WO2014158290A1 - Optical beam positioning unit for atomic force microscope - Google Patents

Abstract

This invention relates to an optical light beam positioning system that enables the combination of two or more light beams of different wavelengths to be focused onto a probe or sample of a scientific instrument, such as an atomic force microscope, for a number of specific uses typical to AFMs, like measuring the deflection or oscillation of the probe and illuminating an object for optical imaging, and less traditional ones like photothermal excitation of the probe, photothermal activated changes in the sample, photothermal cleaning of the probe and photochemical, photovoltaic, photothermal and other light beam induced changes in the sample. The focused light beams may be independently positioned relative to each other.

Description

OPTICAL BEAM POSITIONING UNIT FOR ATOMIC FORCE MICROSCOPE

REFERENCES CITED

US PATENTS

4724318

5, 047, 633

5, 412, 980

5512745

5144833

4747698

7497613

5441343

6696370

8443459

5003815

5825020

6330824

7497613

8370960

8443459 8458810

US PATENT APPLICATIONS

20130074920 20120096602 20110251059

OTHER PUBLICATIONS

Binnig G, Quate CF, Gerber C. Atomic force microscope. Phys. Rev. Lett. 1986; 56:930.

R. Garcia, Amplitude Modulation Atomic Force Microscopy (Wiley-VCH, einheim, 2010) .

P. Gleyzes, P. K. Kuo, and A. C. Boccara, "BISTABLE BEHAVIOR OF A VIBRATING TIP NEAR A SOLID-SURFACE," Applied Physics Letters 58 (25), 2989-2991 (1991)

Q. Zhong, D. Inniss, K. Kjoller, and V. B. Elings, Surf. Sci. Lett. 290, L688 (1993). D.A. Chernoff, edited by G.W. Bailey et al. (Jones and Begell, New York, 1995), pp. 888-889.

P. Achalla, J. McCormick, T. Hodge et al.,

"Characterization of elastomeric blends by atomic force microscopy," Journal of Polymer Science: Part B 44, 492- 503 (2005);

J.B. Pethica and W.C. Oliver, "Tip surface interactions in STM and AFM, " Physica Scripta T19A, 61-66 (1987).

R. Garcia, J. Tamayo, and A. San Paulo, "Phase contrast and surface energy hysteresis in tapping mode scanning force microscopy," Surface and Interface Analysis 27 (5- 6) , 312-316 (1999) .

Yagun Zhao, Qian Cheng, Menglu Qian et al . , "Phase image contrast mechanism in intermittent contact atomic force microscopy," Journal of Applied Physics 108, 094311 (2010)

Wensheng Xu, Paula M. Wood-Adams, and Christopher G.

Robertson, "Measuring local viscoelastic properties complex materials with tapping mode atomic force

microscopy," Polymer 47, 4798 (2006).

F. Dubourg, JP Aime, S Maursaudon et al., "Probing

viscosity of a polymer melt at the nanometer scale with an oscillating nanotip, " Eur Phys J E6, 49-55 (2001).

G.J.C. Braithwaite and P.F. Luckham, "The simultaneous determination of the forces and viscoelastic properties adsorbed polymer layerse, " Journal of Colloid and

Interface Sciece 218, 917 (1999) .

A. Noy, C.H. Sanders, D.V. Vezenov et al., "Chemically sensitive imaging in tapping mode by chemical force microscopy: Relationship bewteen phase lag and adhesion," Langmuir 14 (7), 1508-1511 (1998).

R. Garcia N.F. Martinez, "Measuring phase shifts and energy dissipation with amplitude modulation AFM, "

Nanotechnology 17, S167-172 (2006).

J. P. Cleveland, B. Anczykowski, A.E. Schmid et al.,

"Energy dissipation in tapping mode atomic force

microscopy," Applied Physics Letters 72, 2613-2615 (1998).

Carlos J. Gomez and Ricardo Garcia, "Determination and simulation of nanoscale energy dissipation processes in ampltiude modulation AFM," Ultramicroscopy, 626-633

(2010) .

A. San Paulo and R. Garcia, "Unifying theory of tapping- mode atomic-force microscopy, " Physical Review B

[Condensed Matter and Materials Physics] 66 (4),

041406/041401-041404 (2002).

Noncontact Atomic Force Microscopy, edited by S. Morita, R. iesendanger, and E. Meyer, Springer, Berlin, (2002).

Giessibl, Rev. Mod. Phys . 75, 949 (2003) R. Garcia and R. Perez, Surf. Sci. Rep. 47, 197 (2002).

Y. Sugimoto, M. Abe, S. Hirayama, N. Oyabu, 0. Custance, and S. Morita, Nat. Mater. 4, 156 (2005).

M. A. Lantz, H. J. Hug, R. Hoffman, P. J. A. van Schendel, P. Kappenberger, S. Martin, A. Baratoff, and H. J.

Guntherodt, Science 291,2580 (2001).

N. Oyabu, P. Pou, Y. Sugimoto, P. Jelinek, M. Abe, S.

Morita, R. Perez, and 0. Custance, Phys . Rev. Lett. 96, 106101 (2006) .

Y. Sugimoto, P. Pou, 0. Custance, P. Jelinek, S. Morita, R. Perez, and M. Abe, Phys. Rev. B 73, 205329 (2005).

Y. Sugimoto, P. Jelinek, P. Pou, M. Abe, S. Morita, R.

Perez, and 0. Custance, Phys. Rev. Lett. 98, 106104 (2007) .

Y. Sugimoto, P. Pou, M. Abe, P. Jelinek, R. Perez, S.

Morita, and 0. Custance, Nature (London) 446, 64 (2007).

J. I. Kilpatrick, A. Gannepalli, J. P. Cleveland and S. P. Jarvis, Rev. Sci. Inst., 80, 023701 (2009).

A. Gannepalli, A. Sebastian, J. Cleveland, and M.

Salapaka, Appl . Phys. Lett. 87, 111901 (2005).

R. Proksch and E.D. Dahlberg, Rev. Sci. Instr. 64, 912 (1993) . M. Stark, R. Guckenberger, A. Stemmer, and R.W. Stark, J. Appl. Phys. 98, 114904 (2005).

R. Proksch, Appl. Phys. Lett. 89, 113121 (2006).

B.J. Rodriguez, C. Callahan, S.V. Kalinin, and R. Proksch, Nanotechnology 18, 475504 (2007) .

M. Baumann and R.W. Stark, Ultramicroscopy 110, 578-581 (2010) .

G. Chawla and S. D Solares, Meas. Sci. Technol . 20, 015501

M. Stark, R.W. Stark, W.M. Heckl, and R. Guckenberger, Proc. Natl. Acad. Sci. USA 99, 8473-8478 (2002).

T.R. Rodriguez and R. Garcia, Appl. Phys. Lett. 84, 449 (2004) .

