WO2024082187A1

WO2024082187A1 - Pipetting apparatus for fluidic chip, and pipetting method for fluidic chip

Info

Publication number: WO2024082187A1
Application number: PCT/CN2022/126278
Authority: WO
Inventors: 要军磊; 张萌萌; 张宇宁; 云全新; 黎宇翔; 董宇亮; 章文蔚; 徐讯
Original assignee: 深圳华大生命科学研究院
Priority date: 2022-10-19
Filing date: 2022-10-19
Publication date: 2024-04-25

Abstract

The present invention relates to the technical field of biological chips. Provided are a pipetting apparatus for a fluidic chip, and a pipetting method for a fluidic chip. The pipetting apparatus for a fluidic chip comprises a working platform, a moving component, a pipetting device and a drying device, wherein the working platform comprises a fluidic-chip placement area; the moving component can move above the working platform; the pipetting device is arranged on the moving component and is used for performing liquid injection and/or liquid suction on fluidic chips; and the drying device is used for injecting gas into flow channels of the fluidic chips. By means of adding the drying device so as to accelerate the circulation of the gas inside the fluidic chips, the drying efficiency for the fluidic chips is improved, such that the drying time is greatly shortened, thereby avoiding the residue of liquids in the flow channels of the fluidic chips; moreover, a pipetting operation is carried out by means of the moving component driving the pipetting device, such that there is no need to manually use a pipette to carry out the pipetting operation, thereby preventing operators from coming into contact with chemicals, and achieving a high degree of automation and making the present invention safer.

Description

Fluid chip liquid transfer device and liquid transfer method of fluid chip

Technical Field

The present invention relates to the technical field of biochips, and in particular to a fluid chip liquid transfer device and a liquid transfer method for a fluid chip.

Background technique

Microfluidics has important applications in sequencing technology, and fluid chips are the main platform for implementing microfluidics. Due to the small flow channel and complex structure, liquid residue is easily left during liquid replacement, affecting the reagent replacement effect. The current liquid transfer equipment has a relatively single function, with only the liquid filling part, and the drying is mostly static evaporation, resulting in poor drying effect.

Summary of the invention

The technical problem to be solved by the present invention is to overcome the defects of liquid residue and poor drying effect in the fluid chip in the prior art, and to provide a fluid chip pipetting device and a fluid chip pipetting method.

The present invention solves the above technical problems through the following technical solutions:

A fluid chip liquid transfer device, comprising:

A working platform, the working platform comprising a fluid chip placement area;

A moving component capable of moving above the working platform;

A liquid transfer device, which is disposed on the moving component and is used for injecting and/or aspirating liquid from the fluid chip;

A drying device is used to inject gas into the flow channel of the fluid chip.

In this scheme, by adding a drying device, the circulation of gas inside the fluid chip is accelerated, the drying efficiency of the fluid chip is improved, the drying time is greatly shortened, and the residue of liquid in the flow channel of the fluid chip is avoided. In addition, the pipetting operation is performed by driving the pipetting device through moving parts. The pipetting does not require manual operation using a pipette gun, which avoids the contact between the operator and chemicals. It has a high degree of automation and is safer.

Preferably, the moving component comprises a first robotic arm, the first robotic arm has at least three degrees of freedom and can move along three directions of X, Y and Z, and the pipetting device is disposed on the first robotic arm.

In this solution, the first robotic arm has a high degree of freedom and is more flexible.

Preferably, the work platform further comprises a pipette tip consumables area, a reagent storage area and a pipette tip recovery area, and the first robot arm can move among the pipette tip consumables area, the reagent storage area, the pipette tip recovery area and the fluid chip placement area.

In this solution, the first robot moves along the Z direction, which can drive the pipette device to take the tip, absorb liquid, inject liquid, and withdraw the tip. The first robot moves along the X and Y directions to drive the pipette device to move between the tip consumables area, the reagent storage area, the tip recovery area, and the fluid chip placement area. In addition, by setting up the tip consumables area, the pipette device can be used with disposable tips, which can effectively avoid cross contamination.

