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WO2020009897A1 - Canaux fluidiques comprenant un capteur de conductivité et leurs procédés d'utilisation - Google Patents

Canaux fluidiques comprenant un capteur de conductivité et leurs procédés d'utilisation Download PDF

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Publication number
WO2020009897A1
WO2020009897A1 PCT/US2019/039470 US2019039470W WO2020009897A1 WO 2020009897 A1 WO2020009897 A1 WO 2020009897A1 US 2019039470 W US2019039470 W US 2019039470W WO 2020009897 A1 WO2020009897 A1 WO 2020009897A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
channel
liquid
fluidic
resistance
Prior art date
Application number
PCT/US2019/039470
Other languages
English (en)
Inventor
James Russell Webster
Original Assignee
Qorvo Us, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/120,224 external-priority patent/US11344878B2/en
Application filed by Qorvo Us, Inc. filed Critical Qorvo Us, Inc.
Priority to CN201980045635.4A priority Critical patent/CN112469998B/zh
Priority to EP19831197.9A priority patent/EP3818370A4/fr
Priority to JP2021500212A priority patent/JP7518060B2/ja
Publication of WO2020009897A1 publication Critical patent/WO2020009897A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/02—Analysing fluids
    • G01N29/036—Analysing fluids by measuring frequency or resonance of acoustic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/02—Analysing fluids
    • G01N29/022—Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/22—Details, e.g. general constructional or apparatus details
    • G01N29/222—Constructional or flow details for analysing fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2291/00—Indexing codes associated with group G01N29/00
    • G01N2291/02—Indexing codes associated with the analysed material
    • G01N2291/025—Change of phase or condition
    • G01N2291/0255—(Bio)chemical reactions, e.g. on biosensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2291/00—Indexing codes associated with group G01N29/00
    • G01N2291/02—Indexing codes associated with the analysed material
    • G01N2291/025—Change of phase or condition
    • G01N2291/0256—Adsorption, desorption, surface mass change, e.g. on biosensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2291/00—Indexing codes associated with group G01N29/00
    • G01N2291/04—Wave modes and trajectories
    • G01N2291/042—Wave modes
    • G01N2291/0426—Bulk waves, e.g. quartz crystal microbalance, torsional waves

Definitions

  • Diagnostic testing traditionally requires long response times to obtain meaningful data, involves expensive remote or cumbersome laboratory equipment, requires large sample size, utilizes multiple reagents, demands highly trained users, and can involve significant direct and indirect costs.
  • diagnostic testing traditionally requires long response times to obtain meaningful data, involves expensive remote or cumbersome laboratory equipment, requires large sample size, utilizes multiple reagents, demands highly trained users, and can involve significant direct and indirect costs.
  • most tests require that a sample be collected from a patient and then sent to a laboratory, where the results are not available for several hours or days. As a result, the caregiver must wait to treat the patient.
  • Also disclosed are methods of using systems including: a first portion, the first portion including: at least one fluid channel; a fluid actuator; an analysis sensor disposed within the fluid channel; a conductivity sensor disposed within the fluid channel; and an introducer; a second portion, the second portion including: at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel, the method including: monitoring a resistance of the conductivity sensor; moving a liquid within the fluidic channel; and monitoring a change in the resistance of the conductivity sensor.
  • FIG. 1 is a schematic diagram illustrating an illustrative sensor assembly.
  • the term“or” is generally employed in its sense including“and/or” unless the content clearly dictates otherwise.
  • the term“and/or” means one or all of the listed elements or a combination of any two or more of the listed elements.
  • “have”,“having”,“include”,“including”,“comprise”,“comprising” or the like are used in their open ended sense, and generally mean“including, but not limited to”. It will be understood that“consisting essentially of’,“consisting of’, and the like are subsumed in “comprising” and the like.
  • compositions, product, method or the like means that the components of the composition, product, method or the like are limited to the enumerated components and any other components that do not materially affect the basic and novel characteristic(s) of the composition, product, method or the like.
  • any direction referred to herein, such as“top,”“bottom,”“left,” “right,” “upper,” “lower,” and other directions and orientations are described herein for clarity in reference to the figures and are not to be limiting of an actual device or system or use of the device or system. Devices or systems as described herein may be used in a number of directions and orientations.