J.W. Li, J. P. Cleveland, and R. Proksch, Appl. Phys. Lett. 94, 163118 (2009) .

J.R. Lozano and R. Garcia, Phys. Rev. Lett. 100, 076102 (2008) .

Stark, Appl. Phys. Lett. 94, 063109 (2009) D. Platz, E . A. Tholen, D. Pesen, and D. B. Haviland, Appl. Phys. Lett. 92, 153106 (2008).

A.B. Kos and D.C. Hurley, Meas. Sci. Technol . 19, 015504 (2008)

R. Nath, Y.H. Chu, N.A. Polomoff, R. Ramesh, and B.D.

Huey, Appl. Phys. Lett. 93, 072905 (2008).

S. Jesse, S.V. Kalinin, R. Proksch, A. P. Baddorf, and B.J. Rodriguez, Nanotechnology 18, 435503 (2007) .

S. Jesse and S.V. Kalinin, Nanotechnology 20, 085714

(2009) .

S. Jesse, B. Mirman, and S.V. Kalinin, Appl. Phys. Lett. 89, 022906 (2006) .

R. Proksch and D. Yablon, Appl. Phys. Lett., 100, 073106 (2012) .

M. Stark, R. . Stark, . M. Heckl, and R. Guckenberger, Proc. Natl. Acad. Sci. USA 99, 8473 (2002).

T. R. Rodriguez and R. Garcia, Appl. Phys. Lett. 84, 449 (2004) .

R. Proksch, Appl. Phys. Lett. 89, 113 121 (2006) Martinez, S. Patil, J. R. Lozano, and R. Garcia, Phys. Lett. 89, 153 115 (2006).

0. Sahin, S. Magonov, C. Su, C. F. Quate, and 0. Solgaard, Nature Nanotech. 2, 507 (2007).

D. Platz, E. A. Thole ή, D. Pesen, and D. B. Haviland, Appl. Phys. Lett. 92, 153106 (2008).

R. Proksch, D. Yablon, and A. Tsou, ACS Rubber Division 180th Technical Meeting, 2011-24 (2011) .

S. D. Solares and G. Chawla, J. Appl. Phys., 108, 054901 (2010) .

S. Guo, S. D. Solares, V. Mochalin et al., Small 8, 1264 (2012) .

Giessibl, Phys. Rev. B 56(24) 16010 (1997)

D. A. Walters, J. P. Cleveland, N. H. Thomson et al . Rev. Sci. Inst . , 67 3583 (1996) .

K. Yamanaka, H. Ogiso, and 0. Kolosov, Appl. Phys. Lett. 64, 178 (1994) .

U. Rabe and W. Arnold, Appl. Phys. Lett. 64, 1493 (1994). K. Yamanaka et al . , Appl. Phys. Lett. 78, 1939 (2001). U. Rabe et al., J. Phys . D: Appl . Phys . 35 , 2621 (2002)

A. Gannepalli et al . , Nanotechnology 22, 355705 (2011).

M P Nikiforov, S Hohlbauch, P King, K Voitchovsky, S Contera, S Jesse, S V Kalinin and R Proksch,

Nanotechnology, 22 (2011) 055709

J. Lee et al. Journal of Microelectromechanical Systems, 15, 1644-1655, 2006.

Nelson, B. A. and W. P. King, Review of Scientific

Instruments, 78, 023702, 2007.

P Yuya, D Hurley, J Turner J Appl Phys (2008) and (2011) J Killgore et al. Langmuir (2011)

R. Proksch, S. Kalinin, Nanotechnology 21 455705 (2010)

K. Kobayashi, H. Yamada, and K. Matsushige, Review of Scientific Instruments 82, 033702 (2011)

A. Labuda, K. Kobayashi, D. Kiracofe, K. Suzuki, P.

Grutter, and H. Yamada, AIP Advances 1, 022136 (2011)

A. Labuda, K. Kobayashi, K. Suzuki, H. Yamada, and P. Grutter, Phys. Rev. Lett. 110, 066102 (2013)

A. Labuda, K. Kobayashi, Y. Miyahara, P. Grutter, Rev. Sci. Instrum. 83, 053702 (2012)

A. Labuda, P. Grutter, Langmuir 28, 5319 (2012)

BACKGROUND OF THE INVENTION [0001] Scanning probe devices such as the scanning Probe microscope ("SPM") or atomic force microscope ("AFM") can be used to obtain an image or other information indicative of the features .)f a wide range of materials with molecular and even atomic level resolution. In addition, AFMs and SPMs are capable of measuring forces accurately at the piconewton to micronewton range, in a measurement mode known as a force-distance curve or force curve. As the demand for resolution has increased, requiring the measurement of decreasingly smaller forces free of noise artifacts, the old generations of these devices are made obsolete. A demand for faster results, requiring decreasingly smaller cantilevers, only

reinforces this obsolescence. The preferable approach is a new device that addresses the central issue of measuring small forces with minimal noise, while providing an array of modules optimizing the performance of the device when using small cantilevers or when doing specialized

applications such as optical techniques for biology, optical techniques for photochemical, photothermal , photovoltaic and other light induced changes to the cantilever or sample, nanoindentation and

electrochemistry . [0002] For the sake of convenience, the current description focuses on systems and techniques that may be realized in particular embodiments of scanning probe devices, the SPM or the AFM. Scanning probe devices also include such instruments as 3D molecular force probe instruments, scanning tunneling microscopes ("STMs") , high-resolution profilometers (including mechanical stylus profilometers) , surface modification instruments,

nanoindenters , chemical/biological sensing probes,

instruments for electrical measurements and micro-actuated devices. The systems and techniques described herein may be realized in such other scanning probe devices.

[0003] A SPM or AFM is a device which obtains topographical information (and other sample characteristics) while scanning (e.g., rastering) a sharp tip on the end of a probe relative to the surface of the sample. The information and characteristics are obtained by detecting small changes in the deflection or

oscillation of the probe (e.g., by detecting changes in amplitude, deflection, phase, frequency, etc.) and using feedback to return the system to a reference state. By scanning the tip relative to the sample, a map of the sample topography or other characteristics may be

obtained . [0004] Changes in the deflection or oscillation of the probe are typically detected by an optical lever arrangement whereby an incident light beam is directed onto the side of the probe opposite the tip and a reflected beam from the probe illuminates a position sensitive detector ("PSD") . As the deflection or

oscillation of the probe changes, the position of the reflected spot on the PSD also changes, causing a change in the output from the PSD. Changes in the deflection or oscillation of the probe are typically made to trigger a change in the vertical position of the base of the probe relative to the sample (referred to herein as a change in the Z position, where Z is generally orthogonal to the X/Y plane defined by the sample) , in order maintain the deflection or oscillation at a constant pre-set value. It is this feedback that is typically used to generate a SPM or AFM image.

