WO2022082487A1

WO2022082487A1 - Anti-aging composite nutrient containing coenzymes and bioactive hydrogen, preparation method therefor and application thereof

Info

Publication number: WO2022082487A1
Application number: PCT/CN2020/122412
Authority: WO
Inventors: 朱上翔
Original assignee: 朱上翔
Priority date: 2020-10-21
Filing date: 2020-10-21
Publication date: 2022-04-28

Abstract

Disclosed is an anti-aging composite nutrient containing coenzymes and bioactive hydrogen. The nutrient contains bioactive hydrogen, coenzyme I, coenzyme II and adenosine triphosphate; the concentration of the biological hydrogen element of the bioactive hydrogen is 1000 ppb-1600 ppb; the total content of the coenzyme I, the coenzyme II and adenosine triphosphate is >36 ppm; and the pH of the composite nutrient=4-6. The preparation method therefor comprises: carrying out microecological, active bacterium, quasi-anaerobic and micro-fermentation biological reactions on a plant extraction solution, milk, egg liquid or a herbal traditional Chinese medicine plant extraction solution in the presence of a dehydrogenation catalyst ACT, so as to obtain an anti-aging composite nutrient containing coenzymes and bioactive hydrogen.

Description

An anti-aging compound nutrient containing coenzyme and biologically active hydrogen and its preparation method and application

technical field

The invention belongs to the field of food, and relates to the composite nutrient, in particular to an anti-aging composite nutrient containing coenzyme and biologically active hydrogen and a preparation method and application thereof.

Background technique

Coenzyme I, also known as mitochondrial hormone, common abbreviation: NAD+ (oxidized form), NADH (reduced form), chemical name: nicotinamide adenine dinucleotide or nicotinic acid diphosphate, English name: Nicotinamide adenine dinucleotide hydrate, molecular formula : C ₂₁ H ₂₇ N ₇ O ₁₄ P ₂ , molecular weight: 663.4.

Coenzyme I is an important coenzyme in human redox reactions and plays an important role in material metabolism and energy metabolism. At the same time, it is the only substrate of NAD+-dependent ribosyltransferase and has a series of biological functions. In our body, NAD+ is an active substance involved in thousands of important physiological reactions such as cell metabolism, redox, protein transcription, etc. It is a kind of vital substance. Also commonly referred to as the first human coenzyme.

In recent years, the foreign health industry has developed by leaps and bounds, and various new and effective coenzymes and active hydrogen products have been developed to delay aging and improve immunity. At present, only a few domestic enterprises are engaged in the production of health care products related to gene therapy. Most of the research and development work on coenzymes and active hydrogen is still carried out in the laboratory of the university. There are even fewer units developing new products for NADH and NMN.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is to provide a complex nutrient containing coenzyme and biologically active hydrogen for improving immunity, anti-oxidation and anti-aging.

In order to solve the above technical problems, the technical solution provided by the present invention is: an anti-aging composite nutrient containing coenzyme and biologically active hydrogen: containing biologically active hydrogen, coenzyme I, coenzyme II and adenosine triphosphate (ATP);

The biological hydrogen concentration of the biologically active hydrogen: 1000ppb～1600ppb;

The total content of described coenzyme I, coenzyme II and adenosine triphosphate is >36ppm;

The coenzyme I is NAD+/or NADH; the coenzyme II is NADP+ or NADPH.

The pH of the complex nutrients is 4-6.

The second technical problem to be solved by the present invention is to provide a method for preparing an anti-aging complex nutrient containing coenzyme and biologically active hydrogen.

In order to solve the second technical problem, the technical scheme adopted is: a preparation method of an anti-aging compound nutrient containing coenzyme and biologically active hydrogen, the steps of which include: plant extract, milk, egg liquid or herbal Chinese medicine plant extract as The substrate, in the presence of the dehydrogenation catalyst ACT, undergoes the biochemical reactions of microecology, active bacteria, quasi-anaerobic and micro-fermentation to obtain anti-aging complex nutrients containing coenzyme and biologically active hydrogen.

The dehydrogenation catalyst ACT accounts for 5% to 10% of the mass of the plant extract, milk, egg or herbal Chinese medicine plant extract.

