Quantitative analysis of copy number variants based on real-time LightCycler PCR

Curr Protoc Hum Genet. 2014 Jan 21:80:7.21.1-7.21.8. doi: 10.1002/0471142905.hg0721s80.

Authors

Lijiang Ma¹, Wendy K Chung^{1

2}

Affiliations

¹ Department of Pediatrics, Columbia University, New York, New York.
² Department of Medicine, Columbia University, New York, New York.

Abstract

Quantitative real-time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes, which are employed to measure gene expression or gene quantification including contiguous gene deletions or duplications. A simple method is described here to quantify DNA copy number from human samples.

Keywords: CNV; copy number analysis; qPCR.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

DNA Copy Number Variations / genetics*
DNA Primers
Gene Deletion
Gene Dosage
Genetic Predisposition to Disease
Genetic Testing / methods*
Humans
Real-Time Polymerase Chain Reaction / methods*

Substances

DNA Primers

Grants and funding

U01 HL098163/HL/NHLBI NIH HHS/United States