[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

WO2024211278A1 - Compositions de micro-organisme formant des spores avec des substances humiques et procédés d'utilisation associés - Google Patents

Compositions de micro-organisme formant des spores avec des substances humiques et procédés d'utilisation associés Download PDF

Info

Publication number
WO2024211278A1
WO2024211278A1 PCT/US2024/022622 US2024022622W WO2024211278A1 WO 2024211278 A1 WO2024211278 A1 WO 2024211278A1 US 2024022622 W US2024022622 W US 2024022622W WO 2024211278 A1 WO2024211278 A1 WO 2024211278A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
fertilizer
bacterial additive
bacterial
humic
Prior art date
Application number
PCT/US2024/022622
Other languages
English (en)
Inventor
Munif Hamad Nasser AL-MUNIF
Radwan M. ABDALLAH
James Edward HAIGH, Jr.
William Howard Diederich
Original Assignee
BiOWiSH Technologies, Inc.
Sabic Agri-Nutrients Company Sjsc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BiOWiSH Technologies, Inc., Sabic Agri-Nutrients Company Sjsc filed Critical BiOWiSH Technologies, Inc.
Publication of WO2024211278A1 publication Critical patent/WO2024211278A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C05D9/02—Other inorganic fertilisers containing trace elements
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/30—Layered or coated, e.g. dust-preventing coatings