  • Disclosed devices include a conductivity sensor that can determine the conductivity of at least a portion of the fluid within the device it is located in.
  • the conductivity can be utilized for various purposes.
  • assemblies can include a first portion and a second portion.
  • the first and second portions can be configured to be assembled together to form an assembly.
  • the first and second portions can be assembled together by a manufacturer, an assembler, an end-user, or any combination thereof.
  • the assembly of the two portions can be facilitated by the shape of the two portions, components of at least one of the two portions that are designed to facilitate assembly, or some combination thereof.
  • the two portions can be made of the same material(s) or different materials.
  • the first portion and the second portion can be made of different materials, which because of the different purposes of the two portions, may be useful.
  • the two portions of the assembly can be manufactured separately, in the same or different facilities; and/or can be packaged and/or sold separately or together.
  • At least one of the first and second portions is moveable with respect to the other. This implies that after the first and second portion are assembled to form the assembly, one portion is moveable with respect to the other.
  • the portion that is moveable with respect to the other can be moveable in one or more directions or dimensions. Movement of one portion with respect to the other may offer advantages in that wells in the second portion (discussed below) can be randomly accessed by the first portion. The ability to randomly access the wells in the second portion can allow a large breadth of protocols to be accomplished without altering the configuration of the assembly itself. Other possible advantages provided by the movability of one portion with respect to the other portion are discussed throughout this disclosure.
  • FIG. 1 illustrates an illustrative embodiment of an assembly.
  • This illustrative assembly 100 includes a first portion 110 and a second portion 120.
  • This particular illustrative assembly 100 is configured to be assembled in a way that positions the second portion 120 below the first portion 110 in the z direction.
  • the second portion 120 is moveable with respect to the first portion 110.
  • the second portion being moveable with respect to the first portion can imply that the second portion can move in at least one dimension (x, y, or z) with respect to the first portion, which is stationary.
  • the second portion can move along a straight line with respect to the first portion (for example, along the x dimension).
  • the second portion 120 can move along a straight line with respect to the first portion (for example along the x dimension) and can move up and down with respect to the first portion (for example along the z dimension). Such movement could be seen in the assembly 100 if the second portion 120 also moved in the z dimension.
  • the first portion can include at least one fluidic pathway, a fluid actuator, and an introducer.
  • Fluidic pathways can also be described as including a fluid channel.
  • the illustrative first portion 110 illustrated in FIG. 1 includes a fluid channel 112, a fluid actuator 114, and an introducer 116.
  • the fluid channel 112, the fluid actuator 114, and the introducer 116 are in fluid communication with one another. It can also be described that the fluid actuator 114, the introducer 116, and the fluid channel 112 are within, on, or are part of the fluidic pathway.
  • the fluidic pathway can have various configurations, and the examples depicted herein serve only as illustrative configurations.
  • the fluidic pathway does not include portions of the device that obtain the sample.
  • the fluidic pathway begins after a sample is contained in a well of the second portion.
  • the fluidic pathway can be described as a transit path for fluids in the assembly.
  • the fluidic pathway need not be fluidly connected at all times.
  • the fluidic pathway can include a portion of the device that can be (based may be moved into or out of the fluid pathway, by for example moving one portion with respect to another portion.
  • the fluidic pathway can also be described as including any portion of the device accessible by the introducer, any portion of the device fluidly connected with any portion of the device accessible by the introducer, or some combination thereof.
  • the fluidic pathway need not include only an actual volume that is connected.
  • a fluidic pathway can be entirely housed on a first portion, entirely housed on a second portion, or at least one portion of a fluidic pathway can exist on a first and at least one portion of a fluidic pathway can exist on a second portion.
  • a fluidic pathway can be one that is connected at all times and in some embodiments, one or more than one portion of a fluidic pathway can be at some times disconnected from the remainder of the fluidic pathway.
  • a fluidic pathway can include a fluid channel.
  • such a fluid channel can be a volume that is connected at all times.
  • such a fluid channel can be entirely housed on the first portion of an assembly.
  • such a fluid channel can be entirely housed on the first portion of an assembly can be a volume that is statically connected at all times.
  • a fluid channel can refer to a physical channel on a first portion of an assembly.
  • the fluidic pathway does not include valves.
  • the fluid channel does not include valves.
