WO2016082558A1 - Laser nanometer-scale optical diagnostic device - Google Patents

Abstract

A laser nanometer-scale optical diagnostic device, characterized by comprising: a darkened treatment room (101); a carrying platform (102); a first laser (103) emitting a laser enabling a nanometer-scale photosensitive agent injected in the body of an object of treatment to emit light; a charge coupling image sensing device (CCD) (104) disposed in the darkened treatment room (101); an optical filter (105) disposed between the object of treatment and the CCD (104); an image processor (106) for processing an image captured by the CCD (104), and locating a light concentration region in the body of the object of treatment; a central controller (107) for locating the light concentration region in the body of the object of treatment as a tumor area, and controlling a rotating platform (108) to rotate, the rotating platform (108) being connected to a second laser (109), and adjusting an emitting direction of the second laser (109) via rotation; the second laser (109) disposed on the rotating platform (108), and emitting a laser to enable the nanometer-scale photosensitive agent to exhibit the effects of photo-thermal therapy and/or photodynamic treatment. The device monitors in real time the metabolization, distribution and concentration of the nanometer-scale photosensitive agent, accurately locates a tumor and realizes a noninvasive tumor treatment for an object of treatment.

Description

Laser nano optical diagnosis and treatment equipment

cross reference

This application claims that the filing date is November 26, 2014, the application number is 201410693311.0, and the invention title is “a laser nano-optical diagnosis and treatment equipment” priority of the Chinese invention patent.

Technical field

The present invention relates to the field of physical diagnosis and treatment technology, and in particular to a laser nano-optical diagnosis and treatment device.

Background technique

Traditional surgical resection, chemotherapy, radiotherapy or biological therapy have made remarkable achievements in cancer treatment, but its toxic side effects and multi-drug resistance are still difficult to overcome. In recent years, the photothermal and photodynamic therapy of nano-materials that penetrate the skin by near-infrared light has been increasingly favored because of its non-invasive, non-toxic, targeted, and efficient advantages.

Photothermal therapy refers to the use of the thermal effects of various pyrogens to heat the tumor area or whole body to the temperature of effective treatment, and maintain a certain time, using the difference in temperature tolerance between normal tissue and tumor tissue, to achieve both killing Tumor cells do not damage the treatment of normal tissues.

Photodynamic therapy refers to a method in which a photosensitizer is used to cause a functional or morphological change of an organism cell or a biomolecule under the action of light to achieve a therapeutic effect. Photodynamic therapy is another new research that is completely different from surgery, radiotherapy, chemotherapy and immunotherapy. It has become one of the most active research fields in the world of cancer prevention and treatment.

A large number of experiments have been carried out in photothermal and photodynamic therapy at home and abroad; at the same time, the emerging optical imaging technology of living animals has made a revolutionary leap, which has enabled the requirements of medical ethics in animal experiments, real-time optical imaging, Image-guided therapy has shown unique advantages in cancer therapy, but there is currently no tumor diagnostic equipment that integrates photothermal/photodynamic therapy with in vivo imaging.

Summary of the invention

The main object of the embodiments of the present invention is to provide a laser nano-optical diagnosis and treatment device to provide a diagnosis and treatment device that integrates photothermal therapy technology, photodynamic therapy technology and living body imaging technology.

In order to achieve the above object, an embodiment of the present invention provides a laser nano-optical diagnosis and treatment device, including:

Treating darkrooms;

a carrying platform disposed in the treatment dark room for carrying a therapeutic object injected with a nano photosensitizer in vivo;

a first laser disposed in the treatment dark chamber for emitting laser light to illuminate the treatment object, causing the nano photosensitizer injected into the body of the treatment object to emit light;

a charge coupled image sensor CCD disposed in the treatment darkroom;

a filter disposed between the treatment target and the CCD for filtering light of a set spectrum to transmit light emitted by the nano photosensitizer;

The CCD is configured to photograph the treatment object through the filter;

An image processor, connected to the CCD, for acquiring an image captured by the CCD, and performing image processing on the image to locate an area enriched in the body of the treatment object;

a rotary table disposed in the treatment darkroom;

a second laser mounted on the rotating table;

The rotating table adjusts a transmitting direction of the second laser by rotating;

a central controller that connects the image processor and the rotating table, positions an area in which the illumination object is enriched in the treatment object as a tumor area, and controls rotation of the rotating table to adjust an emission direction alignment of the second laser Tumor area;

The second laser is configured to emit laser light to illuminate the tumor region, so that the nano photosensitizer exerts photothermotherapy and/or photodynamic therapy;

The central controller is further connected to the first laser and the second laser for controlling the first laser and the second laser to be turned on or off.