Definitions

  • the present invention relates generally to compositions comprising spore forming microorganisms with humic substances, and use thereof.
  • the present invention also relates to methods of using a composition comprising spore forming microorganisms with humic substances for specialty usages such as increased nutrient use efficiency and/or crop nutrient availability.
  • the present invention also relates to applications in which both the microorganisms and the humic substances are delivered to the end use application via delivery vehicles including, but not limited to, mineral fertilizer carriers and/or seeds.
  • One method that can considered to improve plant growth includes the use of biostimulants and biofertilizers. Due to their resilience in a variety of physical and chemical stressors, endospores are a popular inclusion in microbial biostimulant and biofertilizer preparations. Many microbial-based agricultural formulations are delivered in either liquid or powder form. However, in practice, the commercial application of spore forming microbial products poses unique challenges in achieving long-term shelf life under a range of storage and use conditions.
  • compositions and methods for increasing the survivability of spore forming microbials and improving the biological efficiency of spore forming microbial based compositions comprising at least one bacterial additive and at least one humic substance, which supports stability and viability of the bacterial additive while improving biological efficiency of the product.
  • Microorganisms and humic substances in each case may be coupled with delivery vehicles including, but not limited to, mineral fertilizer carriers and/or seeds.
  • the present disclosure also provides compositions comprising at least one bacterial additive and at least one humic substance coated onto a mineral fertilizer carrier which will maintain or improve bacterial viability and/or adherence to the fertilizer carrier under environmental conditions.
  • compositions comprising at least one bacterial additive and at least one humic substance.
  • present disclosure provides compositions comprising at least one spore forming bacterial additive and at least one humic substance.
  • the compositions of the present disclosure provide microbial-based biostimulants or biofertilizer products that support stability and viability of the bacterial additive while improving biological efficiency of the product, and that can originate specialty usages such as increased nutrient efficiency and/or crop nutrient availability.
  • composition comprising at least one bacterial additive and at least one humic substance.
  • the at least one bacterial additive comprises endospore forming Bacillota.
  • the at least one bacterial additive comprises Bacillus spp, Brevibacillus spp, Paenibacillus spp, or Lysinibacillus spp.
  • the at least one bacterial additive comprises a Bacillus species.
  • the at least one bacterial additive comprises B. amyloliquefaciens (BA), B. subtilis (BS), B. pumilus (BP), B. licheniformis (BL), B. megaterium (BM) and B. thuringiensis (BT), or any combination thereof.
  • the at least one bacterial additive comprises a combination of B. amyloliquefaciens (BA), B. subtilis (BS), B. pumilus (BP), and B. licheniformis (BL). In some embodiments, the at least one bacterial additive comprises a combination of B. amyloliquefaciens (BA), B. pumilus (BP) and B. licheniformis (BL). In some embodiments, the at least one bacterial additive comprises endospores. In some embodiments, the endospores are suspended in a solution.
  • the at least one humic substance comprises humic acid, fulvic acid and/or humin. In some embodiments, the at least one humic substance comprises humic acid. In some embodiments, the at least one humic substance comprises fulvic acid. In some embodiments, the at least one humic substance comprises humin. In some embodiments, the at least one humic substance comprises seaweed extract.
  • the composition further comprises a mineral fertilizer.
  • the mineral fertilizer comprises urea.
  • the mineral fertilizer comprises phosphorus (P) and potassium (K).
  • the composition is blended with a liquid fertilizer or coated on a dry fertilizer.
  • the liquid fertilizer or dry fertilizer comprises nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S), magnesium (Mg), iron (Fe), boron (B), chlorine (Cl), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), nickel (Ni), cobalt (Co) or any combination thereof.
  • the liquid fertilizer comprises urea, ammonium nitrate, polyphosphate, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, blended and compounded nitrogen, phosphorus, and potassium (“NPK”), or any combination thereof.
  • the liquid fertilizer comprises a flowable suspension for fertigation, or any fertilizer capable of being dissolved.
  • the composition is blended with a liquid fertilizer or coated on a dry fertilizer.
  • the dry fertilizer comprises urea, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, blended and compounded NPK, or any combination thereof.
  • the composition is coated onto the seeds of a plant.
  • the composition comprises the at least one bacterial additive and the at least one humic substance present at a ratio in the range of 1 : 10 to 10: 1. In some embodiments, the at least one bacterial additive and the at least one humic substance are present at a ratio of 1 : 5.
  • the at least one bacterial additive comprises Bacillus spp, Brevibacillus spp, Paenibacillus spp, Lysinibacillus spp, or a combination thereof.
  • agronomic efficiency and/or partial factor productivity of the composition are increased relative to an otherwise equivalent composition comprising the at least one bacterial additive without the humic substance.
  • the composition improves crop nutrient assimilation, optimizes yield potential, enhances root development, improves plant vigor, enhances native microbial activity in the soil, improves soil productivity and/or improves soil health compared to plants that have not been treated with the composition, or plants that have been treated with an otherwise equivalent composition comprising the at least one bacterial additive without the humic substance.
  • the composition increases survivability of the bacterial additive.
  • the composition increases: (a) calcium stability of calcium contained on the coated dry fertilizer; (b) magnesium stability of magnesium contained on the coated dry fertilizer; (c) manganese stability of manganese contained on the coated dry fertilizer; (d) nitrogen stability of nitrogen contained on the coated dry fertilizer; (e) phosphorus stability of the phosphorus contained on the coated dry fertilizer; (f) zinc crop uptake when applied with zinc contained on the coated dry fertilizer; and/or (g) iron crop uptake when applied with iron contained on the coated dry fertilizer.
  • the composition improves calcium, magnesium, manganese, nitrogen, potassium, iron, and/or zinc plant uptake in most soils.
  • the composition increases survivability of a bacterial spore on a hostile surface.
  • the hostile surface comprises very high pH (basic), very low pH (acidic), very high level of chemicals that inhibit life, high levels of incomplete reactions that produced chemicals, urea, single super phosphate, and/or super triple phosphate.
  • the composition further comprises a surfactant and/or a wetter agent.
  • the composition humidity is below 20%.
  • the composition further comprises one or more micronutrients.
  • the composition is blended with a liquid fertilizer or coated onto a dry fertilizer.
  • a drying agent is applied after the composition is coated onto a dry fertilizer.
  • the composition further comprises an agent that drives modifications to a microbial phenotype.
  • the at least one bacterial additive comprises B. amyloliquefaciens, B. subtilis, B. pumilus, and B. licheniformis at a ratio of 1 : 1 : 1 : 1. In some embodiments, the at least one bacterial additive comprises B. amyloliquefaciens, B. pumilus, and B. licheniformis at a ratio of 1 : 1 : 1.
  • the disclosure provides a composition comprising: (a) a liquid component comprising the composition of the disclosure; and (b) a carrier component comprising a mineral fertilizer or seed.
  • the disclosure provides a method using the composition of the disclosure, the method comprising a specialty usage.
  • the specialty usage comprises increasing nutrient use efficiency and/or crop nutrient availability.
  • the nutrient comprises nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, non-essential nutrients, and/or micronutrients.
  • the non-essential nutrients comprise titanium, selenium, and/or silicon.
  • FIG. 1 shows the effect of treating corn with a composition of the present disclosure on the corn yield.
  • the corn yield was recorded by weighing the dried grains per plot.
  • the data of yield of corn grain was recorded as kg per plot.
  • compositions and methods for increasing nutrient use efficiency (NUE) and/or crop nutrient availability, and increasing the survivability of spore forming microbials and improving the biological efficiency of spore forming microbial based compositions comprise at least one bacterial additive and at least one humic substance.
  • compositions and methods for increasing the survivability of spore forming microbials and improving the biological efficiency of spore forming microbial based compositions comprising at least one bacterial additive and at least one humic substance.
  • compositions and methods described herein provide certain advantages over other compositions and methods including, but not limited to, increasing stability and viability of the bacterial additive, improving biological efficiency of the microbial product, and promoting desirable microbial phenotypes which may adventitiously result in specialty usages such as increased nutrient efficiency and/or increased crop nutrient availability.
  • compositions comprising at least one bacterial additive and at least one humic substance.
  • the at least one bacterial additive comprises at least one spore forming bacterial additive.
  • the compositions of the present disclosure can also provide microbial-based biostimulants or biofertilizer products that support stability and viability of the bacterial additive while increasing biological efficiency of the product, nutrient efficiency and/or crop nutrient availability.
