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WO2020196724A1 - Composition for transplanting bacterial flora, production method thereof, transplantation instrument, and transplantation method - Google Patents

Composition for transplanting bacterial flora, production method thereof, transplantation instrument, and transplantation method Download PDF

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Publication number
WO2020196724A1
WO2020196724A1 PCT/JP2020/013601 JP2020013601W WO2020196724A1 WO 2020196724 A1 WO2020196724 A1 WO 2020196724A1 JP 2020013601 W JP2020013601 W JP 2020013601W WO 2020196724 A1 WO2020196724 A1 WO 2020196724A1
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Prior art keywords
bacterial flora
composition
transplanting
transplantation
flora
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PCT/JP2020/013601
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French (fr)
Japanese (ja)
Inventor
永順 成
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株式会社晩聲社
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Priority to JP2020540827A priority Critical patent/JPWO2020196724A1/en
Publication of WO2020196724A1 publication Critical patent/WO2020196724A1/en
Priority to JP2021176517A priority patent/JP2022009670A/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system

Definitions

  • the present invention relates to a composition for transplanting a bacterial flora to an animal such as a pet, a method for producing the same, a device for transplantation, and a method for transplantation.
  • intestinal flora A wide variety of bacteria are clustered in the intestine, and the colony is called the intestinal flora (intestinal flora), which is a beneficial bacterium that prevents the invasion and growth of malicious bacteria and promotes intestinal motility. It contains bacteria that have a bad effect on the body, such as promoting food decay in the intestines and producing toxic substances, and bacteria that have a harmful effect when the pet's physical condition is sick.
  • This intestinal flora varies from animal to animal, and also changes depending on the dietary content, lifestyle and age, stress, lack of exercise, and so on.
  • Non-Patent Document 1 In the intestines of healthy pets, the intestinal flora is maintained in a certain balance by suppressing harmful bacteria by beneficial bacteria, but when harmful bacteria become predominant due to dietary habits and stress, the intestines As decay progresses, substances harmful to health increase. It is said that these harmful substances cause diarrhea and constipation, as well as allergies, skin diseases and obesity. For these reasons, attention has been focused on adjusting the intestinal flora not only for humans but also for pets such as dogs and cats in order to maintain their health (see, for example, Non-Patent Document 1).
  • Non-Patent Document 1 it has been found that the type and condition of the intestinal flora have characteristics and tendencies depending on the animal species according to their age and eating habits. The research results and know-how of the flora transplantation method cannot be applied as they are.
  • an object of the present invention is to provide a composition for transplanting a bacterial flora suitable for animals such as pets, a method for producing the same, a device for transplantation, and a method for transplantation. ..
  • the present invention presents a filtration step of filtering feces collected from animals and an extraction step of extracting the bacterial flora by centrifugation from the fecal bacterial flora material obtained by filtering in the filtration step. And a coloring step of blending a pigment material into the bacterial flora extracted in the extraction step.
  • the present invention is a composition for transplanting a bacterial flora produced by the above production method.
  • another invention is a transplantation device used for transplanting a bacterial flora transplantation composition produced by the above-mentioned production method, and is a storage unit having a compressible shape for accommodating the bacterial flora transplantation composition.
  • a tubular injection part connected to the containment part, and the length of the injection part inserted into the body of the animal to be transplanted in the injection direction is formed longer than the length of the containment part in the same direction. It is characterized by being.
  • the present invention has a filtration step of filtering feces collected from animals, an extraction step of extracting the bacterial flora by centrifugation from the fecal bacterial flora material obtained by filtering in the filtration step, and an extraction step.
  • For transplantation which comprises a coloring step of blending a pigment material into the prepared bacterial flora to produce a composition for bacterial flora transplantation, and a container having a compressible shape for the composition for bacterial flora transplantation formulated in the coloring step.
  • Bacteria comprising a containment step of accommodating in an instrument and an administration step of administering the composition for bacterial flora transplantation in the containment portion through a tubular injection portion connected to the accommodating portion by compressing the accommodating portion. This is a method for transplanting a composition for flora transplantation.
  • the method for transplanting the composition for transplanting the bacterial flora further includes a cryopreservation step of freezing and storing the stool bacterial flora material obtained in the filtration step prior to the administration step, and the extraction step includes frozen stool. It is preferable to thaw the flora material and extract the flora from the thawed fecal flora material.
  • the method for transplanting the composition for transplanting the bacterial flora further includes a cryopreservation step of freezing and storing the bacterial flora extracted in the extraction step prior to the administration step, and the coloring step includes frozen bacteria.
  • the flora may be thawed to produce a composition for bacterial flora transplantation from the thawed flora.
  • the method for transplanting the composition for transplanting the bacterial flora further includes a freezing storage step of freezing and storing the composition for transplanting the bacterial flora produced in the coloring step prior to the administration step, and the cryopreservation step further includes. , It may be stored in the storage portion used in the storage step, frozen and stored, and the storage step may be omitted.
  • the freeze-drying method is used for freezing in the above-mentioned freezing storage method, and the material frozen by this freeze-drying method can be thawed by bringing hydrogen water into contact with the material.
  • the bacterial flora is extracted by centrifugation from the fecal bacterial flora material obtained by filtering feces collected from animals, and a pigment material is applied to the extracted bacterial flora. Since it is blended, only an appropriate bacterial flora is extracted and colored with a pigment, so that an intestinal flora transplantation method suitable for the constitution of the pet can be carried out while reducing the feeling of resistance to handle feces.
  • FIG. 1 is a conceptual diagram showing an outline of the intestinal flora transplantation method according to the present embodiment.
  • the processing procedure described below is only an example, and each processing may be changed as much as possible. Further, with respect to the processing procedure described below, steps can be omitted, replaced and added as appropriate according to the embodiment.
  • a filtration step S01 for filtering feces collected from the donor animal A1 is performed.
  • 100 grams of feces provided by the donor animal A1 is turbid in the solution, and the turbid solution 2 is filtered 5 times through the sterile gauze 20 placed in the funnel 21.
  • This solution is produced by mixing 200 grams of saline with 100 grams of nanobubble hydrogen water.
  • This nanobubble hydrogen water is a solution produced by AC electrolysis and contains bubbles having a size of 1 / 1,000,000 mm.
  • an extraction step S02 is performed to extract the bacterial flora 42 by centrifugation from the fecal bacterial flora material 3 obtained by filtering in the filtration step S01.
  • a test tube containing the fecal flora material 3 is placed in the rotating body 40 of the centrifuge 4 and rotated to separate the precipitate 42b and the supernatant 42a, and unnecessary ones are separated. Remove.
  • the rotation speed of the centrifuge 1500 / sec is continued for 5 minutes to remove finer particles.
  • a coloring step S03 is performed in which the dye material 6 is blended with the bacterial flora 42c extracted by removing unnecessary substances in this way.
  • the pigment material 6 8.4% of edible yellow No. 4 and 3.6% of edible blue No. 1 are blended and colored green.
  • the pigment material to be blended in the bacterial flora in this coloring step S03 for example, a material extracted from the fruit of gardenia can be used.
  • Fruits of the genus Gardenia of the family Rubiaceae have been used as raw materials for crude drugs and Chinese herbs since ancient times, and they are harmless even if they enter the intestines.
  • Crocin which is a kind of carotenoid contained in fruits, also serves as a yellow coloring agent.
  • genipin contained in the gardenia fruit extract and amino acids contained in rice bran are fermented to produce a blue colorant, and 75% of the above yellow pigment and 20% of the blue pigment are mixed to make green. Color and mix 5% dextrin into powder.
  • the powder may be dissolved in water and the solution of the powder may be used for the final round of filtration of the flora.
  • a storage step S04 is performed in which the bacterial flora transplantation composition 5 blended in the coloring step S03 is stored in the storage portion 7a of the transplantation device 7.
  • the transplantation device 7 used in the storage step S04 is a device used for transplanting the above-mentioned bacterial flora transplantation composition 5, and contains the bacterial flora transplantation composition 5 and has a compressible shape. It includes a portion 7a and a tubular injection portion 7b connected to the accommodating portion 7a and communicated with the inside thereof.
