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WO2024213150A1 - 一种孵育支架、孵育装置、孵育箱、染色设备及染色方法 - Google Patents

一种孵育支架、孵育装置、孵育箱、染色设备及染色方法 Download PDF

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Publication number
WO2024213150A1
WO2024213150A1 PCT/CN2024/087638 CN2024087638W WO2024213150A1 WO 2024213150 A1 WO2024213150 A1 WO 2024213150A1 CN 2024087638 W CN2024087638 W CN 2024087638W WO 2024213150 A1 WO2024213150 A1 WO 2024213150A1
Authority
WO
WIPO (PCT)
Prior art keywords
incubation
cover plate
plate
support
slide
Prior art date
Application number
PCT/CN2024/087638
Other languages
English (en)
French (fr)
Inventor
郭金灿
史健
宋启淼
庄吉祥
林建辉
马红亮
康宝金
林清华
Original Assignee
厦门通灵生物医药科技股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN202310391619.9A external-priority patent/CN118857878A/zh
Priority claimed from CN202310391610.8A external-priority patent/CN118857877A/zh
Priority claimed from CN202310391486.5A external-priority patent/CN118857876A/zh
Priority claimed from CN202310391626.9A external-priority patent/CN118857879A/zh
Application filed by 厦门通灵生物医药科技股份有限公司 filed Critical 厦门通灵生物医药科技股份有限公司
Publication of WO2024213150A1 publication Critical patent/WO2024213150A1/zh

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples

Definitions

  • the disclosed embodiments relate to the field of biological detection technology, and in particular to an incubation support, an incubation device, an incubation box, a staining device, and a staining method.
  • static incubation is mostly used for staining or detection, that is, the reagent is added to the slide specimen and incubated for a certain period of time, and then washed and the next step reagent is added.
  • the detection object in the reagent combines with the detection object in the specimen at a slow speed, the reagent concentration and distribution are uneven, and local bubbles gather, which affects the detection efficiency, sensitivity and accuracy.
  • the purpose of the present disclosure is to provide an incubation bracket, an incubation device, an incubation box, a staining device and a staining method, which at least to a certain extent overcome the problem that the detection efficiency and accuracy are affected by the long reaction time between the reagent and the specimen during static incubation in the related art.
  • an incubation bracket capable of loading a slide
  • the incubation bracket comprising a bracket assembly and a base plate and a cover plate obliquely arranged on the bracket assembly, the base plate being used to load the slide, the slide being located between the base plate and the cover plate; the cover plate slides in contact with the slide, or the cover plate opens and closes relative to the slide to mix the reagent between the cover plate and the slide.
  • an incubation device comprising a base, a tilting mechanism and the above-mentioned incubation support, wherein the tilting mechanism is arranged on the base, and the tilting mechanism is drivingly connected to the movable support assembly, and the tilting mechanism is used to drive the support assembly to swing so as to adjust the inclination angle of the bottom plate, the cover plate and the carrier relative to the horizontal plane.
  • an incubation box comprising a shell mechanism, a driving mechanism and an incubation mechanism, wherein the shell mechanism is provided with an installation cavity;
  • the driving mechanism comprises a driving member and a driven member, wherein the driving member is drivingly connected to the driven member;
  • the incubation mechanism comprises a supporting assembly and the above-mentioned incubation bracket, wherein the supporting assembly is connected to the inner wall of the installation cavity;
  • the supporting assembly comprises an incubation pot provided with an incubation trough, wherein the incubation bracket is arranged in the incubation trough;
  • the cover plate is drivingly connected to the transmission member;
  • the driving member can drive the driven member to move, so as to drive the cover plate to move relative to the carrier.
  • a staining device comprising a machine platform, an incubator as described above, and a tilting mechanism, wherein the tilting mechanism is arranged on the machine platform and is drivingly connected to the incubator, and can drive the incubator to swing downward so that the inclination of the bracket assembly, the cover plate, the bottom plate and the carrier relative to the horizontal plane becomes smaller.
  • a staining method is provided, which is applied to the staining equipment as described above, and the method comprises: adding liquid to the gap between the cover plate and the slide; the tilting mechanism drives the incubator to swing downward to an incubation state, and the tilting mechanism stops driving; the cover plate moves relative to the slide to mix the liquid between the cover plate and the slide; the tilting mechanism drives the incubator to swing upward, and when the incubator is swung to be parallel to a horizontal plane, the tilting mechanism stops driving.
  • FIG. 1 is a schematic structural diagram of an incubation support in Embodiment 1 at a first viewing angle.
  • FIG. 2 is a schematic structural diagram of the incubation support in FIG. 1 at a second viewing angle.
  • FIG3 is a schematic diagram of the cross-sectional structure of the incubation support in FIG2 in the C-C direction.
  • Figure 4 is a schematic diagram of the cross-sectional structure of another incubation support in Example 1 in the C-C direction.
  • FIG5 is a schematic diagram of the cross-sectional structure of the incubation support in FIG2 in the D-D direction.
  • FIG. 6 is a schematic diagram of the structure of the cover plate and the carrier in FIG. 1 during the process of bonding or separating.
  • FIG. 7 is a schematic structural diagram of the cover plate and the carrier sheet in FIG. 1 when they are separated.
  • FIG. 8 is a schematic structural diagram of the cover plate and the carrier sheet in FIG. 1 when they are bonded together.
  • FIG. 9 is a schematic structural diagram of the left side plate and the left driving member in FIG. 1 .
  • FIG. 10 is a schematic structural diagram of the cover plate in FIG. 1 .
  • FIG. 11 is a schematic structural diagram of the first base plate in FIG. 1 .
  • FIG. 12 is a schematic structural diagram of the second base plate in FIG. 1 .
  • FIG. 13 is a schematic structural diagram of the position limiting platform in FIG. 1 .
  • FIG. 14 is a schematic structural diagram of the incubation support in the second embodiment at a first viewing angle.
  • FIG. 15 is a schematic structural diagram of another incubation support in the second embodiment.
  • FIG. 16 is a schematic diagram of the structure of the straight plate and the carrier during the bonding or separation process in FIG. 14 .
  • FIG. 17 is a schematic diagram of the structure of the straight plate and the carrier sheet in FIG. 14 when they are bonded together.
  • FIG. 18 is a schematic diagram of the structure when the straight plate and the carrier are separated in FIG. 14 .
  • FIG. 19 is a schematic diagram of the structure of the upper portion of the arc-shaped plate in FIG. 14 during the process of bonding or separating with the carrier.
  • FIG. 20 is a schematic diagram of the structure when the upper portion of the curved plate in FIG. 14 is bonded to the carrier.
  • FIG. 21 is a schematic diagram of the structure when the arc plate and the carrier are separated in FIG. 14 .
  • FIG. 22 is a schematic structural diagram of the cover plate in FIG. 14 .
  • FIG. 23 is a schematic diagram of the structure of the bottom plate in FIG. 14 .
  • FIG. 24 is another schematic diagram of the structure of the bottom plate in FIG. 14 .
  • FIG. 25 is a schematic diagram of the left side plate and the left driving member in FIG. 14 .
  • FIG. 26 shows a schematic structural diagram of an incubation support in Example 3.
  • FIG. 27 shows a schematic structural diagram of another incubation support in Example 3.
  • FIG. 28 is a schematic structural diagram of another incubation support in the third embodiment.
  • FIG. 29 shows a schematic structural diagram of another incubation support in the third embodiment.
  • FIG30 is a schematic diagram showing the structure of an incubation support in Example 4.
  • FIG. 31 is a schematic structural diagram of the bottom plate in FIG. 30 .
  • FIG. 32 is a schematic diagram of the structure of the cover plate and the carrier during the bonding or separation process in FIG. 30 .
  • FIG. 33 is a schematic diagram of the structure when the cover plate and the carrier sheet in FIG. 30 are bonded together.
  • FIG34 is a schematic diagram showing the structure of the incubator in the fifth embodiment.
  • Figure 35 is a schematic structural diagram of the incubation support separation of the incubation mechanism in Figure 34.
  • FIG. 36 is a schematic structural diagram of the shell mechanism and the driving mechanism in FIG. 34 .
  • Figure 37 is a cross-sectional schematic diagram of the incubator in Figure 34 at a first viewing angle.
  • FIG. 38 is a schematic diagram of the exploded structure of the support assembly of the incubation mechanism in FIG. 34 .
  • Figure 39 is a schematic diagram of the structure of the support assembly and incubation support in Figure 34.
  • FIG. 40 is a schematic diagram of the structure of the incubation support in FIG. 34 .
  • FIG. 41 is a schematic diagram of the first structure of the incubator in FIG. 34 after removing the rear plate.
  • FIG42 is a schematic cross-sectional view of the incubator in FIG34 at a second viewing angle.
  • FIG. 43 is a schematic diagram of a second structure of the incubator in FIG. 34 after removing the rear plate.
  • FIG. 44 shows a schematic structural diagram of the dyeing equipment of Example 6.
  • FIG. 45 is a schematic diagram showing the cross-sectional structure of the incubator and the tilting mechanism in FIG. 44 in the initial state.
  • FIG. 46 is a schematic diagram showing the cross-sectional structure of the incubator and the tilting mechanism in FIG. 44 in the incubation state.
  • Figure 47 is a schematic diagram of the cross-sectional structure of the tilting mechanism and operating platform in Figure 44.
  • Figure 48 shows a schematic structural diagram of another dyeing device in Example 6.
  • FIG. 49 is a schematic diagram showing the cross-sectional structure of the incubator in FIG. 48 .
  • Figure 50 shows a schematic structural diagram of an incubation device in Example 7.
  • Figure 51 is a schematic diagram of the exploded structure of the incubation device in Figure 50.
  • FIG52 shows a schematic structural diagram of another incubation device in Example 7.
  • FIG53 shows a schematic structural diagram of yet another incubation device in Example 7.
  • FIG. 54 shows a schematic structural diagram of an incubation support in an embodiment of the present disclosure.
  • Figure 55 is a schematic diagram of a partially exploded structure of an incubation support in Figure 54.
  • Figure 56 is a schematic diagram of the cross-sectional structure of an incubation support in Figure 54 at a first angle.
  • Figure 57 is a schematic diagram of the cross-sectional structure of an incubation support in Figure 54 at a second angle.
  • Figure 58 is a schematic diagram of the structure of an incubation bracket in Figure 54 without one side plate.
  • Figure 59 is a schematic diagram of the structure of the base plate, carrier and curved plate in Figure 58.
  • Figure 60 is a schematic diagram of the structure of the curved plate in Figure 58.
  • Figure 61 is a schematic diagram of the partially exploded structure of another incubation support in Figure 54.
  • Figure 62 is a schematic diagram of the cross-sectional structure of another incubation support in Figure 54 at a first angle.
  • Figure 63 is a schematic diagram of the cross-sectional structure of another incubation support in Figure 54 at a second angle.
  • Figure 64 is a schematic diagram of the structure of another incubation support in Figure 54 without one side plate.
  • Figure 65 is a schematic diagram of the structure of the base plate, carrier and curved plate in Figure 64.
  • Figure 66 is a schematic diagram of the structure of the curved plate in Figure 64.
  • the present embodiment provides an incubation support 1, which may include a support assembly 100, a pair of pushers 400, and a bottom plate 200 and a cover plate 300 obliquely arranged on the support assembly 100.
  • the support assembly 100 includes a pair of side plates, the bottom plate 200 is used to load a slide 600, and the upper surface of the slide 600 is used to set a sample and/or a reagent.
  • the cover plate 300 is arranged above the bottom plate 200, and the slide 600 is located between the bottom plate 200 and the cover plate 300.
  • a plurality of base plates 200 may be provided on the support assembly 100, and each base plate 200 may be loaded with one or more slides 600. This facilitates the simultaneous staining and detection of multiple slide specimens, effectively improving the processing efficiency.
  • Each base plate 200 is correspondingly provided with a cover plate 300.
  • a pair of pushers 400 are slidably connected to a pair of side plates, and the bottom plate 200 and the cover plate 300 are connected between the pair of side plates.
  • the bottom plate 200 is connected to the side plates in a fixed or detachable manner, and the cover plate 300 is provided with protrusions 301 on both sides, and the two protrusions 301 are detachably connected to the pair of pushers 400.
  • the side plate is provided with a limit groove 103, and the limit groove 103 is provided with a first support surface 104.
  • the pusher 400 is provided with a movable groove 404, and the movable groove 404 is provided with a second support surface 405.
  • the first support surface 104 and the second support surface 405 are arranged crosswise, and the protrusion 301 can be supported at the intersection of the first support surface 104 and the second support surface 405.
  • the limit groove 103 is used to limit the distance of the protrusion 301 to reciprocate along the bottom of the first support surface 104 to the top of the first support surface 104.
  • the protrusion 301 is arranged on the upper part of the cover plate 300.
  • the bracket assembly 100 includes a first connecting portion 107 and a second connecting portion 108 respectively connected between a pair of side panels, and the first connecting portion 107 and the second connecting portion 108 are respectively arranged at the front end and the end of the side panel.
  • the first connecting portion 107 and the second connecting portion 108 can be a connecting plate, a connecting rod, etc., for stabilizing the side panel.
  • the side panel may include a left side panel 101 and a right side panel 102 that are symmetrically arranged.
  • the limiting grooves 103 on the left side panel 101 and the right side panel 102 can be arranged as a straight groove.
  • the first supporting surface 104 is arranged to cross the upper surface of the loaded carrier 600.
  • the pusher 400 may be a slide bar or a slide plate.
  • the pusher 400 includes a left pusher 401 and a right pusher 402 that are symmetrically arranged.
  • the left pusher 401 is slidably connected to the left side plate 101
  • the right pusher 402 is slidably connected to the right side plate 102.
  • a slide groove 105 is provided on both the left side plate 101 and the right side plate 102.
  • the left pusher 401 is arranged in the slide groove 105 on the left side plate 101.
  • the right pusher 402 is arranged in the slide groove 105 on the right side plate 102, and the pusher 400 can reciprocate in the slide groove 105.
  • the slide groove 105 can be provided on the inner side of the side plate and extend from one end to the other end of the side plate.
  • the slide groove 105 is adapted to the push member 400, and the left push member 401 and the right push member 402 can synchronously reciprocate in the slide groove 105 in the first direction A or the second direction B.
  • the slide groove 105 can also be provided on the outer side of the side plate.
  • a guide groove 106 is also provided on the left side plate 101 and the right side plate 102.
  • the opening of the guide groove 106 extends from the top of the side plate to the limiting groove 103.
  • the guide groove 106 is connected to the limiting groove 103.
  • the protrusion 301 can enter or exit the limiting groove 103 from the guide groove 106, which is convenient for taking and placing the cover plate 300.
  • the left pusher 401 and the right pusher 402 are both provided with movable grooves 404, and the movable grooves 404 on both sides are symmetrical.
  • the movable groove 404 is a space similar to a trapezoid, and the protrusion 301 can move in the space.
  • the limiting groove 103 is in a straight line shape, and the two parallel inner walls of the limiting groove 103 limit the movement of the protrusion 301, so that the protrusion 301 moves in a straight line in the groove and always contacts the first support surface 104.
  • the protrusion 301 can move freely in the movable groove 404 and can be separated from the second support surface 405.
  • the lower surface of the cover plate 300 is provided with a second isolating portion 302 and a support platform 303, and the support platform 303 protrudes from the lower surface of the cover plate 300.
  • the second isolating portion 302 is used to separate the reagents on two adjacent slides 600.
  • the cover plate 300 is of a specification that can cover three slides 600, and the cover plate 300 is provided with two second isolating portions 302, and the second isolating portions 302 can be rectangular grooves.
  • the three contact surfaces where the cover plate 300 and the three slides 600 are attached are separated by the second isolating portion 302 to prevent the reagents on the adjacent slides 600 from flowing to each other and causing cross contamination.
  • the height of the support platform 303 protruding from the lower surface of the cover plate 300 is 0.01-2mm.
  • the four corners of each contact surface on the cover plate 300 may be respectively provided with a support platform 303, and the cross section of the support platform 303 may be square, circular, strip or irregular, etc.
  • the capillary gap 304 between the two is formed again, and under the action of capillary siphon, the reagent at the bottom gradually flows from the bottom to the upper part again, filling the capillary gap 304.
  • the bottom of the cover plate 300 is provided with an inclined surface 305, and the inclined surface 305 is set at an angle with the lower surface of the cover plate 300.
  • the angle between the inclined surface 305 and the lower surface of the cover plate 300 is 1° to 25°, more preferably 5° to 10°, and more preferably, the angle is 8°.
  • the inclined surface 305 at the bottom is fitted with the slide 600, reducing the outflow of the reagent from the slide 600 and the bottom of the cover plate 300, and avoiding the loss of the reagent.
  • the angle between the inclined surface 305 and the lower surface of the cover plate 300 can also be other angles.
  • the bottom plate 200 includes a first bottom plate 201 and a second bottom plate 211 which are plugged into each other.
  • the lower surface of the first bottom plate 201 is a limiting surface 204, and third connecting parts 203 are arranged on both sides thereof, and the third connecting parts 203 are connected to the upper part of the side plate.
  • Fourth connecting parts 212 are arranged on both sides of the second bottom plate 211, and the fourth connecting parts 212 are connected to the bottom of the side plate.
  • the upper part of the cover plate 300 is arranged below the limiting surface 204 of the adjacent bottom plate, and the cover plate 300 can contact the limiting surface 204.
  • the spacing between the two adjacent first bottom plates 201 is the same, and the limiting surface 204 is used to limit the size of the opening between the cover plate 300 and the carrier 600.
  • the bottom of the cover plate 300 sags downward due to gravity and partially abuts against the slide 600.
  • the opening between the cover plate 300 and the slide 600 cannot be further enlarged due to the restriction of the limited position surface 204.
  • the cover plate 300 is simultaneously subjected to the downward pressure of the limit surface 204 and the upward support force of the first support surface 104 and the second support surface 405. Under the combined action of these forces, the bottom of the cover plate 300 is forced to completely tilt up, so that the cover plate 300 is completely separated from the slide 600. At this time, most of the reagents are lost, which is convenient for cleaning and replacement of new reagents.
  • the upper surface of the first bottom plate 201 may be provided with a receiving slot 202 and a first isolating portion 206, and the first isolating portion 206 separates two adjacent receiving slots 202.
  • the first isolating portion 206 makes each receiving slot 202 relatively independent and isolates adjacent slides 600 to prevent cross contamination of samples or reagents on adjacent slides 600.
  • the first isolating portion 206 is provided with a guide surface 205 at the slide 600 insertion port to facilitate the slide 600 to be inserted into the receiving slot 202.
  • a fifth connection portion 403 for connecting to an external driving device is connected between the pair of pushers 400.
  • the driving device drives the fifth connection portion 403 to move, driving the pair of pushers 400 to move synchronously in the first direction A or the second direction B shown in FIG.
  • the incubation support 1 may further include a limiting platform 500 for holding the slide 600, the limiting platform 500 being arranged below the bottom plate 200 and connected between a pair of side plates to prevent the slide 600 from falling from the bottom.
  • a drainage groove 501 is provided at the position where the limiting platform 500 contacts the slide 600, so as to facilitate the drainage of waste liquid during cleaning.
  • the protrusion 301 is forced to move downward along the intersection of the first support surface 104 and the second support surface 405, gradually reducing the opening between the cover plate 300 and the slide 600, and finally reaching a state where the cover plate 300 and the slide 600 are in contact. If one of the cover plates is stuck, the protrusion 301 of the cover plate is disengaged from the cooperation with the second support surface 405 and is located at a certain position in the movable groove 404, and the other cover plates 300 that are not stuck continue to move normally. This avoids the movement of other cover plates being affected by the jamming of a certain cover plate 300. It also avoids the problem of the pusher 400 continuing to move, causing the protrusion 301 to break.
  • the pusher 400 can drive the movement of multiple cover plates 300 at the same time, and the movements of each cover plate 300 are independent of each other.
  • the cover 300 is moved by the push member 400 so that the cover 300 gradually fits or separates from the carrier 600, so that the reagents in various parts of the carrier 600 are mixed more evenly and the detection objects in the reagents combine with the detection objects in the specimen more quickly, thereby effectively improving the efficiency and accuracy of specimen processing.