  • fluid can flow in either direction in the fluidic pathway (or in the fluid channel) even though there are no valves. Bi-directional flow is possible, even though there may be no valves in the fluidic pathway (or the fluid channel) because of the ability to randomly access wells (for example an empty well) in the second portion.
  • two directional flow can be accomplished by depositing liquid (in some embodiments all the liquid) in the fluidic pathway (or the fluid channel) in an empty well on the second portion by flowing the fluid in a first direction and then retrieving that liquid from that well and directing it in the fluidic pathway by flowing the fluid in a second direction (opposite to the first direction).
  • liquid in some embodiments all the liquid
  • second direction opposite to the first direction
  • Fluidic pathways can have access to a sample introduction pathway as well.
  • the sample introduction pathway and the fluidic pathway need not be entirely located on or in the same portion.
  • the sample introduction pathway can include one or more than one component that can function to get a sample into a well.
  • the sample introduction pathway can be described as a transit path for the sample before it is in a well.
  • the sample introduction pathway need not be fluidly connected at all times.
  • the sample introduction pathway can include a portion of the device that can be (based on for example movement of one portion with respect to the other portion) moved into or out of the sample introduction pathway.
  • the sample introduction pathway can include, for example a sample introduction chamber and one or more than one component to get a sample from the sample introduction chamber to a well
  • sample introduction pathway can include one or more than one irreversible valve.
  • a valve or valves that may be in the sample introduction pathway can also be described as not including moving parts.
  • the sample introduction chamber can be located on or in the first portion.
  • the sample introduction pathway can for example include a valve(s), a filter(s), or some combination thereof.
  • the sample introduction pathway can utilize the introducer portion of the first portion.
  • the sample can be moved from a sample introduction chamber to a sample well on the second portion.
  • a sample introduction pathway can be configured to introduce sample directly into a fluidic pathway or a fluid channel that is part of a fluidic pathway.
  • the sample introduction pathway would be configured to deposit a sample into the fluidic pathway without first depositing it into a sample well.
  • Such configurations could be especially useful or applicable to instances where the sample size is relatively small.
  • such configurations could be utilized for sample sizes of not greater than 100 pL, for example.
  • An example of such a sample could include a quantity of blood obtained via a finger prick.
  • FIG. 1 shows a fluid channel 112 that is part of the fluidic pathway.
  • the fluid channel 112 can be formed (i.e., top, bottom and sides) from more than one component or piece of the first portion. In some embodiments, the fluid channel 112 does not contain any fluid valves.
  • Illustrative fluid channels can be described by their volumes, either by their total volumes or by the volume both before and after the analysis sensor.
  • illustrative fluid channels can have volumes of 10 pL to 1000 pL in the region before the analysis sensor and 10 pL to 1000 pL in the region after the analysis sensor.
  • illustrative fluid channels can have volumes of 50 pL to 250 pL in the region before the analysis sensor and 50 pL to 250 pL in the region after the analysis sensor.
  • illustrative fluid channels can have volumes of 75 pL to 200 pL in the region before the analysis sensor and 75 pL to 200 pL in the region after the analysis sensor.
  • illustrative fluid channels can have volumes of 100 pL to 175 pL in the region before the analysis sensor and 100 pL to 175 pL in the region after the analysis sensor. It should also be understood that the volumes before and after the analysis sensor need not be the same.
  • the first portion also includes a fluid actuator 114.
  • fluid actuator 114 is depicted as being at one end of the fluid channel 112, it should be understood that a fluid actuator could be located at any point along the fluid channel 112, could be located at multiple points along the fluid channel 112, and/or could have multiple components at multiple points along the fluid channel 112.
  • the fluid actuator 114 functions to move fluid along the fluid channel 112. It can also be described that the fluid actuator 114 functions to move fluid along, into, out of, within (or any combination thereof) the fluid channel 112.
  • the fluid actuator 114 can be as simple as a port or as complex as a pump or diaphragm. In some embodiments, the fluid actuator 114 can be a port at the end of the fluid channel 112 (for example such as that depicted in FIG. 1) that is in fluid communication with a pump located external to the first portion. In some embodiments, the fluid actuator 114 is a port that is in fluid communication with a pump that is located on or within an external instrument that is configured to control and/or manipulate the sensor assembly. In some embodiments, the fluid actuator 114 can be a port that is in fluid communication with an entire fluidic control system.