  • the transplanting device 7 is formed so that the length of the injection portion 7b inserted into the body of the animal A2 to be transplanted in the injection direction is longer than the length of the accommodating portion 7a in the same direction.
  • the accommodating portion 7a of the transplanting instrument 7 is formed of a transparent material so that the contents can be clearly seen. Further, in the transplanting instrument 7 according to the present embodiment, the length of the accommodating portion 7a is 5 cm, and the length of the tubular injection portion 7b is formed to be about 10 cm. Then, as shown enlarged in FIG. 2, a memory 7d is attached in units of 1 cm so that the length of the injection portion 7b can be adjusted according to the type of the animal A2, and along the longitudinal direction of the injection portion 7b. A movable stopper 7c is attached so that the substantially length of the injection portion 7b inserted into the body of the animal A2 can be changed.
  • the administration step S05 for administering the composition 5 for microbiota transplantation to the animal A2 to be transplanted is performed.
  • the injection portion 7b is inserted into the anus of the animal A2, and the composition 5 for bacterial flora transplantation in the containment portion 7a is administered through the injection portion 7b by compressing the accommodation portion 7a.
  • the bacterial flora is extracted by centrifugation from the fecal bacterial flora material obtained by filtering feces collected from animals to obtain the extracted bacterial flora. Since the pigment material is blended, it is possible to carry out an intestinal flora transplantation method suitable for the constitution of the pet while extracting only the appropriate bacterial flora and reducing the psychological resistance to handle feces by coloring with the pigment. ..
  • FIG. 3 is a conceptual diagram showing an outline of the intestinal flora transplantation method according to the present embodiment. It should be noted that the embodiments shown below exemplify an apparatus or the like for embodying the technical idea of the present invention, and the technical idea of the present invention describes the material, shape, structure, and the material of each component. The arrangement etc. is not specified as the following. The technical idea of the present invention can be modified in various ways within the scope of claims.
  • the filtration step S01 for filtering the feces collected from the donor animal A1 is performed.
  • 100 grams of feces provided by the donor animal A1 is turbid in the solution, and the turbid solution 2 is filtered 5 times through the sterile gauze 20 placed in the funnel 21.
  • This solution is produced by mixing 200 grams of saline with 100 grams of nanobubble hydrogen water.
  • This nanobubble hydrogen water is a solution produced by AC electrolysis and contains bubbles having a size of 1 / 1,000,000 mm.
  • an extraction step S02 is performed to extract the bacterial flora 42 by centrifugation from the fecal bacterial flora material 3 obtained by filtering in the filtration step S01.
  • a test tube containing the fecal flora material 3 is placed in the rotating body 40 of the centrifuge 4 and rotated to separate the precipitate 42b and the supernatant 42a, and unnecessary ones are separated. Remove.
  • the rotation speed of the centrifuge 1500 / sec is continued for 5 minutes to remove finer particles.
  • a coloring step S03 is performed in which the dye material 6 is blended with the bacterial flora 42c extracted by removing unnecessary substances in this way.
  • the pigment material 6 8.4% of edible yellow No. 4 and 3.6% of edible blue No. 1 are blended and colored green.
  • the pigment material to be blended in the bacterial flora in this coloring step S03 for example, a material extracted from the fruit of gardenia can be used.
  • Fruits of the genus Gardenia of the family Rubiaceae have been used as raw materials for crude drugs and Chinese herbs since ancient times, and they are harmless even if they enter the intestines.
  • Crocin which is a kind of carotenoid contained in fruits, also serves as a yellow coloring agent.
  • genipin contained in the gardenia fruit extract and amino acids contained in rice bran are fermented to produce a blue colorant, and 75% of the above yellow pigment and 20% of the blue pigment are mixed to produce green. And mix 5% of dextrin into powder.
  • the powder may be dissolved in water and the solution of the powder may be used for the final round of filtration of the flora.
  • a freezing storage step S61 is performed in which the composition for bacterial flora transplantation produced in the coloring step S03 is frozen and stored.
  • freezing and storage are performed in a state of being contained in the composition for transplanting the bacterial flora in the storage portion 8a of the storage container 8 used in the storage step S04.
  • a freeze-drying method is used for freezing in this freezing storage step S61. Specifically, freeze-drying treatment is performed so that 100 cc of the composition for transplanting the bacterial flora (intestinal flora solution) is reduced by 30% in 8 hours, that is, 70 cc. Then, the frozen bacterial flora transplantation composition (intestinal flora solution) is collected and analyzed in step S62. As a result of this collection and analysis, it becomes possible to select an appropriate composition for bacterial flora transplantation (intestinal flora solution) according to the type and symptom of the animal A2 to be administered.
  • the microbiota transplantation composition (intestinal flora solution) collected here is not limited to those of animals of the same species, for example, microbiota transplantation compositions of other small animals having a disease or disease, pets.
  • the intestinal flora of a person whose living environment is similar, such as the owner of the animal, may be included, and a flora containing a large amount of bacterial flora specialized for a specific medical condition may be selected and used for treatment.
  • the composition for transplanting the bacterial flora may be frozen using liquid nitrogen and stored for a long period of time.
  • the intestinal flora when the animal A2 to be administered is young or in a healthy state is frozen and stored by freeze-drying or liquid nitrogen, and frozen when it becomes old or sick.
  • the intestinal flora that has been present may be thawed and administered.
  • the bacterial flora transplantation composition 5 formulated in the coloring step S03 is housed in the storage part 8a of the transplantation device 8 and frozen and stored S61.
  • the transplanting instrument 8 is an instrument used for transplanting the above-mentioned bacterial flora transplanting composition 5, and is an accommodating portion 8a that accommodates the bacterial flora transplanting composition 5 and has a compressible shape, and an accommodating portion. It is provided with a tubular injection portion 8b connected to 8a and communicated with the inside thereof.
  • the transplanting device 8 is formed so that the length of the injection portion 8b inserted into the body of the animal A2 to be transplanted in the injection direction is longer than the length of the accommodating portion 8a in the same direction.
  • the accommodating portion 8a of the transplanting instrument 8 is formed of a transparent material so that the contents can be clearly seen, and is formed in a bellows shape so as to be expandable and contractible in the axial direction.
  • the length of the accommodating portion 8a is 5 cm
  • the length of the tubular injection portion 8b is formed to be about 10 cm.
  • a scale 8d is provided in units of 1 cm so that the length of the injection portion 8b can be adjusted according to the type of the animal A2, and along the longitudinal direction of the injection portion 8b.
  • a movable stopper 8c is attached so that the substantially length of the injection portion 8b inserted into the body of the animal A2 can be changed.
  • a cap 83 is communicated with the rear end portion of the injection portion 8b so that it can be screwed into the accommodating portion 8a and connected.
  • the accommodating portion 8a can also be screwed into a cap 8c for closing separately, and by using the cap 8c for closing, the injection portion 8b is removed and suitable for freezing storage.
  • the thawing step S63 is performed.
  • the thawing step S63 it is preferable to thawing gently at, for example, human skin temperature (around 36 ° C.) or room temperature.
  • the frozen material may be thawed by bringing hydrogen water into contact with the frozen material.
  • the administration step S05 for administering the composition 5 for microbiota transplantation to the animal A2 to be transplanted is performed.
  • the injection portion 7b is inserted into the anus of the animal A2, and the composition 5 for bacterial flora transplantation in the containment portion 7a is administered through the injection portion 7b by compressing the accommodation portion 7a.
  • the composition for transplanting the bacterial flora (intestinal flora solution) produced in the coloring step S03 is frozen and stored, but the fecal flora material 3 obtained in the filtering step S01 is frozen.
  • the frozen fecal flora material may be thawed and the flora may be extracted from the thawed fecal flora material.
  • the bacterial flora extracted in the extraction step S02 is frozen and stored, and in the coloring step S03, the frozen bacterial flora is thawed and a composition for transplanting the bacterial flora is produced from the thawed bacterial flora. You may do so.
  • the bacterial flora is extracted by centrifugation from the fecal bacterial flora material obtained by filtering feces collected from animals to obtain the extracted bacterial flora. Since the pigment material is blended, it is possible to carry out an intestinal flora transplantation method suitable for the constitution of the pet while extracting only the appropriate bacterial flora and reducing the psychological resistance to handle feces by coloring with the pigment. ..
