WO2015042950A1

WO2015042950A1 - Bubble-guiding assembly, device for culturing high-density microorganisms and use thereof

Info

Publication number: WO2015042950A1
Application number: PCT/CN2013/084707
Authority: WO
Inventors: 钟琦
Original assignee: 钟琦
Priority date: 2013-09-30
Filing date: 2013-09-30
Publication date: 2015-04-02

Abstract

Disclosed in the present invention is a bubble-guiding assembly, comprising at least two zigzag guide members arranged in the horizontal direction, wherein each zigzag guide member comprises at least two guide plates sequentially connected at the top and bottom in the vertical direction; each guide plate is disposed obliquely relative to the horizontal plane; the oblique directions of any adjacent guide plates relative to the horizontal plane in one and the same zigzag guide member are opposite; a zigzag rising channel is formed between any adjacent zigzag guide members; and any one of the guide plates in a zigzag guide member overlaps with the projecting part of the closest guide plate of an adjacent zigzag guide member in the horizontal plane. The bubble-guiding assembly of the present invention greatly increases the bubble-guided area per unit volume and achieves a modular assembly with a strong adaptability. Also disclosed in the present invention are a device for culturing high-density microorganisms which is able to achieve large-scale production and the use thereof in exhaust gas treatment and microalgae cultures.

Description

Bubble guiding assembly, high-density microbial culture device and application thereof

The present invention relates to the field of microbial culture techniques, and more particularly to a bubble guiding assembly, a high density microbial culture device, and the use of the device in microbial culture and waste gas treatment.

Background technique

The main causes of modern air pollution are industrial exhaust gases from industrial operations of coal-fired oil-fired gas and excessive emissions of exhaust gases from a variety of oil-burning vehicles. The main components include harmful carbon dioxide, nitrogen oxides, sulfur oxides and Carbon monoxide and harmless nitrogen and water vapor. Excessive carbon dioxide in the atmosphere can cause a greenhouse effect and can make the seawater sour, which is the main cause of the deterioration of the world environment. Nitrogen oxides and sulphur oxides do not cause greenhouse effects, but these gases can turn into acids when they are in contact with water. Acid rain is the formation of acid when these gases become water. Acid rain drops to the surface, which can lead to the loss of organic matter in the soil, depleting the soil, and causing the acidification of surface water sources to reduce or even eliminate aquatic organisms and destroy the ecological environment. Acid rain is suspended in the air or is a cause of cockroaches that can cause serious air pollution. There is currently no mature exhaust gas treatment technology that can control carbon dioxide emissions. There is still no unified understanding of the direction of development of its processing technology. Submarine burial and algae carbon sequestration are two representative research hotspots. The technology of seabed burial is high and the investment is large. In contrast, algae carbon sequestration has a broader base. Many countries are experimenting with algae carbon sequestration. The methods are different and the results vary, but there is still no breakthrough.

The microbial environmental protection system replaces the chemical reaction with the growth of microorganisms, and allows the microorganisms to effectively use the artificial environment provided for growth. The sulfur oxides, nitrogen oxides and carbon dioxide in the exhaust gas are naturally soluble in water for the growth of microorganisms, and the microbial environmental protection system is used for both gas absorption and microbial culture. The center of the microbial environmental protection system is biological carbon sequestration. Most of the microorganisms selected are various microalgae. The cultivation methods can be divided into open and closed. Open cultivation mainly uses tracks and ponds, which has the advantages of low construction and use costs and ease of work. It is industrialized and easy to clean; its shortcomings are low production efficiency, easy to be polluted, large land occupation and high water consumption. Closed systems are mainly various photoreactors, which have the advantages of high production efficiency, low pollution, small footprint and low water consumption. The disadvantages are high construction and use costs, difficulty in cleaning, and large-scale industrial application. As one of the research and development priorities, the industry has been looking for a culture device that can be used both for industrial production and high efficiency, but there has been no new breakthrough for many years. The existing microalgae culture system can not meet the needs of carbon fixation, and can not achieve the purpose of industrial production of microalgae.