Preferably, the moving component includes a second robotic arm, the second robotic arm has at least three degrees of freedom and can move in three directions of X, Y and Z, the second robotic arm can move in the fluid chip placement area, and the drying device is arranged on the second robotic arm.

In this solution, driven by the second robot arm, the drying device can move in the fluid chip placement area, and then each fluid chip can be dried, with higher drying efficiency.

Preferably, the moving component is a robotic arm, which has at least three degrees of freedom and can move along three directions of X, Y and Z, and the liquid transfer device and the drying device are arranged on the robotic arm.

In this solution, by arranging the pipetting device and the drying device on one robotic arm, it is helpful to reduce costs, and the pipetting device and the drying device will not interfere with each other during the movement, and the two can work at the same time.

Preferably, the drying device comprises an air path device, and the air path device is arranged on the moving component.

In this solution, the air path device is used for drying, which is beneficial to improve the drying efficiency and will not affect the biochemical reaction characteristics inside the fluid chip. The air path device can move with the moving parts, and thus the fluid chip can be dried in a targeted manner with higher efficiency.

Preferably, the air path device comprises an air pump, an air outlet pipe and a nozzle, one end of the air outlet pipe is connected to the air outlet of the air pump, and the other end of the air outlet pipe is connected to the nozzle.

In this solution, the air pump can generate air pressure, which can increase the air flow velocity at the nozzle, thereby improving the drying efficiency of the flow channel in the fluid chip.

Preferably, the nozzle is an air blowing suction cup.

In this solution, an air blowing suction cup is used. On the one hand, the air blowing suction cup can fit more closely with the flow channel inlet of the fluid chip, and the connection reliability is high. On the other hand, the air blowing suction cup can be replaced with air ports of different sizes, and its application range is wider. The air outlet area is adjustable and can adapt to flow channels of different apertures.

Preferably, the air path device further comprises an air intake pipe and a filter, one end of the air intake pipe is connected to the air inlet of the air pump, and the other end of the air intake pipe is connected to the filter.

In this solution, by adding a filter, the cleanliness of the air entering the flow channel of the fluid chip is ensured, thereby preventing contamination of the fluid chip.

Preferably, the gas path device further comprises a flow mechanism, and the flow mechanism is arranged on the gas outlet pipe.

In this solution, the flow mechanism can detect the working status in real time and adjust different flow rates according to demand.

Preferably, the fluid chip placement area is provided with a fluid chip placement platform and a fixing portion, the fluid chip placement platform is detachably arranged in the fixing portion, and a plurality of fluid chips are arranged on the fluid chip placement platform.

In this solution, the fluid chip placement platform is placed more stably and is less likely to shift, and the fluid chip placement platform can be replaced to adapt to fluid chips of different structures.

Preferably, the fluid chip pipetting device is used for sequencing.

In this scheme, liquid residue is less likely to remain inside the flow of the fluid chip, sequencing is less affected by interference factors, and the sequencing results are more accurate.

A fluid chip pipetting method, which uses the fluid chip pipetting device as described above, and the pipetting method comprises:

The moving component drives the liquid transfer device to inject liquid into the fluid chip;

The moving component drives the liquid transfer device to aspirate liquid from the fluid chip;

The drying device blows air to dry the fluid chip.

The method is used for pipetting, and the pipetting does not need to be operated manually using a pipette gun, thus avoiding contact between operators and chemicals. The degree of automation is high, and the drying efficiency of the flow channel of the fluid chip can be accelerated by a drying device to avoid liquid residue.

