Search Results (341)

Background/Objectives: It remains inconclusive whether or not smoking is associated with an increased risk of fatty liver disease (FLD). We investigated the association between smoking and the risk of FLD by using a meta-analysis of cohort studies. Methods: PubMed and EMBASE were searched using keywords from their inception to September 2023 to identify relevant studies. Results: Out of 806 articles searched from the databases, a total of 20 cohort studies were included in the final analysis. In the meta-analysis, smoking was significantly associated with an increased risk of FLD (odds ratio/relative risk/hazard ratio, 1.14; 95% confidence interval, 1.05–1.24; n = 20). Subgroup analyses showed a significant positive association between them in prospective cohort studies (odds ratio/relative risk/hazard ratio, 1.15; 95% confidence interval, 1.05–1.18; n = 5) but not in retrospective cohort studies and cross-sectional studies based on cohort studies. In the subgroup meta-analysis by gender in Asians, smoking significantly increased the risk of FLD in men, while there was no significant association between FLD and smoking in women. Conclusions: This meta-analysis showed that smoking increases the risk of FLD. In addition to well-known risk factors of FLD, clinicians should recommend smoking cessation for the management of FLD. Full article

Bisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, simple, and ”green” DLLME method was used, achieving mean recovery values in the range of 62%–98% with relative standard deviations between 2% and 6% for all analytes. Quantitative analysis of bisphenols in the samples was then performed by LC–MS/MS on a triple quadrupole (QqQ) mass spectrometer and electrospray ionization (ESI-/ESI+) was applied in the negative and positive ion modes, respectively. The LODs and LOQs ranged from 0.04 ng/mL to 0.37 ng/mL and 0.12 ng/mL to 1.11 ng/mL, respectively. Pericardial fluid was collected from patients with coronary artery disease during coronary artery bypass surgery. Bisphenol residues were identified and quantified in samples from 19 patients. The procedure was successfully applied to the biomonitoring of free forms of 14 bisphenols in pericardial fluid. After statistical examination of the relationships between the selected variables, a strongly positive correlation was found between creatinine kinase and troponin I, as well as the number of venous anastomoses, circulation time, and clamp cap time. Full article

Fumonisins, a class of mycotoxins predominantly produced by Fusarium species, represent a major threat to food safety and public health due to their widespread occurrence in staple crops including peanuts, wine, rice, sorghum, and mainly in maize and maize-based food and feed products. Although fumonisins occur in different groups, the fumonisin B series, particularly fumonisin B1 (FB1) and fumonisin B2 (FB2), are the most prevalent and toxic in this group of mycotoxins and are of public health significance due to the many debilitating human and animal diseases and mycotoxicosis they cause and their classification as by the International Agency for Research on Cancer (IARC) as a class 2B carcinogen (probable human carcinogen). This has made them one of the most regulated mycotoxins, with stringent regulatory limits on their levels in food and feeds destined for human and animal consumption, especially maize and maize-based products. Numerous countries have regulations on levels of fumonisins in foods and feeds that are intended to protect human and animal health. However, there are still gaps in knowledge, especially with regards to the molecular mechanisms underlying fumonisin-induced toxicity and their full impact on human health. Detection of fumonisins has been advanced through various methods, with immunological approaches such as Enzyme-Linked Immuno-Sorbent Assay (ELISA) and lateral flow immunoassays being widely used for their simplicity and adaptability. However, these methods face challenges such as cross-reactivity and matrix interference, necessitating the need for continued development of more sensitive and specific detection techniques. Chromatographic methods, including HPLC-FLD, are also employed in fumonisin analysis but require meticulous sample preparation and derivitization due to the low UV absorbance of fumonisins. This review provides a comprehensive overview of the fumonisin family, focusing on their biosynthesis, occurrence, toxicological effects, and levels of contamination found in foods and the factors affecting their presence. It also critically evaluates the current methods for fumonisin detection and quantification, including chromatographic techniques and immunological approaches such as ELISA and lateral flow immunoassays, highlighting the challenges associated with fumonisin detection in complex food matrices and emphasizing the need for more sensitive, rapid, and cost-effective detection methods. Full article