[0005] SPMs or AFMs can be operated in a number of different sample characterization modes, including contact modes where the tip of the probe is in constant contact with the sample surface, and AC modes where the tip makes no contact or only intermittent contact with the surface. [0006] Actuators are commonly used in

SPMs and AFMs, for example to raster the probe or to change the position of the base of the robe relative to the sample surface. The purpose of actuators is to provide relative movement between different parts of the SPM or AFM: for example, between the tip of the probe and the sample. For different purposes and different results, it may be useful to actuate the sample or the tip or some combination of both. Sensors are also commonly used in SPMs and AFMs. They are used to detect movement,

position, or other attributes of various components of the SPM or AFM, including movement created by actuators.

[0007] For the purposes of this

specification, unless otherwise indicated, the term

"actuator" refers to a broad array of devices that convert input signals into physical motion, including piezo activated flexures; piezo tubes; piezo stacks, blocks, bimorphs and unimorphs; linear motors; electrostrictive actuators; electrostatic motors; capaci".:ive motors; voice coil actuators; and magnetostrictive actuators; and the term "sensor" or "position sensor" refers to a device that converts a physical quantity such as displacement, velocity or acceleration into one or more signals such as an electrical signal, including optical deflection detectors (including those referred to above as a PSD and those described in US Patent No. 6,612,160, Apparatus and Method for Isolating and Measuring Movement in Metrology Apparatus); capacitive sensors; inductive sensors

(including eddy current sensors); differential

transformers (such as those described in US Patent No. 7,038,443 and continuations thereof, Linear Variable

Differential Transformers for High Precision Position Measurements; US Patent No. 8,269,485 and continuations thereof, Linear Variable Differential Transformer with Digital Electronics; and US Patent No. 8,502,525, and continuations thereof, Integrated Micro-Actuator and

Linear Variable Differential Transformers for High

Precision Position Measurements, each of which is hereby incorporated by reference in their entirety) ; variable reluctance; optical interferometry; strain gages; piezo sensors; and magnetostrictive and electrostrictive

sensors .

[0008] Some current SPM/AFMs can take images up to 100 urn², but are typically used in the 1 - 10 urn regime. Such images typically require from four to ten minutes to acquire. Efforts are currently being made to move toward what has been called "video rate" imaging. Typically those who use this term include producing image at the rate of one per second all the way to a true video rate at the rate of 30 per second. Video rate imaging would enable imaging moving samples, imaging ephemeral events and simply completing imaging on a timelier basis. One important means for moving toward video rate imaging is to decrease the mass of the probe, thereby achieving a higher resonant frequency while maintaining a lower sprin constant .

BRIEF DESCRIPTION OF THE DRAWINGS

[0009] FIG. 1 : A schematic of an optical beam positioning unit of the present invention optics used to form a focused light beam on the probe or the sample.

[0010] FIG. 2 : Block diagram showing a light path of the present invention with a multiplicity o optical beam positioning units.

[0011] FIG . 3 : Block diagram showing a light path of the present invention with a multiplicity o nested optical beam positioning units. [0012] FIG. 4: Block diagram showing the Steering Mirror of an optical beam positioning unit and the Scheimpflug plane.

[0013] FIG. 5 : Block diagram showing the Steering Mirror of an optical beam positioning unit and the Scheimpflug plane with the physical pivot translated along the x-axis.

[0014] FIG. 6: Block diagram showing the Steering Mirror of an optical beam positioning unit and the Scheimpflug plane with the phys _cal pivot translated along the z-axis.

[0015] FIG. 7 : Block diagram showing the Steering Mirror of an optical beam positioning unit and the Scheimpflug plane with the physical pivot translated along the y-axis.

[0016] FIG. 8: Block diagram showing the Steering Mirror of an optical beam positioning unit and the Scheimpflug plane with the physical pivot translated along the y-axis, z-axis and x-axis.

[0017] FIG. 9: Photographs showing cantilever response to being driven at different frequencies and locations. DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] As already discussed the focused light beam in AFMs is used to measure the deflection or oscillation of the probe. It is desirable however to focus more than one light beam onto the probe to enable

functionalities beyond measuring probe displacement. It is also desirable to focus more than one lj.ght beam onto the sample to enable other functionalities. The present invention resolves the design complications that stem from so focusing multiple light beams onto a single cantilever or the sample by overlapping the multiple light beams along a single optical axis of a single objective lens that is used to focus all the light beams congruently. The angular orientation or direction of travel of each light beam and the axial position of th focus of each light beam relative to the optical axis are controlled independently between light beams to allow for independent control of the three dimensional position of the focus location of each light beam relative to the cantilever or sample .

[0019] FIG. 1 shows a schematic of the optical beam positioning ( OBPU ) unit of the present invention which forms one focused light beam on the probe or the sample. Other focused light beams would employ a similar optical beam positioning unit. In FIG. 1 the light source 100 for the optics emits a divergent beam of light that is substantially collimated oy a lens 104. The light source 100 could be a laser diode or another light source such as a superluminescent diode or light-emitting diode. The only requirement is that the power density be high enough to excite the desired effect in the cantilever or the sample. The lens 104 is preferably aspheric, in order to maximize the quality of the transmitted light beam.

[0020] The collimated (or nearly collimated) light beam exiting the lens 104 may optionally traverse a linear polarizer 108. The linear polarizer 108 can be rotated about the optical axis relative to the light source 100 (or the light source 100 may be rotated relative to the linear polarizer 108) in order to maximize the light power throughput or to tune a desired amount of light throughput if the maximum amount of light power is deemed excessive. Also, tilting the linear polarizer 108 relative to the optical axis may be advantageous as it can reduce the amount of back-reflected light returning into the light source 100. Back-reflected l,.ght may cause instabilities in the light emitting process.

[0021] The polarized light beam is subsequently passed through a lens 112 and refocused.

This lens may be an aspheric lens, achromatic doublet, or other lens or lens group. The light beam then reflects from a steering mirror 116 that is disposed between the lens 112 and the focus of the light beam 124. The

steering mirror 116 is supported so that it can be rotated about a physical pivot 120, defined as a point in three- dimensional space. As will be shown below, rotation of the steering mirror 116 about the physical pivot 120

provides a means for moving a focused spot in two

dimensions in the plane of the probe or the plane of the sample. For the purposes of this specification these two planes are not shown separately in FIG. 1 but are

collectively referred to herein as the target object 178.