  • this embodiment provides an incubation support 1a, including a support assembly 100a, a pair of pushers 400a, a base plate 200a and a cover plate 300a.
  • the connection relationship of these assemblies or components and other unmentioned parts can refer to the first embodiment.
  • the bracket assembly 400a includes a pair of side plates.
  • a pair of pushers 400a are respectively slidably connected to the pair of side plates, the bottom plate 200a and the cover plate 300a are both connected between the pair of side plates, and a limiting groove 103a is provided on the side plate.
  • Both sides of the cover plate 300a are provided with a protrusion 301a, and the protrusions 301a on both sides are respectively hinged to the pair of pushers 400a.
  • the limiting groove 103a is used to limit the distance of the reciprocating motion of the protrusion 301a.
  • the protrusion 301 is provided on the upper part of the cover plate 300.
  • the bracket assembly 100a includes a first connecting portion 105a and a second connecting portion 106a, and the side panel includes a left side panel 101a and a right side panel 102a that are symmetrically arranged.
  • the left side panel 101a, the right side panel 102a, the first connecting portion 105a and the second connecting portion 106a are described in Example 1 (left side panel 101, right side panel 102, first connecting portion 107 and second connecting portion 108), which will not be repeated here.
  • the left plate 101a and the right plate 102a are both provided with a limiting groove 103a, and the bottom plate 200a is vertically connected to the left plate 101a and the right plate 102a.
  • the limiting groove 103a has two parallel inner side walls 107a, and the inner side walls 107a are arranged to cross the upper surface of the bottom plate 200a.
  • the protrusion 301a may be a rod extending outward, and the limiting groove 103a is in a straight line shape.
  • Each straight line limiting groove 103a has the same length and a width slightly larger than the diameter of the protrusion 301a, which can limit the movement trajectory of the protrusion 301a.
  • the cover plate 300a is provided with a second isolation portion 302a, a support platform 303a and an inclined surface 305a. Please refer to the description of the second isolation portion 302a, the support platform 303a and the inclined surface 305a in the first embodiment (the second isolation portion 302, the support platform 303 and the inclined surface 305), which will not be repeated here.
  • the pusher 400a may be a slide bar or a slide plate, and the pair of pushers 400a includes a left pusher 401a and a right pusher 402a symmetrically arranged, the left pusher 401a is slidably connected to the left side plate 101a, and the right pusher 402a is slidably connected to the right side plate 102a.
  • Each pusher 400a is connected to the protrusion 301a on one side of the cover plate 300a.
  • a fourth connection portion 403a is provided between the ends of the left pusher 401a and the right pusher 402a, and the fourth connection portion 403a may be a connection rod or a connection plate.
  • the fourth connection portion 403a is connected to an external driving device, and the fourth connection portion 403a, driven by the driving device, can drive the left pusher 401a and the right pusher 402a to move synchronously in the first direction Aa or the second direction Ba shown in FIG. 14 .
  • the left pusher 401a and the right pusher 402a are both provided with a fifth connection portion 404a, which can be a through hole, and the protrusion 301a is hinged to the fifth connection portion 404a.
  • the fifth connection portions 404a on the left pusher 401a and the right pusher 402a are symmetrically arranged so that each cover plate 300a is parallel to each other. Driven by the pusher 400a, the multiple cover plates 300a move synchronously and in unison.
  • the pusher 400a is connected to the bracket assembly 100a in a sliding manner.
  • a slide groove 104a can be provided on the inner side of the side panel, and the pusher 400a can reciprocate in the slide groove 104a along the first direction Aa shown in FIG14 .
  • a slide groove 104a can also be provided on the outer side of the side panel.
  • the slide groove 104a extends from one end of the side panel to the other end of the side panel.
  • the slide groove 104a is adapted to the pusher 400a.
  • the left pusher 401a and the right pusher 402a can slide synchronously in the slide groove 104a toward the first direction Aa or the second direction Ba shown in FIG14 .
  • the slide groove 104a is not provided on the side panel.
  • the pusher 400a can also be provided on the top of the side panel, and the pusher 400a can reciprocate in the top of the side panel.
  • each base plate 200a can load one or more slides 500a to facilitate staining or detection of multiple slides 500a at the same time, effectively improving the processing efficiency.
  • a plurality of first isolation portions 203a and a plurality of receiving grooves 201a are formed on the bottom plate 200a.
  • first isolation portions 203a and the plurality of receiving grooves 201a please refer to the description of the first embodiment (the first isolation portions 206 and the receiving grooves 202), which will not be repeated here.
  • the bottom plate 200a can be integrally formed, and the third connecting parts 202a are respectively provided on both sides of the top and bottom ends of the bottom plate 200a.
  • the third connecting part 202a at the top of the bottom plate 200a is respectively connected to the top of the left plate 101a and the right plate 102a, and the third connecting part 202a at the bottom end of the bottom plate 200a is respectively connected to the bottom of the left plate 101a and the right plate 102a, and the two adjacent bottom plates 200a are parallel to each other.
  • the limiting platform 204a is arranged at the bottom of the bottom plate 200a, and is used to hold the slide 500a inserted into the bottom plate 200a, and can prevent the slide 500a from falling from the bottom of the bottom plate 200a.
  • a drainage groove 205a is provided at the bottom of the bottom plate 200a, and the drainage groove 205a is hollow. When cleaning the cover plate 300a or the slide 500a, the sewage can flow out from the drainage groove 205a at the bottom of the bottom plate 200a.
  • the bottom plate 200a may also be detachable, and includes a first bottom plate 206a and a second bottom plate 207a.
  • the limit platform 204a is disposed at the bottom of the bracket assembly 100a, and the two sides of the limit platform 204a are respectively connected to the bottom of the left plate 101a and the right plate 102a.
  • the first bottom plate 206a, the second bottom plate 207a and the limit platform 204a are described in Example 1 (the first bottom plate 201, the second bottom plate 211 and the limit platform 500), which will not be repeated here.
  • the reagent When the reagent is added to the inclined bottom plate 200a, the reagent can flow to the abutment between the cover plate 300a and the slide 500a. In the process of the cover plate 300a and the slide 500a being relatively attached or separated, the cover plate 300a and the slide 500a can stir the reagent together, so that the reagent and the specimen react more quickly, so that the staining or detection effect is better.
  • the inclined bottom plate 200a is also conducive to the water flowing out obliquely downward under the action of gravity when washing the slide 500a and the cover plate 300a with clean water.
  • the cover plate 300a includes a straight plate or a curved plate, the lower surface of the straight plate is a plane, and the lower surface of the curved plate is a curved surface. Before the straight plate or the curved plate is completely separated from the carrier 500a, a portion of the straight plate or the curved plate is always in contact with the carrier 500a.
  • the raised portion 301a on the upper portion of the straight cover plate 300a is supported by the fifth connecting portion 404a and the limiting groove 103a, while the lower portion of the straight cover plate 300a is not connected to other components.
  • the lower portion of the straight cover plate 300a sags due to gravity, and part of it rests on the slide 500a.
  • the raised portion 301a on the upper portion of the arc-shaped plate cover 300a is supported by the fifth connecting portion 404a and the limiting groove 103a, while the lower portion of the arc-shaped plate cover 300a is not connected to other components.
  • the lower portion of the arc-shaped plate cover 300a sags due to gravity, and due to the arc-shaped arc, the middle or lower portion of the arc rests on the slide 500a.
  • the push member 400a slides in the second direction Ba driven by an external force, forcing the protrusion 301a to move along the second end of the limiting groove 103a toward the first end to reduce the opening between the cover plate 300a and the carrier 500a, and finally reaching a state where the cover plate 300a is in contact with the carrier 500a.
  • the pusher 400a slides in the first direction Aa driven by an external force: the protrusion 301a is forced to move along the first end of the limiting groove 103a toward the second end, gradually increasing the opening between the cover plate 300a and the slide 500a. If it is necessary to wash or release the reagent, the pusher 400a continues to slide in the first direction Aa, and the top of the cover plate 300a is subjected to downward pressure from the lower surface of the adjacent bottom plate 200a, and the bottom of the cover plate 300a is tilted, so that the top and bottom of the cover plate 300a are separated from the slide 500a.
  • the pushing member 400a slides in the second direction Ba driven by an external force, forcing the protrusion 301a to move along the second end of the limiting groove 103a toward the first end, and the upper part of the cover plate 300a gradually fits with the carrier 500a, while the lower part of the cover plate 300a will make an opposite separation movement in a seesaw manner, and finally reach a state where the upper part of the cover plate 300a fits with the carrier 500a.
  • the pusher 400a slides in the first direction Aa driven by an external force: the protrusion 301a is forced to move along the first end of the limiting groove 103a toward the second end, and the upper part of the cover plate 300a gradually separates from the slide 500a, while the lower part of the cover plate 300a performs the opposite fitting movement in a seesaw manner, and the lower part of the cover plate 300a gradually fits with the slide 500a.
  • the pusher 400a continues to slide in the first direction Aa, and the top of the cover plate 300a is subjected to the downward pressure of the lower surface of the adjacent bottom plate 200a, and the bottom of the cover plate 300a is tilted, so that the top of the cover plate 300a and the bottom of the cover plate 300a are separated from the slide 500a.
  • the capillary gap 304a formed will cover the slide 500a more comprehensively, and the reagent will be filled in the capillary gap 304a by capillary suction, which is conducive to staining and detecting a larger range of specimens on the slide 500a.
  • an arc-shaped cover plate 300a when the upper part of the cover plate 300a is separated from or attached to the slide 500a, the lower part of the arc-shaped plate will be attached to or separated from the slide 500a in a seesaw manner. Only a part of the area between the cover plate 300a and the slide 500a is always attached, so only a part of the area forms a capillary gap 304a, and there is a capillary gap 304a filled with siphon reagent.
  • the use of an arc-shaped plate is more conducive to promoting a large-scale reciprocating motion of the reagent on the slide 500a, further enhancing the effect of dynamic incubation, shortening the incubation time, and increasing the detection sensitivity.
  • the range of the arc-shaped plate and the slide 500a can be controlled, thereby reducing the area covered by the reagent on the slide 500a and reducing the amount of reagent used, especially when performing fluorescence in situ hybridization or using other expensive reagents, which can save costs.
  • this embodiment provides an incubation support 1b, which may include a support assembly 100b, a bottom plate 200b, a cover plate 300b, and a pusher 400b, and the assembly relationship between the three and the slide is as described in Example 1.
  • the cover plate 300b can move relative to the bottom plate 200b, and the cover plate 300b slides on the slide 600b to mix the reagent between the cover plate 300b and the slide 600b.
  • the bracket assembly 100b includes a side panel, a first connecting portion 105b and a second connecting portion 106b.
  • the side panel includes a left side panel 101b and a right side panel 102b that are symmetrically arranged.
  • the left side panel 101b, the right side panel 102b, the first connecting portion 105b and the second connecting portion 106b please refer to the description in Example 1 (left side panel 101, right side panel 102, first connecting portion 107 and second connecting portion 108), which will not be repeated here.
  • the side plate is provided with a first limiting groove 103b, and the first limiting groove 103b has two parallel inner side walls 104b.
  • the cover plate 300b is provided with protrusions 301b on both sides, and the protrusions 301b slide in the first limiting groove 103b.
  • the protrusions 301b on both sides are respectively hinged to a pair of pushers 400b, and the cover plate 300b is arranged in close contact with the carrier 600b.
  • the bottom plate 200b may be integrally formed.
  • the left side plate 101b and the right side plate 102b are both provided with a first limiting groove 103b, and the first limiting groove 103b can be set as a straight groove.
  • the first limiting groove 103b has an equal length and a width slightly larger than the diameter of the protrusion 301b, which can limit the movement trajectory of the protrusion 301b.
  • the first limiting groove 103b has two parallel inner side walls 104b, and the inner side walls 104b are parallel to the upper surface of the bottom plate 200b.
  • the width of the first limiting groove 103b refers to the distance between the two parallel inner side walls 104b.
  • the first end of each first limiting groove 103b is closer to the top of the bottom plate 200b than the second end.
  • the distance between the inner side wall 104b located at the bottom of the first limiting groove 103b and the upper surface of the bottom plate 200b is slightly greater than the thickness of the carrier 500b, so that the carrier 500b can be located between the upper surface of the bottom plate 200b and the lower surface of the cover plate 300b.
  • the raised portion 301b may be a rod extending outward, and the cover plate 300b is a straight plate.
  • the cover plate 300b may refer to the description of (cover plate 300) in the first embodiment, and will not be described in detail here.
  • the cover plate 300b is provided with a second isolation portion, a support platform 303b, a capillary gap 304b and an inclined surface.
  • the second isolation portion, the support platform and the inclined surface may refer to the description of (second isolation portion 302, support platform 303, capillary gap 304 and inclined surface 305) in the first embodiment, and will not be described in detail here.
  • the pusher 400b may be a slide bar or a slide plate, and the pair of pushers 400b includes a left pusher 401b and a right pusher 402b that are symmetrically arranged.
  • the left pusher 401b is slidably connected to the left side plate 101b
  • the right pusher 402b is slidably connected to the right side plate 102b.
  • the pusher 400b is slidably connected to the bracket assembly 100b by providing a slide groove 105b on the outer side or the inner side of the side plate, and the pusher 400b can reciprocate in the slide groove 105b.
  • a second limiting groove 403b is provided on the push member 400b, and the second limiting groove 403b is connected to the protrusion 301b of the cover plate 300b, and a fourth connecting portion 404b is provided between the pair of push members 400b.
  • the fourth connecting portion 404b can be a connecting rod or a connecting plate, which is connected to the same end of the left push member 401b and the right push member 402b by screws.
  • the fourth connecting portion 404b can be connected to an external driving device, and the fourth connecting portion 404b can drive the left push member 401b and the right push member 402b to move synchronously in the first direction Ab or the second direction Bb shown in FIG26 under the drive of the driving device.
  • the second limiting groove 403b can be a straight-line limiting groove, the width of which is slightly larger than the diameter of the protrusion 301b, and the protrusion 301b can move in the second limiting groove 403b.
  • the second limiting groove 403b can also be configured as a circular hole, the diameter of which is slightly larger than the diameter of the protrusion 301b, and the protrusion 301b can rotate in the circular hole.
  • the length direction of the first limiting groove 103b is arranged to intersect with the length direction of the second limiting groove 403b, and the protrusion 301b is located in the corresponding first limiting groove 103b and the second limiting groove 403b at the same time.
  • the first end of the first limiting groove 103b is closer to the top of the bottom plate 200b than the second end, and the second end is closer to the bottom of the bottom plate 200b than the first end.
  • the second limiting groove 403b is vertically or obliquely arranged, and the first end of the second limiting groove 403b is closer to the top of the bracket assembly 100b than the second end, and the second end is closer to the bottom of the bracket assembly 100b than the first end.
  • the second limiting groove 403b can also be arranged horizontally, in which case the first end of the second limiting groove 403b is closer to the first connecting portion 106b than the second end, and the second end is closer to the second connecting portion 107b than the first end.
  • the inner side wall 104b of the second limiting groove 403b pushes the protrusion 301b, so that the protrusion 301b moves along the first limiting groove 103b from the first end to the second end.
  • the cover plate 300b slides downward on the carrier 500b until the protrusion 301b moves to the second end of the first limiting groove 103b and/or the second end of the second limiting groove 403b and stops sliding.
  • the inner side wall 104b of the second limiting groove 403b pushes the protrusion 301b, so that the protrusion 301b moves along the first limiting groove 103b from the second end to the first end.
  • the cover plate 300b slides upward on the carrier 500b until the protrusion 301b moves to the first end of the first limiting groove 103b and/or the first end of the second limiting groove 403b and stops sliding.
  • the cover plate 300b is moved by the pusher 400b, so that the cover plate 300b attached to the slide 600b can slide back and forth along the length direction of the slide 600b.
  • the specimen and reagent between the cover plate 300b and the slide 600b are stirred and mixed, so that the specimen and reagent react quickly and fully, avoiding the occurrence of uneven reagent concentration and bubble accumulation and long-term static waiting, thereby improving the efficiency and accuracy of specimen processing on the slide.
  • the cover plate 300b always slides in contact with the upper surface of the slide 500b.
  • this embodiment provides an incubation support 1c, which may include a support assembly 100c, a base plate 200c and a cover plate 300c, wherein the base plate 200c is used to load a carrier sheet 400c, and the carrier sheet 400c is located between the base plate 200c and the cover plate 300c.
  • the support assembly 100c includes an upper support 101c and a lower support 104c, both of which can adopt a plate-like structure.
  • the two sides of the upper portion of the base plate 200c are connected to the upper support 101c, and the two sides of the lower portion of the base plate 200c are connected to the lower support 104c.
  • the cover plate 300c is correspondingly arranged on one side of the base plate 200c, and the two sides of the upper portion of the cover plate 300c are connected to the upper support 101c, and the lower portion of the cover plate 300c can be abutted against the upper surface of the carrier 400c.
  • the upper support 101c can move relative to the lower support 104c under the drive of an external force, so that the upper support 101c and the lower support 104c are gradually relatively separated or brought closer to each other, so as to increase or decrease the size of the opening between the cover plate 300c and the carrier 400c.
  • the upper support 101c moves in an arc trajectory and gradually separates and moves closer to the lower support 104c, so that the cover 300c connected to the upper support 101c moves with a larger amplitude, which is beneficial to the flow of reagents between the cover 300c and the carrier 400c and the elimination of bubbles in the reagents.
  • the upper bracket 101c includes a first upper bracket 102c and a second upper bracket 103c, and the first upper bracket 102c and the second upper bracket 103c are symmetrically arranged.
  • the first upper bracket 102c and the second upper bracket 103c are parallel to each other.
  • the first upper bracket 102c and the second upper bracket 103c are both provided with a plurality of first connecting parts 108c and second connecting parts 109c.
  • the lower support 104c may include a first lower support 105c and a second lower support 106c, and the first lower support 105c and the second lower support 106c are arranged symmetrically.
  • the first lower support 105c and the second lower support 106c are arranged parallel to each other.
  • the first lower support 105c and the second lower support 106c are both provided with a plurality of first connecting portions 108c. The distance between two adjacent first connecting portions 108c is equal, and the distance between two adjacent second connecting portions 109c is equal.
  • the third connection part 202c is provided at the top and bottom of both sides of the bottom plate 200c.
  • the third connection part 202c can be a protruding shaft, and the first connection part 108c can be a round hole.
  • the third connection part 202c can rotate in the first connection part 108c.
  • the bottom plate 200c is rotatably connected between the first upper bracket 102c and the second upper bracket 103c, and is rotatably connected between the first lower bracket 105c and the second lower bracket 106c.
  • the cover plate 300c is provided with raised portions 301c on both sides, and the raised portions 301c are located at the upper portion of the cover plate 300c.
  • the raised portions 301c are rotatably connected to the second connecting portion 109c, and the cover plate 300c is connected between the first upper bracket 102c and the second upper bracket 103c through the raised portions 301c. Since the upper portion of the cover plate 300c has a supporting force, and the lower portion of the cover plate 300c is not connected to the lower bracket 104c, the lower portion of the cover plate 300c droops downward under the action of gravity, and can be attached to the upper surface of the carrier 400c or suspended in the air.
  • a support platform 303c, an inclined surface 305c, a capillary gap 304c and a second isolation portion are also provided on the cover plate 300c.
  • the support platform 303c, the inclined surface 305c, the capillary gap 304c and the second isolation portion please refer to the description of Example 1 (support platform 303, inclined surface 305, capillary gap 304 and second isolation portion 302), which will not be repeated here.
  • the support assembly 100c may further include a plurality of connecting members 107c, which may be connecting rods.
  • the connecting members 107c may be disposed at both ends of the first upper support 102c and the second upper support 103c.
  • the connecting members 107c may be disposed at both ends and a middle position of the first lower support 105c and the second lower support 106c.
  • the bottom plate 200c is integrally formed, and the bottom plate 200c is provided with a receiving groove 201c, a first isolation portion 203c, a limit platform 204c and a drainage groove 205c.
  • the receiving groove 201c, the first isolation portion 203c, the limit platform 204c and the drainage groove 205c are described in Example 2 (the receiving groove 201a, the first isolation portion 203a, the limit platform 204a and the drainage groove 205a in the integrally formed bottom plate 200a), which will not be repeated here.