In the process of microbial culture, it is usually necessary to transfer gaseous nutrients as bubbles to the culture medium of the microorganisms, especially photosynthetic microorganisms such as algae, in the presence of solar radiation and carbon-rich gases such as carbon dioxide. Growth and photosynthesis are carried out in a suitable nutrient culture medium, thereby increasing the supply of gaseous nutrients in the microbial culture solution to facilitate the growth of microorganisms. At present, there are three ways to increase the supply of gaseous nutrients: The first is to increase the gas transmission rate, and press more gas into the microbial culture environment for a certain period of time; the second is to reduce the diameter of the bubbles so that the bubbles can be dissolved. In the culture solution; the third is to increase the height of the microbial culture solution experienced by the bubbles in the incubator. The disadvantages of these three methods are: 1 will increase the energy consumption of the microbial culture process, thereby increasing the production cost; 2 can not enlarge the mode of the microbial incubator to the scale of industrial production; 3 can not use the microbial culture with only a small amount of microbial culture solution The culture is limited, and the application of the microbial incubator is limited. 4 For microalgae cultivation, the prior art can only provide carbon dioxide gas and light energy to a small part of microalgae cells for photosynthesis, thereby reducing the culture efficiency.

Summary of the invention

SUMMARY OF THE INVENTION The object of the present invention is to provide a bubble guiding assembly and a high-density micro-culture device and application thereof which are low in energy consumption, high in efficiency, and modularly assembled, in view of the problems and deficiencies of the prior art.

The technical solution of the present invention is achieved as follows: a bubble guiding assembly comprising at least two meandering guiding members arranged in a horizontal direction; the meandering guiding member comprising at least two guiding plates which are sequentially connected up and down in a vertical direction, The guide plate is inclined relative to the horizontal plane And any adjacent guide plates of the same meandering guide member are opposite to the horizontal plane, and any adjacent curved guide members form a meandering passage; any guide plate of the meandering guide member and adjacent meandering The closest guide plates of the guide members overlap in the projected portion of the horizontal plane.

The bubble guiding assembly further includes a fixing member for fixing the meandering guiding member. The bubble guiding assembly of the present invention greatly increases the area of bubble guiding per unit volume, and realizes modularization, can be easily assembled as needed, and can be applied to large-scale cultivation of microorganisms.

As a further improvement, the guide plate is a flat plate or a curved plate or a wavy plate. When the guide plate is a flat plate, its angle with the horizontal plane is preferably 10-30.

As a further improvement, the interval between the adjacent meandering guide members is 1 5 - 5 0 mm. The present invention also provides a high-density microorganism culture apparatus comprising a container, a bubble generating device, at least one of the bubble guiding assemblies, and the bubble generating device and the bubble guiding assembly are disposed in the container The bubble generating device is disposed at the bottom of the bubble guiding assembly.

Further, a gap is left between the bubble guiding assembly and the inner wall of the container for the flow of the culture solution. A light source, such as an LED lamp, may also be disposed on the meandering guide member or on the inner wall of the container.

The present invention also provides the use of the high-density microbial culture apparatus in exhaust gas treatment, the method of exhaust gas treatment comprising: adding a culture liquid in a container of a high-density microbial culture apparatus and introducing a microorganism including carbon dioxide as a carbon source, a microorganism capable of metabolizing sulfur oxides, a microorganism capable of metabolizing nitrogen oxides, and a microorganism to be cultured, and introducing the exhaust gas into the bubble generating device to form bubbles in the culture liquid, the bubbles from the bubble guiding assembly The bottom enters the channel and climbs along the lower surface of the deflector to provide gas nutrition to the microorganisms.

The invention also provides the application of the high-density microbial culture device in microalgae cultivation, comprising adding a culture liquid in a container of a high-density microbial culture device and introducing the microalgae into the container. Line culture, and introducing a carbon dioxide-containing gas into the bubble generating device to form bubbles in the culture liquid, the bubbles entering the channel from the bottom of the bubble guiding assembly and climbing along the lower surface of the guiding plate to supply gas to microorganisms Nutrition, the light source set during the cultivation provides light energy to the microalgae.