Preferably, the step of the moving component driving the liquid transfer device to inject liquid into the fluid chip specifically includes the following steps:

S11, the moving component drives the pipetting device to move to the pipette tip consumables area, and drives the pipetting device to descend to obtain the pipette tip;

S12, the moving component drives the liquid transfer device to move to the reagent storage area, and the liquid transfer device absorbs the reagent;

S13, the moving component drives the liquid transfer device to move to the fluid chip placement area, and the liquid transfer device injects liquid into the fluid chip;

S14, the moving component drives the pipetting device to move to the tip recovery area, and the pipetting device discards the tip.

Preferably, the step of the moving component driving the liquid transfer device to aspirate the fluid chip specifically includes the following steps:

S21, the moving component drives the pipetting device to move to the pipette tip consumables area, and the pipetting device descends to obtain the pipette tip;

S22, the moving component drives the liquid transfer device to move to the fluid chip placement area, and the liquid transfer device aspirates the fluid chip;

S23, the moving component drives the pipetting device to move to the tip recovery area, and the pipetting device discharges the waste liquid and discards the tip.

Preferably, the step of the drying device blowing air to dry the fluid chip specifically comprises the following steps:

S31, the moving component drives the drying device to move above the chip port of the fluid chip;

S32, the moving device drives the drying device to move downward, so that the air outlet of the drying device is in contact with the chip port;

S33, the drying device blows air to dry the fluid chip;

S34, repeating steps S31 to S33 until the drying device completes the air blowing drying of all fluid chips. The positive and progressive effects of the present invention are: in the present invention, by adding a drying device, the circulation of air inside the fluid chip is accelerated, the drying efficiency of the fluid chip is improved, the drying time is greatly shortened, and the liquid residue in the flow channel of the fluid chip is avoided. In addition, the pipetting operation is carried out by driving the pipetting device with a moving part, and the pipetting does not need to be operated manually using a pipette gun, which avoids the contact between the operator and the chemicals, has a high degree of automation, and is safer.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic structural diagram of a fluid chip liquid transfer device according to a preferred embodiment of the present invention;

FIG2 is a schematic diagram of the structure of a gas path device according to a preferred embodiment of the present invention;

FIG. 3 is a flow chart of a liquid transfer method according to a preferred embodiment of the present invention.

Work Platform 100

Fluid chip placement area 110

Fixing portion 111

Fluid chip placement platform 112

Tip consumables area 120

Reagent storage area 130

Tip recovery area 140

Fluid Chip 200

First Robotic Arm 310

Second robotic arm 320

Liquid handling device 400

Gas circuit device 500

Air Pump 510

Exhaust pipe 520

Flow Mechanism 530

Blowing suction cup 540

Intake pipe 550

Filter 560

Detailed ways

A preferred embodiment is given below, and the present invention is described more clearly and completely in conjunction with the accompanying drawings.

As shown in Figures 1 and 2, this embodiment provides a fluid chip pipetting device, which includes: a working platform 100, a moving part, a pipetting device 400 and a drying device, the working platform 100 includes a fluid chip placement area 110, the moving part can move above the working platform 100, the pipetting device 400 is arranged on the moving part, and is used to inject and/or absorb liquid into the fluid chip 200, and the drying device is used to inject gas into the flow channel of the fluid chip 200.

By adding a drying device, the circulation of gas inside the fluid chip 200 is accelerated, the drying efficiency of the fluid chip 200 is improved, the drying time is greatly shortened, and the residue of liquid in the flow channel of the fluid chip 200 is avoided. In addition, the pipetting operation is performed by driving the pipetting device 400 with a moving part. The pipetting does not require manual operation using a pipette gun, which avoids the contact between the operator and chemicals. It has a high degree of automation and is safer.

In this embodiment, both the pipetting and the air blowing drying are aimed at the chip port of the fluid chip 200 .

Specifically, the fluid chip 200 includes a chip inlet and a chip outlet. When injecting liquid, the pipette device 400 needs to be aligned with the chip inlet, and when aspirating liquid, the pipette device 400 can be aligned with the chip inlet or the chip outlet. When passing gas to dry, the drying device can be aligned with the chip inlet or the chip outlet.

