WO2021026799A1

WO2021026799A1 - Embedding mold and method of using same

Info

Publication number: WO2021026799A1
Application number: PCT/CN2019/100520
Authority: WO
Inventors: 王璐璐; 鲁艺; 曹燚; 钟成; 潘苏婉; 王立平
Original assignee: 中国科学院深圳先进技术研究院
Priority date: 2019-08-14
Filing date: 2019-08-14
Publication date: 2021-02-18

Abstract

An embedding mold and a method of using same. The embedding mold comprises a mold main body (1) comprising an accommodation member (11) and a base (12). The accommodation member (11) has an accommodating hole (111) vertically extending therethrough. The base (12) matches the accommodating hole (111) and can detachably seal the accommodating hole (111). The embedding method employs the embedding mold to embed a tissue sample in an embedding medium. The accommodation member (11) and the base (12) of the embedding mold are components of fixed shapes. An accommodation cavity for accommodating a tissue sample and an embedding medium can be formed by pushing the base (12) into the accommodation member (11). After completion of a freezing operation, the embedded sample can be removed from the embedding mold by pushing the base (12) upwards.

Description

Embedding mold and its use method

Technical field

The invention relates to the technical field of biological tissue embedding, in particular to an embedding mold and a method of use thereof.

Background technique

Frozen sectioning is a commonly used method for obtaining tissue specimens in immunohistochemistry, and it is fast, convenient and precise. The process of preparing tissue specimens requires embedding the sample with an embedding agent and placing it in an environment of -80°C and freezing it to a certain degree of hardness, and then performing section processing to obtain specimen sections.

Since the embedding agent is in a liquid state when embedding the sample, a mold is required to hold the sample and the embedding agent when embedding the sample; in the prior art, the mold is generally made of tin foil, and the production steps are as follows: cut a piece Fold the tin foil and fold it into a mold with a cavity. Because the tin foil is prone to deformation, the process of manually folding the mold is difficult and low efficiency; after the embedded sample is frozen, unfold the tin foil and put it in a frozen state The embedded samples were taken out, and the tin foil was discarded.

That is, the operation process of embedding samples in the existing embedding mold is difficult and low in efficiency.

Summary of the invention

The purpose of the present invention is to provide an embedding mold and a method of use thereof, so as to solve the technical problems of high difficulty and low efficiency in the operation process of embedding samples of the embedding mold in the prior art.

The embedding mold provided by the present invention includes a mold body, the mold body includes a containing body and a base, the containing body is provided with a through containing hole along the vertical direction, and the base matches the containing hole for The receiving hole can be detachably blocked.

Further, the cross section of the receiving hole is circular.

Further, the receiving hole extends in a vertical direction, and the bottom surface of the receiving body is arranged parallel to the horizontal plane.

Further, a leg is provided on the outer wall of the containing body, the leg extends in a horizontal direction, and the bottom surface of the leg is coplanar with the bottom surface of the containing body.

Further, there are multiple mold bodies, and two adjacent mold bodies are connected by a connecting piece, and the bottom surfaces of the mold bodies are coplanar.

Further, the connecting piece extends in a horizontal direction, and the bottom surface of the connecting piece is coplanar with the bottom surface of the containing body.

Further, a plurality of the receiving bodies are arranged in an array.

Further, the containing body and the connecting piece are integrally formed.

Further, the mold body is formed by 3D printing.

Another object of the present invention is to provide an embedding method, using the embedding mold described above to embed a tissue sample with an embedding agent, and the operation steps include:

Push the base into the accommodating hole of the embedding mold and block the bottom of the accommodating hole;

Put the tissue sample into the containing hole through the top opening of the containing hole;

Pour the embedding agent into the containing hole through the top opening of the containing hole;

Freeze the embedding mold containing the tissue sample and embedding agent;

After the tissue sample is frozen, push the base toward the direction of the tissue sample until the base pushes the tissue sample embedded with the embedding agent out of the receiving hole;

Continue to push the base out of the receiving hole, or push the base to the initial blocking position of the receiving hole.

The beneficial effects of the embedding mold of the present invention are:

The embedding mold provided by the present invention includes a mold body capable of accommodating a tissue sample and an embedding agent to make it into a fixed shape, wherein the mold body includes a receiving body as a side panel and a base as a bottom plate. Removable connection between.