The beneficial effects of the invention are:

The special structure of the bubble guiding assembly provided by the invention greatly improves the area of the bubble guiding and realizes modular assembly. In the high-density microbial culture device of the present invention, the guide plate in the bubble guiding assembly provides a tortuous upward traveling path for the bubble carrying the nutrient gas required for the microbial culture process, so that the bubble rises vertically during the process of ascending along the lower surface of the guide plate. The slower speed increases the horizontal movement, multiplies the contact area between the bubbles and the microbial culture medium per unit volume, and prolongs the gas-liquid contact time, so that the nutrient gas required for microbial growth can be sufficiently transmitted from the gas phase to Microbial culture solution is used for microbial growth, which greatly improves the material and energy utilization efficiency in the microbial culture process, thereby improving the culture efficiency and reducing the cultivation cost. At the same time, the tortuous upward movement process of the bubble increases the gas-liquid agitation frequency, which drives The movement of the microbial culture solution uniformly transfers the nutrients required for the growth of the microorganisms to the entire microbial culture solution, which is advantageous for improving the cultivation efficiency of the microorganisms, and the design and assembly method of the bubble guiding assembly is flexible, and the modularization and the adaptability are strong. . The high-density microbial culture device is applied to waste gas treatment and microalgae cultivation, and the production capacity can reach the level of industrial production.

DRAWINGS

Figure 1 is a schematic view showing the structure of a plurality of bubble guiding members when the guide plates of the meandering guide members are a plurality of;

Figure 2 is an exploded perspective view of the assembly of the bubble guiding assembly when the guiding plate is a square plate;

Figure 3 is a schematic structural view of the assembly of the bubble guiding assembly when the guiding plate is a square plate;

Figure 4 is a view of the bubble guiding assembly when the guide plate of the meandering guide member is two flat plates Schematic diagram

Figure 5 is a schematic view showing the structure of the bubble guiding member when the guide plates of the meandering guide member are two flat plates;

Figure 6 is a schematic structural view of the assembly of the bubble guiding assembly when the guiding plate is a curved plate;

Figure 8 is a schematic view showing the structure of the bubble guiding assembly adopting another assembling manner; Figure 9 is a partial enlarged view A of Figure 8;

Figure 10 is a schematic structural view of a meandering guide member in the bubble guiding assembly of Figure 8; Figure 11 is a schematic cross-sectional view of a high-density microbial culture device according to an embodiment of the present invention; Figure 12 is a structural decomposition of a high-density microbial culture device according to an embodiment of the present invention; BRIEF DESCRIPTION OF THE DRAWINGS Fig. 13 is a perspective view of a high-density microorganism culture apparatus according to an embodiment of the present invention.

detailed description

The invention is further described below in conjunction with the drawings and specific embodiments.

Figures 1 - 5 show a preferred embodiment of the mass production of the convenient guide plates and the assembly of the bubble guide assembly. As shown in FIG. 1 , which is a schematic structural view of a bubble guiding assembly, the bubble guiding assembly 1 includes a plurality of meandering guiding members 10 arranged at equal intervals in a horizontal direction, and the meandering guiding members 10 include a plurality of guiding guides which are sequentially connected up and down in the vertical direction. The plate 101 and the guide plate 101 are inclined with respect to the horizontal plane. The inclination direction of any adjacent guide plates 101 in the same meandering guide member 10 is opposite to the horizontal plane, and any adjacent zigzag guide members 10 form a meandering passage between the adjacent zigzag guide members 10, and the meandering Any of the guide plates 101 of the guide member 10 overlaps with the projected portion of the nearest guide plate 101 of the adjacent meander guide member 10 at the horizontal plane, and the air bubbles enter the passage from the bottom of the bubble guide assembly and climb along the lower surface of the guide plate 101.

In FIG. 1, the assembly method of the bubble guiding assembly 1 is to fix a plurality of guiding plates 101 arranged in parallel in parallel in the horizontal direction to obtain an assembly, and then superimpose the bubble guiding assembly 1 in the vertical direction, specifically flipping the assembly 180. ° , get another assembly, will The assembly before and after the flipping is superimposed, so that the guide plates 101 are in contact with each other and are inclined opposite to the horizontal plane, and the bubble guiding assembly 1 is obtained, which can be further fixed after being superposed to prevent the guiding plate from moving.