Among them, clean air or inert gas can be used for drying.

Among them, in this embodiment, the pipetting device 400 adopts an ADP air plunger pipetting pump, whose pipetting range is 1μL-1000μL and the pipetting accuracy can reach up to ±1%. The pipetting pump integrates a pressure sensor, which can effectively detect reagent blockage and flow channel blockage problems of the fluid chip 200.

In other embodiments, the liquid transfer device may also be a syringe, a micropump, or other devices with liquid injection and/or liquid aspiration functions.

In other embodiments, multiple pipetting devices may be provided, and providing multiple pipetting devices simultaneously to form a multi-channel layout is beneficial to improving pipetting efficiency, such as providing an 8-channel adjustable pipetting device.

In other embodiments, multiple drying devices may be provided, and multiple drying devices may be provided to form a multi-channel layout, which is beneficial to improving the drying efficiency. As shown in FIG1 , in this embodiment, the moving part includes a first mechanical arm 310, and the first mechanical arm 310 has three degrees of freedom and can move in three directions of X, Y, and Z. The liquid transfer device 400 is provided on the first mechanical arm 310. The first mechanical arm 310 has a high degree of freedom and is more flexible. Of course, in other embodiments, the first mechanical arm 310 may have more than three degrees of freedom.

The work platform 100 further includes a pipette consumables area 120, a reagent storage area 130, and a pipette recovery area 140. The first mechanical arm 310 can move between the pipette consumables area 120, the reagent storage area 130, the pipette recovery area 140, and the fluid chip placement area 110. The first mechanical arm 310 moves back and forth in the Z direction, which can drive the pipette device 400 to take the pipette tip, absorb liquid, inject liquid, and withdraw the pipette tip. The first mechanical arm 310 moves along the X and Y directions to drive the pipette device 400 to move between the pipette consumables area 120, the reagent storage area 130, the pipette recovery area 140, and the fluid chip placement area 110. In addition, by setting the pipette consumables area 120, the pipette device 400 can be used with disposable pipette tips, which can effectively avoid cross contamination.

The reagent storage area 130 can be flexibly configured according to the needs, so as to be able to place centrifuge tubes of different specifications such as 500μL-50mL. The tip consumable area 120 can be used to place three different specifications of tips to meet the needs of different ranges of pipetting. The tip recovery area 140 can be used to place waste buckets, which are convenient for taking out and dumping when the waste bucket is full.

In this embodiment, the moving part may further include a second mechanical arm 320, which has three degrees of freedom and can move in three directions, namely, X, Y and Z. The second mechanical arm 320 can move in the fluid chip placement area 110, and the drying device is disposed on the second mechanical arm 320. Driven by the second mechanical arm 320, the drying device can move in the fluid chip placement area 110, and then can dry each fluid chip 200. By moving in the Z direction, it can fit with the chip inlet or chip outlet of the fluid chip 200, and the drying efficiency is higher. Of course, in other embodiments, the second mechanical arm 320 may have more than three degrees of freedom.

As shown in FIG1 , in this embodiment, a first track is arranged along the X direction, and the first robot 310 and the second robot 320 have the same structure. The first robot 310 includes a supporting body, an X-direction moving part, a Y-direction moving part, and a Z-direction moving part. The supporting body is provided with an X-direction moving part matched with the first track, which can drive the supporting body to move along the X direction. The supporting body is provided with a second track along the Y direction, and the Y-direction moving part is arranged on the second track and can move along the Y direction. The Y-direction moving part is provided with a third track along the Z direction, and the Z-direction moving part is arranged on the third track and can move along the Z direction. Among them, the liquid transfer device is connected to the Z-direction moving part.

