WO2019008461A1

WO2019008461A1 - Apparatus and method for obtaining at least 2d analyte information and detection element designed therefor

Info

Publication number: WO2019008461A1
Application number: PCT/IB2018/054575
Authority: WO
Inventors: Daniela OBERMAIER; Gregor Liebsch; Robert J MEIER; Achim Stangelmayer
Original assignee: Presens Precision Sensing Gmbh
Priority date: 2017-07-06
Filing date: 2018-06-21
Publication date: 2019-01-10

Abstract

Disclosed are an apparatus (1), a method and a detection element (10) for obtaining at least 2D analyte information. A camera (2) is provided with a 2-dimensional sensor chip (4) and an illumination device (6) for producing illumination light (35). At least one detection element (10) is provided with a sensor film (18). The 2-dimensional sensor film (18) is illuminated via the detection element (10). The sensor film (18) is provided in the region of the second end (12) of the detection element (10). A camera (2) images the light from the sensor film (18) onto a sensor chip (4).

Description

Apparatus and method for obtaining at least 2D analysis information and detection element designed therefor

The present invention relates to a device for obtaining at least 2D analyte information of a sample.

Furthermore, the invention relates to a method for obtaining at least 2D analyte information of a sample, wherein the sample can be designed to be versatile.

Furthermore, the invention relates to a detection element with which it is possible to obtain at least 2D analyte information within a sample by means of a single measurement. In addition, by special design of the

Measurement method also a time-resolved information about the 3D analyte distribution in the sample can be obtained (advantage).

German patent application DE 101 01 576 A1 discloses an optical sensor for determining at least one parameter in a sample. The optical sensor includes a short-decay indicia responsive to the parameter and a long-decay non-responsive reference material for sensing a measurement signal indicative of the parameter to be determined. The indicator and reference materials are immobilized on a common support. The sample facing layer of the

Indicator material and the reference material is covered by a layer that allows contact between the indicator material and the sample, but for the light used in the excitation of the indicator and reference material

Essentially impermeable.

German laid-open specification DE 198 38 085 A1 discloses a method for examining a floor. The method is characterized by the fact that electromagnetic radiation at different locations in the measuring range coupled and / or decoupled from the measuring range at various points.

US Patent Application US 201 1/0086418 A1 discloses oxygen sensors provided in a multiwell plate. The oxygen sensors are illuminated with visible light. The emission from the oxygen sensors is recorded with a camera. The images are analyzed with appropriate software.

US Pat. No. 5,604,582 discloses an apparatus for determining depth profiles in water-covered sediments. The device does not provide analyte information. Only a pure image acquisition in the z-direction is obtained by deflection over a mirror.

According to the prior art, for recording depth profiles in animal and plant tissues, soils, sediments, interfaces or cell cultures

Microsensors (optochemical and potentiometric) provided. Over a

Measurement control, it is possible to use these microsensors in the depth of

introduce the analyzing sample. The result is thus one

Analytical composition or concentration in the depth of the sample. The point measurements are carried out by means of thin glass fibers. The disadvantage of this method is that it is time-consuming and uses very fragile sensors. An example of an optode is described in US Pat. No. 5,273,716. In order to monitor the oxygen content and the pH in cell cultures, soils or sediments, according to the prior art a sensor can be arranged on the bottom of a well. The sensors are point sensors, also called "dots", and purely optochemical.With the method proposed here or with the proposed device, it is possible to perform point measurements, however, no information is obtained on the two-dimensional distribution or on gradient profiles. A device for determining the distribution of an analyte in a sample according to the prior art comprises a sensor film which is attached to a wall of a

Container is glued. A camera, which also includes a lighting device, illuminates the sensor film through the wall of the vessel. The light coming back from the sensor foil is detected by a sensor chip of the camera. With the apparatus of the prior art, it is possible to measure the distribution of oxygen, the pH and the carbon dioxide concentration. This is e.g. in European Patent Application EP 1 040 788 A1.

The application of a multi-fiber optode (MuFO) can be found in the link: www.mpi-bremen.de/en/Multi-Fiber-Qptode-MuFQ.html. The MuFO only allows a combination of point measurements to capture information in the z-direction. A continuous 2D information can not be achieved with it.