  • a donor bank-like service capable of appropriately selecting and providing an intestinal flora specialized for a specific disease or disease.
  • a part of the collected intestinal flora can be stored frozen first, and biological sample analysis can be performed at a specialized institution at intervals of 2 months.
  • Safety can be improved by thawing and using the flora that was first collected and stored frozen after the safety was confirmed.
  • the present invention is not limited to the above-described embodiments as they are, and at the implementation stage, the components can be modified and embodied within a range that does not deviate from the gist thereof.
  • various inventions can be formed by an appropriate combination of the plurality of components disclosed in the above-described embodiment. For example, some components may be removed from all the components shown in the embodiments.

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Abstract

[Problem] Provided are: a composition for transplanting bacterial flora suitable for an animal such as a pet; a production method thereof; a transplantation instrument; and a transplantation method. [Solution] A transplantation method according to the present invention comprises: a filtration step S01 for filtering a turbid liquid 2 including feces obtained from an animal A1; an extraction step S02 for extracting bacterial flora 42 from feces bacterial flora materials by means of centrifugation; a coloring step S03 for adding a dye material 6 to the bacterial flora 42 to produce a composition 5 for transplanting bacterial flora; a storage step S04 for storing, in a transplantation instrument 7, the composition 5 for transplanting bacterial flora; and an injection step S05 for injecting the composition 5 for transplanting bacterial flora through the transplantation instrument 7. The transplantation instrument 7 used herein is provided with: a storage part 7a having a shape in which the composition 5 for transplanting bacterial flora can be stored and compressed; and a tubular injection part 7b connected to the storage part 7a.

Description

細菌叢移植用組成物、その製造方法、移植用器具及び移植方法Bacterial flora transplantation composition, its production method, transplantation equipment and transplantation method
　本発明は、ペット等の動物に対する細菌叢移植用組成物、その製造方法、移植用器具及び移植方法に関する。 The present invention relates to a composition for transplanting a bacterial flora to an animal such as a pet, a method for producing the same, a device for transplantation, and a method for transplantation.
　近年、イヌや猫等のペットの食生活についても、人間と同様に腸内フローラの存在が注目されており、ペットについても腸内フローラを整えることで健康につなげることが研究され始めている。腸内には多種多様な細菌が群生しており、その群生は腸内フローラ（腸内細菌叢）と呼ばれ、悪質な菌の侵入や増殖を防いだり腸の運動を促す有益な菌や、腸内で食物の腐敗を進め、有毒物質をつくるなど、体に悪い働きをする菌、ペットの体調が崩れた時に有害な働きをする菌などがふくまれている。この腸内フローラには、動物の個体ごとにことなり、さらにはそのときどきの食事内容、生活習慣や年齢、ストレス、運動不足などによっても変化する。 In recent years, the existence of intestinal flora has been attracting attention in the dietary habits of pets such as dogs and cats, as in humans, and research has begun on pets that can lead to health by adjusting the intestinal flora. A wide variety of bacteria are clustered in the intestine, and the colony is called the intestinal flora (intestinal flora), which is a beneficial bacterium that prevents the invasion and growth of malicious bacteria and promotes intestinal motility. It contains bacteria that have a bad effect on the body, such as promoting food decay in the intestines and producing toxic substances, and bacteria that have a harmful effect when the pet's physical condition is sick. This intestinal flora varies from animal to animal, and also changes depending on the dietary content, lifestyle and age, stress, lack of exercise, and so on.
　健康なペットの腸内では、有益な菌が有害な菌を抑える形で腸内フローラが一定のバランスで維持されているが、食生活やストレスなどの原因で有害な菌が優勢になると腸内腐敗が進み健康に有害な物質が増加する。これらの有害物質が下痢や便秘等の原因になったり、アレルギー、皮膚疾患や肥満の原因になるとも言われている。こうしたことから、人間だけでなくイヌや猫等のペットでも健康維持のために、腸内フローラを整えることに注目が集まっている（例えば、非特許文献１参照）。 In the intestines of healthy pets, the intestinal flora is maintained in a certain balance by suppressing harmful bacteria by beneficial bacteria, but when harmful bacteria become predominant due to dietary habits and stress, the intestines As decay progresses, substances harmful to health increase. It is said that these harmful substances cause diarrhea and constipation, as well as allergies, skin diseases and obesity. For these reasons, attention has been focused on adjusting the intestinal flora not only for humans but also for pets such as dogs and cats in order to maintain their health (see, for example, Non-Patent Document 1).
　人間に対する研究では、腸内細菌やこれらの代謝物が、腸管の炎症抑制や免疫調整に重要な役割を果たしていることは広く知られているところであるが、近年、便微生物叢移植法(fecal microbiota transplantation: FMT)の可能性が示唆されている。 In human studies, it is widely known that intestinal bacteria and their metabolites play important roles in intestinal inflammation suppression and immune regulation, but in recent years, fecal microbiota transplantation method (fecal microbiota) The possibility of transplantation: FMT) has been suggested.
　しかしながら、上述した論文（非特許文献１）によれば、動物種によってその年齢や食生活に応じて腸内フローラの種類や状態に特徴や傾向があることが分かってきており、人間に関する便微生物叢移植法の研究成果やノウハウがそのまま適用できないとしている。 However, according to the above-mentioned paper (Non-Patent Document 1), it has been found that the type and condition of the intestinal flora have characteristics and tendencies depending on the animal species according to their age and eating habits. The research results and know-how of the flora transplantation method cannot be applied as they are.
　そこで、本発明は、上記のような問題を解決するものであり、ペット等の動物に適した細菌叢移植用組成物、その製造方法、移植用器具及び移植方法を提供することを目的とする。 Therefore, the present invention solves the above-mentioned problems, and an object of the present invention is to provide a composition for transplanting a bacterial flora suitable for animals such as pets, a method for producing the same, a device for transplantation, and a method for transplantation. ..
　上記課題を解決するために、本発明は、動物から採取された糞便を濾過する濾過工程と、濾過工程で濾過されて得られた糞便細菌叢材料から、遠心分離により細菌叢を抽出する抽出工程と、抽出工程で抽出された細菌叢に色素材料を配合する着色工程とを含む。 In order to solve the above problems, the present invention presents a filtration step of filtering feces collected from animals and an extraction step of extracting the bacterial flora by centrifugation from the fecal bacterial flora material obtained by filtering in the filtration step. And a coloring step of blending a pigment material into the bacterial flora extracted in the extraction step.
　また、本発明は上記製造方法によって製造された細菌叢移植用組成物である。さらに、他の発明は上記製造方法によって製造された細菌叢移植用組成物を移植するために用いられる移植用器具であって、細菌叢移植用組成物を収容し圧縮可能な形状を有する収容部と、収容部に連結された管状の注入部とを備え、注入方向における注入部の、移植対象である動物の体内に挿入される長さが、同方向における収容部の長さよりも長く形成されていることを特徴とする。 Further, the present invention is a composition for transplanting a bacterial flora produced by the above production method. Further, another invention is a transplantation device used for transplanting a bacterial flora transplantation composition produced by the above-mentioned production method, and is a storage unit having a compressible shape for accommodating the bacterial flora transplantation composition. And a tubular injection part connected to the containment part, and the length of the injection part inserted into the body of the animal to be transplanted in the injection direction is formed longer than the length of the containment part in the same direction. It is characterized by being.
　さらに、本発明は、動物から採取された糞便を濾過する濾過工程と、濾過工程で濾過されて得られた糞便細菌叢材料から、遠心分離により細菌叢を抽出する抽出工程と、抽出工程で抽出された細菌叢に色素材料を配合して細菌叢移植用組成物を生成する着色工程と、着色工程で配合された細菌叢移植用組成物を圧縮可能な形状を有する収容部を備えた移植用器具に収容する収容工程と、収容部を圧縮することにより、収容部に連結された管状の注入部を通じて収容部内の細菌叢移植用組成物を投与する投与工程とを含むことを特徴とする細菌叢移植用組成物の移植方法である。 Further, the present invention has a filtration step of filtering feces collected from animals, an extraction step of extracting the bacterial flora by centrifugation from the fecal bacterial flora material obtained by filtering in the filtration step, and an extraction step. For transplantation, which comprises a coloring step of blending a pigment material into the prepared bacterial flora to produce a composition for bacterial flora transplantation, and a container having a compressible shape for the composition for bacterial flora transplantation formulated in the coloring step. Bacteria comprising a containment step of accommodating in an instrument and an administration step of administering the composition for bacterial flora transplantation in the containment portion through a tubular injection portion connected to the accommodating portion by compressing the accommodating portion. This is a method for transplanting a composition for flora transplantation.