As shown in Fig. 2, when the guide plate is a square flat plate, an exploded view of the assembly of the bubble guiding assembly. The fitting includes a guide plate 101 and a fixing member, and the fixing member includes a horizontal strip 111 and a wedge-shaped socket 112 having an oblique slot 1110 at an acute angle to the horizontal direction, and further includes a fastener 113 having a wedge-shaped socket 112 thereon The groove 111 corresponds to the groove 1120. The thickness of the wedge-shaped cap 112 is the same as the vertical height of the guide plate 101, and the surface adjacent to the guide plate 101 is parallel to the guide plate 101.

3 is a schematic structural view of the assembly of the bubble guiding assembly when the guiding plate is a square flat plate, and the guiding plate 101 is fixed by the horizontal strip 111 at the front and the rear of the guiding plate 101 which are equally spaced in a plurality of horizontal directions, at the end The wedge-shaped platform 112 is disposed adjacent to the guide plates 101 at both ends, so that a channel is formed between the inclined surface of the wedge-shaped platform 112 and the guide plate 101 at the end, and the two ends of the horizontal strip 111 are respectively inserted into the 1120 on the wedge-shaped platform 112. The fastener 113 is screwed to obtain an assembly.

Fig. 4 is a schematic view showing the decomposition of the bubble guiding assembly when the guide plates of the meandering guide member are two flat plates, and Fig. 5 is a structural schematic view of the corresponding bubble guiding assembly. The bubble guiding assembly is obtained by superimposing two assembly parts. The exploded view and structure of the assembly are shown in Fig. 2 and Fig. 3, and one assembly is flipped 180. Superimposed on another assembly to obtain the bubble guiding assembly 1 of Fig. 5. The bubble guiding assembly of Fig. 1 can be assembled by a plurality of bubble guiding assemblies of Fig. 5.

In the bubble guiding assembly 1 shown in FIG. 5, the meandering guiding member 10 is composed of two guiding plates 101. In the adjacent zigzag guiding members 10, any adjacent zigzag guiding members 10 form a meandering passage 2, which is meandering. Any of the guide plates 101a of the guide members 10 partially overlaps the projection of the closest guide plates 101b and the guide plates 101c of the adjacent meander guide members 10 in the horizontal plane. The projection of the guide plate 101d of the meandering guide member 10 and the closest guide plate 101e of the adjacent meandering guide member 10 and the projection of the guide plate 101f in the horizontal plane also partially overlap. The bubble 3 is in the channel 2, and climbs to the end along the lower surface of the guide plate 101d. Vertically ascending, reaching the lower surface of the guide plate 101b located slightly above the adjacent meandering guide member 10, and continuing to climb upward along the lower surface, thereby alternately following the guide plates 101 of the adjacent two meandering guide members 10 The surface twists and climbs upwards.

6 is a schematic structural view of the assembly of the bubble guiding assembly when the guiding plate is a curved plate, the assembly includes the guiding plate 101 and the fixing member, and the structure of the fixing member and the assembling manner of the assembly are referred to FIG. 2 and FIG. The guide plate 101 adopts a curved plate to further lengthen the upward path length of the bubble.

Fig. 7 is a schematic view showing the structure of the bubble guiding assembly when the guide plate is a curved plate, and the assembly of Fig. 6 is turned over 180. After being superimposed with the un-turned assembly, the guiding plate 101 is brought into contact to obtain the bubble guiding assembly 1, including the meandering guiding member 10 and the meandering channel 2, and the air bubbles alternately along the guiding plates of the adjacent two zigzag guiding members 10 The lower surface of the 101 zigzag climbs up.