The invention has for its object to provide a device for obtaining at least 2D property information that can be performed or used in a quick and easy way. By means of the device, the

Users perform the measurement without any equipment and thus

Gain analyte information regarding the multi-dimensional distribution of an analyte in a sample. This object is achieved by a device for obtaining at least 2D property information according to the features of claim 1.

The invention has for its object to provide a method for obtaining 2D analty information that can be performed or used in a quick and easy manner, so that by means of the measurement without effort for the user at least 2D analyte information for the distribution of an analyte in a sample can be obtained. This object is achieved by a method for obtaining at least 2D property information according to the features of claim 9.

It is a further object of the invention to provide a detection element for obtaining at least 2D analyte information suitable for use in rapidly and easily obtaining at least 2D analyte information regarding the distribution of an analyte in a sample.

This object is achieved by a detection element for obtaining at least 2D analyte information comprising the features of claim 14.

A possible embodiment of the device for obtaining at least 2D analyte information comprises a camera with a two-dimensional sensor chip. Likewise, a lighting device for generating an illumination light is provided. Furthermore, at least one detection element is provided which has a first end and a second end. A second surface is formed in the region of a second end of the at least one detection element and provided with a 2-dimensional sensor film. Furthermore, at least one container is provided with a sample, wherein the detection element can be accommodated in the container. The 2-dimensional sensor film is completely covered by the sample. An evaluation system is communicatively connected to the sensor chip of the camera, which takes a picture of the 2-dimensional sensor film. If the detection element is inserted into the container provided for it, one differentiates between two possible embodiments. According to the first

Embodiment remains open even after the insertion of the detection element in the container upwards, so that a gas exchange can take place from the interior of the container with the environment. According to the second

Embodiment is after insertion of the detection element in the container, this closed at the top. This prevents a gas exchange from the interior of the container with the environment. The device has the advantage that with suitable alignment of the sensor film from the recorded 2-dimensional image of the sensor film information about a spatial distribution of the analyte in the sample can be obtained. Similarly, one can obtain information about the temporal evolution of the analytes in the sample.

According to a possible embodiment, the 2-dimensional sensor film can be mounted on a holding means in the region of the second end of the detection element

be upset.

The holding means may according to one embodiment be a ramp which

is inclined by an angle relative to a horizontal.

According to one embodiment of the detection element is a solid

Light guide disposed in the detection element between the first end and the second end. The light guide element has at the first end a first surface for coupling illumination light and for coupling out detection light. At the second end, a second surface is formed, which is provided with the 2-dimensional sensor film.

According to a further possible embodiment, the second surface may be aligned at the second end of the light-guiding element of the detection element parallel to a horizontal. According to another embodiment, the second surface may be aligned at the second end of the light-guiding element of the detection element at an angle to a horizontal.

The camera is arranged together with the sensor chip such that the 2-dimensional sensor film defines an image window on the sensor chip in the region of the second end of the at least one detection element. This has the advantage that only the first surface at the first end of the

Light-conducting element of the at least one detection element emerging

Detection light is detected. According to one embodiment of the device according to the invention, the at least one container is a well. Several wells are arranged in a corrugated plate, wherein one of the detector elements is inserted in at least one of the wells. An analyte consumption does not take place during the measurement. This allows you to monitor for a long time hypoxic states without them at

The same applies to pH and CO2, and a computer is provided for communicating with the sensor chip of the camera, and an evaluation unit directly connected to the camera's sensor chip, such as a mobile phone, can be used or

Smart camera, be provided. The term computer means any device with which an evaluation can be carried out. Suitable devices are e.g. Laptop, PC, tablet, mobile phone, etc.

The method according to the invention for obtaining at least 2D analyte information initially comprises the step that at least one

Detection element is introduced into a sample having at least one analyte to be detected, wherein the at least one detection element has a first end, a second end and a light element, which extends from the first end to the second end. The second end of the light guide carries on the two-dimensional surface or surface structure (not necessarily is a

continuous ramp required) a sensor film. The area or

Surface structure may have steps, corners or edges (ie several

different two-dimensional surfaces). Also, the size, shape and topography of the sensor may vary to detect at least one of the analytes present in the sample. After that at least one

Detection element is inserted or inserted at least into the sample, the at least one detection element can be illuminated so that illumination light passes over a first surface in the region of the first end of the detection element to the provided on the second surface of the second end sensor film. The detection light returning from the sensor film via the light-guiding element arrives at a sensor chip of a camera. The detection light, which emerges via the second area in the region of the first end of the light-guiding element, is detected by the sensor chip. From the detection one thus obtains a representation of the at least two-dimensional distribution of an analyte within a sample. This can be achieved without the use of technically complex SD acquisition methods, such as confocal imaging systems.