By means of the above technical solution, the laser nano-optical diagnosis and treatment device provided by the invention emits laser to inject nano-photosensitive agent injected into the treatment object, and monitors the metabolism, distribution and enrichment of the nano photosensitizer in real time, thereby accurately positioning the tumor. The position is achieved by non-invasive and non-invasive tumor treatment of the treated subject by emitting laser light to the nano-photosensitive agent in the tumor region to perform photothermal therapy or photodynamic therapy.

DRAWINGS

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only the present invention. Some For the embodiments, those skilled in the art can obtain other drawings according to the drawings without any creative work.

1 is a schematic structural view of a laser nano-optical diagnosis and treatment device provided by the present invention;

2 is a schematic structural view of a laser nano-optical diagnosis and treatment device according to Embodiment 1 of the present invention.

detailed description

The technical solutions in the embodiments of the present invention are clearly and completely described in the following with reference to the accompanying drawings in the embodiments of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, but not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative efforts are within the scope of the present invention.

It should be noted that the nano photosensitizer used in the present invention is a biocompatible macromolecule such as phospholipid, phospholipid polymer, polypolysaccharide, polypeptide, albumin, etc., and surface-modified folic acid, Her2 ligand, etc. Photosensitive agents such as phthalocyanine green, IR780, porphyrin and nano gold can prepare degradable nano photosensitizers, which can achieve targeted recognition and enrichment of tumors, and have the advantages of good fluorescence stability and low toxicity.

The following is a preparation method of a nano photosensitizer used in the present invention: dissolving phthalocyanine green, soybean lecithin and distearyl phosphatidylethanolamine-polyethylene glycol-folic acid in a mass ratio of 1:1.7:1.7 The organic solvent was removed by rotary evaporation in an organic solvent to obtain a film material. 2.5mL ultrapure water was added, ultrasonicated by ultrasonic sonicator for 5min, and ultrafiltered by ultrafiltration for 3 times, which resulted in the inclusion of phthalocyanine green phospholipid folic acid targeting nano photosensitizer.

The invention provides a laser nano-optical diagnosis and treatment device. As shown in FIG. 1 , the device comprises: a treatment darkroom 101, a carrying platform 102, a first laser 103, a charge-coupled image sensor CCD (Charge-coupled Device) 104, and a filter. 105. Image processor 106, central controller 107, rotating stage 108, and second laser 109.

In a specific implementation, the treatment darkroom 101 may be made of plastic, metal, wood or stone.

The carrying platform 102 is disposed in the treatment dark room 101 for carrying a therapeutic object injected with a nano photosensitizer in vivo.

The first laser 103 is disposed in the treatment dark room 101 for emitting laser irradiation to the treatment subject to illuminate the nano photosensitizer injected into the treatment subject.

The CCD 104 is disposed in the treatment dark room 101.

The filter 105 is disposed between the treatment target and the CCD 104 for filtering out the light of the set spectrum to transmit the light emitted by the nano photosensitizer.

In the present invention, the filter 105 can achieve the purpose of filtering out stray light and improve the accuracy of positioning the tumor according to the light emitted by the nano photosensitizer. In a specific implementation, the filter 105 needs to cooperate with the nano photosensitizer injected into the body of the treatment object to achieve the purpose of transmitting the light emitted by the nano photosensitizer and filtering out the stray light.

The CCD 104 is configured to photograph the treatment subject through the filter 105.

The image processor 106 is connected to the CCD 104 for acquiring an image captured by the CCD 104, and performing image processing on the image to locate an area enriched in the body of the treatment object.

In a specific implementation, the nano photosensitizer can achieve targeted recognition and enrichment of the tumor, and thus the region enriched in the luminescence in the image of the treated subject is the tumor region.

The rotary table 108 is disposed in the treatment dark room 101; the second laser 109 is mounted on the rotary table 108; and the rotary table 108 adjusts the emission direction of the second laser 109 by rotation.

The central controller 107 is connected to the image processor 106 and the rotating table 108 to position the region in which the enrichment of illumination in the treatment subject is located as a tumor region, and controls the rotation of the rotary table 108 to adjust the emission direction of the second laser 109 to the tumor region. .

a second laser 109 for emitting laser light to irradiate the tumor region, so that the nano photosensitizer exerts photothermotherapy and/or photodynamic therapy;

The central controller 107 is also coupled to the first laser 103 and the second laser 109 for controlling the first laser 103 and the second laser 109 to emit laser light on or off.