  • the upper support 101c moves relative to the lower support 104c in the first direction Ac shown in FIG. 30 under the drive of external force, the upper support 101c and the lower support 104c gradually separate, so that the opening between the cover plate 300c and the slide 400c gradually increases.
  • the upper support 101c continues to move until the angle between the cover plate 300c and the lower support 104c is close to, greater than or equal to 90°, the cover plate 300c and the slide 400c are completely separated, the intersection of the bottom of the cover plate 300c and the lower part of the slide 400c disappears, and the reagent or cleaning liquid can flow away from between the cover plate 300c and the slide 400c.
  • the upper support 101c is connected to an external driving device, and driven by the external driving device, the upper support 101c can move relative to the lower support 104c along the first direction Ac or the second direction Bc.
  • the upper part of the cover plate 300c and the upper part of the slide 400c are driven to separate, the capillary gap 304c is opened, and the reagent in the capillary gap 304c flows to the angle between the bottom of the cover plate 300c and the slide 400c due to the action of gravity.
  • the upper part of the cover plate 300c and the upper part of the slide 400c will be re-attached to re-form the capillary gap 304c, and the reagents gathered between the bottom of the cover plate 300c and the lower part of the slide 400c will diffuse into the formed capillary gap 304c due to the effect of capillary siphon.
  • This cycle is repeated, so that the reagents are in a flowing state between the cover plate 300c and the slide 400c, which has the dynamic incubation effect of stirring evenly, eliminating bubbles, and the reagents fully covering the specimens.
  • the combination of the detection object in the reagent and the object to be detected in the specimen is faster, and the non-specific binding is less, which effectively improves the quality and efficiency of the specimen processing of the slide 400c.
  • an incubator 1d which includes a housing mechanism 100d, a driving mechanism 200d and an incubation mechanism 300d.
  • the housing mechanism 100d is provided with an installation cavity, and the driving mechanism 200d and the incubation mechanism 300d are arranged in the installation cavity.
  • the driving mechanism 200d can drive the cover plate 343d in the incubation mechanism 300d to move relative to the slide 345d to mix the reagent between the cover plate 343d and the slide 345d, realize dynamic incubation, and improve the efficiency and quality of specimen processing on the slide 345d.
  • the incubation mechanism 300d includes a support assembly 310d and an incubation bracket 340d.
  • the support assembly 310d is connected to the inner wall of the installation cavity, and the support assembly 310d is movably connected to the inner wall of the installation cavity in a push-pull manner.
  • the support assembly 310d includes an incubation pot 311d provided with an incubation groove 312d, and the incubation bracket 340d is removably arranged in the incubation groove 312d, and the support assembly 310d can drive the incubation bracket 340d to move forward and backward.
  • the incubation bracket 340d includes a cover plate 343d and a slide 345d. Please refer to the description of the incubation bracket 340d in the above-mentioned embodiments one to four, which will not be repeated here.
  • the support component 310d has the function of being opened and closed relative to the shell mechanism 100d, that is, the support component 310d can be installed in the shell mechanism 100d like a drawer, and the incubation bracket 340d and the incubation trough 312d are detachably connected, which facilitates the disassembly and assembly of the incubation bracket 340d and the incubation pot 311d.
  • the driving mechanism 200d includes a driving member 210d and a driven member 220d, wherein the driving member 210d is drivingly connected to the driven member 220d.
  • the driven member 220d is drivingly connected to the cover plate 343d and drives the cover plate 343d to move relative to the carrier 345d.
  • the housing mechanism 100d may include a front plate 110d, a rear plate 120d, a first side plate 130d, a second side plate 140d, an upper cover 150d, and a base plate 160d.
  • a mounting cavity is formed by connecting the front plate 110d, the rear plate 120d, the first side plate 130d, the second side plate 140d, the upper cover 150d, and the base plate 160d, and a fan 161d may be provided in the mounting cavity.
  • the upper cover 150d is hinged to the first side plate 130d, and the upper cover 150d may be opened.
  • the front plate 110d is provided with an opening for the incubation mechanism 300d to enter and exit.
  • connection between the upper cover 150d and the first side plate 130d is not limited to a reversible connection, but may also be a fixed connection.
  • one edge of the upper cover 150d is fixedly connected to the first side plate 130d, and the upper cover 150d may have the same structure as a shutter, with the functions of opening and closing, so that the upper cover 150d can be opened and closed.
  • heat dissipation holes can be provided on the base plate 160d and the back plate 120d to cool down and protect the electronic components in the housing structure.
  • a plurality of drip holes 151d are provided on the upper cover 150d, and the drip holes 151d are through holes, and the upper cover 150d is located above the incubation pot 311d.
  • the incubation pot 311d can be loaded with an incubation bracket 340d, and the incubation bracket 340d includes a cover plate 343d and a carrier 345d.
  • the plurality of drip holes 151d correspond to the plurality of carriers 345d below one by one, and the drip holes 151d are aligned with the upper part of the carrier 345d directly below, and the reagent drips from the drip holes 151d between the carrier 345d and the cover plate 343d.
  • the upper cover 150d may not be provided with a drip hole 151d.
  • the upper cover 150d can be opened and the reagent can be dripped between the carrier 345d and the cover plate 343d.
  • the support assembly 310d may include a support frame 315d, a pair of slide rails and an incubation pot 311d, the pair of slide rails are respectively connected to the two sides of the support frame 315d, the incubation pot 311d is connected to the support frame 315d, and an incubation trough 312d is provided in the incubation pot 311d, and the incubation trough 312d can load the incubation bracket 340d.
  • the support frame 315d may include a first support plate 316d, a second support plate 317d, a third support plate 318d and a cross beam 319d, the third support plate 318d is arranged in front of the cross beam 319d, and the two ends of the third support plate 318d are respectively connected to the front ends of the first support plate 316d and the second support plate 317d, the two ends of the cross beam 319d are respectively connected to the rear ends of the first support plate 316d and the second support plate 317d, and the cross beam 319d is used to support the bottom of the incubation pot 311d.
  • connection mode of the incubation pot 311d and the support frame 315d can be a fixed connection or a supporting connection.
  • nuts and bolts can be used to fix the four sides of the incubation pot 311d with the first support plate 316d, the second support plate 317d, the third support plate 318d and the crossbeam 319d.
  • a step groove is provided on the upper edge of the first support plate 316d, the second support plate 317d and the third support plate 318d, so that the upper edge of the pot body of the incubation pot 311d can be supported in the step groove, and the bottom of the incubation pot 311d is supported on the crossbeam 319d, which can be more convenient when the incubation pot 311d needs to be replaced.
  • a pair of slide rails may include a first slide rail 321d and a second slide rail 322d, the first support plate 316d and the first side plate 130d are connected on both sides of the first slide rail 321d, the second support plate 317d and the second side plate 140d are connected on both sides of the second slide rail 322d, and by pulling the third support plate 318d forward and backward, the support frame 315d can move forward and backward under the guidance of the first slide rail 321d and the second slide rail 322d.
  • the support frame 315d is slidably connected to the side wall of the installation cavity through a pair of slide rails, and the incubation pot 311d is connected to the support frame 315d, so that the support frame 315d can drive the incubation pot 311d to slide out of the installation cavity, and then drive the incubation bracket 340d to move back and forth, so as to facilitate the removal of the incubation bracket 340d from the incubation tank 312d, and facilitate the loading of tissue samples and the cleaning of the incubation bracket 340d.
  • the support assembly 310d and the inner wall of the installation cavity can also be connected in other ways.
  • the support assembly 310d and the inner wall of the installation cavity can be fixedly connected.
  • the support assembly 310d can include a support frame 315d and an incubation pot 311d, the support frame 315d is fixedly connected to the inner walls on both sides of the installation cavity, the incubation pot 311d is connected to the support frame 315d, the upper cover 150d of the housing mechanism 100d is flipably connected to the first side plate 130d, and the upper cover 150d can open and close relative to the second side plate 140d. When the upper cover 150d is opened, it is convenient to take the incubation bracket 340d out of the incubation pot 311d.
  • the supporting assembly 310d may further include a front panel 320d, on which a display screen may be provided, and the display screen may be a touch screen, which may not only display the working status information of the incubator 1d, but also perform control operations on the display screen.
  • Support assembly 310d can also include heating element 323d and heat insulation element 324d, heating element 323d is connected to the bottom of incubation pot 311d, and is fixedly connected to support frame 315d. Heating element 323d is used to heat the pot body of incubation pot 311d, and then can heat the water in incubation tank 312d. Heat insulation element 324d is arranged below heating element 323d and is connected to the bottom of support frame 315d, and isolation plate mainly plays the role of heat insulation protection, avoids heating element 323d in the heating process, the temperature is too high and damages other electronic components in the installation cavity. It should be noted that heating element 323d can be a heating device such as a heating plate, a heating film, and heat insulation element 324d can be a heat insulation board. Heating can also be external heating and transported into the incubation pot through a pipeline.
  • the support assembly 310d includes a plurality of incubation pots 311d arranged side by side, each of the incubation pots 311d is provided with an incubation groove 312d, and each of the incubation grooves 312d can be loaded with at least one incubation bracket 340d.
  • a limiting column 313d is provided at the bottom of the incubation groove 312d, and a limiting groove 346d is provided at the bottom of the incubation bracket 340d.
  • the limiting column 313d can cooperate with the limiting groove 346d to limit the shaking of the incubation bracket 340d.
  • the incubation support 340d includes a support assembly 341d, a push-pull assembly 342d, a cover plate 343d, a bottom plate 344d and a slide 345d.
  • the cover plate 343d can move relative to the slide 345d by pushing the push-pull assembly 342d forward and backward, so that the reagent between the cover plate 343d and the slide 345d can be stirred, so that the reagent accelerates the reaction with the tissue sample on the slide 345d.
  • the support assembly 341d may include a pair of side panels, and the bottom of the two side panels is provided with a limiting groove 346d, and each side panel may be provided with two limiting grooves 346d, and the limiting groove 346d is semi-cylindrical.
  • a plurality of limiting posts 313d are provided at the bottom of the incubation tank 312d, and the limiting posts 313d can correspond to the limiting grooves 346d one by one, and the limiting grooves 346d are adapted to the limiting posts 313d, and the limiting posts 313d can be inserted into the limiting grooves 346d, and the shaking of the incubation support 340d forward and backward and left and right is limited by two or more limiting posts 313d.
  • the distance between two adjacent limiting posts 313d, or the distance between the limiting posts 313d and the side of the incubation tank 312d is slightly greater than the width of the incubation support 340d.
  • the distance between two adjacent limiting posts 313d is equal to the distance between two limiting grooves 346d on the same side of the incubation support 340d.
  • the push-pull assembly 342d may include a pair of pushers and a connecting portion.
  • the pushers and the connecting portion may refer to the descriptions in Embodiments 1 to 3 (e.g., the pusher 400 and the fifth connecting portion 403, etc.), which will not be described in detail here.
  • the push-pull assembly 342d may include an upper bracket and a connecting portion.
  • the upper bracket and the connecting portion may refer to the descriptions in Embodiment 4 (the upper bracket 101c and the connecting member 107c), which will not be described in detail here.
  • a water inlet hole 335d is provided on the rear side wall of the incubation tank 312d, and a water inlet joint 336d is provided on the rear side of the incubation pot 311d, the water inlet hole 335d is connected to the water inlet joint 336d, and the water inlet joint 336d is connected to the water inlet pump.
  • a water outlet hole 337d is provided on the bottom of the incubation tank 312d, and a water outlet joint 338d is provided on the bottom of the incubation pot 311d, the water outlet hole 337d is connected to the water outlet joint 338d, and the water outlet joint 338d is connected to the water outlet pump.
  • the driving mechanism 200d is drivingly connected to the incubation support 340d, and drives the driven member 220d to move through the driving member 210d, so as to drive the cover plate 343d to move relative to the carrier 345d.
  • the support assembly 310d further includes a connecting assembly 330d, which includes a first push plate 331d, a second push plate 332d, a connecting rod 333d and an elastic member 334d.
  • the second push plate 332d can be a plate-shaped object made of a magnet
  • the connecting portion at the upper end of the push-pull assembly 342d can also be made of a material that can be attracted by a magnet, such as iron, nickel, cobalt, etc.
  • the elastic member 334d can be a spring, etc.
  • the first push plate 331d and the second push plate 332d are connected to the two ends of the connecting rod 333d, respectively.
  • a connecting rod hole 314d is provided at the rear side of the incubation pot 311d and at the position corresponding to the second push plate 332d.
  • the connecting rod hole 314d is a circular through hole.
  • the connecting rod 333d can pass through the connecting rod hole 314d and move forward and backward in the connecting rod hole 314d.
  • the first push plate 331d and the second push plate 332d are connected to the two ends of the connecting rod 333d, respectively.
  • the second push plate 332d is located in the incubation tank 312d.
  • the elastic member 334d is sleeved on the connecting rod 333d and is located between the incubation pot 311d and the first push plate 331d.
  • the first push plate 331d and the second push plate 332d are respectively connected to the two ends of the connecting rod 333d, so that the first push plate 331d and the second push plate 332d can be synchronously moved, and have the function of restoring the original state.
  • the follower 220d moves forward and presses the first push plate 331d forward
  • the second push plate 332d can be driven to move forward and the elastic member 334d is compressed.
  • the elastic member 334d has a tendency to restore the original state.
  • the elastic member 334d applies a backward thrust to the first push plate 331d, so that the elastic member 334d pushes the first push plate 331d to move backward, and drives the second push plate 332d to move backward to the position of the original state.
  • the follower 220d is in contact with the first push plate 331d, and the second push plate 332d is connected to the cover plate 343d.
  • the second push plate 332d can use magnetic force to attract the connection part at the end of the push-pull assembly 342d together, and the follower 220d drives the first push plate 331d to move forward and backward to drive the second push plate 332d to move forward and backward.
  • the second push plate 332d drives the connection part to move forward and backward, and the connection part drives the pusher to move forward and backward, and the pusher drives the cover plate 343d to move relative to the carrier 345d.
  • the first push plate 331d and the follower 220d are detachably connected. When the support assembly 310d is pulled out of the installation cavity, the drive assembly will not move forward with the connection assembly 330d, so that the wires on the drive assembly can be prevented from being pulled out and damaged.
  • the driving member 210 d may be a screw motor, and the driven member 220 d may be a transmission rod or a transmission plate.
  • the output end of the driving member 210 d is hinged to the lower end of the driven member 220 d, and the output end of the driving member 210 d can move forward and backward.
  • the housing mechanism 100 d may further include a mounting plate 170 d, and the middle portion of the driven member 220 d is hinged to the mounting plate 170 d, so that the upper and lower ends of the driven member 220 d can swing with the position of the hinge with the mounting plate 170 d as the axis, and when the output end of the driving member 210 d moves forward, the upper end of the driven member 220 d swings backward, and when the output end of the driving member 210 d moves backward, the upper end of the driven member 220 d swings forward. In this way, by the front and back swinging of the upper end of the driven member 220 d, the first push plate 331 d moves forward and backward, thereby driving the cover plate 343 d to move relative to the carrier 345 d.
  • the driving member 210d is fixedly connected to the base plate 160d and is located below the incubation mechanism 300d, and the driving member 210d is located within the range of the top projection of the incubation mechanism 300d.
  • the connecting assembly 330d, the driven member 220d and the driving member 210d are arranged in a U shape, which can reduce the front-to-back distance of the housing mechanism 100d and its floor space, and avoid the connecting assembly 330d, the driven member 220d and the driving member 210d being arranged in a straight line, resulting in a large front-to-back distance of the housing mechanism 100d and occupying space.
  • the driving member 210d is located within the range of the top projection of the incubation mechanism 300d, but the present disclosure is not limited to this, and the driving member 210d can also be arranged at the rear side of the incubation mechanism 300d.
  • the driving member 210d can be a stepper motor, and the driven member 220d can be an eccentric wheel.
  • the housing mechanism 100d can also include a mounting plate 170d, the driving member 210d is fixedly connected to the mounting plate 170d in a vertical manner, the output end of the driving member 210d is upward and connected to the bottom end of the eccentric wheel, and the eccentric wheel rotates when the output end of the driving member 210d rotates.
  • the periphery of the eccentric wheel abuts against the first push plate 331d, and the rotation of the eccentric wheel drives the first push plate 331d to move forward and backward, thereby causing the cover plate 343d to move relative to the carrier 345d.
  • the driving member 210d and the driven member 220d are located below the connecting assembly 330d, and are not completely within the range of the top projection of the connecting assembly 330d.
  • the driving member 210d adopts a stepper motor, which can save more costs compared to a screw motor.
  • the driving member 210d and the driven member 220d are located below the connecting assembly 330d, the present disclosure is not limited thereto.
  • the driving member 210d and the driven member 220d may also be located behind the connecting assembly 330d.
  • the driving member 210d may be a stepper motor
  • the driven member 220d may be a driven rod.
  • the stepper motor is fixedly connected to the top of the mounting plate 170d, and the output end of the stepper motor is connected to the driven rod, which abuts against the first push plate 331d.
  • the driven rod and the output shaft of the stepper motor are in a straight line and perpendicular to the first push plate 331d.
  • the output shaft of the stepper motor moves forward and backward, so that the first push plate 331d can move forward and backward.
  • the present embodiment provides a staining device, including a machine 100e, an incubator 200e and a tilting mechanism 300e arranged on the machine 100e.
  • the tilting mechanism 300e drives the incubator 200e to swing downward, so that the tilt angle Be of the cover plate 300 and the slide 600 relative to the horizontal plane Ae becomes smaller, thereby reducing the loss of reagents between the cover plate 300 and the slide 600.
  • the incubation box 200e may include a housing mechanism 210e, an incubation pot 220e and at least one group of incubation brackets, wherein the incubation pot 220e is disposed on the housing mechanism 210e, and the incubation brackets are removably disposed on the incubation pot 220e.
  • the housing mechanism 210e and the incubation pot 220e may adopt the structure in the fifth embodiment, and the incubation brackets may adopt the structures in the incubation brackets 1, 1a, 1b and 1c of the first to fourth embodiments, which will not be described in detail here. The following description is made by taking the incubation brackets adopting the structure in the first embodiment as an example.
  • the incubation box 200e, the incubation pot 220e, and the support assembly 100 remain parallel to the horizontal plane Ae.
  • the bottom plate, the cover plate, and the slide are tilted relative to the support assembly, that is, the bottom plate, the cover plate, and the slide have a certain angle with the horizontal plane.
  • the inclination angle Be of the bottom plate can be 15° to 90°, and correspondingly, the inclination angle Be of the slide 600 is 15° to 90°.
  • an incubation support with a bottom plate inclination angle Be of 35° is selected, and the inclination angle Be of the slide 600 is also 35°.
  • the incubator 200e swings downward to the incubation state, the incubator 200e forms a certain angle with the horizontal plane Ae, while the angles between the bottom plate, the slide and the cover plate and the horizontal plane Ae become smaller.
  • the inclination angle Be between the slide 600 and the horizontal plane Ae in the incubation state is smaller than the inclination angle Be between the slide 600 and the horizontal plane Ae in the initial state.
  • an incubation bracket with a bottom plate 200 inclination angle Be of 35° can be selected.
  • the inclination angle Be of the slide 600 relative to the horizontal plane Ae is 35°.
  • the inclination angle Be between the slide 600 and the horizontal plane Ae is 0° (that is, the slide 600 is parallel to the horizontal plane Ae). It should be noted that in the incubation state, the inclination angle Be between the slide 600 and the horizontal plane Ae can also be 2°, 3°, 4°, etc.
  • the incubator 200e includes a driving assembly, and the cover plate 300 can move relative to the slide 600 under the drive of the driving assembly.
  • the driving assembly may include a driving motor 241e and a connecting plate 242e.
  • the driving motor 241e may be arranged in the incubator 200e, and one end of the connecting plate 242e is connected to the output end of the driving motor 241e, and the other end is connected to the pusher 400.
  • the driving motor 241e drives the connecting plate 242e to move forward and backward to drive the pusher 400 to slide forward and backward in the slide groove 105.
  • the driving motor 241e and the connecting plate 242e are described in the fifth embodiment, and will not be repeated here.
  • the tilting mechanism 300e includes a support plate 320e and a driving member 310e. Both sides of the support plate 320e are hinged to the machine platform 100e.