With the method according to the invention, it is thus possible

Depth profile information on the distribution of analytes in animal and

Plant tissue, in soils, sediments or cell cultures. According to one embodiment of the invention, the at least one container may be a well, wherein a plurality of wells are arranged in a corrugated plate. In at least one of the wells, one of the detector elements is used. The corrugated plate is illuminated as a whole. With the camera, the corrugated plate is imaged with the at least one detector element on the sensor chip, wherein an image area is defined for each 2-dimensional sensor film on the sensor chip. The data from the respective image area are fed to an evaluation unit,

The detection element according to the invention for obtaining at least 2D analyte information is designed accordingly. The detection element comprises a first end and a second end. A 2-dimensional sensor film is provided in the region of the second end of the detection element.

The 2-dimensional sensor film is applied to a holding means in the region of the second end of the detection element. The detection element has an opening at the first end. According to one embodiment of the

Detection element, the holding means is a ramp opposite to a

Horizontal is inclined at an angle.

Likewise, the detection element may comprise a solid light-guiding element which is arranged between the first end and the second end. The Light guide element has at the first end a first surface for coupling

Illumination light and designed for coupling out detection light. At the second end, a second surface is formed, which is provided with the 2-dimensional sensor film. According to one embodiment, the second surface is aligned with the 2-dimensional sensor film parallel to a horizontal

According to another embodiment, the second surface is aligned with the 2-dimensional sensor film at an angle to a horizontal.

In the region of the second end, the second surface has a sensor film or has glued them. The sensor film is capable of micrometer resolution the

Analyte differences and to translate into a non-homogeneous areal light signal, wherein the "non-homogeneity" 1: 1 reflects a present analyte gradient or an analyte distribution.The second area may be in the region of the second end at least partially to a vertical of the two-dimensional Areas of the first end to be inclined at an angle.

A key advantage of the present invention is that you do not have to move the detection element for profiling. This is exactly the same as with the microsensors of the prior art. It also follows that there is no puncture channel, which is mechanically changed in the measurement and because of the non existing due to the invention movement of the detection element, there is also no mixing of the sample during the measurement process. In addition, the invention enables a "projection of 3D information onto a 2D detector", thereby achieving a combination with the unique advantages of the opto-chemical sensors .. Embodiments of the present invention will be more apparent from the following detailed description of the invention and its advantages The size relationships in the figures do not always correspond to the actual size ratios, since some shapes simplified and other forms are shown enlarged in relation to other elements for ease of illustration. Showing:

FIG. 1 shows a schematic representation of a cell culture provided in a well of a microtiter plate and the expected cell culture thereof

Oxygen gradients in the Z direction;

Figure 2 is a schematic representation of the use of a cell crown and the resulting distribution of oxygen above and below the cell crown;

Figure 3 is a perspective view of a first part of a special

Embodiment of a container for the production of

Analyte gradients in the Z direction;

Figure 4 is a perspective view of a ramp, which at the bottom of the in FIG

3 part of a container can be attached;

FIG. 5 is a graph of the distribution of oxygen (answer of the

Sensor film) as a function of time and in a location at the bottom of the

Wells and at the position of the membrane on the cell crown;

Figure 6 is a schematic representation of the oxygen gradient in the Z direction after the cell crown has been inserted;

Figure 7 is a schematic representation of the oxygen gradient in the Z direction in

Well, after a past period of time;

Figure 8 is a plan view of a microtiter plate, the 4x6 in a regular

Raster arranged wells and wherein in the wells partly different embodiments of the detection element are used; Figure 9 is a plan view of a possible embodiment of the

Detection element;

Figure 10 is a side view of the detection element shown in Figure 9;

Figure 1 1 is a plan view of another possible embodiment of the

Detection element;

Figure 12 is a side view of the detection element shown in Figure 1 1;

Figure 13 is a schematic representation of an apparatus for detecting the

Z-direction analyte concentration on a variety of samples housed in multiple wells of a microtiter plate; Figure 14 is a schematic plan view of a microtiter plate, wherein the second

Row of microtiter plate are filled with detection elements according to an embodiment of the invention; and

Figure 15 is a schematic plan view of the sensor chip, which is used in the camera, wherein according to the position of the detection elements used in Figure 14 in the microtiter plate for detection on the sensor chip corresponding image windows are designed by software.