  • Illustrative fluidic control systems can include a pump(s), diaphragm(s), valve(s), further fluid channel(s), reservoir(s), or some combination thereof.
  • at least portions of the illustrative fluidic control system can be located on or within an external instrument, the first portion of the sensor assembly, the second portion of the sensor assembly, or some combination thereof.
  • the fluid actuator 114 can include a diaphragm that is in fluid communication with some portion of a fluidic control system.
  • the first portion also includes an introducer 116.
  • the introducer 116 is on, within, or fluidly attached to the fluid channel 112 and functions to access the wells of the second portion
  • the function of the introducer 116 can also be described as being configured to obtain at least a portion of the contents of at least one well on the second portion.
  • the introducer 116 can be described as being able to both puncture sealed wells of the second portion and access and obtain at least a portion of the material in the well.
  • the introducer 116 can be actuated by an external instrument in order to access the wells. Such actuation can include movement in one or more than one dimension. For example, in the example depicted in FIG. 1, movement of the introducer 116 in the z direction could afford access to at least one well on the second portion.
  • the introducer 116 can also be configured to introduce air into a well it has accessed. This may allow the introducer 116 to more reliably obtain material from the wells. This optional function of the introducer 116 can be realized by the particular design of the tip of the introducer, by puncturing the seal to the well at two (instead of one) points simultaneously, at different times in a specified order, or by combinations thereof. In some embodiments, the introducer 116 can be similar in shape and configuration to a pipette tip.
  • the introducer 116 can also be configured to both extract material from a well of the second portion and introduce material into a well of the second portion.
  • the external instrument in some embodiments through control of a pump for example, can control whether the introducer 116 is extracting or introducing material from or into the well.
  • Introducing material into a well can allow for storage of materials, while not requiring a user to have concerns about liquids spilling out of a used sensor assembly. Introducing material into a well can also provide a method of mixing. Introducing material into a well can also provide a method of storing an intermediate composition while another step of a protocol is being carried out.
  • the first portion 110 can also include an analysis sensor 118.
  • An analysis sensor in a first portion can be any type of sensor, for example it could be an optical sensor (using for example chemiluminescence or fluorescence), an electrochemical sensor, or a resonant sensor.
  • the analysis sensor 118 can include at least one resonator sensor. Examples of such sensors can include bulk acoustic wave (BAW) sensors and thin film bulk acoustic resonator (TFBAR) sensor. Both BAW and TFBAR sensors include a piezoelectric layer, or piezoelectric substrate, and input and output transducer. Both BAW and TFBAR sensors are small sensors making the technology particularly suitable for use in handheld or portable devices.
  • BAW bulk acoustic wave
  • TFBAR thin film bulk acoustic resonator
  • the analysis sensor 118 can be within or form part of the fluidic pathway. More specifically, in some embodiments, the analysis sensor 118 can be within or form part of the fluid channel. For example, a portion of the fluidic pathway can be configured to exist within or form part of the fluidic pathway so that fluid in the fluidic pathway flows over the analysis sensor. In some embodiments, the fluid in the fluidic pathway can travel completely around the sensor, and in other embodiments, the fluid in the fluidic pathway can travel around less than all surfaces of the analysis sensor. In some embodiments, the fluid in the fluidic pathway can travel across the active region of the analysis sensor. In some embodiments, the fluid in the fluidic pathway can flow over the piezoelectric layer of the analysis sensor, which is coated with binding sites for an analyte of interest.
  • the analysis sensor can be any type of sensor.
  • the analysis sensor can be an optical sensor (for example a chemiluminescent sensor or a fluorescent sensor) or a resonant sensor, for example.
  • the analysis sensor can be a resonant sensor, such as a BAW sensor.
  • Disclosed devices include a conductivity sensor disposed within the fluidic channel.
  • the conductivity sensor can generally include at least two electrodes.
  • the electrodes are made of a material that will not be reactive with the fluid (e.g., sample, reagents, or sample and reagents in combination) that will be located within the fluidic channel.
  • the electrodes can be made of a substantially chemically inert material.
  • the electrodes can be made of gold for example.
  • the at least two electrodes can have the voltage and current characteristics configured in order to measure the resistance of the fluid within the fluidic channel.