Described a kind of preparation method of anti-aging compound nutrient containing coenzyme and biologically active hydrogen, its concrete steps include:

a) Plant extract, milk, egg liquid or herbal Chinese medicine plant extract as the substrate, adjust the initial temperature of the substrate to be 38 ° C ~ 43 ° C, keep the temperature, then adjust the pH of the substrate pH = 6 ~ 6.8, add dehydrogenation Catalyst ACT, stirring for 3-5min;

b) A certain amount of space is left above the liquid level, and then the reactor is sealed;

c) standing, through the biochemical reaction of microecology, active bacteria, quasi-anaerobic and micro-fermentation;

d) Reaction for 2.5h～24h, through the sensor outside the reaction kettle, start to detect various parameters in the reaction kettle: temperature, pressure, interface potential ORP potential or biohydrogen concentration, pH value;

When the biohydrogen concentration reaches or exceeds 1000ppb, the reaction is terminated;

e) Keep the reactor in a sealed state, and use pasteurization to sterilize the complex nutrients;

f) Keep the reactor in a sealed state, and cool down the complex nutrients to 4°C.

g) Under the conditions that meet the requirements of the production environment of medical and health products, the compound nutrients are sealed and packed in qualified containers or flexible packaging bags; the products are refrigerated in a 4°C environment.

In step a), after the dehydrogenation catalyst ACT is added, glucose with 1% to 5% of the total mass of the substrate is added as a carbon source.

The dehydrogenation catalyst ACT is an active probiotic.

The active probiotics are lactic acid and bifidobacteria.

In the pasteurization method, the temperature is 68-80 DEG C, and the time is 15-20 min.

The preparation method of the anti-aging composite nutrient containing coenzyme and biologically active hydrogen, the preparation step further comprises: concentrating the anti-aging composite nutrient solution containing coenzyme and biologically active hydrogen, and at the same time using on-line supplementary hydrogen active hydrogen, Increase the content of reduced coenzyme I and active hydrogen to obtain an anti-aging complex nutrient concentrate containing coenzyme and biologically active hydrogen. Taking the anti-aging compound nutrient concentrate directly can ensure that the human body obtains enough coenzymes, and it is also beneficial to obtain a higher absorption effect.

The method for preparing an anti-aging compound nutrient containing coenzyme and biologically active hydrogen, the preparation step further comprises: according to the characteristics that coenzyme I, coenzyme II and ATP have different proportions, using supercritical carbon dioxide extraction technology to extract coenzyme I respectively , coenzyme II and ATP three components, respectively obtain coenzyme I, coenzyme II and ATP solid powder substances with purity of 96-99wt.

The third technical problem to be solved by the present invention is to provide an application of an anti-aging complex nutrient containing coenzyme and biologically active hydrogen in food nutritional supplements, health care products and cosmetic products; The anti-aging compound nutrient concentrate is used as a food nutritional supplement; the solid powdery substances of coenzyme I, coenzyme II and ATP with a purity of 96-99Wt.% are further prepared into tablets, capsules or injections, as Anti-aging, life-enhancing health and beauty products.

In this application document, containing biologically active hydrogen, coenzyme I, coenzyme II and adenosine triphosphate (ATP) are called anti-aging complex nutrients, also known as "ta nuclear", "ta nuclear" is English: Turn Age Hydrogen Essence corresponds to the abbreviation TAHE Chinese transliteration.