[0022] The functioning of the steering mirror 116 is illustrated in detail in FIG. 3. In the preferred embodiment of the present invention, the

steering mirror 116 can be rotated about three orthogonal axes, two of which are parallel to the mirror 116 surface and are important for the purposes of the invention. The y-axis is one of the axes which is parallel to the mirror 116 surface. The y-axis lies within the plane 200 defined by the incident light beam 204 and the reflected light beam 208. The z-axis is the other axis which is parallel to the mirror 116 surface. The z-axis is orthogonal to the plane of incidence 200. Rotating trie steering mirror 116 about the y-axis ("pitching" the steering mirror 116) or about the z-axis ("yawing" the steering mirror 116), or both, changes the direction and focus position of the reflected light beam 204. Rotating the steering mirror 116 about the x-axis ("rolling" the steering mirror 116) however has no effect on the direction of the reflected light beam 208.

[0023] In the preferred embodiment, the steering mirror 116 is provided with means for actuating the pitch and yaw rotations in order to produce the desired changes in the direction and focus of the

reflected light beam 204. This means may preferably be a kinematic stage driven by transducers. The transducers and kinematic stage rotate the steering mirror 116 in two dimensions about the physical pivot 120 These

transducers are preferably fine-pitch leadscrews driven by high-precision stepper motors. Alternately, the means of actuating the pitch and yaw rotations may be a rotary stage, flexure stage, or gimbal stage, and the transducers may be electromechanical motors, DC motors, piezoelectric inertial motors, piezoelectric transducers, or manual positioners. Preferably, if the transducers are stepper motors, they are provided with a gearbox to reduce the mechanical step size such that the positioning of the light beam focus is precise.

[0024] Pitching and/or yawing the steering mirror 116 affects the reflected light beam 208 in two different ways. First, pitching and/or yawing the steering mirror 116 affects the two-dimensional angular orientation or direction of travel of the reflected light beam 208. Second, pitching and/or yawing the steering mirror 116 affects the axial position of the focus of the reflected light beam 208. If the physical pivot 120

(about which the steering mirror 116 can be rotated) is located at the point of incidence 216 (where the incident light beam 204 intersects the reflected light beam 208) , as drawn in Figure 3, the effect of pitching/yawing on the axial position of the focus is minimal. Only the angular orientation of the reflected light beam 208 is affected by pitching and/or yawing under this condition. However, if the physical pivot 120 is translated relative to the point of incidence 216 as shown in FIG. 5, FIG. 6 and FIG. 7, pitching and/or yawing moves the axial position of the focus of the reflected light beam 208 at the same time that it changes the angular orientation of the reflected light beam 208. This is crucial to the present invention. When designing the optical system, the exact location of the physical pivot 120 in three-dimensional space is tuned to set a desired relationship between the axial position of the focus and the angular orientation of the reflected light beam 208. When this relationship is achieved, the axial position of the focus is geometrically constrained to move along a mathematically defined surface, the

"Scheimpflug surface" 124. For small angular changes around the reflected light beam 208 the Scheimpflug surface can be approximated by a "Scheimpflug plane" 212, as drawn in Figure 3. The term Scheimpflug surface 124 refers to an optical principle, the Scheimpflug criterion, which is used to select the desired Scheimpflug plane 124 based on the planes of the target object 178.

[0025] As displayed in Figure 4,

translating the physical pivot 120 relative to the point of incidence 216 along the x-axis has no consequence on the orientation of the Scheimpflug plan>^:i. With the physical pivot 120 translated only along the x-axis, pitching and/or yawing the steering mirror 116 moves the reflected light beam 208 along the same Scheimpflug plane as it would have moved prior to translation of the

physical pivot 120. Therefore, to understand the operation of the present invention, it suffices to discuss the effect of rotating the steering mirror 116 about rotation axes that are in the plane of the mirror surface,

intersecting in a physical pivot 120 that is also in the plane of the mirror surface. It may be noted however that placing the physical pivot 120 along the x-axis a short distance behind the plane of the steering mirror 116 has little effect on performance.

[0026] FIG. 5 illustrates the effect of translating the physical pivot 120 along the z-axis: the Scheimpflug plane 212 is rotated due to simultaneous changes in the axial position of focus and angular

orientation of the reflected light beam 208.

Specifically, the Scheimpflug plane 212 is rotated

("tilted") along an axis that lies parallel to the plane of incidence 200. The position of the focus moves along a tilted focal plane as the steering mirror 116 is yawed or pitched about the physical pivot 120.

[0027] FIG. 6 illustrates the effect of translating the physical pivot 120 along the y-axis: the Scheimpflug plane 212 is rotated ("tipped") along an axis that lies perpendicular to the plane of incidence 200. Now, the position of the focus moves along a tipped focal plane as the steering mirror 116 is yawed or pitched about the physical pivot 120.

[0028] FIG. 7 illustrates that

translations along the z-axis, the y-axis and the x-axis may be combined, with the Scheimpflug plane 212 both

"tilted" along an axis that lies parallel to the plane of incidence 200 and "tipped" along an axis that lies

perpendicular to the plane of incidence 200.

[0029] The Scheimpflug surface 124 has an optical image near the front focal plane 174 of the objective lens 170, which is approximated by the

"conjugate Scheimpflug focal plane" 180 as shown in FIG. 1. The tilt angle of the conjugate Scheimpflug focal plane 180 about the optical axis of the reflected beam 208 is preferably tuned to match the tilt angle of the target object 178. Typically, AFM cantilevers are ^'tilted by an angle between 5 and 15 degrees. It is preferred to

position the physical pivot 120 location so as to induce a tilt in the conjugate Scheimpflug focal plane 180 that substantially matches the tilt angle of the cantilever. This allows the movement of the focused beam position along the cantilever by pitching or yawing the steering mirror 116 without the need to refocus the light beam, which would otherwise be required for the tilted

cantilever. In an alternative embodiment of this

invention, translation of the goniometric lens group 136 may be used to compensate for the tilt -angle of the cantilever. However, such an embodiment requires the use of three, instead of only two, motion transducers in order to retain a focused light spot on a tilted cantilever.

[0030] As depicted in FIG . 1, the light beam 208 reflected from the steering mirror 116 converges to a focus at the Scheimpflug surface 124 and subsequently diverges beyond that surface. The diverging light beam is then redirected by reflection off a fold be^'amsplitter 128. The fold beamsplitter 128 reflects part of the light beam, while allowing another part to traverse through the fold beamsplitter 128 to a photodetector 132 which measures the total amount of optical power in the light beam. The photodetector 132 can thus be used to tune the desired amount of light power by changing the drive current of the laser diode 100 accordingly or, as previously discussed, by rotating the linear polarizer 108 accordingly.

Typically, a small fraction of the light beam will be allowed to traverse through the fold beamsplitter 128 to the photodetector 132 and substantially all the light will be reflected from the fold beamsplitter 128. This

division of the light beam is preferable as it maintains a high optical power density at the target object 178.

