WO2024076689A3 - Size-discriminating nucleic acid-binding complexes and methods of use thereof - Google Patents
Size-discriminating nucleic acid-binding complexes and methods of use thereof Download PDFInfo
- Publication number
- WO2024076689A3 WO2024076689A3 PCT/US2023/034568 US2023034568W WO2024076689A3 WO 2024076689 A3 WO2024076689 A3 WO 2024076689A3 US 2023034568 W US2023034568 W US 2023034568W WO 2024076689 A3 WO2024076689 A3 WO 2024076689A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- methods
- size
- binding complexes
- nucleic acids
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 238000007385 chemical modification Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Aspects of the present disclosure provide complexes configured to selectively bind nucleic acids of particular sizes, sequences, and chemical modifications. The disclosure further outlines methods for using these nucleic acid binding complexes to enrich specific nucleic acids from heterogeneous samples, and to optionally tag, sequence, or amplify the enriched nucleic acids.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202263413821P | 2022-10-06 | 2022-10-06 | |
US63/413,821 | 2022-10-06 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2024076689A2 WO2024076689A2 (en) | 2024-04-11 |
WO2024076689A3 true WO2024076689A3 (en) | 2024-06-20 |
Family
ID=90608955
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2023/034568 WO2024076689A2 (en) | 2022-10-06 | 2023-10-05 | Size-discriminating nucleic acid-binding complexes and methods of use thereof |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2024076689A2 (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20220290130A1 (en) * | 2021-03-11 | 2022-09-15 | Nautilus Biotechnology, Inc. | Systems and methods for biomolecule retention |
-
2023
- 2023-10-05 WO PCT/US2023/034568 patent/WO2024076689A2/en unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20220290130A1 (en) * | 2021-03-11 | 2022-09-15 | Nautilus Biotechnology, Inc. | Systems and methods for biomolecule retention |
Non-Patent Citations (1)
Title |
---|
BEAMISH ERIC, TABARD-COSSA VINCENT, GODIN MICHEL: "Digital counting of nucleic acid targets using solid-state nanopores", NANOSCALE, ROYAL SOCIETY OF CHEMISTRY, UNITED KINGDOM, vol. 12, no. 34, 4 September 2020 (2020-09-04), United Kingdom , pages 17833 - 17840, XP093184572, ISSN: 2040-3364, DOI: 10.1039/D0NR03878D * |
Also Published As
Publication number | Publication date |
---|---|
WO2024076689A2 (en) | 2024-04-11 |
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