WO2024222338A1

WO2024222338A1 - Method for detecting flow path, detection apparatus, and computer-readable storage medium

Info

Publication number: WO2024222338A1
Application number: PCT/CN2024/083451
Authority: WO
Inventors: 吴平; 万雪峰; 宋祺
Original assignee: 深圳市真迈生物科技有限公司
Priority date: 2023-04-28
Filing date: 2024-03-25
Publication date: 2024-10-31
Also published as: CN117433716B; CN117433716A

Abstract

A method for detecting a flow path, wherein the flow path comprises a first pipeline (11), a second pipeline (13) and a valve body (12), the valve body (12) is selectively communicated with the first pipeline (11) and the second pipeline (13), a gas sensing device (14) is arranged on the second pipeline (13), and the gas sensing device (14) sends a signal indicating the presence of gas at a predetermined position of the second pipeline (13) that is filled with fluid. The method comprises: introducing a gas into the flow path filled with fluid, comprising introducing the gas into the first pipeline, and introducing the fluid into the first pipeline so that the gas enters the flow path (a); receiving a signal from the gas sensing device (b); determining the amount of the fluid entering the flow path from the time when the fluid enters the first pipeline to the time when the signal from the gas sensing device is received (c); and comparing the amount of the fluid entering the flow path with a standard value to obtain a comparison result, and determining, on the basis of the comparison result, whether the flow path is anomalous, wherein the standard value reflects the amount of fluid entering the normal flow path under the same conditions (d). By means of the method, whether a flow path is anomalous can be quickly and accurately determined. Also provided are a detection apparatus for a flow path, and a computer-readable storage medium.

Description

Method for detecting flow path, detection device and computer readable storage medium

This application claims the priority of Chinese patent application CN202310484069.5 filed on April 28, 2023. The entire contents of the above Chinese patent application are incorporated into this application by reference.

Technical Field

The present application relates to the technical field of fluids, and in particular to a method for detecting a flow path, a detection device, and a computer-readable storage medium.

Background Art

In the related art, automated equipment generally includes a fluid circuit, which generally includes connected pipes, valves, pumps, etc. to control the flow of fluid from one location to another, so as to achieve automated fluid manipulation.

For example, in nucleic acid detection platforms such as gene sequencers, the liquid circuit is part of the automated sequencing system. During the sequencing process, the liquid circuit transports the reagents required for sequencing from the reagent storage device to a specified location such as a reaction device (such as a flow cell) carrying the molecule to be tested for biochemical reaction. Generally, sequencing operations require the control of the liquid circuit to continuously input or output related solutions or reagents to achieve biochemical reactions and signal detection. Therefore, the continuous operation of the liquid circuit system includes continuous pressure, which is inevitably worn out or aged or even damaged. This may cause liquid leakage or contamination during the operation, thereby affecting the acquisition of sequencing results or the effective operation or service life of other mechanical components or even the entire detection platform.

Therefore, before the detection platform is operated, it is usually necessary to test the air tightness of the liquid circuit therein. How to realize liquid circuit detection, convenient, fast and accurate detection is a problem worthy of attention.

Application Contents

In order to solve at least one of the technical problems existing in the prior art, the present application provides a method for detecting a flow path, a detection device, and a computer-readable storage medium, which are described in detail below.

The technical solution of the method for detecting flow path provided in the embodiment of the present application is as follows:

A method for detecting a flow path, wherein the flow path includes a first pipeline, a second pipeline and a valve body, the valve body selectively connects the first pipeline and the second pipeline, a gas sensing device is provided on the second pipeline, and the gas sensing device sends a signal indicating that gas exists at a predetermined position of the second pipeline filled with fluid, the method comprising:

a) allowing gas to enter a flow path filled with fluid, including allowing gas to enter a first conduit, and allowing fluid to enter the first conduit to allow gas to enter the flow path;

b) receiving a signal from a gas sensing device;

c) determining the amount of fluid entering the flow path from the time the fluid enters the first conduit to the time the signal from the gas sensing device is received; and

d) comparing the amount of fluid entering the flow path with a standard value to obtain a comparison result, and determining whether the flow path is abnormal based on the comparison result, wherein the standard value reflects the amount of fluid entering the normal flow path under the same conditions.

Another embodiment of the present application provides a detection device for a flow path, the flow path includes a first pipeline, a second pipeline, a valve body and a power device, one end of the first pipeline is used to connect to a fluid storage device, and the other end is connected to the valve body; one end of the second pipeline is connected to the valve body, and the other end is used to connect to a reaction device; a gas sensing device is provided on the second pipeline, and the gas sensing device sends a signal indicating that there is gas at a predetermined position of the second pipeline filled with fluid, and the valve body selectively connects the first pipeline and the second pipeline; the power device is used to provide power; the detection device includes:

A driving device, used to drive the first pipe so that one end of the first pipe connected to the fluid storage device is inserted into or removed from the fluid in the fluid storage device;

Controller; used for:

The control driving device drives one end of the first pipeline connected to the fluid storage device to insert the fluid in the fluid storage device, wherein the first pipeline is connected to the second pipeline through the valve body;

Controlling the power device to provide power so that the first pipeline draws fluid from the fluid storage device and allows the fluid to flow to the second pipeline, so that the flow path is filled with the fluid;

Control the driving device to drive the end of the first pipeline connected to the fluid storage device to leave the fluid in the fluid storage device, and control the power device to provide power so that the end of the first pipeline connected to the fluid storage device sucks gas and allows the gas to enter the first pipeline;

Control the driving device to drive one end of the first pipeline connected to the fluid storage device to insert into the fluid in the fluid storage device, and control the power device to provide power so that the fluid in the fluid storage device enters the first pipeline at a first specified speed and the gas enters the flow path filled with fluid;

receiving a signal from a gas sensing device;

determining the amount of fluid entering the flow path between the time when the fluid enters the first conduit and the time when a signal from the gas sensing device is received; and

The amount of fluid entering the flow path is compared with a standard value to obtain a comparison result, and it is determined whether or not there is an abnormality in the flow path based on the comparison result.

Yet another embodiment of the present application provides a computer-readable storage medium, on which a program is stored. The program can be executed by a processor to implement the above-mentioned method for detecting a flow path.