[0031] The photodetector 132 discussed in the previous paragraph may be a linear position- sensitive detector, in which case the position of light beam on the photodetector 132 can be used to determine the axial position of the focus and the angular orientation of the light beam. A calibration procedure may also suffice to determine these two geometric factors. However a linear position-sensitive detector is desirable because it obviates the need for closed-loop position control on the actuators that produce pitch and yaw in the steering mirror 116. However if closed-loop position control is in any event provided, a linear position-sensitive detector complements such control and provides a reinforcing measure of the nominal position or center position of the steering mirror 116.

[0032] In the absence of a

photodetector 132, which is optional and not required for the functioning of the present invention, the fold

beamsplitter 128 only acts as a mirror redirecting the light. With a different orientation and position of the optical components, the fold beamsplitter 128 may not be necessary .

[0033] After reflecting from the fold beamsplitter 128, the light beam traverses one or a number of lenses that substantially collimate the beam 136. This group of lenses is referred to herein as the goniometric lens group. The number and kind of lenses used to

collimate the light beam depends on the exact optical parameters of the particular optical beam positioning unit under consideration as known to those skilled in the art. In the present invention the goniometric lens group 136 is provided with a means for translating the group along the optical axis to change the degree of collimation of the outgoing light beam. Moving the group backward or forward causes the light beam to be more divergent or convergent after traversing the group. This allows a user to adjust the axial position of the final focused spot relative to the target object 178.

[0034] One possible means of

translating the goniometric lens group 136 is by mounting the lens group in a threaded housing that is then rotated in a threaded bore. The mechanical motion of the lens group may be automated via a transducer, such as a motor, or manually adjusted by the user. The number of lenses in the group that may be required to move depends on the desired amount of collimation. The remainder of the lenses in the group, if any, may remain fixed.

[0035] The substantially collimated beam exiting the goniometric lens group 136 can be

attenuated, if necessary, by the use of a filter 140 that attenuates the light by a prescribed amount. The filter 140 may be a neutral density filter, a rotationally variable neutral density filter, a colored filter, or a linear polarizing filter. In any case, the filter 140 can be adjusted manually by the user or through an automated mechanism to change the desired amount of light

attenuation. Preferable automated mechanisms for this purpose include a rotationally variable neutral density filter on a motorized rotation stage and a motorized filter wheel with a plurality of filters, one of which is disposed in the beam. If filter 140 is a linear

polarizing filter, either the filter or the polarizer can be rotated either manually or by some motorized mechanism. In any case it is preferable to electronically identify which, if any, filter 140 is disposed in the light beam so that the resulting beam power may be readily available.

[0036] The light beam emerging from the filter 140 then traverses a polarizing beamsplitter 144 which passes only one polarization direction of the beam. The portion of the beam that is polarized in the

orthogonal direction to the desired polarization direction is reflected, rather than transmitted, and then absorbed by a beam dump 148, such as a black felt surface. The portion of the beam that is polarized in the desired polarization direction is transmitted to a quarter-wave plate 152 which converts the linearly polarized light beam transmitted into a circularly polarized light beam.

[0037] Optical elements in the path of the light beam after it exits the quarter-wave plate

152, such as the dichroic mirror 156, may introduce significant phase shifts between s-polarized and p- polarized light in the circularly polarized light beam. In this situation, the desired operation of the polarizing beamsplitter 144 and quarter wave plate 152 can be

achieved by replacing the quarter wave plate 152 with a waveplate having sufficient retardance greater or less than 0.25 waves) with suitable orientation so as to cancel the cumulative phase shift introduced by all subsequent optical elements in the light beam.

[0038] As noted above, the present invention may be used to measure the deflection or

oscillation of the probe as is common with AFMs and may also be employed to focus more than one light beam onto the probe (or the sample) to enable functionalities other than measuring probe displacement. When the present invention is used to achieve these other functionalities, detection of the reflected beam from the sample is not required. In this case, polarizing beamsplitter 144, waveplate 152 and beam stop 148, may be omitted without substantially changing the other aspects of the invention, and of course so also may the photodetector 182 used to measure the deflection or oscillation of the probe. In this connection it is necessary to remember that the presence or absence of the polarizing beamsplitter 144 and waveplate 152 have an important effect on the calculation of the correct distances in locating the virtual pivot 122 in the back focal plane 172 of objective lens 170.

[0039] Substantially all the circularly polarized light beam transmitted from the quarter-wave plate 152 is reflected from a dichroic mirror 156. Any portion of the beam that may traverse the dichroic mirror 156 is absorbed by a beam dump 160. A dichroic mirror is used here rather than a conventional mirror so that wavelengths other than the wavelengths in light source 100 will traverse the mirror 156 rather than being reflected, thereby allowing the camera system 186 to image light reflected from the target object 178.

[0040] The substantially collimated light beam exiting the dichroic mirror 156 then traverses another dichroic mirror 166, which allows for a light beam from another optical beam positioning unit to be combined into the light path, as will be described in more detail shortly. The collimated light beam then passes through an objective lens 170 that focuses the light beam close to the front focal plane 174 of the objective lens 170. The target object 178 targeted by the focused light beam is located close to the front focal plane ».74 of the

objective lens 170. The objective lens 170 may be a commercially available unit, such as the Olympus LUC Plan Fluor N 20x having a numerical aperture of 0.45, or it may be an objective lens designed specifically for use in this context. Typically, an objective lens for use in this context is composed of several optical components, some of which may be translated with respect to others to adjust the position of the front focal plane relative to the position of the lens, or to adjust the spherical

aberration correction of the focused spot. The preferred lens will have apochromatic or semi-apochromatic ("Fluor") correction of chromatic aberrations because it may be anticipated that multiple light beams c- different

wavelengths producing a plurality of spots will be

present. The preferred lens will also have flat field ("Plan") correction for off-axis aberrations because the camera system 186 will preferably incorporate a digital image sensor, and because it may be anticipated that the invention will be used in conjunction with planar samples such as silicon wafers.

[0041] Some portion cf the light beam focused by the objective lens 170 close to the front focal plane 174 of the lens will be reflected by the target object 178 also located close to the front focal plane

174. Another portion will be absorbed. It is also possible that some portion of the light beam will be transmitted through the target object 178, depending on the material and thickness of the target, object 178, and the wavelength of the light beam.