Identical or equivalent elements of the invention will be identical

Reference numeral used. Furthermore, for the sake of clarity, only reference symbols are shown in the individual figures, which are required for the description of the respective figure.

FIG. 1 shows a culture of cells 29 deposited on the bottom 23 of a well 22. With the invention it is possible to determine the oxygen concentration (analyte) not only in one plane (two-dimensional), but also in the associated Z-direction Z (height) of the sample 17. The Z gradient of oxygen (analyte) can thus be determined. The Wells 22 are usually in Microtiter plates or corrugated plates 26 formed. Advantageously, the microtiter plates or corrugated plates 26 are formed black, so that illumination light is filtered away and thus no interference signals for recording the

Fluorescence signals result. Crosstalk of the fluorescence signals from one well 22 to adjacent wells is thus prevented.

FIG. 2 shows the use of cell crowns 27, one cell crown 27 being inserted into one well 22 in each case. By means of the cell crown 27, the cells 29 do not form on the bottom 23 of the respective well 22. The cells 29 are thus accessible from all sides through the sample 17. This also makes it possible to determine the Z gradient of the oxygen distribution in the Z direction Z within the well.

FIG. 3 shows a first part 31 of an embodiment of a detection element 10 which, together with a second part 32 shown in FIG

Container (Well) 22 can be used. It is for a professional

of course, that the container 22 need not be limited to the size of a well for a microtiter plate 26 alone. The first part 31 is cylindrical and has an inner diameter Di, which is smaller than the diameter Dz (see Figure 2) of a cell crown (not shown here). With the second part 32, the first part 31 can be closed downwards. The second part 32 forms the bottom 23 of the detection element 10. On the bottom 23 is the cell crown 27 (see Figure 2).

As can be seen from FIG. 4, the second part 32 has a ramp 24 which can be inserted at the bottom 23 of the detection element 10. The ramp 24 serves as a holding means 13 and carries the required for the determination of the Z gradient of the analyte sensor film 18. The cell crown 27 (not shown here) is on formations 25 of the bottom 23. The sensor film 18 itself is under the

Cell crown 27 is provided. With the embodiment described in FIGS. 3 and 4, it is thus possible to determine three-dimensional information regarding the distribution of a specific substance (analyte) in the sample. The information becomes taken with a camera 2 and evaluated via a sensor chip 4 (see Fig.13).

The first part 31 of the detection element 10 has an opening 38, through which the camera 2 looks at the sensor film 18 in order to record an image of the light returning from the sensor film 18 (fluorescent light). The format and the size of the sensor film 18 or the inclination of the ramp 24 can be individually adapted to the shape and size of the well or container 22. Depending on the needs of the user, the ramps 24 of the second part 32 of the detection element 10 can be printed accordingly with a 3D printer (not shown) or

getting produced.

FIG. 5 shows the evaluation of the oxygen distribution (analytes) on the bottom 23 of a well 22 and, in comparison, on the membrane of cells 29 formed by the cell crown 27 as a function of time. It can be clearly seen that with

increasing the time the oxygen concentration increases both at the bottom 23, as well as at the position of the cells 29 on the cell crown 27 of the membrane. The oxygen decreases. In the graph, the response of the sensor film 18 is shown in [a.u.]. The higher the value (oxygen concentration) on the ordinate 52, the lower the oxygen concentration. The increase in the oxygen concentration in the region of the membrane of cells 29 is significantly greater than the rise at the bottom 23 of the well 22.