Implementing the embodiments of the present application will have the following beneficial effects:

The detection device or method in the above-mentioned embodiment of the present application allows gas to enter a flow path filled with fluid, then passes fluid to allow gas to enter the flow path, and receives a signal from a gas sensing device, determines the amount of fluid entering the flow path from the time the fluid enters the first pipe to the time the signal from the gas sensing device is received, and compares the amount of fluid entering the flow path with a standard value to obtain a comparison result, and determines whether there is an abnormality in the flow path based on the comparison result, which is convenient, fast and highly accurate.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will describe the embodiments or the prior art. The accompanying drawings required for the technical description are briefly introduced. Obviously, the accompanying drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying any creative work.

in:

FIG1 is a schematic diagram of the structure of the flow path of the present application;

FIG2 is a first structural schematic diagram of the detection device of the present application;

FIG3 is a second structural schematic diagram of the detection device of the present application;

FIG4 is a first flow chart of the method for detecting flow path of the present application;

FIG5 is a second flow chart of the method for detecting flow path of the present application;

FIG6 is a third flow chart of the method for detecting flow path of the present application;

FIG. 7 is a fourth flow chart of the method for detecting flow path of the present application.

Description of the reference numerals in the accompanying drawings:
First pipeline 11, valve body 12, second pipeline 13, gas sensing device or bubble sensor 14, fluid storage device
15. Power device 16. Controller 17. Driving device 18. Reaction device 19.

DETAILED DESCRIPTION

In order to enable ordinary technicians in the field to understand the present application, the present application will be further described below in conjunction with the accompanying drawings and specific implementation methods. It should be understood that the specific implementation methods described here are only used to explain the present application and are not used to limit the present application.

In this application, unless otherwise specified, the term "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

As shown in FIG. 1 , the flow path of the present application includes a first pipeline 11 , a valve body 12 , and a second pipeline 13 .

Among them, one end of the first pipeline 11 is used to connect the fluid storage device 15, and the other end of the first pipeline 11 is connected to the valve body 12. The fluid storage device 15 is used to store fluid, and the fluid is, for example, a solution for realizing biochemical reactions and signal detection. The valve body 12 is located between the first pipeline 11 and the second pipeline 13. When there are multiple first pipelines 11, each first pipeline 11 corresponds to a fluid storage device 15, and the valve body 12 can be used to selectively connect a first pipeline 11 with the second pipeline 13, so that the fluid in the corresponding fluid storage device 15 enters the reaction device 19 for biochemical reaction. The valve body 12 can be a three-way rotary valve or a multi-way rotary valve.

One end of the second pipeline 13 is connected to the valve body 12, and the other end is used to connect to the reaction device 19. The reaction device 19 is arranged downstream of the second pipeline 13 to provide a physical space for the biochemical reaction. A gas sensing device 14 is arranged at a predetermined position of the second pipeline 13, and the gas sensing device 14 is used to detect the gas passing through the predetermined position of the second pipeline 13.

As shown in FIG4 , the present application provides a method for detecting a flow path, comprising:

a) allowing gas to enter a flow path filled with fluid, including allowing gas to enter the first pipe 11, and allowing fluid to enter the first pipe 11 to allow gas to enter the flow path;

b) receiving a signal from the gas sensing device 14;

c) determining the amount of fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the gas sensing device 14 is received; and

In the above method, before the gas enters the flow path, the first pipeline 11 and the second pipeline 13 are connected through the valve body 12, and one end of the first pipeline 11 connected to the fluid storage device 15 is inserted into the fluid in the fluid storage device 15, and the power device is controlled to provide negative pressure power, so that the fluid enters the first pipeline 11 from the fluid storage device 15, flows through the valve body 12 and the second pipeline 13, until the fluid fills the entire flow path.

Specifically, when the gas enters the flow path filled with fluid, the end of the first pipe 11 connected to the fluid storage device 15 is separated from the fluid in the fluid storage device 15, and the end of the first pipe 11 connected to the fluid storage device 15 is placed in the air, and the power device 16 is controlled to provide negative pressure power, so that the end of the first pipe 11 connected to the fluid storage device 15 draws gas, so that the gas enters the first pipe 11, and then the end of the first pipe 11 connected to the fluid storage device 15 is inserted into the fluid of the fluid storage device 15, so that the fluid enters the first pipe 11 at a specified first speed to allow the gas to enter the flow path. When the gas reaches the predetermined position of the second pipe 13, the gas sensing device 14 detects the gas passing through the predetermined position and generates a sensing signal. At this time, the signal from the gas sensing device 14 is received.

In some embodiments, the timing is started when the fluid enters the first pipe 11 at a specified first speed, and the timing is ended when a signal from the gas sensing device 14 is received. The amount of the fluid entering the flow path between the time when the fluid enters the first pipe 11 and the time when the signal from the gas sensing device 14 is received is determined by the timing duration and the specified first speed. That is, the amount of the fluid entering the flow path is determined based on the specified first speed and the time required between the time when the fluid enters the first pipe 11 at the specified first speed and the time when the signal from the gas sensing device 14 is received.

In some embodiments, a) includes fluid entering the first conduit 11 at a specified first velocity, c) includes determining the time required from the start of a) to the completion of b), and determining the amount of fluid entering the flow path based on the required time and the specified first flow velocity.

As shown in FIG. 5 , in some embodiments, before executing step a), in order to determine the above standard value, the method for detecting the flow path further includes steps e) to h):

e) Fill the flow path with fluid to ensure that the flow path is a normal flow path;

f) allowing gas to enter the flow path filled with fluid, including allowing gas to enter the first pipe 11, and allowing fluid to enter the first pipe 11 to allow gas to enter the flow path;

g) receiving a signal from the gas sensing device 14;

h) Determine the amount of fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the gas sensing device 14 is received, and use the amount of fluid entering the flow path as a standard value.

Specifically, when executing e), the first pipeline 11 and the second pipeline 13 are connected through the valve body 12, and one end of the first pipeline 11 connected to the fluid storage device 15 is inserted into the fluid in the fluid storage device 15, and the power device 16 is controlled to provide negative pressure power, so that the fluid enters the first pipeline 11 from the fluid storage device 15, flows through the valve body 12 and the second pipeline 13, until the fluid fills the entire flow path. Check whether there is an abnormality in the flow path. If there is no abnormality, the flow path is determined to be a normal flow path, and the normal flow path can be used to obtain the above-mentioned standard value. On the contrary, if there is an abnormality in the flow path, the flow path is determined to be an abnormal flow path, and the abnormal flow path cannot be used to obtain the above-mentioned standard value.

