Sensing Plant Physiology and Environmental Stress by Automatically Tracking F_j and F_i Features in PSII Chlorophyll Fluorescence Induction

Following a step excitation, chlorophyll fluorescence (ChlF) from photosystem II (PSII) of a dark-adapted photosynthetic organism exhibits the well-known OJIP pattern. The OJIP induction has been widely used in plant science and agriculture engineering. While the J and I phases are related to transitions of photochemical reaction redox states, characteristic fluorescence intensities at the two phases (F_j and F_i ) are often treated at fixed time points in routine measurement and thus do not account for variations in plant and experimental conditions, this (1) neglects the differences in the time of appearance of these phases, which is potentially useful information for characterizing plant status and environmental factors, and (2) leads to errors in measured F_j and F_i values in the many publications. In this work, an alternative method for consistent measurement of F_j and F_i was presented. The proposed method measures the curvatures in the OJIP curve and automatically tracks the characteristic transition points under variable sample and experimental conditions. Experiments were carried out to demonstrate the concept and classification capabilities of the method. This research has established a new framework to analyze ChlF and has enhanced the application capability of ChlF. It is expect useful in analysis ChlF from PSII.