The prevalence of coniferous trees in the forest landscapes of northeastern Siberia is conditioned by their high frost resistance. The Kajander larch (Larix cajanderi Mayr), which can survive under natural conditions (down to −60 °C) in the cryolithozone of Yakutia, is the dominant forest-forming species. We hypothesise that our study using HPTLC–UV/Vis/FLD, TLC–GC/FID, and GC–MS methods of seasonal features of the lipid profile of Kajander larch tissues will bring us closer to understanding the mechanisms of participation of lipid components in the adaptation of this valuable tree species to the cold climate of the cryolithozone. Rare delta5-unsaturated polymethylene-interrupted fatty acids (∆5-UPIFA) were identified in the fatty acids (FAs) of L. cajanderi shoots, including 18:2(Δ5.9) (taxoleic), 18:3(Δ5.9.12) (pinolenic), and 18:4(Δ5.9.12.15) (coniferonic). It was found that the content of ∆5-UPIFA in L. cajanderi shoots markedly increased (1.5-fold, representing up to 23.9% of sum FAs) during the autumnal transition of trees to dormancy. It was observed that the ranges of low temperatures experienced during the prolonged winter period primarily determined the structural diversity of membrane lipids and their constituent FAs during the cold adaptation of L. cajanderi. The results obtained can be used for the selection of molecular markers of cold tolerance in woody plants, including fruit trees. Full article

The urothelium and lamina propria (LP) contribute to sensations of bladder fullness by releasing multiple mediators, including prostaglandins (PGs) and adenosine 5′-triphosphate (ATP), that activate or modulate functions of cells throughout the bladder wall. Mediators that are simultaneously released in response to bladder distention likely influence each other’s mechanisms of release and action. This study investigated whether PGs could alter the extracellular hydrolysis of ATP by soluble nucleotidases (s-NTDs) released in the LP of nondistended or distended bladders. Using an ex vivo murine detrusor-free bladder model to access the LP during bladder filling and a sensitive HPLC-FLD detection methodology, we evaluated the decrease in ATP and the increase in adenosine 5′-diphosphate (ADP), adenosine 5′-monophosphate (AMP), and adenosine by s-NTDs released in the LP. Endogenous PGE₂ increased the spontaneous but not the distention-induced release of s-NTD via EP2 and EP3 prostanoid receptors, whereas exogenous PGE₂ increased the spontaneous s-NTD release via EP3, EP4, and FP receptors and the distention-induced s-NTD release via EP1-4 and FP receptors. Endogenous PGF_2α, PGD₂, and PGI₂ did not change the s-NTD release. Exogenous PGD₂ increased the spontaneous s-NTD release via DP2 receptors and the distention-induced s-NTD release via DP1 and DP2 receptors. Exogenous PGF_2α increased the spontaneous but not the distention-induced release of s-NTD via FP receptors. It is possible that higher concentrations of PGE₂, PGF_2α, and PGD₂ (as expected in inflammation, bladder pain syndrome, or overactive bladder) potentiate the release of s-NTDs and the consecutive degradation of ATP as a safeguard mechanism to prevent the development of excessive bladder excitability and overactivity by high amounts of extracellular ATP. Full article

This study assessed the technological potential and bioactive compounds present in purple yampee (Dioscorea trifida L.f.) lyophilized powder, peeled and whole flour, as well as the tuber peel, starch residual fiber, and wastewater mucilage. Although most values approached neutrality, flour showed a lower pH and high density, while greater acidity was observed in the mucilage. Color differed statistically and perceptibly between all samples, with similar values of °hue to purple flours from other sources, and the maximum chroma was found in lyophilized pulp and lightness in fiber. Average moisture levels around 7.2% and water activity levels of 0.303 (0.194 for whole flour) in fractions suggested favorable storability, while the interaction of the powders with water was similar to other root and tuber powdered derivatives. Yampee periderm had the highest swelling power, oil absorption capacity, water holding capacity, and absorption index and capacity. Mucilage had a higher solubility index and outstanding emulsion activity, greater than 90%. Twelve anthocyanins, with new reports of petunidin derivatives for the species, and more than 30 phytochemicals were identified through advanced liquid chromatography techniques. The greatest amounts of pinitol and myo-inositol were found in the mucilage, and sucrose, glucose, and fructose prevailed in the other powders. Successfully characterized yampee fractions showed high potential as functional food ingredients. Full article