　上記細菌叢移植用組成物の移植方法では、投与工程に先行させて、濾過工程で得られた糞便細菌叢材料を凍結させて保管する冷凍保存工程をさらに含み、抽出工程では、凍結された糞便細菌叢材料を解凍し、解凍された糞便細菌叢材料から細菌叢を抽出することが好ましい。 The method for transplanting the composition for transplanting the bacterial flora further includes a cryopreservation step of freezing and storing the stool bacterial flora material obtained in the filtration step prior to the administration step, and the extraction step includes frozen stool. It is preferable to thaw the flora material and extract the flora from the thawed fecal flora material.
　また、上記細菌叢移植用組成物の移植方法では、投与工程に先行させて、抽出工程で抽出された細菌叢を凍結させて保管する冷凍保存工程をさらに含み、着色工程では、凍結された細菌叢を解凍し、解凍された細菌叢から細菌叢移植用組成物を生成するようにしてもよい。 In addition, the method for transplanting the composition for transplanting the bacterial flora further includes a cryopreservation step of freezing and storing the bacterial flora extracted in the extraction step prior to the administration step, and the coloring step includes frozen bacteria. The flora may be thawed to produce a composition for bacterial flora transplantation from the thawed flora.
　さらに、上記細菌叢移植用組成物の移植方法では、投与工程に先行させて、着色工程で生成された細菌叢移植用組成物を凍結させて保管する冷凍保存工程をさらに含み、冷凍保存工程では、収容工程で用いられる収容部に収容して凍結及び保管を行い、収容工程を省略するようにしてもよい。 Further, the method for transplanting the composition for transplanting the bacterial flora further includes a freezing storage step of freezing and storing the composition for transplanting the bacterial flora produced in the coloring step prior to the administration step, and the cryopreservation step further includes. , It may be stored in the storage portion used in the storage step, frozen and stored, and the storage step may be omitted.
　上記冷凍保存工法における凍結はフリーズドライ方式を用いるとともに、このフリーズドライ方式により凍結された材料に水素水を接触させることによって解凍を行うことができる。 The freeze-drying method is used for freezing in the above-mentioned freezing storage method, and the material frozen by this freeze-drying method can be thawed by bringing hydrogen water into contact with the material.
　以上述べたように、これらの発明によれば、動物から採取された糞便を濾過して得られた糞便細菌叢材料から、遠心分離により細菌叢を抽出し、抽出された細菌叢に色素材料を配合するため、適切な細菌叢のみを抽出するとともに、色素によって着色することから、糞便を取り扱う抵抗感を低減しつつ、ペットの体質に適した腸内フローラ移植方法を実施できる。 As described above, according to these inventions, the bacterial flora is extracted by centrifugation from the fecal bacterial flora material obtained by filtering feces collected from animals, and a pigment material is applied to the extracted bacterial flora. Since it is blended, only an appropriate bacterial flora is extracted and colored with a pigment, so that an intestinal flora transplantation method suitable for the constitution of the pet can be carried out while reducing the feeling of resistance to handle feces.
第１実施形態に係る腸内フローラの概要を示す概念図である。It is a conceptual diagram which shows the outline of the intestinal flora which concerns on 1st Embodiment. 第１実施形態に係る細菌叢移植用器具を示す上面図である。It is a top view which shows the microbiota transplantation instrument which concerns on 1st Embodiment. 第２実施形態に係る腸内フローラの概要を示す概念図である。It is a conceptual diagram which shows the outline of the intestinal flora which concerns on 2nd Embodiment. 第２実施形態に係る細菌叢移植用器具を示す上面図である。It is a top view which shows the microbiota transplantation instrument which concerns on 2nd Embodiment.
　［第１実施形態］
 （腸内フローラ移植方法の概要）
　以下に添付図面を参照して、本発明に係る腸内フローラ移植方法の実施形態を詳細に説明する。図１は、本実施形態に係る腸内フローラ移植方法の概要を示す概念図である。なお、以下で説明する処理手順は一例に過ぎず、各処理は可能な限り変更されてもよい。また、以下で説明する処理手順について、実施の形態に応じて、適宜、ステップの省略、置換及び追加が可能である。 [First Embodiment]
(Outline of intestinal flora transplantation method)
Hereinafter, embodiments of the intestinal flora transplantation method according to the present invention will be described in detail with reference to the accompanying drawings. FIG. 1 is a conceptual diagram showing an outline of the intestinal flora transplantation method according to the present embodiment. The processing procedure described below is only an example, and each processing may be changed as much as possible. Further, with respect to the processing procedure described below, steps can be omitted, replaced and added as appropriate according to the embodiment.
　同図に示すように、先ずドナーとなる動物Ａ１から採取された糞便を濾過する濾過工程Ｓ０１を行う。濾過工程Ｓ０１では、ドナーである動物Ａ１から提供された１００グラムの糞便を溶液に混濁させ、この混濁液２を漏斗２１に設置した滅菌ガーゼ２０を通じて５回の濾過を行う。この溶液は２００グラムの生理食塩水に１００グラムのナノバブル水素水を混ぜて生成されている。このナノバブル水素水は交流電気分解によってつくられた溶液であり、１００万分の１ｍｍサイズの気泡を含んでいる。このようにナノバブル水素水を使うことによって、活性酸素の一種であるヒドロキシラジカルを腸内フローラ内で抑制し、製作してから３日程度以内であれば効用を維持して腸内移植が可能となる。 As shown in the figure, first, a filtration step S01 for filtering feces collected from the donor animal A1 is performed. In the filtration step S01, 100 grams of feces provided by the donor animal A1 is turbid in the solution, and the turbid solution 2 is filtered 5 times through the sterile gauze 20 placed in the funnel 21. This solution is produced by mixing 200 grams of saline with 100 grams of nanobubble hydrogen water. This nanobubble hydrogen water is a solution produced by AC electrolysis and contains bubbles having a size of 1 / 1,000,000 mm. By using nanobubble hydrogen water in this way, hydroxyl radicals, which are a type of active oxygen, are suppressed in the intestinal flora, and within about 3 days after production, the utility is maintained and intestinal transplantation is possible. Become.
　次いで、濾過工程Ｓ０１で濾過されて得られた糞便細菌叢材料３から、遠心分離により細菌叢４２を抽出する抽出工程Ｓ０２を行う。この遠心分離は、糞便細菌叢材料３を入れた試験管を遠心分離機４の回転体４０内に設置して回転させることにより、沈澱物４２ｂと上澄み液４２ａとに分離させ、不要なものを除去する。この遠心分離では、遠心分離機１５００／秒の回転速度を５分間継続させ、さらに細かいものを除去する。 Next, an extraction step S02 is performed to extract the bacterial flora 42 by centrifugation from the fecal bacterial flora material 3 obtained by filtering in the filtration step S01. In this centrifugation, a test tube containing the fecal flora material 3 is placed in the rotating body 40 of the centrifuge 4 and rotated to separate the precipitate 42b and the supernatant 42a, and unnecessary ones are separated. Remove. In this centrifugation, the rotation speed of the centrifuge 1500 / sec is continued for 5 minutes to remove finer particles.