After determining that the flow path is a normal flow path, execute f). Specifically, make the end of the first pipe 11 connected to the fluid storage device 15 leave the fluid storage device 15, and place the end of the first pipe 11 connected to the fluid storage device 15 in the air, control the power device 16 to provide negative pressure power, so that the end of the first pipe 11 connected to the fluid storage device 15 draws gas, so that the gas enters the first pipe 11, and then insert the end of the first pipe 11 connected to the fluid storage device 15 into the fluid storage device 15, so that the fluid enters the first pipe 11 at a specified second speed so that the gas enters the flow path, and when the gas reaches the predetermined position of the second pipe 13, the gas sensing device 14 detects the gas passing through the predetermined position and generates a sensing signal. At this time, the signal from the gas sensing device 14 is received.

In some embodiments, the timing is started when the fluid enters the first pipe 11 at the specified second speed, and the timing is ended when the signal from the gas sensing device 14 is received. The amount of the fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the gas sensing device 14 is received is determined by the timing duration and the specified second speed, and the amount of the fluid entering the flow path is used as a standard value. That is, the amount of the fluid entering the flow path is determined based on the specified second speed and the time required from the time the fluid enters the first pipe 11 at the specified second speed to the time the signal from the gas sensing device 14 is received, and the amount of the fluid entering the flow path is used as a standard value.

In some embodiments, f) includes the fluid entering the first pipe 11 at a specified second speed, h) includes determining the time required from the start of f) to the completion of g), and determining the amount of fluid entering the flow path based on the required time and the specified second flow rate, and using the amount of fluid entering the flow path as a standard value.

In the above method, when executing d), comparing the amount of fluid entering the flow path with a standard value to obtain a comparison result, and determining whether the flow path is abnormal based on the comparison result, including:

If there is a significant difference between the amount of fluid entering the flow path and the standard value, the flow path is judged to be abnormal; otherwise, the flow path is judged to be normal.

The significant difference between the amount of fluid entering the flow path and the standard value includes that the amount of fluid entering the flow path is significantly smaller than the standard value, or that the amount of fluid entering the flow path is significantly larger than the standard value.

If the amount of fluid entering the flow path is significantly less than the standard value, it is determined that there is an abnormality between the first pipeline 11 and the predetermined position of the flow path; if the amount of fluid entering the flow path is significantly greater than the standard value, it is determined that there is an abnormality between the predetermined position of the flow path and the reaction device 19. In this way, according to the difference between the amount of fluid entering the flow path and the standard value, the position where the abnormality of the flow path occurs can be preliminarily determined, thereby reducing the scope of manual investigation and improving work efficiency.

Furthermore, the amount of fluid entering the flow path is significantly less than the standard value, including the amount of fluid entering the flow path being 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%, 101%, 102%, 103%, 104%, 105%, 106%, 107%, 108%, 109%, 110%, 111%, 112%, 113%, 114%, 1 70%, 75%, 80%, 85%, 90%, 95% or values between any two of the above values. The amount of fluid entering the flow path is significantly greater than the standard value, including the amount of fluid entering the flow path exceeding the standard value by 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or values between any two of the above values.

In some embodiments, the abnormality of the flow path includes damage or leakage of the flow path. The damage or leakage of the flow path causes substances (e.g., gas) outside the flow path to enter the flow path in an abnormal way, or causes substances (e.g., liquid) in the flow path to flow out of the flow path in an abnormal way.

In some embodiments, the abnormality in the flow path includes causing a substance to enter the flow path through an abnormal path or causing a substance to flow out of the flow path through an abnormal path.

In some embodiments, as shown in Fig. 1, the first pipeline 11 includes a plurality of pipelines, each of which corresponds to a fluid storage device 15, and each of which stores the same or different fluids. The valve body 12 selectively connects the first pipeline 11 and the second pipeline 12, so that each of the first pipelines 11 in the flow path can be detected.

The present application allows gas to enter a flow path filled with fluid, then passes fluid to allow gas to enter the flow path, and receives a signal from the gas sensing device 14, determines the amount of fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the gas sensing device 14 is received, and compares the amount of fluid entering the flow path with a standard value to obtain a comparison result, and determines whether the flow path is abnormal based on the comparison result, which is convenient, fast and highly accurate. If there is an abnormality in the flow path, the method of the present application can also preliminarily determine the location of the abnormality in the flow path, thereby reducing the scope of manual investigation and improving work efficiency.

As shown in Figs. 1, 2 and 3, another embodiment of the present application provides a detection device for a flow path, wherein the flow path includes a first pipeline 11, a second pipeline 13, a valve body 12 and a power device 16. One end of the first pipeline 11 is used to connect to a fluid storage device 15, and the other end of the first pipeline 11 is connected to the valve body 12. The fluid storage device 15 is used to store fluid, and the fluid is, for example, a solution for realizing biochemical reactions and signal detection.

The valve body 12 is located between the first pipeline 11 and the second pipeline 13, and is used to selectively connect the first pipeline 11 and the second pipeline 13, so that the fluid in the fluid storage device 15 enters the reaction device 19 for biochemical reaction. The valve body 12 can be a three-way rotary valve or a multi-way rotary valve.

One end of the second pipeline 13 is connected to the valve body 12, and the other end is used to connect to the reaction device 19. The reaction device 19 is arranged downstream of the second pipeline 13 to provide a physical space for the biochemical reaction.

A gas sensing device 14 is disposed at a predetermined position of the second pipeline 13 , and the gas sensing device 14 is used to detect the gas passing through the predetermined position of the second pipeline 13 .

The power device 16 is disposed downstream of the reaction device 19 and connected to the reaction device 19 for providing negative pressure power.

The detection device includes a controller 17 and a driving device 18, and the controller 17 is connected to the driving device 18, the valve body 12, the power device 16 and the gas sensing device 14 respectively.