The relationship and difference between NMN and NADH: 1) The full name of NMN is β-Nicotinamide Mononucleotide, and the Chinese name is β-Nicotinamide Mononucleotide. NMN enters the human body, and with the help of the enzyme NMNAT, consumes a certain amount of adenosine triphosphate (ATP) at the same time, and finally converts it into the anti-aging factor NAD+. NMN is only one of the 4 precursors of coenzyme I. 2) NADH is the reduced state of coenzyme I NAD+. NADH contains biologically active hydrogen. By entering the human body, NADH is directly decomposed into NAD+ and hydrogen (H), and at the same time releases a certain amount of adenosine triphosphate (ATP). 3) The difference between NMN and NADH: First, NMN needs the help of specific enzymes and consumes energy to synthesize NAD+; while NADH enters the body without the help of specific enzymes, it can directly decompose NAD+, and it also generates energy. One is synthesis and the other is decomposition, one is conditionally restricted, and the other is not so restricted. Therefore, relatively speaking, the conversion efficiency of NADH to NAD+ is much higher than that of NMN. Secondly, NMN needs to consume energy. We know that energy is extremely important for life activities, while NADH is the opposite. It can generate energy, which can be supplied to immune cells. Therefore, NADH is also very effective in enhancing immunity and helps the body to clear bacteria faster. Virus, strengthen the physique. 4) The NADH of "Tanuclide" will also decompose special biologically active hydrogen, which can enter the mitochondria inside the cell to fight free radicals and inflammation from the source, and has strong antioxidant properties. Ordinary hydrogen, if it is in a free state, cannot enter the interior of cells, but can only remove free radicals that are freed from cells. Antioxidative properties are limited.

Beneficial effect: Taking NADH directly is more efficient and effective than taking NMN. In addition to NAD+/NADH, "Tanuclide" also includes other three components generated by coenzyme I: NADP+/NADPH, ATP and biologically active hydrogen. Therefore, "Tanuclide" is a complex nutrient with anti-aging, anti-oxidant and direct energy for cells or specific organs. At the same time, the "tower nuclide" (weak acidity) generated by the "tower core" process and special packaging and preservation process (metal material cans or aluminum film flexible packaging, tight sealing, low temperature 4 degrees refrigeration), taking method (rice After taking it or taking alkaline hydrogen-rich water at the same time), it can overcome the obstacles of taking NADH directly: fear of high temperature decomposition, fear of gastric acid decomposition and instability. As for the molecular size, the relative molecular weights of NADH and NMN are only doubled, and there may be no essential difference when entering the cell membrane. The amount of nutrients contained in a unit of "tower nuclide" is far higher than that of NMN. "Tanuclide" complies with food safety requirements, as well as safety requirements for cosmetic facial masks.

In addition, in the preparation process of "ta nuclide", referred to as "ta nuclear" process, the substrates used are mainly natural green plants or Chinese herbal medicines, animal milk, poultry eggs, etc., without chemical additives. Sometimes, scraps from tea, agricultural and sideline processing, fruit processing, and discarded "wastes" can be used as substrates to process various advanced nutrient solutions, which can be used by humans, or livestock and poultry breeding industries to improve immunity and anti-inflammatory properties. Oxidative and anti-aging. In particular, if the traditional Chinese medicine (special medicine formula, ancestral formula, secret recipe, etc.) is selected as the substrate in the "tower core" process, the efficacy of the obtained Chinese medicine may be greatly improved. For example, in Example 4, using the anti-new crown virus Qingfei Decoction publicly recommended to the world by the National Health and Family Planning Commission as the substrate, and using the "Tahe" process, the obtained "Tahe" anti-new crown virus Qingfei Decoction was tested. It shows that "Tahe" anti-new coronavirus Qingfei Decoction has more abundant coenzyme and energy factors.

Description of drawings

Figure 1 is a flow chart of the preparation of "tower nuclide".

Figure 2 is the relationship between the total mitochondrial content and the substrate in "Tanuclide".

Among them, the data on the curve in Fig. 2 is the result of concentrating the measured data by 5 times.

Detailed ways

Example 1

To make Oxyhydrogen "Tower Core" Tea:

a) Prepare 7-10 grams of green tea. In order to prevent the possible existence of pesticide residues in tea leaves, the first step should be to remove pesticide residues: put the prepared normal temperature electrolyzed water (with a pH value of 11±0.5) into a container containing tea leaves, submerge the tea leaves, and steep them for 10-15 minutes, take out the cleaned tea leaves, put them in a casserole, add 780 ml of cold water, first boil with high heat, immediately change to low heat, keep boiling for 20 minutes, pour out the tea juice and store it in the container. Then pour 785 ml of cold water into the casserole, keep it boiling for 30 minutes in the same way, pour out the tea juice and store it in the above-mentioned container of about 750 ml, a total of about 1500 ml of tea, as the production of "Tanuclide" "substrate.