Before use, the accommodating body and the base are in a separated state, and the accommodating hole on the accommodating body is in a state where the accommodating body penetrates up and down. The base is pushed into it from one end of the accommodating hole. The outer side wall of the base is in contact with the inner side wall of the accommodating hole. The accommodating hole is blocked, and the base is pushed to the lower position of the accommodating hole to block it at the bottom of the accommodating hole. At this time, the top surface of the pedestal is set up, and the top surface of the base and the side wall of the accommodating hole together form an opening facing The accommodating cavity. Put the tissue sample into the containing cavity through the top opening, and then pour the embedding agent into the containing cavity. The embedding agent will flow to fill the gap between the tissue sample and the containing cavity and cover the top surface of the tissue sample; The mold body with the tissue sample and the embedding agent is frozen in a low temperature environment, and the tissue sample and the embedding agent are frozen into a solid embedding sample; when necessary, remove the mold body from the low temperature environment and push the base from bottom to top , The base moves upward in the containing hole, and pushes the solid embedded sample to move upward until the base pushes the entire embedded sample out of the containing hole, so that the embedded sample can be taken out of the mold body. After the embedded sample is taken out, it can be sliced; the base will continue to push up and push out the containing hole for the next embedding use, or push the base from top to bottom to the bottom blocking position of the containing hole to facilitate Use it directly next time.

The accommodating body and base of the embedding mold are parts with fixed shapes. Push the base into the accommodating body to form a accommodating cavity for tissue samples and embedding agent; after freezing, just push the base upwards to embed The sample is taken out from the embedding mold; that is, the base needs to be pushed during the entire operation, which is convenient and efficient. In addition, the containing body and the base can be recycled, which can also reduce the embedding cost and reduce the environmental pollution caused by waste.

Description of the drawings

In order to explain the specific embodiments of the present invention or the technical solutions in the prior art more clearly, the following will briefly introduce the drawings that need to be used in the specific embodiments or the description of the prior art. Obviously, the appendix in the following description The drawings are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work.

FIG. 1 is a schematic diagram of a three-dimensional structure in which a base in a mold body is inserted into a containing body according to an embodiment of the present invention;

2 is a schematic diagram of an exploded structure of a mold body provided by an embodiment of the present invention;

3 is a schematic diagram of a three-dimensional structure when legs are provided on the receiving body of the mold body according to an embodiment of the present invention;

4 is a schematic structural diagram of the mold body provided by an embodiment of the present invention connected together by a connecting body;

FIG. 5 is a schematic top view of the structure of the mold body connected together by the connecting body provided by the embodiment of the present invention.

Icon: 1-mold body; 11-receiving body; 111-receiving hole; 12-base; 2-leg; 3-connector.

detailed description

The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "up", "down", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate the orientation or The positional relationship is based on the position or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the pointed device or element must have a specific orientation, be constructed and operated in a specific orientation, Therefore, it cannot be understood as a limitation to the present invention.

In the description of the present invention, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense, unless otherwise clearly specified and limited. For example, they can be fixed or detachable. Connected or integrally connected; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal communication between two components. For those of ordinary skill in the art, the specific meaning of the above-mentioned terms in the present invention can be understood in specific situations.

This embodiment provides an embedding mold, as shown in Figs. 1 and 2, comprising a mold body 1. The mold body 1 includes a containing body 11 and a base 12. The containing body 11 is provided with a through containing hole 111 along the vertical direction, and The base 12 is matched with the receiving hole 111 and is used for detachably blocking the receiving hole 111.

The embedding mold provided in this embodiment includes a mold body 1 capable of accommodating a tissue sample and an embedding agent to make it into a fixed shape. The mold body 1 includes a receiving body 11 as a side panel and a base 12 as a bottom plate. The base 12 and the receiving body 11 are detachably connected.

