The multilayer perceptrons (MLPs) are widely used in many fields, however, singularities in the parameter space may seriously influence the learning dynamics of MLPs and cause strange learning behaviors. Given that the singularities are the subspaces of the parameter space where the Fisher information matrix (FIM) degenerates, the FIM plays a key role in the study of the singular learning dynamics of the MLPs. In this paper, we obtain the analytical form of the FIM for unipolar activation function-based MLPs where the input subjects to the Gaussian distribution with general covariance matrix and the unipolar error function is chosen as the activation function. Then three simulation experiments are taken to verify the validity of the obtained results.