b) Place the substrate in the reactor. First measure and adjust the initial temperature of the tea water to be 38°C to 43°C and keep warm. Then adjust the pH of the substrate to pH=6～6.8. An ACT-dehydrogenation catalyst (which can be active probiotics such as lactic acid and bifidobacteria) with 5% to 10% of the total mass of the substrate is added. When necessary, 1% to 5% of the total mass of the substrate can be added with glucose as a carbon source. Stir for 3 to 5 minutes.

c) Note that there is a certain amount of space above the upper liquid level, and then tightly seal the reactor.

d) Let stand to start the reaction.

e) After 3-6 hours, through the detection outside the reactor, the sensor of the control unit starts to detect various parameters in the reactor: temperature, pressure, interface potential ORP potential or dissolved hydrogen concentration, pH value, etc.

When the main parameter reaches a predetermined value, for example, when the hydrogen concentration reaches or exceeds 1100 ppb, the reaction is terminated. The product in the reaction kettle is "tower core" tea. The hydrogen concentration of "Tower" tea can reach or exceed 1600ppb.

f) Keep the reaction kettle in a sealed state, and use pasteurization method (68～80℃, 15～20min) to sterilize the "Tahe" tea.

g) Keep the reactor in a sealed state, and let the "Tower Core" tea cool down to 4°C.

h) Open the reactor, and seal the "Tower Core" tea in a qualified container or flexible packaging bag under the conditions that meet the requirements of the production environment of medical and health products (GMP workshop). The product was then refrigerated in a 4°C environment. Deliver to markets or consumers with cold chain logistics.

Example 2

Make lactohydrogen "tower core" milk.

Similar to the steps of Example 1. The main difference is the use of fresh milk (goat milk, camel milk) or liquid milk prepared with milk powder or milk and soybean powder.

As a substrate for making "tower core" milk. Steps b) to h) are basically the same. However, the time for starting the detection of parameters in step e) is relatively short. After 2.5 ~ 3.5h, it is necessary to start paying attention, not too late. Otherwise, it will be over-fermented and it will be difficult to produce "tower core" milk. We noticed that the total amount of mitochondria contained in "Tanu" milk was as high as 372 mg/L. It is more than 2 times that of oxohydrogen "tower nuclide". It shows that "Tanu" milk has very rich anti-aging and immunity-enhancing functions. The hydrogen concentration of "tower core" milk can reach or exceed 1400ppb.

Example 3

Make 4 flavors of Immortal Grass "Tower Core" Health Essence.

Similar to the steps of Example 1. The main difference is the use of dendrobium, licorice, American ginseng and its balancer - 2 grams of astragalus, which are among the nine major immortal grasses in Chinese herbal medicine liquid, as the substrate for making the Immortal Grass "Tower Core" health essence. The remaining steps b) to h) are almost identical. We noticed that the total amount of mitochondria contained in the "ta nuclide" made with 3 to 4 herbs was as high as 410.3 mg/L. It is more than twice that of "Tahe" tea, and higher than that of "Tahe" milk. It is suitable for middle-aged and elderly people to supplement the needs of a large amount of coenzymes in the body due to aging. The hydrogen concentration of Immortal Grass "Tower Core" health essence can reach or exceed 1300ppb.

Example 4

Make anti-coronavirus "Tahe" Qingfei Decoction.

Similar steps to Example 3. The main difference is that the active liquid boiled out of 20 herbs in the secret recipe for Qingfei is used as the substrate for making "Tahe" Qingfei Decoction. The components of these 20 Chinese herbal medicines are: honeysuckle, platycodon, agarwood, agarwood, tangerine peel, cumin, yam, winter flower, aster, ginger, astragalus, Bupleurum, Poria, Atractylodes, Polyporus, Alisma, Guizhi, Gypsum, Licorice, Ephedra. The weight of each component is determined by the optimized formula of the secret recipe.

