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Induction of hepatic tyrosine aminotransferase in vivo by derivatives of cyclic adenosine 3':5'-monophosphate

J Biol Chem. 1975 Jan 25;250(2):426-31.

Abstract

A number of 8- and N6-SUBSTITUTED DERIVATIVES OF CYCLIC ADENOSINE 3':5'-MONOPHOSPHATE-DEPENDENT PROTEIN KINASE, AND AS SUBSTRATES FOR RAT LIVER CYCLIC NUCLEOTIDE PHOSPHODIESTERASE. All of the analogs tested were able to induce the transaminase. The induction by the analogs was shown to be the result of an actual increase in the amount of enzyme, and the mechanism of induction was an increase in the rate of synthesis of the transaminase. The induced enzyme appeared to be immunologically similar to the non-induced enzyme. A good correlation was found to exist between the dose that produced 50% of maximal induction and a combination of the activation constant for cyclic adenosine 3':5'-monophosphate-dependent protein kinase by the analog and its susceptibility to hydrolysis by cyclic nucleotide phosphodiesterase. These data suggest that the phosphorylation of some site is involved in the mechanism by which cyclic adenosine 3':5'-monophosphate affects the rate of synthesis of tyrosine aminotransferase.

MeSH terms

  • Animals
  • Bucladesine / pharmacology
  • Butyrates / pharmacology
  • Cyclic AMP / analogs & derivatives*
  • Cyclic AMP / pharmacology
  • Enzyme Activation / drug effects
  • Enzyme Induction / drug effects
  • Liver / enzymology*
  • Male
  • Phosphoric Diester Hydrolases / metabolism
  • Protein Kinases / metabolism
  • Rats
  • Structure-Activity Relationship
  • Tyrosine Transaminase / biosynthesis*

Substances

  • Butyrates
  • Bucladesine
  • Cyclic AMP
  • Tyrosine Transaminase
  • Protein Kinases
  • Phosphoric Diester Hydrolases