  • the driving member 310e is disposed on the machine platform 100e and connected to the bottom of the support plate 320e to drive the support plate 320e to swing.
  • the incubator 200e is disposed on the support plate 320e.
  • An operating platform 110e is provided on the machine 100e.
  • a through slot 111e is provided on the operating platform 110e.
  • the through slot 111e can be square, rectangular, etc., and its shape is adapted to the support plate 320e.
  • a pair of support plates 120e extending downward are provided on the edges of both sides of the through slot 111e, and the support plate 320e is hingedly provided between the pair of support plates 120e.
  • the support plate 120e and the support plate 320e are provided with shaft holes at the hinge points, and are connected by a coupling 122e (which can be a coupling bolt, a shaft rod, etc.), and the support plate 320e swings around the coupling 122e.
  • the support plate 320e In the initial state, the support plate 320e is parallel to the horizontal plane Ae, and in the incubation state, the support plate 320e forms a certain angle with the horizontal plane Ae.
  • the support plate 120e is provided with an arc groove 121e at a position away from the hinge point, and the center of the arc groove coincides with the hinge point.
  • Guide rods 321e protruding outward are provided on both sides of the support plate 320e, and the guide rods 321e can slide in the arc groove 121e.
  • the arc track length of the arc groove 121e is about 23 cm, and thus the maximum distance of the arc track of the support plate 320e swinging downward can be 23 cm.
  • the tilt mechanism 300e includes a base 330e, which is arranged in the machine 100e.
  • the two ends of the driving member 310e are respectively hinged with the base 330e and the support plate 320e.
  • the driving member 310e is an electric push rod
  • the top end of the driving member 310e is hinged with the tip of the triangular connecting frame 340e, and the plane end of the triangular connecting frame 340e is connected to the bottom surface of the support plate 320e.
  • the bottom end of the driving member 310e is hinged with the tip of the triangular connecting frame 340e, and the plane end of the triangular connecting frame 340e is connected to the top surface of the base 330e.
  • the driving member 310e is connected to the bottom of the support plate 320e, but the present disclosure is not limited to this.
  • the driving member 310e can be a reduction stepper motor or a servo, which is arranged at the hinge point of the support plate 120e and the support plate 320e, and is drivingly connected to the connecting shaft 122e at the hinge point (not shown in the figure).
  • the reduction stepper motor or the servo drives the connecting shaft 122e to rotate to adjust the inclination of the support plate 320e and the horizontal plane Ae.
  • a mechanical arm 400e and a reagent bottle 420e are provided on the operating platform 110e, and the mechanical arm 400e can move in the front-back, left-right, and top-bottom directions.
  • a sample adding needle 410e is connected to the mechanical arm 400e, and the mechanical arm 400e drives the sample adding needle 410e to move freely above the operating platform 110e.
  • the mechanical arm 400e can move the sample adding needle 410e into the reagent bottle 420e, the reagent in the reagent bottle 420e is extracted. And the mechanical arm 400e moves above the cover plate 300 and the slide 600 to drip the reagent into the gap 304 between the cover plate 300 and the slide 600.
  • a water injection device (not shown) and a liquid discharge device (not shown) are provided in the installation cavity of the incubator 200e, and the incubator 220e is provided with a water injection port and a liquid discharge port, the water injection device is connected to the water injection port, and the liquid discharge device is connected to the liquid discharge port.
  • a water tank for storing clean water and a waste liquid tank for collecting waste liquid can also be provided below the operating platform 110e.
  • the water injection device can extract clean water in the water tank into the incubator 220e, and the liquid discharge device can extract waste liquid in the incubator 220e into the waste liquid tank.
  • a host computer is provided on one side of the machine 100e, and the host computer is electrically connected to the incubator 200e, the robotic arm 400e and the tilting mechanism 300e respectively.
  • the host computer can be a computer to control the working status of the incubator 200e, the robotic arm 400e and the tilting mechanism 300e.
  • This embodiment provides a dyeing method, which can be applied to the above-mentioned dyeing equipment.
  • the method may include:
  • Step S102 adding liquid to the gap 304 between the cover plate 300 and the slide 600.
  • the incubator 200e is in the initial state, and the incubator 200e and the support plate 320e are parallel to the horizontal plane Ae.
  • the incubator 200e and the support plate 320e are parallel to the horizontal plane Ae.
  • Step S104 the tilting mechanism 300e drives the incubator 200e to swing downward to the incubation state, and the tilting mechanism 300e stops driving. Specifically, during the downward swinging of the incubator 200e, the inclination angle Be between the incubator 200e and the horizontal plane Ae becomes larger, while the inclination angle Be between the cover plate 300 and the slide 600 and the horizontal plane Ae becomes smaller.
  • an incubation bracket with an inclination angle Be of 35° for the bottom plate 200 is selected, and the inclination angle Be between the slide 600 and the horizontal plane Ae is set to 0° in the incubation state.
  • the driving member 310e drives the incubator 200e to swing downward to the incubation state, and the inclination angle Be between the slide 600 and the horizontal plane Ae changes from 35° to 0°.
  • step S106 the cover plate 300 moves relative to the slide 600 to mix the liquid between the cover plate and the slide.
  • the driving motor 241e drives the pusher 400 to move back and forth in the slide 105 to drive the cover plate to move relative to the slide, so that the reagent and the specimen between the cover plate and the slide are fully reacted, or the residue between the cover plate and the slide is fully stirred and washed with clean water.
  • the movement of the cover plate relative to the slide varies according to the structure of the incubation support in Embodiments 1 to 4, such as the opening and closing movement and the fitting movement of the plate relative to the slide.
  • the opening and closing movement is that the bottom of the cover plate abuts against the slide, and the top of the cover plate moves closer to and away from the slide;
  • the fitting movement is that the cover plate fits the slide, and the cover plate reciprocates along the length direction of the slide.
  • step S108 the tilting mechanism 300e drives the incubator 200e to swing upward, and when the incubator 200e is swung to be parallel to the horizontal plane Ae, the tilting mechanism 300e stops driving. Specifically, the driving member 310e drives the support plate 320e to swing upward, so that the incubator 200e changes from the incubation state to the initial state.
  • the tilt angle Be between the slide and the horizontal plane Ae changes from 0° to 35°.
  • the staining method can be applied to the detection of slide specimens, including all or individual steps of the processing process of dewaxing, washing, repairing, adding primary antibody reagents, adding blocking agents, adding secondary antibody reagents, adding colorants, adding hematoxylin and reblueing.
  • the incubator 200e is driven to swing downward by the tilting mechanism 300e, so that the tilt angle Be of the cover plate and the slide relative to the horizontal plane Ae becomes smaller.
  • This can avoid the risk of the reagent between the cover plate and the slide being lost from the bottom of the gap due to the tilt angle Be between the cover plate and the slide being too large during the incubation process, thereby saving the amount of reagent, eliminating the need to add reagents multiple times, and improving the detection efficiency.
  • the present disclosure provides an incubation device, including a base 100f, an incubation support 200f and a tilting mechanism 300f.
  • the tilting mechanism 300f is disposed on the base 100f and is drivingly connected to the incubation support 200f.
  • the tilting mechanism 300f and the incubation mechanism are respectively arranged on the base 100f, and the driving mechanism 400f is connected to the tilting mechanism 300f.
  • the base 100f may include a base 110f and two symmetrically arranged support columns 120f, the support columns 120f are arranged at the left end of the base 100f, and the driving mechanism 400f is arranged at one end away from the support columns 120f, and the tail of the incubation bracket 200f faces the driving mechanism 400f.
  • the tilt mechanism 300f includes a first driving member 320f and a support plate 310f, one end of which is hinged to the two support columns 120f.
  • the two support columns 120f are respectively provided with shaft rods 121f
  • the two shaft rods 121f are symmetrically arranged
  • through holes are provided at positions corresponding to the shaft rods 121f on the support plate 310f
  • the support plate 310f and the two support columns 120f can be hinged through the shaft rods 121f and the through holes.
  • the first driving member 320f can be a telescopic motor, the output end of the telescopic motor is connected to one end of the support plate 310f (the end away from the support column 120f), and the bottom of the telescopic motor is connected to the base 110f.
  • the driving mechanism 400f is connected to one end of the support plate 310f.
  • the driving mechanism 400f may include a third driving member 410f and a connecting block 420f.
  • the third driving member 410f may be an eccentric motor.
  • the third driving member 410f is connected to the connecting block 420f.
  • the connecting block 420f is indirectly connected to the cover plate 220f. When the third driving member 410f drives the connecting block 420f to move forward and backward, the connecting block 420f can drive the cover plate 220f to move relative to the slide 230f, so as to speed up the reaction speed between the specimen and the reagent and improve the detection efficiency.
  • the working process of the incubation device can be: in the initial state, the support plate 310f remains parallel to the horizontal plane, and the support plate 310f is supported by two support columns 120f and the first driving member 320f. At this time, liquid can be added to the gap between the cover plate 220f and the carrier 230f.
  • the output end of the first driving member 320f is retracted, and the support plate 310f swings downward along the shaft 121f, and correspondingly, the incubation support 200f swings downward synchronously.
  • the first driving member 320f stops driving.
  • the inclination angle of the carrier 230f with the horizontal plane becomes smaller to reduce the risk of the reagent between the cover plate 220f and the carrier 230f being lost from the bottom of the gap.
  • the driving mechanism 400f can be started to work, so that the cover plate 220f moves relative to the slide 230f, so that the specimen and reagent between the cover plate 220f and the slide 230f are mixed.
  • the driving mechanism 400f stops working, and the output end of the first driving member 320f extends to drive the incubation support 200f to swing upward, and when the support plate 310f is parallel to the horizontal plane, the first driving member 320f stops driving, and the incubation support 200f is parallel to the horizontal plane.
  • the tilting mechanism 300f and the incubation mechanism are respectively arranged on the base 100f, and the driving mechanism 400f is connected to the tilting mechanism 300f.
  • the base 100f may include a base 110f and two symmetrically arranged support columns 120f, the support columns 120f are arranged at the left end of the base 100f, and the driving mechanism 400f is also arranged near one end of the support column 120f, and the tail of the incubation bracket 200f faces the driving mechanism 400f.
  • the tilt mechanism 300f may include a first driving member 320f and a support plate 310f, one end of which is hinged to the two support columns 120f.
  • the two support columns 120f are respectively provided with shaft rods 121f
  • the two shaft rods 121f are symmetrically arranged
  • through holes are provided at positions corresponding to the shaft rods 121f on the support plate 310f
  • the support plate 310f and the two support columns 120f can be hinged by the shaft rods 121f and the through holes.
  • the first driving member 320f may be a telescopic motor, the output end of the telescopic motor is connected to one end of the support plate 310f away from the support column 120f, and the bottom of the telescopic motor is connected to the base 110f.
  • the driving mechanism 400f is connected to one end of the support plate 310f.
  • the driving mechanism 400f may include a third driving member 410f and a connecting block 420f.
  • the third driving member 410f may be an eccentric motor.
  • the third driving member 410f is connected to the connecting block 420f.
  • the connecting block 420f is indirectly connected to the cover plate (for specific connection methods, please refer to the introduction of the incubation support 200f below).
  • the connecting block 420f can drive the cover plate to move relative to the slide to speed up the reaction speed of the specimen and the reagent and improve the detection efficiency.
  • the working process of the incubation device can be: in the initial state, the support plate 310f remains parallel to the horizontal plane, and the support plate 310f is supported by two support columns 120f and the first driving member 320f. At this time, liquid can be added to the gap between the cover plate and the slide.
  • the output end of the first driving member 320f extends out, and the support plate 310f swings upward along the shaft 121f, and correspondingly, the incubation support 200f swings upward synchronously.
  • the first driving member 320f stops driving.
  • the driving mechanism 400f can be started to work, so that the cover plate moves relative to the slide, so that the specimen and reagent between the cover plate and the slide are mixed.
  • the driving mechanism 400f stops working, and the output end of the first driving member 320f contracts to drive the incubation bracket 200f to swing downward, and when it swings to the support plate 310f parallel to the horizontal plane, the first driving member 320f stops driving, and at this time the incubation bracket 200f is parallel to the horizontal plane.
  • the tilting mechanism 300f and the incubation mechanism are respectively arranged on the base 100f, and the driving mechanism 400f is connected to the tilting mechanism 300f.
  • the base 100f may include a base 110f and two symmetrically arranged support columns 120f, the support columns 120f are arranged at the left end of the base 100f, and the driving mechanism 400f is arranged on a side away from the support columns 120f, and the tail of the incubation bracket 200f faces the driving mechanism 400f.
  • the tilt mechanism 300f may include a second drive member 321f and a support plate 310f, wherein a first connection hole and a second connection hole are provided in the middle of the support plate 310f, and a shaft rod is provided on the inner support column 120f, and the shaft rod can pass through the first connection hole and rotate in the first connection hole.
  • a through hole is provided on the outer support column 120f, and the second drive member 321f is connected to the support column 120f, and the drive shaft of the second drive member 321f passes through the through hole and is connected to the second connection hole.
  • the second drive member 321f may be a reduction stepping motor or a steering gear, and the second drive member 321f may drive the support plate 310f to rotate along the shaft rod to adjust the inclination of the support plate 310f and the horizontal plane, wherein the axis of the shaft rod is the same as the axis of the drive shaft of the second drive member 321f.
  • a support plate 130f is provided on the base 100f, and the support plate 130f is located on the side of the support plate 310f.
  • An arc groove 131f is opened on the support plate 130f.
  • the support plate 310f is provided with a guide rod 313f extending outward, and the guide rod 313f can slide in the arc groove 131f.
  • the arc groove 131f is used to limit the up and down swinging distance of the support plate 310f.
  • the driving mechanism 400f is connected to one end of the support plate 310f.
  • the driving mechanism 400f may include a third driving member 410f and a connecting block 420f.
  • the third driving member 410f may be an eccentric motor.
  • the third driving member 410f is connected to the connecting block 420f.
  • the connecting block 420f is indirectly connected to the cover plate (for specific connection methods, please refer to the introduction of the incubation support 200f below).
  • the connecting block 420f can drive the cover plate to move relative to the slide to speed up the reaction speed of the specimen and the reagent and improve the detection efficiency.
  • the working process of the incubation device can be: in the initial state, the support plate 310f remains parallel to the horizontal plane, and the support plate 310f is supported by two support columns 120f and a support plate 130f. At this time, liquid can be added to the gap between the cover plate and the slide.
  • the output end of the second driving member 321f rotates clockwise, and the support plate 310f swings downward along the shaft rod, and correspondingly, the incubation support 200f swings downward synchronously.
  • the second driving member 321f stops driving.
  • the driving mechanism 400f can be started to work, so that the cover plate moves relative to the slide, so that the specimen and reagent between the cover plate and the slide are mixed.
  • the driving mechanism 400f stops working, and the output end of the second driving member 321f rotates counterclockwise to drive the incubation support 200f to swing upward, and when the support plate 310f is parallel to the horizontal plane, the second driving member 321f stops driving.
  • the incubation bracket 200f When the incubation bracket 200f is in the initial state, the incubation bracket 200f remains parallel to the horizontal plane, and the inclination angle of the bottom plate 240f is set in the range of 15° to 90°, and correspondingly, the inclination angle of the slide 230f is in the range of 15° to 90°.
  • the incubation bracket 200f with a bottom plate 240f inclination of 35° (angle with the horizontal plane) is selected, and correspondingly, the inclination angle of the slide 230f is 35°.
  • the incubation bracket 200f with a bottom plate 240f inclination of 75° is selected, and correspondingly, the inclination angle of the slide 230f is 75°, and so on.
  • the incubation bracket 200f in the initial state, is horizontally placed on the support plate 310f of the tilting mechanism 300f (in the initial state, the support plate 310f is parallel to the horizontal plane), and the bottom plate 240f, the carrier 230f and the cover plate 220f all have a certain angle with the horizontal plane.
  • the tilting mechanism 300f drives the incubation bracket 200f to swing
  • the incubation bracket 200f can change from the initial state to the incubation state.
  • the incubation bracket 200f In the incubation state, the incubation bracket 200f has a certain angle with the horizontal plane, and at this time, the angle between the bottom plate 240f, the carrier 230f and the cover plate 220f in the incubation bracket 200f and the horizontal plane becomes smaller.
  • the inclination angle between the carrier 230f and the horizontal plane in the incubation state is smaller than the inclination angle between the carrier 230f and the horizontal plane in the initial state.
  • an incubation support 200f with a bottom plate 240f having an inclination angle of 35° can be selected.
  • the inclination angle of the slide 230f relative to the horizontal plane is 35°.
  • the incubation support 200f is driven by the tilting mechanism 300f to swing to the incubation state.
  • the inclination angle of the slide 230f with the horizontal plane is 0° (i.e., the slide 230f is parallel to the horizontal plane).
  • the inclination angle of the slide 230f with the horizontal plane in the incubation state can be 2°, 3°, 4°, etc.
  • the cover plate 220f and the slide 230f in the incubation bracket 200f are matched and tilted on the bracket assembly 210f, and the tilt mechanism 300f is connected to the driving of the incubation bracket 200f, and the tilt mechanism 300f can drive the incubation bracket 200f to swing to adjust the inclination of the cover plate 220f and the slide 230f relative to the horizontal plane.
  • the incubation support 200f may include a base plate 240f, a carrier 230f, a cover plate 220f, a support assembly 210f and a push rod 250f, the base plate 240f is used to load the carrier 230f, the carrier 230f is located between the base plate 240f and the cover plate 220f, and raised portions 221f are provided on both sides of the cover plate 220f.
  • the support assembly 210f includes a pair of side plates 211f, a bottom plate 240f is connected between the pair of side plates 211f, and the side plates 211f are slidably connected to the push rod 250f.
  • the push rod 250f is provided with a groove 251f, and the protrusion 221f is arranged in the groove 251f. Under the drive of external force, the push rod 250f can drive the cover plate 220f to move, so as to stir the reagent and tissue sample between the cover plate 220f and the slide 230f.
  • a connecting plate 253f is provided between the pair of push rods 250f.
  • the connecting plate 253f can be a magnetic metal. By pushing the connecting plate 253f, the pair of push rods 250f can be synchronously moved in the first direction Af.
  • the connecting block 420f on the driving mechanism 400f is also magnetic. The connecting plate 253f and the connecting block 420f can be attracted together by magnetic force. The connecting block 420f drives the connecting plate 253f to move forward and backward under the drive of the third driving member 410f.
  • the side plate 211f is provided with a slide groove 212f, and the push rod 250f is arranged in the slide groove 212f, and the push rod 250f can reciprocate in the slide groove 212f.
  • the side plate 211f is provided with a limit groove 213f and a guide groove 216f connected with the limit groove 213f, and the groove 251f is located below the guide groove 216f.
  • the groove 251f can be connected with the guide groove 216f at a certain position.
  • the structure in which the guide groove 216f, the limit groove 213f and the groove 251f can be connected facilitates the protrusion 221f to be taken out or put in from the limit groove 213f, the groove 251f and the guide groove 216f.
  • the cover plate 220f is easy to disassemble and assemble, and is convenient for replacement and cleaning, so as to avoid the cover plate 220f from being worn out due to long-term use, and the previous reagents and tissue samples being attached, thereby affecting the accuracy of subsequent detection.
  • the widths of the limit groove 213f, the guide groove 216f, and the groove 251f are all greater than the diameter of the cylinder, and the cylinder can move freely in the limit groove 213f, the guide groove 216f, and the groove 251f.
  • the bracket assembly 210f includes a pair of transverse plates 218f and a transverse rod 219f.
  • the pair of transverse plates 218f and the transverse rod 219f are described in Examples 1 to 3 (for example, the first connecting portion 107 and the second connecting portion 108, etc.) and will not be repeated here.
  • the incubation support 200f includes a plurality of base plates 240f and a plurality of cover plates 220f, and the base plates 240f correspond to the cover plates 220f one by one.
  • a plurality of accommodating grooves 241f and a plurality of raised limiting blocks 242f are provided on the base plate 240f, and limiting blocks 242f are provided on both sides of a accommodating groove 241f, and each accommodating groove 241f is provided with a carrier 230f.
  • the limiting block 242f is used to isolate two adjacent carriers 230f and limit the movement of the carrier 230f in a direction perpendicular to the base plate 240f.