[0042] Some portion of the light beam reflected by the target object 178 will re-enter the objective lens 170 and return to a substantially

collimated state. The portion of the beam that re-enters the objective lens 170 may be maximized by laterally offsetting the incoming collimated beam in order to introduce a specific angle to the focus -;d light beam, as described in detail in US Patent No. 8,370,960, Modular Atomic Force Microscope, referred to above and

incorporated herein by reference. A substantial part, but not all, of the reflected light beam that re-enters the objective lens 170 reflects off the dichroic mirror 156 and is directed to the quarter-wave place 152 which then converts the circularly polarized returning light beam into a linearly polarized light beam. Because the

polarization orientation is now orthogonal to the

polarization orientation of the original light beam that previously crossed the quarter-wave plate 152 in the other direction, the light beam reflects off the polarizing beamsplitter 144 instead of traversing it. In the event that the quarter-wave plate 152 has been replaced by a waveplate having retardance and orientation to compensate for phase shifts in optical elements coming after the quarter-wave plate 152, as discussed above, the returning light beam reflects entirely off the polarizing

beamsplitter 144.

[0043] The reflected beam then impinges on a photodetector 182 which, when the present invention is being used to measure the deflection or oscillation of the probe, measures the position of the light beam. The two-dimensional position of the light beam on the photodetector 182 is used as a measure of the two- dimensional angular deviation of the target object 178 that reflected the light beam. However, when the present invention is employed to focus more than one light beam onto the probe (or the sample) to enable functionalities other than measuring probe displacement, the photodetector 182 can be used to measure the light power of the light beam.

[0044] A portion of the light beam that re-enters the objective lens 170 will t.-averse the

dichroic mirror 156 and can be imaged using the camera system 186 if the target object 178 is illuminated by an appropriate light source, preferably a white light source. The camera system 186 can also image the focused light spot reflected from the target object 178.

[0045] In order to assure that the brightness of the target object 178 is similar in

magnitude to the brightness of the reflected light beam entering the camera system 186, a color filter 190 can be used to selectively dim the light beam to any degree necessary. It may also be necessary to adjust the

exposure time and aperture size provided by the camera system 186 to obtain proper exposure of the target object 178 and focused light spot. Even if only a small amount of the reflected light beam traverses the dichroic mirror 156 to the camera system 186 the beam will appear very bright due to its high power density. Therefore, it is anticipated that the filter 190 will be necessary to provide a good quality image in the camera system 186.

[0046] As described earlier,

translating the goniometric lens group 136 along its axis changes the axial location of the final focused spot relative to the front focal plane 174 of the objective lens 170. Prior art would have positioned the light beam focus in the axial direction by either moving the

objective lens 170, or moving the target object 178, or both. The present invention allows the light beam to be focused without moving the objective lens 170 or the target object 178. The same will be true for the light beam from another optical beam position unit (or units), with their own goniometric lens groups 136. The light beams of such other units would enter the light path via other dichroic mirrors located between the dichroic mirror 156 of the first optical beam positioning unit and the objective lens 170. FIG. 1 shows one such dichroic mirror 166. FIG. 2 shows a light path with a multiplicity of optical beam position units and dichroi mirrors. The additional dichroic mirrors, starting with dichroic mirror 166, may have different optical specifications than dichroic mirror 156 in order to optimally combine

different light beams while minimizing loss of light power. When the goniometric lens group 136 of any one of such units is translated the axial location of the final focused spot of the relevant unit relative to the front focal plane 174 of the objective lens 170 is changed without either moving the objective lens 170, or moving the target object 178, or both. This is a significant difference relative to prior art because moving the objective lens 170, or moving the target object 178, or both, in order to position the light beam focus in the axial direction for the benefit of one optical beam positioning unit could significantly degrade the

performance of other optical beam positioning units.

FIG. 3 shows another variation of the FIG. 2 arrangement with multiplicity of optical beam position units feeding into a single light path. In FIG. 3 two or more optical beam position units have their translat ional degrees of freedom coupled so that their respective focused light spots move together in a desired direction. For example, a secondary optical beam position unit may be tethered to a primary optical beam position unit so that their focused light spots move together when the focused light spot of the primary optical beam position unit is translated in one or more directions, while the focused light spot of secondary optical beam position unit is also moved focused independently relative to the focused light spot of the primary optical beam position unit. Although FIG. 3 represents three nested optical beam position units, any number of degrees of freedom between any number of optical beam position units may be coupled as preferred by the user .

Photothermal Excitation

[0047] In addition to being used to measure the deflection or oscillation of the probe, the light beam of an optical beam positioning unit can be used to photothermally excite mechanical vibrations of the probe. For this purpose light at the blue end of the visible spectrum is preferred. The inventors have used the beam from a laser emitting light with a wave length of 405 nm with satisfactory results.

[0048] In the prior art, a coating on some or all of the cantilever portion of the probe was required to convert heat from the light beam into

mechanical strain in the probe, via different thermal expansion coefficients of the coated portion of the cantilever and the remainder of the probe. While the current invention is compatible with such coated

cantilevers, it does not require a coating to

photothermally induce mechanical vibrations of the probe. Due to the well-corrected optical design, the light beam focus in the present invention is significantly smaller than in the prior art. The smaller light beam focus produces larger thermal gradients that cause photothermal excitation even in probes fabricated from a single

material. Because the material of the probe has nonzero thermal expansion, the thermal gradients produce strain gradients, especially when the light beam power is

modulated to produce time-varying temperature gradients. For example, the light beam power can be changed

sinusoidally as a function of time, producing a sinusoidal mechanical motion as required for amplitude-modulated atomic force microscopy. As described in prior art, such mechanical motion or vibration may also be enhanced by differences in thermal coefficients of expansion of two or more materials composing the probe in the case of

heterogeneous probes.

[0049] Photothermal excitation of the probe may also be used in conjunction with other methods to form hybrid modes of cantilever excitation. For

example, the cantilever may be driven by piezoacoustic excitation at a first resonance while simultaneously driven by photothermal excitation at a second resonance. This combination is useful if a large amplitude of

oscillation, achievable with piezoacoustic excitation, is necessary for a first resonance, while the clean response of photothermal excitation is preferable for driving a second resonance. Alternative schemes for excitation may be developed to meet specific experimental goals. For example, photothermal excitation could be used to excite mechanical motion at a resonance of the cantilever while piezoacoustic excitation is used to drive the cantilever at a frequency that is not close to a cantilever

resonance. Some of these schemes of excitation may involve frequency modulation or frequency tracking, in order to measure mechanical parameters of the sample, the probe or the tip of the probe. In this case, photothermal excitation is known in the prior art to provide an

advantage because it provides a transfer function from excitation voltage to mechanical motion that is

substantially independent of frequency and free from spurious resonances. [0050] The location of the focused light beam on the probe used for photothermal excitation affects the drive amplitude of the probe. The relationship between location and drive amplitude is also frequency dependent because the probe has a frequency response composed of many normal and torsional eigenmodes. There are locations that provide zero excitation of the second eigenmode, while providing non-zero excitation of the first eigenmode, for example. Depending on the experiment, it may be desirable to tune the drive amplitude of the probe at different frequencies. This may be achieved by modulating the power of the photothermal excitation light beam at particular frequencies, that may correspond to different eigenmodes of the probe, while rastering the focused spot relative to the probe and measuring the driven amplitude. It may be desirable, :or example, to maximize the torsional (or normal) vibration response of the probe, while minimizing the response of the normal (or torsional) deflection of the probe in certain experiments.