Figure 6 shows a schematic representation of the distribution of

Oxygen concentration Z direction Z in a well 22, in which a cell crown 27 is inserted. FIG. 7 shows the development of the distribution of the oxygen over time, wherein the ramp 24 with the sensor film (not shown here) has already been in the sample 22 (nutrient solution) in the well 22 for some time. To the

Cells 29 on the cell crown 27 develop, as can be seen from FIG. 7, with time a high oxygen concentration in the Z direction Z. Figure 8 shows a plan view of a corrugated plate 26 in which several different embodiments of a detection element 10 are used, which find use in the present invention. Depending on the type of detection (2- dinemsionale detection or 3-dimensional detection) of the user, the detection elements 10 can be selected and distributed in the microtiter plate 26. For the measurement, the corrugated plate 26 is aligned in the X-coordinate direction X and in the Y-coordinate direction Y. Each used in the corrugated sheet 26

Detection element 10 is open at the top, so that a gas exchange with the environment can take place. It is obvious to a person skilled in the art that according to another possible embodiment of the invention according to the

Inserting the detection element 10 in the corrugated plate 26, a closure to the environment takes place. The gas exchange with the environment is not possible or prevented.

FIG. 9 shows a plan view of a further possible embodiment of the detection element 10. FIG. 10 shows a side view of the detection element 10 of FIG. 9. In the detection element 10 extends centrally a light guide element 14, with which the illumination light 35 (see FIG first end 1 1 can be transported to a second end 12 of the light-guiding element 14. The second end 12 sits in the sample (not shown here) and carries a sensor film 18. At the first end 1 1, the detection element 10 has a ring 28th

educated. The light guide 14 is enclosed by the ring 28 and has a substantially rectangular cross-sectional shape. From the side view of

Detection element 10 can be seen that extends the light-guiding element 14 in the Z direction Z. A first side 14i of the light element 14 is shorter than a second side 142 of the light guide element 14. This results in a ramp 24. By means of this ramp 24 it is thus possible to provide different information

to obtain different Z positions within the sample. The light-guiding element 14 is designed substantially as a trapezoidal quadrangle, wherein at the first end 1 1, a first surface 15 is formed and in the region of the second end 12, the light-guiding element 14 Ramp 24 and second surface 16 which extends at an angle to the Z-direction Z. The angle can not be equal to 90 °.

FIG. 11 shows a plan view of a further embodiment of the invention

Detection element 10. FIG. 12 shows a side view of the detection element on FIG. In the detection element 10 also extends centrally

Light guide 14, with which the illumination light 35 (see FIG. 13) can be transported from a first end 1 1 to a second end 12 of the light guide 14. The second end 12 is surrounded by the sample (not shown here) and carries a sensor film 18. Here is also a ring 28 at the first end 1 1 of the detection element 10 is provided. Centrally in the detection element 10 extends the light-guiding element 14, which is held by a plurality of carriers 30 in the central position in the detection element 10. As also mentioned in the description of FIG. 10, a sensor film 18 is glued to the second end of the light-guiding element 14. The light-guiding element 14 extends substantially in the Z-direction Z and may be conical. Thus, it is possible that space is also provided on the lateral wall 19 of the light-guiding element 14 in order to attach a sensor foil 18. This gives a 3D analyte information of the sample.

The embodiments of the illustrated in Figures 9 to 12

Detection element 10 should not be construed as limiting the invention. It is obvious to a person skilled in the art that different geometries can be formed for the second surfaces 16 (ramps) of the light-guiding elements 14, on which the sensor film 18 is applied. It is also conceivable that the second surface 16 is formed for the sensor film 18 as a continuously differentiable surface. With the embodiment of the device according to the invention or the detection elements 10 used for the device, it is possible to obtain statements of a multiplicity of systems by a two-dimensional image of optochemical sensor material which is applied to 3-D structures. Beyond the first surfaces 15 of the embodiments

Illumination light 35 on and detection light 36 coupled out. With the invention thus obtained a projection of a three-dimensional

Information in a two-dimensional plane. In addition to the two-dimensional information in the XY plane, one also obtains a depth profile in the Z direction Z of the sample 17. In addition, one can measure the information with several analytes simultaneously. Furthermore, one can determine point measurements and additionally the time course of the change of the analyte. The duration of a recording for

Obtaining the information corresponds to that of a conventional photo shoot.

Thus, it is also possible to record videos about the change in the distribution of analytes within a sample in three dimensions. Figure 13 shows a schematic structure of the device 1, according to a

Embodiment of the invention for obtaining 3D analyte information within a sample 17. Here are several of the invention

Detection elements 10 are inserted into a respective well 22 of a corrugated plate 26. With a camera 2, which comprises a sensor chip 4 and a lighting device 6, the entire corrugated plate 26 is illuminated with illumination light 35. Furthermore, the camera 2 has a suitable optical system (not shown here) with which the detection light 36 coming back from the detection elements 10 is imaged on the sensor chip 4. The information obtained by means of the sensor chip 4 is transferred to a computer 8 for evaluation or further processing. The camera 2 defines an optical axis 7, which is aligned substantially perpendicular to the corrugated plate 26.