Specifically, the central controller 107 can include two control circuits for controlling the activation and deactivation of the first laser 103 and the second laser 109, respectively, and controlling the wavelength and power of the emitted laser light.

Specifically, the wavelength and power of the laser light emitted by the first laser 103 are matched with the nano photosensitizer injected into the body of the treatment object to achieve the purpose of illuminating. Preferably, the laser light emitted by the first laser 103 has a wavelength in the range of 420-800 nm.

Specifically, the wavelength and power of the laser light emitted by the second laser 109 also need to be matched with the nano photosensitizer injected into the body of the treatment object to achieve the purpose of performing photothermotherapy or photodynamic therapy. Preferably, the laser light emitted by the second laser 109 has a wavelength in the range of 600 to 1400 nm.

In a preferred embodiment, the laser nano-optic diagnostic apparatus shown in FIG. 1 may further include an infrared camera, which is composed of an infrared probe, an infrared thermal image processor, and a thermal image display; An infrared probe is disposed in the treatment dark room for receiving infrared rays emitted by the treatment object; and an infrared thermal image processor is connected to the infrared probe for generating a corresponding temperature distribution image according to infrared rays emitted by the treatment object a thermal image display connected to the infrared thermal image processor to display the temperature distribution image. In this way, the relevant medical personnel can pass The thermal image display can observe the local or overall temperature of the treated object in real time, which is beneficial to timely adjust the treatment process to improve the therapeutic effect.

In another preferred embodiment, the laser nano-optical diagnosis and treatment apparatus shown in FIG. 1 may further include an oximeter; the oximeter is composed of a monitoring probe and a data display; wherein the monitoring probe is disposed in the treatment In the dark room, the treatment object is connected for measuring blood oxygen concentration of the tumor area; and a data display is connected to the monitoring probe for displaying blood oxygen concentration data measured by the monitoring probe. In this way, the relevant medical personnel can monitor the changes of blood oxygen concentration in the tumor area through the data display, which is beneficial to timely grasping the therapeutic effect and timely adjusting the treatment process and the treatment time.

In a preferred embodiment, the laser nano-optical diagnostic apparatus shown in FIG. 1 can also provide an illumination source in the treatment darkroom for illuminating the treatment darkroom to facilitate positioning of various instruments in the darkroom. Adjustment, such as aligning the laser to the subject or tumor area.

In a specific implementation, the illumination source may be a circle of LED lights evenly distributed in the treatment darkroom.

Embodiment 1

As shown in FIG. 2, the embodiment is a specific laser nano-optical diagnosis and treatment device, which comprises: a treatment darkroom 201, a carrying platform 202, a first laser 203, a CCD 204, a filter 205, an image processor 206, and a central control. The 207, the rotating 208, the second laser 209, the infrared camera, the oximeter, the illumination source; wherein, the infrared camera comprises: an infrared probe 210, an infrared thermal image processor 211 and a thermal image display 212; The instrument includes: a monitoring probe 213 and a data display 214; the illumination source includes: an LED lamp 215.

The central controller 207 includes: a first laser control circuit, a second laser control circuit, a rotary table drive circuit, a lighting control circuit, and a CCD control circuit.

The first laser control circuit connects the first laser 203 through the optical fiber, and controls the first laser 203 to emit laser light that sets the wave field and power to cause the nano photosensitizer injected into the body of the treatment object to emit light.

The second laser control circuit connects the second laser 209 through the optical fiber, and controls the second laser 209 to emit laser light that sets the wave field and power, so that the nano photosensitizer injected into the therapeutic body functions as a photothermal therapy and/or a photodynamic therapy. .

The rotary stage drive circuit is coupled to the rotary stage 208 to control the rotation of the rotary stage 208 to adjust the emission direction of the second laser 209.

The lighting control circuit connects the illumination source through wires to control the opening and closing of the illumination source.

The CCD control circuit connects the CCD 204 through the data line, controls the activation and deactivation of the CCD 204, and transmits the image information captured by the CCD 204 to the image processor 206.

The rotary stage drive circuit controls the rotation of the rotary stage 208 in accordance with the processing result of the image processor 206 to cause the second laser 209 to emit laser light in alignment with the tumor area.

Embodiment 2

In this embodiment, the laser nano-optical diagnosis and treatment device shown in FIG. 2 is used to perform photothermal treatment on the mouse.

The specific operation procedure was as follows: after the mice were anesthetized, 200 μg/mL of the encapsulated phthalocyanine green phospholipid folate was used to target the nano-photosensitive agent 200 μL in the tail vein injection, and the anesthetized mice were placed on the carrying platform. The first laser emits a laser of 704 nm, illuminates the mouse, and the corresponding wavelength of the filter is 735 nm. The CCD photographs the mouse; the nano-photosensitive agent determines the tumor area after the tumor reaches maximum enrichment; and the second laser emits 808 nm. The laser was adjusted to a power of 1 W/m ² and irradiated in the tumor area; the temperature changes of the mouse and the tumor were observed by an infrared camera.