Before use, the accommodating body 11 and the base 12 are in a separated state, and the accommodating hole 111 on the accommodating body 11 is in a state where the accommodating body 11 penetrates up and down. The base 12 is pushed into it from one end of the accommodating hole 111. The inner side walls contact and cooperate to seal the receiving hole 111, and push the base 12 to the lower position of the receiving hole 111 to block it at the bottom of the receiving hole 111. At this time, the top surface of the base 12 is set up, The top surface of 12 and the side wall of the receiving hole 111 jointly form a receiving cavity with an opening facing upward. The tissue sample is loaded into the containing cavity through the top opening, and then the embedding agent is poured into the containing cavity. The embedding agent flows to fill the gap between the tissue sample and the containing cavity and cover the top surface of the tissue sample; The mold body 1 with the tissue sample and the embedding agent is placed in a low temperature environment for freezing treatment, the tissue sample and the embedding agent are frozen into a solid embedding sample; if necessary, the mold body 1 is taken out from the low temperature environment, from bottom to top Push the base 12, the base 12 moves upward in the containing hole 111, and pushes the embedded sample in a solid state to move upward until the base 12 pushes the entire embedded sample out of the containing hole 111, so that the embedded sample is taken out of the mold body 1. After the embedded sample is taken out, it can be sliced; the base 12 will continue to push upward and push out the containing hole 111 for the next embedding use, or push the base 12 from top to bottom to the bottom of the containing hole 111 to block Location so that it can be used directly next time.

The accommodating body 11 and the base 12 of the embedding mold are parts with fixed shapes. Push the base 12 into the accommodating body 11 to form a cavity for accommodating the tissue sample and the embedding agent; after freezing, just push the base 12 upwards. , The embedded sample can be taken out of the embedding mold; that is, the base 12 needs to be pushed during the entire operation, which is convenient and efficient. In addition, the container 11 and the base 12 can be recycled, which can also reduce the embedding cost and reduce the environmental pollution caused by waste.

Specifically, for the above-mentioned embedding mold, this embodiment also provides an embedding method. The embedding mold is used to embed the tissue sample with the embedding agent. The operation steps include: pushing the base 12 into the embedding mold. In the containing hole 111, the base 12 blocks the bottom end of the containing hole 111; put the tissue sample into the containing hole 111 through the top opening of the containing hole 111; pour the embedding agent into the containing hole 111 through the top opening of the containing hole 111 ; Freeze the embedding mold containing the tissue sample and the embedding agent; after the tissue sample is frozen, push the base 12 toward the direction of the tissue sample until the base 12 pushes the tissue sample embedded with the embedding agent out of the containing hole 111; Continue to push the base 12 out of the receiving hole 111, or push the base 12 to the initial blocking position of the receiving hole 111.

Specifically, the above-mentioned low temperature environment may be a temperature environment close to -80°C; the top surface of the base 12 is coplanar with the horizontal plane to ensure that the bottom surface of the embedded sample is a flat surface; the embedding agent may be OCT (optimal cutting temperature compound, Optimum cutting temperature compound). OCT is a water-soluble mixture of polyethylene glycol and polyvinyl alcohol. It is a viscous liquid at room temperature. It will become a white hard solid after freezing. It is an ideal embedding agent.

In this embodiment, as shown in FIGS. 1 to 3, the cross section of the receiving hole 111 may be circular. When the cross section of the receiving hole 111 is circular, the side wall of the base 12 is correspondingly cylindrical. When the base 12 is inserted into the receiving hole 111, the base 12 can be inserted into the receiving hole 111 at any angle in the circumferential direction. When the cross section is rectangular or irregular, the base 12 must be rotated to a specific angle to insert. The circular receiving hole 111 is more convenient to operate; in addition, the cylindrical outer side wall of the base 12 and the cylindrical receiving hole 111 The inner side walls are matched with each other. When the base 12 is pushed up and down in the receiving hole 111, the interference between the base 12 and the receiving hole 111 is caused by interference and clamping, which greatly reduces the probability that the base 12 cannot be pushed, and further improves the operation of the embedding mold Convenience; in addition, the matching of the arc surface between the receiving hole 111 and the base 12 can also reduce the upward pushing process of the base 12, and the embedded sample is stuck at the corners and the embedded sample is damaged.

Specifically, as shown in Figures 1 to 3, the cross section of the outer side wall of the containing body 11 can also be circular, and the containing body 11 is cylindrical, which is more convenient to process, and the cost of the corresponding embedding mold can be reduced. Specifically, the height of the cylindrical containing body 11 can be 20mm, the diameter of the containing hole 111 can be 18mm, and the wall thickness can be 2mm; the base 12 can be a cylindrical plate with a diameter of 18mm and a thickness of 2mm. It should be noted here that, in addition to the above-mentioned circular shape, the cross section of the receiving hole 111 may also be rectangular, triangular or other irregular shapes, and the shape of the base 12 and the receiving hole 111 can be matched.