　そして、このように不要物が除去することにより抽出された細菌叢４２ｃに色素材料６を配合する着色工程Ｓ０３を行う。ここでは、例えば、色素材料６として、食用黄４号８．４％及び食用青色１号３．６％を配合し緑色に着色する。なお、この着色工程Ｓ０３において細菌叢に配合される色素材料としては、例えば、クチナシの果実から抽出した材料を用いることができる。アカネ科クチナシ属の果実は、古来から生薬や漢方薬の原料として用いられ、腸の中に入っても害はなく、果実に含まれるカロチノイドの一種であるクロシンは黄色の着色料にもなる。また、クチナシの果実の抽出物中に含まれるゲニピンと、米糠に含まれるアミノ酸とを発酵させて青色の着色料を生成し、上記黄色色素７５％と、青色色素２０％とを混ぜて緑に着色し、デキストリンを５％まぜて粉末にする。この粉末を水に溶かし、この粉末の溶液を用いて、細菌叢を濾過する最後の回に使用するようにしてもよい。 Then, a coloring step S03 is performed in which the dye material 6 is blended with the bacterial flora 42c extracted by removing unnecessary substances in this way. Here, for example, as the pigment material 6, 8.4% of edible yellow No. 4 and 3.6% of edible blue No. 1 are blended and colored green. As the pigment material to be blended in the bacterial flora in this coloring step S03, for example, a material extracted from the fruit of gardenia can be used. Fruits of the genus Gardenia of the family Rubiaceae have been used as raw materials for crude drugs and Chinese herbs since ancient times, and they are harmless even if they enter the intestines. Crocin, which is a kind of carotenoid contained in fruits, also serves as a yellow coloring agent. In addition, genipin contained in the gardenia fruit extract and amino acids contained in rice bran are fermented to produce a blue colorant, and 75% of the above yellow pigment and 20% of the blue pigment are mixed to make green. Color and mix 5% dextrin into powder. The powder may be dissolved in water and the solution of the powder may be used for the final round of filtration of the flora.
　次いで、着色工程Ｓ０３で配合された細菌叢移植用組成物５を、移植用器具７の収容部７ａに収容する収容工程Ｓ０４を行う。この収容工程Ｓ０４で用いられる移植用器具７は、上記細菌叢移植用組成物５を移植するために用いられる器具であって、細菌叢移植用組成物５を収容し圧縮可能な形状を有する収容部７ａと、収容部７ａに連結されてその内部が連通された管状の注入部７ｂとを備えている。この移植用器具７は、注入方向における注入部７ｂの、移植対象である動物Ａ２の体内に挿入される長さが、同方向における収容部７ａの長さよりも長く形成されている。 Next, a storage step S04 is performed in which the bacterial flora transplantation composition 5 blended in the coloring step S03 is stored in the storage portion 7a of the transplantation device 7. The transplantation device 7 used in the storage step S04 is a device used for transplanting the above-mentioned bacterial flora transplantation composition 5, and contains the bacterial flora transplantation composition 5 and has a compressible shape. It includes a portion 7a and a tubular injection portion 7b connected to the accommodating portion 7a and communicated with the inside thereof. The transplanting device 7 is formed so that the length of the injection portion 7b inserted into the body of the animal A2 to be transplanted in the injection direction is longer than the length of the accommodating portion 7a in the same direction.
　本実施形態において、移植用器具７の少なくとも収容部７ａは透明の素材で形成されて中身がよく見えるようになっている。また、本実施形態に係る移植用器具７は、収容部７ａの長さが５センチとなっているとともに、管状の注入部７ｂの長さが１０センチ程度に形成されている。そして、図２に拡大して示すように、動物Ａ２の種類によって注入部７ｂの長さが調節できるように１センチ単位でメモリ７ｄが付されているとともに、注入部７ｂの長手方向に沿って移動可能なストッパー７ｃが取付けられており、注入部７ｂの、動物Ａ２の体内に挿入される実質的な長さを変えられるようになっている。最後に、移植対象である動物Ａ２に細菌叢移植用組成物５を投与する投与工程Ｓ０５を実行する。ここでは、注入部７ｂを動物Ａ２の肛門に挿入し、収容部７ａを圧縮することにより注入部７ｂを通じて収容部７ａ内の細菌叢移植用組成物５を投与する。 In the present embodiment, at least the accommodating portion 7a of the transplanting instrument 7 is formed of a transparent material so that the contents can be clearly seen. Further, in the transplanting instrument 7 according to the present embodiment, the length of the accommodating portion 7a is 5 cm, and the length of the tubular injection portion 7b is formed to be about 10 cm. Then, as shown enlarged in FIG. 2, a memory 7d is attached in units of 1 cm so that the length of the injection portion 7b can be adjusted according to the type of the animal A2, and along the longitudinal direction of the injection portion 7b. A movable stopper 7c is attached so that the substantially length of the injection portion 7b inserted into the body of the animal A2 can be changed. Finally, the administration step S05 for administering the composition 5 for microbiota transplantation to the animal A2 to be transplanted is performed. Here, the injection portion 7b is inserted into the anus of the animal A2, and the composition 5 for bacterial flora transplantation in the containment portion 7a is administered through the injection portion 7b by compressing the accommodation portion 7a.
 （作用・効果）
　以上説明した本実施形態にかかる　腸内フローラ移植方法によれば、動物から採取された糞便を濾過して得られた糞便細菌叢材料から、遠心分離により細菌叢を抽出し抽出された細菌叢に色素材料を配合するため、適切な細菌叢のみを抽出するとともに、色素によって着色することによって糞便を取り扱う心理的な抵抗感を低減しつつ、ペットの体質に適した腸内フローラ移植方法を実施できる。 (Action / effect)
According to the intestinal flora transplantation method according to the present embodiment described above, the bacterial flora is extracted by centrifugation from the fecal bacterial flora material obtained by filtering feces collected from animals to obtain the extracted bacterial flora. Since the pigment material is blended, it is possible to carry out an intestinal flora transplantation method suitable for the constitution of the pet while extracting only the appropriate bacterial flora and reducing the psychological resistance to handle feces by coloring with the pigment. ..
　［第２実施形態］
　続いて本発明の第２実施形態について説明する。本実施形態では、上述した第１実施形態で説明した工程に、材料を凍結して保管し、解凍する冷凍保存工程をさらに設けたことを要旨とする。図３は、本実施形態に係る腸内フローラ移植方法の概要を示す概念図である。なお、以下に示す実施の形態は、この発明の技術的思想を具体化するための装置等を例示するものであって、この発明の技術的思想は、各構成部品の材質、形状、構造、配置等を下記のものに特定するものでない。この発明の技術的思想は、特許請求の範囲において、種々の変更を加えることができる。 [Second Embodiment]
Subsequently, the second embodiment of the present invention will be described. The gist of the present embodiment is that the process described in the first embodiment described above is further provided with a freezing storage step of freezing, storing, and thawing the material. FIG. 3 is a conceptual diagram showing an outline of the intestinal flora transplantation method according to the present embodiment. It should be noted that the embodiments shown below exemplify an apparatus or the like for embodying the technical idea of the present invention, and the technical idea of the present invention describes the material, shape, structure, and the material of each component. The arrangement etc. is not specified as the following. The technical idea of the present invention can be modified in various ways within the scope of claims.
 （腸内フローラ移植方法の概要）
　図３に示すように、本実施形態においても上述した第１実施形態と同様に、先ずドナーとなる動物Ａ１から採取された糞便を濾過する濾過工程Ｓ０１を行う。濾過工程Ｓ０１では、ドナーである動物Ａ１から提供された１００グラムの糞便を溶液に混濁させ、この混濁液２を漏斗２１に設置した滅菌ガーゼ２０を通じて５回の濾過を行う。この溶液は２００グラムの生理食塩水に１００グラムのナノバブル水素水を混ぜて生成されている。このナノバブル水素水は交流電気分解によってつくられた溶液であり、１００万分の１ｍｍサイズの気泡を含んでいる。このようにナノバブル水素水を使うことによって、活性酸素の一種であるヒドロキシラジカルを腸内フローラ内で抑制し、製作してから３日程度以内であれば効用を維持して腸内移植が可能となる。 (Outline of intestinal flora transplantation method)
As shown in FIG. 3, in the present embodiment as well, in the same manner as in the first embodiment described above, first, the filtration step S01 for filtering the feces collected from the donor animal A1 is performed. In the filtration step S01, 100 grams of feces provided by the donor animal A1 is turbid in the solution, and the turbid solution 2 is filtered 5 times through the sterile gauze 20 placed in the funnel 21. This solution is produced by mixing 200 grams of saline with 100 grams of nanobubble hydrogen water. This nanobubble hydrogen water is a solution produced by AC electrolysis and contains bubbles having a size of 1 / 1,000,000 mm. By using nanobubble hydrogen water in this way, hydroxyl radicals, which are a type of active oxygen, are suppressed in the intestinal flora, and within about 3 days after production, the utility is maintained and intestinal transplantation is possible. Become.