The production steps b) to h) are almost identical. We noticed that the total amount of mitochondria contained in "Tanuclide" made of 20 Chinese herbs was as high as 857.9 mg/L. It is a "tower nuclide" with the highest total amount of mitochondria obtained in the experiment process of the present invention. The hydrogen concentration of "Tahe" Qingfei Decoction can reach or exceed 1100ppb.

Example 5

Make a botanical "Tanuclide" beauty serum.

Similar steps to Example 1. The main difference is that the active liquid of several plant components is used as the substrate for making the plant "Tanuclide" beauty essence. The components of these plants are:

Fungus (10-15g), Tremella (10-15g), Fresh Taro (5-10g), Yam (5-10g), Skimmed Milk Powder (3-5g), Egg White Powder (3-5g) , Tower nuclear tea (1500-2000ml), drinking water (2500-3000ml).

The production steps b) to h) are almost identical. The measured results of the "Tanuclide" beauty essence are as follows: active hydrogen concentration C=1157ppb; pH=6.1; NAD+=1.2117mg/L; NADH=4.4902mg/L; NADP+=0.5549mg/L; NADPH=3.6942 mg/L; ATP=124.4157mg/L; converted into the total effective mitochondrial content of 135.47ppm. This product is composed of plant food, without chemical additives, rich in anti-aging compound nutrients and active hydrogen, and has the functions of anti-oxidation, nourishing skin cells, and anti-wrinkle. Use it as a mask or on your skin and face.

Figure 1 Analysis Description:

1) Fig. 1 is the preliminary result after data analysis and arrangement after the preparation method disclosed in the present application, 52 test samples were prepared, and the samples were subjected to the detection of coenzyme content (including coenzyme I, coenzyme II, ATP);

2) Since coenzyme II and ATP are products generated by coenzyme I as a substrate, in order to measure and compare the quality of the "tower nuclide" produced by different substrates, we divide the molar amounts of coenzyme II and ATP through biochemical reactions. The corresponding relationship is converted into an equivalent (or equivalent) coenzyme I (ie, mitochondrial hormone), and then added to the measured mitochondrial content to obtain the total mitochondrial content, which is used as a basis for comparison.

3) The following preliminary conclusions can be drawn from Fig. 2: (a) the content of mitochondrial hormone obtained with oxalohydrogen as substrate is not as high as that obtained with lactohydrogen and ovohydrogen as substrate; (b) the content of mitochondrial hormone obtained with green tea The mitochondrial content obtained as a substrate was higher than that obtained with Sydney pear and dragon fruit; (c) the content of mitochondrial obtained with ovohydrogen (protein albumin) as a substrate was higher than that obtained with oxalohydrogen and lactohydrogen. (d) The mitochondrial content produced by using multiple (3 or more) oxahydrogen-containing plants (oxahydrogen) as a substrate is much higher than that of a single oxahydrogen. This may be due to the synergistic effect of different functional herbs, which greatly improves the efficiency of producing mitochondrial content. The anti-COVID-19 Qingfei Decoction (containing 20 herbs) achieved surprising results with a content of 857.9 mg per liter. Nearly one gram of mitochondrial hormone per liter.

Using the preparation method disclosed in the present application, 52 "tower nuclides" (liquid samples) were prepared and obtained, which were detected and analyzed. After detection, it was indicated that a certain concentration of NAD+/NADH, NADP+/NADPH and ATP components. (The content of biohydrogen, including oxahydrogen, lacthydrogen and ovohydrogen, reaches an effective concentration of 1000ppb to 1600ppb). According to the definition of the total content of nutrients defined by us, the test results are: milk "tower nuclide" (C) = 134.06ppm; duck egg "tower nuclide" (C) = 209.31ppm; Sydney - pitaya "tower nuclide" ( C) = 47.00ppm; edamame "ta nuclide" (C) = 44.90ppm; soybean "ta nuclide" (C) = 36.56ppm; herbal beauty "ta nuclide" (C) = 135.47ppm; new coronavirus clearing the lungs Soup "Tower Nuclide" (C) = 272.21 ppm. Facts have proved that the concentration of the original solution of "tower nuclide" is quite high, and it has practical value.