  • compositions comprising a) a liquid component comprising at least one bacterial additive and at least one humic substance; and b) a carrier component comprising a mineral fertilizer or a seed.
  • the compositions of the present disclosure will maintain or improve bacterial viability and/or adherence to the carrier under environmental conditions.
  • the at least one bacterial additive comprises endospore forming Bacillota.
  • the at least one bacterial additive comprises Bacillus spp, Brevibacillus spp, Paenibacillus spp, Lysinibacillus spp, and/or another Bacillota species, wherein spp refers to species within a genus.
  • Bacillus spp refers to one or more species within the genus, such as the genus Bacillus.
  • the at least one bacterial additive is Bacillus spp, Brevibacillus spp, Paenibacillus spp, and/or Lysinibacillus spp.
  • the Bacillus species is B. amyloliquefaciens (BA), B. subtilis (BS), B. pumilus (BP), B. licheniformis (BL), B. megaterium (BM) and B. thuringiensis (BT) or another Bacillus species.
  • the bacterial additive comprises one or more endospore forming Bacillota.
  • Bacillota are a phylum of bacteria, which includes the Bacillus genus.
  • the one or more endospore forming Bacillota is Bacillus spp, Brevibacillus spp, Paenibacillus spp, Lysinibacillus spp, another Bacillota species, or any combination thereof.
  • the one or more endospore forming Bacillota is a Bacillus, Brevibacillus, Paenibacillus, or Lysinibacillus species.
  • the Bacillus species is B. amyloliquefaciens (BA), B. subtilis (BS), B. pumilus (BP), B. licheniformis (BL), Priestia megaterium (basonym B. megaterium (BM)) B.
  • Bacillus species is B. amyloliquefaciens (BA), B. subtilis (BS), B. pumilus (BP), and/or B. licheniformis (BL).
  • Bacillus species is B. amyloliquefaciens (BA), B. pumilus (BP), and/or B. licheniformis (BL).
  • Bacillota species such as Bacillus, Brevibacillus, Paenibacillus, Lysinibacillus genus species are known to persons of ordinary skill in the art. Such species are available from generally recognized depositories. Exemplary deposit information for bacterial species that can be used in the in the compositions of the disclosure is provided in Table A below.
  • the at least one bacterial additive comprises a liquid endospore solution (i.e., in a solution).
  • the liquid endospore suspension comprises at least one endospore forming Bacillota.
  • the one or more endospore forming Bacillota is Bacillus spp, Brevibacillus spp, Paenibacillus spp, Lysinibacillus spp, another Bacillota species, or any combination thereof.
  • liquid endospore suspension has a microbial concentration from about 1X10 6 to about 1X10 12 colony forming units per mL (CFU/mL), e.g., 1X10 6 to about 1X10 11 CFU/mL, about 1X10 8 to 1X10 12 CFU/mL, or about 1X10 9 to 1X10 12 CFU/mL.
  • the liquid endospore has a microbial concentration comprising about 1X10 7 to about 1X10 11 CFU/mL.
  • the liquid endospore suspension has a microbial concentration comprising about 1X10 8 to about 1X10 10 CFU/mL.
  • the liquid endospore suspension has a microbial concentration comprising about 5X10 8 to about 1X10 10 CFU/mL. In some embodiments, the liquid endospore suspension has a microbial concentration comprising about 5X10 8 to about 5X10 9 CFU/mL. Exemplary liquid endospore solutions are described in PCT/US2023/079253, the contents of which are incorporated by reference in its entirety herein.
  • the liquid endospore suspension comprises one or more preservative agents, buffering agents, suspending agents, surfactants, or a combination thereof.
  • preservative agents buffering agents, suspending agents, surfactants, or a combination thereof.
  • buffering agents suspending agents
  • surfactants or a combination thereof.
  • additional preservative agents, buffering agents, suspending agents and surfactants are described in WO 2021/022128, the contents of which are incorporated by reference in their entirety herein.
  • the one or more preservative agents can be selected from a modified isothiazolin compound, an ester of p-hydroxybenzoic acid, a modified quaternary amine, a modified urea, a glycerin derivative, 2-bromo-2-nitro-l,3-propanediol, a natural oil, an organic acid having a molecular weight of no more than 200 and at least one pKa greater than 4.2, or any combination thereof.
  • the one or more preservative agents comprises calcium sulfate.
  • the liquid endospore suspension can include about 0.01 wt% to 15.0 wt% of the one or more preservative agents, e.g., about 0.01 wt% to 10.0 wt%, about 0.01 wt% to 5.0 wt%, about 0.01 wt% to 3.0 wt%, about 0.01 wt% to 1.0 wt%, about 0.1 wt% to 15.0 wt%, about 0.1 wt% to 10.0 wt%, about 0.1 wt% to 5.0 wt%, about 0.1 wt% to 3.0 wt%, about 0.1 wt% to 1.0 wt%, about 0.5 wt% to 15.0 wt%, about 0.5 wt% to 10.0 wt%, about 0.5 wt% to 5.0 wt%, about 0.5 wt% to 3.0 wt%, about 0.5 wt% to 1.0 wt%, about 0.5 w
  • the liquid endospore solution can include one suspending agent, two suspending agents, three suspending agents, or more than three suspending agents.
  • the suspending agent is used, inter alia, for suspending the microbes in the aqueous composition, thereby providing the desirable physical stability.
  • the suspending agent is a polymer, a surfactant, or a combination thereof.
  • the suspending agent can include xanthan gum, acacia gum, alcohol ethoxylate, or a combination thereof.
  • the suspension agent comprises as rheological modifier.
  • Rheological modifiers are substances that alter the rheological properties of a material, i.e. that are added to a formulation to alter viscosity.
  • Suitable rheological modifiers can include, but are not limited to, commercially available rheological modifiers comprising urea and modified versions of urea, such as RHEOBYK®-410.
  • the liquid endospore suspension can include about 0.01 wt% to 15.0 wt% buffering agent, e.g., about 0.01 wt% to 10.0 wt%, about 0.01 wt% to 5.0 wt%, about 0.01 wt% to 3.0 wt%, about 0.01 wt% to 1.0 wt%, about 0.1 wt% to 15.0 wt%, about 0.1 wt% to 10.0 wt%, about 0.1 wt% to 5.0 wt%, about 0.1 wt% to 3.0 wt%, about 0.1 wt% to 1.0 wt%, about 0.5 wt% to 15.0 wt%, about 0.5 wt% to 10.0 wt%, about 0.5 wt% to 5.0 wt%, about 0.5 wt% to 3.0 wt%, about 0.5 wt% to 1.0 wt%, about 1.0
  • the buffering agent can be in an amount sufficient to keep the pH of the liquid endospore suspension at greater than 4.2, e.g., greater than 4.5, greater than 5.0, greater than 5.5, greater than 6.0, greater than 6.5, or greater than 7.0.
  • the buffering agent can be in an amount sufficient to keep the pH of the composition in the range of about 4.3 to about 12.0, e.g., about 4.3 to about 11.0, about 4.3 to about 10.0, about 4.3 to about 9.5, about 4.3 to about 9.0, about 4.3 to about 8.5, about 5.0 to about 12.0, about 5.0 to about 11.0, about 5.0 to about 10.0, about 5.0 to about 9.5, about 5.0 to about 9.0, about 5.0 to about 8.5, about 5.5 to about 12.0, about 5.5 to about 11.0, about 5.5 to about 10.0, about 5.5 to about 9.5, about 5.5 to about 9.0, or about 5.5 to about 8.5.
  • the pH of a liquid composition or liquid endospore suspension of the present disclosure can be selected to maximize shelf life of the spores and/or colonies stored in the composition for extended periods. pH’s less than about 4 may have a detrimental effect on stability of certain bacterial spores at elevated temperatures. For example, Bacillus spores can demineralize at low pH, losing their resistance to high temperature storage. Therefore, a pH greater than about 4.2 is desirable for improving the biological stability of Bacillus spores.
  • the composition including at least one bacterial additive and at least one humic substance increases survivability of a bacterial spore on a hostile surface.
  • the hostile surface comprises very high pH (basic), very low pH (acidic), very high level of chemicals that inhibit life, high levels of incomplete reactions that produced chemicals, urea, single super phosphate, and/or super triple phosphate.
  • compositions comprising at least one humic substance.
  • the at least one humic substance comprises at least one humic acid, fulvic acid, humin, or any combination thereof.
  • the at least one humic substance comprises at least one humic acid, fulvic acid and/or humin.
  • the humic substance is a humic acid, a fulvic acid or a humin.
  • the humic substance can include at least one humic substance, at least two humic substances, at least three humic substances, or four or more humic substances.
  • the one or more humic substances comprises seaweed extract.
  • the one or more humic substances is extracted from seaweed extract.
  • the humic substance originated from industrial processes.
  • the humic substance is extracted from Leonardite.
  • the humic substance is extracted using redox reactions, dissolution, high pressure and temperature extractions, and/or chemical dissolution with strong acids and bases.
  • the humic substance may include long and/or short chain humic derived molecules.
  • the at least one bacterial additive and at least one humic substance are present at a ratio in the range of about 10:1 to 1: 10 in the composition.
  • the bacterial additive comprises Bacillus spp, Brevibacillus spp, Paenibacillus spp, and/or Lysinibacillus spp.
  • the at least one bacterial additive and the at least one humic substance are present at a ratio in the range of about 20: 1 to 1 :20.
  • the at least one bacterial additive and the at least one humic substance are present at a ratio in the range of about 15: 1 to 1 : 15.
  • at least the one bacterial additive and the at least one humic substance are present at a ratio in the range of about 10: 1 to 1 : 10.
  • the at least one bacterial additive and the at least one humic substance are present at a ratio in the range of about 10: 1, 9:1, 8: 1, 7:1, 6: 1, 5:1, 4: 1, 3:1, 2: 1, 1: 1, 1:2, 1:3, 1:4, 1:5, 1 :6, 1:7, 1 :8, 1:9, or 1 : 10.
  • the at least one bacterial additive and the at least one humic substance are present at a ratio of about 1 :5.
  • the at least one bacterial additive and the at least one humic substance are present at a ratio of about 1:1.
  • the composition comprising at least one bacterial additive and at least one humic substance further comprises at least one surfactant and/or wetter agent.
  • wetter agents include, but are not limited to, propylene glycol and glycerin.
  • surfactants include, but are not limited to, a nonionic surfactant, a primary alkyl alcohol ethoxylate, a secondary alkyl alcohol ethoxylate, a primary alkyl alcohol propoxylate, a secondary alkyl alcohol propoxy late, and/or any combination thereof.