Although in the embodiments shown in FIGS. 9 to 13, the detection element 10 inserted in the container or well 22 allows gas exchange with the environment, this should not be construed as limiting the invention. As already mentioned above, an embodiment is possible which prevents gas exchange with the environment

FIG. 14 shows a plan view of the sensor chip 4 of the camera 2 according to an embodiment. The entire corrugated plate 26 is shown here in dashed lines and is, in this embodiment, by the camera 2 and its optics as a whole on the Sensor chip 4 shown. In the illustration shown in Figure 13 are

For example, six detection elements 10 arranged in series in the corrugated plate 26. As can be seen from the illustration of this figure, here a corrugated plate 26 having four rows and six columns is used. Thus, 24 wells 22 result in the corrugated plate 26th

For reading out the information of the detection elements 10, a plurality of image windows 20 are defined on the sensor chip 4 of the camera 2. The image windows 20 always correspond in shape and arrangement of the light guide elements 14 at the first end 1 1 (see FIG. 14) of the detection element 10. Although in the representation shown in Figure 15, a series arrangement of

Image window 20 is shown, this should not be construed as a limitation of the invention. In terms of software, any desired orientation of the shape and arrangement of the image window 20 can be selected on the sensor chip 4 of the camera 2. Likewise, the application of the detection elements 10 is not limited to microtiter plates or corrugated plates 26. As already mentioned, these detection elements 10 according to the invention can also be introduced into the soil or into sediments in order thus to obtain a three-dimensional distribution of the analytes within the soil or in the sediments.

The invention has been described in terms of preferred embodiments, which are in no way to be construed as limiting the invention. However, it will be understood by those skilled in the art that changes and modifications may be made without departing from the scope of the following claims. Ub.Ub.i.U1 ö

- 17 -

LIST OF REFERENCE NUMBERS

1 device

2 camera

4 sensor chip

6 lighting device

7 optical axis

8 computers

10 detection element

1 1 first end

12 second end

13 holding means

14 light guide

14i first page

14 ₂ second page

15 first area

16 second area

17 sample

18 sensor film

19 lateral wall

20 picture windows

22 container, well

23 floor

24 ramp

25 shape

26 well plate, microtiter plate

27 cell crown

28 ring

29 cells

30 carriers UÖ.UÖ .-. U1 Ö

- 18 -

31 first part

32 second part

35 illumination light

36 detection light

38 opening

52 ordinates

Di inner diameter

Dz diameter cell crown

H horizontal

X X coordinate direction

Y Y coordinate direction

Z Z coordinate direction

Claims

claims

Device (1) for obtaining at least 2D analyte information, comprising:

• A camera (2) with a 2-dimensional sensor chip (4), which is arranged opposite a first end (1 1) of at least one detection element (10);

A lighting device (6) for generating an illumination light;

A second surface (16) in the region of a second end (12) of the

at least one detection element (10) provided with a 2-dimensional sensor film (18);

• at least one container (22) with a sample (17) containing the

Detection element (10) receives, wherein the 2-dimensional sensor film (18) is completely covered by the analyte; and

A computer (8) communicatively connected to the sensor chip (4) of the camera (2), the sensor chip (4) receiving an image of the 2-dimensional sensor film (18).

Device (1) according to claim 1, wherein the 2-dimensional sensor film (18) on a holding means (13) in the region of the second end (12) of the

Detection element (10) is applied.

Device (1) according to claim 2, wherein the holding means (13) is a ramp (24) which is inclined relative to a horizontal (H) by an angle (a).

Device (1) according to claim 1, wherein a solid light guide element (14) in the detection element (10) between the first end (1 1) and the second end (12) is arranged, wherein the light guide element (14) at the first end (1 1 ) a first surface (15) for coupling of illumination light (35) and to

Coupling of detection light (36) and at the second end (12) has a second surface (16) which is provided with the 2-dimensional sensor film (18).

5. Apparatus according to claim 4, wherein the second surface (16) at the second end (12) of the light-guiding element (14) of the detection element (10) is aligned parallel to a horizontal (H).