A driving device 18, used for driving the first pipe 11, so that one end of the first pipe 11 connected to the fluid storage device 15 is inserted into or removed from the fluid in the fluid storage device 15;

Controller 17; used for:

The control driving device 18 drives one end of the first pipeline 11 connected to the fluid storage device 15 to insert the fluid in the fluid storage device 15, wherein the first pipeline 11 is connected to the second pipeline 13 through the valve body 12;

Control the power device 16 to provide power so that the first pipeline 11 draws fluid from the fluid storage device 15 and makes the fluid flow to the second pipeline, so that the flow path is filled with fluid;

Control the driving device 18 to drive the end of the first pipeline 11 connected to the fluid storage device 15 to leave the fluid in the fluid storage device 15, and control the power device 16 to provide power so that the end of the first pipeline 11 connected to the fluid storage device 15 sucks gas and allows the gas to enter the first pipeline 11;

Control the driving device 18 to drive the end of the first pipeline 11 connected to the fluid storage device 15 to insert into the fluid in the fluid storage device 15, and control the power device 16 to provide power, so that the fluid in the fluid storage device 15 enters the first pipeline 11 at a first specified speed and the gas enters the flow path filled with fluid;

receiving a signal from a gas sensing device 14;

Determine the amount of fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the gas sensing device 14 is received; and

Specifically, during the detection process, the controller 17 controls the valve body 12 to connect the first pipeline 11 and the second pipeline 13, and controls the driving device 18 to drive one end of the first pipeline 11 connected to the fluid storage device 15 to insert the fluid in the fluid storage device 15, and then controls the power device 16 to provide negative pressure power so that the first pipeline 11 absorbs fluid from the fluid storage device 15 and makes the fluid flow to the second pipeline so that the fluid fills the flow path.

After the fluid fills the flow path, the controller 17 controls the driving device 18 to drive the end of the first pipeline 11 connected to the fluid storage device 15 to leave the fluid in the fluid storage device 15, and controls the power device 16 to provide power so that the end of the first pipeline 11 connected to the fluid storage device 15 can suck gas and allow the gas to enter the first pipeline 11.

After the gas enters the first pipeline 11, the controller 17 controls the driving device 18 to drive the end of the first pipeline 11 connected to the fluid storage device 15 to insert the fluid in the fluid storage device 15, and controls the power device 16 to provide negative pressure power, so that the fluid in the fluid storage device 15 enters the first pipeline 11 at a first specified speed and the gas enters the flow path filled with fluid. When the gas reaches the predetermined position of the second pipeline 13, the gas sensing device 14 detects the gas passing through the predetermined position and generates a sensing signal. The controller 17 receives the signal from the gas sensing device 14.

In some embodiments, when the fluid enters the first pipe 11 at a specified first speed, the controller 17 starts timing, and ends timing when receiving a signal from the gas sensing device 14. The controller 17 determines the amount of the fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the gas sensing device 14 is received, based on the timing duration and the specified first speed. That is, the controller 17 determines the amount of the fluid entering the flow path based on the specified first speed and the time required from the time the fluid enters the first pipe 11 at the specified first speed to the time the signal from the gas sensing device 14 is received.

In some embodiments, the fluid enters the first pipe 11 at a specified first speed, the controller 17 determines the time required between the fluid entering the first pipe 11 at the specified first speed and the controller 17 receiving the signal of the gas sensing device 14, and based on the time The amount of the fluid entering the flow path is determined at a specified first speed and a required time.

In some embodiments, in order to determine the standard value, the controller 17 is also used to control the valve body 12 to connect the first pipeline 11 and the second pipeline 13, and control the driving device 18 to drive the end of the first pipeline 11 connected to the fluid storage device 15 to be inserted into the fluid in the fluid storage device 15, and then control the power device 16 to provide negative pressure power, so that the fluid enters the first pipeline 11 from the fluid storage device 15, flows through the valve body 12 and the second pipeline 13, until the fluid fills the entire flow path. Check whether there is an abnormality in the flow path. If there is no abnormality, the flow path is determined to be a normal flow path, and the normal flow path can be used to obtain the above standard value. On the contrary, if there is an abnormality in the flow path, the flow path is determined to be an abnormal flow path, and the abnormal flow path cannot be used to obtain the above standard value.

After determining that the flow path is a normal flow path, the controller 17 controls the driving device 18 to drive the end of the first pipe 11 connected to the fluid storage device 15 to leave the fluid storage device 15, and controls the end of the first pipe 11 connected to the fluid storage device 15 to be placed in the air, and then controls the power device 16 to provide negative pressure power, so that the end of the first pipe 11 connected to the fluid storage device 15 draws gas into the first pipe 11, and then controls the end of the first pipe 11 connected to the fluid storage device 15 to be inserted into the fluid storage device 15, so that the fluid enters the first pipe 11 at a specified second speed to allow the gas to enter the flow path. When the gas reaches the predetermined position of the second pipe 13, the gas sensing device 14 detects the gas passing through the predetermined position and generates a sensing signal. The controller 17 receives the signal from the gas sensing device 14.

In some embodiments, when the fluid enters the first pipe 11 at the specified second speed, the controller 17 starts timing, and ends timing when receiving a signal from the gas sensing device 14. The controller 17 determines the amount of the fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the gas sensing device 14 is received by the timing duration and the specified second speed, and uses the amount of the fluid entering the flow path as a standard value. That is, the controller 17 determines the amount of the fluid entering the flow path based on the specified second speed and the time required from the time the fluid enters the first pipe 11 at the specified second speed to the time the signal from the gas sensing device 14 is received, and uses the amount of the fluid entering the flow path as a standard value.

In some embodiments, the fluid enters the first pipe 11 at a specified second speed, and the controller 17 determines the time required from the time when the fluid enters the first pipe 11 at the specified second speed to the time when the controller 17 receives the signal from the gas sensing device 14, and determines the amount of the fluid entering the flow path based on the specified second speed and the required time, and uses the amount of the fluid entering the flow path as a standard value.

The controller 17 compares the amount of fluid entering the flow path with the standard value, obtains a comparison result, and determines whether there is an abnormality in the flow path based on the comparison result.

If the amount of fluid entering the flow path is significantly different from the standard value, the controller 17 determines that there is an abnormality in the flow path; otherwise, the controller 17 determines that the flow path is a normal flow path.