  • the surfactant has a cloud point from about 30 °C to about 80 °C, and hydrophilic-lipophilic balance from about 5 to about 15.
  • the surfactant is a C- 13 branched primary alcohol with average ethoxylation of 5 to lO, e.g., 5, 6, 7, 8, 9, or 10.
  • the surfactant includes an ethoxylated primary branched Cl 3 alcohol with full saturation, such as SynperonicTM 13/7 or SynperonicTM 13/6.
  • the surfactant includes alcohol ethoxylate, such as EcosurfTM EH-6.
  • the surfactant includes a mixture of 58.0-62.0% D-glucopyranose, oligomeric, decyl octyl glycoside and 38.0-42.0% water, and contains less than 2% decanol and less than 1.0% octanol, such as TritonTM CGI 10.
  • the surfactant includes secondary polyether polyol, such as TergitolTM L-62.
  • the surfactant includes secondary alcohol ethoxylate, such as TergitolTM 15-S-12.
  • the surfactant includes a non-ionic alkyl EO/PO copolymer, such as TergitolTM XDLW.
  • the composition comprising at least one bacterial additive and at least one humic substance can include about 0.1 wt% to 10 wt% surfactant, e.g., about 0.1 wt% to 5 wt%, 0.1 wt% to 2 wt%, 0.1 wt% to 1 wt%, 0.5 wt% to 1 wt%, or 0.5 wt% to 2 wt% surfactant.
  • compositions of the present disclosure can provide a number of desirable characteristics related to decreased spoilage, endospore stability, and/or specialty usages including, but not limited to, increasing nutrient use efficiency and/or crop nutrient availability.
  • the disclosure provides fertilizer compositions comprising microbial-based biostimulants or biofertilizer products that support stability and viability of the bacterial additive while increasing biological efficiency of the product, nutrient use efficiency and/or crop nutrient availability.
  • the composition comprises a) a liquid component comprising at least one bacterial additive and at least one humic substance; and b) a carrier component.
  • the carrier component comprises a mineral fertilizer or a seed.
  • the compositions of the present disclosure will maintain or improve bacterial viability and/or adherence to the carrier under environmental conditions.
  • the composition comprising at least one bacterial additive and at least one humic substance is blended with a liquid fertilizer or coated on a dry fertilizer.
  • the present disclosure relates to fertilizing compositions comprising a liquid fertilizer in combination with a composition comprising at least one bacterial additive and at least one humic substance described herein. In other embodiments, the present disclosure relates to fertilizing compositions comprising a dry fertilizer coated with a composition comprising at least one bacterial additive and at least one humic substance described herein.
  • the fertilizer is a mineral fertilizer. In some embodiments, the mineral fertilizer comprises urea, monoammonium phosphate, diammonium phosphate, and/or various blended and compounded NPKs (nitrogen, e.g., urea, combined with phosphate and potassium sources).
  • the fertilizer is an organic fertilizer.
  • the organic fertilizer comprises fish meal, bird guano, livestock manure, compost, and/or rock phosphate.
  • Another aspect of the present disclosure relates to a fertilizing composition coated onto seeds.
  • the fertilizing composition comprises a composition comprising at least one bacterial additive and at least one humic substance described herein.
  • a composition comprising at least one bacterial additive and at least one humic substance is coated onto seeds of any plant.
  • the compositions described herein can be coated onto seeds of any plant including, but not limited to, rice, corn, soybean, onion, sugar cane, tomato, barley, lettuce, wheat, potato, legumes, or grass.
  • a composition of the present disclosure can be used as a biostimulant and/or a component of enhanced an efficiency fertilizer in agronomy applications to promote plant health and vigor and improve crop yield.
  • it can be applied to soil after being coated onto carriers or via irrigation/chemigation processes, or via tank mixed with other agrochemicals, or coated onto seeds.
  • the composition comprising at least one bacterial additive and at least one humic substance may be coated onto a nutritive fertilizer.
  • the composition comprising at least one bacterial additive and at least one humic substance may be coated onto common fertilizer particles such as urea, monoammonium and diammonium phosphate, ammonium sulfate, compound and blended NPKs, and biosolids.
  • the resulting coated fertilizer particles can then be field applied via standard fertilization practices known in the art.
  • the composition comprising at least one bacterial additive and at least one humic substance may be mixed with liquid fertilizers and applied as part of a standard fertilization regimen known in the art.
  • the composition comprising at least one bacterial additive and at least one humic substance may be combined with other agrochemicals such as fungicides, herbicides, or insecticides in a tank mix and applied via spray application.
  • the composition comprising at least one bacterial additive and at least one humic substance may also be applied directly to the leaves of a plant via foliar application.
  • compositions of the present invention comprising at least one bacterial additive and at least one humic substance are added to liquid fertilizers certified for use in organic farming (e.g. PhytaminTM, Phyta-QCTM, Tridents Pride, etc.) and the subsequent blended liquid is used as part of an organic crop fertility program.
  • the liquid fertilizer comprises a flowable suspension for fertigation in combination with urea, ammonium nitrate, polyphosphate, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, NPK (blended and compounded), and/or any fertilizer capable of being dissolved.
  • compositions of the present invention comprising at least one bacterial additive and at least one humic substance are coated onto a dry fertilizer.
  • the dry fertilizer is an organic fertilizer.
  • the organic fertilizer includes fish meal, bird guano, livestock manure, compost, and/or rock phosphate.
  • the dry fertilizer is a mineral fertilizer.
  • the mineral fertilizer comprises urea, monoammonium phosphate, diammonium phosphate, and/or various blended and compounded Nitrogen, Phosphorous, and Potassium (“NPK”).
  • the dry fertilizer comprises urea, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, and/or NPK (blended and compounded).
  • a drying agent is applied (postcoating).
  • the drying agent is ammonium sulfate, diammonium phosphate, ammonium phosphate, ammonium nitrate, another ammonia-based drying agent, or any combination thereof.
  • the drying agent is applied to granular urea following coating with a composition comprising at least one bacterial additive and at least one humic substance.
  • the liquid fertilizer or dry fertilizer comprises nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S), magnesium (Mg), iron (Fe), boron (B), chlorine (Cl), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), nickel (Ni), cobalt (Co) or any combination thereof.
  • a surfactant and/or a wetter agent may be added to the composition.
  • the addition of an appropriate surfactant and/or wetter agent may also aid steric stabilization by preventing the spores from clumping together.
  • the wetter agents comprises propylene glycol and glycerin.
  • the surfactant comprises a nonionic surfactant, a primary alkyl alcohol ethoxylate, a secondary alkyl alcohol ethoxylate, a primary alkyl alcohol propoxylate, a secondary alkyl alcohol propoxylate, and/or any combination thereof.
  • the disclosure provides methods for fertilizing crops using any of the compositions described herein.
  • the method comprises contacting the crop with a composition comprising at least one bacterial additive and at least one humic substance.
  • the crop is an organic crop.
  • the crop is not an organic crop.
  • the crop is grown using conventional farming practices (i.e., the management of crop resource input uniformly including the use of chemical fertilizers and pesticides). Examples of crops include, but are not limited to, rice, corn, soybean, onion, sugar cane, tomato, potato, barley, wheat, legume, lettuce, cotton, oat, and grass.
  • the method comprises contacting the crop with the composition comprising at least one bacterial additive and at least one humic substance, at least once during growth season, e.g., 1-3 times.
  • the contacting of the crop comprises applying the composition comprising at least one bacterial additive and at least one humic substance to soil, an irrigation system, and/or the leaves of the crop.
  • the method further comprises mixing the composition comprising at least one bacterial additive and at least one humic substance with a fertilizer prior to contacting the crop.
  • compositions of the present disclosure can provide a number of desirable characteristics related to soil health and improvement of soil quality, such as increased nutrient availability, increased organic matter content, decreased compaction, and improved moisture retention.
  • the composition comprising at least one bacterial additive and at least one humic substance can also provide a number of desirable characteristics related to plant health and vigor including, but not limited to, improved nutrient use uptake, improved abiotic stress tolerance, increased plant biomass, increased vegetation index (ND VI), and increased crop yield.
  • the composition including at least one bacterial additive and at least one humic substance can improve crop nutrient assimilation, can optimize yield potential, can enhance root development, can improve plant vigor, can enhance native microbial activity in the soil, can improve soil productivity and/or can improve soil health.
  • the composition can improve crop assimilation of essential and/or non-essential nutrients.
  • the composition improves crop assimilation of essential nutrients.
  • the composition including at least one bacterial additive and at least one humic substance improves crop assimilation of calcium, magnesium, manganese, nitrogen, phosphorus, zinc, iron, another essential nutrient, or any combination thereof.
  • the composition including at least one bacterial additive and at least one humic substance improves crop assimilation of calcium, magnesium, manganese, nitrogen, potassium, phosphorus, zinc, and/or iron. In some embodiments, the composition improves calcium, magnesium, manganese, nitrogen, potassium, iron, and/or zinc plant uptake in most soils.