6. Apparatus according to claim 4, wherein the second 2-dimensional surface (16) at the second end (12) of the light-guiding element (14) of the detection element (10) at an angle (oc) to a horizontal (H) is aligned.

7. Device according to one of the preceding claims, wherein the camera (2) with the sensor chip (4) is arranged such that the 2-dimensional

Sensor film (18) in the region of the second end (12) of the at least one detection element (10) an image window (20) on the sensor chip (4) defined so that only the first surface (15) at the first end (1 1) of the

Light-guiding element (14) of the at least one detection element (10) emerging detection light (36) can be detected.

8. Device according to one of the preceding claims, wherein the at least one container is a well (22) and a plurality of wells (22) in a corrugated plate (26) are arranged, and wherein in at least one of the wells (22) one of the detection elements (10 ) is used.

9. A method for obtaining at least 2D analyte information, comprising the following steps:

• introducing at least one detection element (10) into a container (22) with an analyte, wherein the detection element (10) has a first end

(1 1) and a second end (12), and in the region of the second end

(12) a 2-dimensional sensor film (18) is provided, which is surrounded by the analyte to be detected;

Irradiating illumination light (35) onto the 2-dimensional sensor film (18) of the at least one detection element (10), and

• Detecting with a sensor chip (4) of a camera (2) of the camera

at least one 2-dimensional sensor film (18) due to the reaction with the at least one analyte of the sample coming back Detection light (36), wherein on the sensor chip (4) of the camera (2) a corresponding image window (20) is defined.

10. The method according to claim 9, wherein the 2-dimensional sensor film (18) on a holding means (13) in the region of the second end (12) of the

Detection element (10) is applied and the 2-dimensional sensor film (18) is illuminated through the analyte and the detection light (36) from the 2-dimensional sensor film (18) passes through the analyte on the sensor chip (4) of the camera (2) ,

1 1. The method of claim 9, wherein the 2-dimensional sensor film (18) on a second 2-dimensional surface (16) of a solid Lichtleitelements (14) is arranged in the detection element (10) between the first end (1 1) and the second End (12) is provided, wherein the illumination light is coupled via a first surface (15) of the light guide element (14) passes over the second surface (16) to the 2-dimensional sensor film (18) and over the first surface (15) as Detection light (36) is coupled out and through the

Camera (2) on the 2-dimensional sensor chip (4) is displayed.

12. The method according to any one of the preceding claims 9 - 1 1, wherein the

at least one container is a well (22) and a plurality of wells (22) in a corrugated plate (26) are arranged, and wherein in at least one of the wells (22) one of the detector elements (10) is used.

13. The method of claim 12, wherein the corrugated plate (26) illuminates as a whole and with the camera (2) the corrugated plate (26) with the at least one

Detector element (10) is imaged on the sensor chip (4), wherein for each 2-dimensional sensor film (18) on the sensor chip (4) an image area is set and the data from the respective image area are fed to an evaluation unit.

14. A detection element (10) for obtaining at least 2D analyte information, wherein the detection element (10) comprises:

• a first end (11) and a second end (12); and A 2-dimensional sensor film (18), which is provided in the region of the second end (12) of the detection element (10).

15. Detection element (10) according to claim 14, wherein the 2-dimensional sensor film (18) on a holding means (13) in the region of the second end (12) of the

Detection element (10) is applied and the detection element (10) at the first end (1 1) has formed an opening (38).

16. detection element (10) according to claim 15, wherein the holding means (13) a

Ramp (24) which is inclined relative to a horizontal (H) by an angle (oc). 17. Detection element (10) according to claim 14, wherein a solid light guide element (14) in the detection element (10) between the first end (1 1) and the second end (12) is arranged, wherein the light guide element (14) at the first end ( 1 1) a first surface (15) for coupling illumination light (35) and for coupling out detection light (36) and at the second end (12) has a second 2-dimensional surface (16) connected to the 2-dimensional sensor film (18 ) is provided.

18. detection element (10) according to claim 14, wherein the second surface (16) with the 2-dimensional sensor film (18) is aligned parallel to a horizontal (H). 19. detection element (10) according to claim 14, wherein the second surface (16) with the 2-dimensional sensor film (18) at an angle (oc) to a horizontal (H) is aligned.