In other embodiments, the drying device and the liquid transfer device may be replaced by a clamping device, a capping device, a code scanning device, etc. to achieve corresponding functions. Alternatively, a clamping device, a capping device, a code scanning device, etc. may be added to the liquid transfer device to achieve more functions.

In addition, modules such as mixing, shaking, and magnetic rack may be added to the working platform 100 .

In other embodiments, the layout of various areas on the work platform may be changed to meet corresponding needs.

In other embodiments, the fluid chip pipetting device can also be used in the field of reagent packaging.

In other embodiments, the moving part may be only a mechanical arm, which has three degrees of freedom and can move in three directions, X, Y, and Z, and the liquid transfer device and the drying device are arranged on the mechanical arm. By arranging the liquid transfer device 400 and the drying device on one mechanical arm, it is helpful to reduce the cost, and the liquid transfer device 400 and the drying device will not interfere with each other during the movement, and the two can work at the same time. Among them, the degrees of freedom of the mechanical arm can also be more than three.

In this embodiment, the drying device includes an air circuit device 500, which is arranged on the moving part. Using the air circuit device 500 for drying is conducive to improving the drying efficiency and will not affect the biochemical reaction characteristics inside the fluid chip 200. The air circuit device 500 can move with the moving part, and then the fluid chip 200 can be dried in a targeted manner, which is more efficient.

In other embodiments, the drying device may not be provided with the air path device 500, and drying may be performed by using a fan.

In this embodiment, as shown in FIG2 , the air path device 500 includes an air pump 510, an air outlet pipe 520 and a nozzle, one end of the air outlet pipe 520 is connected to the air outlet of the air pump 510, and the other end of the air outlet pipe 520 is connected to the nozzle. The air pump 510 can generate air pressure, which can increase the air flow velocity at the nozzle, thereby improving the drying efficiency of the flow channel in the fluid chip 200.

In other embodiments, the air path device 500 may not be disposed on a moving component, and drying may be performed by changing the jet angle of the nozzle.

Specifically, in this embodiment, the nozzle is an air blowing suction cup 540. The air blowing suction cup 540 can be more closely attached to the flow channel inlet of the fluid chip 200, and the connection reliability is high. On the other hand, the air blowing suction cup 540 can be replaced with air ports of different sizes, and its application range is wider, and the air outlet area is adjustable, and it can adapt to flow channels of different apertures.

In this embodiment, the air path device 500 further includes an air inlet pipe 550 and a filter 560. One end of the air inlet pipe 550 is connected to the air inlet of the air pump 510, and the other end of the air inlet pipe 550 is connected to the filter 560. By adding the filter 560, the cleanliness of the air entering the flow channel of the fluid chip 200 is ensured, and contamination of the fluid chip 200 is prevented.

In other embodiments, when the cleanliness of the surrounding air is relatively high, the air intake pipe and the filter may not be provided.

In this embodiment, the gas circuit device 500 further includes a flow mechanism 530, which is disposed on the gas outlet pipe 520. The flow mechanism 530 can detect the working state in real time and adjust different flow rates according to requirements.

Specifically, the flow mechanism may be a flow meter or other components with flow monitoring and regulating functions.

As shown in FIG1 , in this embodiment, the fluid chip placement area 110 is provided with a fluid chip placement platform 112 and a fixing portion 111, the fluid chip placement platform 112 is detachably arranged in the fixing portion 111, and a plurality of fluid chips 200 are arranged on the fluid chip placement platform 112. The placement of the fluid chip placement platform 112 is more stable and less likely to shift, and the fluid chip placement platform 112 can be replaced, thereby being able to adapt to fluid chips 200 of different structures and sizes.

In this embodiment, the fluid chip liquid transfer device is used for sequencing, more specifically, for single molecule sequencing. Liquid residue is not easy to exist in the flow of the fluid chip 200, single molecule sequencing is less disturbed by factors, and the sequencing result is more accurate.