In the bubble guiding assembly 1 of Figs. 1, 5 and 7, the guiding plate 101 on the meandering guiding member 10 is connected by contact, that is, the guiding plate 101 is independently detachable, so that the guiding plates 101 can be arranged in the horizontal direction. The assembled and fixed assembly method is adopted to realize modular assembly, and the assembly method is flexible and convenient. At the same time, the guide plates 101 are arranged at equal intervals in the horizontal direction, and are parallel to each other. While increasing the density of the guide plates 101 and the gas-liquid contact area per unit volume, the spacing between the adjacent guide plates on the adjacent zigzag guide members is smaller. The larger the area of the overlapping portion of the projection on the horizontal surface, the longer the upward path of the bubble on the lower surface of the guide plate, and the larger the gas-liquid contact area.

Fig. 8 is a structural schematic view of a bubble guiding assembly adopting another assembling manner, and Fig. 9 is a partial enlarged view A of Fig. 8. In the bubble guiding assembly 1, the guiding plate 101 is inclined with respect to a horizontal plane, and the plurality of guiding plates 101 are formed in a vertical direction to form a meandering guiding member 10 in an integrated manner, and any adjacent guiding of the same zigzag guiding member 10 The plate 101 is oppositely inclined with respect to the horizontal plane, and a plurality of meandering guide members 10 are disposed at equal intervals in the horizontal direction, and are fixed by the fixing members.

The fixing member includes a card strip 1 14 disposed at both ends of the zigzag guiding member 10 and has a card The strips 114 of the slot 114 of the slot 114 are tightly fitted, and the plurality of meandering guide members 10 are fixed by the rails 115 to obtain the bubble guiding assembly 1.

Figure 10 is a schematic view showing the structure of the meandering guide member 10 in the bubble guiding assembly 1 of Figure 8, which shows the positional relationship of the adjacent meandering guiding members 10, that is, the zigzag rise between any adjacent zigzag guiding members 10 The passage, any one of the guide plates 101 of the meandering guide member 10 overlaps with the projected portion of the nearest guide plate 101 of the adjacent meandering guide member 10 in the horizontal plane, so that the air bubbles alternately follow the guide plates 101 of the adjacent two meandering guide members 10. The surface of the bubble guiding member 10 is climbed upward, and the upper and lower ends of the bubble guiding member 10 are respectively provided with the strips 1 14 for convenient fixing.

The bubble guiding assembly 1 in Fig. 8 adopts a processing method in which the guiding plates 101 are first fixedly connected in series or a plurality of guiding plates 101 are integrally molded at a time, that is, the guiding plates are self-contained, and after the meandering guiding members 10 are obtained, The plurality of meandering guide members 10 are combined and fixedly assembled, which facilitates the production of the meandering guide members 10 and is flexible in assembly. At the same time, the guide plates 101 are equally spaced in the horizontal direction and parallel to each other, which can increase the density of the guide plates 101 per unit volume and the gas-liquid contact area, and the smaller the pitch of the adjacent zigzag guide members, the overlapping projection of the guide plates on the horizontal plane. The larger the partial area, the longer the upward path of the bubble on the lower surface of the guide plate, and the larger the gas-liquid contact area.

In the bubble guiding assembly of FIG. 5 and FIG. 8 , the guiding plate and the meandering guiding member are fixed by the fixing member, and the fixing member and the fixing manner are various, and the guiding plate and the meandering guiding member are fixed, and the traveling path and the cultivation of the bubble zigzag upward are not affected. The flow of liquid can be.

The guide plate in the bubble guiding assembly is a thin plate which cannot be penetrated by bubbles, and is made of plastic, metal or glass or other substances insoluble in the culture liquid. When light is required during the cultivation of microorganisms, it is preferably a light-transmitting plate. At the same time, the bubble is subjected to a vertical upward force or a bubble traveling speed in the culture solution, and when the impulse is large, the bubble can be pushed along the guide plate. Therefore, the specific shape of the guide plate and the inclination angle with respect to the horizontal plane are not strictly limited, and the air bubbles can be lifted up without being blocked, resulting in complete suspension, such as a wave shape, an arc shape or a plane, and a mixture of several shapes. Feasible, using curved surfaces such as curved plates or waves The wave plate can further increase the length of the bubble travel path. When the guide plate is a flat plate, the angle with the horizontal plane is preferably 10-30°, more preferably 20°.