Claims

An anti-aging complex nutrient containing coenzyme and bioactive hydrogen: containing bioactive hydrogen, coenzyme I, coenzyme II and adenosine triphosphate;

The biological hydrogen concentration of the biologically active hydrogen: 1000ppb～1600ppb;

The total content of described coenzyme I, coenzyme II and adenosine triphosphate is >36ppm;

The pH of the complex nutrients is 4-6.
The method for preparing an anti-aging compound nutrient containing coenzyme and biologically active hydrogen according to claim 1, characterized in that: plant extract, milk, egg liquid or herbal Chinese medicine plant extract are used as substrates, and the dehydrogenation catalyst ACT is used as a substrate. In the presence of micro-ecology, active bacteria, quasi-anaerobic and micro-fermentation biochemical reactions, anti-aging complex nutrients containing coenzyme and biologically active hydrogen are obtained.
The method for preparing an anti-aging compound nutrient containing coenzyme and biologically active hydrogen according to claim 2, wherein the dehydrogenation catalyst ACT is composed of plant extract, milk, egg liquid or herbal Chinese medicine plant extract 5% to 10% of the liquid quality.
The preparation method of a kind of anti-aging compound nutrient containing coenzyme and biologically active hydrogen according to claim 2 or 3, it is characterized in that: its concrete steps comprise:

a) Plant extract, milk, egg liquid or herbal Chinese medicine plant extract as the substrate, adjust the initial temperature of the substrate to be 38 ° C ~ 43 ° C, keep the temperature, then adjust the pH of the substrate pH = 6 ~ 6.8, add dehydrogenation Catalyst ACT, stirring for 3-5min;

b) A certain amount of space is left above the liquid level, and then the reactor is sealed;

c) standing, through the biochemical reaction of microecology, active bacteria, quasi-anaerobic and micro-fermentation;

d) Reaction for 2.5h～24h, through the sensor outside the reaction kettle, start to detect various parameters in the reaction kettle: temperature, pressure, interface potential ORP potential or biohydrogen concentration, pH value;

When the biohydrogen concentration reaches or exceeds 1000ppb, the reaction is terminated;

e) keep the reaction kettle in a sealed state, and use pasteurization to sterilize the complex nutrients; for the pasteurization, the temperature is 68-80°C, and the time is 15-20min;

f) Keep the reactor in a sealed state, and cool down the complex nutrients to 4°C.

g) Under the conditions that meet the requirements of the production environment of medical and health products, the compound nutrients are sealed and packed in qualified containers or flexible packaging bags; the products are refrigerated in a 4°C environment.
The method for preparing an anti-aging complex nutrient containing coenzyme and biologically active hydrogen according to claim 4, characterized in that: in step a), after adding a dehydrogenation catalyst ACT, adding 1% to 5% of the total mass of the substrate of glucose as a carbon source.
The method for preparing an anti-aging composite nutrient containing coenzyme and biologically active hydrogen according to claim 4, wherein the dehydrogenation catalyst ACT is an active probiotic.
The method for preparing an anti-aging compound nutrient containing coenzyme and biologically active hydrogen according to claim 6, wherein the active probiotics are lactic acid and bifidobacteria.
The method for preparing an anti-aging composite nutrient containing coenzyme and biologically active hydrogen according to claim 4, wherein the preparation step further comprises: concentrating the anti-aging composite nutrient solution containing coenzyme and biologically active hydrogen, At the same time, the active hydrogen of hydrogen is supplemented online to increase the content of reduced coenzyme I and active hydrogen to obtain an anti-aging complex nutrient concentrate containing coenzyme and biologically active hydrogen.
The method for preparing an anti-aging compound nutrient containing coenzyme and biologically active hydrogen according to claim 4, wherein the preparation step further comprises: using supercritical carbon dioxide extraction technology to extract coenzyme I, coenzyme II and ATP three respectively. Various components are obtained to obtain coenzyme I, coenzyme II and ATP solid powdery substances with a purity of 96-99wt.
Application of an anti-aging complex nutrient containing coenzyme and biologically active hydrogen according to any one of claims 1 to 9 in food nutritional supplements, health care products and cosmetic products.