  • the composition maintains or increases: a) calcium stability of calcium contained on the coated dry fertilizer; b) magnesium stability of magnesium contained on the coated dry fertilizer; c) manganese stability of manganese contained on the coated dry fertilizer; d) nitrogen stability of nitrogen contained on the coated dry fertilizer; e) phosphorus stability of the phosphorus contained on the coated dry fertilizer; f) zinc crop uptake when applied with zinc contained on the coated dry fertilizer; and/or g) iron crop uptake when applied with iron contained on the coated dry fertilizer.
  • the composition including at least one bacterial additive and at least one humic substance can improve crop assimilation of non-essential nutrients.
  • the composition including at least one bacterial additive and at least one humic substance improves crop assimilation of titanium, selenium, and/or silicon, another non-essential nutrient, or any combination thereof.
  • the composition including at least one bacterial additive and at least one humic substance improves crop assimilation of titanium, selenium, and/or silicon.
  • the composition improves titanium, selenium, and/or silicon plant uptake in most soils.
  • the method increases nutrient use efficiency and/or crop nutrient availability of non-essential nutrients.
  • the method increases nutrient use efficiency and/or crop nutrient availability of titanium, selenium, and/or silicon, another non-essential nutrient, or any combination thereof.
  • the method increases nutrient use efficiency and/or crop nutrient availability of titanium, selenium, and/or silicon.
  • the method increases nutrient use efficiency and/or crop nutrient availability of titanium, selenium, and/or silicon for plant uptake in most soils.
  • the method increases nutrient use efficiency and/or crop nutrient availability wherein the nutrient comprises essential nutrients, non-essential nutrients, and/or micronutrients. In some embodiments, the method increases nutrient use efficiency and/or crop nutrient availability wherein the nutrient comprises nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, non-essential nutrients, and/or micronutrients.
  • the method increases nutrient use efficiency and/or crop nutrient availability of essential nutrients.
  • the method increases nutrient use efficiency and/or crop nutrient availability of calcium, magnesium, manganese, nitrogen, potassium phosphorus, zinc, iron, another essential nutrient, or any combination thereof.
  • the method increases nutrient use efficiency and/or crop nutrient availability of calcium, magnesium, manganese, nitrogen, potassium, phosphorus, zinc, and/or iron.
  • the method increases nutrient use efficiency and/or crop nutrient availability of calcium, magnesium, manganese, nitrogen, potassium, iron, and/or zinc for plant uptake in most soils.
  • Another aspect of the present disclosure relates to the method of using the composition comprising at least one bacterial additive and at least one humic substance, wherein the method comprises a specialty usage.
  • the specialty usage comprises increased nutrient use efficiency and/or crop nutrient availability.
  • the method of using the composition comprising at least one bacterial additive and at least one humic substance increases nutrient use efficiency.
  • the method of using the composition comprising at least one bacterial additive and at least one humic substance increases crop nutrient availability.
  • compositions of the present disclosure can be antagonistic to spoilage organisms while simultaneously supportive of endospore dormancy and stability.
  • the disclosure also provides methods of inhibiting the proliferation of at least one environmental spoilage organism in a liquid endospore suspension, wherein the method comprises use of at least one osmotic preservative.
  • the at least one environmental spoilage organism includes, but is not limited to a bacterial, a yeast, or a fungus contaminant.
  • the composition including at least one bacterial additive and at least one humic substance can maintain or improve biological stability at about 20 °C to about 40 °C for at least 3 months, at least 6 months, at least 7 months, at least 8 months, at least 9 months, at least 10 months, at least 11 months, at least a year, at least 1.5 years, or at least 2 years.
  • the composition including at least one bacterial additive and at least one humic substance can maintain or improve biological stability at about 20 °C to about 40 °C for about 6 months to about 3 years, e.g., about 6 months to about 2 years, about 6 months to about 1.5 years, about 6 months to about 1 year, about 1 year to about 3 years, about 1 year to 2 years, or about 1 year to 1.5 years.
  • the composition including at least one bacterial additive and at least one humic substance can maintain or improve biological stability at about 20 °C to about 40 °C for about 3 months, about 6 months, about 9 months, about a year, about 1.5 years, or about 2 years.
  • the composition including at least one bacterial additive and at least one humic substance can maintain or improve biological stability at about 40 °C for about 6 months (i.e. 180 days).
  • the composition including at least one bacterial additive and at least one humic substance can maintain or improve biological stability at room temperature for at least 3 months, at least 6 months, at least 7 months, at least 8 months, at least 9 months, at least 10 months, at least 11 months, at least a year, at least 1.5 years, or at least 2 years.
  • the composition including at least one bacterial additive and at least one humic substance can maintain or improve biological stability at room temperature for about 6 months to about 3 years, e.g., about 6 months to about 2 years, about 6 months to about 1.5 years, about 6 months to about 1 year, about 1 year to about 3 years, about 1 year to 2 years, or about 1 year to 1.5 years.
  • the composition including at least one bacterial additive and at least one humic substance can maintain or improve biological stability at room temperature for about 3 months, about 6 months, about 9 months, about a year, about 1.5 years, about 2 years, about 2.5 years, or about 3 years.
  • the composition including at least one bacterial additive and at least one humic substance can improve the biological performance of the bacterial additive at about 20 °C to about 40 °C for at least 3 months, at least 6 months, at least 7 months, at least 8 months, at least 9 months, at least 10 months, at least 11 months, at least a year, at least 1.5 years, or at least 2 years.
  • the composition including at least one bacterial additive and at least one humic substance can improve the biological performance of the bacterial additive at about 20 °C to about 40 °C for about 6 months to about 3 years, e.g., about 6 months to about 2 years, about 6 months to about 1.5 years, about 6 months to about 1 year, about 1 year to about 3 years, about 1 year to 2 years, or about 1 year to 1.5 years.
  • the composition including at least one bacterial additive and at least one humic substance can improve the biological performance of the bacterial additive at about 20 °C to about 40 °C for about 3 months, about 6 months, about 9 months, about a year, about 1.5 years, or about 2 years.
  • the composition including at least one bacterial additive and at least one humic substance can improve the biological performance of the bacterial additive at about 40 °C for about 6 months (i.e. 180 days).
  • the composition including at least one bacterial additive and at least one humic substance can improve the biological performance of the bacterial additive at room temperature for at least 3 months, at least 6 months, at least 7 months, at least 8 months, at least 9 months, at least 10 months, at least 11 months, at least a year, at least 1.5 years, or at least 2 years.
  • the composition including at least one bacterial additive and at least one humic substance can improve the biological performance of the bacterial additive at room temperature for about 6 months to about 3 years, e.g., about 6 months to about 2 years, about 6 months to about 1.5 years, about 6 months to about 1 year, about 1 year to about 3 years, about 1 year to 2 years, or about 1 year to 1.5 years.
  • the composition including at least one bacterial additive and at least one humic substance can improve the biological performance of the bacterial additive at room temperature for about 3 months, about 6 months, about 9 months, about a year, about 1.5 years, about 2 years, about 2.5 years, or about 3 years.
  • the composition including at least one bacterial additive and at least one humic substance can improve the biological performance of the bacterial additive. In some embodiments, the composition including at least one bacterial additive and at least one humic substance increases survivability of the bacterial additive. In some embodiments, the compositions of the present disclosure can increase survivability of the at least one bacterial additive by at least 5% as compared to a control composition where the at least one humic substance is not used.
  • the composition can increase survivability of the at least one bacterial additive by at least 6%, at least 7%, at least 8%, at least 9%, at least 10%, at least 20%, at least 30%, at least 40%, or at least 50% as compared to a control composition where the at least one humic substance is not used. In some embodiments, the composition can increase survivability of the at least one bacterial additive by about 10% to 200% as compared to a control composition where the at least one humic substance is not used. Survivability can be measured by plate count assays.
  • the composition including at least one bacterial additive and at least one humic substance can remain biologically and/or physically stable for the aforementioned periods of time under a variety of humidity conditions.
  • the humidity is ambient.
  • the humidity is about 10% to about 90%, e.g., about 10% to about 80%, about 10% to about 75%, about 10% to about 70%, about 10% to about 65%, about 10% to about 60%, about 20% to about 90%, about 20% to about 80%, about 20% to about 75%, about 20% to about 70%, about 20% to about 65%, about 20% to about 60%, about 30% to about 90%, about 30% to about 80%, about 30% to about 75%, about 30% to about 70%, about 30% to about 65%, or about 30% to about 60%.