If the amount of fluid entering the flow path is significantly less than the standard value, the controller 17 determines that there is an abnormality between the first pipeline 11 and the predetermined position of the flow path; if the amount of fluid entering the flow path is significantly greater than the standard value, the controller 17 determines that there is an abnormality between the predetermined position of the flow path and the reaction device 19. In this way, according to the specific difference between the fluid entering the flow path and the standard value, the position where the abnormality exists in the flow path can be preliminarily determined, thereby reducing the scope of manual investigation and improving work efficiency.

Further, the amount of fluid entering the flow path is significantly less than the standard value, including the amount of fluid entering the flow path is lower than the standard value by 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or a value between any two of the above values. The amount of fluid entering the flow path is significantly greater than the standard value, including the amount of fluid entering the flow path exceeds the standard value by 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or a value between any two of the above values.

The present application controls the detection of the flow path by cooperating with the controller 17, the valve body 12, the power device 16, the gas sensing device 14, and the driving device 18, thereby automating the flow path detection, which is convenient, fast, reliable, and accurate.

In some embodiments, the gas sensing device 14 is a bubble sensor 14, and the bubble sensor 14 is used to detect bubbles passing through a predetermined position of the second pipe 13, and the bubbles contain the above-mentioned gas.

As shown in FIG6 , in some embodiments, the present application provides a method for detecting a flow path, comprising:

a1) allowing air bubbles to enter a flow path filled with fluid, including allowing air bubbles to enter the first pipe 11, and allowing fluid to enter the first pipe 11 to allow air bubbles to enter the flow path;

b1) receiving a signal from the bubble sensor 14;

c1) determining the amount of fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the bubble sensor 14 is received; and

d1) Compare the amount of fluid entering the flow path with a standard value to obtain a comparison result, and determine whether there is an abnormality in the flow path based on the comparison result, wherein the standard value reflects the amount of fluid entering the normal flow path under the same conditions.

In the above method, before the bubbles enter the flow path, the first pipe 11 and the second pipe 13 are connected through the valve body 12, and one end of the first pipe 11 connected to the fluid storage device 15 is inserted into the fluid in the fluid storage device 15, and the power device 16 is controlled to provide negative pressure power, so that the fluid enters the first pipe 11 from the fluid storage device 15, flows through the valve body 12 and the second pipe 13, until the fluid fills the entire flow path.

Specifically, when the bubbles enter the flow path filled with fluid, the end of the first pipe 11 connected to the fluid storage device 15 is separated from the fluid storage device 15, and the end of the first pipe 11 connected to the fluid storage device 15 is placed in the air, and the power device 16 is controlled to provide negative pressure power, so that the end of the first pipe 11 connected to the fluid storage device 15 draws gas, so that the gas enters the first pipe 11 and forms a specified number of bubbles, such as a single bubble, in the first pipe 11; then the end of the first pipe 11 connected to the fluid storage device 15 is inserted into the fluid storage device 15, so that the fluid enters the first pipe 11 at a specified first speed to allow the bubbles to enter the flow path; when the bubbles reach a predetermined position of the second pipe 13, the bubble sensor 14 detects the bubbles passing through the predetermined position and generates an induction signal, and at this time the signal from the bubble sensor 14 is received.

In some embodiments, one end of the first pipe 11 connected to the fluid storage device 15 is connected to a reagent needle, and the reagent needle is used to extract gas or fluid in the fluid storage device 15. When the reagent needle is used to extract gas, the volume of gas extracted by the reagent needle can be controlled so that the gas forms a specified number of bubbles in the first pipe 11.

In some embodiments, the timing is started when the fluid enters the first pipe 11 at a specified first speed, and the timing is ended when a signal from the bubble sensor 14 is received. The timing duration and the specified first speed are used to determine the time since the fluid entered the first pipe. The amount of the fluid entering the flow path between the first pipe 11 and receiving the signal from the bubble sensor 14. That is, the amount of the fluid entering the flow path is determined based on the specified first speed and the time required between the fluid entering the first pipe 11 at the specified first speed and receiving the signal from the bubble sensor 14.

In some embodiments, a1) includes fluid entering the first conduit 11 at a specified first velocity, c1) includes determining the time required from the start of a1) to the completion of b1), and determining the amount of fluid entering the flow path based on the required time and the specified first flow velocity.

As shown in FIG. 7 , in some embodiments, before performing step a1), in order to determine the above standard value, the method for detecting the flow path further includes steps e1) to h1):

e1) Fill the flow path with fluid to ensure that the flow path is a normal flow path;

f1) allowing air bubbles to enter a flow path filled with fluid, including allowing air bubbles to enter the first pipe 11, and allowing fluid to enter the first pipe 11 to allow air bubbles to enter the flow path;

g1) receiving a signal from the bubble sensor 14;

h1) Determine the amount of fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the bubble sensor 14 is received, and use the amount of fluid entering the flow path as a standard value.

Specifically, when executing e1), the first pipeline 11 and the second pipeline 13 are connected through the valve body 12, and one end of the first pipeline 11 connected to the fluid storage device 15 is inserted into the fluid in the fluid storage device 15, and the power device 16 is controlled to provide negative pressure power, so that the fluid enters the first pipeline 11 from the fluid storage device 15, flows through the valve body 12 and the second pipeline 13, until the fluid fills the entire flow path. Check whether there is an abnormality in the flow path. If there is no abnormality, determine that the flow path is a normal flow path. The normal flow path can be used to obtain the above-mentioned standard value. On the contrary, if there is an abnormality in the flow path, determine that the flow path is an abnormal flow path, and the abnormal flow path cannot be used to obtain the above-mentioned standard value.

After determining that the flow path is a normal flow path, execute f1), that is: make the end of the first pipe 11 connected to the fluid storage device 15 leave the fluid storage device 15, and place the end of the first pipe 11 connected to the fluid storage device 15 in the air, control the power device 16 to provide negative pressure power, so that the end of the first pipe 11 connected to the fluid storage device 15 draws gas, so that the gas enters the first pipe 11 and forms a specified number of bubbles in the first pipe 11; then insert the end of the first pipe 11 connected to the fluid storage device 15 into the fluid storage device 15, so that the fluid enters the first pipe 11 at a specified second speed so that the bubbles enter the flow path; when the bubbles reach the predetermined position of the second pipe 13, the bubble sensor 14 detects the bubbles passing through the predetermined position and generates a sensing signal. At this time, the signal from the bubble sensor 14 is received.