In this embodiment, the fluid chip 200 pipetting device also includes a control system, which includes a host computer, connecting lines and a driver. Through the electrical connection between the host computer and the driver, and through communication between the host computer and the driver, the valves of the first robotic arm 310, the second robotic arm 320 and the air pump 510 can be controlled separately, or they can be jointly controlled through autonomous timing to reduce manual participation. The number of control channels can be freely selected, and the number of operations and the cycle mode can be defined by the host computer to achieve fully automated control means.

Among them, the upper computer can send logical instructions through high-speed data lines to communicate with the driver, and use handshake signal judgment, data verification and other methods to ensure the correctness of the instructions sent, so that the driver can control the corresponding components to achieve automatic liquid suction, automatic liquid injection, automatic air blowing and other actions, ensuring the accuracy of the entire process without human intervention. All components are electronically controlled and can be operated with one button on the upper computer, which is convenient and fast, and the injection time, injection speed and injection volume can be precisely controlled, with high stability, which can effectively avoid human errors.

In addition, as shown in FIG3 , this embodiment further provides a fluid chip pipetting method, which uses the fluid chip pipetting device as above, and the pipetting method includes:

S10, the moving component drives the liquid transfer device to inject liquid into the fluid chip.

S20, the moving component drives the liquid transfer device to aspirate liquid from the fluid chip.

S30, the drying device blows air to dry the fluid chip.

In other embodiments, before using the fluid chip for reaction, step S30 may be performed first to remove dust in the flow channel and keep the flow channel dry. If there is residual liquid in the fluid chip, step S20 may be performed first to absorb the residual liquid.

In other embodiments, step S20 and step S30 may also be performed simultaneously. When cleaning the fluid chip, the drying efficiency can be improved by performing air blowing while draining the waste liquid in the flow channel in step S20.

In this embodiment, S10 specifically includes:

S11, the moving component drives the pipetting device to move to the pipette tip consumables area, and drives the pipetting device to descend to obtain the pipette tip.

S12, the moving component drives the liquid transfer device to move to the reagent storage area, and the liquid transfer device absorbs the reagent.

S13, the moving component drives the pipetting device to move to the fluid chip placement area, and the pipetting device injects liquid into the fluid chip.

In this embodiment, S20 specifically includes:

S21, the moving component drives the pipetting device to move to the pipette tip consumables area, and the pipetting device descends to obtain the pipette tip.

S22, the moving component drives the pipetting device to move to the fluid chip placement area, and the pipetting device aspirates liquid from the fluid chip.

S23, the moving component drives the liquid transfer device to move to the tip recovery area, and the liquid transfer device discharges the waste liquid and discards the tip.

In this embodiment, S30 specifically includes:

S32, the moving device drives the drying device to move downward so that the air outlet of the drying device fits with the chip opening;

S33, the drying device blows air to dry the fluid chip;

S34, repeat steps S31 to S33 until the drying device completes the air blowing drying of all fluid chips.

Although the specific embodiments of the present invention are described above, it should be understood by those skilled in the art that this is only for illustration and the protection scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims

A fluid chip pipetting device, characterized in that it comprises:

A working platform, the working platform comprising a fluid chip placement area;

A moving component capable of moving above the working platform;

A liquid transfer device, which is disposed on the moving component and is used for injecting and/or aspirating liquid from the fluid chip;