  • the humidity is about 10%, about 15%, about 20%, about
  • the humidity is below about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about
  • the humidity is below 10%, 15%, 20%,
  • composition humidity is below 20%.
  • the composition maintains or increases: a) calcium stability of calcium contained on the coated dry fertilizer; b) magnesium stability of magnesium contained on the coated dry fertilizer; c) manganese stability of manganese contained on the coated dry fertilizer; d) nitrogen stability of nitrogen contained on the coated dry fertilizer; e) phosphorus stability of the phosphorus contained on the coated dry fertilizer; or any combination thereof.
  • inventive embodiments are presented by way of example only and that, within the scope of the appended claims and equivalents thereto, inventive embodiments may be practiced otherwise than as specifically described and claimed.
  • inventive embodiments of the present disclosure are directed to each individual feature, system, article, material, kit, and/or method described herein.
  • a hostile surface refers to a surface that decreases the survivability of a microorganism, including but not limited to bacterial spores.
  • a hostile surface may have an overly acidic pH or an overly basic pH.
  • Another example of a hostile surface may include, but is not limited to, an overly smooth particle surface and other retention-based issues.
  • a hostile surface should be understood to include, but not limited to, a surface that is hostile to microbial survivability and/or retention potential.
  • a non-limiting example of a hostile surface may include, but is not limited to, prilled fertilizers and other overly-smooth particles with few harborage sites for endospore adsorption.
  • liquid fertilizer refers to any form of liquid fertilizer available, any flowable suspension for fertigation and any fertilizer capable of being dissolved.
  • liquid fertilizer may include, but is not limited to, urea, ammonium nitrate, polyphosphate, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, and/or NPK (blended and compounded).
  • the liquid fertilizer may include nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S), magnesium (Mg), iron (Fe), boron (B), chlorine (Cl), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), nickel (Ni), cobalt (Co) or any combination thereof.
  • dry fertilizer refers to any form of dry fertilizer available, any fertilizer capable of being blended and/or compounded to produce a dry mixture.
  • dry fertilizer may include, but is not limited to, urea, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, and/or NPK (blended and compounded).
  • the dry fertilizer may include nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S), magnesium (Mg), iron (Fe), boron (B), chlorine (Cl), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), nickel (Ni), cobalt (Co) or any combination thereof.
  • the fertilizer can be understood to be granular (e.g. granular urea).
  • NPK Nitrogen Phosphorus Potassium fertilizers which include the macro-nutrients nitrogen (N), phosphorous (P), and potassium (k) in varying amounts. The amount of each macro-nutrient component may be varied dependent on plant nutrient needs. NPK fertilizers may be compound, wherein the components are chemically bound, or blended, wherein the single nutrient components in a physical mixture.
  • drying agent refers to one or more inorganic salts applied to a dry fertilizer.
  • the drying agent may include, but is not limited to, ammonium sulfate, diammonium phosphate, ammonium phosphate, ammonium nitrate, another ammonia-based drying agent, or any combination thereof. These compounds may be applied following application of a composition of the of the present disclosure to a fertilizer.
  • humic substance refers to one or more humic substances or at least one humic substance.
  • Humic substances refer to a category of compounds which are present in soil and other organic matter. These compounds are not readily decomposed and are known to mediate many chemical reactions and are thought to play a role in the reduction of soil toxicity.
  • Humic substances may be derived from decomposed plant and animal material including lignin, tannins, cellulose and cutins; or can be commercially or industrially produced from coal, lignite, Leonardite, or other organic materials.
  • Humic substances may also be derived from seaweed and/or seaweed extracts (e.g. kelp).
  • a humic substance can be understood to include, but is not limited to, humic substances extracted through industrial processes including, but not limited to, redox reactions, dissolution, high pressure and temperature extractions, and chemical dissolution with strong acids and bases.
  • a humic substance may include long and/or short chain humic derived molecules.
  • a humic substance may include, but is not limited, industrial humic substances and non-Industrial or Commercial humic substances.
  • Industrial humic substances are defined as a preparation of specific molecules from the humic molecular family, sold as either single molecules or blends of molecules, produced using specific industrial processes.
  • the separation of molecular groups is based on specific chemical processes (condensation, redox, complexation, and many others) that may re-group a super mixture of molecules using industrialized chemical set ups. These molecules, or groups of molecules, may be selected for their ability to drive specific results in their end use application (e.g., uptake of specific nutrients like P or K) based on the known activity of the molecule, or molecular group, in question.
  • Industrial humic substances have little, if any, batch- to-batch variation.
  • Non-Industrial or Commercial humic substances are defined as a preparation of molecules from the humic molecular family that are produced using processes which target this molecular family in general, without consideration for specific molecular components.
  • the separation of molecular components is based on a set of pH and solubility/precipitation relationships. These molecular mixtures may be selected for their ability to drive generalized results in the end-use application (e.g., growth promotion, nutrient uptake). Non-Industrial or Commercial humic substances often have significant batch-to-batch variation.
  • Exemplary humic substances can include humic acid, fulvic acid, humin and/or an industrial humic substance.
  • humic acid refers a to an organic composition, often extracted from humas found in soil, which is typically soluble in water at a pH of 7 or above and insoluble at a pH of 2 or below.
  • Humic acids may comprise carboxylates and/or phenolate groups which can form complexes with ions in the soil and have been shown to play a role in regulating the bioavailability of metal ions in soil. See Lovland PJ., Humic Substances: Structures, Models and Functions, The Journal of Agricultural Science. 2002; 139(1): 113-114., the relevant disclosures of which are incorporated by reference for the purpose and subject matter referenced herein.
  • fulvic acid refers to an organic composition, often extracted from humus found in soil, which shares many structural similarities to humic acid, but in general has a higher number of carboxylic groups and a lower molecular weight. The structure of fulvic acid allows for it to be soluble in water at any pH.
  • humin refers to an organic composition, often extracted from humus found in soil, which is not soluble in water at any pH and, when compared to humic acid and fulvic acid, generally has a higher molecular weight and lower levels of functional groups, specifically carboxyl and hydroxyl. See Hayes M et al., Chapter Two -Humin: Its Composition and Importance in Soil Organic Matter, Advan. Agron., 2017; 4:47-138.
  • seaweed extract can be understood to mean a liquid extract or a dried/pulverized extract of one or more varieties of seaweed.
  • Seaweed may be understood to mean a multicellular macroalgae.
  • the seaweed variety can be a red seaweed/alga (Rhodophyta), a green seaweed/alga (Chlorophyta), or a brown seaweed/alga (Phaeophyceae, e.g. kelp).
  • the seaweed extract may comprise one or more macro-nutrients, micro-nutrients, humic substances including humic acids, phytohormones, soluble alginates, and combinations thereof.
  • the seaweed extract may be used as fertilizer and is commercially available (e.g. Kelpak®). Suitable seaweed extracts would be known to a person of ordinary skill in the art.
  • biologically stable refers to the microbial titer of a microbial composition changing no more than 25% (e.g., no more than 20%, no more than 15%, or no more than 10%) upon storage at a specific temperature (e.g. at room temperature) for a period of time (e.g., at least 6 months).
  • biological performance may include, but is not limited to, product stability, and/or the ability of a microbial consortium to retain a concentration of viable, culturable endospores that meets or exceeds the product's label claim.
  • nutrient use efficiency may also refer to fertilizer use efficiency (FUE).
  • FUE fertilizer use efficiency
  • Tl is a control treatment (e.g. urea) and “Tx” is a unique treatment condition.
  • the nutrient applied can be Nitrogen, Phosphorus, and Potassium, among others.
  • Yield can be understood to mean the amount of agricultural production harvested per unit area of land. It can also be understood to mean grain yield (e.g. corn grain yield) or seed ratio (e.g. the multiplication ratio between the number of plants harvested for each seed planted).
  • the term “Vegetation Index” can be understood to mean a spectral imaging transformation of two or more image bands to enhance the contribution of certain green vegetative features allowing for them to be distinguished from other objects in the image.
  • the Vegetation Index can be a simple ratio (SR) which is a ratio between the reflectance recorded in Near Infrared (NIR) and Red bands.
  • the Vegetation Index can be a Normalized Difference Vegetation Index (ND VI) which is a method to monitor of green cover in an area calculated by taking a ratio between the difference and the sum of the reflectance in the NIR and Red bands.
  • the Vegetation Index can be a Photochemical Reflectance Index as a measure of light usage which is a measure of light-use efficiency.
  • Standard fertilizer program can be understood to mean a program comprising treatment with urea.
