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Observation of plastoquinone kinetics in photosystem II from delayed fluorescence measurements

IET Syst Biol. 2010 Jan;4(1):90-8. doi: 10.1049/iet-syb.2008.0157.

Abstract

Attempts to account for the variations in photosystem II (PSII) under general conditions result in non-linear and cumbersome models that are difficult to validate and render few insights about the system kinetics. In this research, the authors experimentally show that under certain conditions, linear-system techniques could be applied to advantage for probing some basic kinetic characteristics of the plastoquinones (PQs). The PQ redox states of the reaction centres were represented in a conditionally linear model structure with delayed fluorescence (DF) as a measurable output. DF data were acquired for different plant samples and conditions. After least-squares parameter optimisation, not only could the model closely describe the measured DF, but more significantly, the estimated parameters correctly reflected the expected changes induced by drought or [3-(3,4-dichlorophenyl)-1,1-dimethylurea] (DCMU) stress. Analysis showed that for short-pulse illumination, the PQ kinetic states of the reaction centres in an initially dark-adapted plant leaf can be represented as a time-invariant bilinear system in a five-dimensional state space. The system becomes linear for constant illuminations, but the system matrix and the kinetic behaviour are illumination dependent. In particular, the system behaves differently between lights-on and lights-off conditions. The simplicity of the model structure, nonetheless, permits observation and analysis of the PQ kinetics of PSII reaction centres from DF measurements by using linear-system techniques.

MeSH terms

  • Computer Simulation
  • Light
  • Metabolic Clearance Rate
  • Models, Biological*
  • Photosystem II Protein Complex / physiology*
  • Photosystem II Protein Complex / radiation effects
  • Plant Leaves / physiology*
  • Plant Leaves / radiation effects
  • Plastoquinone / metabolism*
  • Radiation Dosage
  • Spectrometry, Fluorescence / methods

Substances

  • Photosystem II Protein Complex
  • Plastoquinone