It should be pointed out that when the bubble climbs upward, the guide plate is inclined to the horizontal plane, the bubble movement rate is slow, the gas-liquid agitation is low, but the gas-liquid contact time is long, the path length of the bubble operation or the gas-liquid contact area is large, and the inclination is When the degree is large, the bubble moves at a faster rate, the gas-liquid agitation is large, and the gas-liquid contact time is short, and the total path of the bubble operation or the gas-liquid contact area is small.

The specific operation of the microbial culture may further include pretreatment such as preparation of the culture medium and conventional sterilization of the culture solution, and separation and purification after the completion of the culture.

A specific embodiment of microbial culture is provided below, and the microbial culture method comprises the following steps:

The microorganism to be cultured is the microalgae Synechococcus sp. MAI 9, and the medium BG11 is prepared by adding 1.5 g of sodium nitrate, 0.04 g of dipotassium hydrogen phosphate, and magnesium sulfate (MgS0 ₄ ·7Η ₂ 0 ).

0.075 g, calcium chloride (CaCl ₂ · 2H ₂ 0 ) 0.036 g, citric acid 0.006 g, ammonium ferric citrate 0.006 g, EDTA 0.001 g, sodium carbonate 0.02 g, trace metal solution 1.0 ml, mixed and dissolved in 1 L distilled water Adjust ρΗ to 7.1, sterilize and put in the refrigerator for use. The method for disposing the trace metal solution is: 2.86 g of boric acid, manganese chloride (MnCl ₂ ·4Η ₂ 0 )

1.81 g, sulfuric acid (ZnS0 ₄ ·7Η ₂ 0 ) 0.222 g, sodium molybdate (NaMo0 ₄ ·2Η ₂ 0 ) 0.39g, copper sulfate (CuS0 ₄ · 5H ₂ 0 ) 0.079g, cobalt nitrate (Co (N0 ₃ ₂ · 6H ₂ 0 ) 49.4mg, dissolved in 1.0L of distilled water.

The process of introducing the microalgae into the culture solution of the incubator is first pre-cultured, and the pre-culture includes the following steps:

501. Mix 10 ml of microalgae solution with 30 ml of medium BG11 in an Erlenmeyer flask, and incubate for 24 hours at 25 ° C under LED light irradiation and continuous gas flow to obtain algae culture solution 1 which is slightly turbid green. Liquid

After 502, 3 days, 35ml microalgae culture solution 1 and 165ml medium BG11 mixed, continue to culture, the culture conditions are the same as S01, to obtain microalgae culture solution 2; After 503 and 3 days, the 200 ml microalgae culture solution 3 obtained by S02 was divided into three equal portions and placed in an Erlenmeyer flask. The medium BG11 was added to 200 ml, and the culture was continued. The conditions were the same as those of S01, until the microalgae culture solution was changed. Turbid, obtaining microalgae culture solution 3;

504. After 1 day, the microalgae culture solution 3 in the three conical flasks was combined into a conical flask, and the medium BG11 to 2L was added to continue the culture. The culture condition was the same as that of S01 to obtain a green turbid microalgae preculture solution. .

The pre-cultured microalgae preculture solution is added to the container of the high-density microbial culture device, and 18 L of the medium BG11 is added to open the LED lamp in the high-density microbial culture device, and the gas is continuously supplied and cultured.

The pre-culture and culture process of the microalgae is air containing 15% (by volume) of carbon dioxide, OOppm of nitrogen dioxide, and 250 ppm of sulfur dioxide. The amount of intake air during the cultivation is 1 cubic meter per minute.

The microorganism culture method of the present invention can also be used for cultivating bacteria. When the cultured bacteria is E. col i, the preparation method of the culture medium LB is: tryptone l Og, yeast extract (yeas t extract) 5 g, chlorine Sodium (NaCl) 10g, agar powder 15_20g, dissolved in l OOOmL double-distilled water, using a 5mol / L NaOH solution about 0. 2ml to adjust the pH to 7.2, then sterilized at 121 °C for 30min after use. The bacteria to be cultured E. col i was introduced into the medium LB in the culture vessel, and the gas was introduced and cultured.