In this embodiment, as shown in FIGS. 1 to 3, the receiving hole 111 may extend in a vertical direction, and the bottom surface of the receiving body 11 is arranged parallel to the horizontal plane. Here is a specific form of the containing body 11. When in use, the base 12 is embedded in the containing hole 111, and the bottom surface of the base 12 does not protrude from the bottom surface of the containing body 11. When the containing body 11 is placed on a horizontal platform, the containing body 11 The bottom surface of the container 11 can be in contact with the water platform, and the contact surface is the entire bottom surface of the container 11, thereby improving the stability of the container 11 when placed, and reducing the occurrence of the outflow of the embedding agent and tissue samples caused by the dumping of the container 11 during the embedding process; In addition, when the accommodating body 11 is placed on the horizontal platform, the accommodating hole 111 is in a vertical cylindrical shape. When the tissue sample and the embedding agent are placed in the accommodating hole 111, the center of gravity of the tissue sample and the embedding agent is located in the accommodating hole 111. And it is located above the area where the bottom surface of the containing body 11 is located, thereby further reducing the occurrence of dumping of the embedded mold during the embedding process.

In this embodiment, as shown in FIG. 3, a leg 2 may be provided on the outer wall of the containing body 11, the leg 2 extends in a horizontal direction, and the bottom surface of the leg 2 and the bottom surface of the containing body 11 are coplanar. When the mold body 1 is placed on a water platform, the bottom surface of the container 11 and the bottom surface of the leg 2 are in contact with the water platform at the same time. The contact surface is the sum of the bottom surface of the container 11 and the bottom surface of the leg 2, and the contact area is enlarged and stable Correspondingly improved performance, further reducing the occurrence of the mold body 1 tipping; in addition, the legs 2 extend in the horizontal direction, which greatly increases the difficulty of the receiving body 11 tilting toward the direction of the legs 2, thereby further improving the stability of the mold body 1. Sex.

Specifically, the legs 2 may be multiple, and the multiple legs 2 are evenly arranged along the circumferential direction of the containing body 11, so that the multiple containing bodies 11 support various directions in the circumferential direction, and the difficulty of tilting the containing body 11 in all directions is uniform. Larger, thereby further improving the stability of the mold body 1. As shown in FIG. 3, there are four legs 2, and the four legs 2 are evenly arranged along the circumference of the containing body 11.

In this embodiment, as shown in FIG. 4 and FIG. 5, there may be multiple mold bodies 1, and two adjacent mold bodies 1 are connected by a connecting piece 3, and the bottom surfaces of the mold bodies 1 are coplanar. When a large number of the same tissue samples are required, the connecting piece 3 can be used to connect multiple mold bodies 1 into one body. The operation can be carried out in batches, such as placing the tissue samples in the receiving holes 111 of the multiple mold bodies 1, and then The embedding agent is injected into the containing holes 111 of the multiple mold bodies 1. After the operation is completed, the multiple mold bodies 1 formed as a whole can be placed in a low temperature environment for freezing treatment, and then taken out at the same time. Compared with the need of a single sample body Place and take out one by one, the operation is more convenient.

In this embodiment, as shown in FIGS. 4 and 5, the connecting member 3 may extend in a horizontal direction, and the bottom surface of the connecting member 3 and the bottom of the containing body 11 are coplanar. When a plurality of mold bodies 1 connected as a whole by a connector 3 are placed on a water platform, the bottom surfaces of the multiple mold bodies 1 and the bottom surfaces of the multiple connectors 3 are in contact with the water platform, and the contact area is larger and the stability is stronger In addition, in addition to the connecting piece 3 serving as the leg 2 to support the mold body 1, different mold bodies 1 can also play the role of the legs 2 mutually, which further improves the stability of the mold body 1 during use and reduces the occurrence of Inclined situation.