[0051] FIG. 8 shows an amplitude map of an ArrrowUHFAuD cantilever as a function of blue laser excitation near the probe. The amplitude response is also function of the modulation frequency of the blue light power. Modulating the light power at frequencies near the I^s eigenmode and 2 eigenmode of the probe, as shown in both images, induces different bending modes of the cantilever which have different location-dependent

responses. The width and height of each map is 66

microns. The outline of the cantilever :.s also drawn for reference .

blueTherm

[0052] The light beam of an optical beam positioning unit can also be used to heat certain parts of the target object 178 to a varying degree. For this purpose light at the blue end of the visible spectrum is preferred. The inventors have used the beam from a laser emitting light with a wave length of 405 nm with satisfactory results.

[0053] Heating certain parts of the target object 178 to a varying degree causes a desirable steady-state temperature gradient to form in the probe or the sample at time scales that are longar than the

mechanical time constant of the object being heated. Such a steady-state temperature gradient may occur while vibrations of the target object 178 are being induced by modulating the light beam at faster time scales. In any case, with the probe the temperature gradient can be optimized by adjusting the total optical power of the light beam and the beam position relative to the probe to control the temperature of a certain part of the probe, such as the tip of the cantilever. The variable

temperature of the probe and the tip of the cantilever can be used to induce thermally activated changes in the sample according to any desired experimental protocol.

One category of such experiments is knov/n as local thermal analysis. In the prior art it is carried out using a special probe with heating elements and even a thermometer microfabricated in the probe. Such special probes are costly and are only available in a few spring constant values. With the present invention however such special probes are unnecessary as the focused light beam of the optical beam positioning unit can heat any existing probe useful for a local thermal analysis experiment.

[0054] Although the present invention can photothermally excite mechanical motion in uncoated probes, it may still be beneficial to optimize the optical and thermal

properties of the probe for heating with the light beam of an optical beam positioning unit. For example, the

reflectivity of the coating of the probe may be tuned appropriately, and the thermal conductivity of the probe may be patterned, through selective doping, in order to allow heat to flow to the tip of the cantilever more readily than to flow to the base of the probe (or vice versa) . Furthermore, the probe may be shaped in order to facilitate conduction of heat to the tip of the

cantilever; for example, the tip of the cantilever may be hollow such that the incident beam is absorbed closer to the tip. Patterning a metallic coating on the probe or the tip of the cantilever may also be used to maximize the heat flow to the tip to attain higher tip temperatures for a given light power. Coating only the end of the probe near the tip, while keeping the bulk of the probe

uncoated, may also be beneficial by reducing unwanted bending of the probe caused by thermal expansion

coefficient differences between the coating material and the probe material.

[0055] It is beneficial to measure the temperature of the probe for several types of experiments, such as calibrating the spring constant based on thermomechanical motion. In the present invention, the temperature of the probe may be quantified by measuring the change in the resonant frequency of the probe while changing the power of the light beam, by turning the light beam on and off for example. The temperature of the probe is related to its resonant frequency because Young' s modulus of the probe is dependent on the temperature of the probe.

Furthermore, the residual stress in coaced probe may have a temperature dependence that can impact the relationship between the resonant frequency and the temperature. The temperature may also be inferred by measuring the

deflection of the cantilever before and after turning the light beam on (or off) .

[0056] Frequency-modulated AFM (F -AFM) imaging has garnered a great deal of attention because of its high spatial resolution in air, vacuum and e>^ren fluid. Even with the prior art, FM-AFM has demonstrated success at imaging single atomic defects, imaging individual chemical bonds between surface atoms and measuring the force as a single atom was moved across a surface.

[0057] FM-AFM also permits simultaneous measurement of dissipative interactions between the tip of the cantilever and the sample. Dissipation is a combined effect of multiple interactions including long range electrostatic and magnetic interactions, as well as hysteretic

interatomic forces associated with the approach and retraction of the tip of the cantilever from the surface. In the prior art the measurement of dissipation has been unreliable. Dissipation is difficult t> quantify and relate to underlying physical mechanisms and results have not been reproducible. One important feature required for accurate dissipation measurements is frequency independent probe excitation. Specifically, coupling between the drive amplitude and frequency can lead to spurious

dissipation measurements. Piezoacoustic excitation, especially in liquid, suffers from a "forest of peaks" in the transfer function between the excitation voltage and the mechanical motion. These peaks are caused by spurious resonances in the mechanical system, such as the

resonances of a cantilever holder or liquid droplet. The peaks are not reproducible from one experiment to the next, and can even drift during one experiment. Because of these peaks, piezoacoustic excitation is very

frequency-dependent, sometimes changing by a factor of ten within the probe bandwidth. Since the photothermal drive described here is substantially constant or at the least varies only slowly over typical operational frequencies, it provides more accurate dissipation measurements than the piezoacoustic excitation commonly used in the prior art and is much more reproducible. blueClean [0058] After what is usually substantial use,

AFM probes become contaminated by interaction with the sample. Contaminated probes must either be replaced by new probes or where feasible cleaned. In typical laboratory settings, probes are often cleaned ex situ using an assortment of chemical solutions, sometimes combined with UV exposure. In prior art, a dedicated apparatus was often used for cleaning probes. The cleaning of probes

frequently involves a significant amount of time, and often is not successful. However replacing a contaminated probe with a new one can cost a significant amount of money. In either case, the experiment must be halted to remove and replace the existing probe from the AFM.

Removing and replacing the probe also loses information regarding the sample location being imaged.

[0059] Heating the probe may be used as a method of cleaning the tip of the cantilever. For this purpose light at the blue end of the visible spectrum is preferred. The inventors have used the beam from a laser emitting light with a wave length of 405 nm with

Sflti srtriru rpqiill-ς

[0060] Before, during, or after imaging a sample, the light beam of an optical beam positioning unit may be turned on momentarily to heat the tip of the cantilever in order to modify the tip coating or to break down, thermally modify or remove contaminations that have adhered to the tip. This method of cleaning or modifying the tip of the cantilever has the advantage over prior art that the process can be performed in situ, while the probe is in close proximity to the sample. The fact that the tip of the cantilever can be cleaned or modified without removing it from the AFM is a time-saving and important improvement in that it allows the continuation of the experiment after cleaning or modification without any cumbersome repositioning of the probe.