  • the limiting block 242f can be a rectangle, a square, etc., and a slope (not shown in the figure) can be provided on the top of the limiting block 242f, which can guide the carrier 230f to be inserted into the accommodating groove 241f.
  • the plurality of cover plates 220f are respectively disposed above the plurality of bottom plates 240f, and a plurality of slides 230f can be inserted into each bottom plate 240f. There is always a portion of the cover plate 220f and the slide 230f in contact with each other, and the reagent is dripped between the slide 230f and the cover plate 220f.
  • the cover plate 220f is an arc-shaped plate 222f.
  • the push rod 250f slides in the first direction Af under the drive of an external force, forcing the protrusion 221f to move along the first end 214f of the limiting groove 213f toward the second end 215f, and the upper part of the arc-shaped plate 222f gradually separates from the carrier 230f, while the lower part of the cover plate 220f performs the opposite fitting movement in a seesaw manner, and a part of the cover plate 220f is always fitted with the carrier 230f.
  • the push rod 250f slides in the direction opposite to the first direction Af, forcing the protrusion 221f to move along the second end 215f of the limiting groove 213f toward the first end 214f, and the upper part of the cover 220f gradually fits with the carrier 230f, while the lower part of the cover 220f will make the opposite separation movement in a seesaw manner, and a part of the cover 220f is always in fit with the carrier 230f.
  • the use of the curved plate 222f is more conducive to promoting the large-scale reciprocating movement of the reagent on the slide 230f, strengthening the effect of dynamic incubation, shortening the incubation time, and increasing the detection sensitivity.
  • the range of the curved plate 222f and the slide 230f can be controlled, the area covered by the reagent on the slide 230f can be reduced, and the amount of reagent used can be reduced, especially when performing fluorescence in situ hybridization or using other expensive reagents, which can save costs.
  • the groove 251f can be similar to an inverted R shape, and an inclined surface 252f extends downward at the groove opening of the groove 251f.
  • the inclined surface 252f can be connected with the guide surface 217f of the guide groove 216f, so that the protrusion 221f can enter and exit the groove 251f and the limit groove 213f along the guide surface 217f and the inclined surface 252f.
  • the cover plate 220f is a straight plate 223f.
  • the push rod 250f slides in the first direction Af under the drive of an external force, forcing the protrusion 221f to move along the first end 214f of the limiting groove 213f to the second end 215f, thereby increasing the opening between the cover plate 220f and the carrier 230f.
  • the push rod 250f slides in the opposite direction of the first direction Af under the drive of an external force, forcing the protrusion 221f to move along the second end 215f of the limiting groove 213f to the first end 214f, thereby reducing the opening between the cover plate 220f and the carrier 230f.
  • the groove 251f can be similar to an inverted right-angled trapezoid, and an inclined surface 252f extends downward at the groove opening of the groove 251f.
  • a plurality of capillary planes 226f and convex strips 225f are provided on the cover plate 220f.
  • the convex strips 225f are provided on both sides of the capillary plane 226f, and the convex strips 225f extend from the top to the bottom of the capillary plane 226f.
  • the convex strips 225f are pressed against the slide 230f, and a gap is formed between the cover plate 220f and the slide 230f.
  • An isolation portion 224f is provided between two adjacent capillary planes 226f, and the isolation portion 224f can prevent the reagents on the two adjacent slides 230f from flowing across the boundary, avoiding cross contamination of the reagents, and improving the accuracy of the detection.
  • the incubation support 200f may also adopt the structures of the incubation supports 1, 1a, 1b and 1c of embodiments 1 to 4.

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Abstract

一种孵育支架（1）、孵育装置、孵育箱（200e）、染色设备及染色方法，属于生物检测技术领域。其中，孵育支架（1）包括支架组件（100）以及倾斜设置于支架组件（100）上的底板（200）和盖板（300），底板（200）用于装载载片（600），载片（600）位于底板（200）和盖板（300）之间；盖板（300）贴合在载片（600）上滑动，或者，盖板（300）相对载片（600）开合运动，以混匀盖板（300）与载片（600）之间的试剂，使得检测更加快速、均匀，提高载片（600）上标本处理的效率和准确性。

Description

一种孵育支架、孵育装置、孵育箱、染色设备及染色方法 技术领域
本公开实施例涉及生物检测技术领域，尤其涉及一种孵育支架、孵育装置、孵育箱、染色设备及染色方法。
 背景技术
相关技术中，进行染色或检测时多为静态孵育，就是将试剂滴加到载片标本上静置孵育一定时间，再进行清洗和滴加下一个步骤试剂，这种静置孵育方法，试剂中检测物与标本中的被检测物的结合速度慢，试剂浓度和分布不均，局部气泡聚集，影响检测效率、灵敏度和准确性。
需要说明的是，在上述背景技术部分公开的信息仅用于加强对本公开的背景的理解，因此可以包括不构成对本领域普通技术人员已知的现有技术的信息。
 发明内容
本公开的目的在于提供一种孵育支架、孵育装置、孵育箱、染色设备及染色方法，至少在一定程度上克服由于相关技术中静态孵育时，试剂与标本反应时间久而影响检测效率和准确性的问题。
本公开的其他特性和优点将通过下面的详细描述变得显然，或部分地通过本公开的实践而习得。
根据本公开的一个方面，提供一种孵育支架，能够装载载片，孵育支架包括支架组件以及倾斜设置于所述支架组件上的底板和盖板，所述底板用于装载所述载片，所述载片位于所述底板和所述盖板之间；所述盖板贴合在所述载片上滑动，或者，所述盖板相对所述载片开合运动，以混匀所述盖板与所述载片之间的试剂。
根据本公开的另一个方面，提供一种孵育装置，包括基座、倾斜机构和上述的孵育支架，倾斜机构设置于所述基座上，倾斜机构与所述动支架组件驱动连接，倾斜机构用于驱动所述支架组件摆动，以调整所述底板、所述盖板和所述载片相对于水平面的倾斜角。
根据本公开的又一个方面，提供一种孵育箱，包括壳体机构、驱动机构和孵育机构，壳体机构设有安装腔；驱动机构包括驱动件和从动件，所述驱动件与所述从动件驱动连接；孵育机构包括支撑组件和上述的孵育支架，所述支撑组件与所述安装腔的内壁连接；所述支撑组件包括设置有孵育槽的孵育锅，所述孵育支架设置于所述孵育槽；其中，所述盖板与所述传动件传动连接；所述驱动件能够驱动所述从动件运动，以带动所述盖板相对于所述载片运动。
根据本公开的再一个方面，提供一种染色设备，包括机台、如上述的孵育箱和倾斜机构，倾斜机构设置于所述机台上，与所述孵育箱驱动连接，能够驱动所述孵育箱向下摆动，以使所述支架组件、所述盖板、所述底板和所述载片相对于水平面的倾斜度变小。
根据本公开的还一个方面，提供一种染色方法，应用于如上述的染色设备，所述方法包括：向盖板与载片之间的缝隙添加液体；倾斜机构驱动孵育箱向下摆动至孵育状态，所述倾斜机构停止驱动；所述盖板相对所述载片运动，以使所述盖板与所述载片间的液体混匀；所述倾斜机构驱动所述孵育箱向上摆动，且摆动至所述孵育箱与水平面平行时，所述倾斜机构停止驱动。
应当理解的是，以上的一般描述和后文的细节描述仅是示例性和解释性的，并不能限制本公开。
 附图说明
 通过参照附图详细描述其示例实施方式，本公开的上述和其它特征及优点将变得更加明显。
图1示出实施例一中孵育支架在第一视角下的结构示意图。
图2是图1中孵育支架在第二视角下的结构示意图。
图3是图2中孵育支架在C-C方向的剖面结构示意图。
图4是实施例1中另一种孵育支架在C-C方向的剖面结构示意图。
图5是图2中孵育支架在D-D方向的剖面结构示意图。
图6是图1中盖板与载片贴合或分开过程中的结构示意图。
图7是图1中盖板与载片分开时的结构示意图。
图8是图1中盖板与载片贴合时的结构示意图。
图9是图1中左侧板和左驱动件的结构示意图。
图10是图1中盖板的结构示意图。
图11是图1中第一底板的结构示意图。
图12是图1中第二底板的结构示意图。
图13是图1中限位台的结构示意图。
图14示出实施例二中孵育支架在第一视角下的结构示意图。
图15示出实施例二中另一种孵育支架的结构示意图。
图16是图14中直板与载片贴合或分开过程中的结构示意图。
图17是图14中直板与载片贴合时的结构示意图。
图18是图14中直板与载片分开时的结构示意图。
图19是图14中弧形板上部与载片贴合或分开过程中的结构示意图。
图20是图14中弧形板上部与载片贴合时的结构示意图。
图21是图14中弧形板与载片分开时的结构示意图。
图22是图14中盖板的结构示意图。
图23是图14中底板的结构一种示意图。
图24是图14中底板的结构另一种示意图。
图25是图14中左侧板和左驱动件的示意图。
图26示出实施例三中一种孵育支架的结构示意图。
图27示出实施例三中另一种孵育支架的结构示意图。
图28示出实施例三中又一种孵育支架的结构示意图。
图29示出实施例三中再一种孵育支架的结构示意图。
图30示出实施例四中一种孵育支架的结构示意图。
图31是图30中底板的结构示意图。
图32是图30中盖板与载片贴合或分开过程中的结构示意图。
图33是图30中盖板与载片贴合时的结构示意图。
图34示出实施例五中孵育箱的结构示意图。
图35是图34中孵育机构的孵育支架分离的结构示意图。
图36是图34中壳体机构和驱动机构的结构示意图。
图37是图34中孵育箱在第一视角下的剖面示意图。
图38是图34中孵育机构的支撑组件的分解结构示意图。
图39是图34中支撑组件和孵育支架的结构示意图。
图40是图34中孵育支架的结构示意图。
图41是图34中孵育箱去除后板后的第一种结构示意图。
图42是图34中孵育箱在第二视角下的剖面示意图。
图43是图34中孵育箱去除后板后的第二种结构示意图。
图44示出实施例六染色设备的结构示意图。
图45示出图44中孵育箱与倾斜机构在初始状态下的剖面结构示意图。
图46示出图44中孵育箱与倾斜机构在孵育状态下的剖面结构示意图。
图47是图44中倾斜机构和操作平台的剖面结构示意图。
图48示出实施例六中另一染色设备的结构示意图。
图49示出图48中孵育箱的剖面结构示意图。
图50示出实施例七中一种孵育装置的结构示意图。
图51是图50中孵育装置的分解结构示意图。
图52示出实施例七中另一种孵育装置的结构示意图。
图53示出实施例七中又一种孵育装置的结构示意图。
图54示出本公开实施例中一种孵育支架的结构示意图。
图55是图54中一种孵育支架的部分分解结构示意图。
图56是图54中一种孵育支架在第一角度下的剖面结构示意图。
图57是图54中一种孵育支架在第二角度下的剖面结构示意图。
图58是图54中一种孵育支架去掉一侧侧板的结构示意图。
图59是图58中底板、载片和弧形板的结构示意图。
图60是图58中弧形板的结构示意图。
图61是图54中另一种孵育支架的部分分解结构示意图。
图62是图54中另一种孵育支架在第一角度下的剖面结构示意图。
图63是图54中另一种孵育支架在第二角度下的剖面结构示意图。
图64是图54中另一种孵育支架去掉一侧侧板的结构示意图。
图65是图64中底板、载片和弧形板的结构示意图。
图66是图64中弧形板的结构示意图。
 具体实施方式
现在将参考附图更全面地描述示例实施方式。图中相同的附图标记表示相同或类似的结构，因而将省略它们的详细描述。
实施例一：
参考图1~6，本实施例提供一种孵育支架1，其可以包括支架组件100、一对推动件400以及倾斜设置于支架组件100上的底板200和盖板300。支架组件100包括一对侧板，底板200用于装载载片600，载片600上表面用于设置样本和/或试剂。盖板300设置于底板200上方，载片600位于底板200和盖板300之间。
在实施例中，支架组件100上可以设置多个底板200，每个底板200能够装载一个或多个载片600。以便于同时进行多载片标本的染色和检测，有效提高处理效率。每个底板200对应设置有一个盖板300。
在实施例中，一对推动件400分别滑动连接于一对侧板，底板200和盖板300均连接于一对侧板之间。底板200以固定或可拆卸的方式连接于侧板，盖板300的两侧设有凸起部301，两凸起部301分别与一对推动件400可分离式连接。
侧板上开设有限位槽103，限位槽103设置有第一支撑面104。推动件400上开设有活动槽404，活动槽404设置有第二支撑面405，第一支撑面104和第二支撑面405相互交叉设置，且凸起部301能够支撑在第一支撑面104和第二支撑面405的交叉处，限位槽103用于限制凸起部301沿着第一支撑面104底部至第一支撑面104顶部做往复运动的距离。在实施例中，凸起部301设在盖板300的上部。
参考图1~3、图5和图9，支架组件100包括分别连接于一对侧板之间的第一连接部107和第二连接部108，第一连接部107和第二连接部108分别设置于侧板的前端和末端。第一连接部107和第二连接部108可以是连接板、连接杆等，用于稳固侧板。侧板可以包括对称设置的左侧板101和右侧板102。左侧板101和右侧板102上的限位槽103可以设置成一字形槽。第一支撑面104与装载的载片600的上表面呈交叉设置。
参考图2~5、图8~9，推动件400可以是滑杆或滑板，推动件400包括左右对称设置的左推动件401和右推动件402，左推动件401与左侧板101滑动连接，右推动件402与右侧板102滑动连接。具体的，左侧板101和右侧板102上均开设有滑槽105，左推动件401设置于左侧板101上的滑槽105内。右推动件402设置于右侧板102上的滑槽105内，推动件400能够在滑槽105内往复运动。
在实施例中，滑槽105可以开设在侧板的内侧，并从侧板的一端延伸至另一端。滑槽105与推动件400相适配，左推动件401和右推动件402能同步在滑槽105内朝第一方向A或第二方向B做往复运动。在其他实施例中，滑槽105也可以开设在侧板的外侧。
左侧板101和右侧板102上还开设有导向槽106，导向槽106的开口从侧板顶部延伸至限位槽103，导向槽106与限位槽103连通，凸起部301能够从导向槽106进入或退出限位槽103，便于取放盖板300。
左推动件401、右推动件402上均开设有活动槽404，且两侧的活动槽404左右对称。活动槽404为类似梯形的空间，凸起部301能够在该空间内活动。其中，限位槽103呈一字形，限位槽103的两个平行内壁限制了凸起部301活动，使凸起部301在槽内沿直线运动，始终抵触在第一支撑面104上。凸起部301能够在活动槽404内自由运动，能够脱离第二支撑面405。
参考图6、图8和图10，盖板300的下表面设置有第二隔离部302和支撑台303，支撑台303凸出于盖板300的下表面。第二隔离部302用于将相邻两个载片600上的试剂分隔开来。例如，盖板300为能覆盖三个载片600的规格，盖板300设置有2个第二隔离部302，第二隔离部302可以是长方形凹槽。盖板300与3个载片600贴合的3个接触面之间通过第二隔离部302隔开，防止相邻载片600上的试剂相互流动造成交叉污染。
在实施例中，支撑台303凸出于盖板300的下表面的高度为0.01-2mm。盖板300上每个接触面的四个边角可以分别设置有支撑台303，支撑台303的截面可以是方形、圆形、条形或不规则形等。当盖板300与载片600贴合时，接触面和载片600之间形成毛细缝隙304。当盖板300的上部和载片600逐渐分开时，毛细缝隙304被打开，毛细现象消失，原驻留在毛细缝隙304内的试剂向下流动，并在重力作用下聚集在载片600和盖板300底部的交汇处。当盖板300的上部和载片600逐渐靠拢贴合时，二者之间的毛细缝隙304又重新形成，受到毛细虹吸的作用，底部的试剂又重新从下部逐渐向上部流动，充满毛细缝隙304。