Embodiment 3

In this embodiment, a laser nano-optical diagnosis and treatment device as shown in FIG. 2 is used to perform photodynamic therapy on mice.

The specific operation procedure was as follows: after the mice were anesthetized, 200 μg/mL of the encapsulated phthalocyanine green phospholipid folate was used to target the nano-photosensitive agent 200 μL in the tail vein injection, and the anesthetized mice were placed on the carrying platform. The first laser emits a laser of 704 nm, illuminates the mouse, and the corresponding wavelength of the filter is 735 nm. The CCD photographs the mouse; the nano-photosensitive agent determines the tumor area after the tumor reaches maximum enrichment; and the second laser emits 670 nm. The laser was adjusted to a power of 50 mW/m ² and irradiated in the tumor area; the blood oxygen concentration of the tumor position of the mouse was observed by an oximeter.

The laser nano-optical diagnosis and treatment device provided by the invention emits laser to inject nano-photosensitive agent injected into the treatment object, and monitors the metabolism, distribution and enrichment of the nano photosensitizer in real time, thereby accurately positioning the tumor position through the tumor region. The nano-photosensitive agent emits laser light to enable it to perform photothermal therapy or photodynamic therapy, and achieve non-invasive and non-invasive tumor treatment for the treated subject.

The above described specific embodiments of the present invention are further described in detail, and are intended to be illustrative of the embodiments of the present invention. All modifications, equivalent substitutions, improvements, etc., made within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims (6)

1. A laser nano-optical diagnosis and treatment device, comprising:

   Treating darkrooms;

   a carrying platform disposed in the treatment dark room for carrying a therapeutic object injected with a nano photosensitizer in vivo;

   a first laser disposed in the treatment dark chamber for emitting laser light to illuminate the treatment object, causing the nano photosensitizer injected into the body of the treatment object to emit light;

   a charge coupled image sensor CCD disposed in the treatment darkroom;

   a filter disposed between the treatment target and the CCD for filtering light of a set spectrum to transmit light emitted by the nano photosensitizer;

   The CCD is configured to photograph the treatment object through the filter;

   An image processor, connected to the CCD, for acquiring an image captured by the CCD, and performing image processing on the image to locate an area enriched in the body of the treatment object;

   a rotary table disposed in the treatment darkroom;

   a second laser mounted on the rotating table;

   The rotating table adjusts a transmitting direction of the second laser by rotating;

   a central controller that connects the image processor and the rotating table, positions an area in which the illumination object is enriched in the treatment object as a tumor area, and controls rotation of the rotating table to adjust an emission direction alignment of the second laser Tumor area;

   The second laser is configured to emit laser light to illuminate the tumor region, so that the nano photosensitizer exerts photothermotherapy and/or photodynamic therapy;

   The central controller is further connected to the first laser and the second laser for controlling the first laser and the second laser to be turned on or off.
2. The laser nano-optical diagnosis and treatment apparatus according to claim 1, further comprising: an infrared camera;

   The infrared camera includes: an infrared probe, an infrared thermal image processor, and a thermal image display;

   The infrared probe is disposed in the treatment dark room for receiving infrared rays emitted by the treatment object;

   An infrared thermal image processor, connected to the infrared probe, for generating a corresponding temperature distribution image according to infrared rays emitted by the treatment object;

   A thermal image display is coupled to the infrared thermal image processor to display the temperature distribution image.
3. The laser nano-optical diagnosis and treatment apparatus according to claim 1, further comprising: an oximeter;

   The oximeter includes: a monitoring probe and a data display;

   The monitoring probe is disposed in the treatment dark chamber and connected to the treatment object for measuring blood oxygen concentration of the tumor region;

   The data display is connected to the monitoring probe for displaying blood oxygen concentration data measured by the monitoring probe.
4. The laser nano-optical diagnosis and treatment device according to claim 1, further comprising:

   An illumination source disposed in the treatment darkroom for illuminating the treatment darkroom.
5. The laser nano-optical diagnosis and treatment apparatus according to claim 1, wherein

   The first laser emits a laser having a wavelength in the range of 420-800 nm.
6. The laser nano-optical diagnosis and treatment apparatus according to claim 1, wherein

   The laser light emitted by the second laser has a wavelength in the range of 600 to 1400 nm.

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