[0061] A well-parameterized probe is very important in nanomechanical measurements, such as stiffness, storage and loss moduli, loss tangents,

adhesion, indentation and a host of other parameters known to one skilled in the art. For this application a clean probe is of great importance.

Sample Modification

[0062] The light beam of an optical beam positioning unit may be used for inducing

photochemical, photovoltaic, photothermal , pyroelectric or other light sensitive changes to specific portions of the sample. For photothermal changes light at the blue end of the visible spectrum is preferred. The inventors have used the beam from a laser emitting light with a wave length of 405 nm with satisfactory results. For

photochemical, photovoltaic and pyroelectric changes light with a variety of wave lengths is satisfactory.

[0063] Changes of this character may be accomplished either with the beam positioned on the probe or with the beam off the probe. The positioning of the light beam allows the user to select the locations of the sample that may undergo such changes, while the total power of the light beam can be tuned to vary the degree of changes induced in the chosen sample location. Moving the position of the focused light beam in two or three

dimensions while varying the light power of the light beam enables the preparation or modification of the AFM sample before, during, or after imaging. blueTracking

[0064] As discussed above, in an AFM changes in the oscillation amplitude of the probe are typically made to trigger a change in the vertical position of the base of the probe relative to the sample (referred to herein as a change in the Z position, where Z is generally orthogonal to the X/Y plane defined by the sample) , in order to maintain the oscillation amplitude at a constant pre-set value. It is this feedback that is typically used to generate an AFM image as the probe is rastered above the sample surface.

[0065] In some instances however mechanical resonances of the AFM hardware limit the response time of tracking the Z position of the sample topography. Attempting to track the surface of the sample with a frequency bandwidth that exceeds these mechanical resonances leads to oscillations that prevent accurate topography tracking and damage the tip of the cantilever.

[0066] This issue can be avoided by using the light beam to induce deliberate sub-resonance bending of the cantilever. Bending a cantilever upwards or downwards is analogous to moving the sample downwards or upwards. Because the mechanical resonance of a small cantilever greatly exceeds the mechanical resonance of the AFM mechanical hardware, the feedback for keeping constant oscillation amplitude for accurate topography tracking can be operated at very high frequencies - upwards of 100kHz. [0067] For this purpose light at the blue end of the visible spectrum is preferred. The inventors have used the beam from a laser emitting light with a wave length of 405 nm on a gold coated cantilever with satisfactory results. Other wavelengths may be preferable if the cantilever is uncoated or coated with a material other than gold.

[0068] By varying the light power at time scales slower than the oscillation period of the cantilever, the cantilever bending can be approximated as linear with the light power. The light power is thus used to change the distance between the tip of the cantilever and the sample in order to track topography changes while rastering the tip over the sample surface. This device for tracking topography changes can be used in conjunction with the optical lever of an AFM, or can entirely replace the optical lever. In other words, as che sample is moved in the x and y direction for scanning, the cantilever bending resulting from the use of light power changes can be used to maintain the conditions for topography

tracking. For example, in the case of amplitude modulated AFM, the on-resonance amplitude of oscillation can be held constant by the aid of a feedback loop that changes the cantilever bending through the use of light power changes. This method has the additional advantage that the same light beam can be used to oscillate the cantilever on resonance while the average light power is independently modulated to track the sample topography.

[0069] Although only a few embodiments have been disclosed in detail above, other embodiments are possible and the inventors intend these to be encompassed within this specification. The specification describes specific examples to accomplish a more general goal that may be accomplished in another way. This disclosure is intended to be exemplary, and the claims are intended to cover any modification or alternative which might be predictable to a person having ordinary skill in the art. For example, other devices, and forms of modularity, can be used.

[0070] Also the inventors intend that only those claims which use the words "means for" are intended to be interpreted under 35 USC 112, sixth

paragraph. Moreover, no limitations from the specification are intended to be read into any claims_., unless those limitations are expressly included in the claims. The computers described herein may be any kind of computer, either general purpose, or some specific purpose computer such as a workstation. The computer may be a Pentium class computer, running Windows XP or Linux, or may be a

Macintosh computer. The computer may al.so be a handheld computer, such as a PDA, cellphone, or laptop.

[0071] The programs may be written in

C, or Java, Brew or any other programming language. The programs may be resident on a storage medium, e.g., magnetic or optical, e.g. the computer hard drive, a removable disk or media such as a memory stick or SD media, or other removable medium. The programs may also be run over a network, for example, with a server or other machine sending signals to the local machine, which allows the local machine to carry out the operations described herein .

Claims

What is claimed is:

1. An atomic force microscope system with two optical beam positioning units operating to characterize a sample,

comprising : an atomic force microscope probe with a tip at one end of the cantilever; a sample which is located below the tip oZ the cantilever; an objective lens which allows optical viewing in an area of the cantilever or the sample, directs a light beam to the back of the cantilever opposite the tip and obtains a return beam from the cantilever indicative of the movement of the cantilever; two dichroic mirrors, one of which receives a beam from the first optical beam positioning unit and in turn directs the beam to the objective lens, and the other of which receives a beam from the second optical beam positioning unit and in turn directs the beam to the objective lens; a first optical beam positioning unit, comprising: a light source with a lens emitting an infrared beam directed at a steering mirror that is rotatable in two orthogonal axes that are parallel to the mirror's surface, one of such axes lying within the plane of incidence wherein lie both the infrared beam and the reflection of such beam; focusing the axial position of the infrared beam reflected from the mirror on the back of the cantilever opposite the tip by pitching and yawing t e steering mirror so that the physical pivot where the two orthogonal axes intersect coincides with point of incidence where the infrared beam is reflected from the mirror; a lens group which collimates the infrared beam reflected from the steering mirror and directs it to a polarizing beamsplitter and quarter-waveplate and from which it is directed outside the first optical beam

positioning unit to a dichroic mirror; and the quarter-waveplate and polarizing beamsplitter which receive from the objective lens the return beam from the cantilever indicative of the movement of the cantilever and direct the beam to a photodetector ; a second optical beam positioning unit, comprising: a light source with a lens emitting an beam in the blue end of the visible spectrum directed at a steering mirror that is rotatable in two orthogonal axes that are parallel to the mirror's surface, one of such axes lying within the plane of incidence wherein lie both the blue beam and the reflection of such beam; focusing the axial position of the blue beam reflected from the mirror on the base of the probe opposite the tip by pitching and yawing the steering mirror so that the physical pivot where the two orthogonal axes intersect coincides with point of incidence where the infrared beam is reflected from the mirror; and a lens group which collimates the blue beam reflected from the steering mirror and directs it outside the second optical beam positioning unit to a dichroic mirror.

2. The system as in claim 1 wherein said beam in the blue end of the visible spectrum is a beam with a wave length of 405 nm.