  • the at least two electrodes can be employed with alternating current (AC), direct current (DC), or a combination thereof.
  • AC current can be utilized as it will prevent or at least minimize hydrolysis at the surface of the electrodes.
  • relatively low current are utilized in order to avoid substantial joule heating of the fluid within the fluidic channel.
  • the conductivity sensor can be in communication, for example electrical communication, with a processor that can obtain signals from the sensor and utilize the signal for various purposes. Additionally, or alternatively, the conductivity sensor can be controlled by a processor, e.g., the processor can activate the conductivity sensor at various times, for various lengths of time, or combinations thereof.
  • the conductivity sensor can be located upstream of the analysis sensor or downstream of the analysis sensor. In some embodiments, the conductivity sensor can be located upstream of the analysis sensor. In embodiments where the conductivity sensor is located upstream of the analysis sensor, the conductivity sensor can be utilized to meter sample, reagents, both sample and reagents or mixtures thereof.
  • Disclosed methods can therefore include steps of monitoring a conductivity sensor located within the fluidic channel, moving liquid (or starting the flow of liquid) within the fluidic channel, and monitoring a decrease in resistance at the conductivity channel. Various steps can optionally be carried out in addition to the three steps above depending on the purpose and/or goal of a disclosed method.
  • a system including a conductivity sensor can be utilized to meter fluids.
  • the fluid can be stopped consistently when the resistance change is detected.
  • fluid could be stopped immediately when the resistance lowers. This is accomplished by stopping the driving mechanism very shortly or even immediately after the resistance changes.
  • Disclosed methods can therefore include steps of monitoring a conductivity sensor located within the fluidic channel, starting the flow of liquid within the fluidic channel, monitoring a decrease in resistance at the conductivity sensor, and stopping the flow of the liquid in the fluidic channel.
  • pressure that has built up in the system to drive the fluid could make stopping the fluid quickly somewhat difficult. In this case, slower fluid flow would lower the pressure build up and allow the fluid to stop more quickly. The problem may worsen as the sample viscosity increases since a higher pressure is needed for an equivalent flow rate.
  • Some such disclosed methods can therefore include steps of monitoring a conductivity sensor located within the fluidic channel, starting the flow of liquid within the fluidic channel in a first direction at a first flow rate, monitoring a decrease in resistance at the conductivity channel, stopping the flow of the liquid in the fluidic channel, and reversing the flow of liquid in the fluidic channel at a second flow rate where the first flow rate is faster than the second flow rate and then stopping the flow of fluid. Methods such as this may only see modest gains in reducing the metering time.
  • a second type of method for allowing fast metering includes moving the fluid fast toward the electrodes and upon the resistance change the pump is stopped and vented. This removes the residual pressure buildup in the fluid channels quickly and allows the fluid to stop quickly.
  • Some such disclosed methods can therefore include steps of monitoring a conductivity sensor located within the fluidic channel, starting the flow of liquid within the fluidic channel in a first direction at a first flow rate, monitoring a decrease in resistance at the conductivity channel, stopping the flow of the liquid in the fluidic channel, and venting the fluidic channel.
  • a system including a conductivity sensor can be utilized to estimate the total volume of a slug of fluid in a portion of the fluidic channel.
  • a volumetric metering method such as described above (or another)
  • typically the presence of fluid is guaranteed at the meter stop (conductivity sensor, hydrophobic vent or other) and an assumption is made that fluid is continuous in the meter channel.
  • a conductivity sensor can be used to confirm that the fluid is continuous in the meter channel and estimate the total volume.
  • the metered fluid slug can be driven past the conductivity sensor and the volume of the fluid slug can be estimated by the fluid driving method used. For example a positive displacement pump can be used to drive the fluid at a specific flow rate.
  • the total time that liquid is observed on the conductivity sensor can then be used to estimate the volume of fluid based on the flow rate.
  • Some such disclosed methods can therefore include steps of monitoring a conductivity sensor located within the fluidic channel, starting the flow of liquid within the fluidic channel, determining the time that the resistance was maintained at a substantially constant value and determining the volume of fluid in at least a portion of the fluidic channel based on the time that the resistance was maintained at a substantially constant value and the flow rate of the liquid.