11 is a schematic cross-sectional view of a high-density microbial culture device, FIG. 12 is a schematic exploded view of a high-density microbial culture device, and FIG. 13 is a perspective view of a high-density microbial culture device including a container and a container disposed therein. The bubble guiding unit 1, the bubble generating device 6, and a gas introducing device.

The container is specifically an incubator, and includes a box body 41, a box cover 42 and an air outlet hole 43. The bottom surface of the box body 41 is about 1 X lm ² and the height is about lm. The liquid level of the culture liquid in the incubator is higher than the bubble guide assembly 1 and the box. The body 41 and the cover 42 are respectively made of plastic or metal or glass or other materials, and are not particularly limited.

The gas introduction device includes a gas distributor 51 and an intake duct 52 through which the gas passes through the intake duct 52. After being introduced into the air chamber at the bottom of the container, bubbles having a smaller diameter are generated in the culture liquid through the bubble generating device 6, and are entered from the bottom of the bubble guiding unit 1, and the bubbles 3 are overflowed after being exchanged with the culture liquid containing the microorganism to be cultured. The liquid is discharged from the air outlet 43. The bubble generating device 6 is a microplate, and a gasket 7 for sealing is provided between the gas chamber and the bubble generating device. The bubble generating device may also be a microporous tube or a microporous aerator or other device that can form a gas bubble in the culture solution.

Refer to Figures 1 - 5 for the structure and assembly of the bubble guide assembly 1. The zigzag guide member and the fixing member are arranged, and the guide plates of the same size are arranged and fixed at equal intervals in the horizontal direction to obtain an assembly, which will be flipped 180. The rear fitting is superimposed with the unrotated fitting to connect the guide plates on the meandering guide member to each other to obtain a bubble guiding assembly composed of a plurality of meandering guiding members. Specifically, the guide plate is a square transparent plate with a length of 1 m, a width of 10.6 cm and a thickness of l mm, and the angle with the horizontal plane is 20 degrees, and 28 (only a part of the figure is shown) are sequentially contacted vertically in the vertical direction. The connected guide plates constitute a meandering guide member, and 45 (two parts are shown in the figure) are arranged at equal intervals (20 匪) in the horizontal direction. The zigzag guide members are fixed to obtain a bubble guide assembly, and the bubbles alternate along two adjacent turns. The lower surface of the guide plate of the guide member is bent up and up until the culture liquid containing the microorganism to be cultured escapes.

After the bubble guiding assembly 1 is disposed in the casing 41, a gap 8 is left between the bubble guiding assembly 1 and the inner wall of the casing 41, and the culture liquid containing the microorganism to be cultured by the bubble is flowed to promote the substance in the culture system. The equilibrium distribution increases the culture efficiency. Specifically, during the cultivation process, the bubbles travel along the meandering path in the channel of the bubble guiding assembly 1, and the culture liquid containing the microorganism to be cultured also moves upward, and then proceeds with the bubble. The bubble guiding assembly 1 is returned to the bottom of the casing 41 along the gap 8 between the casing 41 and the bubble guiding assembly 1.

In the incubator, an LED lamp is mounted on the guide plate of the bubble guiding assembly 1 to project light around the bubble, so that the microorganism to be cultured can simultaneously obtain carbon dioxide and light energy necessary for photosynthesis, so that the microorganism to be cultured can effectively perform photosynthesis. Function and rapid growth.

In the bubble guiding assembly 1 of the present embodiment, the bubbles are cultured with microorganisms to be cultured. The length of the travel path in the liquid is 2.99m, the relative vertical travel distance (lm) is increased by nearly 200%, and the contact area of the bubble 3 with the microalgae-containing culture solution is 1 31⁄2 ² , which is more than when it is directly in the vertical direction. The area lm ^{2 is} increased by 34 times, which significantly increases the gas-liquid contact area per unit volume, and promotes gas-liquid material exchange and microbial culture. At the same time, the bubble drives the culture liquid containing the microalgae in the process of the zigzag upward movement, increases the gas-liquid agitation frequency, promotes the material balance, and improves the microbial culture efficiency. As the microorganisms to be cultured continue to divide and grow, the light transmittance of the culture liquid is drastically reduced, and the microorganisms are mainly grown only in a region where light can be received, and the superiority of such a device is fully manifested.