A drying device is used to inject gas into the flow channel of the fluid chip.
The fluid chip pipetting device as described in claim 1 is characterized in that the moving component includes a first robotic arm, the first robotic arm has at least 3 degrees of freedom and can move in three directions of X, Y, and Z, and the pipetting device is arranged on the first robotic arm.
The fluid chip pipetting device as described in claim 2 is characterized in that the working platform also includes a tip consumable area, a reagent storage area and a tip recovery area, and the first robotic arm can move between the tip consumable area, the reagent storage area, the tip recovery area and the fluid chip placement area.
The fluid chip pipetting device as described in at least one of claims 1 to 3 is characterized in that the moving part includes a second robotic arm, the second robotic arm has at least 3 degrees of freedom and can move in three directions of X, Y, and Z, the second robotic arm can move in the fluid chip placement area, and the drying device is arranged on the second robotic arm.
The fluid chip pipetting device as described in at least one of claims 1 to 3 is characterized in that the moving part is a robotic arm, the robotic arm has at least 3 degrees of freedom and can move in three directions of X, Y, and Z, and the pipetting device and the drying device are arranged on the robotic arm.
The fluid chip pipetting device as described in at least one of claims 1 to 5 is characterized in that the drying device includes an air path device, and the air path device is arranged on the moving part.
The fluid chip pipetting device as described in claim 6 is characterized in that the air path device includes an air pump, an air outlet pipe and a nozzle, one end of the air outlet pipe is connected to the air outlet of the air pump, and the other end of the air outlet pipe is connected to the nozzle.
The fluid chip pipetting device as described in claim 7 is characterized in that the nozzle is an air blowing suction cup.
The fluid chip pipetting device as described in claim 8 is characterized in that the air path device also includes an air inlet pipe and a filter, one end of the air inlet pipe is connected to the air inlet of the air pump, and the other end of the air inlet pipe is connected to the filter.
The fluid chip pipetting device as described in claim 9 is characterized in that the air path device also includes a flow mechanism, and the flow mechanism is arranged on the air outlet pipe.
The fluid chip pipetting device as described in at least one of claims 1 to 10 is characterized in that the fluid chip placement area is provided with a fluid chip placement platform and a fixing part, the fluid chip placement platform is detachably arranged in the fixing part, and a plurality of fluid chips are arranged on the fluid chip placement platform.
The fluid chip pipetting device as described in at least one of claims 1 to 11 is characterized in that the fluid chip pipetting device is used for sequencing.
A method for pipetting a fluid chip, characterized in that it uses the fluid chip pipetting device according to any one of claims 1 to 11, and the pipetting method comprises:

The moving component drives the liquid transfer device to inject liquid into the fluid chip;

The moving component drives the liquid transfer device to aspirate liquid from the fluid chip;

The drying device blows air to dry the fluid chip.
The method for transferring liquid to a fluid chip according to claim 13, wherein the step in which the moving component drives the transfer device to transfer liquid to the fluid chip specifically comprises the following steps:

S11, the moving component drives the pipetting device to move to the pipette tip consumables area, and drives the pipetting device to descend to obtain the pipette tip;

S12, the moving component drives the liquid transfer device to move to the reagent storage area, and the liquid transfer device absorbs the reagent;

S13, the moving component drives the liquid transfer device to move to the fluid chip placement area, and the liquid transfer device injects liquid into the fluid chip;

S14, the moving component drives the pipetting device to move to the tip recovery area, and the pipetting device discards the tip.
The method for pipetting a fluid chip according to claim 13 or 14, characterized in that the step in which the moving component drives the pipetting device to aspirate the fluid chip specifically comprises the following steps:

S21, the moving component drives the pipetting device to move to the pipette tip consumables area, and the pipetting device descends to obtain the pipette tip;

S22, the moving component drives the liquid transfer device to move to the fluid chip placement area, and the liquid transfer device aspirates the fluid chip;

S23, the moving component drives the liquid transfer device to move to the tip recovery area, and the liquid transfer device discharges the waste liquid and discards the tip.
The method for transferring liquid using a fluid chip according to at least one of claims 13 to 15, wherein the step of blowing and drying the fluid chip by the drying device specifically comprises the following steps:

S31, the moving component drives the drying device to move above the chip port of the fluid chip;

S32, the moving device drives the drying device to move downward, so that the air outlet of the drying device is in contact with the chip port;

S33, the drying device blows air to dry the fluid chip;

S34, repeat steps S31 to S33 until the drying device completes the air blowing drying of all fluid chips.