In some embodiments, the timing is started when the fluid enters the first pipe 11 at the specified second speed, and the timing is ended when the signal from the bubble sensor 14 is received. The amount of the fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the bubble sensor 14 is received is determined by the timing duration and the specified second speed, and the amount of the fluid entering the flow path is used as the standard value. That is, the amount of the fluid entering the flow path is determined based on the specified second speed and the time required from the time the fluid enters the first pipe 11 at the specified second speed to the time the signal from the bubble sensor 14 is received, and the amount of the fluid entering the flow path is used as the standard value.

In some embodiments, f1) includes the fluid entering the first conduit 11 at a specified second velocity, h1) includes determining the time required from the start of f1) to the completion of g1), and determining the amount of fluid entering the flow path based on the required time and the specified second flow velocity, The amount of fluid entering the flow path is taken as the standard value.

In the above method, when executing step d1), the amount of fluid entering the flow path is compared with a standard value to obtain a comparison result, and based on the comparison result, it is determined whether the flow path is abnormal, including:

If the amount of fluid entering the flow path is significantly less than the standard value, it is determined that there is an abnormality between the first pipeline 11 and the predetermined position of the flow path; if the amount of fluid entering the flow path is significantly greater than the standard value, it is determined that there is an abnormality between the predetermined position of the flow path and the reaction device 19. In this way, according to the specific difference between the fluid entering the flow path and the standard value, the position where the abnormality of the flow path occurs can be preliminarily determined, thereby reducing the scope of manual investigation and improving work efficiency.

In some embodiments, when there is an abnormality between the first pipe 11 and the preset position, the bubble sensor 14 detects that the number of bubbles is greater than a specified number and sends a corresponding signal.

The present application allows bubbles to enter a flow path filled with fluid, then introduces fluid to allow bubbles to enter the flow path, and receives a signal from the bubble sensor 14, determines the amount of fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the bubble sensor 14 is received, and compares the amount of fluid entering the flow path with a standard value to obtain a comparison result, and determines whether the flow path is abnormal based on the comparison result, which is convenient, fast and more accurate. If there is an abnormality in the flow path, the method of the present application can also preliminarily determine the location of the abnormality in the flow path, thereby narrowing the scope of manual investigation and improving work efficiency.

Another embodiment of the present application provides a detection device for a flow path, the flow path includes a first pipeline 11, a second pipeline 13, a valve body 12 and a power device 16. One end of the first pipeline 11 is used to connect to a fluid storage device 15, and the other end of the first pipeline 11 is connected to the valve body 12. The fluid storage device 15 is used to store fluid, and the fluid is, for example, a solution for realizing biochemical reactions and signal detection.

A bubble sensor 14 is provided at a predetermined position of the second pipeline 13. The bubble sensor 14 is used to detect the bubble passing through the second pipeline. 13 Bubbles at predetermined locations.

The detection device includes a controller 17 and a driving device 18, and the controller 17 is connected to the driving device 18, the valve body 12, the power device 16 and the bubble sensor 14 respectively.

Controller 17; used for:

Control the driving device 18 to drive one end of the first pipe 11 connected to the fluid storage device 15 to insert the fluid in the fluid storage device 15;

Control the power device 16 to provide power so that the first pipeline 11 draws fluid from the fluid storage device 15 and makes the fluid flow to the second pipeline 13, so that the flow path is filled with fluid;

Control the driving device 18 to drive the end of the first pipe 11 connected to the fluid storage device 15 to leave the fluid in the fluid storage device 15, and control the power device 16 to provide power so that the end of the first pipe 11 connected to the fluid storage device 15 sucks gas, and the gas enters the first pipe 11 and forms a specified number of bubbles in the first pipe 11;

Control the driving device 18 to drive the end of the first pipe 11 connected to the fluid storage device 15 to insert into the fluid in the fluid storage device 15, and control the power device 16 to provide power so that the fluid in the fluid storage device 15 enters the first pipe 11 at a first specified speed and bubbles enter the flow path filled with fluid;

Receiving a signal from the bubble sensor 14;

Determine the amount of fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the bubble sensor 14 is received; and

Specifically, during the detection process, the controller 17 controls the valve body 12 to connect the first pipeline 11 and the second pipeline 13, and controls the driving device 18 to drive one end of the first pipeline 11 connected to the fluid storage device 15 to insert the fluid in the fluid storage device 15, and then controls the power device 16 to provide negative pressure power so that the first pipeline 11 absorbs fluid from the fluid storage device 15 and makes the fluid flow to the second pipeline 13, so that the fluid fills the flow path.

After the fluid fills the flow path, the controller 17 controls the driving device 18 to drive the end of the first pipe 11 connected to the fluid storage device 15 to leave the fluid in the fluid storage device 15, and controls the power device 16 to provide negative pressure power so that the end of the first pipe 11 connected to the fluid storage device 15 sucks gas, and allows the gas to enter the first pipe 11 and form a specified number of bubbles, such as a single bubble, in the first pipe 11.

After the bubble enters the first pipe 11, the controller 17 controls the driving device 18 to drive the end of the first pipe 11 connected to the fluid storage device 15 to insert the fluid in the fluid storage device 15, and controls the power device 16 to provide negative pressure power, so that the fluid in the fluid storage device 15 enters the first pipe 11 at a first specified speed and the bubble enters the flow path filled with fluid. When the bubble reaches the predetermined position of the second pipe 13, the bubble sensor 14 detects the bubble passing through the predetermined position and The controller 17 receives the signal from the bubble sensor 14 .

In some embodiments, when the fluid enters the first pipe 11 at a specified first speed, the controller 17 starts timing, and ends timing when receiving a signal from the bubble sensor 14. The controller 17 determines the amount of the fluid that enters the flow path from the time the fluid enters the first pipe 11 to the time the signal from the bubble sensor 14 is received, based on the timing duration and the specified first speed. That is, the controller 17 determines the amount of the fluid that enters the flow path based on the specified first speed and the time required from the time the fluid enters the first pipe 11 at the specified first speed to the time the signal from the bubble sensor 14 is received.