　次いで、濾過工程Ｓ０１で濾過されて得られた糞便細菌叢材料３から、遠心分離により細菌叢４２を抽出する抽出工程Ｓ０２を行う。この遠心分離は、糞便細菌叢材料３を入れた試験管を遠心分離機４の回転体４０内に設置して回転させることにより、沈澱物４２ｂと上澄み液４２ａとに分離させ、不要なものを除去する。この遠心分離では、遠心分離機１５００／秒の回転速度を５分間継続させ、さらに細かいものを除去する。 Next, an extraction step S02 is performed to extract the bacterial flora 42 by centrifugation from the fecal bacterial flora material 3 obtained by filtering in the filtration step S01. In this centrifugation, a test tube containing the fecal flora material 3 is placed in the rotating body 40 of the centrifuge 4 and rotated to separate the precipitate 42b and the supernatant 42a, and unnecessary ones are separated. Remove. In this centrifugation, the rotation speed of the centrifuge 1500 / sec is continued for 5 minutes to remove finer particles.
　そして、このように不要物が除去することにより抽出された細菌叢４２ｃに色素材料６を配合する着色工程Ｓ０３を行う。ここでは、例えば、色素材料６として、食用黄４号８．４％及び食用青色１号３．６％を配合し緑色に着色する。なお、この着色工程Ｓ０３において細菌叢に配合される色素材料としては、例えば、クチナシの果実から抽出した材料を用いることができる。アカネ科クチナシ属の果実は、古来から生薬や漢方薬の原料として用いられ、腸の中に入っても害はなく、果実に含まれるカロチノイドの一種であるクロシンは黄色の着色料にもなる。また、クチナシの果実の抽出物の中に含まれるゲニピンと、米糠に含まれるアミノ酸とを発酵させて青色の着色料を生成し、上記黄色色素７５％と、青色色素２０％とを混ぜて緑に着色し、デキストリンを５％まぜて粉末にする。この粉末を水に溶かし、この粉末の溶液を用いて、細菌叢を濾過する最後の回に使用するようにしてもよい。 Then, a coloring step S03 is performed in which the dye material 6 is blended with the bacterial flora 42c extracted by removing unnecessary substances in this way. Here, for example, as the pigment material 6, 8.4% of edible yellow No. 4 and 3.6% of edible blue No. 1 are blended and colored green. As the pigment material to be blended in the bacterial flora in this coloring step S03, for example, a material extracted from the fruit of gardenia can be used. Fruits of the genus Gardenia of the family Rubiaceae have been used as raw materials for crude drugs and Chinese herbs since ancient times, and they are harmless even if they enter the intestines. Crocin, which is a kind of carotenoid contained in fruits, also serves as a yellow coloring agent. In addition, genipin contained in the gardenia fruit extract and amino acids contained in rice bran are fermented to produce a blue colorant, and 75% of the above yellow pigment and 20% of the blue pigment are mixed to produce green. And mix 5% of dextrin into powder. The powder may be dissolved in water and the solution of the powder may be used for the final round of filtration of the flora.
　ここで、本実施形態では、投与工程Ｓ０５に先行させて、着色工程Ｓ０３で生成された細菌叢移植用組成物を凍結させて保管する冷凍保存工程Ｓ６１を行う。この冷凍保存工程Ｓ６１では、収容工程Ｓ０４で用いられる収容容器８の収容部８ａに細菌叢移植用組成物に収容した状態で凍結及び保管を行う。 Here, in the present embodiment, prior to the administration step S05, a freezing storage step S61 is performed in which the composition for bacterial flora transplantation produced in the coloring step S03 is frozen and stored. In this freezing storage step S61, freezing and storage are performed in a state of being contained in the composition for transplanting the bacterial flora in the storage portion 8a of the storage container 8 used in the storage step S04.
　この冷凍保存工程Ｓ６１における凍結はフリーズドライ方式を用いる。具体的には、細菌叢移植用組成物（腸内フローラ液）１００ccを８時間で３０％減量されるように、すなわち７０ccとなるようにフリーズドライ処理を行う。そして、この凍結された細菌叢移植用組成物（腸内フローラ液）については、収集して分析する工程Ｓ６２を行う。この収集及び分析の結果、投与対象の動物Ａ２の種類や症状に応じて適切な細菌叢移植用組成物（腸内フローラ液）を選択できるようになる。なお、ここで収集される細菌叢移植用組成物（腸内フローラ液）としては、同種の動物同士のものに限らず、例えば病気や疾患を有する他の小動物の細菌叢移植用組成物、ペットのオーナーなどの生活環境が近似する者の腸内フローラも含めることができ、特定の病状に特化した細菌叢が多く含まれるフローラを選択して治療に用いるようにしてもよい。 A freeze-drying method is used for freezing in this freezing storage step S61. Specifically, freeze-drying treatment is performed so that 100 cc of the composition for transplanting the bacterial flora (intestinal flora solution) is reduced by 30% in 8 hours, that is, 70 cc. Then, the frozen bacterial flora transplantation composition (intestinal flora solution) is collected and analyzed in step S62. As a result of this collection and analysis, it becomes possible to select an appropriate composition for bacterial flora transplantation (intestinal flora solution) according to the type and symptom of the animal A2 to be administered. The microbiota transplantation composition (intestinal flora solution) collected here is not limited to those of animals of the same species, for example, microbiota transplantation compositions of other small animals having a disease or disease, pets. The intestinal flora of a person whose living environment is similar, such as the owner of the animal, may be included, and a flora containing a large amount of bacterial flora specialized for a specific medical condition may be selected and used for treatment.
　また、冷凍保存工程Ｓ６１においては、液体窒素を用いて細菌叢移植用組成物（腸内フローラ液）を凍結して、長期間保存するようにしてもよい。この場合には、投与対象の動物Ａ２が若いときや健康状態にあるときの腸内フローラをフリーズドライ或いは液体窒素により凍結させて保管し、老齢化したり病気になったときに、凍結させておいた腸内フローラを解凍して、投与するようにしてもよい。 Further, in the frozen storage step S61, the composition for transplanting the bacterial flora (intestinal flora solution) may be frozen using liquid nitrogen and stored for a long period of time. In this case, the intestinal flora when the animal A2 to be administered is young or in a healthy state is frozen and stored by freeze-drying or liquid nitrogen, and frozen when it becomes old or sick. The intestinal flora that has been present may be thawed and administered.
　特に、本実施形態では、着色工程Ｓ０３で配合された細菌叢移植用組成物５を、移植用器具８の収容部８ａに収容して、冷凍保存Ｓ６１を行う。この移植用器具８は、上記細菌叢移植用組成物５を移植するために用いられる器具であって、細菌叢移植用組成物５を収容し圧縮可能な形状を有する収容部８ａと、収容部８ａに連結されてその内部が連通された管状の注入部８ｂとを備えている。この移植用器具８は、注入方向における注入部８ｂの、移植対象である動物Ａ２の体内に挿入される長さが、同方向における収容部８ａの長さよりも長く形成されている。 In particular, in the present embodiment, the bacterial flora transplantation composition 5 formulated in the coloring step S03 is housed in the storage part 8a of the transplantation device 8 and frozen and stored S61. The transplanting instrument 8 is an instrument used for transplanting the above-mentioned bacterial flora transplanting composition 5, and is an accommodating portion 8a that accommodates the bacterial flora transplanting composition 5 and has a compressible shape, and an accommodating portion. It is provided with a tubular injection portion 8b connected to 8a and communicated with the inside thereof. The transplanting device 8 is formed so that the length of the injection portion 8b inserted into the body of the animal A2 to be transplanted in the injection direction is longer than the length of the accommodating portion 8a in the same direction.