High-strength steels such as Dual Phase (DP), Transformation-Induced Plasticity (TRIP), and Twinning-Induced Plasticity (TWIP) steels have gained importance in automotive applications due to the potential for weight reduction and increased performance in crash tests. However, as resistance increases, there is also an increase in springback due to residual stresses after the forming process. This is mainly because of the greater elastic region of these materials and other factors associated with strain hardening, such as the Bauschinger effect, that brings theory of kinematic hardening to mathematical modeling. This means that finite element software must consider these properties so that the simulation can accurately predict the behavior. Currently, this knowledge is still not widespread since it has never been used in conventional materials. Additionally, engineers and researchers use the Forming Limit Diagram (FLD) curve in their studies. However, it does not fully represent the actual failure limit of materials, especially in high-strength materials. Based on this, the Fracture Forming Limit Diagram (FFLD) curve has emerged, which proposes to resolve these limitations. Thus, this review aims to focus on how finite element methods consider all these factors in their modeling, especially when it comes to the responses of high-strength steels. Full article

In this study, we developed a colloidal gold immunochromatographic strip (CGIS) method that used the matrix-matched calibration curves of contamination ratio models to quantitatively determine the total aflatoxin in five herbal medicines. This approach addresses issues related to false results and poor accuracy associated with conventional methods. The CGIS was analyzed using a Vertu touch reader, and the matrix-matched calibration was established based on the absorbance ratios of the T and C lines, as well as the logarithmic values of the total aflatoxin concentrations. The total aflatoxins could be accurately and digitally detected from 2.5 to 40 μg/kg, and the LOD of total aflatoxins was 1 μg/kg in the five herbal medicines. The recovery rates from the spiked samples ranged from 65.1% to 98.6%, and the RSD was less than 16.9%. A total of 229 samples were analyzed by both CGIS and HPLC-FLD, with agreement ranging from 78.4% to 132.6% (Arecae semen), 82.6% to 133.0% (Nelumbinis semen), 79.9% to 117.9% (Coicis semen), 78.1% to 119.0% (Platycladi semen), and 76.1% to 123.0% (Ziziphi spinosae semen). This process for the discrimination of the CGIS results was established to assess if samples met the requirement of aflatoxin limits, which could save approximately 75% in time and reduce the workload of retesting by a designated confirmatory reference method to less than 10%. This study demonstrated that the application of matrix-matched calibration curves based on contamination ratio models to CGIS can effectively enhance the rapid quantitative determination capability of total aflatoxins in herbal medicine matrices. Full article

The paper presents results of experimental investigations of the influence of temperature on the effectiveness of passive vibration isolation. Two types of viscoelastic materials (butyl rubber and bituminous material) were tested. In the performed vibration analysis, the Oberst beam made out of aluminum alloy with a damping material in a Free Layer Damping (FLD) configuration was used. The experimental modal analysis was performed using the Unholtz-Dickie UDCO TA-250 vibration system. To investigate the influence of temperature on the effectiveness of passive vibration isolation, an isothermal cooling chamber (using Peltier cells) was designed and constructed. The tests were carried out in a wide frequency range from 40 Hz to 4000 Hz, at a constant sweep rate, in a temperature range from −2 °C to 22 °C. Miniature piezoelectric acceleration sensors were used to determine the acceleration of the beam and the exciter head. The analysis of accelerations of both the object and the shaker head allowed for the determination of a Frequency Response Function (FRF) for the beam. The course of FRF was used to determine the resonance frequencies and the vibration amplitudes of the beam damped with bituminous material and butyl rubber at various temperatures. The loss factor η, calculated for each resonance using the generalized half-power method (n-dB method), was used as an indicator of damping intensity. The research results presented in this work (important from scientific point of view) also have utilitarian significance and can be used in the design of more quiet and comfortable motor vehicles, railway wagons and aircraft structures. Full article

