and discontinuity in the characteristics of 62 renovated and 40 newly constructed buildings. Additionally, shifts in social perceptions regarding physical changes were explored through an analysis of commercial districts and interviews with visitors, store owners, and architects. Yeonnam-dong has a distinct physical environment, as well as economic, social, and cultural characteristics that distinguish it from mainstream areas, such as large apartment complexes and typical commercial zones. The area, with its winding alleyways from the pre-modern period and low-rise housing—marginalized from large-scale development—evoked nostalgia and created a distinctive sense of authenticity or placeness, which was further enhanced by the presence of exotic restaurants, cafés, ateliers, and creative activities of early gentrifiers. However, rapid commercialization has led to the displacement of these early gentrifiers, an influx of new investments and construction, and shifts in commercial flows. Consequently, the area’s authenticity has become diluted, and visitors’ perceptions of it have changed. Despite this, architectural elements observed in renovated buildings are preserved and incorporated into newly constructed ones. Features such as semi-basements, exterior straight stairs, and brick façades continue to reflect the locality. Detached and multi-household homes from the 1960s to the 1990s have evolved into a new hybrid commercial–residential form designed for commercial purposes. This is a response to small plot sizes in Korean residential areas and reflects the current economic and cultural demands of commercial gentrification. The urban and architectural characteristics of this area are not only a unique physical environment but also hold socio-cultural significance as ordinary heritage. Therefore, this study proposes institutional measures to preserve urban and architectural continuity while controlling the ongoing commercialization. Full article