In the microalgae culture process, the growth of the microalgae is measured by observing the green change and detecting the change of the optical density of the culture liquid. The test results show that the device can be used to culture the microalgae, and can process 297 g of carbon dioxide per minute, 0. 26 g. Nitrogen dioxide, 0.66 grams of sulfur dioxide, and 162 grams of microalgal biomass, significantly improved culture efficiency.

When applying such a high-density microbial culture device to waste gas treatment, it is necessary to introduce a corresponding microorganism into the culture liquid, such as introducing carbon dioxide as a carbon source for treating carbon dioxide, introducing a microorganism capable of metabolizing sulfur oxide, and treating it by treating sulfur oxide. Nitric oxide is introduced into a microorganism capable of metabolizing nitrogen oxides, and a bubble generating device that introduces exhaust gas into the culture device during processing forms bubbles in the culture solution to provide gas nutrition to the microorganisms. It is to be understood that the specific implementation of the invention is not limited to the description. It will be apparent to those skilled in the art that the present invention can be made without departing from the spirit and scope of the invention.

Claims

claims

1. A bubble guide assembly, characterized in that: it includes at least two meandering guide members arranged in the horizontal direction; the meandering guide member includes at least two guide plates connected up and down in the vertical direction, the guide plates They are arranged inclined relative to the horizontal plane, and any adjacent guide plates in the same meandering guide member have opposite inclination directions relative to the horizontal plane, and a meandering ascending channel is formed between any adjacent meandering guide members; any guide plate of the meandering guide member is connected to the horizontal plane. Projections of the closest guide plates of adjacent meandering guide members on the horizontal plane partially overlap.

2. The bubble guide assembly according to claim 1, characterized in that: it further includes a fixing member for fixing the meandering guide member.

3. The bubble guide assembly according to claim 2, characterized in that: the guide plate is a flat plate, an arc plate, or a wavy plate.

4. The bubble guide assembly according to claim 3, characterized in that: the guide plate is a flat plate, and its angle with the horizontal plane is 10-30°.

5. The bubble guide assembly according to any one of claims 1 to 4, characterized in that: the interval between adjacent meandering guide members is 15-50mm.

6. A high-density microbial culture device, characterized in that: the high-density microbial culture device includes a container, a bubble generating device, and at least one bubble guide assembly according to any one of claims 1 to 5, the bubble generating device The device and the bubble guide assembly are arranged in the container, and the bubble generating device is arranged at the bottom of the bubble guide assembly.

7. The high-density microbial culture device according to claim 6, characterized in that: there is a gap for the flow of culture liquid between the bubble guide component and the inner wall of the container.

8. The high-density microbial culture device according to claims 6 to 7, characterized in that: the meandering guide member is provided with a light source.

9. Application of the high-density microbial culture device according to any one of claims 6 to 8 in waste gas treatment. The waste gas treatment method includes: adding culture liquid to the container of the high-density microbial culture device and introducing carbon dioxide. Microorganisms as carbon sources can replace Cultivate at least one of microorganisms that metabolize sulfur oxides and microorganisms that can metabolize nitrogen oxides, and introduce the waste gas into the bubble generating device to form bubbles in the culture solution, and the bubbles come from the bottom of the bubble guide assembly Enter the channel and climb along the lower surface of the guide plate to provide gaseous nutrients to the microorganisms.

10. The application of the high-density microbial culture device of claim 8 in microalgae culture, including adding culture liquid into the container of the high-density microbial culture device and introducing microalgae for culture, and introducing carbon dioxide-containing gas into the said The bubble generating device forms bubbles in the culture solution. The bubbles enter the channel from the bottom of the bubble guide component and climb along the lower surface of the guide plate to provide gas nutrients for microorganisms. During the cultivation process, the light source provides microalgae. light energy.