In some embodiments, the fluid enters the first pipe 11 at a specified first speed, the controller 17 determines the time required between the fluid entering the first pipe 11 at the specified first speed and the controller 17 receiving the signal from the bubble sensor 14, and determines the amount of the fluid entering the flow path based on the specified first speed and the required time.

In some embodiments, in order to determine the standard value, the controller 17 is also used to control the valve body 12 to connect the first pipeline 11 and the second pipeline 13, and control the driving device 18 to drive the end of the first pipeline 11 connected to the fluid storage device 15 to be inserted into the fluid in the fluid storage device 15, and then control the power device 16 to provide negative pressure power, so that the fluid enters the first pipeline 11 from the fluid storage device 15, flows through the valve body 12 and the second pipeline 13, until the fluid fills the entire flow path. Check whether there is an abnormality in the flow path. If there is no abnormality, determine that the flow path is a normal flow path. The normal flow path can be used to obtain the above-mentioned standard value. On the contrary, if there is an abnormality in the flow path, determine that the flow path is an abnormal flow path, and the abnormal flow path cannot be used to obtain the above-mentioned standard value.

After determining that the flow path is a normal flow path, the controller 17 controls the driving device 18 to drive the end of the first pipe 11 connected to the fluid storage device 15 to leave the fluid storage device 15, and controls the end of the first pipe 11 connected to the fluid storage device 15 to be placed in the air, and then controls the power device 16 to provide negative pressure power, so that the end of the first pipe 11 connected to the fluid storage device 15 draws gas, so that the gas enters the first pipe 11 and forms a specified number of bubbles in the first pipe 11, and then controls the end of the first pipe 11 connected to the fluid storage device 15 to be inserted into the fluid storage device 15, so that the fluid enters the first pipe 11 at a specified second speed so that the bubbles enter the flow path, and when the bubbles reach the predetermined position of the second pipe 13, the bubble sensor 14 detects the bubbles passing through the predetermined position and generates a sensing signal. The controller 17 receives the signal from the bubble sensor 14.

In some embodiments, when the fluid enters the first pipe 11 at the specified second speed, the controller starts timing, and ends timing when receiving a signal from the bubble sensor 14. The controller 17 determines the amount of the fluid entering the flow path from the time the fluid enters the first pipe 11 to the time the signal from the bubble sensor 14 is received through the timing duration and the specified second speed, and uses the amount of the fluid entering the flow path as a standard value. That is, the controller 17 determines the amount of the fluid entering the flow path based on the specified second speed and the time required from the time the fluid enters the first pipe 11 at the specified second speed to the time the signal from the bubble sensor 14 is received, and uses the amount of the fluid entering the flow path as a standard value.

In some embodiments, the fluid enters the first pipe 11 at a specified second speed, and the controller 17 determines the time required between the fluid entering the first pipe 11 at the specified second speed and the controller 17 receiving the signal from the bubble sensor 14, and determines the amount of the fluid entering the flow path based on the specified second speed and the required time, and uses the amount of the fluid entering the flow path as a standard value.

The controller 17 compares the amount of fluid entering the flow path with the standard value, obtains a comparison result, and determines based on the comparison result. Check whether there are any abnormalities in the flow path. Including:

The present application controls the detection of the flow path by cooperating with the controller 17, the valve body 12, the power device, the bubble sensor 14, and the drive device 18, thereby automating the flow path detection, which is convenient, fast, reliable, and accurate.

The above are only some implementation methods of the present application. For ordinary technicians in this field, several variations and improvements can be made without departing from the creative concept of the present application, which all fall within the scope of protection of the present application.

Claims

A method for detecting a flow path, characterized in that the flow path comprises a first pipeline, a second pipeline and a valve body, the valve body selectively connects the first pipeline and the second pipeline, a gas sensing device is provided on the second pipeline, and the gas sensing device sends a signal indicating that gas exists at a predetermined position of the second pipeline filled with fluid, the method comprising:

a) allowing gas to enter a flow path filled with fluid, comprising allowing the gas to enter the first pipe, and allowing the fluid to enter the first pipe to allow the gas to enter the flow path;

b) receiving a signal from the gas sensing device;

c) determining the amount of fluid entering the flow path from the time the fluid enters the first conduit to the time the signal from the gas sensing device is received; and

d) comparing the amount of the fluid entering the flow path with a standard value to obtain a comparison result, and determining whether there is an abnormality in the flow path based on the comparison result, wherein the standard value reflects the amount of the fluid entering a normal flow path under the same conditions.
The method according to claim 1, further comprising:

The first pipeline and the second pipeline are connected to allow fluid to enter the first pipeline and flow through the valve body and the second pipeline, so that the flow path is in a state of being filled with fluid.
The method according to claim 1 or 2, characterized in that a) includes allowing the fluid to enter the first conduit at a specified first velocity and starting a timer.
The method of claim 3, wherein b) includes receiving a signal from the gas sensing device and ending timing.
The method of claim 4, wherein c) comprises determining the amount of the fluid entering the flow path based on the timed duration and the specified first speed.
The method according to any one of claims 1 to 5, characterized in that a) comprises causing the fluid to enter the first conduit at a specified first velocity.
The method of claim 6, wherein c) comprises determining a time required from the start of a) to the completion of b), and determining an amount of the fluid entering the flow path based on the time and the specified first speed.
The method according to any one of claims 1 to 7, characterized in that d) comprises:

If there is a significant difference between the amount of the fluid entering the flow path and the standard value, it is determined that there is an abnormality in the flow path; otherwise, it is determined that the flow path is a normal flow path.
The method according to claim 8, characterized in that the second pipeline connects the valve body and the reaction device, and d) comprises:

If the amount of the fluid entering the flow path is significantly less than the standard value, it is determined that there is an abnormality between the first pipeline of the flow path and the predetermined position;