  • Pressure data could also be used to improve the estimate by compensating for the actual fluid flow rate when the fluid flow rate is not in steady state.
  • Some such disclosed methods can therefore include steps of monitoring a conductivity sensor located within the fluidic channel, monitoring the pressure within the fluidic channel, starting the flow of liquid within the fluidic channel, determining the time that the resistance was maintained at a substantially constant value and determining the volume of fluid in at least a portion of the fluidic channel based on the time that the resistance was maintained at a substantially constant value, the flow rate of the liquid, and the pressure within the fluidic channel.
  • Disclosed systems can also be utilized to control mixing of a sample and reagents, or any combination thereof.
  • any of the above methods of determining a volume of liquid in a fluidic channel can be combined with a step of adding the volume of liquid to a known volume of a second liquid.
  • the first liquid can be a sample and the second liquid can be a reagent
  • the first liquid can be a reagent and the second liquid can be a sample
  • the first liquid can be a reagent and the second liquid can be a reagent
  • the first liquid can be a sample and the second liquid can be a diluent for the sample, or any combination thereof.
  • reagent can be metered more than once into an already accurate/precise volume of reagent two.
  • volumetric metering methods in a fluidic device are dependent on accurate channel geometries as well as assembly tolerances. Injection molded parts produced from the same cavity are consistent enough to achieve good precision using this method. However, parts produced from multiple cavities may not be consistent enough due to tool variations.
  • reagent one is metered from an excess (e.g., a method including monitoring a conductivity sensor located within the fluidic channel, starting the flow of liquid within the fluidic channel, monitoring a decrease in resistance at the conductivity channel, and stopping the flow of the liquid in the fluidic channel).
  • reagent two is metered from an excess using the same metering method.
  • the two metered volumes are then mixed.
  • a 1 : 1 mixture is achieved with accurate and precise concentration but the final volume could be considered inaccurate. If the accuracy of the total volume of the mixture is not important to the final test result, this method avoids the multi-cavity tool problem, with the added expense of two meter steps.
  • the ratio is not limited to 1 : 1.
  • the first liquid (and/or the second liquid) could be metered multiple times (a fixed volume meter apparatus) to achieve different mixture ratios.
  • Variable volume meter methods certainly are not limited either.
  • the volume of the liquid in ratiometric methods can be estimated using a number of factors, including for example, the fluid volume in the fluidic channel using the geometry of the channel, the pressure in the channel, the flow rate of the fluid, the fluidic resistance, the time the fluid was moved, or some combination thereof.
  • Disclosed assemblies also include a second portion.
  • the second portion can include at least one well.
  • FIG. 1 depicts an illustrative second portion 120 that includes a plurality of wells 122.
  • Disclosed second portions of the assembly can include any number of wells.
  • a second portion can include at least one (1), at least three (3), or at least five (5) wells.
  • a second portion can include nine (9) wells with one being a sample well.
  • the wells can be present in any configuration, linear (as depicted in FIG. 1, circular, or otherwise).
  • the wells within a second portion can be configured to contain the same or different volumes.
  • the wells can be of a size to contain at least 10 pL.
  • the wells can be of a size to contain from 50 pL to 150 pL, for example.
  • the wells can be of a size to contain about 100 pL for example.
  • the wells can have a total volume that is more than the quantity which they are designed to hold. For example, a well can have a total volume that is 200 pL in order to house a volume of 100 pL.
  • the wells can have various configurations, including for example corners, flat bottoms, and round bottoms.
  • the wells can have various shapes, for example, they can be cylindrical, or spherical, hexagonal, or otherwise.
  • Wells within a second portion can contain various materials or can be empty. In some
  • a second portion can include at least one well that is empty.
  • a second portion can include at least one sample well.
  • the sample well can generally be empty before the assembly is used.
  • the sample well in such embodiments can be utilized to hold at least a portion of the sample transferred from a sample introduction chamber via the sample introduction pathway.
  • the sample well can include one or more than one materials, which the sample will be combined with upon introduction into the sample well.
  • Materials contained within wells can be liquid or solid. Materials contained within wells can also be referred to as reagents, diluents, wash solutions, buffer solutions, or other such terms.
  • material within a well can be a single material that is a liquid at room temperature, a solution containing more than one material, or a dispersion containing one material dispersed in another.