参考图6和图10，盖板300的底部设有倾斜面305，倾斜面305与盖板300的下表面呈角度设置。倾斜面305与盖板300的下表面的夹角为1°至25°，进一步优选为5°至10°，更为优选地，夹角为8°。当载片600与盖板300间具有开口且两者未分离时，底部的倾斜面305与载片600贴合，减少试剂从载片600与盖板300底部流出，避免试剂流失。需要说明的是，倾斜面305与盖板300下表面的夹角也可以为其他角度。
参见图3、图5、图7、图11和图12，底板200包括相互插接的第一底板201和第二底板211，第一底板201的下表面为限位面204，且其两侧设置有第三连接部203，第三连接部203连接于侧板的上部。第二底板211两侧设置有第四连接部212，第四连接部212连接于侧板的底部。盖板300的上部设置于相邻底板的限位面204下方，盖板300能够抵触到限位面204，相邻两个第一底板201之间的间距相同，限位面204用于限制盖板300和载片600之间的开口大小。
盖板300的底部受重力向下垂并与载片600部分贴靠在一起，当凸起部301沿着第一支撑面104底部运动至第一支撑面104顶部时，由于受限位面204的限制，盖板300和载片600之间的开口无法继续增大。盖板300同时受到限位面204向下的压力、第一支撑面104和第二支撑面405向上的支撑力，在这些力的共同作用下迫使盖板300的底部完全翘起，使盖板300与载片600完全分开，这时试剂大部分流失，便于清洗和更换新的试剂。
参考图11，第一底板201的上表面可以设置有容纳槽202和第一隔离部206，第一隔离部206将相邻两个容纳槽202隔开。第一隔离部206使每个容纳槽202相对独立，并隔离相邻载片600，防止相邻载片600上的样本或试剂交叉污染。在实施例中，第一隔离部206在载片600插入口设有导向面205，便于载片600插入容纳槽202内。
参见图2，一对推动件400之间连接有用于与外部的驱动装置连接第五连接部403。通过驱动装置驱动第五连接部403运动，带动一对推动件400同步地向图1所示的第一方向A或第二方向B运动。
参考图7和图13，孵育支架1还可以包括用于托住载片600的限位台500，限位台500设置于底板200的下方，并连接于一对侧板之间，防止载片600从底部掉落。限位台500与载片600接触的位置开设有引流槽501，便于在清洗时进行废液导流。
参见图6和图7，推动件400在外力驱动下向图1所示的第一方向A滑动时，迫使凸起部301沿着第一支撑面104和第二支撑面405的交叉处向上活动，逐渐增大盖板300和载片600之间的开口。如果需要清洗或释放试剂，此时推动件400继续向第一方向A滑动，盖板300的顶部会受到相邻底板200的下表面向下的压力，盖板300的底部会翘起，从而使盖板300顶部和盖板300底部都与载片600分开。
参见图6和图8，推动件400在外力驱动下向图1所示的第二方向B滑动时，迫使凸起部301沿着第一支撑面104和第二支撑面405的交叉处向下活动，逐渐减小盖板300和载片600之间的开口，最终达到盖板300与载片600贴合的状态。如果其中一个盖板被卡住，该盖板的凸起部301脱离与第二支撑面405的配合，并处于活动槽404内的某个位置，其他没有卡住的盖板300继续正常运动。避免因某个盖板300卡住而影响其他盖板的运动。也避免了推动件400继续运动，导致凸起部301断裂的问题。推动件400可以同时带动多个盖板300的运动，且各个盖板300的运动又彼此独立。
在本实施例中，通过推动件400带动盖板300进行活动，使盖板300向载片600逐渐贴合或分开，使得载片600上各个部位的试剂混合更均匀、试剂中检测物与标本中被检测物的结合更快速，有效提高了标本处理的效率和准确性。
实施例二：
参见图14至图18，本实施例提供一种孵育支架1a，包括支架组件100a、一对推动件400a、底板200a和盖板300a，这些组件或部件的连接关系和其它未提及之处可以参照实施例一。
在实施例中，支架组件400a包括一对侧板。一对推动件400a分别滑动连接于一对侧板，底板200a和盖板300a均连接于一对侧板之间，侧板上开设有限位槽103a。盖板300a的两侧设有凸起部301a，两侧凸起部301a分别与一对推动件400a铰接。限位槽103a用于限制凸起部301a往复运动的距离。在实施例中，凸起部301设在盖板300的上部。
支架组件100a包括第一连接部105a和第二连接部106a，侧板包括对称设置的左侧板101a和右侧板102a，左侧板101a、右侧板102a、第一连接部105a和第二连接部106a请参见实施例一中（左侧板101、右侧板102、第一连接部107和第二连接部108）的描述，在此不再赘述。
左侧板101a和右侧板102a上均开设有限位槽103a，底板200a垂直连接于左侧板101a和右侧板102a。限位槽103a中具有两个平行设置的内侧壁107a，内侧壁107a与底板200a上表面呈交叉设置。
参考图14、图16、图22和图24，凸起部301a可以是向外延伸的杆，限位槽103a呈一字形，各个一字形的限位槽103a长度相等且其宽度略大于凸起部301a的直径，能够限制凸起部301a的运动轨迹。
盖板300a上设置有第二隔离部302a、支撑台303a和倾斜面305a，第二隔离部302a、支撑台303a和倾斜面305a请参见实施例一中（第二隔离部302、支撑台303和倾斜面305）的描述，在此不再赘述。
参考图14至图15，推动件400a可以是滑杆或滑板，一对推动件400a包括对称设置的左推动件401a和右推动件402a，左推动件401a与左侧板101a滑动连接，右推动件402a与右侧板102a滑动连接。每个推动件400a与盖板300a一侧的凸起部301a连接。
左推动件401a和右推动件402a的末端之间设置有第四连接部403a，第四连接部403a可以是连接杆或者连接板。第四连接部403a与外部的驱动装置连接，第四连接部403a在驱动装置的驱动下，能够带动左推动件401a和右推动件402a同步地向图14所示的第一方向Aa或第二方向Ba运动。
参考图14、图15和图25，左推动件401a和右推动件402a上均开设有第五连接部404a，第五连接部404a可为通孔，凸起部301a与第五连接部404a铰接。左推动件401a和右推动件402a上的第五连接部404a对称设置，使得各个盖板300a间都互相平行，在推动件400a带动下，多个盖板300a同步运动、步调一致。
参考图14，推动件400a与支架组件100a滑动连接的方式，可以在侧板的内侧开设滑槽104a，推动件400a能够在滑槽104a内沿图14所示的第一方向Aa往复运动，需要说明的是，也可以在侧板的外侧开设滑槽104a。滑槽104a从侧板的一端延伸至侧板的另一端，滑槽104a与推动件400a相适配，左推动件401a和右推动件402a能够同步在滑槽104a内朝图14所示的第一方向Aa或第二方向Ba做滑动。参考图15，不在侧板上开设滑槽104a，推动件400a也可设置在侧板的顶部，推动件400a能够在侧板的顶部往复运动。
参见图14、图16、图23至图25，孵育支架1a上设置有至少两个底板200a，每个底板200a能够装载一个或多个载片500a，以便于同时进行多个载片500a的染色或检测，有效提高处理效率。
底板200a上开设有多个第一隔离部203a和多个容纳槽201a，第一隔离部203a和多个容纳槽201a请参见实施例一中（第一隔离部206和容纳槽202）的描述，在此不再赘述。
参见图23，底板200a可以是一体成型的，底板200a顶端和底端的两侧分别设置有第三连接部202a，底板200a顶端的第三连接部202a分别与左侧板101a和右侧板102a的顶部连接，底板200a底端的第三连接部202a分别与左侧板101a和右侧板102a的底部连接，相邻两个底板200a之间相互平行。
其中，限位台204a设置于底板200a的底部，限位台204a用于托住插入底板200a的载片500a，限位台204a能够防止载片500a从底板200a底部掉落。底板200a的底部开设有引流槽205a，引流槽205a呈镂空状，在清洗盖板300a或载片500a时，污水能够从底板200a底部的引流槽205a中流出。
参见图24，底板200a也可以为可拆卸的，其包括第一底板206a和第二底板207a，则限位台204a设置于支架组件100a的底部，限位台204a两侧分别与左侧板101a和右侧板102a的底部连接，第一底板206a、第二底板207a和限位台204a请参见实施例一中（第一底板201、第二底板211和限位台500）的描述，在此不再赘述。
倾斜设置的底板200a在滴加试剂时，试剂能够流向盖板300a与载片500a的抵接处。在盖板300a和载片500a相对贴合或分开的过程中，盖板300a和载片500a能够共同搅拌试剂，从而使试剂与标本更加快速反应，使得染色或检测的效果更佳。同时，呈倾斜设置的底板200a也有利于在用清水清洗载片500a和盖板300a时，水受重力的作用而倾斜向下流出。
参考图16至图21，盖板300a包括直板或弧形板，直板的下表面为一平面，弧形板下表面为一弧形面。当直板或弧形板与载片500a完全分开前，直板或弧形板始终有部分位置与载片500a贴合。
直板盖板300a上部的凸起部301a受到第五连接部404a以及限位槽103a的支撑，而直板盖板300a下部未与其他部件连接。直板盖板300a下部受重力作用下垂，且其部分位置贴靠在载片500a上。
弧形板盖板300a上部的凸起部301a受到第五连接部404a以及限位槽103a的支撑，而弧形板盖板300a的下部未与其他部件连接。弧形板盖板300a的下部受重力作用下垂，由于弧形板呈弧形，弧形板中部或下部贴靠在载片500a上。
参考图16和图18，盖板300a为直板时，推动件400a在外力驱动下向第二方向Ba滑动：迫使凸起部301a沿限位槽103a的第二端向第一端运动，以减小盖板300a与载片500a之间的开口，最终达到盖板300a贴合于载片500a的状态。
参考图16和图19，盖板300a为直板时，推动件400a在外力驱动下向第一方向Aa滑动：迫使凸起部301a沿限位槽103a的第一端向第二端运动，逐渐增大盖板300a与载片500a之间的开口。如果需要清洗或释放试剂，此时推动件400a继续向第一方向Aa滑动，盖板300a的顶部会受到相邻底板200a的下表面向下的压力，盖板300a的底部会翘起，从而使盖板300a顶部和盖板300a底部都与载片500a分开。
参考图19和图20，盖板300a为弧形板时，推动件400a在外力驱动下向第二方向Ba滑动：迫使凸起部301a沿限位槽103a的第二端向第一端运动，盖板300a的上部与载片500a逐渐贴合，而盖板300a的下部就会以跷跷板的方式做相反的分开运动，最终达到盖板300a上部贴合与载片500a的状态。
参考图19和图21，盖板300a为弧形板时，推动件400a在外力驱动下向第一方向Aa滑动：迫使凸起部301a沿限位槽103a的第一端向第二端运动，盖板300a的上部与载片500a逐渐分开，而盖板300a的下部就会以跷跷板的方式做相反的贴合运动，盖板300a的下部与载片500a逐渐贴合。如果需要清洗或释放试剂，此时推动件400a继续向第一方向Aa滑动，盖板300a的顶部会受到相邻底板200a的下表面向下的压力，盖板300a的底部会翘起，从而使盖板300a顶部和盖板300a底部都与载片500a分开。
在本实施例中，如果采用直板盖板300a，当盖板300a的上部和下部都与载片500a贴合时，所形成的毛细缝隙304a将较全面地覆盖载片500a，试剂将受毛细虹吸的作用充满毛细缝隙304a，这样有利于对载片500a上较大范围的标本进行染色与检测。
如果采用弧形板盖板300a，当盖板300a上部与载片500a分开或贴合时，弧形板的下部就会以跷跷板的方式相反地与载片500a贴合或分开，盖板300a与载片500a之间始终只有部分区域贴合，因此只有部分区域形成毛细缝隙304a，并具备虹吸试剂充满的毛细缝隙304a。采用弧形板更有利于促进载片500a上试剂大幅度的往复运动，更加强化动态孵育的效果，孵育时间可以更加缩短，检测灵敏度较高。并且通过外力控制推拉组件往复运动的距离，可以控制弧形板与载片500a贴合的范围，从而缩小载片500a上试剂覆盖的区域，减少试剂的使用量，尤其是在进行如荧光原位杂交或使用其它昂贵试剂时，能节省成本。
实施例三：
参考图26至图28，本实施例提供一种孵育支架1b，其可以包括支架组件100b、底板200b、盖板300b和推动件400b，这三者之间与载片的装配关系参照实施例一。其中，盖板300b能够相对底板200b运动，盖板300b贴合在载片600b上滑动，以混匀盖板300b与载片600b之间的试剂。
支架组件100b包括侧板、第一连接部105b和第二连接部106b，侧板包括对称设置的左侧板101b和右侧板102b，左侧板101b、右侧板102b、第一连接部105b和第二连接部106b请参见实施例一中（左侧板101、右侧板102、第一连接部107和第二连接部108）的描述，在此不再赘述。
侧板上开设有第一限位槽103b，第一限位槽103b中具有两个平行设置的内侧壁104b。盖板300b两侧设置有凸起部301b，凸起部301b在第一限位槽103b内滑动。两侧凸起部301b分别与一对推动件400b铰接，盖板300b与载片600b贴合设置。
底板200b可以为一体成型，底板200b的结构请参见实施例二中的描述，在此不再赘述。
左侧板101b和右侧板102b中均开设有第一限位槽103b，第一限位槽103b可以设置成一字型槽。第一限位槽103b中长度相等且宽度略大于凸起部301b直径，能够限制凸起部301b的运动轨迹。第一限位槽103b中具有两个平行设置的内侧壁104b，内侧壁104b与底板200b上表面呈平行设置。其中，第一限位槽103b的宽度指的是两个平行设置的内侧壁104b之间的距离。每个第一限位槽103b中第一端比第二端靠近底板200b的顶部。
第一限位槽103b中位于下方的内侧壁104b与底板200b上表面之间的距离略大于载片500b的厚度，这样能够使载片500b位于底板200b上表面与盖板300b下表面之间。
凸起部301b可以是向外延伸的杆，盖板300b为直板，盖板300b可参见实施例一中（盖板300）的描述，在此不再赘述。盖板300b上设置有第二隔离部、支撑台303b、毛细缝隙304b和倾斜面，第二隔离部、支撑台和倾斜面请参见实施例一中（第二隔离部302、支撑台303、毛细缝隙304和倾斜面305）的描述，在此不再赘述。
推动件400b可以是滑杆或滑板，一对推动件400b包括左右对称设置的左推动件401b和右推动件402b，左推动件401b与左侧板101b滑动连接，右推动件402b与右侧板102b滑动连接。其中，推动件400b与支架组件100b滑动连接的方式，可以是在侧板的外侧或者内侧开设滑槽105b，推动件400b能够在滑槽105b内往复运动。
参考图28，推动件400b上设置有第二限位槽403b，第二限位槽403b连接盖板300b的凸起部301b，一对推动件400b之间设置有第四连接部404b。第四连接部404b可以是连接杆或者连接板，通过螺丝连接于左推动件401b和右推动件402b的同一端。第四连接部404b能够与外部的驱动装置连接，第四连接部404b在驱动装置的驱动下，能够带动左推动件401b和右推动件402b同步地向图26所示的第一方向Ab或第二方向Bb运动。
参见图26，第二限位槽403b可以是一字形限位槽，第二限位槽403b的宽度略大于凸起部301b的直径，凸起部301b能够在第二限位槽403b中活动。参考图29，第二限位槽403b也可以设置成一个圆孔，圆孔的直径略大于凸起部301b的直径，凸起部301b能够在圆孔转动。
参考图26至图29，第一限位槽103b的长度方向与第二限位槽403b的长度方向呈交叉设置，凸起部301b同时位于对应的第一限位槽103b和第二限位槽403b中。其中，第一限位槽103b中第一端比第二端靠近底板200b的顶部，其第二端比第一端靠近底板200b的底部。
第二限位槽403b垂直或者倾斜设置，第二限位槽403b的第一端比第二端靠近支架组件100b的顶部，其第二端比第一端靠近支架组件100b的底部。当然，第二限位槽403b还可以水平设置，此时，第二限位槽403b中第一端比第二端靠近第一连接部106b，其第二端比第一端靠近第二连接部107b。
当推动件400b向第一方向Ab滑动时：第二限位槽403b的内侧壁104b推动凸起部301b，使凸起部301b沿着第一限位槽103b从第一端向第二端方向运动。盖板300b贴在载片500b向下滑动，直至凸起部301b运动至第一限位槽103b的第二端和/或第二限位槽403b的第二端时停止滑动。
当推动件400b向第二方向Bb滑动时：第二限位槽403b的内侧壁104b推动凸起部301b，使凸起部301b沿着第一限位槽103b从第二端向第一端方向运动。盖板300b贴在载片500b向上滑动，直至凸起部301b运动至第一限位槽103b的第一端和/或第二限位槽403b的第一端时停止滑动。
在本实施例中，通过推动件400b带动盖板300b进行活动，使得与载片600b贴合的盖板300b能够沿载片600b的长度方向往复滑动，在此过程中，处于盖板300b和载片600b之间的标本和试剂起到搅拌混匀效果，让标本和试剂快速充分反应，避免试剂浓度不均和气泡聚集的情况出现以及较长时间的静置等待，进而提高载片上标本处理的效率和准确性。需要强调的是，盖板300b始终贴合载片500b的上表面滑动。
实施例四：
参考图30至图33，本实施例提供一种孵育支架1c，其可以包括支架组件100c、底板200c和盖板300c，底板200c用于装载载片400c，载片400c位于底板200c和盖板300c之间。
在实施例中，支架组件100c包括上层支架101c和下层支架104c，二者可以采用板状结构。底板200c上部的两侧与上层支架101c连接，底板200c下部的两侧与下层支架104c连接。盖板300c对应设置于底板200c一侧，且盖板300c上部的两侧与上层支架101c连接，盖板300c的下部能够贴靠在载片400c的上表面。上层支架101c在外力的驱动下能够相对于下层支架104c发生移动，使得上层支架101c与下层支架104c之间逐渐相对分开或靠拢，以增大或减小盖板300c与载片400c之间的开口大小。
在实施例中，上层支架101c以弧形轨迹运动且相对于下层支架104c逐渐分开和靠拢，使得与上层支架101c连接的盖板300c运动幅度较大，有利于盖板300c与载片400c之间的试剂流动、消除试剂中的气泡。
上层支架101c包括第一上支架102c和第二上支架103c，第一上支架102c和第二上支架103c是左右对称设置。第一上支架102c和第二上支架103c相互平行。第一上支架102c和第二上支架103c均开设有多个第一连接部108c和第二连接部109c。
下层支架104c可以包括第一下支架105c和第二下支架106c，第一下支架105c和第二下支架106c是左右对称设置。第一下支架105c和第二下支架106c相互平行设置。第一下支架105c和第二下支架106c均开设有多个第一连接部108c。相邻两个第一连接部108c的距离相等，相邻两个第二连接部109c的距离相等。
底板200c的两侧在顶部和底部位置均设有第三连接部202c。第三连接部202c可以是凸起的轴杆，第一连接部108c可以是圆孔，第三连接部202c能够在第一连接部108c中转动。通过第三连接部202c与第一连接部108c的连接，使底板200c转动连接于第一上支架102c和第二上支架103c之间，以及转动连接于第一下支架105c和第二下支架106c之间。
盖板300c两侧设置有凸起部301c，凸起部301c位于盖板300c的上部。凸起部301c与第二连接部109c转动连接，盖板300c通过凸起部301c连接于第一上支架102c和第二上支架103c之间。由于，盖板300c的上部有支撑力，而盖板300c的下部没有与下层支架104c连接，盖板300c下部受重力作用向下垂，并能够贴靠在载片400c上表面或者是悬空。
盖板300c上还设置有支撑台303c、倾斜面305c、毛细缝隙304c和第二隔离部，支撑台303c、倾斜面305c、毛细缝隙304c和第二隔离部请参见实施例一中（支撑台303、倾斜面305、毛细缝隙304和第二隔离部302）的描述，在此不再赘述。
支架组件100c还可包括多个连接件107c，连接件107c可以是连接杆。在上层支架101c中，连接件107c可以设置于第一上支架102c和第二上支架103c的两端。在下层支架104c中，连接件107c可以设置于第一下支架105c和第二下支架106c的两端和中间位置。
参考图31，底板200c为一体成型，底板200c设置有容纳槽201c、第一隔离部203c、限位台204c和引流槽205c，容纳槽201c、第一隔离部203c、限位台204c和引流槽205c请参见实施例二中（一体成型的底板200a中容纳槽201a、第一隔离部203a、限位台204a和引流槽205a）的描述，在此不再赘述。
当上层支架101c在外力的驱动下相对于下层支架104c向图30所示的第一方向Ac移动：上层支架101c与下层支架104c逐渐分离，使得盖板300c与载片400c之间的开口逐渐增大。当需要进行清洗和/或更换试剂时，上层支架101c继续运动，直至盖板300c与下层支架104c之间的夹角接近、大于或等于90°时，盖板300c与载片400c完全分离，盖板300c底部与载片400c下部的交汇处消失，试剂或者清洗液体能够从盖板300c和载片400c之间流走。