If the amount of the fluid entering the flow path is significantly greater than the standard value, it is determined that an abnormality exists between the predetermined position of the flow path and the reaction device.
The method according to claim 9, wherein the abnormality of the flow path includes damage to the flow path or leakage.
The method according to claim 9, wherein the abnormality in the flow path includes causing a substance to enter the flow path in an abnormal way or causing a substance to flow out of the flow path in an abnormal way.
The method according to any one of claims 1 to 11, characterized in that, in order to determine the standard value, the method further comprises e) to h):

e) filling the flow path with fluid and determining that the flow path is a normal flow path;

f) allowing gas to enter the flow path filled with fluid, comprising allowing the gas to enter the first pipe, and allowing the fluid to enter the first pipe to allow the gas to enter the flow path;

g) receiving a signal from the gas sensing device;

h) determining the amount of the fluid entering the flow path from the time the fluid enters the first pipe to the time the signal from the gas sensing device is received, and using the amount of the fluid entering the flow path as the standard value.
The method according to claim 12, characterized in that e) includes connecting the first pipeline and the second pipeline, allowing the fluid to enter the first pipeline and flow through the valve body and the second pipeline, so that the flow path is in a state of being filled with fluid.
The method of claim 12, wherein f) includes causing the fluid to enter the first conduit at a specified second velocity and starting a timer.
The method of claim 14, wherein g) includes receiving a signal from the gas sensing device and terminating timing.
The method of claim 15, wherein h) comprises determining the amount of the fluid entering the flow path based on the timing duration and the specified second speed, and using the amount of the fluid entering the flow path as a standard value.
The method according to any one of claims 12 to 15, characterized in that f) comprises causing the fluid to enter the first conduit at a specified second velocity.
The method of claim 17, wherein h) includes determining the time required from the start of f) to the completion of g), and determining the amount of the fluid entering the flow path based on the time and the specified second speed, and using the amount of the fluid entering the flow path as a standard value.
A detection device for a flow path, characterized in that the flow path includes a first pipeline, a second pipeline, a valve body and a power device, one end of the first pipeline is used to connect to a fluid storage device, and the other end is connected to the valve body; one end of the second pipeline is connected to the valve body, and the other end is used to connect to a reaction device; a gas sensing device is provided on the second pipeline, and the gas sensing device sends a signal indicating that there is gas at a predetermined position of the second pipeline filled with fluid, and the valve body selectively connects the first pipeline and the second pipeline; the power device is used to provide power; the detection device includes:

A driving device, used for driving the first pipe so that one end of the first pipe connected to the fluid storage device is inserted into or removed from the fluid in the fluid storage device;

Controller; used for:

Control the driving device to drive the first pipeline to connect one end of the fluid storage device and insert the fluid in the fluid storage device, wherein the first pipeline is connected to the second pipeline through the valve body;

Controlling the power device to provide power so that the first pipeline draws fluid from the fluid storage device and makes the fluid flow to the second flow path, so that the flow path is filled with the fluid;

Control the driving device to drive the end of the first pipeline connected to the fluid storage device to leave the fluid in the fluid storage device, and control the power device to provide power so that the end of the first pipeline connected to the fluid storage device sucks gas and allows the gas to enter the first pipeline;

Control the driving device to drive the end of the first pipe connected to the fluid storage device to insert into the fluid in the fluid storage device, and control the power device to provide power so that the fluid in the fluid storage device enters the first pipe at a first specified speed and the gas enters the flow path filled with fluid;

receiving a signal from the gas sensing device;

determining the amount of fluid entering the flow path from the time the fluid enters the first conduit to the time the signal from the gas sensing device is received; and

The amount of the fluid entering the flow path is compared with a standard value to obtain a comparison result, and it is determined whether the flow path has an abnormality based on the comparison result.
The detection device according to claim 19, characterized in that when the fluid enters the first pipe at the specified first speed, the controller starts timing.
The detection device according to claim 20, characterized in that the controller ends the timing when receiving a signal from the gas sensing device.
The detection device as described in claim 21 is characterized in that the controller determines the amount of the fluid entering the flow path based on the timing duration and the specified first speed.
The detection device according to claim 19 or 20, characterized in that the controller determines the time required between the fluid entering the first pipe at the specified first speed and receiving the signal from the gas sensing device, and determines the amount of the fluid entering the flow path based on the time and the specified first flow rate.
The detection device according to any one of claims 19 to 23, characterized in that the controller compares the amount of the fluid entering the flow path with a standard value to obtain a comparison result, and determines whether there is an abnormality in the flow path based on the comparison result, comprising:

If the amount of the fluid entering the flow path is significantly less than the standard value, it is determined that there is an abnormality between the first pipeline of the flow path and the predetermined position;

If the amount of the fluid entering the flow path is significantly greater than the standard value, it is determined that an abnormality exists between the predetermined position of the flow path and the reaction device.
The detection device according to any one of claims 19 to 24, characterized in that, in order to determine the standard value, the controller is further used to:

Control the driving device to drive the end of the first pipe connected to the fluid storage device to insert into the fluid storage device. a fluid in the device, wherein the first conduit is in communication with the second conduit through the valve body;

Controlling the power device to provide power so that the first pipeline draws fluid from the fluid storage device and makes the fluid flow to the second flow path, so that the flow path is filled with the fluid;

Control the driving device to drive the end of the first pipeline connected to the fluid storage device to leave the fluid of the fluid storage device, and control the power device to provide power so that the end of the first pipeline connected to the fluid storage device sucks gas and allows the gas to enter the first pipeline;

Control the driving device to drive the end of the first pipe connected to the fluid storage device to insert into the fluid in the fluid storage device, and control the power device to provide power so that the fluid in the fluid storage device enters the first pipe at a first specified speed and the gas enters the flow path filled with fluid;

receiving a signal from the gas sensing device;

The amount of the fluid entering the flow path from the time the fluid enters the first pipe to the time the signal from the gas sensing device is received is determined, and the amount of the fluid entering the flow path is used as the standard value.
The detection device according to claim 25, characterized in that when the fluid enters the first pipe at the specified second speed, the controller starts timing.
The detection device according to claim 26, characterized in that the controller ends the timing when receiving a signal from the gas sensing device.
The detection device as described in claim 26 is characterized in that the controller determines the amount of the fluid entering the flow path based on the timing duration and the specified second speed, and uses the amount of the fluid entering the flow path as the standard value.
The detection device as claimed in claim 26 is characterized in that the controller determines the time required between the fluid entering the first pipe at the specified second speed and receiving the signal from the gas sensing device, and determines the amount of the fluid entering the flow path based on the time and the specified second flow rate, and uses the amount of the fluid entering the flow path as the standard value.
A computer-readable storage medium, characterized in that a program is stored on the medium, and the program can be executed by a processor to implement the method according to any one of claims 1 to 19.