  • a reference to “A and/or B”, when used in conjunction with open-ended language such as “comprising” may refer, in one embodiment, to A only (optionally including elements other than B); in another embodiment, to B only (optionally including elements other than A); in yet another embodiment, to both A and B (optionally including other elements); etc.
  • the phrase “at least one,” in reference to a list of one or more elements, should be understood to mean at least one element selected from any one or more of the elements in the list of elements, but not necessarily including at least one of each and every element specifically listed within the list of elements and not excluding any combinations of elements in the list of elements.
  • This definition also allows that elements may optionally be present other than the elements specifically identified within the list of elements to which the phrase “at least one” refers, whether related or unrelated to those elements specifically identified.
  • “at least one of A and B” may refer, in one embodiment, to at least one, optionally including more than one, A, with no B present (and optionally including elements other than B); in another embodiment, to at least one, optionally including more than one, B, with no A present (and optionally including elements other than A); in yet another embodiment, to at least one, optionally including more than one, A, and at least one, optionally including more than one, B (and optionally including other elements); etc.
  • the invention can be understood with reference to the following enumerated embodiments.
  • Embodiment 1 A composition comprising at least one bacterial additive and at least one humic substance, which supports stability and viability of the bacterial additive while improving biological efficiency of the product.
  • Embodiment 2 The composition of embodiment 1, wherein the at least one bacterial additive and at least one humic substance may be coupled with delivery vehicles including, but not limited to, mineral fertilizer carriers.
  • Embodiment 3 A composition comprising at least one bacterial additive and at least one humic substance coated onto a mineral fertilizer carrier which will maintain or improve bacterial viability and/or adherence to the fertilizer carrier under environmental conditions.
  • Embodiment 4. A composition comprising at least one spore forming bacterial additive and at least one humic substance.
  • Embodiment 5 The compositions of any one of the previous embodiments, wherein the composition is used as microbial-based biostimulant or biofertilizer product to support stability and viability of the bacterial additive while improving biological efficiency of the product, while supporting specialty usages such as increased nutrient use efficiency and/or crop nutrient availability.
  • Embodiment 6 The composition of any one of the previous embodiments, wherein the at least one bacterial additive comprises endospore forming Bacillota.
  • Embodiment 7 The composition of any one of the previous embodiments, wherein the at least one bacterial additive comprises Bacillus spp, Brevibacillus spp, Paenibacillus spp, and/or Lysinibacillus spp.
  • Embodiment 8 The composition of any one of the previous embodiments, wherein the at least one humic substance comprises humic acid, fulvic acid and/or humins.
  • Embodiment 9 The composition of any one of the previous embodiments, wherein the composition is blended with a liquid fertilizer or coated on a dry fertilizer.
  • Embodiment 10 The composition of any one of the previous embodiments, wherein the composition is coated onto seeds of any plant.
  • Embodiment 11 The composition of embodiment 9, wherein the liquid fertilizer comprises urea, ammonium nitrate, polyphosphate, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, NPK (blended and compounded), a flowable suspension for fertigation and/or any fertilizer capable of being dissolved.
  • the liquid fertilizer comprises urea, ammonium nitrate, polyphosphate, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, NPK (blended and compounded), a flowable suspension for fertigation and/or any fertilizer capable of being dissolved.
  • Embodiment 12 The composition of embodiment 9, wherein the dry fertilizer comprises urea, monoammonium phosphate, diammonium phosphate, potassium chloride, super simple phosphate, super triple phosphate, potassium nitrate, potassium sulfate, and/or NPK (blended and compounded).
  • Embodiment 13 The composition of embodiment 9, wherein the liquid fertilizer or dry fertilizer comprises nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S), magnesium (Mg), iron (Fe), boron (B), chlorine (Cl), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), nickel (Ni), cobalt (Co) or any combination thereof.
  • Embodiment 14 Embodiment 14.
  • composition of any one of the previous embodiments, wherein the composition comprises the at least one bacterial additive and the at least one humic substance present at a ratio of 1 : 10 to 10: 1; and wherein the at least one bacterial additive comprises Bacillus spp, Brevibacillus spp, Paenibacillus spp, and/ or Lysinibacillus spp.
  • Embodiment 15 The composition of any one of the previous embodiments, wherein the composition improves the biological performance of the bacterial additive.
  • Embodiment 16 The composition of any one of the previous embodiments, wherein the composition improves crop nutrient assimilation, optimizes yield potential, enhances root development, improves plant vigor, enhances native microbial activity in the soil, improves soil productivity and/or improves soil health.
  • Embodiment 17 The composition of any one of the previous embodiments, wherein the composition increases survivability of the bacterial additive.
  • Embodiment 18 The composition of any one of the previous embodiments, wherein the composition increases: a) calcium stability of calcium contained on the coated dry fertilizer; b) magnesium stability of magnesium contained on the coated dry fertilizer; c) manganese stability of manganese contained on the coated dry fertilizer; d) nitrogen stability of nitrogen contained on the coated dry fertilizer; e) phosphorus stability of the phosphorus contained on the coated dry fertilizer; f) zinc crop uptake when applied with zinc contained on the coated dry fertilizer; and/or g) iron crop uptake when applied with iron contained on the coated dry fertilizer.
  • Embodiment 19 The composition of any one of the previous embodiments, wherein the composition improves calcium, magnesium, manganese, nitrogen, potassium, iron, and/or zinc plant uptake in most soils.
  • Embodiment 20 The composition of any one of the previous embodiments, wherein the composition increases survivability of a bacterial spore on a hostile surface.
  • Embodiment 21 The composition of embodiment 20, wherein the hostile surface comprises very high pH (basic), very low pH (acidic), very high level of chemicals that inhibit life, high levels of incomplete reactions that produced chemicals, urea, single super phosphate, and/or super triple phosphate.
  • Embodiment 22 The composition of any one of the previous embodiments, wherein the composition further comprises a surfactant and/or a wetter agent.
  • Embodiment 23 The composition of any one of the previous embodiments, wherein the composition humidity is below 20%.
  • Embodiment 24 A method of using a composition comprising at least one bacterial additive and at least one humic substance, wherein the method comprises a specialty usage.
  • Embodiment 24 The method of embodiment 24, wherein the specialty usage comprises increased nutrient use efficiency and/or crop nutrient availability.
  • Embodiment 26 The method of embodiment 24, wherein the nutrient comprises nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, non-essential nutrients, and/or micronutrients.
  • Embodiment 27 The method of embodiment 26, wherein the non-essential nutrients comprise titanium, selenium, and/or silicon.
  • Exemplary composition BW Fl comprises Bacillus amyloliquefaciens (1.25E+09 CFU/mL, or colony forming units per milliliter), Bacillus subtilis (1.25E+09 CFU/mL), Bacillus pumilus (1.25E+09 CFU/mL), and Bacillus licheniformis (1.25E+09 CFU/mL), for a total Bacillus content of 5.00E+09 CFU/mL.
  • composition BW F2 comprises Bacillus amyloliquefaciens (1.25E+09 CFU/mL), Bacillus pumilus (1.25E+09 CFU/mL), and Bacillus licheniformis (1.25E+09 CFU/mL), for a total Bacillus content of 3.75E+09 CFU/mL.
  • the compositions of the present invention may be made by any of the standard processes known in the art. Table 1 - Composition and treatment details. * kg/ha refers to kilograms per hectare
  • T2 Untreated control (UTC) FERTILITY LEVEL - 100% Urea (sometimes referred to as 100% N, i.e. with urea applied at 200 kg nitrogen (N) per hectare), (T2) FERTILITY LEVEL - 100% Urea + HUMIC S, (T3) FERTILITY LEVEL - 100% Urea BW Fl,
  • T7 FERTILITY LEVEL - 75% Urea (sometimes referred to as 75% N, i.e. with urea applied at 150 kg N per hectare) HUMIC S,
  • Plant materials (leaf sample opposite and below the ear) were collected at silking stage and analyzed for nutrients content such as N, P, K, calcium (Ca), magnesium (Mg), sulfur (S), sodium (Na), copper (Cu), zinc (Zn), manganese (Mn), iron (Fe)_, and boron (B). Soil and corn grain sample were also analyzed at harvest stage for several parameters. Corn grain yield was recorded at harvest. [00143] The effect of treatments on corn yield was assessed. The yield of corn ranged between 6.85 to 7.97 kg/plot (Table 2 and Figure 1).
  • BW Fl in combination with urea FERTILITY LEVEL - 100% + HUMIC S (T4), BW F2 + Urea FERTILITY LEVEL - 100% (T5) and BW F2 with Urea FERTILITY LEVEL - 100% + HUMIC S (T6) are suitable for improving productivity of corn. Further, application of N at the 100% level was found to be superior to that of N at the 75% level. However, even at 75% level of N, use of BiOWiSH products containing BW Fl and BW F2 resulted in significantly higher corn yields than the products without BW Fl and BW F2.
  • compositions of the present invention resulted in the improvement of the crop yield of com.
  • the improvement in crop yield of com may be the culmination of efficient utilization of applied N through these compositions as well as other applied/available nutrients in the soil.
  • results related to the yield of com grain demonstrated the significant positive effect of different compositions of the present inventions (BiOWiSH Technologies Formulation + Humic-S with urea coated with BiOWiSH® Crop Liquid containing biofertilizers).