当上层支架101c在外力的驱动下相对于下层支架104c向图30所示的第二方向Bc移动时：上层支架101c与下层支架104c逐渐靠拢，使得盖板300c与载片400c之间的开口逐渐减小，直至盖板300c与载片400c达到贴合状态。
通过上层支架101c与外部驱动装置连接，在外部驱动装置的驱动下，上层支架101c能够相对于下层支架104c沿第一方向Ac或第二方向Bc发生移动。在上层支架101c和下层支架104c逐渐分离的过程中，将带动盖板300c上部与载片400c上部分离，毛细缝隙304c被打开，毛细缝隙304c的试剂由于重力的作用流向盖板300c底部与载片400c之间的夹角处。
在上层支架101c和下层支架104c逐渐靠拢的过程中，盖板300c上部和载片400c上部将重新贴合重新形成毛细缝隙304c，聚集在盖板300c底部和载片400c下部夹角之间的试剂由于毛细虹吸的作用，又重新扩散至形成的毛细缝隙304c内。如此循环往复，使得试剂在盖板300c与载片400c之间处于流动状态，起到搅拌均匀、消除气泡、试剂全面覆盖标本的动态孵育效果，试剂中检测物与标本中的被检测物的结合更加快速、非特异性结合更少，有效提高了载片400c标本处理的质量和效率。
实施例五：
参考图34~36，本公开提供一种孵育箱1d，该孵育箱1d包括壳体机构100d、驱动机构200d和孵育机构300d。壳体机构100d内设有安装腔，驱动机构200d和孵育机构300d设置于安装腔，驱动机构200d能够驱动孵育机构300d中的盖板343d相对于载片345d运动，以混匀盖板343d与载片345d之间的试剂，实现动态孵育，提高载片345d上标本处理的效率和质量。
孵育机构300d包括支撑组件310d和孵育支架340d，支撑组件310d与安装腔的内壁连接，支撑组件310d与安装腔的内壁以可推拉的方式活动连接。支撑组件310d包括设置有孵育槽312d的孵育锅311d，孵育支架340d可移出地设置于孵育槽312d，支撑组件310d能够带动孵育支架340d前后运动。孵育支架340d包括盖板343d和载片345d。孵育支架340d请参见上述实施例一至四中的描述，在此不再赘述。
通过设置支撑组件310d与安装腔的内壁为可推拉的方式活动连接，使支撑组件310d相对于壳体机构100d具有拉开和合上的功能，即支撑组件310d能够像抽屉一样安装于壳体机构100d内，且孵育支架340d与孵育槽312d为可拆卸连接，这样便于孵育支架340d与孵育锅311d的拆装。
在本实施例中，驱动机构200d包括驱动件210d和从动件220d，驱动件210d驱动连接于从动件220d。从动件220d与盖板343d传动连接，并带动盖板343d相对于载片345d运动。
参考图34至图36，壳体机构100d可以包括前板110d、后板120d、第一侧板130d、第二侧板140d、上盖150d和底座板160d。通过前板110d、后板120d、第一侧板130d、第二侧板140d、上盖150d和底座板160d连接形成有一安装腔，该安装腔内可以设置有风扇161d。上盖150d与第一侧板130d铰接，上盖150d可以打开。前板110d设有让孵育机构300d进出的开口。
需要说明的是，上盖150d与第一侧板130d的连接方式，并不局限于可翻转连接，也可以是固定连接。例如，上盖150d的一边缘与第一侧板130d固定连接，且上盖150d可以跟百叶窗的结构一样，具有打开和收拢的功能，这样也能够实现上盖150d的开合。
当壳体机构100d内需要降温时，可以通过风扇161d来实现。另外，还可以在底座板160d和后板120d上开设有散热孔，用来进行降温，以保护壳体结构内的电子元器件。
参考图40，在一示例中，在上盖150d上开设有多个滴液孔151d，滴液孔151d为通孔，上盖150d位于孵育锅311d的上方。其中，孵育锅311d中能够装载孵育支架340d，孵育支架340d包括盖板343d和载片345d，多个滴液孔151d与下方的多个载片345d一一对应，且滴液孔151d的正下方对准载片345d的上部，试剂从滴液孔151d滴落在载片345d与盖板343d之间。需要说明的是，上盖150d也可以不开设有滴液孔151d，滴加试剂时，可以通过打开上盖150d并将试剂滴加在载片345d与盖板343d之间。
参考图35至图38，支撑组件310d可以包括支撑架315d、一对滑轨和孵育锅311d，一对滑轨分别连接于支撑架315d的两侧，孵育锅311d连接在支撑架315d上，且孵育锅311d内开设有孵育槽312d，孵育槽312d能够装载孵育支架340d。
支撑架315d可以包括第一支撑板316d、第二支撑板317d、第三支撑板318d和横梁319d，第三支撑板318d设置于横梁319d的前方，且第三支撑板318d的两端分别连接在第一支撑板316d和第二支撑板317d的前端，横梁319d的两端分别连接在第一支撑板316d和第二支撑板317d的后端，且横梁319d用于支撑孵育锅311d的底部。
其中，孵育锅311d与支撑架315d的连接方式可以为固定连接，也可以为支撑连接。例如，可以通过螺母和螺栓，将孵育锅311d的四周分别与第一支撑板316d、第二支撑板317d、第三支撑板318d和横梁319d进行固定连接。又例如，在第一支撑板316d、第二支撑板317d和第三支撑板318d的上边缘开设有台阶槽，让孵育锅311d的锅体上边缘能够支撑在台阶槽中，而孵育锅311d底部支撑在横梁319d上，这样可以在需要更换孵育锅311d时更加便捷。
一对滑轨可以包括第一滑轨321d和第二滑轨322d，第一支撑板316d和第一侧板130d连接在第一滑轨321d的两侧，第二支撑板317d和第二侧板140d连接在第二滑轨322d的两侧，通过拉动第三支撑板318d的前后运动，支撑架315d能够在第一滑轨321d和第二滑轨322d的引导下前后移动。
支撑架315d通过一对滑轨滑动连接于安装腔的侧壁，且孵育锅311d连接在支撑架315d上，这样能够使支撑架315d带动孵育锅311d从安装腔内滑出，进而带动孵育支架340d前后活动，便于从孵育槽312d内取出孵育支架340d，能够方便对组织样本装载以及对孵育支架340d进行清洗。
在另一实施例中，支撑组件310d与安装腔的内壁还可以是其它连接方式。例如，支撑组件310d与安装腔的内壁可以是固定连接。具体的，支撑组件310d可以包括支撑架315d和孵育锅311d，支撑架315d固定连接于安装腔的两侧内壁，孵育锅311d与支撑架315d连接，壳体机构100d的上盖150d与第一侧板130d可翻转连接，上盖150d能够相对于第二侧板140d做开合运动，在上盖150d打开状态下，便于将孵育支架340d从孵育锅311d中取出。
支撑组件310d还可以包括前面板320d，可以在前面板320d上设置有显示屏，该显示屏可以为触摸屏，不仅可以显示孵育箱1d的工作状态信息，也能够在改显示屏上进行控制操作。
支撑组件310d还可以包括加热件323d和隔热件324d，加热件323d连接于孵育锅311d的底部，并固定连接在支撑架315d上。加热件323d用于对孵育锅311d的锅体加热，进而能够对孵育槽312d内的水进行加热处理。隔热件324d设置于加热件323d的下方并连接于支撑架315d的底部，隔离板主要起到隔热保护作用，避免加热件323d在加热过程中，温度过高而损害安装腔内的其他电子元器件。需要说明的是，加热件323d可以是加热片、加热膜等加热器件，隔热件324d可以是隔热板。加热也可以是在外在的加热并通过管道输送进孵育锅内。
参考图35至图38，在实施例中，支撑组件310d包括多个孵育锅311d，孵育锅311d并排设置。每个孵育锅311d上均开设有孵育槽312d，每个孵育槽312d内能够装载至少一个孵育支架340d。
参考图38至图40，孵育槽312d的底部设置有限位柱313d，孵育支架340d的底部设置有限位槽346d，限位柱313d能够与限位槽346d配合，用以限制孵育支架340d晃动。
孵育支架340d包括支架组件341d、推拉组件342d、盖板343d、底板344d和载片345d，通过推拉组件342d的前后推动，盖板343d能够相对于载片345d运动，这样能够搅动盖板343d与载片345d之间的试剂，使试剂加速与载片345d上的组织样本反应。具体可参见实施例一至四任意一个结构的描述。
支架组件341d可以包括一对侧板，两个侧板的底部开设有限位槽346d，每个侧板上可以开设有两个限位槽346d，限位槽346d呈半圆柱形。相应的，在孵育槽312d的底部上设置有多个限位柱313d，限位柱313d能够与限位槽346d一一对应，限位槽346d与限位柱313d相适配，限位柱313d能够插入限位槽346d中，并通过两个或两个以上的限位柱313d来限制孵育支架340d前后、左右的晃动。其中，从左至右的方向上，相邻两个限位柱313d之间的距离，或者限位柱313d与孵育槽312d侧边的距离均略大于孵育支架340d的宽度。从前至后的方向上，相邻两个限位柱313d之间的距离等于孵育支架340d同一侧上两个限位槽346d之间的距离。
推拉组件342d可以包括一对推动件和连接部，推动件和连接部请参见实施例一至三中（例如，推动件400和第五连接部403等）的描述，在此不再赘述。或者，推拉组件342d包括上层支架和连接部，上层支架和连接部请参见实施例四中（上层支架101c和连接件107c）的描述，在此不在赘述。
参考图39和图41，孵育槽312d的后侧壁上开设有进水孔335d，在孵育锅311d的后侧设置有进水接头336d，进水孔335d与进水接头336d连通，进水接头336d与进水泵连接。孵育槽312d的底部上开设有出水孔337d，孵育锅311d的底部设置有出水接头338d，出水孔337d与出水接头338d连通，出水接头338d与出水泵连接。
参考图38至图40，驱动机构200d传动连接于孵育支架340d，通过驱动件210d驱动从动件220d运动，以带动盖板343d相对于载片345d运动。
支撑组件310d还包括连接组件330d，连接组件330d包括第一推板331d、第二推板332d、连接杆333d和弹性件334d。其中，第二推板332d可以由磁铁制成的板状物，推拉组件342d上末端的连接部也可以是由铁、镍、钴等能够被磁铁吸附的材料制成，弹性件334d可以是弹簧等。
第一推板331d和第二推板332d分别连接在连接杆333d的两端，在孵育锅311d的后侧且对应第二推板332d的位置上开设有连杆孔314d，连杆孔314d为一圆形通孔，连接杆333d能够穿过连杆孔314d并在连杆孔314d中前后活动。第一推板331d和第二推板332d分别连接在连接杆333d的两端，第二推板332d位于孵育槽312d内，弹性件334d套设在连接杆333d上并位于孵育锅311d与第一推板331d之间。
通过将弹性件334d套设在连接杆333d上并位于孵育锅311d和第一推板331d之间，第一推板331d和第二推板332d分别连接在连接杆333d的两端，这样能够实现第一推板331d和第二推板332d的同步运动，并具有恢复原始状态的功能。当从动件220d向前运动并向前抵压第一推板331d，这样能够带动第二推板332d向前运动且弹性件334d被压缩。当从动件220d向后运动时，弹性件334d有恢复原状的趋势，此时弹性件334d对第一推板331d施加向后的推力，使得弹性件334d推动第一推板331d向后运动，并带动第二推板332d向后运动至原始状态的位置。
其中，从动件220d抵接在第一推板331d上，且第二推板332d传动连接于盖板343d。第二推板332d能够靠磁力，将推拉组件342d末端的连接部吸附在一起，通过从动件220d驱动第一推板331d的前后运动，以带动第二推板332d的前后运动，第二推板332d带动连接部前后运动，连接部带动推动件前后运动，推动件带动盖板343d相对载片345d运动。第一推板331d与从动件220d为可分离式连接，当支撑组件310d从安装腔内拉出的过程中，驱动组件并不会跟随连接组件330d向前运动，这样能够避免驱动组件上的电线被拉出导致电线受损。
参考图35、图39至图42，驱动件210d可以为丝杆电机，从动件220d可以为传动杆或传动板。驱动件210d的输出端与从动件220d的下端铰接，且驱动件210d的输出端能够前后移动。壳体机构100d还可以包括安装板170d，从动件220d的中部与安装板170d铰接，使得从动件220d上下端能够以与安装板170d铰接的位置为轴而摆动，当驱动件210d输出端的向前移动时，从动件220d的上端向后摆，当驱动件210d输出端向后移动时，从动件220d的上端向前摆。这样通过从动件220d上端的前后摆动，从而使第一推板331d向前和向后移动，进而带动盖板343d相对于载片345d运动。
其中，驱动件210d与底座板160d固定连接，并位于孵育机构300d的下方，且驱动件210d位于孵育机构300d俯视投影的范围内。使得连接组件330d、从动件220d和驱动件210d呈U字形排列，这样能够缩小壳体机构100d的前后距离以及其占地面积，避免连接组件330d、从动件220d和驱动件210d呈一字形排列，导致壳体机构100d前后距离较大而占用空间。需要说明的是，驱动件210d位于孵育机构300d俯视投影的范围内，但本公开并不局限于此，驱动件210d也可以设置在孵育机构300d的后侧。
参考图39、图40和图43，驱动件210d可以为步进电机，从动件220d可以为偏心轮。壳体机构100d还可以包括安装板170d，驱动件210d以竖直的方式与安装板170d固定连接，驱动件210d的输出端朝上并与偏心轮的底端连接，在驱动件210d的输出端转动时偏心轮也跟着转动。偏心轮的周缘抵接在第一推板331d上，偏心轮转动会带动第一推板331d前后运动，从而使盖板343d相对于载片345d运动。其中，驱动件210d和从动件220d位于连接组件330d的下方，且不完全在连接组件330d的俯视投影的范围内。驱动件210d采用步进电机，相对于丝杆电机而言能够更加节省成本。
虽然上述实施例中，驱动件210d和从动件220d位于连接组件330d下方，但本公开并不局限于此。驱动件210d和从动件220d也可以位于连接组件330d的后方，例如，驱动件210d可以采用步进电机，从动件220d可以为从动杆，步进电机固定连接于安装板170d的顶部，步进电机的输出端连接从动杆，从动杆抵接在第一推板331d上。此时，从动杆和步进电机的输出轴成一条直线，且垂直于第一推板331d上，通过步进电机的输出轴前后运动，使得第一推板331d能够前后运动。
实施例六：
参考图44~49，本实施例提供一种染色设备，包括机台100e和设置在机台100e上孵育箱200e和倾斜机构300e。倾斜机构300e驱动孵育箱200e向下摆动，使盖板300和载片600相对于水平面Ae的倾斜角Be变小，减少盖板300与载片600间的试剂流失。
孵育箱200e可以包括壳体机构210e、孵育锅220e和至少一组孵育支架，孵育锅220e设置在壳体机构210e上，孵育支架可移出地设置于孵育锅220e上。壳体机构210e、孵育锅220e可采用实施例五中的结构，孵育支架可采用实施例一至四的孵育支架1、1a、1b和1c中的结构，在此不再赘述。下述以孵育支架采用实施例一中结构为例，进行描述。
位于初始状态时，孵育箱200e、孵育锅220e、支架组件100与水平面Ae保持平行。底板、盖板和载片相对支架组件倾斜设置，即底板、盖板和载片与水平面具有一定夹角。底板的倾斜角Be可以为15°至90°，对应的，载片600的倾斜角Be为15°至90°。例如，初始状态下，选用底板倾斜角Be为35°的孵育支架，，载片600的倾斜角Be也为35°。
当孵育箱200e向下摆动至孵育状态时，孵育箱200e与水平面Ae呈一定夹角，而底板、载片和盖板与水平面Ae的夹角变小。孵育状态下的载片600与水平面Ae的倾斜角Be，小于初始状态下的载片600与水平面Ae的倾斜角Be。例如，可选择底板200倾斜角Be为35°的孵育支架，初始状态时，载片600相对于水平面Ae的倾斜角Be为35°。孵育箱200e摆动至孵育状态，载片600与水平面Ae的倾斜角Be为0°（即载片600与水平面Ae平行）。需说明的是，孵育状态下，载片600与水平面Ae的倾斜角Be也可以为2°、3°、4°等。
参考图49，孵育箱200e包括驱动组件，盖板300在驱动组件的驱动下能够相对载片600运动。驱动组件可包括驱动电机241e和连接板242e，驱动电机241e可设置于孵育箱200e内，连接板242e一端与驱动电机241e的输出端连接，另一端与推动件400连接。驱动电机241e驱动连接板242e前后运动，以带动推动件400在滑槽105内前后滑动。驱动电机241e和连接板242e请参见实施例五中的描述，在此不再赘述。
参考图44至图47，倾斜机构300e包括托板320e和驱动件310e，托板320e的两侧与机台100e铰接，驱动件310e设在机台100e上，且与托板320e的底部连接，以驱动托板320e摆动，孵育箱200e设置在托板320e上。
机台100e上设置有操作平台110e，操作平台110e上开设有通槽111e，通槽111e可以为正方形、长方形等，其形状与托板320e相适配。在通槽111e两侧边缘上设有一对向下延伸的支撑板120e，托板320e铰接设置于一对支撑板120e之间。支撑板120e和托板320e在铰接点处开设有轴孔，并通过连轴件122e（可以是连轴螺栓、轴杆等）连接，托板320e绕连轴件122e摆动。初始状态下，托板320e与水平面Ae平行，孵育状态下，托板320e与水平面Ae呈一定夹角。
支撑板120e上在远离铰接点处的位置开设有弧形槽121e，该弧形槽的圆心与铰接点重合。托板320e的两侧设置有向外凸起的导杆321e，导杆321e能在弧形槽121e内滑动。其中，弧形槽121e的弧形轨迹长度约为23厘米，进而托板320e向下摆动的弧形轨迹的最大距离可以为23厘米。
倾斜机构300e包括底座330e，底座330e设置于机台100e内，驱动件310e的两端分别与底座330e和托板320e铰接。在一示例中，驱动件310e为电动推杆，驱动件310e的顶端与三角连接架340e的尖端铰接，该三角连接架340e的平面端与托板320e的底面连接。驱动件310e的底端与三角连接架340e的尖端铰接，该三角连接架340e的平面端与底座330e的顶面连接，通过驱动件310e顶端和底端铰接的方式，能够更好的配合托板320e摆动。
在本实施例中，驱动件310e是连接在托板320e的底部，但本公开并不局限于此，例如，驱动件310e可以是减速步进电机或舵机，减速步进电机或舵机设置在支撑板120e和托板320e的铰接点处，并与铰接点处的连轴件122e驱动连接（图未示），减速步进电机或舵机驱动连轴件122e转动，以调整托板320e与水平面Ae的倾斜度。
参考图48，操作平台110e上设置有机械臂400e试剂瓶420e，，机械臂400e可以沿前后、左右、上下方位移动。机械臂400e上连接有加样针410e，机械臂400e带动加样针410e在操作平台110e上方自由移动。当机械臂400e能够将加样针410e移动至试剂瓶420e内，抽取试剂瓶420e内的试剂。并移动至盖板300与载片600上方，以将试剂滴入盖板300与载片600的缝隙304中。
参考图44和图49，孵育箱200e的安装腔内设置有注水装置（图未示）和排液装置（图未示），且孵育锅220e开设有注水口和排液口，注水装置与注水口连接，排液装置与排液口连接。操作平台110e下方还可以设置用于装清水的水箱和用于收集废液的废液箱。注水装置能够将水箱中的清水抽取到孵育锅220e内，排液装置能够将孵育锅220e内废液抽取到废液箱中。
参考图48，机台100e的一侧设有上位机，上位机分别与孵育箱200e、机械臂400e和倾斜机构300e电连接，上位机可以是电脑，以控制孵育箱200e、机械臂400e和倾斜机构300e的工作状态。
本实施例提供一种染色方法，能够应用在上述的染色设备上，该方法可以包括：
步骤S102，向盖板300与载片600之间的缝隙304添加液体。具体地，此时，孵育箱200e处于初始状态，孵育箱200e、托板320e与水平面Ae平行。与水平面Ae保持平行时，
步骤S104，倾斜机构300e驱动孵育箱200e向下摆动至孵育状态，倾斜机构300e停止驱动。具体地，在孵育箱200e向下摆动过程中，孵育箱200e与水平面Ae的倾斜角Be变大，而盖板300和载片600与水平面Ae的倾斜角Be变小。例如，选择底板200倾斜角Be为35°的孵育支架，以及设定孵育状态时载片600与水平面Ae的倾斜角Be为0°。驱动件310e驱动孵育箱200e向下摆动至孵育状，载片600与水平面Ae的倾斜角Be由35°变换成0°。
步骤S106，盖板300相对载片600运动，以使盖板与载片间的液体混匀。具体地，孵育箱200e处于孵育状态时，驱动电机241e驱动推动件400在滑槽105内前后运动，以带动盖板相对载片运动，使得盖板与载片间的试剂与标本充分反应，或者使盖板与载片间的残留物与清水充分搅动清洗。
可以理解的是，盖板相对载片的运动方式根据采用实施例一至实施例四中的孵育支架的结构不同而不同，例如板相对载片开合运动和贴合运动。开合运动为盖板的底部抵接在载片上，而盖板的顶部做相对于载片靠近和远离的运动；贴合运动为盖板与载片贴合，且盖板沿载片长度方向做往复运动。
步骤S108，倾斜机构300e驱动孵育箱200e向上摆动，且摆动至孵育箱200e与水平面Ae平行时，倾斜机构300e停止驱动。具体地，驱动件310e驱动托板320e向上摆动，以使孵育箱200e由孵育状态变成初始状态。参见步骤S104中的示例，孵育箱200e由孵育状态变成初始状态的过程中，载片与水平面Ae的倾斜角Be由0°变换成35°。