　本実施形態において、移植用器具８の少なくとも収容部８ａは透明の素材で形成されて中身がよく見えるようになっているとともに、蛇腹状をなして軸方向に伸縮可能に形成されている。また、本実施形態に係る移植用器具８は、収容部８ａの長さが５センチとなっているとともに、管状の注入部８ｂの長さが１０センチ程度に形成されている。そして、図４に拡大して示すように、動物Ａ２の種類によって注入部８ｂの長さが調節できるように１センチ単位で目盛８ｄが付されているとともに、注入部８ｂの長手方向に沿って移動可能なストッパー８ｃが取付けられており、注入部８ｂの、動物Ａ２の体内に挿入される実質的な長さを変えられるようになっている。 In the present embodiment, at least the accommodating portion 8a of the transplanting instrument 8 is formed of a transparent material so that the contents can be clearly seen, and is formed in a bellows shape so as to be expandable and contractible in the axial direction. Further, in the transplanting instrument 8 according to the present embodiment, the length of the accommodating portion 8a is 5 cm, and the length of the tubular injection portion 8b is formed to be about 10 cm. Then, as shown in an enlarged manner in FIG. 4, a scale 8d is provided in units of 1 cm so that the length of the injection portion 8b can be adjusted according to the type of the animal A2, and along the longitudinal direction of the injection portion 8b. A movable stopper 8c is attached so that the substantially length of the injection portion 8b inserted into the body of the animal A2 can be changed.
　さらに、注入部８ｂの後端部にはキャップ８３が連通されており、収容部８ａに螺合されて連結できるようになっている。また、この収容部８ａは別途、閉止用のキャップ８ｃも螺合可能となっており、この閉止用のキャップ８ｃを使うことによって冷凍保存する際には注入部８ｂを取り外して冷凍保存に適した形とすることができる。 Further, a cap 83 is communicated with the rear end portion of the injection portion 8b so that it can be screwed into the accommodating portion 8a and connected. In addition, the accommodating portion 8a can also be screwed into a cap 8c for closing separately, and by using the cap 8c for closing, the injection portion 8b is removed and suitable for freezing storage. Can be shaped.
　このように収集され保管された細菌叢移植用組成物（腸内フローラ液）の中から適切なものを選択肢、解凍工程Ｓ６３を行う。この解凍工程Ｓ６３では、例えば人肌温度（３６℃前後）や室温等で緩やかに解凍することが好ましい。また、凍結された材料に水素水を接触させることによって解凍を行うようにしてもよい。最後に、移植対象である動物Ａ２に細菌叢移植用組成物５を投与する投与工程Ｓ０５を実行する。ここでは、注入部７ｂを動物Ａ２の肛門に挿入し、収容部７ａを圧縮することにより注入部７ｂを通じて収容部７ａ内の細菌叢移植用組成物５を投与する。 From the composition for transplanting the bacterial flora (intestinal flora solution) collected and stored in this way, an appropriate one is selected, and the thawing step S63 is performed. In this thawing step S63, it is preferable to thawing gently at, for example, human skin temperature (around 36 ° C.) or room temperature. Further, the frozen material may be thawed by bringing hydrogen water into contact with the frozen material. Finally, the administration step S05 for administering the composition 5 for microbiota transplantation to the animal A2 to be transplanted is performed. Here, the injection portion 7b is inserted into the anus of the animal A2, and the composition 5 for bacterial flora transplantation in the containment portion 7a is administered through the injection portion 7b by compressing the accommodation portion 7a.
 （変更例）
　なお、以上説明した実施形態の説明は、本発明の一例である。このため、本発明は上述した実施形態に限定されることなく、本発明に係る技術的思想を逸脱しない範囲であれば、設計等に応じて種々の変更が可能である。 (Change example)
The description of the embodiments described above is an example of the present invention. Therefore, the present invention is not limited to the above-described embodiment, and various modifications can be made according to the design and the like as long as the technical idea of the present invention is not deviated.
　例えば、上述した実施形態では、着色工程Ｓ０３で生成された細菌叢移植用組成物（腸内フローラ液）を冷凍保存するようにしたが、濾過工程Ｓ０１で得られた糞便細菌叢材料３を凍結させて保管しておき、抽出工程Ｓ０２では、その凍結された糞便細菌叢材料を解凍し、解凍された糞便細菌叢材料から細菌叢を抽出するようにしてもよい。或いは、抽出工程Ｓ０２で抽出された細菌叢を凍結させて保管しておき、着色工程Ｓ０３では、その凍結された細菌叢を解凍し、解凍された細菌叢から細菌叢移植用組成物を生成するようにしてもよい。 For example, in the above-described embodiment, the composition for transplanting the bacterial flora (intestinal flora solution) produced in the coloring step S03 is frozen and stored, but the fecal flora material 3 obtained in the filtering step S01 is frozen. In the extraction step S02, the frozen fecal flora material may be thawed and the flora may be extracted from the thawed fecal flora material. Alternatively, the bacterial flora extracted in the extraction step S02 is frozen and stored, and in the coloring step S03, the frozen bacterial flora is thawed and a composition for transplanting the bacterial flora is produced from the thawed bacterial flora. You may do so.
 （作用・効果）
　以上説明した本実施形態にかかる　腸内フローラ移植方法によれば、動物から採取された糞便を濾過して得られた糞便細菌叢材料から、遠心分離により細菌叢を抽出し抽出された細菌叢に色素材料を配合するため、適切な細菌叢のみを抽出するとともに、色素によって着色することによって糞便を取り扱う心理的な抵抗感を低減しつつ、ペットの体質に適した腸内フローラ移植方法を実施できる。 (Action / effect)
According to the intestinal flora transplantation method according to the present embodiment described above, the bacterial flora is extracted by centrifugation from the fecal bacterial flora material obtained by filtering feces collected from animals to obtain the extracted bacterial flora. Since the pigment material is blended, it is possible to carry out an intestinal flora transplantation method suitable for the constitution of the pet while extracting only the appropriate bacterial flora and reducing the psychological resistance to handle feces by coloring with the pigment. ..
　特に、本実施形態では、腸内フローラを冷凍保存して収集・分析することによって、特定の病気や疾患に特化した腸内フローラーを適宜選別して提供できるドナーバンク的なサービスを提供することができる。例えば、移植する腸内フローラのドナーについて、収集した腸内フローラーの一部を最初に冷凍保存しておき、２ケ月の間隔で生体試料分析を専門機関において実施することができ、生体試料分析で安全が確認されてから最初に収集し冷凍保存しておいたフローラを解凍して使用することにより、安全性を向上させることができる。 In particular, in the present embodiment, by storing and analyzing the intestinal flora in a frozen state, a donor bank-like service capable of appropriately selecting and providing an intestinal flora specialized for a specific disease or disease is provided. Can be done. For example, for a donor of intestinal flora to be transplanted, a part of the collected intestinal flora can be stored frozen first, and biological sample analysis can be performed at a specialized institution at intervals of 2 months. Safety can be improved by thawing and using the flora that was first collected and stored frozen after the safety was confirmed.
　なお、本発明は、上記した各実施の形態そのままに限定されるものではなく、実施段階ではその要旨を逸脱しない範囲で構成要素を変形して具体化できる。また、上記実施の形態に開示されている複数の構成要素の適宜な組み合せにより、種々の発明を形成できる。例えば、実施の形態に示される全構成要素から幾つかの構成要素を削除してもよい。 It should be noted that the present invention is not limited to the above-described embodiments as they are, and at the implementation stage, the components can be modified and embodied within a range that does not deviate from the gist thereof. In addition, various inventions can be formed by an appropriate combination of the plurality of components disclosed in the above-described embodiment. For example, some components may be removed from all the components shown in the embodiments.