This study examines the specimen size-dependent deformation behavior of commercially pure titanium grade 4 (cp-Ti grade 4) sheets under tension, with strain paths between uniaxial tension (UT) and plane-strain tension and compares the results with cyclic bending under tension (CBT) data. Specimens of varying widths (11.7, 20, 60, 100, and 140 mm) were tested in both rolling (RD) and transverse (TD) directions. The research employed digital image correlation for full-field strain measurements, finite element simulations, and fracture surface thickness data. Contrary to traditional forming concepts, i.e., the forming limit diagram (FLD) has the lowest major strain at the plane-strain condition, and the fracture forming limit has decreased major strain with increasing (less negative) minor strain, wider specimens exhibited higher major strains at strain localization and fracture under UT. In contrast, CBT findings showed decreased formability with increasing width, i.e., closer to plane-strain deformation, as expected. Strain distribution analyses revealed a transition from nearly uniform deformation in narrow specimens to multiaxial strain states in wider specimens. Thickness measurements along the fracture surface revealed a steeper profile in UT compared to CBT, indicating more localized deformation and necking in UT. In comparison with AA6016-T4, the cp-Ti grade 4 showed greater thickness, suggesting lower susceptibility to localized thinning. Strong anisotropy was observed between the RD and TD, with TD specimens showing higher formability and steeper thickness gradients in UT. Strain fields, along with thickness reduction and adiabatic heating, are used to rationalize the observed width-sensitive deformation behavior of cp-Ti sheets. Notably, CBT improved overall formability compared to UT due to its ability to distribute strain more evenly and delay critical necking. The contrasting trends between simple UT and CBT emphasize the relationship between loading conditions, specimen geometry, and material behavior in determining formability. These findings highlight the ability of the CBT test to create known and desired deformation effects, i.e., lower major strain at failure with increasing specimen width, and more uniform deformation, i.e., consistent thinning across the specimen width, for cp-Ti. Given the observed effects of width in UT, the selection of the testing method is critical for cp-Ti to ensure that results reflect expected material behavior. Full article

In recent years, the detection of nitrosamine precursors has become an important issue for regulatory authorities such as the European Medicines Agency (EMA) and the Food and Drug Administration (FDA). The present study provides a pre-column derivatization method for the analysis of dimethylamine (DMA) and diethylamine (DEA) in pharmaceutical products using HPLC and a fluorescence detector. Appropriate chromatographic parameters, including mobile phase composition (organic solvent, buffer, pH), elution type, flow rate, temperature, and λexcitation/emission, were investigated. Analysis was performed at λ_excitation = 450 nm and λ_emission = 540 nm on a C₁₈ column (at 40 °C) using gradient elution as a mobile phase with Eluent A: Phosphoric Acid Buffer (20 mM, pH = 2.8) and Eluent B: methanol, with a flow of 0.8 mL/min. The method was validated according to ICH specifications in terms of linearity (0.5–10 ng/mL for DMA and 5–100 ng/mL for DEA), specificity, and robustness, as well as repeatability, intermediate precision (%RSD < 2.9), and accuracy (% recovery 98.2–102.0%). The derivatization process was optimized using the “Crossed D-Optimal” experimental design procedure, where one mixture component was cross-correlated with two factors. The stability of the samples was studied over a period of one month. To process the samples (pharmaceuticals), various purification techniques were tried using solid/liquid or liquid/liquid extraction with dichloromethane. Finally, a straightforward solid-phase extraction (SPE, C₁₈) method was chosen prior to derivatization. The method was successfully applied, since the extraction recoveries were >81.6% for DMA (0.5 ppm) and >81.1% for DEA (5 ppm). Based on the results obtained and the available literature, the scientific community seeks, by proposing flexible analytical methods, to delimit the problem of nitrosamines. Full article

Aflatoxin M₁ (AFM₁) in milk poses a significant threat to human health. This study examined the capacity of Bacillus licheniformis CotA laccase to oxidize AFM₁. The optimal conditions for the CotA laccase-catalyzed AFM₁ oxidation were observed at pH 8.0 and 70 °C, achieving an AFM₁ oxidation rate of 86% in 30 min. The Km and Vmax values for CotA laccase with respect to AFM₁ were 18.91 μg mL⁻¹ and 9.968 μg min⁻¹ mg⁻¹, respectively. Computational analysis suggested that AFM₁ interacted with CotA laccase via hydrogen bonding and van der Waals interactions. Moreover, the oxidation products of AFM₁ mediated by CotA laccase were identified as the C3-hydroxy derivatives of AFM₁ by HPLC-FLD and UPLC-TOF/MS. Toxicological assessment revealed that the hepatotoxicity of AFM₁ was substantially reduced following oxidation by CotA laccase. The efficacy of CotA laccase in removing AFM₁ in milk was further tested, and the result showed that the enzyme agent achieved an AFM₁ removal rate of 83.5% in skim milk and 65.1% in whole milk. These findings suggested that CotA laccase was a novel AFM₁ oxidase capable of eliminating AFM₁ in milk. More effort is still needed to improve the AFM₁ oxidase activity of CotA laccase in order to shorten the processing time when applying the enzyme in the milk industry. Full article