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Fertilizers (AREA)

Abstract

L'invention concerne des compositions comprenant au moins un additif bactérien, tel qu'une espèce Bacillota, et au moins une substance humique. Les compositions peuvent être combinées à des engrais, et peuvent être utilisées dans des procédés permettant d'augmenter l'efficacité d'utilisation des nutriments et/ou la disponibilité des nutriments de culture dans des plantes.
PCT/US2024/022622 2023-04-03 2024-04-02 Compositions de micro-organisme formant des spores avec des substances humiques et procédés d'utilisation associés WO2024211278A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363493903P 2023-04-03 2023-04-03
US63/493,903 2023-04-03

Publications (1)

Publication Number Publication Date
WO2024211278A1 true WO2024211278A1 (fr) 2024-10-10

Family

ID=91029919

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/022622 WO2024211278A1 (fr) 2023-04-03 2024-04-02 Compositions de micro-organisme formant des spores avec des substances humiques et procédés d'utilisation associés

Country Status (1)

Country Link
WO (1) WO2024211278A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100275663A1 (en) * 2001-12-31 2010-11-04 Microbes, Inc. Fertilizer compositions and methods of making and using same
US20190169078A1 (en) * 2017-10-17 2019-06-06 Khanh Le Microbial humic soil enhancements
US20200055793A1 (en) * 2017-10-17 2020-02-20 Khanh Le Soil enhancement
WO2020247824A1 (fr) * 2019-06-06 2020-12-10 Loveland Products, Inc. Formulations agricoles et leurs procédés de fabrication et d'utilisation
WO2021022128A1 (fr) 2019-08-01 2021-02-04 BiOWiSH Technologies, Inc. Composition microbienne aqueuse stable

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100275663A1 (en) * 2001-12-31 2010-11-04 Microbes, Inc. Fertilizer compositions and methods of making and using same
US20190169078A1 (en) * 2017-10-17 2019-06-06 Khanh Le Microbial humic soil enhancements
US20200055793A1 (en) * 2017-10-17 2020-02-20 Khanh Le Soil enhancement
WO2020247824A1 (fr) * 2019-06-06 2020-12-10 Loveland Products, Inc. Formulations agricoles et leurs procédés de fabrication et d'utilisation
WO2021022128A1 (fr) 2019-08-01 2021-02-04 BiOWiSH Technologies, Inc. Composition microbienne aqueuse stable

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
AMPONG ET AL.: "Understanding the Role of Humic Acids on Crop Performance and Soil Health", FRONT. AGRON, vol. 4, 2022
ANONYMOUS: "BiOWiSH Crop Organic Soluble Solid Microbial Solution", BIOWISH CROP ORGANIC BROCHURE, 20 April 2020 (2020-04-20), Cincinnati, OH USA, pages 1 - 2, XP093179886, Retrieved from the Internet <URL:http://b14279a71a966e52ae36-fad5b9385501cb7f7cac4f09f1c56222.r14.cf5.rackcdn.com/Brochures/Crop%20Organic%20Flyer%201595-03-EN.pdf> *
ANONYMOUS: "Evaluation of BiOWiSH Crop on Early-Stage Sweet Corn", RESEARCH STUDY BIOWISH CROP, 8 August 2017 (2017-08-08), Cincinnati, OH USA, pages 1 - 5, XP093179890, Retrieved from the Internet <URL:http://b14279a71a966e52ae36-fad5b9385501cb7f7cac4f09f1c56222.r14.cf5.rackcdn.com/Research%20Studies/Agronomy/1179-02-EN%20Evaluation%20of%20BiOWiSH%20Crop%20Benefits%20on%20Sweet%20Corn.pdf> *
DA SILVA MAURA SANTOS REIS DE ANDRADE ET AL: "Humic Substances in Combination With Plant Growth-Promoting Bacteria as an Alternative for Sustainable Agriculture", FRONTIERS IN MICROBIOLOGY, vol. 12, 29 October 2021 (2021-10-29), Lausanne, XP093146103, ISSN: 1664-302X, Retrieved from the Internet <URL:https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.719653/full> DOI: 10.3389/fmicb.2021.719653 *
FILHO JOSE FRANCO ET AL: "The Synergistic Effects of Humic Substances and Biofertilizers on Plant Development and Microbial Activity: A Review", INTERNATIONAL JOURNAL OF PLANT & SOIL SCIENCE, 24 June 2020 (2020-06-24), pages 56 - 75, XP093180089, ISSN: 2320-7035, Retrieved from the Internet <URL:http://www.journalijpss.com/index.php/IJPSS/article/download/30306/56860> DOI: 10.9734/ijpss/2020/v32i730306 *
HAYES M ET AL.: "Two -Humin: Its Composition and Importance in Soil Organic Matter", ADVAN. AGRON., vol. 4, 2017, pages 47 - 138
LOVLAND PJ.: "Humic Substances: Structures, Models and Functions", THE JOURNAL OF AGRICULTURAL SCIENCE, vol. 139, no. 1, 2002, pages 113 - 114
RATANAPROMMANEE CHANYUT ET AL: "Chemical characterization of leonardite and its potential use as soil conditionerand plant growth enhancement", ASIA-PACIFIC JOURNAL OF SCIENCE AND TECHNOLOGY, vol. 22, no. 04, 29 December 2017 (2017-12-29), pages 1 - 10, XP093180020, Retrieved from the Internet <URL:https://so01.tci-thaijo.org/index.php/APST/article/view/107637> *
SCHOEBITZ MAURICIO ET AL: "Combined application of microbial consortium and humic substances to improve the growth performance of blueberry seedlings", JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, no. ahead, 1 January 2016 (2016-01-01), pages 0 - 0, XP093180088, ISSN: 0718-9516, Retrieved from the Internet <URL:https://www.scielo.cl/pdf/jsspn/v16n4/aop7416.pdf> DOI: 10.4067/S0718-95162016005000074 *
TREVISAN S. ET AL.: "Humic substances biological activity at the plant-soil interface: from environmental aspects to molecular factors", PLANT SIGNAL BEHAV., vol. 5, no. 6, June 2010 (2010-06-01), pages 635 - 43

Similar Documents

Publication Publication Date Title
Tarigan Utilization azolla liquid organic fertilizer with goat manure to enhance organic okra (Abelmoschus esculentus L. Moench) production
JP3793578B2 (ja) 液体の土壌強化微生物組成物
CN102781254B (zh) 有机螯合的矿物组合物及其制备方法
CN107532139A (zh) 微生物聚生体
CN107787360B (zh) 微生物聚生体
Öğüt et al. Single and double inoculation with Azospirillum/Trichoderma: the effects on dry bean and wheat
CN107849516A (zh) 微生物聚生体
Revolti et al. Azospirillum spp. potential for maize growth and yield
Selvakumar et al. Hairy vetch, compost and chemical fertilizer management effects on red pepper yield, quality, and soil microbial population
Adajar et al. Application of foliar biofertilizers with and without NPK in cultivating white-glutinous corn
CN107827488A (zh) 一种液态复合微生物菌肥及其制备方法
CN105061068B (zh) 一种辣木多糖富集生物液体肥料及其制备方法
WO2018222214A1 (fr) Compositions d&#39;engrais liquides comprenant du nickel, du cobalt et du molybdène, et leurs procédés de formation et d&#39;utilisation
Trimurtulu et al. Liquid microbial inoculants and their efficacy on field crops, ANGRAU
CN108610182A (zh) 一种具有防病促生长功能的复合肥料及其制备方法
Sharma et al. Microbial inoculants: Recent progress in formulations and methods of application
WO2024211278A1 (fr) Compositions de micro-organisme formant des spores avec des substances humiques et procédés d&#39;utilisation associés
Sulok et al. Use of organic soil amendments to improve soil health and yield of immature pepper (Piper nigrum L.)
Priyadi et al. The development of inorganic fertilizer and bio-fertilizer combination and the effectiveness of application on the growth and production of red chili
US20230217929A1 (en) Whole cell methanotroph based biostimulant compositions, methods and applications thereof
Bahri et al. Liquid Organic Fertilizer Test and Tuber Cutting on Shallots (Allium Ascalonicum L.) Growth and Yield
Skamarokhova et al. The role of Foschami bio-fertilizer in increasing the yield of green mass of vetch-wheat grass mixture
Devi et al. Organic Remobilization of zinc and phosphorus availability to plants by application of mineral solubilizing bacteria Pseudomonas aeruginosa
Rao Promoting biofertilizers in IPNS with improved technology and extension in India
Radovich et al. Innovative use of locally produced inputs to improve plant growth, crop quality, and grower profitability in Hawai’i