Specifically, in this embodiment, as shown in FIG. 5, a plurality of receiving bodies 11 are arranged in an array. Here is a specific arrangement of a plurality of containing bodies 11, a plurality of mold bodies 1 are connected into an integral form by a connector 3, and the plurality of mold bodies 1 are arranged in an array, and the overall shape is rectangular or ring-shaped, with regular shape and appearance The size is small, and when the space is small, it can accommodate a large number of mold bodies 1, so that on the basis of realizing the simultaneous use of multiple mold bodies 1, the space utilization is effectively improved; in addition, multiple mold bodies 1 are arranged It has a rectangular or circular shape, which is more stable than an irregular shape or a long strip. Specifically, there may be 16 mold bodies 1, and the 16 mold bodies 1 are arranged in a 4×4 array.

In this embodiment, as shown in FIG. 5, a plurality of accommodating bodies 11 may be arranged in a matrix array, and the connecting members 3 in the same row are arranged collinearly, and the straight line of the connecting members 3 passes through the axis centers of the accommodating holes 111 in the same row. And/or, the connecting pieces 3 located in the same row are arranged collinearly, and the straight line where the connecting pieces 3 are located passes through the axis of the receiving holes 111 in the same row. Specifically, the length of the connecting member 3 may be 10 mm, the width may be 5 mm, and the height may be 2 mm.

In this embodiment, the accommodating body 11 and the connecting member 3 may be integrally formed. The accommodating body 11 and the connecting piece 3 are integrally formed during processing, and the connection strength between the two is higher, and the bending or breakage during use is reduced correspondingly, and the practicability of the mold body 1 is further improved.

Specifically, the mold body 1 can be formed by 3D printing. A 3D printer is used to integrally print and form the receiving body 11 and the connecting member 3, and the base 12 can be printed out. Specifically, it takes 5 to 10 hours to print the 16 mold bodies 1 described above. Among them, the printing material can be ABS (acrylonitrile-butadiene-styrene copolyme, acrylonitrile-butadiene-styrene copolymer) or other printing materials.

It should be noted here that in addition to 3D printing, the base 12, the integrally formed container 11 and the connector 3 can be manufactured by other methods, such as injection molding, cutting processing, etc., all of which belong to the scope of protection of the present application.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: The technical solutions described in the foregoing embodiments are modified, or some or all of the technical features are equivalently replaced; these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

An embedding mold, characterized by comprising a mold body (1), the mold body (1) includes a containing body (11) and a base (12), the containing body (11) is provided with a through An accommodating hole (111), and the base (12) is matched with the accommodating hole (111), and is used to detachably block the accommodating hole (111).
The embedding mold according to claim 1, wherein the cross section of the receiving hole (111) is circular.
The embedding mold according to claim 1, wherein the containing hole (111) extends in a vertical direction, and the bottom surface of the containing body (11) is arranged parallel to the horizontal plane.
The embedding mold according to claim 3, wherein a leg (2) is provided on the outer wall of the containing body (11), the leg (2) extends in a horizontal direction, and the leg The bottom surface of (2) is coplanar with the bottom surface of the containing body (11).
The embedding mold according to any one of claims 1-3, characterized in that there are multiple mold bodies (1), and a connecting piece (3) is passed between two adjacent mold bodies (1). ) Are connected, and the bottom surfaces of the mold bodies (1) are coplanar.
The embedding mold according to claim 5, characterized in that the connecting piece (3) extends in a horizontal direction, and the bottom surface of the connecting piece (3) is coplanar with the bottom of the containing body (11).
The embedding mold according to claim 5, wherein a plurality of the containing bodies (11) are arranged in an array.
The embedding mold according to claim 5, characterized in that the containing body (11) and the connecting member (3) are integrally formed.
The embedding mold according to any one of claims 1-3, wherein the mold body (1) is formed by 3D printing.
An embedding method, characterized in that the embedding mold of any one of claims 1-9 is used to embed a tissue sample with an embedding agent, and the operation steps include:

Push the base (12) into the receiving hole (111) of the embedding mold correspondingly, and the base (12) blocks the bottom end of the receiving hole (111);

Put the tissue sample into the containing hole (111) through the top opening of the containing hole (111);

Pour the embedding agent into the containing hole (111) through the top opening of the containing hole (111);

Freeze the embedding mold containing the tissue sample and embedding agent;

After the tissue sample is frozen, push the base (12) toward the direction of the tissue sample until the base (12) pushes the tissue sample embedded with the embedding agent out of the containing hole (111);

Continue to push the base (12) out of the receiving hole (111), or push the base (12) to the initial blocking position of the receiving hole (111).