This study aimed to evaluate the marginal and internal adaptation of CAD/CAM crowns milled using two different milling protocols (fine or extra-fine) within a 4-axis milling machine. The crowns were fabricated from lithium disilicate ceramic (IPS e.max CAD) and resin composite (Tetric CAD), assessing their fit in various regions. The crowns (N = 40, n = 10) were milled from lithium disilicate and resin composite using a CEREC Primemill unit. Four groups were formed based on the material and milling protocol: EFLD (extra-fine lithium disilicate), FLD (fine lithium disilicate), EFRC (extra-fine resin composite), and FRC (fine resin composite). The crowns were measured using the replica technique, evaluating internal and marginal adaptation in 18 measuring points per specimen. Data were statistically analyzed using ANOVA and Tukey’s test. Resin composite crowns demonstrated a significantly better internal fit compared to lithium disilicate (p < 0.001). Marginal and internal measurements for resin composites were consistently smaller across regions compared to lithium disilicate. No significant differences were found between milling protocols except for the axial wall region (p = 0.001), where extra-fine milling resulted in smaller values. Resin composite crowns exhibited superior internal fit compared to lithium disilicate, regardless of milling protocol. Both the fine and extra-fine milling protocols had minimal impact on adaptation, except at the axial wall region, with both protocols promoting adequate results overall. Full article

Ochratoxin A (OTA) is a nephrotoxic mycotoxin that commonly contaminates maize, posing significant health risks to both poultry and humans. In this study, a rapid and sensitive method utilizing ultra-performance liquid chromatography coupled with fluorescence detection (UPLC-FLD) was developed for the quantification of OTA levels in maize. The method utilizes immunoaffinity column purification for improved specificity. Accuracy and precision were validated in line with European Union Reference Laboratory (EURL-MP) guidelines, meeting regulatory standards for linearity, trueness, detection and quantification limits, precision, and uncertainty, as per European Commission Regulation (EC) No. 401/2006 and its amendments. The method demonstrated an average recovery rate of 116.78% for maize, with RSD_wR values (within-laboratory reproducibility) of 12.72%. Furthermore, OTA occurrence and its possible effects were investigated in several farms in South Romania, where necropsy and histopathological analyses of poultry revealed severe kidney damage, including renal tubular degeneration. Full article

Background/Objectives: Carrot is a major root vegetable in the Apiaceae owing to its abundant carotenoids, antioxidants, vitamins, and minerals. The modern dark orange western carrot was derived from sequential domestication events from the white-rooted wild form to the pale orange-, purple-, or yellow-rooted eastern carrot. Genetic and molecular studies between eastern and western carrots are meager despite their evolutionary relatedness. Methods: Twelve RNA seq libraries obtained from distinct eastern and western cultivars at vegetative and reproductive developmental stages were utilized to identify differentially expressed genes (DEGs) to decode the key molecular genetic changes in carotenoid and flowering pathways. Results: In the carotenoid pathway, an upregulation of the PSY, CRTISO, and LCYE genes was observed in the western cultivar, while the eastern cultivar exhibited a higher abundance of downstream enzymes, particularly CCD and NCED1. These later enzymes are crucial in linking apocarotenoids and xanthin-mediated ABA signaling. In the flowering pathway, we noted a greater expression of DEGs associated with the photoperiod and vernalization pathways in the western cultivar. In contrast, the eastern cultivar displayed a dominance of genes from the autonomous pathway (FLD, LD, FLK, and PEBP) that function to repress FLC. The experimental validation of 12 key genes through quantitative real-time PCR further confirms their functional role in carrots. Conclusions: The identified key regulatory genes in these major pathways are valuable for designing breeding strategies for manipulating carotenoid content and flowering time while developing climate-specific carrots. The knowledge of carotenoid and flowering pathways is advantageous in producing nutritionally improved roots and seeds in carrots across diverse climates. Full article