需要说明的是，该染色方法可以应用于载片标本的检测，包括脱蜡、清洗、修复、一抗试剂添加、阻断剂添加、二抗试剂添加、显色剂添加、苏木素添加和返蓝的处理过程的所有步骤或个别步骤。
本实施例中，通过倾斜机构300e驱动孵育箱200e向下摆动，以使盖板和载片相对于水平面Ae的倾斜角Be变小。能够避免在孵育过程中盖板与载片的倾斜角Be过大，而导致盖板与载片之间的试剂从缝隙底部流失的风险，节约试剂用量，无需多次添加试剂，提高检测效率。
实施例七：
参考图50至图53，本公开提供一种孵育装置，包括基座100f、孵育支架200f和倾斜机构300f，倾斜机构300f设置在基座100f上，并与孵育支架200f驱动连接。
参考图50和图51，在一示例中，倾斜机构300f和孵育机构分别设置在基座100f上，驱动机构400f与倾斜机构300f连接。其中，基座100f可以包括底座110f和两个对称设置的支撑柱120f，支撑柱120f设置在基座100f的左端，而驱动机构400f设置在远离支撑柱120f的一端，孵育支架200f的尾部朝向驱动机构400f。
倾斜机构300f包括第一驱动件320f和托板310f，托板310f的一端与两个支撑柱120f铰接。例如，两个支撑柱120f上分别设置有轴杆121f，两个轴杆121f对称设置，在托板310f上对应轴杆121f的位置开设有通孔，可以通过轴杆121f和通孔实现托板310f与两个支撑柱120f的铰接。第一驱动件320f可以为伸缩电机，伸缩电机的输出端与托板310f的一端（远离支撑柱120f的一端）连接，伸缩电机的底部与底座110f连接。
驱动机构400f连接在托板310f的一端，驱动机构400f可以包括第三驱动件410f和连接块420f，第三驱动件410f可以为偏心轮电机，第三驱动件410f与连接块420f连接，连接块420f与盖板220f间接连接。当第三驱动件410f驱动连接块420f前后活动时，连接块420f能够带动盖板220f相对载片230f运动，以加快标本与试剂的反应速度，提高检测效率。
该孵育装置的工作过程可以是：初始状态时，托板310f与水平面保持平行，托板310f受两个支撑柱120f和第一驱动件320f的支撑。此时，可以向盖板220f与载片230f之间的缝隙添加液体。当初始状态要转换成孵育状态时，第一驱动件320f的输出端缩收，托板310f沿轴杆121f向下摆动，对应的，孵育支架200f同步向下摆动。孵育支架200f向下摆动至孵育状态时，第一驱动件320f停止驱动。此时，载片230f与水平面的倾斜角变小，以降低盖板220f与载片230f之间的试剂从缝隙底部流失的风险。第一驱动件320f停止驱动后，可以启动驱动机构400f工作，使盖板220f相对载片230f运动，以使盖板220f与载片230f间的标本和试剂混匀。当混匀结束后，驱动机构400f停机工作，第一驱动件320f的输出端伸出，以驱动孵育支架200f向上摆动，且摆动至托板310f与水平面平行时，第一驱动件320f停止驱动，此时孵育支架200f与水平面平行。
参考图52，在另一示例中，倾斜机构300f和孵育机构分别设置在基座100f上，驱动机构400f与倾斜机构300f连接。其中，基座100f可以包括底座110f和两个对称设置的支撑柱120f，支撑柱120f设置在基座100f的左端，且驱动机构400f也设置靠近支撑柱120f的一端，孵育支架200f的尾部朝向驱动机构400f。
倾斜机构300f可以包括第一驱动件320f和托板310f，托板310f的一端与两个支撑柱120f铰接。例如，两个支撑柱120f上分别设置有轴杆121f，两个轴杆121f对称设置，在托板310f上对应轴杆121f的位置开设有通孔，可以通过轴杆121f和通孔实现托板310f与两个支撑柱120f的铰接。第一驱动件320f可以为伸缩电机，伸缩电机的输出端与托板310f远离支撑柱120f的一端连接，伸缩电机的底部与底座110f连接。
驱动机构400f连接在托板310f的一端，驱动机构400f可以包括第三驱动件410f和连接块420f，第三驱动件410f可以为偏心轮电机，第三驱动件410f与连接块420f连接，连接块420f与盖板间接连接（具体连接方式请参考下文的孵育支架200f介绍）。当第三驱动件410f驱动连接块420f前后活动时，连接块420f能够带动盖板相对载片运动，以加快标本与试剂的反应速度，提供检测效率。
该孵育装置的工作过程可以是：初始状态时，托板310f与水平面保持平行，托板310f受两个支撑柱120f和第一驱动件320f的支撑。此时，可以向盖板与载片之间的缝隙添加液体。当初始状态要转换成孵育状态时，第一驱动件320f的输出端伸出，托板310f沿轴杆121f向上摆动，对应的，孵育支架200f同步向上摆动。孵育支架200f向上摆动至孵育状态时，第一驱动件320f停止驱动。此时，载片与水平面的倾斜角变小，以降低盖板与载片之间的试剂从缝隙底部流失的风险。第一驱动件320f停止驱动后，可以启动驱动机构400f工作，使盖板相对载片运动，以使盖板与载片间的标本和试剂混匀。当混匀结束后，驱动机构400f停机工作，第一驱动件320f的输出端收缩，以驱动孵育支架200f向下摆动，且摆动至托板310f与水平面平行时，第一驱动件320f停止驱动，此时孵育支架200f与水平面平行。
参考图53，在又一示例中，倾斜机构300f和孵育机构分别设置在基座100f上，驱动机构400f与倾斜机构300f连接。其中，基座100f可以包括底座110f和两个对称设置的支撑柱120f，支撑柱120f设置在基座100f的左端，且驱动机构400f设置在远离支撑柱120f的一侧，孵育支架200f的尾部朝向驱动机构400f。
倾斜机构300f可以包括第二驱动件321f和托板310f，托板310f的中部位置开设有第一连接孔和第二连接孔，位于内侧的支撑柱120f上设置有轴杆，轴杆能够穿过第一连接孔并在第一连接孔内转动。位于外侧的支撑柱120f上开设有一通孔，第二驱动件321f与该支撑柱120f连接，且第二驱动件321f的驱动轴穿过该通孔且与第二连接孔连接。第二驱动件321f可以是减速步进电机或舵机，第二驱动件321f可以驱动托板310f沿轴杆转动，以调整托板310f与水平面的倾斜度，其中，轴杆的轴线与第二驱动件321f的驱动轴的轴线相同。
基座100f上设置有支撑板130f，支撑板130f位于托板310f的侧边，支撑板130f上开设有弧形槽131f，托板310f上设有向外延伸的导杆313f，导杆313f能够在弧形槽131f内滑动，弧形槽131f用于限制托板310f上下摆动的距离。
驱动机构400f连接在托板310f的一端，驱动机构400f可以包括第三驱动件410f和连接块420f，第三驱动件410f可以为偏心轮电机，第三驱动件410f与连接块420f连接，连接块420f与盖板间接连接（具体连接方式请参考下文的孵育支架200f介绍）。当第三驱动件410f驱动连接块420f前后活动时，连接块420f能够带动盖板相对载片运动，以加快标本与试剂的反应速度，提供检测效率。
该孵育装置的工作过程可以是：初始状态时，托板310f与水平面保持平行，托板310f受两个支撑柱120f和支撑板130f的支撑。此时，可以向盖板与载片之间的缝隙添加液体。当初始状态要转换成孵育状态时，第二驱动件321f的输出端顺时针转动，托板310f沿轴杆向下摆动，对应的，孵育支架200f同步向下摆动。孵育支架200f向下摆动至孵育状态时，第二驱动件321f停止驱动。此时，载片与水平面的倾斜角变小，以降低盖板与载片之间的试剂从缝隙底部流失的风险。第二驱动件321f停止驱动后，可以启动驱动机构400f工作，使盖板相对载片运动，以使盖板与载片间的标本和试剂混匀。当混匀结束后，驱动机构400f停机工作，第二驱动件321f的输出端逆时针转动，以驱动孵育支架200f向上摆动，且摆动至托板310f与水平面平行时，第二驱动件321f停止驱动。
孵育支架200f初始状态时，孵育支架200f与水平面保持平行，底板240f的倾斜角的范围设置在15°至90°，对应的，载片230f的倾斜角的范围在15°至90°。例如，初始状态下，选择底板240f倾斜度为35°（与水平面的夹角）的孵育支架200f，对应的，载片230f的倾斜度为35°。初始状态下，选择底板240f倾斜角为75°的孵育支架200f，对应的，载片230f的倾斜角为75°，等等。
需要说明的是，在初始状态下，孵育支架200f水平放置在倾斜机构300f的托板310f上（初始状态下托板310f与水平面平行），而底板240f、载片230f和盖板220f与水平面均具有一定夹角。当倾斜机构300f驱动孵育支架200f摆动后，孵育支架200f能够由初始状态变成孵育状态，在孵育状态下，孵育支架200f与水平面呈一定夹角，而此时，孵育支架200f中的底板240f、载片230f和盖板220f与水平面的夹角变小。孵育状态下载片230f与水平面的倾斜角，小于初始状态下载片230f与水平面的倾斜角。
例如，可选择底板240f倾斜角为35°的孵育支架200f，初始状态时，载片230f相对于水平面的倾斜角为35°。通过倾斜机构300f驱动孵育支架200f摆动至孵育状态，此时，载片230f与水平面的倾斜角为0°（即载片230f与水平面平行）。还需要说明的是，孵育状态下载片230f与水平面的倾斜角可以为2°、3°、4°等等。
在本实施例中，通过孵育支架200f中的盖板220f和载片230f配合并倾斜设置于支架组件210f上，且倾斜机构300f与孵育支架200f的驱动连接，倾斜机构300f能够驱动孵育支架200f摆动，以调整盖板220f和载片230f相对水平面的倾斜度。这样在孵育过程中，能够使盖板220f与载片230f的倾斜度变小，避免倾斜角度过大而导致盖板220f与载片230f之间的试剂从缝隙底部流失，能够实现节约试剂用量，无需多次添加试剂，提高检测效率。
参考图54至图66，孵育支架200f可包括底板240f、载片230f、盖板220f、支架组件210f和推杆250f，底板240f用于装载载片230f，载片230f位于底板240f与盖板220f之间，盖板220f的两侧设置有凸起部221f。
支架组件210f包括一对侧板211f，一对侧板211f之间连接有底板240f，侧板211f与推杆250f滑动连接。推杆250f上开设有凹槽251f，凸起部221f设置于凹槽251f内。在外力的驱动下，推杆250f能够带动盖板220f运动，以搅动盖板220f与载片230f之间的试剂和组织样本。
一对推杆250f之间设置有连接板253f，连接板253f可以是具有磁性的金属，通过推动连接板253f能够使一对推杆250f同步的向第一方向Af运动。驱动机构400f上的连接块420f也具有磁性，连接板253f和连接块420f能够靠磁力吸在一起。连接块420f在第三驱动件410f的驱动下，带动连接板253f前后活动。
侧板211f开设有滑槽212f，推杆250f设置于滑槽212f内，推杆250f能够在滑槽212f内往复运动。在侧板211f上开设有限位槽213f以及与限位槽213f连通的导向槽216f，凹槽251f处于导向槽216f的下方，推杆250f在滑槽212f内往复滑动过程中，凹槽251f在某一位置上能够与导向槽216f连通。导向槽216f、限位槽213f和凹槽251f能够连通的结构，便于凸起部221f从限位槽213f、凹槽251f和导向槽216f取出或放入。使盖板220f的拆装操作简单，便于对盖板220f进行更换以及清洗，避免盖板220f长时间使用磨损、附着之前的试剂和组织样本，从而影响之后检测的准确性。限位槽213f、导向槽216f以及凹槽251f的宽度均大于圆柱体的直径，圆柱体能够在限位槽213f、导向槽216f和凹槽251f内自由活动。
参考图54、图55和图61，支架组件210f包括一对横板218f和一根横杆219f，一对横板218f和一根横杆219f参见实施例一至三中（例如，第一连接部107和第二连接部108等）的描述，在此不再赘述。
参考图54至图66，孵育支架200f包括多个底板240f以及多个盖板220f，底板240f与盖板220f一一对应。底板240f上设置有多个容置槽241f和多个凸起的限位块242f，一个容置槽241f的两侧设置有分别限位块242f，每个容置槽241f装置一片载片230f。限位块242f用于隔离相邻两个载片230f，以及限制载片230f沿垂直于底板240f方向上的移动。限位块242f可以是长方形、正方形等，在限位块242f的顶部可以设置有斜面（图未标识），斜面能够使载片230f导向插入容置槽241f内。
多个盖板220f分别设置于多个底板240f的上方，每个底板240f上可以插入多个载片230f。盖板220f与载片230f之间始终有部分位置贴合，试剂滴加在载片230f与盖板220f之间。
参考图55至图60，在一实施例中，盖板220f为弧形板222f。推杆250f在外力驱动下向第一方向Af滑动，迫使凸起部221f沿限位槽213f的第一端214f向第二端215f运动，弧形板222f的上部与载片230f逐渐分开，而盖板220f的下部就会以跷跷板的方式做相反的贴合运动，盖板220f始终有部分位置与载片230f贴合。推杆250f在外力驱动下向第一方向Af的相反方向滑动，迫使凸起部221f沿限位槽213f的第二端215f向第一端214f运动，盖板220f的上部与载片230f逐渐贴合，而盖板220f的下部就会以跷跷板的方式做相反的分开运动，盖板220f始终有部分位置与载片230f贴合。
采用弧形板222f更有利于促进载片230f上试剂大幅度的往复运动，强化动态孵育的效果，缩短孵育时间短，检测灵敏度较高。并且通过控制推拉组件往复运动的距离，可以控制弧形板222f与载片230f贴合的范围，缩小载片230f上试剂覆盖的区域，减少试剂的使用量，尤其是在进行如荧光原位杂交或使用其它昂贵试剂时，能节省成本。
其中，凹槽251f可以类似倒R字形，在凹槽251f的槽开口处向下延伸有倾斜面252f，倾斜面252f能够与导向槽216f的导向面217f衔接，这样便于凸起部221f沿着导向面217f以及倾斜面252f进出凹槽251f和限位槽213f。
参考图61至图66，在一实施例中，盖板220f为直板223f。推杆250f在外力驱动下向第一方向Af滑动，迫使凸起部221f沿限位槽213f的第一端214f向第二端215f运动，进而增大盖板220f与载片230f之间的开口。推杆250f在外力驱动下向第一方向Af的相反方向滑动，迫使凸起部221f沿限位槽213f的第二端215f向第一端214f运动，进而减小盖板220f与载片230f之间的开口。其中，凹槽251f可以类似倒直角梯形，在凹槽251f的槽开口处向下延伸有倾斜面252f。
参考图62~66，盖板220f上设置有多个毛细平面226f和凸条225f，凸条225f设在毛细平面226f的两侧边上，凸条225f由毛细平面226f的顶部延伸至底部。通过凸条225f抵压在载片230f上，盖板220f与载片230f间形成有缝隙。相邻两个毛细平面226f之间开设有隔离部224f，隔离部224f可以防止相邻两个载片230f上的试剂越界流动，避免试剂交叉污染，提高检测的准确性。
需要说明的是，在其他实施例中，孵育支架200f也可采用实施例一至四的孵育支架1、1a、1b和1c中的结构。
以上仅为本公开实施例的优选实施例而已，并不用于限制本公开实施例，对于本领域的技术人员来说，本公开实施例可以有各种更改和变化。凡在本公开实施例的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本公开实施例的保护范围之内。

Claims (15)

  1. 一种孵育支架，能够装载载片，其特征在于，包括支架组件以及倾斜设置于所述支架组件上的底板和盖板，所述底板用于装载所述载片，所述载片位于所述底板和所述盖板之间；所述盖板贴合在所述载片上滑动，或者，所述盖板相对所述载片开合运动，以混匀所述盖板与所述载片之间的试剂。
  2. 根据权利要求1所述的孵育支架，其特征在于，孵育支架还包括一对推动件，所述支架组件包括一对侧板，一对所述推动件分别滑动连接于一对所述侧板，所述底板和所述盖板均连接于一对所述侧板之间；
    所述盖板的上端设有凸起部，所述凸起部分别与一对所述推动件可分离式连接；
    所述侧板上开设有限位槽，所述限位槽设置有第一支撑面；
    所述推动件上开设有活动槽，所述活动槽设置有第二支撑面，所述第一支撑面和所述第二支撑面相互交叉设置，且所述凸起部能够支撑在所述第一支撑面和所述第二支撑面的交叉处；
    所述推动件能够在外力驱动下向第一方向滑动，迫使所述凸起部沿着所述第一支撑面和所述第二支撑面的交叉处向上活动，以增大所述盖板和所述载片之间的开口；所述推动件能够在外力驱动下向第二方向滑动，使所述凸起部能够沿着所述第一支撑面和所述第二支撑面的交叉处向下活动，以减小所述盖板和所述载片之间的开口。
  3. 根据权利要求1所述的孵育支架，其特征在于，还包括一对推动件，所述支架组件包括一对侧板，一对所述推动件分别滑动连接于一对所述侧板，所述底板和所述盖板均连接于一对所述侧板之间，所述侧板上开设有限位槽；
    所述盖板的上端设有凸起部，所述凸起部分别与一对所述推动件铰接；
    其中，所述推动件能够在外力驱动下沿第一方向往复滑动，迫使所述凸起部沿所述限位槽的第一端向第二端往复运动，以增大或减小所述盖板与所述载片之间的开口。
  4. 根据权利要求1所述的孵育支架，其特征在于，还包括一对推动件，所述支架组件包括一对侧板，一对所述推动件分别滑动连接于一对所述侧板，所述底板和所述盖板均连接于一对所述侧板之间；
    所述侧板上开设有第一限位槽，所述第一限位槽中具有两个平行设置的内侧壁；
    所述盖板的上端设有凸起部，所述凸起部分别与一对所述推动件铰接，所述盖板与所述载片贴合设置；
    其中，所述推动件能够在外力驱动下向第一方向往复滑动，迫使所述凸起部在所述内侧壁上往复运动，所述盖板贴合所述载片运动且所述盖板沿所述载片的长度方向做往复滑动。
  5. 根据权利要求1所述的孵育支架，其特征在于，所述支架组件包括上层支架和下层支架；
    所述底板上部的两侧与所述上层支架连接，所述底板下部的两侧与所述下层支架连接；
    所述盖板对应设置于所述底板一侧，且所述盖板上部的两侧与所述上层支架连接，所述盖板的下部能够贴靠于所述载片的表面；
    其中，所述上层支架在外力的驱动下能够相对于所述下层支架发生移动，使得所述上层支架与所述下层支架之间逐渐相对分离或靠拢，以增大或减小所述盖板与所述载片之间开口。
  6. 根据权利要求2~5中任意一项所述的孵育支架，其特征在于，所述孵育支架上至少设有两个所述底板，每个所述底板至少装载一个所述载片，每个所述底板对应设置有一个所述盖板，所述底板沿所述第一方向呈阵列排布。
  7. 根据权利要求2~5中任意一项所述的孵育支架，其特征在于，所述底板设置有第一隔离部，所述第一隔离部用于隔离和限位相邻两个所述载片；所述盖板设置有第二隔离部，所述第二隔离部用于隔离相邻两个所述载片上的试剂。
  8. 根据权利要求2~5中任意一项所述的孵育支架，其特征在于，所述盖板上设置有凸起的支撑台，所述支撑台的表面和所述载片贴合时，所述盖板和所述载片之间形成有毛细缝隙。
  9. 一种孵育装置，其特征在于，包括：
    根据权利要求1~8中任意一项所述的孵育支架；
    基座；
    倾斜机构，设置于所述基座上，与所述动支架组件驱动连接，用于驱动所述支架组件摆动，以调整所述底板、所述盖板和所述载片相对于水平面的倾斜角。
  10. 一种孵育箱，其特征在于，包括：
    壳体机构，设有安装腔；
    驱动机构，包括驱动件和从动件，所述驱动件与所述从动件驱动连接；
    孵育机构，包括支撑组件和如权利要求1~8中任意一项所述的孵育支架，所述支撑组件与所述安装腔的内壁连接；所述支撑组件包括设置有孵育槽的孵育锅，所述孵育支架设置于所述孵育槽；
    其中，所述盖板与所述传动件传动连接；所述驱动件能够驱动所述从动件运动，以带动所述盖板相对于所述载片运动。
  11. 根据权利要求10所述的孵育箱，其特征在于，所述支撑组件与所述安装腔的内壁以可推拉的方式活动连接。
  12. 根据权利要求10所述的孵育箱，其特征在于，所述支撑组件包括至少两个所述孵育锅，且不同所述孵育锅并排设置，每个所述孵育锅至少能够装载一个所述孵育支架。
  13. 一种染色设备，其特征在于，包括：
    机台；
    如权利要求10~12中任意一项所述的孵育箱；
    倾斜机构，设置于所述机台上，与所述孵育箱驱动连接，能够驱动所述孵育箱向下摆动，以使所述支架组件、所述盖板、所述底板和所述载片相对于水平面的倾斜度变小。
  14. 根据权利要求13所述的染色设备，其特征在于，所述设备还包括机械臂、加样针和试剂瓶，所述机械臂和所述试剂瓶设置于所述机台上，所述机械臂与所述加样针连接；所述机械臂能够带动所述加样针移动，且所述加样针能够抽取所述试剂瓶内试剂并滴入所述盖板与所述载片之间的缝隙。
  15. 一种染色方法，其特征在于，应用于如权利要求13~14中任一项所述的染色设备，所述方法包括：
    向盖板与载片之间的缝隙添加液体；
    倾斜机构驱动孵育箱向下摆动至孵育状态，所述倾斜机构停止驱动；
    所述盖板相对所述载片运动，以使所述盖板与所述载片间的液体混匀；
    所述倾斜机构驱动所述孵育箱向上摆动，且摆动至所述孵育箱与水平面平行时，所述倾斜机构停止驱动。
PCT/CN2024/087638 2023-04-13 2024-04-12 一种孵育支架、孵育装置、孵育箱、染色设备及染色方法 WO2024213150A1 (zh)

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