　Ａ１，Ａ２…動物
　２…混濁液
　３…糞便細菌叢材料
　４…遠心分離機
　５…細菌叢移植用組成物
　６…色素材料
　７，８…移植用器具
　７ａ，８ａ…収容部
　７ｂ，８ｂ…注入部
　２０…滅菌ガーゼ
　２１…漏斗
　４０…回転体
　４２…細菌叢
　４２ａ…上澄み液
　４２ｂ…沈澱物
　４２ｃ…細菌叢 A1, A2 ... Animal 2 ... Turbid liquid 3 ... Fecal flora material 4 ... Centrifuge 5 ... Bacterial flora transplantation composition 6 ... Dye material 7,8 ... Transplanting equipment 7a, 8a ... Containment part 7b, 8b ... Injection Part 20 ... Sterilized gauze 21 ... Funnel 40 ... Rotating body 42 ... Bacterial flora 42a ... supernatant 42b ... Precipitate 42c ... Bacterial flora

Claims (8)

  1. 　動物から採取された糞便を濾過する濾過工程と、
    　前記濾過工程で濾過されて得られた糞便細菌叢材料から、遠心分離により細菌叢を抽出する抽出工程と、
    　前記抽出工程で抽出された細菌叢に色素材料を配合する着色工程と
    を含むことを特徴とする細菌叢移植用組成物の製造方法。     A filtration process that filters feces collected from animals,
    An extraction step of extracting the bacterial flora by centrifugation from the fecal bacterial flora material obtained by filtering in the filtration step, and
    A method for producing a composition for transplanting a bacterial flora, which comprises a coloring step of blending a pigment material with the bacterial flora extracted in the extraction step.
  2. 　請求項１に記載の製造方法によって製造された細菌叢移植用組成物。 A composition for transplanting a bacterial flora produced by the production method according to claim 1.
  3. 　請求項１に記載の製造方法によって製造された細菌叢移植用組成物を移植するために用いられる移植用器具であって、
    　前記細菌叢移植用組成物を収容し圧縮可能な形状を有する収容部と、
    　前記収容部に連結された管状の注入部と
    を備え、
    　注入方向における前記注入部の、移植対象である動物の体内に挿入される長さが、同方向における前記収容部の長さよりも長く形成されている
    ことを特徴とする移植用器具。     An instrument for transplantation used for transplanting a composition for transplanting a bacterial flora produced by the production method according to claim 1.
    An accommodating portion accommodating the composition for transplanting the bacterial flora and having a compressible shape,
    A tubular injection section connected to the housing section is provided.
    An instrument for transplantation, characterized in that the length of the injection portion inserted into the body of the animal to be transplanted in the injection direction is formed longer than the length of the accommodation portion in the same direction.
  4. 　動物から採取された糞便を濾過する濾過工程と、
    　前記濾過工程で濾過されて得られた糞便細菌叢材料から、遠心分離により細菌叢を抽出する抽出工程と、
    　前記抽出工程で抽出された細菌叢に色素材料を配合して細菌叢移植用組成物を生成する着色工程と、
    　前記着色工程で配合された細菌叢移植用組成物を圧縮可能な形状を有する収容部を備えた移植用器具に収容する収容工程と、
    　前記収容部を圧縮することにより、前記収容部に連結された管状の注入部を通じて前記収容部内の前記細菌叢移植用組成物を投与する投与工程と
    を含むことを特徴とする細菌叢移植用組成物の移植方法。     A filtration process that filters feces collected from animals,
    An extraction step of extracting the bacterial flora by centrifugation from the fecal bacterial flora material obtained by filtering in the filtration step, and
    A coloring step of blending a pigment material with the bacterial flora extracted in the extraction step to produce a composition for transplanting the bacterial flora, and
    A storage step of accommodating the bacterial flora transplantation composition formulated in the coloring step in a transplantation device having a storage portion having a compressible shape, and a storage step.
    A composition for bacterial flora transplantation, which comprises an administration step of administering the composition for transplanting the bacterial flora in the accommodating portion through a tubular injection portion connected to the accommodating portion by compressing the accommodating portion. How to transplant things.
  5. 　前記投与工程に先行させて、前記濾過工程で得られた糞便細菌叢材料を凍結させて保管する冷凍保存工程をさらに含み、前記抽出工程では、凍結された糞便細菌叢材料を解凍し、解凍された糞便細菌叢材料から細菌叢を抽出することを特徴とする請求項４に記載の細菌叢移植用組成物の移植方法。 Prior to the administration step, a cryopreservation step of freezing and storing the fecal flora material obtained in the filtration step is further included, and in the extraction step, the frozen fecal flora material is thawed and thawed. The method for transplanting a composition for transplanting a bacterial flora according to claim 4, wherein the bacterial flora is extracted from the material of the stool bacterial flora.
  6. 　前記投与工程に先行させて、前記抽出工程で抽出された細菌叢を凍結させて保管する冷凍保存工程をさらに含み、前記着色工程では、凍結された細菌叢を解凍し、解凍された細菌叢から細菌叢移植用組成物を生成することを特徴とする請求項４に記載の細菌叢移植用組成物の移植方法。 Prior to the administration step, a cryopreservation step of freezing and storing the bacterial flora extracted in the extraction step is further included, and in the coloring step, the frozen bacterial flora is thawed and the thawed bacterial flora is used. The method for transplanting a composition for transplanting a bacterial flora according to claim 4, wherein the composition for transplanting a bacterial flora is produced.
  7. 　前記投与工程に先行させて、前記着色工程で生成された細菌叢移植用組成物を凍結させて保管する冷凍保存工程をさらに含み、
    　前記冷凍保存工程では、前記収容工程で用いられる前記収容部に収容して凍結及び保管を行い、前記収容工程を省略する
    ことを特徴とする請求項４に記載の細菌叢移植用組成物の移植方法。     Prior to the administration step, a freezing storage step of freezing and storing the composition for bacterial flora transplantation produced in the coloring step is further included.
    The transplantation of the composition for bacterial flora transplantation according to claim 4, wherein in the freezing storage step, the composition is stored in the storage portion used in the storage step, frozen and stored, and the storage step is omitted. Method.
  8. 　前記冷凍保存工程における凍結はフリーズドライ方式を用いるとともに、このフリーズドライ方式により凍結された材料に水素水を接触させることによって前記解凍を行うことを特徴とする請求項５乃至７のいずれかに記載の細菌叢移植用組成物の移植方法。 The method according to any one of claims 5 to 7, wherein the freezing in the freezing storage step uses a freeze-drying method, and the thawing is performed by bringing hydrogen water into contact with the material frozen by the freeze-drying method. A method for transplanting a composition for transplanting a bacterial flora.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022158244A1 (en) * 2021-01-21 2022-07-28 株式会社晩聲社 Composition for bacterial flora transplantation, method for producing same, and method for transplanting same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6264549U (en) * 1985-10-14 1987-04-22
JPH04126552U (en) * 1991-05-09 1992-11-18 正章 羽賀 liquid injection container
JPH0668759U (en) * 1993-03-15 1994-09-27 東豊薬品株式会社 Enema container
JP2013537531A (en) * 2010-08-04 2013-10-03 トーマス・ジュリアス・ボロディ Composition for faecal flora transplantation and methods for making and using it and device for delivering the same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5629805B2 (en) * 2013-05-07 2014-11-26 株式会社かがやき Nanobubble manufacturing method and nanobubble manufacturing apparatus
JP2015127301A (en) * 2013-12-27 2015-07-09 ライオン株式会社 Visceral fat reducing agent and fat synthesis inhibiting agent comprising hydrogen nanobubble water
CN106715703A (en) * 2014-09-17 2017-05-24 帝斯曼知识产权资产管理有限公司 Process for producing gardenia blue pigment

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6264549U (en) * 1985-10-14 1987-04-22
JPH04126552U (en) * 1991-05-09 1992-11-18 正章 羽賀 liquid injection container
JPH0668759U (en) * 1993-03-15 1994-09-27 東豊薬品株式会社 Enema container
JP2013537531A (en) * 2010-08-04 2013-10-03 トーマス・ジュリアス・ボロディ Composition for faecal flora transplantation and methods for making and using it and device for delivering the same

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KABAYAMA, SHIGERU ET AL.: "How to take alkaline ionic water and electrolytic hydrogen water wisely", KIDNEY AND DIALYSIS, vol. 85, no. 5, 2018, pages 698 - 702 *
MASUOKA, HIROAKI ET AL.: "Transition of the intestinal microbiota of cats with age", PLOS ONE, vol. 12, no. 8, 2017, pages e018173g, XP055743300 *
REDFERN, ALANA ET AL.: "Role of the gastrointestinal microbiota in small animal health and disease", VETERINARY RECORD, vol. 181, no. 14, 2017 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022158244A1 (en) * 2021-01-21 2022-07-28 株式会社晩聲社 Composition for bacterial flora transplantation, method for producing same, and method for transplanting same

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