  • material within a well can be a solid. The material within an individual well can be independently selected with respect to materials in other wells. In some embodiments, the materials within a well are selected to carry out a particular testing protocol.
  • the second portion can also include a seal.
  • the seal functions to contain the materials within the wells.
  • the seal can be a unitary element, while in some embodiments, the seal can be made up of more than one element.
  • a single element could cover all of the wells 122.
  • each well 122 could be covered by an individual element, with all of the elements making up the seal.
  • the seal can be made of any material that can function to maintain the contents of the wells within the wells, but also allow the introducer access to the materials in the wells.
  • Illustrative materials can include, for example, a foil, such as a metallic foil which can be sealed to the second portion (or portions thereof) via an adhesive or heat sealing; plastic films; or other such materials.
  • a foil such as a metallic foil which can be sealed to the second portion (or portions thereof) via an adhesive or heat sealing; plastic films; or other such materials.
  • the seal is made of a metallic foil and covers the entirety of the second portion.
  • the second portion can also include a way of introducing a sample either directly or indirectly from a user.
  • a second portion can include an empty well, whose seal can be pierced (if it is sealed) by a portion of a disclosed assembly or a user to introduce a sample to be tested by the sensor assembly. This well can be referred to as the sample well.
  • the sample well is not covered by the seal.
  • the sample can be added to a sample well via, for example a sample introduction pathway.
  • the devices, systems, and methods described herein may be employed to detect a target analyte in a sample.
  • the devices may find use in numerous chemical, environmental, food safety, or medial applications.
  • a sample to be tested may be, or may be derived from blood, serum, plasma, cerebrospinal fluid, saliva, urine, and the like.
  • Other test compositions that are not fluid compositions may be dissolved or suspended in an appropriate solution or solvent for analysis.
  • Non-limiting examples of target analytes include nucleic acids, proteins, peptides, antibodies, enzymes, carbohydrates, chemical compounds, or infectious species such as bacteria, fungi, protozoa, viruses, pesticides and the like.
  • the target analyte is capable of binding more than one molecular recognition component.

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Abstract

L'invention concerne des dispositifs qui comprennent une première partie, la première partie comprenant au moins un canal de fluide ; un actionneur de fluide ; un capteur d'analyse prévu à l'intérieur du canal de fluide ; un capteur de conductivité prévu à l'intérieur du canal de fluide ; et un introducteur ; une seconde partie, la seconde partie comprenant : au moins un puits, le puits contenant au moins un matériau, la première ou la seconde partie étant mobile par rapport à l'autre, l'introducteur étant conçu pour obtenir au moins une partie du matériau à partir du ou des puits et le distribuer au canal de fluide, et l'actionneur de fluide étant conçu pour déplacer au moins une partie du matériau dans le canal de fluide.
PCT/US2019/039470 2018-07-06 2019-06-27 Canaux fluidiques comprenant un capteur de conductivité et leurs procédés d'utilisation WO2020009897A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201980045635.4A CN112469998B (zh) 2018-07-06 2019-06-27 包含电导率传感器的流体通道及其使用方法
EP19831197.9A EP3818370A4 (fr) 2018-07-06 2019-06-27 Canaux fluidiques comprenant un capteur de conductivité et leurs procédés d'utilisation
JP2021500212A JP7518060B2 (ja) 2018-07-06 2019-06-27 伝導度センサを含む流体チャネルおよびその使用方法

Applications Claiming Priority (10)

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US201862694594P 2018-07-06 2018-07-06
US201862694621P 2018-07-06 2018-07-06
US201862694599P 2018-07-06 2018-07-06
US62/694,594 2018-07-06
US62/694,599 2018-07-06
US62/694,621 2018-07-06
US16/120,224 US11344878B2 (en) 2018-07-06 2018-09-01 Fluidic channels including conductivity sensor and methods of use thereof
US16/120,225 2018-09-01
US16/120,225 US11541385B2 (en) 2018-07-06 2018-09-01 Methods of measuring hematocrit in fluidic channels including conductivity sensor
US16/120,224 2018-09-01

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PCT/US2019/039470 WO2020009897A1 (fr) 2018-07-06 2019-06-27 Canaux fluidiques comprenant un capteur de conductivité et leurs procédés d'utilisation

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